Performance information

• Hen egg only diets support healthy aging in adult mice
  Naomi Nishio; Ken‐ichi Isobe
  Journal of Animal Physiology and Animal Nutrition, Volume：107, Number：4, First page：1110, Last page：1124, Jan. 2023, [Reviewed]
  Abstract
  
  Hen eggs (eggs) are a conventional food, known to contain the nutrients required for the growth of chicken embryos. These eggs are rich in important proteins and fats, with a very low amount of carbohydrate, and include all of the vitamins and minerals needed for the development of mice. We found that mice fed eggs grew to the same weight as mice fed a normal chow diet (ND) and remained healthy until the 20‐months. As expected, the serological indicators of fat content were higher in egg‐only mice than in ND mice. However, surprisingly the serum glucose levels in the egg‐only mice were nearly identical to those in the ND mice. Given the high fat content in eggs, we expected that our egg‐only mice would develop fatty liver or other metabolic diseases. However, we observed no pathological changes in the livers of egg‐only mice until 20‐months with their serological indicators (ALT and AST) and histological features (no fat droplets) remaining normal. However, when we examined the pups nursed by mothers of the egg‐only diet group we noted that almost the animals died 2 to 4 weeks after birth. This is likely because these pups presented with reduced enzymes for metabolism in their liver when compared to pups of the ND group. In addition, we also found that the expression of various development proteins were severely lacking in liver of these pups. From these results, our report suggested that eggs could support healthy aging in adult mice, but not in pups.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/jpn.13805
  DOI ID：10.1111/jpn.13805, ISSN：0931-2439, eISSN：1439-0396
• 鶏卵のみでマウスは生まれ育つか?　　　　　　　　　　　　　　　
  磯部 健一; 西尾 尚美; 加藤 花奈; 鈴木 伶奈; 藤田 遥; 葛谷 亜美; 河村 真凛; 佐藤なつみ
  Volume：13, First page：11, Last page：17, 2021, [Reviewed]
• Hen-egg suppresses fatty liver induced by sugar, glucose or fructose.
  Ken-ichi Isobe; Rena Suzuki; Kana Kato; Miki Kawai; Ami Kuzuya; Aoi Kanjya; Chiaki Suitou; Yui Nakano; Manae Nagasaki; Aki Hatanaka; Asuka Konno; Naomi Nishio
  Journal of Lipid Nutrition, Volume：30, Number：1, First page：43, Last page：59, 2021, [Reviewed]
  Japan Society of Lipid Nutrition, Scientific journal
  DOI：https://doi.org/10.4010/jln.30.43
  DOI ID：10.4010/jln.30.43, ISSN：1343-4594, eISSN：1883-2237
• Are Mice Born Normally With an Egg-only Diet?　　　　　　　　　　　　　　　
  Ken-ichi Isobe; Naomi Nishio
  Volume：3, Number：4, First page：132, Last page：132, Nov. 2020, [Reviewed]
  DOI：https://doi.org/10.31021/brr.20203132
  DOI ID：10.31021/brr.20203132
• Complete genome sequence of emm1 Streptococcus pyogenes 10-85, a strain isolated from a patient with streptococcal toxic shock syndrome in Japan　　　　　　　　　　　　　　　
  Ichiro Tatsuno; Masanori Isaka; Masakado Matsumoto; Naomi Nishio; Hideyuki Matsui; Tadao Hasegawa
  Microbiology Resource Announcements, Volume：8, Number：24, Jan. 2019, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1128/MRA.00453-19
  DOI ID：10.1128/MRA.00453-19, PubMed ID：31196924, PubMed Central ID：PMC6588041, SCOPUS ID：85068464176
• Female GADD34 mice develop age-related inflammation and hepatocellular carcinoma　　　　　　　　　　　　　　　
  Naomi Nishio; Tadao Hasegawa; Ichiro Tatsuno; Masanori Isaka; Ken-ichi Isobe
  GERIATRICS & GERONTOLOGY INTERNATIONAL, Volume：17, Number：12, First page：2593, Last page：2601, Dec. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/ggi.13080
  DOI ID：10.1111/ggi.13080, ISSN：1444-1586, eISSN：1447-0594, PubMed ID：28635009, Web of Science ID：WOS:000418347300043
• Immunological aspects of age-related diseases.　　　　　　　　　　　　　　　
  Ken-Ichi Isobe; Naomi Nishio; Tadao Hasegawa
  World journal of biological chemistry, Volume：8, Number：2, First page：129, Last page：137, May 2017, [Reviewed], ［International magazine］
  The proportion of elderly people rises in the developed countries. The increased susceptibility of the elderly to infectious diseases is caused by immune dysfunction, especially T cell functional decline. Age-related hematopoietic stem cells deviate from lymphoid lineage to myeloid lineage. Thymus shrinks early in life, which is followed by the decline of naïve T cells. T-cell receptor repertoire diversity declines by aging, which is caused by cytomegalovirus-driven T cell clonal expansion. Functional decline of B cell induces antibody affinity declines by aging. Many effector functions including phagocytosis of myeloid cells are down regulated by aging. The studies of aging of myeloid cells have some controversial results. Although M1 macrophages have been shown to be replaced by anti-inflammatory (M2) macrophages by advanced age, many human studies showed that pro-inflammatory cytokines are elevated in older human. To solve this discrepancy here we divide age-related pathological changes into two categories. One is an aging of immune cell itself. Second is involvement of immune cells to age-related pathological changes. Cellular senescence and damaged cells in aged tissue recruit pro-inflammatory M1 macrophages, which produce pro-inflammatory cytokines and proceed to age-related diseases. Underlying biochemical and metabolic studies will open nutritional treatment.
  English, Scientific journal
  DOI：https://doi.org/10.4331/wjbc.v8.i2.129
  DOI ID：10.4331/wjbc.v8.i2.129, PubMed ID：28588756, PubMed Central ID：PMC5439164
• GADD34 Promotes Tumor Growth by Inducing Myeloid-derived Suppressor Cells　　　　　　　　　　　　　　　
  Lintao Liu; Sachiko Ito; Naomi Nishio; Yang Sun; Yuriko Tanaka; Ken-Ichi Isobe
  ANTICANCER RESEARCH, Volume：36, Number：9, First page：4623, Last page：4628, Sep. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.21873/anticanres.11012
  DOI ID：10.21873/anticanres.11012, ISSN：0250-7005, eISSN：1791-7530, PubMed ID：27630304, Web of Science ID：WOS:000384001800032
• Recruitment of Gr1(+)CD11b(+)F4/80(+)Population in the Bone Marrow and Spleen by Irradiation-Induced Pulmonary Damage (vol 38, pg 465, 2015)　　　　　　　　　　　　　　　
  Suganya Thanasegaran; Sachiko Ito; Naomi Nishio; Mohammad Nizam Uddin; Yang Sun; Ken-ichi Isobe
  INFLAMMATION, Volume：39, Number：1, First page：499, Last page：500, Feb. 2016, [Reviewed]
  English
  DOI：https://doi.org/10.1007/s10753-015-0216-z
  DOI ID：10.1007/s10753-015-0216-z, ISSN：0360-3997, eISSN：1573-2576, PubMed ID：26238279, Web of Science ID：WOS:000370083500055
• Thymic Epithelial Cells Induced from Pluripotent Stem Cells by a Three-Dimensional Spheroid Culture System Regenerates Functional T Cells in Nude Mice　　　　　　　　　　　　　　　
  Motohito Okabe; Sachiko Ito; Naomi Nishio; Yuriko Tanaka; Ken-Ichi Isobe
  CELLULAR REPROGRAMMING, Volume：17, Number：5, First page：368, Last page：375, Oct. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1089/cell.2015.0006
  DOI ID：10.1089/cell.2015.0006, ISSN：2152-4971, eISSN：2152-4998, PubMed ID：26348437, Web of Science ID：WOS:000361523600006
• GADD34 Facilitates Cell Death Resulting from Proteasome Inhibition　　　　　　　　　　　　　　　
  Lintao Liu; Sachiko Ito; Naomi Nishio; Yang Sun; Nana Chen; Yuriko Tanaka; Ken-ichi Isobe
  ANTICANCER RESEARCH, Volume：35, Number：10, First page：5317, Last page：5324, Oct. 2015, [Reviewed]
  English, Scientific journal
  ISSN：0250-7005, eISSN：1791-7530, PubMed ID：26408692, Web of Science ID：WOS:000361823200020
• Effects of growth arrest and DNA damage-inducible protein 34 (GADD34) on inflammation-induced colon cancer in mice　　　　　　　　　　　　　　　
  Yuriko Tanaka; Sachiko Ito; Reina Oshino; Nana Chen; Naomi Nishio; Ken-ichi Isobe
  BRITISH JOURNAL OF CANCER, Volume：113, Number：4, First page：669, Last page：679, Aug. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/bjc.2015.263
  DOI ID：10.1038/bjc.2015.263, ISSN：0007-0920, eISSN：1532-1827, PubMed ID：26196182, Web of Science ID：WOS:000359438300013
• GADD34-deficient mice develop obesity, nonalcoholic fatty liver disease, hepatic carcinoma and insulin resistance　　　　　　　　　　　　　　　
  Naomi Nishio; Ken-ichi Isobe
  SCIENTIFIC REPORTS, Volume：5, First page：13519, Aug. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep13519
  DOI ID：10.1038/srep13519, ISSN：2045-2322, PubMed ID：26316333, Web of Science ID：WOS:000360239500001
• Growth arrest and DNA damage-inducible protein (GADD34) enhanced liver inflammation and tumorigenesis in a diethylnitrosamine (DEN)-treated murine model　　　　　　　　　　　　　　　
  Nana Chen; Naomi Nishio; Sachiko Ito; Yuriko Tanaka; Yang Sun; Ken-ichi Isobe
  CANCER IMMUNOLOGY IMMUNOTHERAPY, Volume：64, Number：6, First page：777, Last page：789, Jun. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00262-015-1690-8
  DOI ID：10.1007/s00262-015-1690-8, ISSN：0340-7004, eISSN：1432-0851, PubMed ID：25832002, Web of Science ID：WOS:000355775500014
• Recruitment of Gr1(+)CD11b(+)F4/80(+) Population in the Bone Marrow and Spleen by Irradiation-Induced Pulmonary Damage　　　　　　　　　　　　　　　
  Suganya Thanasegaran; Sachiko Ito; Naomi Nishio; Mohammad Nizam Uddin; Yang Sun; Ken-ichi Isobe
  INFLAMMATION, Volume：38, Number：2, First page：465, Last page：475, Apr. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10753-014-9952-8
  DOI ID：10.1007/s10753-014-9952-8, ISSN：0360-3997, eISSN：1573-2576, PubMed ID：25008148, Web of Science ID：WOS:000350553800001
• GADD34 inhibits activation-induced apoptosis of macrophages through enhancement of autophagy　　　　　　　　　　　　　　　
  Sachiko Ito; Yuriko Tanaka; Reina Oshino; Keiko Aiba; Suganya Thanasegaran; Naomi Nishio; Ken-ichi Isobe
  SCIENTIFIC REPORTS, Volume：5, First page：8327, Feb. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep08327
  DOI ID：10.1038/srep08327, ISSN：2045-2322, PubMed ID：25659802, Web of Science ID：WOS:000348990600016
• Reprint of "iPSCs, aging and age-related diseases"　　　　　　　　　　　　　　　
  Ken-Ichi Isobe; Zhao Cheng; Naomi Nishio; Thanasegan Suganya; Yuriko Tanaka; Sachiko Ito
  NEW BIOTECHNOLOGY, Volume：32, Number：1, First page：169, Last page：179, Jan. 2015, [Reviewed]
  English
  DOI：https://doi.org/10.1016/j.nbt.2014.11.002
  DOI ID：10.1016/j.nbt.2014.11.002, ISSN：1871-6784, eISSN：1876-4347, PubMed ID：25479728, Web of Science ID：WOS:000347507800021
• Enhancement of the Acrolein-Induced Production of Reactive Oxygen Species and Lung Injury by GADD34　　　　　　　　　　　　　　　
  Yang Sun; Sachiko Ito; Naomi Nishio; Yuriko Tanaka; Nana Chen; Lintao Liu; Ken-ichi Isobe
  OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, Volume：2015, First page：170309, 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1155/2015/170309
  DOI ID：10.1155/2015/170309, ISSN：1942-0900, eISSN：1942-0994, PubMed ID：25821552, Web of Science ID：WOS:000351601500001
• iPSCs, aging and age-related diseases　　　　　　　　　　　　　　　
  Ken-ichi Isobe; Zhao Cheng; Naomi Nishio; Thanasegan Suganya; Yuriko Tanaka; Sachiko Ito
  NEW BIOTECHNOLOGY, Volume：31, Number：5, First page：411, Last page：421, Sep. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.nbt.2014.04.004
  DOI ID：10.1016/j.nbt.2014.04.004, ISSN：1871-6784, eISSN：1876-4347, PubMed ID：24784583, Web of Science ID：WOS:000341314700003
• Acrolein induced both pulmonary inflammation and the death of lung epithelial cells　　　　　　　　　　　　　　　
  Yang Sun; Sachiko Ito; Naomi Nishio; Yuriko Tanaka; Nana Chen; Ken-ichi Isobe
  TOXICOLOGY LETTERS, Volume：229, Number：2, First page：384, Last page：392, Sep. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.toxlet.2014.06.021
  DOI ID：10.1016/j.toxlet.2014.06.021, ISSN：0378-4274, eISSN：1879-3169, PubMed ID：24999835, Web of Science ID：WOS:000340045700008
• Negative Regulation of GADD34 on Myofibroblasts during Cutaneous Wound Healing　　　　　　　　　　　　　　　
  Lintao Liu; Naomi Nishio; Sachiko Ito; Yuriko Tanaka; Ken-ichi Isobe
  BIOMED RESEARCH INTERNATIONAL, Volume：2014, First page：137049, 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1155/2014/137049
  DOI ID：10.1155/2014/137049, ISSN：2314-6133, eISSN：2314-6141, PubMed ID：25210702, Web of Science ID：WOS:000347824500001
• [Accuracy estimation of non-invasive X-ray output analyzer].　　　　　　　　　　　　　　　
  Ioka Y; Ariga E; Nishio N; Ooshima T
  Nihon Hoshasen Gijutsu Gakkai zasshi, Volume：69, Number：10, First page：1153, Last page：1160, Oct. 2013, [Reviewed]
  This study estimated the accuracy of an X-ray analyzer by comparing it with an ionization chamber and a tube voltage current meter, and investigated whether it was usable as a substitute for a reference meter for output measurements for quality control purposes. The X-ray output analyzer used was a Piranha (RTI Electronics), a non-invasive instrument. The two subjects of measurements were as follows: the tube voltage, exposure, and half-value layer used in ordinary X-ray radiographic system equipment, and the exposure and half-value layer in X-ray equipment for mammographic systems. The results for a conventional radiographic system showed the error rates for tube voltage, exposure, and half value layer to be within ±1.0%, ±1.8%, and ±4.3%, respectively. The Piranha is not influenced by the dependence of the beam quality in a range of the tube voltage in clinical use. In X-ray equipment for mammographic system results, error rates for exposure and half value layer were ±2.2% and within ±4.0%, respectively. We conclude that it is possible to use the Piranha as an alternative reference meter for quality control of X-ray equipment for typical radiographic and mammographic systems.
  Japanese Society of Radiological Technology, Japanese
  DOI：https://doi.org/10.6009/jjrt.2013_JSRT_69.10.1153
  DOI ID：10.6009/jjrt.2013_JSRT_69.10.1153, ISSN：0369-4305, CiNii Articles ID：10031196868, CiNii Books ID：AN00197784, PubMed ID：24140904
• Characteristics of cardiac aging in C57BL/6 mice　　　　　　　　　　　　　　　
  Zhao Cheng; Sachiko Ito; Naomi Nishio; Suganya Thanasegaran; He Fang; Ken-ichi Isobe
  EXPERIMENTAL GERONTOLOGY, Volume：48, Number：3, First page：341, Last page：348, Mar. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.exger.2013.01.005
  DOI ID：10.1016/j.exger.2013.01.005, ISSN：0531-5565, PubMed ID：23337778, Web of Science ID：WOS:000315683800006
• iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis　　　　　　　　　　　　　　　
  Tetsutaro Kito; Rei Shibata; Masakazu Ishii; Hirohiko Suzuki; Tatsuhito Himeno; Yoshiyuki Kataoka; Yumiko Yamamura; Takashi Yamamoto; Naomi Nishio; Sachiko Ito; Yasushi Numaguchi; Tohru Tanigawa; Jun K. Yamashita; Noriyuki Ouchi; Hiroyuki Honda; Kenichi Isobe; Toyoaki Murohara
  SCIENTIFIC REPORTS, Volume：3, First page：1418, Mar. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep01418
  DOI ID：10.1038/srep01418, ISSN：2045-2322, PubMed ID：23475393, Web of Science ID：WOS:000315852100004
• No immunogenicity of IPS cells in syngeneic host studied by in vivo injection and 3D scaffold experiments.　　　　　　　　　　　　　　　
  Suganya Thanasegaran; Zhao Cheng; Sachiko Ito; Naomi Nishio; Ken-ichi Isobe
  BioMed research international, Volume：2013, First page：378207, Last page：378207, 2013, [Reviewed], ［International magazine］
  Induced Pluripotent Stem Cells (IPSCs) open the great possibility to employ patient's own tissue to the previously incurable diseases. However these cells can be used in cell therapy only if they are not rejected when transplanted back into the syngeneic host. We found that the injection of iPSCs derived from different ages of mice into syngeneic C57BL/6 mice produced teratoma and was not rejected. Then we cultured iPSCs and myeloid differentiated iPSCs in three-dimensional porous scaffold and transplanted to C57BL/6 mice and BALB/C mice. After transplantation, we could observe the cell density inside the scaffold increased rapidly in syngeneic mice compared to the allogeneic mice indicating the favorable conditions supporting the growth of iPSCs in vivo. Unlike the allogeneic counterpart, we could not observe few infiltrating T cells inside the scaffold of syngeneic mice. These results contribute to the optimistic view of iPSCs for regenerative medicine in near future.
  English, Scientific journal
  DOI：https://doi.org/10.1155/2013/378207
  DOI ID：10.1155/2013/378207, ISSN：2314-6133, PubMed ID：23691499, PubMed Central ID：PMC3652117
• Establishment of self-renewable GM-CSF-dependent immature macrophages in vitro from murine bone marrow.　　　　　　　　　　　　　　　
  Sachiko Ito; Yuriko Tanaka; Naomi Nishio; Suganya Thanasegaran; Ken-Ichi Isobe
  PloS one, Volume：8, Number：10, First page：e76943, 2013, [Reviewed], ［International magazine］
  Macrophages play a key role in the innate immune system. Macrophages are thought to originate from hematopoietic precursors or the yolk sac. Here, we describe the in vitro establishment of self-renewable GM-CSF-dependent immature macrophages (GM-IMs) from murine bone marrow (BM). GM-IMs grow continuously in vitro in conditioned medium containing GM-CSF. The immunophenotype of GM-IMs is F4/80(high) CD11b(high) CD11c(low) Ly6C(low). By comparing gene expression in GM-IMs and BM dendritic cells, we found that GM-IMs expressed lower levels of chemokines, cytokines and their receptors. GM-IMs are round in shape, attach loosely to non-coated culture dishes and have a marked phagocytic capacity. These results indicate that GM-IMs are macrophage precursor cells. Following stimulation with LPS, monocyte-like GM-IMs converted to flat macrophage-like cells that tightly adhered to non-coated culture dishes and produced pro-inflammatory cytokines TNFα, IL-6 and IL-1β. These results indicated that GM-IMs differentiated to M1 pro-inflammatory macrophages. This was confirmed by stimulation of GM-IMs with IFNγ, an inducer of M1 markers. GM-IMs showed enhanced expression of M2 macrophage markers such as Arg1 and Retnla following stimulation by Th2 cytokines IL-4 and IL-13. When GM-IMs were injected into mice at sites of wounding, wound repair was enhanced. These results indicate that GM-IMs can differentiate to M2 macrophages. When GM-IMs were injected into clodronate-treated mice, they induced resident macrophage proliferation by producing M-CSF. In conclusion we have established self-renewable GM-CSF-dependent immature macrophages in vitro from murine BM, which differentiate to M1 or M2 macrophages.
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0076943
  DOI ID：10.1371/journal.pone.0076943, PubMed ID：24124601, PubMed Central ID：PMC3790761
• Comparative Angiogenic Activities of Induced Pluripotent Stem Cells Derived from Young and Old Mice　　　　　　　　　　　　　　　
  Hirohiko Suzuki; Rei Shibata; Tetsutaro Kito; Takashi Yamamoto; Masakazu Ishii; Naomi Nishio; Sachiko Ito; Ken-ichi Isobe; Toyoaki Murohara
  PLOS ONE, Volume：7, Number：6, First page：e39562, Jun. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0039562
  DOI ID：10.1371/journal.pone.0039562, ISSN：1932-6203, PubMed ID：22761825, Web of Science ID：WOS:000305825800043
• Autophagic activity in thymus and liver during aging　　　　　　　　　　　　　　　
  Mohammad Nizam Uddin; Naomi Nishio; Sachiko Ito; Haruhiko Suzuki; Ken-ichi Isobe
  AGE, Volume：34, Number：1, First page：75, Last page：85, Feb. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11357-011-9221-9
  DOI ID：10.1007/s11357-011-9221-9, ISSN：0161-9152, PubMed ID：21387084, Web of Science ID：WOS:000299294100007
• Analysis of β-Amyloid Peptide -Binding Proteins in Microglial Cells　　　　　　　　　　　　　　　
  Sachiko Ito
  The Open Geriatric Medicine Journal, Volume：5, Number：1, First page：1, Last page：6, Jan. 2012, [Reviewed]
  Bentham Science Publishers Ltd., Scientific journal
  DOI：https://doi.org/10.2174/1874827901205010001
  DOI ID：10.2174/1874827901205010001, ISSN：1874-8279
• 3D-aggregated dermal stem cells with partial-pluripotency　　　　　　　　　　　　　　　
  Masaki Kondo; Hideki Kamiya; Tetsuji Okawa; Sachiko Ito; Naomi Nishio; Tatsuhito Himeno; Yutaka Oiso; Jiro Nakamura; Ken-Ichi Isobe
  2012 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2012, First page：156, Last page：159, 2012, [Reviewed]
  English, International conference proceedings
  DOI：https://doi.org/10.1109/MHS.2012.6492401
  DOI ID：10.1109/MHS.2012.6492401, SCOPUS ID：84876543437
• Establishment of neutrophil-lineage stem cells from C57BL/6 mice.　　　　　　　　　　　　　　　
  Naomi Nishio; Sachiko Ito; Yuriko Tanaka; Ken-ichi Isobe
  2012 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), First page：160, Last page：163, 2012, [Reviewed]
  English, International conference proceedings
  ISSN：2474-378X, eISSN：2474-3798, Web of Science ID：WOS:000318860500034
• Possibility to use iPS-technology in age-related diseases　　　　　　　　　　　　　　　
  Zhao Cheng; Sachiko Ito; Naomi Nishio; Thanasegaran Suganya; Ken-ichi Isobe
  2012 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), First page：116, Last page：120, 2012, [Reviewed]
  English, International conference proceedings
  ISSN：2474-378X, eISSN：2474-3798, Web of Science ID：WOS:000318860500026
• Aging in the mouse and perspectives of rejuvenation through induced pluripotent stem cells (iPSCs).　　　　　　　　　　　　　　　
  Ken-ichi Isobe; Zhao Cheng; Sachiko Ito; Naomi Nishio
  Results and problems in cell differentiation, Volume：55, First page：413, Last page：27, 2012, [Reviewed], ［International magazine］
  The mouse is a perfect model to study aging in mammals. It has a relatively short life span and genetic manipulations in this species are well established. Most interestingly, the mouse is a fantastic tool to produce stem cells. Forced expression of only four transcription factors (Oct3/4, Sox2, Klf4, and c-Myc) in murine and human somatic cells resets the expression of genes that are characteristic of differentiated cells and consequently induces the formation of pluripotent stem cells (iPSCs). This technology opens new and exciting possibilities in medical research, especially personalized cell therapies for treating human disease. To treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPSCs from their tissues. To determine the feasibility of using this technology with elderly patients, we asked whether it was indeed possible to establish iPSCs from the tissues of aged mice and to differentiate them to tissue cells. We succeeded in establishing iPSC clones using bone marrow (BM) from 21-month-old EGFP-C57BL/6 mice, which had been cultured for 4 days in the presence of granulocyte macrophage-colony stimulating factor (GM-CSF). Our iPSCs from aged mice (aged iPSCs) and those from mouse embryonic fibroblasts (MEFs) strongly expressed SSEA-1 and Pou5f1, and showed strong alkaline phosphatase (AP) activity. Our aged iPSCs made teratomas when injected into the back skin of syngeneic mice, and differentiated to tissue cells of three germ lines in vitro. Further experiments to make chimeric mice and germ line cells will determine whether the aged iPSCs possess the properties of much younger cells and are capable of regenerating aged mice.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-3-642-30406-4_21
  DOI ID：10.1007/978-3-642-30406-4_21, ISSN：0080-1844, PubMed ID：22918818
• Dextran sulphate sodium increases splenic Gr1+CD11b+ cells which accelerate recovery from colitis following intravenous transplantation　　　　　　　　　　　　　　　
  R. Zhang; S. Ito; N. Nishio; Z. Cheng; H. Suzuki; K. I. Isobe
  Clinical and Experimental Immunology, Volume：164, Number：3, First page：417, Last page：427, Jun. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-2249.2011.04374.x
  DOI ID：10.1111/j.1365-2249.2011.04374.x, ISSN：0009-9104, PubMed ID：21413942, SCOPUS ID：79955417245
• Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells　　　　　　　　　　　　　　　
  Zhao Cheng; Sachiko Ito; Naomi Nishio; Hengyi Xiao; Rong Zhang; Haruhiko Suzuki; Yayoi Okawa; Toyoaki Murohara; Ken-ichi Isobe
  JOURNAL OF MOLECULAR CELL BIOLOGY, Volume：3, Number：2, First page：91, Last page：98, Apr. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jmcb/mjq044
  DOI ID：10.1093/jmcb/mjq044, ISSN：1674-2788, eISSN：1759-4685, PubMed ID：21228011, Web of Science ID：WOS:000289306800004
• Gadd34 induces autophagy through the suppression of the mTOR pathway during starvation　　　　　　　　　　　　　　　
  Mohammad Nizam Uddin; Sachiko Ito; Naomi Nishio; Thanasegaran Suganya; Ken-ichi Isobe
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume：407, Number：4, First page：692, Last page：698, Apr. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.bbrc.2011.03.077
  DOI ID：10.1016/j.bbrc.2011.03.077, ISSN：0006-291X, PubMed ID：21439266, Web of Science ID：WOS:000290240500012
• Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype　　　　　　　　　　　　　　　
  Yuta Inami; Tohru Yoshikai; Sachiko Ito; Naomi Nishio; Haruhiko Suzuki; Hidetoshi Sakurai; Ken-Ichi Isobe
  IMMUNOLOGY AND CELL BIOLOGY, Volume：89, Number：2, First page：314, Last page：321, Feb. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/icb.2010.96
  DOI ID：10.1038/icb.2010.96, ISSN：0818-9641, PubMed ID：20680027, Web of Science ID：WOS:000287445400021
• Up-regulation of Gr1+CD11b+ population in spleen of dextran sulfate sodium administered mice works to repair colitis.　　　　　　　　　　　　　　　
  Rong Zhang; Sachiko Ito; Naomi Nishio; Zhao Cheng; Haruhiko Suzuki; Ken-ichi Isobe
  Inflammation & allergy drug targets, Volume：10, Number：1, First page：39, Last page：46, Feb. 2011, [Reviewed], ［International magazine］
  Dextran sulfate sodium (DSS) is commonly used in rodent IBD models to chemically induce acute intestinal inflammation. The acute course of colitis includes colon tissue damages and recovery from wounded tissues. As skin wound repair was delayed by splenectomy, we asked whether splenectomy would induce the delay of colonic wound healing. In splenectomized mice, body weight recovery, disease score and colon length were delayed. Surprisingly we found a great increase of Gr1+CD11b+ cells in spleen and bone marrow of DSS-administered mice. Anti-Gr-1 antibody treatment worsened the DSS- administered colitis. These results indicate that Gr1+CD11b+ cells induced by DSS worked to repair colon wound healing and repair colitis.
  English, Scientific journal
  ISSN：1871-5281, eISSN：2212-4055, PubMed ID：21184649
• Toxic effects of D-galactose on thymus and spleen that resemble aging　　　　　　　　　　　　　　　
  Mohammad Nizam Uddin; Naomi Nishio; Sachiko Ito; Haruhiko Suzuki; Ken-ichi Isobe
  JOURNAL OF IMMUNOTOXICOLOGY, Volume：7, Number：3, First page：165, Last page：173, Sep. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.3109/15476910903510806
  DOI ID：10.3109/15476910903510806, ISSN：1547-691X, PubMed ID：20050818, Web of Science ID：WOS:000281919600003
• Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells　　　　　　　　　　　　　　　
  Hirohiko Suzuki; Rei Shibata; Tetsutaro Kito; Masakazu Ishii; Ping Li; Toru Yoshikai; Naomi Nishio; Sachiko Ito; Yasushi Numaguchi; Jun K. Yamashita; Toyoaki Murohara; Kenichi Isobe
  BMC CELL BIOLOGY, Volume：11, First page：72, Sep. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1186/1471-2121-11-72
  DOI ID：10.1186/1471-2121-11-72, ISSN：1471-2121, PubMed ID：20860813, Web of Science ID：WOS:000283075600001
• GADD34 suppresses wound healing by upregulating expression of myosin IIA　　　　　　　　　　　　　　　
  Chie Tanaka; Sachiko Ito; Naomi Nishio; Yasuhiro Kodera; Hidetoshi Sakurai; Haruhiko Suzuki; Akimasa Nakao; Ken-Ichi Isobe
  TRANSGENIC RESEARCH, Volume：19, Number：4, First page：637, Last page：645, Aug. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11248-009-9347-z
  DOI ID：10.1007/s11248-009-9347-z, ISSN：0962-8819, PubMed ID：20625881, Web of Science ID：WOS:000279844300011
• Effect of Aging on Auto-Antibodies to Wounded Tissues~!2010-03-17~!2010-04-30~!2010-06-17~!　　　　　　　　　　　　　　　
  Naomi Nishio; Sachiko Ito; Haruhiko Suzuki; Yayoi Okawa; Ken-ichi Isobe
  The Open Geriatric Medicine Journal, Volume：3, Number：1, First page：17, Last page：24, Jul. 2010, [Reviewed]
  Bentham Science Publishers Ltd., Scientific journal
  DOI：https://doi.org/10.2174/1874827901003010017
  DOI ID：10.2174/1874827901003010017, ISSN：1874-8279
• The wound repair is control by monocyte linage cells　　　　　　　　　　　　　　　
  Naomi Nishio; Sachiko Ito; Yayoi Okawa; Ken-Ichi Isobe
  2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010, First page：278, Last page：282, 2010, [Reviewed]
  English, International conference proceedings
  DOI：https://doi.org/10.1109/MHS.2010.5669544
  DOI ID：10.1109/MHS.2010.5669544, SCOPUS ID：78751481881
• Antibodies to wounded tissue enhance cutaneous wound healing　　　　　　　　　　　　　　　
  Naomi Nishio; Sachiko Ito; Haruhiko Suzuki; Ken-Ichi Isobe
  IMMUNOLOGY, Volume：128, Number：3, First page：369, Last page：380, Nov. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-2567.2009.03119.x
  DOI ID：10.1111/j.1365-2567.2009.03119.x, ISSN：0019-2805, PubMed ID：20067537, Web of Science ID：WOS:000270588300007
• [Age-related decline of immune function and age-related diseases].　　　　　　　　　　　　　　　
  Ken-ichi Isobe; Naomi Nishio; Sachiko Ito
  Nihon rinsho. Japanese journal of clinical medicine, Volume：67, Number：7, First page：1327, Last page：31, Jul. 2009, [Reviewed], ［Domestic magazine］
  Effects of aging on immune system are widespread. The development of T and B cells declines with age. The functions of matured T and B cell also decline with age. Consequently, infections present major clinical problems for elderly patients. Many of age-related diseases are related to innate immunity. For example, the pathogenesis of atherosclerosis and Alzheimer disease are related to macrophages (microglia). The Ox-LDL or A-beta induces macrophages or microglia to produce inflammatory cytokines, chemokines and matrix metalloproteinases. Recently neutrophils have been shown to be an important immune cells in atherosclerosis. Neutrophils secrete inflammatory cytokines. In wound healing neutrophils also work as first important immune cells, which affect age-related decline of wound repair.
  Japanese, Scientific journal
  ISSN：0047-1852, PubMed ID：19591280
• Injection of bleomycin in newborn mice induces autoimmune sialitis that is transferred by CD4 T cells　　　　　　　　　　　　　　　
  Hideaki Ishikawa; Sachiko Ito; Naomi Nishio; Yukio Yuzawa; Sei-ichi Matsuo; Ken-ichi Isobe
  IMMUNOLOGY AND CELL BIOLOGY, Volume：87, Number：4, First page：351, Last page：358, May 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/icb.2009.1
  DOI ID：10.1038/icb.2009.1, ISSN：0818-9641, PubMed ID：19204734, Web of Science ID：WOS:000266208800013
• Thymic Involution Correlates with Severe Ulcerative Colitis Induced by Oral Administration of Dextran Sulphate Sodium in C57BL/6 Mice but not in BALB/c Mice　　　　　　　　　　　　　　　
  Shin Sasaki; Yoshiyuki Ishida; Naomi Nishio; Sachiko Ito; Ken-ichi Isobe
  INFLAMMATION, Volume：31, Number：5, First page：319, Last page：328, Oct. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10753-008-9081-3
  DOI ID：10.1007/s10753-008-9081-3, ISSN：0360-3997, PubMed ID：18696222, Web of Science ID：WOS:000260059800005
• Neutrophil depletion delays wound repair in aged mice　　　　　　　　　　　　　　　
  Naomi Nishio; Yayoi Okawa; Hidetoshi Sakurai; Ken-ichi Isobe
  AGE, Volume：30, Number：1, First page：11, Last page：19, Mar. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11357-007-9043-y
  DOI ID：10.1007/s11357-007-9043-y, ISSN：0161-9152, PubMed ID：19424869, Web of Science ID：WOS:000255964700002
• Efficient induction of osteogenic and chondrogenic progenitors and myogenic progenitors from mouse ES cells in chemically defined medium　　　　　　　　　　　　　　　
  Hidetoshi Sakurai; Yuta Inami; Naomi Nishio; Tohru Yoshikai; Ken-Ichi Isobe
  2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, First page：487, Last page：490, 2008, [Reviewed]
  English, International conference proceedings
  DOI：https://doi.org/10.1109/MHS.2008.4752501
  DOI ID：10.1109/MHS.2008.4752501, SCOPUS ID：62449169784
• Paraxial mesodermal progenitors derived from mouse embryonic stem cells contribute to muscle regeneration via differentiation into muscle satellite cells　　　　　　　　　　　　　　　
  Hidetoshi Sakurai; Yayoi Okawa; Yuta Inami; Naomi Nishio; Ken-ichi Isobea
  STEM CELLS, Volume：26, Number：7, First page：1865, Last page：1873, 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1634/stemcells.2008-0173
  DOI ID：10.1634/stemcells.2008-0173, ISSN：1066-5099, PubMed ID：18450822, Web of Science ID：WOS:000258004400022
• Differential effects of physical and psychological stressors on immune functions of rats　　　　　　　　　　　　　　　
  K Oishi; N Nishio; K Konishi; M Shimokawa; T Okuda; T Kuriyama; K Machida
  STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, Volume：6, Number：1, First page：33, Last page：40, Mar. 2003, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1080/1025389031000101330
  DOI ID：10.1080/1025389031000101330, ISSN：1025-3890, PubMed ID：12637205, Web of Science ID：WOS:000184580400006
• SST-2 tumor inoculation is a useful model for studying the anti-tumor immune response in SHR rats　　　　　　　　　　　　　　　
  Naomi Nishio; Katsutaka Oishi; Kazuhiko Machida
  Environmental Health and Preventive Medicine, Volume：8, Number：1, First page：1, Last page：5, 2003, [Reviewed]
  Japanese Society for Hygiene, English, Scientific journal
  DOI：https://doi.org/10.1007/BF02897936
  DOI ID：10.1007/BF02897936, ISSN：1342-078X, PubMed ID：21432108, SCOPUS ID：0037358027
• [The effect of chronic stress on neutrophil function in tumor-inoculated rats].　　　　　　　　　　　　　　　
  Nishio N; Fujioka Y; Midorikawa T; Ishizaki K; Machida K
  Nihon eiseigaku zasshi. Japanese journal of hygiene, Volume：57, Number：4, First page：655, Last page：660, Jan. 2003, [Reviewed]
  Objectives: In order to clarify the relation between stress load and inoculated tumor growth in conjunction with neutrophil functions, several kinds of stress such as physical (Ph) and psychological (Ps) stress were loaded on rats either SST-2 tumor cell inoculated or control, and the functions of their peripheral neutrophils were determined.
  Methods: A communication box was used for stress load on rats. SST-2 cells were inoculated in to rats in the tumor-inoculation groups. Two weeks after inoculation, the tumors were removed from the backs of the rats and weighed. The functions of neutrophils in the peripheral blood collected from the tail vein, were determined by the NBT deoxidization method.
  Results: Tumor growth was enhanced when rats were loaded with either Ph or Ps stresses, but was inhibited when tumor cells were inoculated following either Ph or Ps stresses. These results show that chronological differences of loaded stresses influence immunological functions differently. The O₂^- production from the neutrophils stimulated by NBT-treated Staphilococcus aureus was suppressed in tumor-inoculated Ph and Ps groups, more markedly in the tumor-inoculated Ph group. It is logically relevant that the size of tumors increased in these groups, predominantly in the tumor-inoculated Ph group. On the other hand, O₂^- production from the neutrophils was enhanced and tumor growth decreased in tumor-inoculated animals following either Ph or Ps stresses.
  Conclusions: Our experiments, it revealed that the function of neutrophils is strongly enhanced by stress load and O₂^- production is inhibited by the tumor inoculation as shown in stimulation tests. Therefore, our findings suggested that neutrophils may participate in the inhibition of tumor growth.
  The Japanese Society for Hygiene, Japanese
  DOI：https://doi.org/10.1265/jjh.57.655
  DOI ID：10.1265/jjh.57.655, ISSN：0021-5082, CiNii Articles ID：130000997718, CiNii Books ID：AN00185923, PubMed ID：12638170

• Aging and immune system　　　　　　　　　　　　　　　
  ISOBE Ken-ichi; ITO Sachiko; NISHIO Naomi
  Japanese journal of geriatrics, Volume：48, Number：3, First page：205, Last page：210, 25 May 2011
  Japanese
  ISSN：0300-9173, CiNii Articles ID：10030469561, CiNii Books ID：AN00199010
• The effects of physical and pshychological stress on rat injected tumor cells : (2) Nonspecific defense function　　　　　　　　　　　　　　　
  NISHIO Naomi
  Volume：54, Number：1, First page：384, Last page：384, 15 Apr. 1999
  Japanese
  ISSN：0021-5082, CiNii Articles ID：10013389224, CiNii Books ID：AN00185923
• The effects of physical and pshychological stress on rat injected tumor cells : (1) Biological function　　　　　　　　　　　　　　　
  FUJIOKA Yosei
  Volume：54, Number：1, First page：383, Last page：383, 15 Apr. 1999
  Japanese
  ISSN：0021-5082, CiNii Articles ID：10013389223, CiNii Books ID：AN00185923
• Relationship between amount of physical activity and health in the elderly　　　　　　　　　　　　　　　
  SASAKI Kaiho
  Volume：54, Number：1, First page：278, Last page：278, 15 Apr. 1999
  Japanese
  ISSN：0021-5082, CiNii Articles ID：10013389102, CiNii Books ID：AN00185923
• Effects of host defense function in student Triathlete : (1) Change of pre and post in triathlon race　　　　　　　　　　　　　　　
  ISHIZAKI Kaori
  Volume：54, Number：1, First page：276, Last page：276, 15 Apr. 1999
  Japanese
  ISSN：0021-5082, CiNii Articles ID：10013389100, CiNii Books ID：AN00185923
• Effects of exercise practice on biochemistry test in elderly　　　　　　　　　　　　　　　
  KITAYAMA Seita
  Volume：54, Number：1, First page：279, Last page：279, 15 Apr. 1999
  Japanese
  ISSN：0021-5082, CiNii Articles ID：10013389103, CiNii Books ID：AN00185923

• Apr. 2018 - Present
  微生物学(免疫学を含む)
• Apr. 2018 - Present
  生理学
• Apr. 2018 - Present
  解剖生理学
• Apr. 2018 - Present
  臨床実習
• 2018 - Present
  栄養学(食品学を含む)
• Apr. 2019 - Mar. 2022
  薬理概論
• Oct. 2018 - Mar. 2022
  physiology, Tokyo Gakugei University
• Oct. 2019 - Mar. 2020
  栄養学
• Apr. 2018
  解剖生理学実習

• Apr. 2025 - Present
• Jun. 2007 - Present
• Apr. 1997 - Present, THE JAPANESE SOCIETY FOR HYGIENE

• 「チーム学校」による心身緊急時等対応体制構築のための教育プログラム開発と情報支援　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2027
  Grant amount(Total)：16510000, Direct funding：12700000, Indirect funding：3810000
  Grant number：22H00961
• GADD34による老化関連疾患の制御メカニズムの解析　　　　　　　　　　　　　　　
  01 Apr. 2019 - 31 Mar. 2023
  Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
  Grant number：19K11687
• Stress responsive GADD34 and metabolic syndrome　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2016 - 31 Mar. 2019
  isobe ken-ichi, Nagoya Women's University
  Grant amount(Total)：17290000, Direct funding：13300000, Indirect funding：3990000
  metabolic syndrome represent serious in Japan. We found that aged GADD34 -deficient mice showed fatty liver.We examined the effects of GADD34 on natural life span by using GADD34-deficient mice. We observed that with age GADD34-deficient mice become obese, developing fatty liver followed by liver cirrhosis, hepatocellular carcinoma and insulin resistance. During these studies, we found that nutrients in the foods were important to induce fatty liver. Feeding of sugar, glucose or fructose only diet induced strong nonalcoholic fatty liver disease only in 2 weeks.. High fats (60% fat) without choline diet also induced fatty liver in 2 weeks. For more than one year feeding of adult C57BL/6 female mice egg-only diet did not induce fatty liver. Adding boiled eggs or law egg yolk to sugar, glucose or fructose or only diet or colline deficient diet strongly suppressed fatty liver.
  Grant number：16H05280
• Age-related diseases analyzed by ER stresses and immune functions using murine models including iPSCs　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2013 - 31 Mar. 2017
  isobe ken-ichi
  Grant amount(Total)：18330000, Direct funding：14100000, Indirect funding：4230000
  We intended to analyze age-related disease from the points of ER stresses and immune responses. Acrolein, which is abundant in cigarette smoke, and cooking emission,
  plays a major role in COPD . Acrolein induced the expression of GADD34 and acute inflammation in airways, which followed COPD. Acrolein-induced phosphorylation of eIF2α in GADD34-knockout epithelial cells by shRNA protected cell death by reducing misfolded protein-caused oxidative stress. We examine the effects of GADD34 on natural life span by using GADD34-deficient mice. We found that with age GADD34-deficient mice become obese, developing fatty liver followed by liver cirrhosis, hepatocellular carcinoma, and insulin resistance. GADD34 suppresses insulin signaling in young mice. However, by aging macrophages in fat tissues work to induce insulin resistance. We succeeded to differentiate to various tissue cells from iPSCs established from aged mice, which may repair damaged tissues by age-related diseases.
  Grant number：25293166