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MATSUOKA Satoshi
| Comprehensive Analysis Center for Science | Associate Professor |
| Research Promotion Office | Director |
Researcher information
■ Research Keyword■ Field Of Study
■ Career
- Oct. 2021 - Present, Saitama University, Graduate School of Science and Engineering, Associate Professor
- Oct. 2017 - Sep. 2021, Saitama University, Graduate School of Science and Engineering
- Jan. 2008 - Sep. 2017, Saitama University, Graduate School of Science and Engineering
- Aug. 2005 - Dec. 2007
- Apr. 2002 - Mar. 2005, Saitama University, Graduate School of Science and Engineering
- Apr. 2000 - Mar. 2002, Saitama University, Graduate School of Science and Engineering
- Apr. 1996 - Mar. 2000, Saitama University, Faculty of Science
Performance information
■ Paper- Galactolipids from Arabidopsis thaliana can replace the function of glucolipids in Bacillus subtilis.
Manami Kawakami; Satoshi Matsuoka
The Journal of General and Applied Microbiology, Apr. 2022, [Reviewed], [Corresponding]
DOI：https://doi.org/10.2323/jgam.2021.08.002
DOI ID：10.2323/jgam.2021.08.002 - Glucolipids and lipoteichoic acids affect the activity of SigI, an alternative sigma factor, and WalKR, an essential two-component system, in Bacillus subtilis.
Satoshi Matsuoka; Yoko Shimizu; Kaori Nobe; Kouji Matsumoto; Kei Asai; Hiroshi Hara
Genes to cells, Volume：27, Number：2, First page：77, Last page：92, Dec. 2021, [Reviewed], [Lead, Corresponding], ［International magazine］
In a Bacillus subtilis ugtP mutant lacking glucolipids, SigI was activated in the log phase, and the activation of SigI in the mutant was suppressed by the expression of native ugtP. By contrast, SigI was inhibited in a yfnI mutant lacking one of the lipoteichoic acid (LTA) synthase genes, and the inhibition was suppressed by the expression of yfnI. A series of mutation analyses of the sigI promoter revealed that the two WalR binding sites were involved in the increase of PsigI -lacZ activity in the ugtP mutant and decrease of the lacZ activity in the yfnI mutant. Transcription from the SigI recognition sequence was enhanced in the ugtP mutant, whereas yfnI disruption inhibited the transcription from the SigA recognition sequence in the sigI promoter. We found that not only SigI but also WalKR, the essential two-component system, was activated in the ugtP mutant and inhibited in the yfnI mutant. The walK mutants with activated WalR exhibited abnormal morphology, but this phenotype was suppressed by the addition of MgSO4 . We conclude that glucolipids and LTA are key compounds in the maintenance of normal cell surface structure in B. subtilis.
English, Scientific journal
DOI：https://doi.org/10.1111/gtc.12912
DOI ID：10.1111/gtc.12912, PubMed ID：34910349 - Establishment of a cell-free translation system from rice callus extracts
Kakeru Suzuki; Haruka Inoue; Satoshi Matsuoka; Ryugo Tero; Ayumi Hirano-Iwata; Yuzuru Tozawa
Bioscience, Biotechnology, and Biochemistry, Volume：84, Number：10, First page：2028, Last page：2036, Oct. 2020, [Reviewed]Abstract
Eukaryotic in vitro translation systems require large numbers of protein and RNA components and thereby rely on the use of cell extracts. Here we established a new in vitro translation system based on rice callus extract (RCE). We confirmed that RCE maintains its initial activity even after five freeze-thaw cycles and that the optimum temperature for translation is around 20°C. We demonstrated that the RCE system allows the synthesis of hERG, a large membrane protein, in the presence of liposomes. We also showed that the introduction of a bicistronic mRNA based on 2A peptide to RCE allowed the production of two distinct proteins from a single mRNA. Our new method thus facilitates laboratory-scale production of cell extracts, making it a useful tool for the in vitro synthesis of proteins for biochemical studies.
Informa UK Limited, Scientific journal
DOI：https://doi.org/10.1080/09168451.2020.1779024
DOI ID：10.1080/09168451.2020.1779024, ISSN：0916-8451, eISSN：1347-6947 - Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.
Seki T; Furumi T; Hashimoto M; Hara H; Matsuoka S
Genes & genetic systems, Volume：94, Number：2, First page：71, Last page：80, Apr. 2019, [Reviewed], [Corresponding], ［Domestic magazine］
In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA). LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane. Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA. Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone. Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively). These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.
English, Scientific journal
DOI：https://doi.org/10.1266/ggs.18-00046
DOI ID：10.1266/ggs.18-00046, ISSN：1341-7568, PubMed ID：30971625 - Role of the inner-membrane histidine kinase RcsC and outer-membrane lipoprotein RcsF in the activation of the Rcs phosphorelay signal transduction system in Escherichia coil
Takatsugu Sato; Akira Takano; Nanako Hori; Tomoko Izawa; Takayoshi Eda; Kota Sato; Mitsuru Umekawa; Hiroyoshi Miyagawa; Kenji Matsumoto; Ayako Muramatsu-Fujishiro; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
MICROBIOLOGY-SGM, Volume：163, Number：7, First page：1071, Last page：1080, Jul. 2017, [Reviewed]
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. The outer-membrane lipoprotein RcsF is an essential component of the Rcs system. Mislocalization of RcsF to the periplasm or the cytoplasmic membrane leads to high activation of the Rcs system, suggesting that RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. This is consistent with the result reported by Cho et al. (Cell 1 59, 1652-1664,2014) showing that RcsF interacts with the periplasmic domain (YrfFperi) of the inner-membrane protein YrfF (IgaA in Salmonella enterica serovar Typhimurium), which is a negative regulator of the Rcs system. In this study we show that RcsF also interacts with the periplasmic domain of the innermembrane-localized histidine kinase RcsC (RcsCperi). RcsCperi, which was secreted to the periplasm by fusion to maltose-binding protein, titrated RcsF's activating effect. A bimolecular fluorescence complementation experiment showed interaction of RcsF with RcsCperi, as well as with YrfFperi. We conclude that RcsF interacts with the periplasmically exposed region of RcsC, as well as with that of YrfF.
MICROBIOLOGY SOC, English, Scientific journal
DOI：https://doi.org/10.1099/mic.0.000483
DOI ID：10.1099/mic.0.000483, ISSN：1350-0872, eISSN：1465-2080, PubMed ID：28691662, Web of Science ID：WOS:000409533400014 - Activation without Proteolysis of Anti-sigma Factor RsiV of the Extracytoplasmic Function sigma Factor sV in a Glucolipid-Deficient Mutant of Bacillus subtilis
Seki Takahiro; Matsumoto Kouji; Matsuoka Satoshi; Hara Hiroshi
ADVANCES IN MICROBIOLOGY, Volume：7, Number：4, First page：315, Last page：327, Apr. 2017, [Reviewed]
Scientific Research Publishing, Inc., Scientific journal
DOI：https://doi.org/10.4236/aim.2017.74026
DOI ID：10.4236/aim.2017.74026, ISSN：2327-0810, eISSN：2165-3410, Web of Science ID：WOS:000407172900009 - Septal membrane localization by C-terminal amphipathic alpha-helices of MinD in Bacillus subtilis mutant cells lacking MinJ or DivIVA
Kazuki Ishikawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：92, Number：2, First page：81, Last page：98, Apr. 2017, [Reviewed]
The Min system, which inhibits assembly of the cytokinetic protein FtsZ, is largely responsible for positioning the division site in rod-shaped bacteria. It has been reported that MinJ, which bridges DivIVA and MinD, is targeted to the cell poles by an interaction with DivIVA, and that MinJ in turn recruits MinCD to the cell poles. MinC, however, is located primarily at active division sites at mid-cell when expressed from its native promoter. Surprisingly, we found that Bacillus subtilis MinD is located at nascent septal membranes and at an asymmetric site on lateral membranes between nascent septal membranes in filamentous cells lacking MinJ or DivIVA. Bacillus subtilis MinD has two amphipathic alpha-helices rich in basic amino acid residues at its C-terminus; one of these, named MTS1 here, is the counterpart of the membrane targeting sequence (MTS) in Escherichia coli MinD while the other, named MTS-like sequence (MTSL), is the nearest helix to MTS1. These amphipathic helices were located independently at nascent septal membranes in cells lacking MinJ or DivIVA, whereas elimination of the helices from the wild type protein reduced its localization considerably. MinD variants with altered MTS1 and MTSL, in which basic amino acid residues were replaced with proline or acidic residues, were not located at nascent septal membranes, indicating that the binding to the nascent septal membranes requires basic residues and a helical structure. The septal localization of MTSL, but not of MTS1, was dependent on host cell MinD. These results suggest that MinD is targeted to nascent septal membranes via its C-terminal amphipathic alpha-helices in B. subtilis cells lacking MinJ or DivIVA. Moreover, the diffuse distribution of MinD lacking both MTSs suggests that only a small fraction of MinD depends on MinJ for its localization to nascent septal membranes.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.16-00054
DOI ID：10.1266/ggs.16-00054, ISSN：1341-7568, eISSN：1880-5779, PubMed ID：28674273, Web of Science ID：WOS:000417966000003 - Biological functions of glucolipids in bacillus subtilis
Satoshi Matsuoka
Genes and Genetic Systems, Volume：92, Number：5, First page：217, Last page：221, 2017, [Reviewed], [Invited], [Corresponding]
Glyceroglycolipids are very important in Gram-positive bacteria and cyanobacteria. In Bacillus subtilis, a model organism for the Gram-positive bacteria, the ugtP mutant, which lacks glyceroglucolipids, shows abnormal morphology. Lack of glucolipids has many consequences: abnormal localization of the cytoskeletal protein MreB and activation of some extracytoplasmic function (ECF) sigma factors (σM, σV and σX) in the log phase are two examples. Conversely, the expression of monoglucosyldiacylglycerol (MGlcDG) by 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii (alMGS) almost completely suppresses the ugtP disruptant phenotype. Activation of ECF sigmas in the ugtP mutant is decreased by alMGS expression, and is suppressed to low levels by MgSO4 addition. When alMGS and alDGS (A. laidlawii 1,2-diacylglycerol-3-glucose (1-2)-glucosyltransferase producing diglucosyldiacylglycerol (DGlcDG)) are simultaneously expressed, σX activation is repressed to wild type level. These observations suggest that MGlcDG molecules are required for maintenance of B. subtilis cell shape and regulation of ECF sigmas, and that DGlcDG regulates σX activity. The activation of ECF sigmas is not accompanied by proteolysis of anti-σ. Thus, glyceroglucolipids may have the specific role of helping membrane proteins function by acting in the manner of chaperones.
Genetics Society of Japan, English
DOI：https://doi.org/10.1266/ggs.17-00017
DOI ID：10.1266/ggs.17-00017, ISSN：1880-5779, SCOPUS ID：85045191467, Web of Science ID：WOS:000434493000002 - Septal localization by membrane targeting sequences and a conserved sequence essential for activity at the COOH-terminus of Bacillus subtilis cardiolipin synthase
Jin Kusaka; Satoshi Shuto; Yukiko Imai; Kazuki Ishikawa; Tomo Saito; Kohei Natori; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
RESEARCH IN MICROBIOLOGY, Volume：167, Number：3, First page：202, Last page：214, Apr. 2016, [Reviewed]
The acidic phospholipid cardiolipin (CL) is localized on polar and septal membranes and plays an important physiological role in Bacillus subtilis cells. ClsA, the enzyme responsible for CL synthesis, is also localized on septal membranes. We found that GFP fusion proteins of the enzyme with NH2-terminal and internal deletions retained septal localization. However, derivatives with deletions starting from the COOH-terminus (Leu482) ceased to localize to the septum once the deletion passed the Ile residue at 448, indicating that the sequence responsible for septal localization is confined within a short distance from the COOH-terminus. Two sequences, Ile436-Leu450 and Leu466-Leu478, are predicted to individually form an amphipathic a-helix. This configuration is known as a membrane targeting sequence (MTS) and we therefore refer to them as MTS2 and MTS1, respectively. Either one has the ability to affect septal localization, and each of these sequences by itself localizes to the septum. Membrane association of the constructs of this enzyme containing the MTSs was verified by subcellular fractionation of the cells. CL synthesis, in contrast, was abolished after deleting just the last residue, Leu482, in the COOH-terminal four amino acid residue sequence, Ser-Pro-Ile-Leu, which is highly conserved among bacterial CL synthases. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
ELSEVIER SCIENCE BV, English, Scientific journal
DOI：https://doi.org/10.1016/j.resmic.2015.11.004
DOI ID：10.1016/j.resmic.2015.11.004, ISSN：0923-2508, eISSN：1769-7123, Web of Science ID：WOS:000372890100006 - Suppression of abnormal morphology and extracytoplasmic function sigma activity in Bacillus subtilis ugtP mutant cells by expression of heterologous glucolipid synthases from Acholeplasma laidlawii
Satoshi Matsuoka; Takahiro Seki; Kouji Matsumoto; Hiroshi Hara
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：80, Number：12, First page：2325, Last page：2333, 2016, [Reviewed], [Lead, Corresponding]
Glucolipids in Bacillus subtilis are synthesized by UgtP processively transferring glucose from UDP-glucose to diacylglycerol. Here we conclude that the abnormal morphology of a ugtP mutant is caused by lack of glucolipids, since the same morphology arises after abolition of glucolipid production by disruption of pgcA and gtaB, which are involved in UDP-glucose synthesis. Conversely, expression of a monoglucosyldiacylglycerol (MGlcDG) produced by 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii (alMGS) almost completely suppressed the ugtP disruptant phenotype. Activation of extracytoplasmic function (ECF) sigmas (SigM, SigV, and SigX) in the ugtP mutant was decreased by alMGS expression, and was suppressed to low levels by MgSO4 addition. When alMGS and alDGS (A. laidlawii 1,2-diacylglycerol-3-glucose (1-2)-glucosyltransferase producing diglucosyldiacylglycerol (DGlcDG)) were simultaneously expressed, SigX activation was repressed to wild type level. These observations suggest that MGlcDG molecules are required for maintenance of B. subtilis cell shape and regulation of ECF sigmas, and DGlcDG regulates SigX activity.
TAYLOR & FRANCIS LTD, English, Scientific journal
DOI：https://doi.org/10.1080/09168451.2016.1217147
DOI ID：10.1080/09168451.2016.1217147, ISSN：0916-8451, eISSN：1347-6947, Web of Science ID：WOS:000386387900007 - Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells
Takahiro Seki; Ryota Mineshima; Michihiro Hashimoto; Kouji Matsumoto; Hiroshi Hara; Satoshi Matsuoka
Genes and Genetic Systems, Volume：90, Number：2, First page：109, Last page：114, Sep. 2015, [Reviewed], [Last]
Extracytoplasmic function (ECF) σ factors respond to environmental stresses and regulate numerous genes required for adaptation. Under normal growth conditions, the ECF σ factors are sequestered by transmembrane anti-σ factor proteins, from which they are released under stress conditions. In Bacillus subtilis ugtP null mutant cells, which lack glucolipids, three of the seven ECF σ factors, σM, σV and σX, are activated. The Escherichia coli cell membrane does not contain glucolipids. When the genes for these three ECF σ and anti-σ factors were introduced into E. coli cells, expression of lacZ fused to the ECF σ factor-regulated promoters indicated ECF σ factor activity. Additional expression of the ugtP gene in these E. coli cells led to the synthesis of small amounts of glucolipids, and the activities of σM and σV were repressed, but the activity of σX was unaffected. It is likely that glucolipids directly influence anti-σM and anti-σV factors by stabilizing conformations that sequester the respective ECF σ factors.
Genetics Society of Japan, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.90.109
DOI ID：10.1266/ggs.90.109, ISSN：1880-5779, PubMed ID：26399770, SCOPUS ID：84937019396 - Importance of the proline-rich region for the regulatory function of RcsF, an outer membrane lipoprotein component of the Escherichia coli Rcs signal transduction system
Mitsuru Umekawa; Hiroyoshi Miyagawa; Daitetsu Kondo; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
Microbiology (United Kingdom), Volume：159, Number：9, First page：1818, Last page：1827, Sep. 2013, [Reviewed]
The outer membrane lipoprotein RcsF is an essential component of the Rcs phosphorelay signal transduction system in Escherichia coli. It senses stresses imposed on the cell envelope and conveys the information to histidine kinase RcsC in the cytoplasmic membrane. Mislocalization of RcsF to the periplasm, effected by fusing it to the periplasmic maltose-binding protein, or to the cytoplasmic membrane, brought about by changing the lipoprotein sorting signal, leads to high activation of the Rcs system, suggesting that RcsF functions as a ligand for RcsC in activating the system. Here, we focus on the proline-rich region (PRR) in the N-terminal half of RcsF, a region which also contains many basic amino acid residues. Deletion of the PRR in the mislocalized RcsF resulted in even higher activation of the Rcs system. The same deletion in wild-type RcsF lipoprotein that is correctly localized to the outer membrane, however, blocked activation of the system under stresses that normally should activate it. It is highly likely that the PRR plays an important role in the regulation of the function of RcsF in activating the Rcs system. © 2013 SGM.
English, Scientific journal
DOI：https://doi.org/10.1099/mic.0.069328-0
DOI ID：10.1099/mic.0.069328-0, ISSN：1350-0872, PubMed ID：23813676, SCOPUS ID：84883366818 - Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defects in lipoteichoic acid synthesis
Michihiro Hashimoto; Takahiro Seki; Satoshi Matsuoka; Hiroshi Hara; Kei Asai; Yoshito Sadaie; Kouji Matsumoto
MICROBIOLOGY-SGM, Volume：159, First page：23, Last page：35, Jan. 2013, [Reviewed]
Lipoteichoic acid (LTA) is an important cell envelope component of Gram-positive bacteria. Bacillus subtilis has four homologous genes for LTA synthesis: ltaS (yflE), yfnI, yqgS and yvgJ. The products LtaS (YflE), YfnI and YqgS are bona fide LTA synthetases, whereas YvgJ functions only as an LTA primase. To clarify whether defects in LTA on the cell envelope trigger extracytoplasmic function (ECF) sigma factors, mRNA levels of the autoregulated ECF sigma factors in cells with singly and multiply deleted alleles of the ltaS homologues were examined by real-time RT-PCR. This revealed that sigM and sigX were induced in cells with a null allele of Delta ltaS and Delta yfnI, respectively, and that no ECF sigma factor was induced in cells with a single null allele of Delta yqgS or Delta yvgJ. In cells with double null alleles (Delta ltaS and Delta yfnI), sigW and ylaC were induced in addition to sigM and sigX. Cells with triple null alleles (Delta ltaS Delta yfnI and Delta yqgS) showed a pattern of induction similar to that of the double null. In cells with quadruple null alleles, sigV and sigY were newly induced. Cells with Delta ltaS had approximatley 1/4 the diglucosyldiacylglycerol and over 10 times the CDP-diacylglycerol of wild-type cells. Compensatory elevation of the mRNA level of other homologues was observed (in Delta ltaS cells the level of yfnI was elevated; in Delta yfnI cells that of yqgS and yvgJ was elevated; both were even higher in Delta ltaS Delta yfnI cella In Delta ltaS cells, the mRNA level of yfnI was corroborated to be regulated by sigma(M), which is activated in the null mutant cells. In Delta yfnI cells, the mRNA levels of yqgS and yvgJ reverted to less than those of wild-type when a defective sigX allele was introduced. Since sigX was activated in cells with Delta yfnI, this suggests that the induction of yqgS and yvgJ is dependent on sigma(X). The LTAs produced by the four ltaS homologues seem to play distinct physiological roles to maintain the full function of LTA on the B. subtilis cell envelope.
SOC GENERAL MICROBIOLOGY, English, Scientific journal
DOI：https://doi.org/10.1099/mic.0.063420-0
DOI ID：10.1099/mic.0.063420-0, ISSN：1350-0872, Web of Science ID：WOS:000315561000003 - Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli
Yasuhiro Shiba; Hiroyoshi Miyagawa; Hideki Nagahama; Kenji Matsumoto; Daitetsu Kondo; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
MICROBIOLOGY-SGM, Volume：158, Number：Pt 5, First page：1238, Last page：1248, May 2012, [Reviewed]
The Rcs phosphorelay signal transduction system controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. We investigated the activation of the Rcs system in a pgsA null mutant of Escherichia coli, which completely lacks the major acidic phospholipids phosphatidylglycerol and cardiolipin. We found that the Rcs activation, and consequent thermosensitivity, were suppressed by overexpression of the lgt gene, encoding diacylglyceryltransferase, which catalyses the modification of prolipoproteins that is the first step in the maturation and localization process of lipoproteins, and is a prerequisite for the later steps. The outer-membrane lipoprotein RcsF is an essential component of Rcs signalling. This lipoprotein was poorly localized to the outer membrane in the pgsA null mutant, probably because of the absence of phosphatidylglycerol, the major donor of diacylglycerol in the Lgt reaction. Even in a pgsA(+) background, the Rcs system was activated when RcsF was mislocalized to the inner membrane by alteration of the residues at positions 2 and 3 of its mature form to inner-membrane retention signals, or when it was mislocalized to the periplasm by fusing the mature form to maltose-binding protein. These results suggest that RcsF functions as a ligand for RcsC in activating Rcs signalling. Mislocalized versions of RcsF still responded to mutations pgsA, mdoH and tolB, further activating the Rcs system, although the rfaP mutation barely caused activation. It seems that RcsF must be localized in the outer membrane to respond effectively to stimuli from outside the cell.
SOC GENERAL MICROBIOLOGY, English, Scientific journal
DOI：https://doi.org/10.1099/mic.0.056945-0
DOI ID：10.1099/mic.0.056945-0, ISSN：1350-0872, PubMed ID：22322964, Web of Science ID：WOS:000305375900010 - Expression and Localization of Two SecA Homologs in the Unicellular Red Alga Cyanidioschyzon merolae
Yosuke Koyama; Yasuko Kaneko; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara; Niji Ohta
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：76, Number：2, First page：417, Last page：422, Feb. 2012, [Reviewed]
SecA is an ATP-driven motor for Sec translocase that participates in bacterial protein export and thylakoidal import in plants. We have reported that Cyanidioschyzon merolae, a unicellular red alga, possesses a nuclear-encoded secA(nuc) and a plastid-encoded secA (pt) gene. In this study we found that the amount of SecA(nuc) protein almost quadrupled at high temperature, whereas that of the SecA(pt) protein increased far less. We were also able to determine the localization of both SecAs to the chloroplast by immunofluorescence and immunoelectron microscopy. We suggest that SecA(nuc) has an important role in the chloroplast at high temperatures.
TAYLOR & FRANCIS LTD, English, Scientific journal
DOI：https://doi.org/10.1271/bbb.110833
DOI ID：10.1271/bbb.110833, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：22361818, Web of Science ID：WOS:000301092300041 - The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis
Satoshi Matsuoka; Michihiro Hashimoto; Yusuke Kamiya; Takeshi Miyazawa; Kazuki Ishikawa; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：365, Last page：376, Dec. 2011, [Reviewed], [Lead]
The dgkB gene is essential for the growth of Bacillus subtilis. It encodes a diacylglycerol (DG) kinase that converts DG to phosphatidic acid to reintroduce it into the phospholipid synthesis pathway. Repression of the dgkB gene placed under a regulatable promoter causes accumulation of DG and leads to lethality. DG is formed as a byproduct of the synthesis of lipoteichoic acid (LTA), a polyanionic component of the cell envelope. B. subtilis synthesizes LTA by polymerizing the glycerophosphate moiety of phosphatidylglycerol (PG) onto a glucolipid membrane anchor, and releasing the DG moiety of PG. B. subtilis has four genes homologous to Staphylococcus aureus ltaS, which encodes LTA synthase. Disruption of either or both of two genes, yflE and yfnI, whose products show higher homology with S. aureus LtaS among the four homologues, suppressed the lethality caused by dgkB repression. In cells with dgkB repression, DG was accumulated to 43 +/- 3% of total lipids, about three times the content of wild type cells (13 +/- 1%). Disruption of yfnI in the dgkB-repressed cells reduced the DG content to 15 +/- 2%, but yflE-disruption did not (42 +/- 1%); this was probably due to efficient LTA synthesis by YfnI in the yflE-disrupted cells. Further introduction of a disrupted allele of ugtP, encoding glucolipid synthase that consumes DG as a substrate, partially lowered the colony forming capacity in strains with yflE-disruption. A disrupted dgkB allele was successfully introduced into strains disrupted for either or both of yflE and yfnI, indicating that the essential gene dgkB is dispensable in mutants defective in LTA synthesis.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.86.365
DOI ID：10.1266/ggs.86.365, ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800002 - Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae
Yosuke Koyama; Koji Takimoto; Asuka Kojima; Kei Asai; Satoshi Matsuoka; Toshiaki Mitsui; Kouji Matsumoto; Hiroshi Hara; Niji Ohta
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：75, Number：10, First page：2073, Last page：2078, Oct. 2011, [Reviewed]
SecA is an ATP-driven motor for protein translocation in bacteria and plants. Mycobacteria and listeria were recently found to possess two functionally distinct secA genes. In this study, we found that Cyanidioschyzon merolae, a unicellular red alga, possessed two distinct secA-homologous genes; one encoded in the cell nucleus and the other in the plastid genome. We found that the plastid-encoded SecA homolog showed significant ATPase activity at low temperature, and that the ATPase activity of the nuclear-encoded SecA homolog showed significant activity at high temperature. We propose that the two SecA homologs play different roles in protein translocation.
TAYLOR & FRANCIS LTD, English, Scientific journal
DOI：https://doi.org/10.1271/bbb.110338
DOI ID：10.1271/bbb.110338, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：21979100, Web of Science ID：WOS:000296413300040 - Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids
Satoshi Matsuoka; Minako Chiba; Yu Tanimura; Michihiro Hashimoto; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：5, First page：295, Last page：304, Oct. 2011, [Reviewed], [Lead]
Bacillus subtilis Marburg 168 cells with disrupted ugtP, which encodes UDP-glucosyltransferase involved in glucolipid synthesis, were bent and distended. In the ugtP mutant cells, the extracytoplasmic function sigmas SigM, SigV and SigX, were found to be activated. Introduction of a disrupted allele of sigM into the ugtP strain caused even more abnormal morphology, with cells taking on a balloon-like shape; growth of these cells in LB medium was hampered by addition of 1.5% NaCl. Addition of MgSO4 or MnCl2 suppressed the abnormal morphology. In ugtP mutant cells the transcription of the mreB operon from an upstream promoter in maf (designated Pupstream mreB) and PmreBH was 4.3- and 2.3-fold higher, respectively, and localization of GFP-MreB was not in discrete dots (in an apparently helical pattern), but faint and in irregular clusters. GFP-MreB protein was reduced in the ugtP mutant cells. We suggest that glucolipids are important for MreB isoforms to take on the configuration that appears as discrete dots and plays a role in shaping cells into straight rods.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.86.295
DOI ID：10.1266/ggs.86.295, ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000302887400002 - The Genome of Bacillus subtilis Phage SP10: A Comparative Analysis with Phage SPO1
Lii Mien Yee; Takashi Matsumoto; Koichi Yano; Satoshi Matsuoka; Yoshito Sadaie; Hirofumi Yoshikawa; Kei Asai
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：75, Number：5, First page：944, Last page：952, May 2011, [Reviewed]
A nucleotide sequence of the whole genome of Bacillus subtilis phage SP10 was determined. It was composed of 143,986 bp with 236 putative open reading frames (ORFs). Sixty-five of 236 predicted ORFs showed high similarity to that of SPO1, and the genome organizations of the two phages were similar to each other. SP10 belongs to the Myoviridae family, for which the well-studied phage SPO1 is the representative phage. Hence, we compared SP10 to SPO1. The SP10 genome DNA showed different sensitivity to restriction enzymes than SPO1, due to differences in base modification. According to transcriptional analysis, the gene expression of regulatory network of SP10 was similar to SPO1. It was observed that RNA polymerase containing sigma-A was inactive in directing the host genes but active in directing the phage genes. It appeared that the association of sigma-A with the core enzyme complex of RNA polymerase was strengthened during development.
TAYLOR & FRANCIS LTD, English, Scientific journal
DOI：https://doi.org/10.1271/bbb.100921
DOI ID：10.1271/bbb.100921, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：21597187, Web of Science ID：WOS:000291519100021 - Heterologous Expression of the Oceanobacillus iheyensis SigW and Its Anti-Protein RsiW in Bacillus subtilis
Koichi Yano; Hiromi Inoue; Hirokazu Mori; Lii Mien Yee; Satoshi Matsuoka; Yoshito Sadaie; Kei Asai
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：75, Number：5, First page：966, Last page：975, May 2011, [Reviewed]
Pairs of the ECF sigma factor and its anti-sigma factor, SigW and RsiW, of Bacillus-related species that inhabit extreme environments were heterologously expressed in B. subtilis. All the RsiWs, membrane proteins, failed to fill their function of repressing cognate SigW activity, despite their close structural similarities. Particularly, uncontrolled expression of Oceanobacillus iheyensis OISigW due to abortive OIRsiW was harmful to B. subtilis. Analysis of revertants of this growth defect and site-directed mutagenesis indicated that the insertion of six and a minimum of three hydrophobic amino acid residues occurring in the transmembrane region allowed OIRsiW to function as anti-OISigW. Subcellular localization of OIRsiW was detected by immunoblot analysis, suggesting that both the wild-type and the mutant form of OIRsiW were localized to the membrane. An appropriate length of a transmembrane region required for proper integration into the membrane after translocation might vary among these Bacillus-related species.
TAYLOR & FRANCIS LTD, English, Scientific journal
DOI：https://doi.org/10.1271/bbb.110035
DOI ID：10.1271/bbb.110035, ISSN：0916-8451, eISSN：1347-6947, Web of Science ID：WOS:000291519100024 - Inhibitory effect of prophage SP beta fragments on phage SP10 ribonucleotide reductase function and its multiplication in Bacillus subtilis
Lii Mien Yee; Satoshi Matsuoka; Koichi Yano; Yoshito Sadaie; Kei Asai
GENES & GENETIC SYSTEMS, Volume：86, Number：1, First page：7, Last page：18, Feb. 2011, [Reviewed]
Bacteria have evolved various kinds of defense mechanisms against phage infection and multiplication. Analysis of these mechanisms is important for medical and industrial application of phages as well as for their scientific study. Strains of Bacillus subtilis Marburg strain carrying both nonA and nonB mutations are susceptible to the Bacillus phage SP10. The nonB mutation has been shown to have a compromised intrinsic restriction system. The nonA mutation represents the cured state of prophage SP beta whose genome is 135 kb in length and contains 187 ORFs. For this study we investigated the molecular mechanism behind the inhibitory activity of the wild type nonA function against phage SP10 development. The progression of phage-developmental stages was examined in cells harboring wild type nonA, i.e. prophage SP beta. After phage adsorption and DNA injection into host cells, the synthesis of phage specific mRNA proceeded normally. However, phage DNA synthesis was severely inhibited by some effect of wild type nonA. We thus systematically deleted portions of the prophage SP beta region from the B. subtilis genome and the resultant mutant strains were examined as to whether they still retained sufficient wild type nonA functionality to inhibit SP10 phage development. The SP beta region encompassing the bnrdEF gene, which codes for a putative ribonucleotide reductase (RRase), turned out to be responsible for the wild type nonA function. The phage SP10 possesses its own xnrdE gene coding for a putative RRase that complements the temperature-sensitive mutation of the host RRase gene nrdE. This complementation was blocked by an artificially induced transcription from a non-coding strand of the bnrdEF region. It is thus likely that the transcript from the bnrdEF region of SP beta inhibits ribonucleotide reductase function of SP10, resulting in arrest of DNA synthesis during phage SP10 development.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.86.7
DOI ID：10.1266/ggs.86.7, ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000292010900002 - Involvement of sigma(S) accumulation in repression of the flhDC operon in acidic phospholipid-deficient mutants of Escherichia coli
Junji Uchiyama; Yuka Nobue; Hong Zhao; Hiroshi Matsuzaki; Hideki Nagahama; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
MICROBIOLOGY-SGM, Volume：156, Number：Pt 6, First page：1650, Last page：1660, Jun. 2010, [Reviewed]
Escherichia coli pgsA mutations, which cause acidic phospholipid deficiency, repress transcription of the flagellar master operon flhDC, and thus impair flagellar formation and motility. The molecular mechanism of the strong repression of flhDC transcription in the mutant cells, however, has not yet been clarified. In order to shed light on this mechanism we isolated genes which, when supplied in multicopy, suppress the repression of flhD, and found that three genes, gadW, metE and yeaB, were capable of suppression. Taking into account a previous report that gadW represses sigma(S) production, the level of sigma(S) in the pgsA3 mutant was examined. We found that pgsA3 cells had a high level of sigma(S) and that introduction of a gadW plasmid into pgsA3 cells did reduce the sigma(S) level. The pgsA3 cells exhibited a sharp increase in sigma(S) levels that can only be partially attributed to the slight increase in rpoS transcription; the largest part of the effect is due to a post-transcriptional accumulation of sigma(S). GadW in multicopy exerts its effect by post-transcriptionally downregulating sigma(S). YeaB and MetE in multicopy also exert their effect via sigma(S). Disruption of rpoS caused an increase of the flhD mRNA level, and induction from P-trc-rpoS repressed the flhD mRNA level. The strong repression of flhD transcription in pgsA3 mutant cells is thus suggested to be caused by the accumulated sigma(S).
SOC GENERAL MICROBIOLOGY, English, Scientific journal
DOI：https://doi.org/10.1099/mic.0.036749-0
DOI ID：10.1099/mic.0.036749-0, ISSN：1350-0872, PubMed ID：20185506, Web of Science ID：WOS:000279798900008 - Accumulation of Sigma S due to enhanced synthesis and decreased degradation in acidic phospholipid-deficient Escherichia coli cells
Junji Uchiyama; Yu Sasaki; Hideki Nagahama; Aya Itou; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
FEMS MICROBIOLOGY LETTERS, Volume：307, Number：2, First page：120, Last page：127, Jun. 2010, [Reviewed]
The Escherichia coli pgsA3 mutation, which causes deficiency in acidic phospholipids, leads to a significant accumulation of Sigma S. This accumulation is partly accounted for by the higher transcription level of rpoS; however, it has also been suggested that the cells accumulate Sigma S post-transcriptionally. We found that the level of the small regulatory RNA RprA, which is involved in the promotion of rpoS translation, is higher in pgsA3 cells than in pgsA+ cells. Induction of altered rpoS mRNA that does not depend on RprA in pgsA+ cells did not increase the level of Sigma S to the high level observed in pgsA3 cells, suggesting post-translational Sigma S accumulation in the latter. The mRNA levels of clpX and clpP, whose products form a ClpXP protease that degrades Sigma S, were much reduced in pgsA3 cells. Consistent with the reduced mRNA levels, the half-life of Sigma S in pgsA3 cells was much longer than in pgsA+ cells, indicating that downregulation of the degradation is a major cause for the high Sigma S content. We show that the downregulation can be partially attributed to activated CpxAR in the mutant cells, which causes repression of rpoE on whose gene product the expression of clpPX depends.
WILEY-BLACKWELL, English, Scientific journal
DOI：https://doi.org/10.1111/j.1574-6968.2010.01964.x
DOI ID：10.1111/j.1574-6968.2010.01964.x, ISSN：0378-1097, PubMed ID：20455949, Web of Science ID：WOS:000277794500003 - Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis
Yoshinori Hara; Masahide Seki; Satoshi Matsuoka; Hiroshi Hara; Atsushi Yamashita; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：433, Last page：442, Dec. 2008, [Reviewed]
The gene responsible for the first acylation of sn-glycerol-3-phosphate (G3P) in Bacillus subtilis has not yet been determined with certainty. The product of this first acylation, lysophosphatidic acid (LPA), is subsequently acylated again to form phosphatidic acid (PA), the primary precursor to membrane glycerolipids. A novel G3P acyltransferase (GPAT), the gene product of plsY, which uses acylphosphate formed by the plsX gene product, has recently been found to synthesize LPA in Streptococcus pneumoniae. We found that in B. subtilis growth arrests after repression of either a plsY homologue or a plsX homologue were overcome by expression of E. coli plsB, which encodes an acyl-acylcarrier protein (acyl-ACP)dependent GPAT, although in the case of plsX repression a high level of plsB expression was required. B. subtilis has, therefore, a capability to use the acyl-ACP dependent GPAT of PIsB. Simultaneous expression of plsY and plsX suppressed the glycerol requirement of a strict glycerol auxotrophic derivative of the E. coli plsB26 mutant, although either one alone did not. Membrane fractions from B. subtilis cells catalyzed palmitoylphosphate-dependent acylation of [C-14]- labeled G3P to synthesize [C-14] -labeled LPA, whereas those from Delta plsY cells did not. The results indicate unequivocally that PIsY is an acyl-phosphate dependent GPAT. Expression of plsX corrected the glycerol auxotrophy of a Delta ygiH (the deleted allele of an E. coli homologue of plsY) derivative of BB26-36 (plsB26 plsX50), suggesting an essential role of plsX other than substrate supply for acylphosphate dependent LPA synthesis. Two-hybrid examinations suggested that PIsY is associated with PlsX and that each may exist in multimeric form.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.83.433
DOI ID：10.1266/ggs.83.433, ISSN：1341-7568, eISSN：1880-5779, CiNii Articles ID：10024791035, PubMed ID：19282621, SCOPUS ID：63849202975, Web of Science ID：WOS:000265228400001 - Effect of multiple copies of cohesins on cellulase and hemicellulase activities of Clostridium cellulovorans mini-cellulosomes
Jaeho Cha; Satoshi Matsuoka; Helen Chan; Hideaki Yukawa; Masayuki Inui; Roy H. Doi
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Volume：17, Number：11, First page：1782, Last page：1788, Nov. 2007, [Reviewed]
Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components. In this study, we determined the synergistic effects on cellulosic and hemicellulosic substrates by three different recombinant mini-cellulosomes containing either endoglucanase EngB or endoxylanase XynA bound to mini-CbpA with one cohesin domain (mini-CbpA1), two cohesins (mini-CbpA12), or four cohesins (mini-CbpA1234). The assembly of EngB or XynA with mini-CbpA increased the activity against carboxymethyl cellulose, acid-swollen cellulose, Avicel, xylan, and corn fiber 1.1-1.8-fold compared with that for the corresponding enzyme alone. A most distinct improvement was shown with corn fiber, a natural substrate containing xylan, arabinan, and cellulose. However, there was little difference in activity between the three different mini-cellulosomes when the cellulosomal enzyme concentration was held constant regardless of the copy number of cohesins in the cellulosome. A synergistic effect was observed when the enzyme concentration was increased to be proportional to the number of cohesins in the mini-cellulosome. The highest degree of synergy was observed with mini-CbpA1234 (1.8-fold) and then mini-CbpA12 (1.3-fold), and the lowest synergy was observed with mini-CbpA1 (1.2-fold) when Avicel was used as the substrate. As the copy number of cohesin was increased, there was more synergy. These results indicate that the clustering effect (physical enzyme proximity) of the enzyme within the mini-cellulosome is one of the important factors for efficient degradation of plant cell walls.
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY, English, Scientific journal
ISSN：1017-7825, eISSN：1738-8872, Web of Science ID：WOS:000251242500006 - Synergistic interaction of Clostridium cellulovorans cellulosomal cellulases and HbpA
Satoshi Matsuoka; Hideaki Yukawa; Masayuki Inui; Roy H. Doi
JOURNAL OF BACTERIOLOGY, Volume：189, Number：20, First page：7190, Last page：7194, Oct. 2007, [Reviewed], [Lead]
Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellulosomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL). Synergistic degradative action of the cellulosomal cellulase and HbpA complexes was demonstrated on acid-swollen cellulose, Avicel, and corn fiber. We propose that HbpA functions to bind dockerin-containing cellulosomal enzymes to the cell surface and complements the activity of cellulosomes.
AMER SOC MICROBIOLOGY, English, Scientific journal
DOI：https://doi.org/10.1128/JB.00842-07
DOI ID：10.1128/JB.00842-07, ISSN：0021-9193, eISSN：1098-5530, CiNii Articles ID：80018160912, PubMed ID：17693494, SCOPUS ID：35048871939, Web of Science ID：WOS:000250100000004 - Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation
Takamitsu Arai; Satoshi Matsuoka; Hee-Yeon Cho; Hideaki Yukawa; Masayuki Inui; Sui-Lam Wong; Roy H. Doi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：104, Number：5, First page：1456, Last page：1460, Jan. 2007, [Reviewed]
The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB were cocultured, minicellulosomes were synthesized, consisting in one case of miniCbpA and EngB and in the second case of miniCbpA and XynB. Both minicellulosomes showed their respective enzymatic activities. We call this phenomenon "intercellular complementation." Interesting implications concerning bacterial cooperation are suggested from these results.
NATL ACAD SCIENCES, English, Scientific journal
DOI：https://doi.org/10.1073/pnas.0610740104
DOI ID：10.1073/pnas.0610740104, ISSN：0027-8424, CiNii Articles ID：80018655406, PubMed ID：17244702, SCOPUS ID：33846818917, Web of Science ID：WOS:000244081000005 - Restriction and modification of SP10 phage by BsuM of Bacillus subtilis Marburg
S Matsuoka; K Asai; Y Sadaie
FEMS MICROBIOLOGY LETTERS, Volume：244, Number：2, First page：335, Last page：339, Mar. 2005, [Reviewed], [Lead]
Bacillus subtilis Marburg has only one intrinsic restriction and modification system BsuM that recognizes the CTCGAG (XhoI site) sequence. It consists of two operons, BsuMM operon for two cytosine DNA methyltransferases, and BsuMR operon for a restriction nuclease and two associated proteins of unknown function. In this communication, we analyzed the BsuM system by utilizing phage SPIO that possesses more than twenty BsuM target sequences on the phage genome. SPIO phages grown in the restriction and modification-deficient strain could not make plaques on the restriction-proficient BsuMR(+) indicator strain. An enforced expression of the wild type BsuMM operon in the BsuMR(+) indicator strain, however, allowed more than thousand times more plaques. DNA extracted from SPIO phages, thus, propagated became more but not completely refractory to XhoI digestion in vitro. Thus, the SPIO phage genome DNA is able to be nearly full-methylated but some BsuM sites are considered to be unmethylated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, English, Scientific journal
DOI：https://doi.org/10.1016/j.femsle.2005.02.006
DOI ID：10.1016/j.femsle.2005.02.006, ISSN：0378-1097, PubMed ID：15766787, Web of Science ID：WOS:000227860400015 - Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phage
S Matsuoka; T Arai; R Murayama; F Kawamura; K Asai; Y Sadaie
GENES & GENETIC SYSTEMS, Volume：79, Number：6, First page：311, Last page：317, Dec. 2004, [Reviewed], [Lead]
Mutational inactivation of both nonA and nonB genes are required for the permissiveness of Bacillus subtilis Marburg cells to infection by phage SPIO. By transformational analysis of the nonA strain with DNAs from gently lysed protoplasts carrying the integrative plasmid pMUTIN (em) insertions in every 20 kb along the whole chromosome, we have identified the nonA to be the cured state of endogenous prophage SP beta. Direct DNA sequencing, on the other hand, revealed one nonsense mutation of nonB in ydiR, which is a component gene of the intrinsic restriction system BsuMR of B.subtilis Marburg. Introduction of the wild type ydiR into the nonB strain at aprE locus resulted in complementation of nonB. Furthermore, as the SP10 genome was found to possess multiple BsuM target sites, it is considered that SP10 can infect and multiply in B.subtilis cells, which are SP beta free and possess a defective BsuMR restriction system.
GENETICS SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.1266/ggs.79.311
DOI ID：10.1266/ggs.79.311, ISSN：1341-7568, eISSN：1880-5779, PubMed ID：15728999, Web of Science ID：WOS:000228241800001 - Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg
H Ohshima; S Matsuoka; K Asai; Y Sadaie
JOURNAL OF BACTERIOLOGY, Volume：184, Number：2, First page：381, Last page：389, Jan. 2002, [Reviewed]
Transcriptional analysis and disruption of five open reading frames (ORFs), ydiO, ydiP, ydiR, ydiS, and ydjA, in the prophage 3 region of the chromosome of Bacillus subtilis Marburg revealed that they are component genes of the intrinsic BsuM restriction and modification system of this organism. The classical mutant strain RM125, which lacks the restriction and modification system of B. subtilis Marburg, lacks the prophage 3 region carrying these five Offs. These ORFs constitute two operons, the ydiO-ydiP operon and the ydiR-ydiS-ydjA operon, both of which are expressed during the logarithmic phase of growth. The predicted gene products YdiO and YdiP are the orthologues of cytosine DNA methyltransferases. The predicted YdiS product is an orthologue of restriction nucleases, while the predicted YdiR and YdjA products have no apparent paralogues and orthologues whose functions are known. Disruption of the ydiR-ydiS-ydjA operon resulted in enhanced transformation by plasmid DNA carrying multiple BsuM target sequences. Disruption of ydiO or ydiP function requires disruption of at least one of the following genes on the chromosome: ydiR, ydiS, and ydjA. The degrees of methylation of the BsuM target sequences on chromosomal DNAs were estimated indirectly by determining the susceptibility to digestion with XgoI (an isoschizomer of BsuM) of DNAs extracted from the disruptant strains. Six XhoI (BsuM) sites were examined. XhoI digested at the XhoI sites in the DNAs from disruptants with disruptions in both operons, while XhoI did not digest at the XhoI sites in the DNAs from the wild-type strain or from the disruptants with disruptions in the ydiR-ydiS-ydjA operon. Therefore, the ydiO-ydiP operon and the ydiR-ydiS-ydjA operon are considered operons that are responsible for BsuM modification and BsuM restriction, respectively.
AMER SOC MICROBIOLOGY, English, Scientific journal
DOI：https://doi.org/10.1128/JB.184.2.381-389.2002
DOI ID：10.1128/JB.184.2.381-389.2002, ISSN：0021-9193, PubMed ID：11751814, Web of Science ID：WOS:000173009700006
- Physiological function of membrane lipids and cell surface structure in bacteria
Satoshi Matsuoka
Genes and Genetic Systems, Volume：92, Number：5, First page：215, 2017
Genetics Society of Japan, English, Book review
DOI：https://doi.org/10.1266/ggs.92.215
DOI ID：10.1266/ggs.92.215, ISSN：1880-5779, SCOPUS ID：85045196914 - Septal localization by membrane targeting sequences at the COOH terminus of Bacillus subtilis cardiolipin synthase
Yukiko Imai; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：380, Last page：380, Dec. 2016, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000245 - An attempt to search B.subtilis gene that rescues lethality of E.coli strain lacking PE/CL
Michimune Ueno; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：380, Last page：380, Dec. 2016, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000244 - Molecular mechanism of regulation of two component system WaIKR by glucolipids in Bacillus subtilis
Satoshi Matsuoka; Kouji Matsumoto; Kei Asai; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：350, Last page：350, Dec. 2016, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000127 - Analysis of the activation mechanism of an extracytoplasmic function sigma factor sigma V in the Bacillus subtilis mutant cells lacking glucolipids by using chimera-antisigma factors
Takahiro Seki; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：350, Last page：350, Dec. 2016, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000126 - Visualization of protein-protein interactions among Rcs components in Escherichia coli by Periplasmic BiFC
Takatsugu Sato; Akira Takano; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：350, Last page：350, Dec. 2016
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000129 - Function of E.coli RcsF in activation of Rcs signal transduction system
Tomoko Izawa; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：350, Last page：350, Dec. 2016
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000128 - Analysis of transcriptional regulation of sigI in Bacillus subtilis
Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：90, Number：6, First page：382, Last page：382, Dec. 2015, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000377556400090 - Interaction between RcsF and RcsC in the periplasmic space of Escherichia coli
Takatsugu Sato; Akira Takano; Koji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：90, Number：6, First page：399, Last page：399, Dec. 2015
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000377556400156 - An attempt to replace major membrane lipids by monoglucosyldiacylglycerol in Escherichia coli
Michimune Ueno; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：90, Number：6, First page：400, Last page：400, Dec. 2015
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000377556400161 - Analysis of E.coli RcsF, a component of the Rcs signal transduction system
Tomoko Izawa; Akira Takano; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：90, Number：6, First page：399, Last page：399, Dec. 2015
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000377556400157 - The role of glucolipids in the regulation of ECF sigma factors in Bacillus subtilis
Takahiro Seki; Ryota Mineshima; Michihiro Hashimoto; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：89, Number：6, First page：330, Last page：330, Dec. 2014, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000357360600208 - Influence of glucolipids on regulation of SigI in Bacillus subtilis
Satoshi Matsuoka; Kaori Nobe; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：89, Number：6, First page：322, Last page：322, Dec. 2014, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000357360600177 - Analysis of interaction between RcsF and periplasmic region of RcsC of the Rcs phosphorelay signal transduction system of Echerichia coli
Akira Takano; Yasuhiro Shiba; Hiroyoshi Miyagawa; Mitsuru Umekawa; Kouji Matsumoto; Satoshi Matsuoka; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：89, Number：6, First page：329, Last page：329, Dec. 2014
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000357360600207 - Role of glycolipid in maintenance of cell morphology in Bacillus subtilis
Satoshi Matsuoka; Midori Kamimura; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：88, Number：6, First page：390, Last page：390, Dec. 2013, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000337552900229 - Mechanism of Localization of Bacillus subtilis MinD by analysis on the interaction with acidic phospholipids
Yugo Furukawa; Erika Wada; Nobuaki Karasawa; Wakana Matsushima; Kazuki Ishikawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：88, Number：6, First page：387, Last page：387, Dec. 2013, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000337552900217 - Functional Analysis of the Basic Regions at N and C termini of Phosphatidylserine Synthase of Escherichia coli
Yuki Nishino; Yutaka Furukawa; Daiki Umedu; Kuniko Ochiai; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara
GENES & GENETIC SYSTEMS, Volume：88, Number：6, First page：390, Last page：390, Dec. 2013
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000337552900228 - 枯草菌における糖脂質欠損、ホスファチジルグリセロール減少、リポテイコ酸欠損によるECFシグマ因子の活性化
関 貴洋; 橋本 理尋; 松岡 聡; 松本 幸次; 原 弘志
Volume：55, First page：179, Last page：182, 28 May 2013
Japanese
ISSN：0285-1520, CiNii Articles ID：10031176701, CiNii Books ID：AN00102325 - Analysis of the function of UgtP involved in maintenance of cell morphology in Bacillus subtilis
Yuki Nishino; Saori Miyamatsu; Minako Chiba; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：434, Last page：434, Dec. 2012, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700244 - Induction of extracytoplasmic function sigma factors in Bacillus subtilis cells with defective lipoteichoic acid
Michihiro Hashimoto; Takahiro Seki; Satoshi Matsuoka; Hiroshi Hara; Kei Asai; Yoshito Sadaie; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：433, Last page：433, Dec. 2012, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700241 - Analysis of the role of two amphipathic alpha-helices at the C-terminus of Bacillus subtilis MinD
Yugo Furukawa; Nobuaki Karasawa; Wakana Matsushima; Kazuki Ishikawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：434, Last page：434, Dec. 2012, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700243 - Analysis of induction of extracytoplasmic function sigma factors in Bacillus subtilis cells lacking glucolipids
Takahiro Seki; Michihiro Hashimoto; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：433, Last page：433, Dec. 2012, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700240 - Analysis of sigma(S) accumulation mechanism in acidic phospholipid-deficient Escherichia coli cells
Tatsumi Oikawa; Eri Kimura; Yu Sasaki; Junji Uchiyama; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：420, Last page：420, Dec. 2012
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700188 - Influence of Glucolipids on Regulation of Extracytoplasmic Function Sigma Factors
Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：87, Number：6, First page：419, Last page：419, Dec. 2012
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000323535700187 - ストレス応答ECFシグマ因子を介した枯草菌におけるリポテイコ酸の恒常性の維持
橋本 理尋; 関 貴洋; 松岡 聡; 原 弘志; 朝井 計; 定家 義人; 松本 幸次
Volume：54, First page：138, Last page：141, 28 May 2012
Japanese
ISSN：0285-1520, CiNii Articles ID：10030581402, CiNii Books ID：AN00102325 - Examination of the function of UgtP in maintenance of cell morphology in Bacillus subtilis
Saori Miyamatsu; Minako Chiba; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：432, Last page：432, Dec. 2011, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800197 - Complementation of abnormal morphology of Bacillus subtilis glucolipid-lacking cells by introduction of heterologous glycolipid synthases
Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：432, Last page：432, Dec. 2011, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
DOI：https://doi.org/10.1266/ggs.86.295
DOI ID：10.1266/ggs.86.295, ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800198 - Analysis of the mechanism of the septal localization of MinD in Bacillus subtilis cells
Wakana Matsushima; Kazuki Ishikawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：431, Last page：431, Dec. 2011, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800196 - Induction of ECF sigma factors and reduction of housekeeping sigma factor caused by the reduction of negative charge on cell surface in Bacillus subtilis
Michihiro Hashimoto; Yu Tanimura; Yusuke Kamiya; Satoshi Matsuoka; Hiroshi Hara; Kei Asai; Yoshito Sadaie; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：398, Last page：398, Dec. 2011, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800067 - Analysis of sigma(S) accumulation in acidic phospholipid-deficient Escherichia coli cells
Tatsumi Oikawa; Yu Sasaki; Junji Utiyama; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：409, Last page：409, Dec. 2011
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800108 - Functional analysis and physiological significance of two SecA ATPases in Cyanidioschyzon merolae
Yosuke Koyama; Asuka Kojima; Aiko Oguro; Kouji Takimoto; Satoshi Matsuoka; Kei Asai; Kouji Matsumoto; Hiroshi Hara; Niji Ohta
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：411, Last page：411, Dec. 2011
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800118 - Analysis of synergistic effect between HbpA and cellurases in Clostridium cellulovorans
Ryota Mineshima; Ayumi Oana; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：86, Number：6, First page：408, Last page：408, Dec. 2011
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000303700800106 - 枯草菌膜脂質組成異常及びリポテイコ酸欠損によるストレス応答ECFシグマ因子の活性化
橋本 理尋; 松岡 聡; 原 弘志; 朝井 計; 定家 義人; 松本 幸次
Volume：53, First page：34, Last page：37, 28 Apr. 2011
Japanese
ISSN：0285-1520, CiNii Articles ID：10028178778, CiNii Books ID：AN00102325 - リポテイコ酸欠損細胞におけるECFシグマ因子の活性化
橋本 理尋; 谷村 遊; 神谷 雄介; 松岡 聡; 原 弘志; 朝井 計; 定家 義人; 松本 幸次
Volume：85, Number：5, First page：7, Last page：7, 2011, [Reviewed], [Invited]
Japanese, Introduction scientific journal - Essential role of diacylglycerol kinase DgkB in Bacillus subtilis
Yusuke Kamiya; Takeshi Miyazawa; Kazuki Ishikawa; Michihiro Hashimoto; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：412, Last page：412, Dec. 2010, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200065 - Analysis of the mechanism of the septal localization of MinD in B. subtilis
Kazuki Ishikawa; Junji Uchiyama; Wakana Matsushima; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：405, Last page：405, Dec. 2010, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200038 - Influence of glucolipid-deficiency on cell morphology of Bacillus subtilis
Minako Chiba; Yu Tanimura; Satoshi Matsuoka; Ke Asai; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：405, Last page：405, Dec. 2010, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200037 - Analysis of biological function of glucolipids in Bacillus subtilis
Satoshi Matsuoka; Minako Chiba; Yu Tanimura; Aya Taniguchi; Ryuichi Hiatari; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：404, Last page：404, Dec. 2010, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200036 - Analysis of functional domains in a proline-rich region of an outer membrane lipoprotein RcsF regulating the two-component regulatory system
Mitsuru Umekawa; Tatsurou Nakamura; Hiroyoshi Miyagawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：425, Last page：425, Dec. 2010
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200117 - Functional analysis of two protein-translocation motor SecAs in unicellular red alga Cyanidioschyzon merolae; one encoded in the cell nucleus and the other in the plastid genome
Yosuke Koyama; Koji Takimoto; Asuka Kojima; Kei Asai; Satoshi Matsuoka; Kouji Matsumoto; Hiroshi Hara; Niji Ohta
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：398, Last page：398, Dec. 2010
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200011 - Induction of ECF sigma factors in the cells with a defect of lipoteichoic acid
Michihiro Hashimoto; Yui Tanimura; Yusuke Kamiya; Satoshi Matsuoka; Hiroshi Hara; Kei Asai; Yoshito Sadaie; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：85, Number：6, First page：424, Last page：424, Dec. 2010
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000290243200113 - 枯草菌ジアシルグリセロールキナーゼ遺伝子dgkBは必須遺伝子だが, リポテイコ酸合成欠損株では挿入破壊可能となる
原 弘志; 神谷 雄介; 宮澤 岳; 石川 一輝; 橋本 理尋; 松岡 聡; 松本 幸次
Volume：52, First page：48, Last page：51, 25 May 2010
Japanese
ISSN：0285-1520, CiNii Articles ID：10026406993, CiNii Books ID：AN00102325 - 枯草菌細胞分裂位置決定機構への膜酸性リン脂質の寄与
石川 一輝; 松岡 聡; 原 弘志; 松本 幸次
Volume：52, First page：145, Last page：148, 25 May 2010
Japanese
ISSN：0285-1520, CiNii Articles ID：10026407142, CiNii Books ID：AN00102325 - 枯草菌膜脂質組成及び細胞表層変化に伴うストレス応答ECFシグマ因子の活性化
橋本 理尋; 谷村 遊; 神谷 雄介; 松岡 聡; 原 弘志; 朝井 計; 定家 義人; 松本 幸次
Volume：52, First page：149, Last page：152, 25 May 2010
Japanese
ISSN：0285-1520, CiNii Articles ID：10026407156, CiNii Books ID：AN00102325 - 細菌のホスファチジン酸合成に関与するアシルトランスフェラーゼの多様性 : アシルリン酸をアシルドナーとする新規アシルトランスフェラーゼの発見から
原 義令; 松岡 聡; 原 弘志; 山下 純; 松本 幸次
Volume：48, Number：5, First page：301, Last page：304, 01 May 2010
Japanese
DOI：https://doi.org/10.1271/kagakutoseibutsu.48.301
DOI ID：10.1271/kagakutoseibutsu.48.301, ISSN：0453-073X, CiNii Articles ID：10026438265, CiNii Books ID：AN00037573 - Analysis of glucolipid functions in Bacillus subtilis
Minako Chiba; Yu Tanimura; Satoshi Matsuoka; Kei Asai; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：456, Last page：456, Dec. 2009, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300097 - Dispensability of diacylglycerol kinase in Bacillus subtilis cells deficient in lipoteichoic acid biosynthesis
Yusuke Kamiya; Michihiro Hashimoto; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：456, Last page：456, Dec. 2009, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300096 - Molecular genetic studies of bacterial acyltransferases
Yoshinori Hara; Satoshi Matsuoka; Hiroshi Hara; Atsushi Yamashita; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：476, Last page：476, Dec. 2009
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300173 - A proline-rich region of the outer membrane lipoprotein RcsF plays a key role in regulating the Rcs signal transduction system in Escherichia coli
Mitsuru Umekawa; Hiriyoshi Miyagawa; Yasuhiro Shiba; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：450, Last page：450, Dec. 2009
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300072 - Analysis of interaction of an outer membrane lipoprotein Rcsf essential for activation of the Rcs phosphorelay system with inner membrane components in Escherichia coli
Hirofumi Okutsu; Daitetsu Kondou; Kenzi Matsumoto; Hiroyoshi Miyagawa; Yasuhiro Shiba; Satoshi Matsuoka; Hiroshi Hara; Kouzi Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：451, Last page：451, Dec. 2009
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300074 - The C-terminal domain of an outer membrane lipoprotein RcsF of E.coli functions in activating the Rcs phosphorelay signal transduction system
Daitetsu Kondo; Hiroyoshi Miyagawa; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：450, Last page：450, Dec. 2009
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300073 - acyl-phosphate をアシルドナーとする新規 glycerol-3-phosphate acyltransferase である枯草菌PlsYの機能解析
原 義令; 山下 純; 松岡 聡; 原 弘志; 松本 幸次
Volume：51, First page：61, Last page：64, 10 Jul. 2009
Japanese
ISSN：0285-1520, CiNii Articles ID：10025570063 - 枯草菌膜脂質組成変化に伴うストレス応答ECFシグマ因子の活性化
橋本 理尋; 谷村 遊; 神谷 雄介; 松岡 聡; 原 弘志; 朝井 計; 定家 義人; 松本 幸次
Volume：51, First page：274, Last page：277, 10 Jul. 2009
Japanese
ISSN：0285-1520, CiNii Articles ID：10025570324 - Activation of ECF sigma factor sigma(M) in Bacillus subtilis cells with altered membrane lipid composition
Tanimura Yu; Hashimoto Michihiro; Matsuoka Satoshi; Hara Hiroshi; Asai Kei; Matsumoto Kouji
GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：508, Last page：508, Dec. 2008
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000265228400121 - Analysis of functional domains of an outer membrane lipoprotein RcsF in regulation of the Rcs phosphorelay signal transduction system of Escherichia coli
Kondo Daitetsu; Miyagawa Hiroyosi; Matsuoka Satoshi; Hara Hiroshi; Matsumoto Kouji
GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：506, Last page：506, Dec. 2008
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000265228400115 - A transcriptional regulation of plural plant polysaccharide degrading enzyme gene clusters by Bacillus subtilis GmuR
Yee Liimien; Matsuoka Satoshi; Fujita Yasutaro; Asai Kei
GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：508, Last page：508, Dec. 2008, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000265228400122 - The resistance mechanism of Bacillus Subtilis to bacteriophage SPIO
Leilei Tao; Satoshi Matsuoka; Lii Mien Yee; Kei Asai
GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：482, Last page：482, Dec. 2008, [Reviewed]
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000265228400020 - Identification and analysis of nonA genes, which affect the propagation of bacteriophage SP10 in Bacillus Subtilis
Liimien Yee; Satoshi Matsuoka; Kei Asai; Yoshito Sadaie
GENES & GENETIC SYSTEMS, Volume：82, Number：6, First page：519, Last page：519, Dec. 2007
GENETICS SOC JAPAN, English, Summary international conference
ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000254238200090 - カリフォルニアでのセルロソーム研究
松岡 聡
Volume：45, Number：5, First page：364, Last page：367, 01 May 2007
Japanese
ISSN：0453-073X, CiNii Articles ID：10020174159, CiNii Books ID：AN00037573 - Structure, function, and application of cellulolytic complex "cellulosome" of Clostridium cellulovorans
DOI Roy H; MATSUOKA Satoshi
Bioscience & industry, Volume：65, Number：3, First page：121, Last page：5, 01 Mar. 2007
バイオインダストリー協会, Japanese
ISSN：0914-8981, CiNii Articles ID：10018852555, CiNii Books ID：AN10028025 - 海外だより「カリフォルニアでのセルロソーム研究」
松岡聡
Volume：45, First page：364, Last page：7, 2007 - 海外だより「カリフォルニアでのセルロソーム研究」
松岡聡
化学と生物, Volume：45, First page：364, Last page：7, 2007 - セルラーゼ複合体“セルロソーム”の構造・機能と利用
Roy Doi; 松岡 聡
バイオサイエンスとインダストリー, Volume：65, First page：121, Last page：5, 2007
■ Lectures, oral presentations, etc.
- 枯草菌糖脂質合成酵素遺伝子ugtPの機能解析
2009 - 大腸菌酸性リン脂質欠損におけるσS分解抑制の解析
2009 - 枯草菌膜脂質の組成変化によるECFσ因子の活性化
2009 - 枯草菌糖脂質の生理機能解析
2009 - 枯草菌カルジオリピン合成酵素の局在機構の解析
2009 - 細菌における脂質合成初期過程の分子遺伝学的研究
2009 - 大腸菌外膜リポタンパク質RcsFが担うRcsリン酸リレーシグナル伝達系活性化における機能的役割
2009 - バクテリオファージSP10の増殖に関するnonA遺伝子の解析
2009 - 枯草菌糖脂質合成酵素遺伝子ugtPの機能解析
2009 - 大腸菌酸性リン脂質欠損におけるσS分解抑制の解析
2009 - 枯草菌膜脂質の組成変化によるECFσ因子の活性化
2009 - 枯草菌糖脂質の生理機能解析
2009 - 枯草菌カルジオリピン合成酵素の局在機構の解析
2009 - 細菌における脂質合成初期過程の分子遺伝学的研究
2009 - 大腸菌外膜リポタンパク質RcsFが担うRcsリン酸リレーシグナル伝達系活性化における機能的役割
2009 - バクテリオファージSP10の増殖に関するnonA遺伝子の解析
2009 - リン酸リレーシグナル伝達系を介した大腸菌酸性リン脂質欠損におけるσSの蓄積機構の解析
2008 - SP10ファージに対する枯草菌の感染防御機構
2008 - 枯草菌膜脂質組成変化に伴うECFシグマ因子σMの活性化
2008 - 大腸菌Rcsリン酸リレーシグナル伝達系の制御に働く外膜リポタンパク質RcsF機能領域の解析
2008 - 枯草菌GmuRによる複数の植物性多糖分解酵素遺伝子群の転写制御
2008 - 枯草菌を用いたClostridium cellulovoransのミニセルロソームの構築
2008 - バクテリオファージSP10の増殖に関するnonA遺伝子の解析
2008 - リン酸リレーシグナル伝達系を介した大腸菌酸性リン脂質欠損におけるσSの蓄積機構の解析
2008 - SP10ファージに対する枯草菌の感染防御機構
2008 - 枯草菌膜脂質組成変化に伴うECFシグマ因子σMの活性化
2008 - 大腸菌Rcsリン酸リレーシグナル伝達系の制御に働く外膜リポタンパク質RcsF機能領域の解析
2008 - 枯草菌GmuRによる複数の植物性多糖分解酵素遺伝子群の転写制御
2008 - 枯草菌を用いたClostridium cellulovoransのミニセルロソームの構築
2008 - バクテリオファージSP10の増殖に関するnonA遺伝子の解析
2008
Poster presentation - Construction of Minicellulosome from Clostridium cellulovorans in Bacillus subtilis WB800
2007
Poster presentation - Expression of Cellulosomal Genes from Clostridium cellulovorans in Bacillus subtilis WB800 and Secretion of Their Products
2007
Poster presentation - Effect of Multiple Copies of Cohesins on Cellulase and Hemicellulase Activities of Clostridium cellulovorans Mini-Cellulosomes
2007
Poster presentation - Construction of Minicellulosome from Clostridium cellulovorans in Bacillus subtilis WB800
2007
Poster presentation - Expression of Cellulosomal Genes from Clostridium cellulovorans in Bacillus subtilis WB800 and Secretion of Their Products
Journals and Meetings Abstract Database at ASM website, 2007
Poster presentation - Effect of Multiple Copies of Cohesins on Cellulase and Hemicellulase Activities of Clostridium cellulovorans Mini-Cellulosomes
Journals and Meetings Abstract Database at ASM website, 2007
Poster presentation
- AMERICAN SOCIETY FOR MICROBIOLOGY
- THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
- JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY
- THE GENETICS SOCIETY OF JAPAN
- Molecular Mechanism of Glyceroglucolipids Involved in Cell Membrane Permeability of Gram-Positive Bacteria
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2022 - 31 Mar. 2025
Saitama University
Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
Grant number：22K05377 - 枯草菌における糖脂質の局在とシャペロン機能の解明
2015 - 2017
Principal investigator
Competitive research funding - A mechanism by which a bacterial outer membrane lipoprotein transmits cell surface stress information to an inner membrane component of a two-component regulatory system
文部科学省, 科学研究費補助金(基盤研究(C)), 2013 - 2016
原 弘志
糖脂質量欠損によるECFシグマの活性化について、予めアンチシグマを欠損しておくと活性化が見られなくなることから、糖脂質（の欠損）が膜タンパク質であるアンチシグマに影響を及ぼしていることが示唆された。また、糖脂質欠損によるECFシグマの活性化はMgイオンの添加で抑制されることが明らかになった。
Competitive research funding - Structure and function of bacterial lipid domains
文部科学省, 科学研究費補助金(基盤研究(C)), 2012 - 2015
松本 幸次
糖脂質欠損によるECFシグマ因子の活性化では、通常の活性化と異なりアンチシグマタンパクの分解を伴わないことをSigV-RsiV系で明らかにした。また異なるストレスに応答するアンチシグマタンパク質のドメイン交換実験から膜貫通領域が糖脂質欠損に対する応答に重要であることが明らかになった。
Competitive research funding - Activation mechanism of a bacterial two-componet system by an outer membrane lipoprotein sensing cell envelope stresses
文部科学省, 科学研究費補助金(基盤研究(C)), Grant-in-Aid for Scientific Research (C), 2010 - 2012
原 弘志, Saitama University
枯草菌野生株を高塩濃度培地に曝すと、糖脂質量が減少・UgtPタンパク量は増加することが明らかとなった。
Competitive research funding, Grant number：22570001 - Structure and formation of bacterial lipid domains
文部科学省, 科学研究費補助金(基盤研究(C)), Grant-in-Aid for Scientific Research (C), 2009 - 2011
松本 幸次, Saitama University
本研究は細菌膜脂質ドメインの構造とその形成機構の解明を目的とし、以下の2つの課題を実施した。(1)膜脂質カルジオリピンとホスファチジルエタノールアミンは枯草菌細胞膜に均一に分布せず、分裂隔壁と両極にドメインをつくり局在する。細胞表層における膜脂質分布の全体像を解明するため、これまで未検討であった他の脂質に対するプローブの開発を進めた。(2)枯草菌細胞の分裂隔壁細胞膜に脂質合成酵素と細胞分裂タンパク質MinDがどの様な仕組みで分裂隔壁の膜に局在するのか、MinD COOH末端の局在に係わる領域を解析し、脂質ドメイン形成の原理解明を進めた。
Competitive research funding, Grant number：21570002 - -
Competitive research funding - -
Competitive research funding