Performance information

• Impact of Nigrospora oryzae-Derived Natural Products on Photosynthesis and Oxidative Stress in Eichhornia crassipes.　　　　　　　　　　　　　　　
  Chamroon Laosinwattana; Nutcha Manichart; Pattharin Wichittrakarn; Kaori Yoneyama; Montinee Teerarak; Hataichanok Passara
  Physiologia plantarum, Volume：177, Number：1, First page：e70104, Feb. 2025, [Reviewed], ［International magazine］
  Interest in natural herbicides has been growing due to government policies restricting synthetic herbicide use in many countries. In that regard, this study investigates the potential of Nigrospora oryzae extract as a natural herbicide against the aquatic invasive weed Eichhornia crassipes. A stable formulation was developed with a droplet size of 36.44 ± 0.36 nm and a zeta potential of -62.59 mV. Pot-based experiments revealed the N. oryzae extract induced 38.33% phytotoxicity within 24 hours, increasing to 84.72% by 28 days post-treatment. Scanning electron microscopy demonstrated morphoanatomical changes in epidermal tissue and stroma of E. crassipes, such as erosion of epicuticular waxes and degeneration of epidermis cells. The treatment significantly reduced the photosynthetic pigment content while increasing hydrogen peroxide (46.26%), malondialdehyde (17.49%), and proline (19.16%) levels, causing cellular electrolyte leakage. Activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase were significantly elevated (p<0.05), indicating oxidative damage. These findings demonstrate that N. oryzae extract can disrupt growth and photosynthesis and induce oxidative stress in E. crassipes, suggesting its potential as a source of natural herbicide for industrial application.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.70104
  DOI ID：10.1111/ppl.70104, PubMed ID：39901629, PubMed Central ID：PMC11791469
• Evolution of interorganismal strigolactone biosynthesis in seed plants　　　　　　　　　　　　　　　
  Anqi Zhou; Annalise Kane; Sheng Wu; Kaibiao Wang; Michell Santiago; Yui Ishiguro; Kaori Yoneyama; Malathy Palayam; Nitzan Shabek; Xiaonan Xie; David C. Nelson; Yanran Li
  Science, Volume：387, Number：6731, First page：eadp0779, Jan. 2025, [Reviewed], ［International magazine］
  Strigolactones (SLs) are methylbutenolide molecules derived from β-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A , an evolutionary predecessor of CYP722C . CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.
  American Association for the Advancement of Science (AAAS), English, Scientific journal
  DOI：https://doi.org/10.1126/science.adp0779
  DOI ID：10.1126/science.adp0779, ISSN：0036-8075, eISSN：1095-9203, PubMed ID：39818909
• Nitrogen deficiency influences strigolactone levels in basal parts of shoots and shoot branching phenotype in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  Riko Sakioka; Kaori Yoneyama
  Bioscience, biotechnology, and biochemistry, Dec. 2024, [Reviewed], [Corresponding], ［International magazine］
  Strigolactones (SLs) are allelochemicals attracting both symbiotic arbuscular mycorrhizal (AM) fungi and root parasitic plants and a novel class of phytohormones inhibiting lateral shoot branching. In general, nutrient deficiency significantly promotes SL production in roots and exudation into the rhizosphere, and also induces suppressed shoot branching phenotype in host plants of AM fungi including rice, tomato, maize, etc. Nutrient deficiencies also inhibit the shoot branching in Arabidopsis thaliana (hereafter Arabidopsis), a non-host of AM fungi, while the level of carlactone, a non-canonical SL and the SL precursor for the other SLs, was reported to be unaffected. Because Arabidopsis SLs including CL and methyl carlactonoate,, are highly unstable, relationships between SL levels and shoot branching in Arabidopsis remain elusive. Herein, we demonstrate that nitrogen deficiency increases SL levels in the basal part of shoots in Arabidopsis and lateral shoot branching appears to be strongly regulated by these SLs.
  English, Scientific journal
  DOI：https://doi.org/10.1093/bbb/zbae185
  DOI ID：10.1093/bbb/zbae185, PubMed ID：39657996
• Oxidative damage in Echinochloa crus-galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination　　　　　　　　　　　　　　　
  Naphat Somala; Nutcha Manichart; Chamroon Laosinwattana; Pattharin Wichittrakarn; Kaori Yoneyama; Montinee Teerarak; Nawasit Chotsaeng
  FRONTIERS IN AGRONOMY, Volume：6, Nov. 2024, [Reviewed]
  This study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl acetate (EtOAc) fraction most greatly reduced seed germination (81.01%), root length (89.18%), and shoot length (84.74%) compared to the control. Chemical characterization using gas chromatography-mass spectrometry revealed major constituents of linoleic acid, butyl ester (9.69%), hexadecanoic acid (7.99%), and 14-pentadecenoic acid (7.86%). With regard to physiological and biochemical indexes, treated seeds exhibited lower imbibition, significantly decreased alpha-amylase (EC 3.2.1.1) activity (p<0.05), and increased accumulation of malondialdehyde (85.52%) and hydrogen peroxide (141.10%). Moreover, activity of the antioxidant enzymes superoxide dismutase (EC 1.15.1.1) and guaiacol peroxidase (EC 1.11.1.7) was upregulated (67.24 and 61.62%, respectively), while catalase (EC 1.11.1.6) activity was downregulated (-33.75%). The inference is that an imbalance in ROS levels combined with reduced antioxidant potential drives the gradual accumulation of oxidative damage in seed cells and consequent loss of seed viability. All told, these results confirm the Diaporthe extract to induce oxidative stress and inhibit antioxidant enzymes. This study clearly demonstrates the oxidative damage associated with Diaporthe allelochemicals.
  FRONTIERS MEDIA SA, English, Scientific journal
  DOI：https://doi.org/10.3389/fagro.2024.1456168
  DOI ID：10.3389/fagro.2024.1456168, eISSN：2673-3218, Web of Science ID：WOS:001373236600001
• Barley is a potential trap crop for root parasitic broomrape weeds.　　　　　　　　　　　　　　　
  Maiko Inoue; Xiaonan Xie; Kaori Yoneyama
  Journal of pesticide science, Volume：49, Number：4, First page：255, Last page：261, Nov. 2024, [Reviewed], [Corresponding], ［Domestic magazine］
  Root parasitic broomrape (Phelipanche and Orobanche spp.) weeds cause devastating damage to agricultural production all around the world. The seeds of broomrapes germinate when they are exposed to germination stimulants, mainly strigolactones (SLs), released from the roots of any plant species; however, broomrapes parasitize only dicot plants. Therefore, monocots can be trap crops for broomrapes, as they induce seed germination but are not parasitized. In this study, we examined two European and one Japanese barley cultivar for their potential as trap crops for broomrapes. We found that the European cultivars, Sebastian and Golden Promise, are good potential trap crops, as they produce more SLs and exhibit higher mycorrhizal colonization rates as compared to the Japanese cultivar Shunrai.
  English, Scientific journal
  DOI：https://doi.org/10.1584/jpestics.D24-034
  DOI ID：10.1584/jpestics.D24-034, PubMed ID：39877875, PubMed Central ID：PMC11770136
• A Stereoselective Strigolactone Biosynthesis Catalyzed by a 2-Oxoglutarate-Dependent Dioxygenase in Sorghum.　　　　　　　　　　　　　　　
  Akiyoshi Yoda; Xiaonan Xie; Kaori Yoneyama; Kenji Miura; Christopher S P McErlean; Takahito Nomura
  Plant & cell physiology, Volume：64, Number：9, First page：1034, Last page：1045, Sep. 2023, [Reviewed], ［Domestic magazine］
  Seeds of root parasitic plants, Striga, Orobanche and Phelipanche spp., are induced to germinate by strigolactones (SLs) exudated from host roots. In Striga-resistant cultivars of Sorghum bicolor, the loss-of-function of the Low Germination Stimulant 1 (LGS1) gene changes the major SL from 5-deoxystrigol (5DS) to orobanchol, which has an opposite C-ring stereochemistry. The biosynthetic pathway of 5DS catalyzed by LGS1 has not been fully elucidated. Since other unknown regulators, in addition to LGS1 encoding a sulfotransferase, appear to be necessary for the stereoselective biosynthesis of 5DS, we examined Sobic.005G213500 (Sb3500), encoding a 2-oxoglutarate-dependent dioxygenase, as a candidate regulator, which is co-expressed with LGS1 and located 5'-upstream of LGS1 in the sorghum genome. When LGS1 was expressed with known SL biosynthetic enzyme genes including the cytochrome P450 SbMAX1a in Nicotiana benthamiana leaves, 5DS and its diastereomer 4-deoxyorobanchol (4DO) were produced in approximately equal amounts, while the production of 5DS was significantly larger than that of 4DO when Sb3500 was also co-expressed. We also confirmed the stereoselective 5DS production in an in vitro feeding experiment using synthetic chemicals with recombinant proteins expressed in Escherichia coli and yeast. This finding demonstrates that Sb3500 is a stereoselective regulator in the conversion of the SL precursor carlactone to 5DS, catalyzed by LGS1 and SbMAX1a, providing a detailed understanding of how different SLs are produced to combat parasitic weed infestations.
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcad060
  DOI ID：10.1093/pcp/pcad060, PubMed ID：37307421, PubMed Central ID：PMC10504574
• Whispers in the dark: Signals regulating underground plant–plant interactions
  Yoneyama, K.; Bennett, T.
  Current Opinion in Plant Biology, Volume：77, Sep. 2023, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1016/j.pbi.2023.102456
  DOI ID：10.1016/j.pbi.2023.102456, ISSN：1369-5266, ORCID：152547191, SCOPUS ID：85172448411
• Ancestral sequence reconstruction of the CYP711 family reveals functional divergence in strigolactone biosynthetic enzymes associated with gene duplication events in monocot grasses　　　　　　　　　　　　　　　
  Kaori Yoneyama
  New Phytologist, Volume：235, Number：5, First page：1900, Last page：1912, Sep. 2022, [Reviewed]
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/nph.18285
  DOI ID：10.1111/nph.18285, ISSN：0028-646X, ORCID：119226109
• Environmental strigolactone drives early growth responses to neighboring plants and soil volume in pea　　　　　　　　　　　　　　　
  Kaori Yoneyama
  Current Biology, Volume：32, Number：16, First page：3593, Last page：3600.e3, Aug. 2022
  Elsevier {BV}, English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2022.06.063
  DOI ID：10.1016/j.cub.2022.06.063, ISSN：0960-9822, ORCID：119226101
• Supra-organismal regulation of strigolactone exudation and plant development in response to rhizospheric cues in rice　　　　　　　　　　　　　　　
  Kaori Yoneyama
  Current Biology, Volume：32, Number：16, First page：3601, Last page：3608.e3, Aug. 2022, ［International magazine］
  Plants have evolved elaborate mechanisms to detect neighboring plants, which typically involve the perception of "cues" inadvertently produced by the neighbor.1 Strigolactones are hormonal signaling molecules2,3 that are also exuded into the rhizosphere by most flowering plant species to promote arbuscular mycorrhizal symbioses.4 Since flowering plants have an endogenous perception system for strigolactones,5 strigolactones are obvious candidates to act as a cue for neighbor presence, but have not been shown to act as such. To test this hypothesis in rice plants, we quantified two major strigolactones of rice plants, orobanchol and 4-deoxyorobanchol, in root exudates by using LC-MS/MS (MRM) and examined feedback regulation of strigolactone biosynthesis and changes in shoot branching phenotypes in rice plants grown at different densities in hydroponics and soil culture. We show that the presence of neighboring plants, or greater root volume, results in rapidly induced changes in strigolactone biosynthesis, sensitivity, and exudation and the subsequent longer-term changes in shoot architecture. These changes require intact strigolactone biosynthesis in neighboring plants and intact strigolactone signaling in focal plants. These results suggest that strigolactone biosynthesis and exudation in rice plants are driven by supra-organismal environmental strigolactone levels. Strigolactones thus act as a cue for neighbor presence in rice plants, but also seem to act as a more general root density-sensing mechanism in flowering plants that integrates soil volume and neighbor density and allows plants to adapt to the limitations of the rhizosphere.
  Elsevier {BV}, English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2022.06.047
  DOI ID：10.1016/j.cub.2022.06.047, ISSN：0960-9822, ORCID：119226086, PubMed ID：35839765
• Germination Stimulant Activity of Isothiocyanates on Phelipanche spp.　　　　　　　　　　　　　　　
  Hinako Miura; Ryota Ochi; Hisashi Nishiwaki; Satoshi Yamauchi; Xiaonan Xie; Hidemitsu Nakamura; Koichi Yoneyama; Kaori Yoneyama
  Plants (Basel, Switzerland), Volume：11, Number：5, Feb. 2022, ［International magazine］
  The root parasitic weed broomrapes, Phelipanche spp., cause severe damage to agriculture all over the world. They have a special host-dependent lifecycle and their seeds can germinate only when they receive chemical signals released from host roots. Our previous study demonstrated that 2-phenylethyl isothiocyanate is an active germination stimulant for P. ramosa in root exudates of oilseed rape. In the present study, 21 commercially available ITCs were examined for P. ramosa seed germination stimulation, and some important structural features of ITCs for exhibiting P. ramosa seed germination stimulation have been uncovered. Structural optimization of ITC for germination stimulation resulted in ITCs that are highly active to P. ramosa. Interestingly, these ITCs induced germination of P. aegyptiaca but not Orobanche minor or Striga hermonthica. P. aegyptiaca seeds collected from mature plants parasitizing different hosts responded to these ITCs with different levels of sensitivity. ITCs have the potential to be used as inducers of suicidal germination of Phelipanche seeds.
  English, Scientific journal
  DOI：https://doi.org/10.3390/plants11050606
  DOI ID：10.3390/plants11050606, PubMed ID：35270076, PubMed Central ID：PMC8912868
• Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum　　　　　　　　　　　　　　　
  Akiyoshi Yoda; Narumi Mori; Kohki Akiyama; Mayu Kikuchi; Xiaonan Xie; Kenji Miura; Kaori Yoneyama; Kanna Sato-Izawa; Shinjiro Yamaguchi; Koichi Yoneyama; David C. Nelson; Takahito Nomura
  NEW PHYTOLOGIST, Volume：232, Number：5, First page：1999, Last page：2010, Dec. 2021
  Root parasitic plants such as Striga, Orobanche, and Phelipanche spp. cause serious damage to crop production world-wide. Deletion of the Low Germination Stimulant 1 (LGS1) gene gives a Striga-resistance trait in sorghum (Sorghum bicolor). The LGS1 gene encodes a sulfotransferase-like protein, but its function has not been elucidated. Since the profile of strigolactones (SLs) that induce seed germination in root parasitic plants is altered in the lgs1 mutant, LGS1 is thought to be an SL biosynthetic enzyme. In order to clarify the enzymatic function of LGS1, we looked for candidate SL substrates that accumulate in the lgs1 mutants and performed in vivo and in vitro metabolism experiments. We found the SL precursor 18-hydroxycarlactonoic acid (18-OH-CLA) is a substrate for LGS1. CYP711A cytochrome P450 enzymes (SbMAX1 proteins) in sorghum produce 18-OH-CLA. When LGS1 and SbMAX1 coding sequences were co-expressed in Nicotiana benthamiana with the upstream SL biosynthesis genes from sorghum, the canonical SLs 5-deoxystrigol and 4-deoxyorobanchol were produced. This finding showed that LGS1 in sorghum uses a sulfo group to catalyze leaving of a hydroxyl group and cyclization of 18-OH-CLA. A similar SL biosynthetic pathway has not been found in other plant species.
  WILEY, English, Scientific journal
  DOI：https://doi.org/10.1111/nph.17737
  DOI ID：10.1111/nph.17737, ISSN：0028-646X, eISSN：1469-8137, Web of Science ID：WOS:000702893700001
• Structure-activity relationship of the aromatic moiety of 6-substituted 5,6-dihydro-2-pyrone to find the novel compound showing higher plant growth inhibitory activity.　　　　　　　　　　　　　　　
  Ryota Ochi; Kaori Yoneyama; Hisashi Nishiwaki; Satoshi Yamauchi
  Bioscience, biotechnology, and biochemistry, Volume：86, Number：2, First page：165, Last page：169, Oct. 2021, ［International magazine］
  In the course of our research on the structure-activity relationship of 5,6-dihydro-2H-pyran-2-one, (S)-6-. We also identified endogenous CLA and its methyl ester [methyl carlactonoate (MeCLA)] in Arabidopsis plants using LC-MS/MS. Although an exogenous application of either CLA or MeCLA suppressed the growth of lateral inflorescences of the max1 mutant, MeCLA, but not CLA, interacted with Arabidopsis thaliana DWARF14 (AtD14) protein, a putative SL receptor, as shown by differential scanning fluorimetry and hydrolysis activity tests. These results indicate that not only known SLs but also MeCLA are biologically active in inhibiting shoot branching in Arabidopsis.
  NATL ACAD SCIENCES, English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1410801111
  DOI ID：10.1073/pnas.1410801111, ISSN：0027-8424, PubMed ID：25425668, Web of Science ID：WOS:000346366500087
• Avenaol, a germination stimulant for root parasitic plants from Avena strigosa　　　　　　　　　　　　　　　
  Hyun Ii Kim; Takaya Kisugi; Pichit Khetkam; Xiaonan Xie; Kaori Yoneyama; Kenichi Uchida; Takao Yokota; Takahito Nomura; Christopher S. P. McErlean; Koichi Yoneyama
  PHYTOCHEMISTRY, Volume：103, First page：85, Last page：88, Jul. 2014, [Reviewed]
  Root exudates from the allelopathic plant, black oat (Avena strigosa Schreb.), were found to contain at least six different germination stimulants for root parasitic plants, but no known strigolactones (SLs). One of these germination stimulants was purified and named avenaol. Its HR-ESI-TOFMS analysis indicated that the molecular formula of avenaol is C20H24O7, and thus it contains an additional carbon compared with known C-19-SLs. Its structure was determined as 5-((E)-(5-(3-hydroxy-1,5,5-trimethy-2-oxobicyclo[4.1.0]heptan-7-yl)-2-oxodihydrofuran-3(2H)-ylidene)methoxy)-3-methylfuran-2(5H)-one, by 1D and 2D NMR spectroscopy, and ESI- and El-MS spectrometry. Although avenaol contains the C-D moiety, the common structural feature for all known SLs, it lacks the B ring and has an additional carbon atom between the A and C rings. Avenaol is a potent germination stimulant of Phelipanche ramosa seeds, but only a weak stimulant for seeds of Striga hermonthica and Orobanche minor. (C) 2014 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, English, Scientific journal
  DOI：https://doi.org/10.1016/j.phytochem.2014.03.030
  DOI ID：10.1016/j.phytochem.2014.03.030, ISSN：0031-9422, PubMed ID：24768285, Web of Science ID：WOS:000337777800010
• Nitrogen and phosphorus fertilization negatively affects strigolactone production and exudation in sorghum　　　　　　　　　　　　　　　
  Kaori Yoneyama; Xiaonan Xie; Takaya Kisugi; Takahito Nomura; Koichi Yoneyama
  PLANTA, Volume：238, Number：5, First page：885, Last page：894, Nov. 2013, [Reviewed]
  Strigolactones (SLs) are essential host recognition signals for both root parasitic plants and arbuscular mycorrhizal fungi, and SLs or their metabolites function as a novel class of plant hormones regulating shoot and root architecture. Our previous study indicated that nitrogen (N) deficiency as well as phosphorus (P) deficiency in sorghum enhanced root content and exudation of 5-deoxystrigol, one of the major SLs produced by sorghum. In the present study, we examined how N and P fertilization affects SL production and exudation in sorghum plants subjected to short- (5 days) or long-term (10 days) N or P deficiency and demonstrated their common and distinct features. The root contents and exudation of SLs in the N- or P-deficient sorghum plants grown for 6, 12 or 24 h with or without N or P fertilization were quantified by LC-MS/MS. In general, without fertilization, root contents and exudation of SLs stayed at similar levels at 6 and 12 h and then significantly increased at 24 h. The production of SLs responded more quickly to P fertilization than the secretion of SLs, while regulation of SL secretion began earlier after N fertilization. It is suggested that sorghum plants regulate SL production and exudation when they are subjected to nutrient deficiencies depending on the type of nutrient and degree of deficiency.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-013-1943-8
  DOI ID：10.1007/s00425-013-1943-8, ISSN：0032-0935, eISSN：1432-2048, PubMed ID：23925853, Web of Science ID：WOS:000326073800007
• Relationship between gene responses and symptoms induced by Rice grassy stunt virus　　　　　　　　　　　　　　　
  Kouji Satoh; Kaori Yoneyama; Hiroaki Kondoh; Takumi Shimizu; Takahide Sasaya; Il-Ryong Choi; Koichi Yoneyama; Toshihiro Omura; Shoshi Kikuchi
  FRONTIERS IN MICROBIOLOGY, Volume：4, First page：313, Oct. 2013, [Reviewed]
  Rice grassy stunt virus (RGSV) is a serious threat to rice production in Southeast Asia. RGSV is a member of the genus Tenuivirus, and it induces leaf yellowing, stunting, and excess tillering on rice plants. Here we examined gene responses of rice to RGSV infection to gain insight into the gene responses which might be associated with the disease symptoms. The results indicated that (1) many genes related to cell wall synthesis and chlorophyll synthesis were predominantly suppressed by RGSV infection; (2) RGSV infection induced genes associated with tillering process; (3) RGSV activated genes involved in inactivation of gibberellic acid and indole-3-acetic acid; and (4) the genes for strigolactone signaling were suppressed by RGSV. These results suggest that these gene responses to RGSV infection account for the excess tillering specific to RGSV infection as well as other symptoms by RGSV, such as stunting and leaf chlorosis.
  FRONTIERS RESEARCH FOUNDATION, English, Scientific journal
  DOI：https://doi.org/10.3389/fmicb.2013.00313
  DOI ID：10.3389/fmicb.2013.00313, ISSN：1664-302X, PubMed ID：24151491, Web of Science ID：WOS:000331529300001
• Chemistry of Strigolactones: Why and How do Plants Produce so Many Strigolactones?　　　　　　　　　　　　　　　
  Koichi Yoneyama; Takaya Kisugi; Xiaonan Xie; Kaori Yoneyama
  Molecular Microbial Ecology of the Rhizosphere, Volume：1, First page：373, Last page：379, Mar. 2013, [Reviewed]
  Strigolactones (SLs) are plant secondary metabolites derived from carotenoids. SLs were originally isolated as germination stimulants for root parasitic plants witchweeds (Striga spp.) and broomrapes (Orobanche and Phelipanche spp.), and then found as host recognition signals for arbuscular mycorrhizal (AM) fungi with which >
  80% land plants form a symbiotic relationship. In addition to these functions of SLs in the rhizosphere, SLs have been shown to act as a novel class of plant hormone regulating shoot and root architecture, photomorphogenesis, and secondary growth. In this chapter, we will focus on diverse chemistry of SLs and discuss why and how do plants produce so many strigolactones. © 2013 John Wiley &
  Sons, Ltd.
  John Wiley and Sons, English, In book
  DOI：https://doi.org/10.1002/9781118297674.ch34
  DOI ID：10.1002/9781118297674.ch34, SCOPUS ID：84886561603
• Strigone, isolation and identification as a natural strigolactone from Houttuynia cordata　　　　　　　　　　　　　　　
  Takaya Kisugi; Xiaonan Xie; Hyun Il Kim; Kaori Yoneyama; Aika Sado; Kohki Akiyama; Hideo Hayashi; Kenichi Uchida; Takao Yokota; Takahito Nomura; Koichi Yoneyama
  PHYTOCHEMISTRY, Volume：87, First page：60, Last page：64, Mar. 2013, [Reviewed]
  (+)-Strigone was described earlier in a paper on isolation of strigol and then recently examined for hyphal branching activity in arbuscular mycorrhizal fungi as a strigolactone. Herein, it was isolated from root exudates of Houttuynia cordata, and its structure was confirmed by direct comparison with synthetic standards in LC-MS/MS, GC-MS, and H-1 and C-13 NMR analyses. The stereochemistry of strigone was determined by comparing the CD spectra and RRt in chiral LC-MS/MS with those of synthetic (+)-strigone and ()-strigone. Four stereoisomers of strigone exhibited clearly different levels of stimulation activity on the seeds of three root parasitic plants, Orobanche minor, Phelipanche ramosa, and Striga hermonthica. (+)-Strigone was a highly potent germination stimulant on S. hermonthica and also on P. ramosa, but less active than ent-2'-epi-strigone on G. minor. In addition to strigone, H. cordata was found to produce strigol, sorgomol, and 5-deoxystrigol, indicating that this plant produces mainly strigol-type strigolactones derived from 5-deoxystrigol. (C) 2012 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, English, Scientific journal
  DOI：https://doi.org/10.1016/j.phytochem.2012.11.013
  DOI ID：10.1016/j.phytochem.2012.11.013, ISSN：0031-9422, PubMed ID：23290861, Web of Science ID：WOS:000315078900007
• Strigolactones: Internal and external signals in plant symbioses?　　　　　　　　　　　　　　　
  Eloise Foo; Kaori Yoneyama; Cassandra Hugill; Laura J. Quittenden; James B. Reid
  Plant Signaling and Behavior, Volume：8, Number：3, First page：e23168.4, Mar. 2013, [Reviewed]
  As the newest plant hormone, strigolactone research is undergoing an exciting expansion. In less than five years, roles for strigolactones have been defined in shoot branching, secondary growth, root growth and nodulation, to add to the growing understanding of their role in arbuscular mycorrhizae and parasitic weed interactions.1 Strigolactones are particularly fascinating as signaling molecules as they can act both inside the plant as an endogenous hormone and in the soil as a rhizosphere signal.2-4 Our recent research has highlighted such a dual role for strigolactones, potentially acting as both an endogenous and exogenous signal for arbuscular mycorrhizal development.5 There is also significant interest in examining strigolactones as putative regulators of responses to environmental stimuli, especially the response to nutrient availability, given the strong regulation of strigolactone production by nitrate and phosphate observed in many species.5,6 In particular, the potential for strigolactones to mediate the ecologically important response of mycorrhizal colonization to phosphate has been widely discussed. However, using a mutant approach we found that strigolactones are not essential for phosphate regulation of mycorrhizal colonization or nodulation.5 This is consistent with the relatively mild impairment of phosphate control of seedling root growth observed in Arabidopsis strigolactone mutants.7 This contrasts with the major role for strigolactones in phosphate control of shoot branching of rice and Arabidopsis8,9 and indicates that the integration of strigolactones into our understanding of nutrient response will be complex. New data presented here, along with the recent discovery of phosphate specific CLE peptides,10 indicates a potential role for PsNARK, a component of the autoregulation of nodulation pathway, in phosphate control of nodulation. © 2013 Landes Bioscience.
  English, Scientific journal
  DOI：https://doi.org/10.4161/psb.23168
  DOI ID：10.4161/psb.23168, ISSN：1559-2316, PubMed ID：23299321, SCOPUS ID：84874695796
• Strigolactones and biological activity　　　　　　　　　　　　　　　
  Koichi Yoneyama; Xiaonan Xie; Kaori Yoneyama
  Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes, First page：3583, Last page：3604, Jan. 2013, [Reviewed]
  Strigolactones (SLs) are carotenoid-derived plant terpenes which function in the rhizosphere and in planta. In the rhizosphere, SLs induce seed germination in root parasitic plants and hyphal branching in arbuscular mycorrhizal (AM) fungi, therefore mediating both parasitic and symbiotic interactions with SL-producing plants and root parasitic plants and AM fungi, respectively. In planta, SLs are a group of plant hormones regulating shoot as well as root architecture. In this chapter, chemistry, biosynthesis and its regulation, analytical methods, and biological functions of SLs are discussed along with their potential agronomical uses.
  Springer Berlin Heidelberg, English, In book
  DOI：https://doi.org/10.1007/978-3-642-22144-6_155
  DOI ID：10.1007/978-3-642-22144-6_155, SCOPUS ID：84924360213
• Strigolactones and the Regulation of Pea Symbioses in Response to Nitrate and Phosphate Deficiency　　　　　　　　　　　　　　　
  Eloise Foo; Kaori Yoneyama; Cassandra J. Hugill; Laura J. Quittenden; James B. Reid
  MOLECULAR PLANT, Volume：6, Number：1, First page：76, Last page：87, Jan. 2013, [Reviewed]
  New roles for the recently identified group of plant hormones, the strigolactones, are currently under active investigation. One of their key roles is to regulate plant symbioses. These compounds act as a rhizosphere signal in arbuscular mycorrhizal symbioses and as a positive regulator of nodulation in legumes. The phosphorous and nitrogen status of the soil has emerged as a powerful regulator of strigolactone production. However, until now, the potential role of strigolactones in regulating mycorrhizal development and nodulation in response to nutrient deficiency has not been proven. In this paper, the role of strigolactone synthesis and response in regulating these symbioses is examined in pea (Pisum sativum L.). Pea is well suited to this study, since there is a range of well-characterized strigolactone biosynthesis and response mutants that is unique amongst legumes. Evidence is provided for a novel endogenous role for strigolactone response within the root during mycorrhizal development, in addition to the action of strigolactones on the fungal partner. The strigolactone response pathway that regulates mycorrhizal development also appears to differ somewhat from the response pathway that regulates nodulation. Finally, studies with strigolactone-deficient pea mutants indicate that, despite strong regulation of strigolactone production by both nitrogen and phosphate, strigolactones are not required to regulate these symbioses in response to nutrient deficiency.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/mp/sss115
  DOI ID：10.1093/mp/sss115, ISSN：1674-2052, eISSN：1752-9867, PubMed ID：23066094, Web of Science ID：WOS:000314117100011
• Confirming Stereochemical Structures of Strigolactones Produced by Rice and Tobacco　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Takaya Kisugi; Kenichi Uchida; Seisuke Ito; Kohki Akiyama; Hideo Hayashi; Takao Yokota; Takahito Nomura; Koichi Yoneyama
  MOLECULAR PLANT, Volume：6, Number：1, First page：153, Last page：163, Jan. 2013, [Reviewed]
  Major strigolactones produced by rice (Oryza sativa L.) and tobacco (Nicotiana tabacum L.) were purified and their stereochemical structures were determined definitely by comparing with optically pure synthetic standards for spectroscopic data.Major strigolactones (SLs) produced by rice (Oryza sativa L. cv. Nipponbare) and tobacco (Nicotiana tabacum L. cv. Michinoku No. 1) were purified and their stereochemical structures were determined by comparing with optically pure synthetic standards for their NMR and CD data and retention times and mass fragmentations in ESILC/MS and GCMS. SLs purified from root exudates of rice plants were orobanchol, orobanchyl acetate, and ent-2'-epi-5-deoxystrigol. In addition to these SLs, 7-oxoorobanchyl acetate and the putative three methoxy-5-deoxystrigol isomers were detected by LCMS/MS. The production of 7-oxoorobanchyl acetate seemed to occur in the early growth stage, as it was detected only in the root exudates collected during the first week of incubation. The root exudates of tobacco contained at least 11 SLs, including solanacol, solanacyl acetate, orobanchol, ent-2'-epi-orobanchol, orobanchyl acetate, ent-2'-epi-orobanchyl acetate, 5-deoxystrigol, ent-2'-epi-5-deoxystrigol, and three isomers of putative didehydro-orobanchol whose structures remain to be clarified. Furthermore, two sorgolactone isomers but not sorgolactone were detected as minor SLs by LCMS/MS analysis. It is intriguing to note that rice plants produced only orobanchol-type SLs, derived from ent-2'-epi-5-deoxystrigol, but both orobanchol-type and strigol-type SLs, derived from 5-deoxystrigol were detected in tobacco plants.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/mp/sss139
  DOI ID：10.1093/mp/sss139, ISSN：1674-2052, PubMed ID：23204500, Web of Science ID：WOS:000314117100017
• Germination Stimulants of Phelipanche ramosa in the Rhizosphere of Brassica napus Are Derived from the Glucosinolate Pathway　　　　　　　　　　　　　　　
  Bathilde Auger; Jean-Bernard Pouvreau; Karinne Pouponneau; Kaori Yoneyama; Gregory Montiel; Bruno Le Bizec; Koichi Yoneyama; Philippe Delavault; Regine Delourme; Philippe Simier
  MOLECULAR PLANT-MICROBE INTERACTIONS, Volume：25, Number：7, First page：993, Last page：1004, Jul. 2012, [Reviewed]
  Phelipanche ramosa is a major parasitic weed of Brassica napus. The first step in a host-parasitic plant interaction is stimulation of parasite seed germination by compounds released from host roots. However, germination stimulants produced by B. napus have not been identified yet. In this study, we characterized the germination stimulants that accumulate in B. napus roots and are released into the rhizosphere. Eight glucosinolate-breakdown products were identified and quantified in B. napas roots by gas chromatography mass spectrometry. Two (3-phenylpropanenitrile and 2-phenylethyl isothiocyanate [2-PEITC1) were identified in the B. napus rhizosphere. Among glucosinolate-breakdown products, P ramosa germination was strongly and specifically triggered by isothiocyanates, indicating that 2-PEITC, in particular, plays a key role in the B. napus P ramosa interaction. Known strigolactones were not detected by ultraperformance liquid chromatography tandem mass spectrometry, and seed of Phelipanche and Orobanche spp. that respond to strigolactones but not to isothiocyanates did not germinate in the rhizosphere of B. napus. Furthermore, both wild-type and strigolactone biosynthesis mutants of Arabidopsis thaliana Atccd7 and Atccd8 induced similar levels of P ramosa seed germination, suggesting that compounds other than strigolactone function as germination stimulants for P ramosa in other Brassicaceae spp. Our results open perspectives on the high adaptation potential of root-parasitic plants under host-driven selection pressures.
  AMER PHYTOPATHOLOGICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1094/MPMI-01-12-0006-R
  DOI ID：10.1094/MPMI-01-12-0006-R, ISSN：0894-0282, eISSN：1943-7706, PubMed ID：22414435, Web of Science ID：WOS:000305503700014
• How do nitrogen and phosphorus deficiencies affect strigolactone production and exudation?　　　　　　　　　　　　　　　
  Kaori Yoneyama; Xiaonan Xie; Hyun Il Kim; Takaya Kisugi; Takahito Nomura; Hitoshi Sekimoto; Takao Yokota; Koichi Yoneyama
  PLANTA, Volume：235, Number：6, First page：1197, Last page：1207, Jun. 2012, [Reviewed]
  Plants exude strigolactones (SLs) to attract symbiotic arbuscular mycorrhizal fungi in the rhizosphere. Previous studies have demonstrated that phosphorus (P) deficiency, but not nitrogen (N) deficiency, significantly promotes SL exudation in red clover, while in sorghum not only P deficiency but also N deficiency enhances SL exudation. There are differences between plant species in SL exudation under P- and N-deficient conditions, which may possibly be related to differences between legumes and non-legumes. To investigate this possibility in detail, the effects of N and P deficiencies on SL exudation were examined in Fabaceae (alfalfa and Chinese milk vetch), Asteraceae (marigold and lettuce), Solanaceae (tomato), and Poaceae (wheat) plants. In alfalfa as expected, and unexpectedly in tomato, only P deficiency promoted SL exudation. In contrast, in Chinese milk vetch, a leguminous plant, and in the other non-leguminous plants examined, N deficiency as well as P deficiency enhanced SL exudation. Distinct reductions in shoot P levels were observed in plants grown under N deficiency, except for tomato, in which shoot P level was increased by N starvation, suggesting that the P status of the shoot regulates SL exudation. There seems to be a correlation between shoot P levels and SL exudation across the species/families investigated.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-011-1568-8
  DOI ID：10.1007/s00425-011-1568-8, ISSN：0032-0935, eISSN：1432-2048, PubMed ID：22183123, Web of Science ID：WOS:000304621200008
• Characterization of strigolactones exuded by Asteraceae plants　　　　　　　　　　　　　　　
  Kaori Yoneyama; Xiaonan Xie; Takaya Kisugi; Takahito Nomura; Hitoshi Sekimoto; Takao Yokota; Koichi Yoneyama
  PLANT GROWTH REGULATION, Volume：65, Number：3, First page：495, Last page：504, Dec. 2011, [Reviewed]
  Strigolactones (SLs), originally characterized as germination stimulants for root parasitic weeds, are now recognized as hyphal branching factors for symbiotic arbuscular mycorrhizal fungi and as a novel class of plant hormones inhibiting shoot branching. In the present study, SLs in root exudates of 13 Asteraceae plants including crops, a weed, and ornamental plants were characterized. High performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) analyses revealed that all the Asteraceae plants examined exuded known SLs and, except for sunflower (Helianthus annuus), high germination stimulant activities at retention times corresponding to these SLs were confirmed. The two major SLs exuded by these Asteraceae plants were orobanchyl acetate and orobanchol. 5-Deoxystrigol and 7-hydroxyorobanchyl acetate were detected in root exudates from several Asteraceae species examined in this study.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s10725-011-9620-z
  DOI ID：10.1007/s10725-011-9620-z, ISSN：0167-6903, eISSN：1573-5087, Web of Science ID：WOS:000297621200007
• Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens　　　　　　　　　　　　　　　
  Helene Proust; Beate Hoffmann; Xiaonan Xie; Kaori Yoneyama; Didier G. Schaefer; Koichi Yoneyama; Fabien Nogue; Catherine Rameau
  DEVELOPMENT, Volume：138, Number：8, First page：1531, Last page：1539, Apr. 2011, [Reviewed]
  Strigolactones are a novel class of plant hormones controlling shoot branching in seed plants. They also signal host root proximity during symbiotic and parasitic interactions. To gain a better understanding of the origin of strigolactone functions, we characterised a moss mutant strongly affected in strigolactone biosynthesis following deletion of the CAROTENOID CLEAVAGE DIOXYGENASE 8 (CCD8) gene. Here, we show that wild-type Physcomitrella patens produces and releases strigolactones into the medium where they control branching of protonemal filaments and colony extension. We further show that Ppccd8 mutant colonies fail to sense the proximity of neighbouring colonies, which in wild-type plants causes the arrest of colony extension. The mutant phenotype is rescued when grown in the proximity of wild-type colonies, by exogenous supply of synthetic strigolactones or by ectopic expression of seed plant CCD8. Thus, our data demonstrate for the first time that Bryophytes (P. patens) produce strigolactones that act as signalling factors controlling developmental and potentially ecophysiological processes. We propose that in P. patens, strigolactones are reminiscent of quorum-sensing molecules used by bacteria to communicate with one another.
  COMPANY OF BIOLOGISTS LTD, English, Scientific journal
  DOI：https://doi.org/10.1242/dev.058495
  DOI ID：10.1242/dev.058495, ISSN：0950-1991, PubMed ID：21367820, Web of Science ID：WOS:000288649400009
• Strigolactone Deficiency Confers Resistance in Tomato Line SL-ORT1 to the Parasitic Weeds Phelipanche and Orobanche spp.　　　　　　　　　　　　　　　
  Evgenia Dor; Koichi Yoneyama; Smadar Wininger; Yoram Kapulnik; Kaori Yoneyama; Hinanit Koltai; Xiaonan Xie; Joseph Hershenhorn
  PHYTOPATHOLOGY, Volume：101, Number：2, First page：213, Last page：222, Feb. 2011, [Reviewed]
  The parasitic flowering plants of the genera Orobanche and Phelipanche (broomrape species) are obligatory chlorophyll-lacking root-parasitic weeds that infect dicotyledonous plants and cause heavy economic losses in a wide variety of plant species in warm-temperate and subtropical regions. One of the most effective strategies for broomrape control is crop breeding for broomrape resistance. Previous efforts to find natural broomrape-resistant tomato (Solanum lycopersicon) genotypes were unsuccessful, and no broomrape resistance was found in any wild tomato species. Recently, however, the fast-neutron-mutagenized tomato mutant SL-ORT1 was found to be highly resistant to various Phelipanche and Orobanche spp. Nevertheless, SL-ORT1 plants were parasitized by Phelipanche aegyptiaca if grown in pots together with the susceptible tomato cv. M-82. In the present study, no toxic activity or inhibition of Phelipanche seed germination could be detected in the SL-ORT1 root extracts. SL-ORT1 roots did not induce Phelipanche seed germination in pots but they were parasitized, at the same level as M-82, after application of the synthetic germination stimulant GR24 to the rhizosphere. Whereas liquid chromatography coupled to tandem mass spectrometry analysis of root exudates of M-82 revealed the presence of the strigolactones orobanchol, solanacol, and didehydro-orobanchol isomer, these compounds were not found in the exudates of SL-ORT1. It can be concluded that SL-ORT1 resistance results from its inability to produce and secrete natural germination stimulants to the rhizosphere.
  AMER PHYTOPATHOLOGICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1094/PHYTO-07-10-0184
  DOI ID：10.1094/PHYTO-07-10-0184, ISSN：0031-949X, PubMed ID：20942651, Web of Science ID：WOS:000286644800005
• Strigolactones as Germination Stimulants for Root Parasitic Plants　　　　　　　　　　　　　　　
  Koichi Yoneyama; Ayman A. Awad; Xiaonan Xie; Kaori Yoneyama; Yasutomo Takeuchi
  PLANT AND CELL PHYSIOLOGY, Volume：51, Number：7, First page：1095, Last page：1103, Jul. 2010, [Reviewed]
  Witchweeds (Striga spp.) and broomrapes (Orobanche and Phelipanche spp.) are the two most devastating root parasitic plants belonging to the family Orobanchaceae and are causing enormous crop losses throughout the world. Seeds of these root parasites will not germinate unless they are exposed to chemical stimuli, 'germination stimulants' produced by and released from plant roots. Most of the germination stimulants identified so far are strigolactones (SLs), which also function as host recognition signals for arbuscular mycorrhizal fungi and a novel class of plant hormones inhibiting shoot branching. In this review, we focus on SLs as germination stimulants for root parasitic plants. In addition, we discuss how quantitative and qualitative differences in SL exudation among sorghum cultivars influence their susceptibility to Striga.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcq055
  DOI ID：10.1093/pcp/pcq055, ISSN：0032-0781, PubMed ID：20403809, Web of Science ID：WOS:000280318500002
• A tomato strigolactone-impaired mutant displays aberrant shoot morphology and plant interactions　　　　　　　　　　　　　　　
  Hinanit Koltai; Sivarama P. LekKala; Chaitali Bhattacharya; Einav Mayzlish-Gati; Nathalie Resnick; Smadar Wininger; Evgenya Dor; Kaori Yoneyama; Koichi Yoneyama; Joseph Hershenhorn; Daniel M. Joel; Yoram Kapulnik
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：61, Number：6, First page：1739, Last page：1749, Apr. 2010, [Reviewed]
  Strigolactones are considered a new group of plant hormones. Their role as modulators of plant growth and signalling molecules for plant interactions first became evident in Arabidopsis, pea, and rice mutants that were flawed in strigolactone production, release, or perception. The first evidence in tomato (Solanum lycopersicon) of strigolactone deficiency is presented here. Sl-ORT1, previously identified as resistant to the parasitic plant Orobanche, had lower levels of arbuscular mycorrhizal fungus (Glomus intraradices) colonization, possibly as a result of its reduced ability to induce mycorrhizal hyphal branching. Biochemical analysis of mutant root extracts suggested that it produces only minute amounts of two of the tomato strigolactones: solanacol and didehydro-orobanchol. Accordingly, the transcription level of a key enzyme (CCD7) putatively involved in strigolactone synthesis in tomato was reduced in Sl-ORT1 compared with the wild type (WT). Sl-ORT1 shoots exhibited increased lateral shoot branching, whereas exogenous application of the synthetic strigolactone GR24 to the mutant restored the WT phenotype by reducing the number of lateral branches. Reduced lateral shoot branching was also evident in grafted plants which included a WT interstock, which was grafted between the mutant rootstock and the scion. In roots of these grafted plants, the CCD7 transcription level was not significantly induced, nor was mycorrhizal sensitivity restored. Hence, WT-interstock grafting, which restores mutant shoot morphology to WT, does not restore mutant root properties to WT. Characterization of the first tomato strigolactone-deficient mutant supports the putative general role of strigolactones as messengers of suppression of lateral shoot branching in a diversity of plant species.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erq041
  DOI ID：10.1093/jxb/erq041, ISSN：0022-0957, PubMed ID：20194924, Web of Science ID：WOS:000276735300015
• The Strigolactone Story　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Koichi Yoneyama
  ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 48, Volume：48, First page：93, Last page：117, 2010, [Reviewed]
  Strigolactones (SLs) were originally isolated from plant root exudates as germination stimulants for root parasitic plants of the family Orobanchaceae, including witchweeds (Striga spp.), broomrapes (Orobanche and Phelipanche spp.), and Alectra spp., and so were regarded as detrimental to the producing plants. Their role as indispensable chemical signals for root colonization by symbiotic arbuscular mycorrhizal fungi was subsequently unveiled, and SLs then became recognized as beneficial plant metabolites. In addition to these functions in the rhizosphere, it has been recently shown that SLs or their metabolites are a novel class of plant hormones that inhibit shoot branching. Furthermore, SLs are suggested to have other biological functions in rhizosphere communications and in plant growth and development.
  ANNUAL REVIEWS, English, In book
  DOI：https://doi.org/10.1146/annurev-phyto-073009-114453
  DOI ID：10.1146/annurev-phyto-073009-114453, ISSN：0066-4286, PubMed ID：20687831, Web of Science ID：WOS:000282062400005
• 7-Oxoorobanchyl Acetate and 7-Oxoorobanchol as Germination Stimulants for Root Parasitic Plants from Flax (Linum usitatissimum)　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Jun-ya Kurita; Yuta Harada; Yoichi Yamada; Yasutomo Takeuchi; Koichi Yoneyama
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：73, Number：6, First page：1367, Last page：1370, Jun. 2009, [Reviewed]
  Germination stimulants for root parasitic plants produced by flax (Linum usitatissimum L.) were purified and characterized. The root exudate of flax contained at least 8 active fractions, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography mass spectrometry (GC-MS) analyses suggested that there were 6 strigolactones. Two of them were identified as orobanchol and orobanchyl acetate by comparing NMR and GC-MS and LC-MS/MS data with those of synthetic standards. One of the two novel strigolactones was purified and determined as 7-oxoorobanchyl acetate [((3aS,4S,8bSE)-8,8-dimethyl-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2,7- dioxo-3,3a,4,5,6,7,8,8b-octahydro-2H-indeno[1,2-b]furan-4-yl acetate) by 1D and 2D NMR spectroscopic, and ESI- and EI-MS spectrometric analyses. The other one was also purified and identified as 7-oxoorobanchol. The remaining two compounds could not been characterized due to their scarcity.
  TAYLOR & FRANCIS LTD, English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.90021
  DOI ID：10.1271/bbb.90021, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：19502732, Web of Science ID：WOS:000267872100021
• Strigolactones: structures and biological activities　　　　　　　　　　　　　　　
  Koichi Yoneyama; Xiaonan Xie; Kaori Yoneyama; Yasutomo Takeuchi
  PEST MANAGEMENT SCIENCE, Volume：65, Number：5, First page：467, Last page：470, May 2009, [Reviewed]
  Strigolactones released from plant roots induce seed germination of root parasitic weeds, witchweeds (Striga spp.) and broomrapes (Orobanche spp.), and hyphal branching of symbiotic arbuscular mycorrhizal (AM) fungi. In addition to these functions in the rhizosphere, strigolactones have recently been shown to be a novel class of plant hormones regulating shoot outgrowth. The natural strigolactones identified so far have the common C-D ring moiety, which is thought to be the essential structure for exhibiting biological activity. The introduction of substitutions on the A-B ring moiety of 5-deoxystrigol, the basic strigolactone, affords various strigolactones, e.g. hydroxylation on C-4, C-5 and C-9 leads to orobanchol, strigol and sorgomol respectively. Then, acetylation and probably other derivatisations of these hydroxy-strigolactones would occur. Although the C-2'-(R) stereochemistry was thought to be an important structural feature for potent germination stimulation activity, 2'-epi-strigolactones were found in root exudates of tobacco, rice, pea and other plant species, indicating that at least some plants produce both epimers. (C) 2009 Society of Chemical Industry
  WILEY-BLACKWELL, English
  DOI：https://doi.org/10.1002/ps.1726
  DOI ID：10.1002/ps.1726, ISSN：1526-498X, PubMed ID：19222028, Web of Science ID：WOS:000265726800004
• Fabacyl acetate, a germination stimulant for root parasitic plants from Pisum sativum　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Yuta Harada; Norio Fusegi; Yoichi Yamada; Satoshi Ito; Takao Yokota; Yasutomo Takeuchi; Koichi Yoneyama
  PHYTOCHEMISTRY, Volume：70, Number：2, First page：211, Last page：215, Jan. 2009, [Reviewed]
  A germination stimulant, fabacyl acetate. was purified from root exudates of pea (Pisum sativum L.) and its structure was determined as ent-2'-epi-4a,8a-epoxyorobanchyl acetate [(3aR,4R,4aR,8bS,E)-4a,8a-epoxy-8,8-dimethyl-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2-oxo- 3,3a,4,5,6,7,8,8b-decahydro-2H-indeno[1,2-b]furan-4-yl acetate], by 1D and 2D NMR spectroscopic, ESI-and E1-MS spectrometric, X-ray crystallographic analyses, and by comparing the H-1 NMR spectroscopic data and relative retention times (RRt) in LC-MS and CC-MS with those of synthetic standards prepared from (+)-orobanchol and (+)-2'-epiorobanchol. The H-1 NMR spectroscopic data and RRt of fabacyl acetate were identical with those of an isomer prepared from (+)-2'-epiorobanchol except for the opposite sign in CD spectra. This is the first natural ent-strigolactone containing an epoxide group. Fabacyl acetate was previously detected in root exudates of other Fabaceae plants including faba bean (Vicia faba Q and alfalfa (Medicago sativa L). (C) 2008 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, English, Scientific journal
  DOI：https://doi.org/10.1016/j.phytochem.2008.12.013
  DOI ID：10.1016/j.phytochem.2008.12.013, ISSN：0031-9422, PubMed ID：19155028, Web of Science ID：WOS:000264584000007
• Sorgomol, germination stimulant for root parasitic plants, produced by Sorghum bicolor　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Dai Kusumoto; Yoichi Yamada; Yasutomo Takeuchi; Yukihiro Sugimoto; Koichi Yoneyama
  Tetrahedron Letters, Volume：49, Number：13, First page：2066, Last page：2068, Mar. 2008, [Reviewed]
  A novel strigolactone sorgomol, germination stimulant for root parasitic plants Striga and Orobanche, was isolated and structure was elucidated. Sorgomol was more active on Striga than on Orobanche and may be the immediate precursor of sorgolactone in the biosynthetic pathway of strigolactones. © 2008 Elsevier Ltd. All rights reserved.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.tetlet.2008.01.131
  DOI ID：10.1016/j.tetlet.2008.01.131, ISSN：0040-4039, SCOPUS ID：39649111519, Web of Science ID：WOS:000254446000006
• Isolation and identification of alectrol as (+)-orobanchyl acetate, a germination stimulant for root parasitic plants　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Dai Kusumoto; Yolchi Yamada; Takao Yokota; Yasutomo Takeuchi; Koichi Yoneyama
  PHYTOCHEMISTRY, Volume：69, Number：2, First page：427, Last page：431, Jan. 2008, [Reviewed]
  Alectrol, a germination stimulant for root parasitic plants, was purified from root exudates of red clover (Trifolium pratense L.) and identified as a strigolactone, (+)-orobanchyl acetate [(3aS,4S,8bS,E)-8,8-dimethyl-3-(((R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yloxy)methylene)-2-oxo-3,3a,4,5,6,7,8,8b-octahydro-2H-indeno[1,2-b]furan-4-yl acetate], by ID and 2D NMR spectroscopy and ESI- and EI-MS spectrometry. Orobanchyl acetate afforded an [M-42](+) ion in EI-MS and thus had been recognized as an isomer of strigol. Orobanchyl acetate was detected in root exudates of soybean (Glycine max L.) and cowpea (Vigina unguiculata L.) along with orobanchol. (C) 2007 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, English, Scientific journal
  DOI：https://doi.org/10.1016/j.phytochem.2007.07.017
  DOI ID：10.1016/j.phytochem.2007.07.017, ISSN：0031-9422, PubMed ID：17822727, Web of Science ID：WOS:000253267300015
• Strigolactones, host recognition signals for root parasitic plants and arbuscular mycorrhizal fungi, from Fabaceae plants　　　　　　　　　　　　　　　
  Kaori Yoneyama; Xiaonan Xie; Hitoshi Sekimoto; Yasutomo Takeuchi; Shin Ogasawara; Kohki Akiyama; Hideo Hayashi; Koichi Yoneyama
  NEW PHYTOLOGIST, Volume：179, Number：2, First page：484, Last page：494, 2008, [Reviewed]
  Both root parasitic plants and arbuscular mycorrhizal (AM) fungi take advantage of strigolactones, released from plant roots as signal molecules in the initial communication with host plants, in order to commence parasitism and mutualism, respectively.
  In this study, strigolactones in root exudates from 12 Fabaceae plants, including hydroponically grown white lupin (Lupinus albus), a nonhost of AM fungi, were characterized by comparing retention times of germination stimulants on reverse-phase high-performance liquid chromatography (HPLC) with those of standards and by using tandem mass spectrometry (LC/MS/MS).
  All the plant species examined were found to exude known strigolactones, such as orobanchol, orobanchyl acetate, and 5-deoxystrigol, suggesting that these strigolactones are widely distributed in the Fabaceae. It should be noted that even the nonmycotrophic L. albus exuded orobanchol, orobanchyl acetate, 5-deoxystrigol, and novel germination stimulants.
  By contrast to the mycotrophic Fabaceae plant Trifolium pratense, in which phosphorus deficiency promoted strigolactone exudation, neither phosphorus nor nitrogen deficiency increased exudation of these strigolactones in L. albus. Therefore, the regulation of strigolactone production and/or exudation seems to be closely related to the nutrient acquisition strategy of the plants.
  WILEY-BLACKWELL, English, Scientific journal
  DOI：https://doi.org/10.1111/j.1469-8137.2008.02462.x
  DOI ID：10.1111/j.1469-8137.2008.02462.x, ISSN：0028-646X, PubMed ID：19086293, Web of Science ID：WOS:000257570400021
• Nitrogen deficiency as well as phosphorus deficiency in sorghum promotes the production and exudation of 5-deoxystrigol, the host recognition signal for arbuscular mycorrhizal fungi and root parasites　　　　　　　　　　　　　　　
  Kaori Yoneyama; Xiaonan Xie; Dai Kusumoto; Hitoshi Sekimoto; Yukihiro Sugimoto; Yasutomo Takeuchi; Koichi Yoneyama
  PLANTA, Volume：227, Number：1, First page：125, Last page：132, Dec. 2007, [Reviewed]
  Strigolactones released from plant roots induce hyphal branching of symbiotic arbuscular mycorrhizal (AM) fungi and germination of root parasitic weeds, Striga and Orobanche spp. We already demonstrated that, in red clover plants (Trifolium pratense L.), a host for both AM fungi and the root holoparasitic plant Orobanche minor Sm., reduced supply of phosphorus (P) but not of other elements examined (N, K, Ca, Mg) in the culture medium significantly promoted the secretion of a strigolactone, orobanchol, by the roots of this plant. Here we show that in the. case of sorghum [Sorghum bicolor (L.) Moench], a host of both the root hemiparasitic plant Striga hermonthica and AM fungi, N deficiency as well as P deficiency markedly enhanced the secretion of a strigolactone, 5-deoxystrigol. The 5-deoxystrigol content in sorghum root tissues also increased under both N deficiency and P deficiency, comparable to the increase in the root exudates. These results suggest that strigolactones may be rapidly released after their production in the roots. Unlike the situation in the roots, neither N nor P deficiency affected the low content of 5-deoxystrigol in sorghum shoot tissues.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-007-0600-5
  DOI ID：10.1007/s00425-007-0600-5, ISSN：0032-0935, PubMed ID：17684758, Web of Science ID：WOS:000251370300012
• 2 '-Epi-orobanchol and solanacol, two unique strigolactones, germination stimulants for root parasitic weeds, produced by tobacco　　　　　　　　　　　　　　　
  Xiaonan Xie; Dai Kusumoto; Yasutomo Takeuchi; Kaori Yoneyama; Yoichi Yamada; Koichi Yoneyama
  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Volume：55, Number：20, First page：8067, Last page：8072, Oct. 2007, [Reviewed]
  Germination stimulants for root holoparasitic weeds broomrapes (Orobanche and Phelipanche spp.) produced by tobacco (Nicotiana tabacum L.) were purified and characterized. The root exudates of tobacco contained at least five different stimulants, and LC-MS/MS analyses revealed that four of them were strigolactones; a tetraclehydrostrigol isomer, a didehydrostrigol isomer, and two strigol isomers. The two isomers of strigol were identified as (+)-orobanchol and its 2'-epimer by comparison of NMR and GC- and LC-MS data with those of synthetic standards. The structure of the tetradehydrostrigol isomer, the major stimulant of the bright yellow tobacco cultivars, was determined as 4-(x-hydroxy-5,8-dimethyl-GR24 [(E)-4-(x-hydroxy-5,8-dimethyl-3-(4-methyl-5-oxo-2,5-dihydrofuran2-yloxy)methylene)-3a,4-dihydro-3H-indeno[1,2-b]furan-2(8bH)-one] and named solanacol. 2'-Epiorobanchol and solanacol are the first natural strigolactones having a 2'-epi stereochemistry and a benzene ring, respectively.
  AMER CHEMICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1021/jf0715121
  DOI ID：10.1021/jf0715121, ISSN：0021-8561, PubMed ID：17803261, Web of Science ID：WOS:000249871100012
• Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not by jasmonate　　　　　　　　　　　　　　　
  Dai Kusumoto; Yaakov Goldwasser; Xiaonan Xie; Kaori Yoneyama; Yasutomo Takeuchi; Koichi Yoneyama
  ANNALS OF BOTANY, Volume：100, Number：3, First page：537, Last page：544, Sep. 2007, [Reviewed]
  Background and Aims Obligate root holoparasites of the genus Orobanche attack dicotyledonous crops and cause severe losses in many parts of the world. Chemical induction of plant defence systems such as systemic acquired resistance was proposed to be an available strategy to control the root parasite, but the detailed mechanisms involved have not been clarified. The aim of this study was to elucidate the effects of salicylic acid (SA), jasmonic acid (JA) and their analogues on resistance of red clover to Orobanche parasitism.
  Methods Roots of red clover grown in plastic chambers were applied with SA, S-methyl benzo[1,2,3]thiadiazole-7-carbothioate (BTH), methyl jasmonate (MeJA) and n-propyl dihydrojasmonate (PDJ), and then were inoculated with O. minor seeds. Attachments of the parasite were observed after 5 weeks.
  Key Results SA and BTH, inducers of SA-mediated defences, significantly reduced the number of established parasites by more than 75%. By contrast, MeJA and PDJ, inducers of JA-mediated defences, did not affect parasitism. The reduction in the number of established parasites by SA and BTH was due to the inhibited elongation of O. minor radicles and the activation of defence responses in the host root including lignification of the endodermis.
  Conclusions These results suggest that SA-induced resistance, but not JA-induced resistance, is effective in inhibiting Orobanche parasitism and that the resistance is expressed by the host root both externally and internally.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/aob/mcm148
  DOI ID：10.1093/aob/mcm148, ISSN：0305-7364, PubMed ID：17660517, Web of Science ID：WOS:000249654600010
• Phosphorus deficiency in red clover promotes exudation of orobanchol, the signal for mycorrhizal symbionts and germination stimulant for root parasites　　　　　　　　　　　　　　　
  Kaori Yoneyama; Koichi Yoneyama; Yasutomo Takeuchi; Hitoshi Sekimoto
  PLANTA, Volume：225, Number：4, First page：1031, Last page：1038, Mar. 2007, [Reviewed]
  Plant derived sesquiterpene strigolactones, which have previously been characterized as germination stimulants for root parasitic plants, have recently been identified as the branching factors which induce hyphal branching morphogenesis, a critical step in host recognition by arbuscular mycorrhizal (AM) fungi. We show here that, in red clover plants (Trifolium pratense L.), which is known as a host for both AM fungi and the root holoparasitic plant Orobanche minor Sm., reduced supply of phosphorus (P) but not of other elements examined (N, K, Mg, Ca) in the culture medium significantly promotes the release of a strigolactone, orobanchol, by the roots of this plant. In red clover plants, the level of orobanchol exudation appeared to be regulated by P availability and was in good agreement with germination stimulation activity of the root exudates. This implies that under P deficiency, plant roots attract not only symbiotic fungi but also root parasitic plants through the release of strigolactones. This is the first report demonstrating that nutrient availability influences both symbiotic and parasitic interactions in the rhizosphere.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-006-0410-1
  DOI ID：10.1007/s00425-006-0410-1, ISSN：0032-0935, PubMed ID：17260144, Web of Science ID：WOS:000244303900020
• Effects of fluridone and norflurazon on conditioning and germination of Striga asiatica seeds
  Dai Kusumoto; Sang Heon Chae; Ken Mukaida; Kaori Yoneyama; Koichi Yoneyama; Daniel M. Joel; Yasutomo Takeuchi
  Plant Growth Regulation, Volume：48, Number：1, First page：73, Last page：78, Jan. 2006, [Reviewed]
  Inhibitors of carotenoid biosynthesis are known to prevent abscisic acid (ABA) biosynthesis and to affect germination and dormancy of seeds in many plants. In this study, the effects of three carotenoid biosynthesis inhibitors, fluridone, norflurazon and diflufenican, on the conditioning and germination of Striga asiatica seeds were examined. Fluridone and norflurazon shortened the conditioning period required before S. asiatica seeds would germinate after exposure to the germination stimulant strigol, and prevented the inhibitory effects of both light and supraoptimal temperature (40°C) on seed germination. In addition, treatment with fluridone or norflurazon after conditioning in water induced seed germination in a manner similar to the effect of natural germination stimulants. Moreover, the seedlings developing after conditioned with fluridone formed haustorium-like structures without the involvement of haustorium inducing factors. In contrast, diflufenican had no effect on the conditioning and germination of S. asiatica seeds. These results indicate that fluridone and norflurazon have various effects on the germination of S. asiatica seeds and might be available for control of root parasites. © Springer 2006.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10725-005-4746-5
  DOI ID：10.1007/s10725-005-4746-5, ISSN：0167-6903, SCOPUS ID：30644467118

• 植物-植物間コミュニケーションを利用した作物の耐性強化を目指して　　　　　　　　　　　　　　　
  米山香織
  Volume：58, Number：10, 2025
  ISSN：0289-8233, J-Global ID：202502277505470930
• An Ancestral Strigolactone Conserved in Land Plants　　　　　　　　　　　　　　　
  YODA Akiyoshi; YODA Akiyoshi; XIE Xiaonan; SHIMAZAKI Shota; KODAMA Kyoichi; YONEYAMA Kaori; AKIYAMA Kohki; SHIMAMURA Masaki; KYOZUKA Junko; NOMURA Takahito
  日本植物生理学会年会(Web), Volume：62nd, 2021
  J-Global ID：202102273274181273
• Strigolactone Biosynthesis in Bryophytes　　　　　　　　　　　　　　　
  依田彬義; 依田彬義; XIE Xiaonan; 島崎翔太; 児玉恭一; 米山香織; 秋山康紀; 嶋村正樹; 経塚淳子; 野村崇人
  日本農芸化学会大会講演要旨集(Web), Volume：2021, 2021
  ISSN：2186-7976, J-Global ID：202102211657721084
• Functional characterization of sorghum LGS1, a target for controlling root parasitic weeds　　　　　　　　　　　　　　　
  野村崇人; 依田彬義; 森愛美; 三浦謙治; 米山香織; XIE Xiaonan; 秋山康紀; 米山弘一; 米山弘一
  日本農薬学会大会講演要旨集, Volume：45th, 2020
  ISSN：1347-8524, J-Global ID：202002281249229735
• ミヤコグサの5DSおよびlotuslactone生合成におけるMAX1下流経路の解析　　　　　　　　　　　　　　　
  森愛美; XIE Xiaonan; 米山香織; 野村崇人; 米山弘一; 秋山康紀
  Volume：2019, 2019
  ISSN：2186-7976, J-Global ID：201902218376039361
• ストリゴラクトン生合成酵素エンドウMAX1の機能解析　　　　　　　　　　　　　　　
  阿部友美; 秋山康紀; 依田彬義; 野村崇人; RAMEA Catherine; 米山香織; 米山香織
  Volume：54, Number：Supplement, 2019
  ISSN：1346-5406, J-Global ID：201902232408369605
• 立体選択的なストリゴラクトン生産に関与するソルガムLGS1酵素の機能解析　　　　　　　　　　　　　　　
  依田彬義; 森愛美; 齋藤睦美; 菊地麻祐; 神林瑛瑠; XIE Xiaonan; XIE Xiaonan; 米山香織; 秋山康紀; 米山弘一; 野村崇人; 野村崇人
  Volume：2019, 2019
  ISSN：2186-7976, J-Global ID：201902243622466315
• シダ植物のストリゴラクトン　　　　　　　　　　　　　　　
  藤倉優; 依田彬義; XIE Xiaonan; 米山香織; 秋山康紀; 経塚淳子; 米山弘一; 野村崇人
  Volume：54, Number：Supplement, 2019
  ISSN：1346-5406, J-Global ID：201902261080363353
• ストリゴラクトン生合成酵素LBOの機能解析
  米山香織; 米山香織; 秋山康紀; 高島岬; 依田彬義; XIE Xionan; 米山弘一; 野村崇人
  植物の生長調節, Volume：53, Number：Supplement, First page：20, 05 Oct. 2018
  Japanese
  ISSN：1346-5406, J-Global ID：201802278640451548
• ストリゴラクトン生合成のフィードバック制御
  米山香織; 米山香織; XIE Xionan; 米山弘一; 野村崇人
  植物の生長調節, Volume：53, Number：Supplement, First page：21, 05 Oct. 2018
  Japanese
  ISSN：1346-5406, J-Global ID：201802291810666229
• ポプラの根浸出物に含まれるストリゴラクトンの同定および構造解析　　　　　　　　　　　　　　　
  下野叡; 松下明真; 米山香織; 岡本昌憲; 野村崇人; 野村崇人; 米山弘一; XIE Xiaonan; XIE Xiaonan
  Volume：2018, 2018
  ISSN：2186-7976, J-Global ID：201802220094749975
• トマトのソラナコール生合成における芳香環形成経路　　　　　　　　　　　　　　　
  野上香奈; 米山香織; 野村崇人; 米山弘一; 秋山康紀
  Volume：2018, 2018
  ISSN：2186-7976, J-Global ID：201802262524762144
• Evolution of MAX1 enzymes in strigolactone biosynthesis　　　　　　　　　　　　　　　
  NOMURA Takahito; YONEYAMA Kaori; YONEYAMA Kaori; SATO Tomoyasu; YODA Akiyoshi; XIE Xiaonan; MORI Narumi; AKIYAMA Kohki; OKADA Kazunori; YOKOTA Takao; YONEYAMA Koichi
  日本植物生理学会年会(Web), Volume：59th, 2018
  J-Global ID：201802219073222414
• Hydroxycarlactone derivatives are potential substrates for MAX1 and LBO in strigolactone biosynthesis
  YONEYAMA Kaori; AKIYAMA Kohki; MORI Manami; XIE Xiaonan; YAMAUCHI Satoshi; NISHIWAKI Hisashi; YONEYAMA Koichi; NOMURA Takahito
  日本植物生理学会年会(Web), Volume：59th, First page：ROMBUNNO.1aG03, 2018
  English
  J-Global ID：201802251438337592
• シロイヌナズナにおける内生ストリゴラクトンの同定
  米山香織; 米山香織; 秋山康紀; 森愛美; XIE Xiaonan; 米山弘一; 野村崇人
  植物の生長調節, Volume：52, Number：Supplement, First page：45, 06 Oct. 2017
  Japanese
  ISSN：1346-5406, J-Global ID：201702273193413157
• ミヤコグサ5DS生合成におけるMAX1下流経路の解析　　　　　　　　　　　　　　　
  森愛美; 米山香織; XIE Xiaonan; 野村崇人; 米山弘一; 秋山康紀
  Volume：52, Number：Supplement, 2017
  ISSN：1346-5406, J-Global ID：201702218474729746
• ミヤコグサのストリゴラクトン生合成における新規MAX1産物の同定　　　　　　　　　　　　　　　
  森愛美; 米山香織; XIE Xiaonan; 野村崇人; 米山弘一; 秋山康紀
  Volume：2017, 2017
  ISSN：2186-7976, J-Global ID：201702220332141653
• ソラナコール型カーラクトン類のトマトMAX1およびLBOによる変換　　　　　　　　　　　　　　　
  野上香奈; 米山香織; 野村崇人; 米山弘一; 秋山康紀
  Volume：2017, 2017
  ISSN：2186-7976, J-Global ID：201702229266440653
• トマトが生産する新奇ストリゴラクトンの探索　　　　　　　　　　　　　　　
  XIE Xiaonan; 米山香織; 内田健一; 横田孝雄; 野村崇人; 米山弘一
  Volume：42nd, 2017
  ISSN：1347-8524, J-Global ID：201702233541957159
• イヌカタヒバMAX1の酵素機能　　　　　　　　　　　　　　　
  佐藤智康; 米山香織; 森愛美; XIE Xiaonan; XIE Xiaonan; 秋山康紀; 米山弘一; 米山弘一; 野村崇人; 野村崇人
  Volume：52, Number：Supplement, 2017
  ISSN：1346-5406, J-Global ID：201702253823907362
• MAX1とLBOにより導かれるストリゴラクトン生合成経路　　　　　　　　　　　　　　　
  野村崇人; 米山香織; 森愛実; XIE Xiaonan; 秋山康紀; 米山弘一
  Volume：2017, 2017
  ISSN：2186-7976, J-Global ID：201702257619487155
• ストリゴラクトン生合成におけるMAX1ホモログの機能解析　　　　　　　　　　　　　　　
  佐藤智康; 米山香織; 森愛美; 齋藤睦美; XIE Xiaonan; XIE Xiaonan; 秋山康紀; 米山弘一; 米山弘一; 野村崇人; 野村崇人
  Volume：2017, 2017
  ISSN：2186-7976, J-Global ID：201702282628919054
• シロイヌナズナにおける新奇ストリゴラクトン生合成酵素LBOの機能解析
  米山香織; BREWER Philip; 秋山康紀; 依田彬義; XIE Xiaonan; 瀬戸義哉; 山口信次郎; BEVERIDGE Christine; 米山弘一; 野村崇人
  植物の生長調節, Volume：51, Number：Supplement, First page：84, 07 Oct. 2016
  Japanese
  ISSN：1346-5406, J-Global ID：201602262247330004
• ストリゴラクトン生合成酵素MAX1の機能多様性
  米山香織; 森愛実; 秋山康紀; 佐藤智康; 齋藤睦美; XIE Xiaonan; 米山弘一; 野村崇人
  植物の生長調節, Volume：51, Number：Supplement, First page：83, 07 Oct. 2016
  Japanese
  ISSN：1346-5406, J-Global ID：201602283419267815
• Functional diversity of MAX1 enzymes in strigolactone biosynthesis
  YONEYAMA Kaori; MORI Narumi; XIE Xiaonan; KISUGI Takaya; OHNISHI Toshiyuki; LI Weiqiang; YOSHIDA Satoko; SHIRASU Ken; YAMAGUCHI Shinjiro; AKIYAMA Kohki; YONEYAMA Koichi; NOMURA Takahito
  日本植物生理学会年会要旨集, Volume：57th, First page：173, 11 Mar. 2016
  English
  J-Global ID：201602268026867923
• イネのストリゴラクトン生産におけるリンとサイトカイニンの影響
  米山香織; 米山弘一; 野村崇人
  日本農芸化学会大会講演要旨集(Web), Volume：2016, First page：2H016 (WEB ONLY), 05 Mar. 2016
  Japanese
  ISSN：2186-7976, J-Global ID：201602283053208296
• A future plan for further advancement of the Japanese society for chemical regulation of plants　　　　　　　　　　　　　　　
  Matsubayashi Yoshikatsu; Hayashi Ken-ichiro; Okamoto Masanori; Yamaguchi Shinjiro; Yoneyama Kaori
  植物化学調節学会研究発表記録集, Volume：50, Number：0, First page：16, Last page：16, 01 Oct. 2015
  The Japanese Society for Chemical Regulation of Plants, Japanese
  ISSN：0919-1887, CiNii Articles ID：110010014914, CiNii Books ID：AN10348274
• P039 Enzymatic function of Striga MAX1　　　　　　　　　　　　　　　
  Yoneyama Kaori; Mori Narumi; Li Weiqiang; Xie Xiaonan; Seto Yoshiya; Yoshida Satoko; Shirasu Ken; Akiyama Kohki; Yamaguchi Shinjiro; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：50, Number：0, First page：57, Last page：57, 01 Oct. 2015
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.50.Supplement_57
  DOI ID：10.18978/jscrpanb.50.Supplement_57, ISSN：0919-1887, CiNii Articles ID：110010014955
• Strigolactones are transported from roots to shoots, although not through the xylem　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Takaya Kisugi; Takahito Nomura; Kohki Akiyama; Tadao Asami; Koichi Yoneyama
  JOURNAL OF PESTICIDE SCIENCE, Volume：40, Number：3-4, First page：214, Last page：216, 2015
  Strigolactones (SLs) mainly produced in roots move upward to shoots and inhibit axillary bud outgrowth. However, SLs were not detected by LC-MS/MS in xylem saps collected from rice, tomato, cucumber, tobacco, sorghum, and Arabidopsis. d(1)-Orobanchol and d(6)-4-deoxyorobanchol that were fed to roots of rice plants were detected in shoots harvested 20 hr after treatment, although not in the xylem sap. These results imply that both endogenous and exogenous SLs are transported from roots to shoots, although not through the xylem. (c) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.D15-045
  DOI ID：10.1584/jpestics.D15-045, ISSN：1348-589X, eISSN：1349-0923, CiNii Articles ID：130005111001, Web of Science ID：WOS:000367027300022
• Strigolactones are transported from roots to shoots, although not through the xylem　　　　　　　　　　　　　　　
  Xiaonan Xie; Kaori Yoneyama; Takaya Kisugi; Takahito Nomura; Kohki Akiyama; Tadao Asami; Koichi Yoneyama
  JOURNAL OF PESTICIDE SCIENCE, Volume：40, Number：3-4, First page：214, Last page：216, 2015
  Strigolactones (SLs) mainly produced in roots move upward to shoots and inhibit axillary bud outgrowth. However, SLs were not detected by LC-MS/MS in xylem saps collected from rice, tomato, cucumber, tobacco, sorghum, and Arabidopsis. d(1)-Orobanchol and d(6)-4-deoxyorobanchol that were fed to roots of rice plants were detected in shoots harvested 20 hr after treatment, although not in the xylem sap. These results imply that both endogenous and exogenous SLs are transported from roots to shoots, although not through the xylem. (c) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.D15-045
  DOI ID：10.1584/jpestics.D15-045, ISSN：1348-589X, eISSN：1349-0923, J-Global ID：201502206727808547, CiNii Articles ID：130005105050, Web of Science ID：WOS:000367027300022
• 68. Enzymatic function of MAX1 in strigolactone biosynthesis　　　　　　　　　　　　　　　
  Abe Satoko; Sado Aika; Kisugi Takaya; Kim Hyun Il; Yoneyama Kaori; Xie Xiaonan; Ohnishi Toshiyuki; Akiyama Kohki; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：49, Number：0, First page：86, Last page：86, 01 Oct. 2014
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.49.Supplement_86
  DOI ID：10.18978/jscrpanb.49.Supplement_86, ISSN：0919-1887, CiNii Articles ID：110009865157
• 72. Shoot branching regulated by nitrogen nutrient and strigolactones in Arabidopsis　　　　　　　　　　　　　　　
  Sugiura Natsuki; Yoneyama Kaori; Xie Xiaonan; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：49, Number：0, First page：90, Last page：90, 01 Oct. 2014
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.49.Supplement_90
  DOI ID：10.18978/jscrpanb.49.Supplement_90, ISSN：0919-1887, CiNii Articles ID：110009865161
• 7 alpha- and 7 beta-Hydroxyorobanchyl acetate as germination stimulants for root parasitic weeds produced by cucumber　　　　　　　　　　　　　　　
  Pichit Khetkam; Xiaonan Xie; Takaya Kisugi; Hyun Il Kim; Kaori Yoneyama; Kenichi Uchida; Takao Yokota; Takahito Nomura; Koichi Yoneyama
  JOURNAL OF PESTICIDE SCIENCE, Volume：39, Number：3-4, First page：121, Last page：126, 2014
  Cucumber (Cucumis sativus) plants were found to exude at least 12 germination stimulants for root parasitic weeds including 5 known strigolactones, 7-oxoorobanchol, 7-oxoorobanchyl acetate, orobanchol, orobanchyl acetate, and 4-deoxyorobanchol. Two novel germination stimulants were purified from cucumber root exudates and their structures were determined to be 7 alpha- and 7 beta-hydroxyorobanchyl acetate by ID and 2D NMR spectroscopy, and ESI- and El-MS spectrometry. The stereochemistry was determined by NOE measurement and by comparing the CD spectra with those of the synthetic standards of four stereoisomers of orobanchol. 7 alpha- and 7 beta-Hydroxyorobanchol were detected by LC-MS/MS, and GC-MS analysis of purified sample confirmed their structures. The germination stimulation activities of 7 alpha- and 7 beta-hydroxyorobanchyl acetate on Orobanche minor were comparable to those of orobanchyl acetate and 7-oxoorobanchyl acetate. By contrast, 7 beta-hydroxyorobanchyl acetate was a highly potent germination stimulant for Phelipanche ramosa inducing more than 50% germination at 10 pM. (C) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.D14-038
  DOI ID：10.1584/jpestics.D14-038, ISSN：1348-589X, eISSN：1349-0923, J-Global ID：201402279445760044, Web of Science ID：WOS:000346892300002
• 7 alpha- and 7 beta-Hydroxyorobanchyl acetate as germination stimulants for root parasitic weeds produced by cucumber　　　　　　　　　　　　　　　
  Pichit Khetkam; Xiaonan Xie; Takaya Kisugi; Hyun Il Kim; Kaori Yoneyama; Kenichi Uchida; Takao Yokota; Takahito Nomura; Koichi Yoneyama
  JOURNAL OF PESTICIDE SCIENCE, Volume：39, Number：3-4, First page：121, Last page：126, 2014
  Cucumber (Cucumis sativus) plants were found to exude at least 12 germination stimulants for root parasitic weeds including 5 known strigolactones, 7-oxoorobanchol, 7-oxoorobanchyl acetate, orobanchol, orobanchyl acetate, and 4-deoxyorobanchol. Two novel germination stimulants were purified from cucumber root exudates and their structures were determined to be 7 alpha- and 7 beta-hydroxyorobanchyl acetate by ID and 2D NMR spectroscopy, and ESI- and El-MS spectrometry. The stereochemistry was determined by NOE measurement and by comparing the CD spectra with those of the synthetic standards of four stereoisomers of orobanchol. 7 alpha- and 7 beta-Hydroxyorobanchol were detected by LC-MS/MS, and GC-MS analysis of purified sample confirmed their structures. The germination stimulation activities of 7 alpha- and 7 beta-hydroxyorobanchyl acetate on Orobanche minor were comparable to those of orobanchyl acetate and 7-oxoorobanchyl acetate. By contrast, 7 beta-hydroxyorobanchyl acetate was a highly potent germination stimulant for Phelipanche ramosa inducing more than 50% germination at 10 pM. (C) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.D14-038
  DOI ID：10.1584/jpestics.D14-038, ISSN：1348-589X, eISSN：1349-0923, CiNii Articles ID：130004445312, Web of Science ID：WOS:000346892300002
• 43. Identification of 7β-hydroxy-5-deoxystrigol from root exudates of dokudami　　　　　　　　　　　　　　　
  Kisugi Takaya; Nakatani Yoshifumi; Xie Xiaonan; Yoneyama Kaori; Akiyama Kohki; Hayashi Hideo; Uchida Kenichi; Yokota Takao; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：48, Number：0, First page：58, Last page：58, 04 Oct. 2013
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.48.Supplement_58
  DOI ID：10.18978/jscrpanb.48.Supplement_58, ISSN：0919-1887, CiNii Articles ID：110009738252
• 44. Analysis of endogenous strigolactones in Striga hermonthica　　　　　　　　　　　　　　　
  Abe Satoko; Xie Xiaonan; Kisugi Takaya; Yoneyama Kaori; Yoshida Satoko; Shirasu Ken; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：48, Number：0, First page：59, Last page：59, 04 Oct. 2013
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.48.Supplement_59
  DOI ID：10.18978/jscrpanb.48.Supplement_59, ISSN：0919-1887, CiNii Articles ID：110009738253
• 62. Sucrose restores strigolactone production in shoot removed rice plant　　　　　　　　　　　　　　　
  Yoneyama Kaori; Kisugi Takaya; Xie Xiaonan; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：48, Number：0, First page：77, Last page：77, 04 Oct. 2013
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.48.Supplement_77
  DOI ID：10.18978/jscrpanb.48.Supplement_77, ISSN：0919-1887, CiNii Articles ID：110009738271
• 63. Strigolactone biosynthesis is regulated by nitrogen in Arabidopsis　　　　　　　　　　　　　　　
  Sugiura Natsuki; Yoneyama Kaori; Kisugi Takaya; Xie Xiaonan; Yamaguchi Shinjiro; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：48, Number：0, First page：78, Last page：78, 04 Oct. 2013
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.48.Supplement_78
  DOI ID：10.18978/jscrpanb.48.Supplement_78, ISSN：0919-1887, CiNii Articles ID：110009738272
• P4-1-13 菌根菌に対する受容性が異なるトウモロコシ品種における菌根/リン応答性とストリゴラクトン分泌特性(4-1 植物の多量栄養素)　　　　　　　　　　　　　　　
  荒川 竜太; 米山 香織; 小林 創平; 米山 弘一; 江沢 辰広
  Number：59, First page：65, Last page：65, 11 Sep. 2013
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110009813320, CiNii Books ID：AN00195778
• Studies on effects of plant nutrients on strigolactone production and exudation(The JSCRP Award for the Encouragement of Young Scientist,The Society Awards Lecture)　　　　　　　　　　　　　　　
  Yoneyama Kaori
  Regulation of Plant Growth & Development, Volume：48, Number：1, First page：24, Last page：32, 31 May 2013
  Availability of mineral nutrients affects strigolactone (SL) production and exudation from plants. In legumes which form symbiotic relationship with root nodule bacteria, deficiency of phosphorus (P) but not other nutrients including nitrogen (N) significantly promoted SL production and exudation. By contrast, in non-legume plants, N deficiency as well as P deficiency increased SL exudation. Such a promotion of SL exudation under nutrient deficiency could be observed, in general, with mycotrophic plants but not with non-mycotrophic plants including Arabidopsis and white lupin.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrp.48.1_24
  DOI ID：10.18978/jscrp.48.1_24, ISSN：1346-5406, CiNii Articles ID：110009615965, CiNii Books ID：AA11550064
• 57. Isolation and characterization of strigolactones exuded by maiz　　　　　　　　　　　　　　　
  Khetkam Pichit; Kisugi Takaya; Xie Xiaonan; Yoneyama Kaori; Nomura Takahito; Yoneyama Koichi
  Volume：47, First page：74, Last page：74, 05 Oct. 2012
  The Japanese Society for Chemical Regulation of Plants, English
  ISSN：0919-1887, CiNii Articles ID：110009517283, CiNii Books ID：AN10348274
• 56. Characterization of strigolactones exuded from Orobanche foetida tolerant and susceptible faba bean genotypes　　　　　　　　　　　　　　　
  Trabelsi Imen; Yoneyama Kaori; Abbes Zouhaier; Xie Xiaonan; Amri Moez; Kharrat Mohamed; Yoneyama Koichi
  Volume：47, First page：73, Last page：73, 05 Oct. 2012
  The Japanese Society for Chemical Regulation of Plants, English
  ISSN：0919-1887, CiNii Articles ID：110009517282, CiNii Books ID：AN10348274
• Studies on effects of plant nutrients on strigolactone production and exudation　　　　　　　　　　　　　　　
  Yoneyama Kaori
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：17, Last page：17, 05 Oct. 2012
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110009517226, CiNii Books ID：AN10348274
• 45. Synthesis of all regio- and stereoisomers of 5-deoxystrigol derivatives substituted with a methoxy group in the AB ring　　　　　　　　　　　　　　　
  Sado Aika; Yamada Akihiro; Yoneyama Kaori; Xie Xiaonan; Yoneyama Koichi; Akiyama Kohki; Hayashi Hideo
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：62, Last page：62, 05 Oct. 2012
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110009517271, CiNii Books ID：AN10348274
• 50. Identifi cation of germination stimulants for root parasitic plants produced by Brassica species　　　　　　　　　　　　　　　
  Kyushima Akimi; Yoneyama Kaori; Kisugi Takaya; Xie Xiaonan; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：67, Last page：67, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_67
  DOI ID：10.18978/jscrpanb.47.Supplement_67, ISSN：0919-1887, CiNii Articles ID：110009517276
• 52. Germination stimulant strigolactones exuded by oilseed rape　　　　　　　　　　　　　　　
  Arai Riho; Yoneyama Kaori; Xie Xiaonan; Kisugi Takaya; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：69, Last page：69, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_69
  DOI ID：10.18978/jscrpanb.47.Supplement_69, ISSN：0919-1887, CiNii Articles ID：110009517278
• 53. Characterization of strigolactones produced by English ivy(Hedera helix)　　　　　　　　　　　　　　　
  Kim Hyunil; Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：70, Last page：70, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_70
  DOI ID：10.18978/jscrpanb.47.Supplement_70, ISSN：0919-1887, CiNii Articles ID：110009517279
• 54. Structural elucidation of novel strigolactone produced by dokudami　　　　　　　　　　　　　　　
  Kisugi Takaya; Xie Xiaonan; Kim Hyun Il; Yoneyama Kaori; Sado Aika; Akiyama Kohki; Hayashi Hideo; Uchida Kenichi; Yokota Takao; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：71, Last page：71, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_71
  DOI ID：10.18978/jscrpanb.47.Supplement_71, ISSN：0919-1887, CiNii Articles ID：110009517280
• 55. Characterization of strigolactones produced by tobacco　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Uchida Kenichi; Ito Seisuke; Akaiyama Kohki; Hayashi Hideo; Yokota Takao; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：72, Last page：72, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_72
  DOI ID：10.18978/jscrpanb.47.Supplement_72, ISSN：0919-1887, CiNii Articles ID：110009517281
• 58. Endogenous strigolactones in cultured cells of strigolactone-defi cient mutants　　　　　　　　　　　　　　　
  Yamanaka Koichi; Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Akiyama Koki; Yamaguchi Shinjiro; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：75, Last page：75, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_75
  DOI ID：10.18978/jscrpanb.47.Supplement_75, ISSN：0919-1887, CiNii Articles ID：110009517284
• 59. Effects of nutrient defi ciency on strigolactone exudation in pea rms mutants　　　　　　　　　　　　　　　
  Sasase Erina; Yoneyama Kaori; Ishii Yuuki; Foo Eloise; Hugill Cassandra; Quittenden Laura; Reid James B; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：76, Last page：76, 05 Oct. 2012
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110009517285, CiNii Books ID：AN10348274
• 60. Effect of nutrient defi ciency on strigolactone exudation in Orobanche foetida susceptible faba bean genotype　　　　　　　　　　　　　　　
  Ishii Yuuki; Yoneyama Kaori; Sasase Erina; Trabelsi Imen; Abbes Zouhaier; Amri Moez; Kharrat Mohamed; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：77, Last page：77, 05 Oct. 2012
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110009517286, CiNii Books ID：AN10348274
• 61. Interaction between phosphate and cytokinin in strigolactone production　　　　　　　　　　　　　　　
  Yoneyama Kaori; Xie Xiaonan; Kisugi Takaya; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：47, Number：0, First page：78, Last page：78, 05 Oct. 2012
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.47.Supplement_78
  DOI ID：10.18978/jscrpanb.47.Supplement_78, ISSN：0919-1887, CiNii Articles ID：110009517287
• アレロパシー植物セイヨウチャヒキが生産する発芽刺激物質の探索　　　　　　　　　　　　　　　
  高根沢恵子; 野村卓史; 米山香織; 来生貴也; 藤井義晴; 野村崇人; XIE Xiaonan; 米山弘一
  植物の生長調節, Volume：46, Number：Supplement, First page：64, 03 Oct. 2011
  Japanese
  ISSN：1346-5406, J-Global ID：201102245913447398
• 45. Characterization of strigolactones produced by Marchantia polymorpha L.　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Delaux Pierre-Marc; Becard Guillaume; Sejalon-Delmas Nathalie; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：61, Last page：61, 03 Oct. 2011
  At the previous meeting, we reported that Physcomitrella patens subsp. patens, a bryophyte which is a basal lineage of land plants, produces strigolactones (SLs). In the present study, strigolactones produced by Marchantia polymorpha L. which is evolutionary most apart from angiosperms, were examined. Marchantia was grown on 1/2 B5 solid media for 10, 15, 20, 25, 30, 35 and 40 days, and extracted with ethyl acetate, respectively. Characterization of SLs in these extracts was conducted by comparing retention times of germination stimulants on reversed phase HPLC with those of synthetic and/or natural standards and by using LC-MS/MS. LC-MS/MS analyses confirmed the presence of SLs in Marchantia extracts, and identified solanacol, two orobanchol isomers, 2'-epiorobanchyl acetate, fabacyl acetate and 5-deoxystrigol. These results indicate that strigolactones appeared when plants first colonized land.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_61
  DOI ID：10.18978/jscrpanb.46.0_61, ISSN：0919-1887, CiNii Articles ID：110008902454
• 46. Characterization of strigolactones produced by faba bean (Vicia faba L.)　　　　　　　　　　　　　　　
  Kyushima Akimi; Kisugi Takaya; Xie Xiaonan; Yoneyama Kaori; Uchida Kenichi; Fernandez-Aparicio Monica; Rubiales Diego; Yokota Takao; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：62, Last page：62, 03 Oct. 2011
  Broomrapes (Orobanche spp.) cause significant losses to agricultural production all over the world. The seeds of these parasites germinate only when they are exposed to the germination stimulants including strigolactones (SLs) produced by and released from host roots. In this study, characterization of strigolactones produced by faba bean (Vicia faba), a host of O. crenata and O. foetida was conducted. Faba bean was found to produce orobanchol and ent-2'-epiorobanchyl acetate by LC-MS/MS analysis of root exudates. Bioassay-guided purification to isolate novel germination stimulants is in progress.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_62
  DOI ID：10.18978/jscrpanb.46.0_62, ISSN：0919-1887, CiNii Articles ID：110008902455
• 47. Investigation of novel strigolactones produced by dokudami　　　　　　　　　　　　　　　
  Kisugi Takaya; Xie Xiaonan; Yoneyama Kaori; Uchida Kenichi; Yokota Takao; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：63, Last page：63, 03 Oct. 2011
  Previously, we reported that dokudami (Houttuynia cordata Thunb.) produce 5 known strigolactones (SLs), strigol, sorgomol, ent-2'-epiorobanchyl acetate, fabacyl acetate and 5-deoxystrigol, and novel SLs. HR-MS analyses of novel SLs revealed that their molecular are the same to that of strigol. One of them was suggested to be a positional isomer of strigol by 'H-NMR analysis. In addition to these SLs, presence of other novel SLs was indicated by bioassay-guided fractionation.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_63
  DOI ID：10.18978/jscrpanb.46.0_63, ISSN：0919-1887, CiNii Articles ID：110008902456
• 48. Characterization of germination stimulants produced by Avena strigosa, an allelopathic plant　　　　　　　　　　　　　　　
  Takanezawa Keiko; Nomura Takashi; Yoneyama Kaori; Kisugi Takaya; Fujii Yoshiharu; Nomura Takahito; Xie Xiaonan; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：64, Last page：64, 03 Oct. 2011
  Among the plant-derived germination stimulants for root parasitic plants, strigolactones (SLs) have been shown to be most widely distributed in the plant kingdom. SLs are branching factors of symbiotic arbuscular mycorrhizal fungi, and a novel class of plant hormones inhibiting shoot branching. All the plants investigated so far have been found to produce SLs and the total number of natural SLs have exceeded 30 including those remained to be characterized. In this study, characterization of germination stimulants produced by black oat (Avena strigosa), an allelopathic plant, was conducted.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_64
  DOI ID：10.18978/jscrpanb.46.0_64, ISSN：0919-1887, CiNii Articles ID：110008902457
• 49. Germination stimulant strigolactones produced by hairy vetch　　　　　　　　　　　　　　　
  Nomura Takashi; Xie Xiaonan; Yoneyama Kaori; Kim Hyun Il; Kisugi Takaya; Nomura Takahito; Fjii Yoshiharu; Yonenama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：65, Last page：65, 03 Oct. 2011
  Hairy vetch (Vicia villosa Roth.), which produces large amounts of cyanamid, is widely utilized as a winter cover crop in the United States and Japan. The use of trap crops which induce 'suicidal germination' is one of the control methods for root parasitic weeds. Among germination stimulants for root parasitic weeds, dihydrosorgoleone, sesquiterpene lactones, and strigolactones (SLs), SLs are known to be most widely distributed in land plants. In this study, identification of SLs produced by hairy vetch plants was conducted. LC-MS/MS (MRM) analysis revealed that hairy vetch produces fabacyl acetate, ent-2'-epiorobanchyl acetate and 5-deoxystrigol. Time-course of strigolactone production will also be presented.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_65
  DOI ID：10.18978/jscrpanb.46.0_65, ISSN：0919-1887, CiNii Articles ID：110008902458
• 50. Seed germination stimulants for Phelipanche ramosa produced by oilseed rape Brassica napus　　　　　　　　　　　　　　　
  Yoneyama Kaori; Xie Xiaonan; Kim Hyun Il; Kisugi Takaya; Nomura Takahito; Auger Bathilde; Delavault Philippe; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：66, Last page：66, 03 Oct. 2011
  Phelipanche ramosa, one of devastating root parasitic weeds, causes severe damages to oilseed rape (Brassica napus) in southern France. Since oilseed rape is a non-host for AM fungi, this plant seems to produce only small amounts of strigolactones (SLs) as germination stimulants. Brassica spp. produces glucosinolates which are enzymatically converted to afford isothiocyanates (ITCs), and ITCs induce germination of P. ramosa. In the present study, identification of SLs and ITCs produced by oilseed rape plants were conducted to clarify the main germination stimulants for P. ramosa.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_66
  DOI ID：10.18978/jscrpanb.46.0_66, ISSN：0919-1887, CiNii Articles ID：110008902459
• 51. Structure-activity relationship of strigolactones in root parasitic weed seed germination stimulation　　　　　　　　　　　　　　　
  Kim Hyun Il; Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Nomura Takahito; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：67, Last page：67, 03 Oct. 2011
  Strigolactones (SLs) work as germination stimulants of root parasitic plants and hyphal branching factors of symbiotic arbuscular mycorrhizal fungi. In addition to these functions of SLs in the rhizosphere, SLs or their further metabolites act as a class of plant hormones inhibiting shoot branching. In the present study, naturally occurring SLs, including those isolated recently in our laboratory, were examined for their germination stimulation on the seeds of a root parasitic plant, Orobanche minor Sm., and the structure-activity relationships of SLs in the stimulation of O. minor seed germination are discussed.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_67
  DOI ID：10.18978/jscrpanb.46.0_67, ISSN：0919-1887, CiNii Articles ID：110008902460
• 52. Studies on the biosynthesis pathway of strigolactones using plant cell cultures　　　　　　　　　　　　　　　
  Yamanaka Koichi; Xie Xiaonan; Yoneyama Kaori; Kisugi Takaya; Ueno Kotomi; Asami Tadao; Akiyama Koki; Yamaguchi Shinjiro; Yoneyama Koichi; Nomura Takahito
  植物化学調節学会研究発表記録集, Volume：46, Number：46, First page：68, Last page：68, 03 Oct. 2011
  Strigolactones (SLs) are host recognition signals for root parasitic plants and arbuscular mycorrhizal fungi in the rhizosphere. In addition, shoot branching is inhibited by SLs in host plants. It has been demonstrated that SLs are derived from the carotenoid pathway in plants. However, the biosynthesis pathway of SLs has not been fully elucidated by genetic approaches. In order to propose a possible biosynthesis pathway of SLs in plants we investigated SL metabolisms by a feeding experiment using plant cell cultures. We found that plant cultured cell lines of rice and Arabidopsis produce SLs at higher levels than in plants and examined an optimum condition for the SL feeding experiment. Feeding experiments were carried out using the Oc strain, a suspension cell culture of rice provided from RIKEN BioResource Center of Japan, with deuterium-labeled SLs. Deuterium-labeled 5-deoxystrigol and 2'-epi-5-deoxystrigol were converted into several monohydroxylated metabolites in rice Oc cells. These metabolites were not identical with known monohydroxylated SLs such as strigol, orobanchol and sorgomol, but some of them had a germination-stimulant activity in bioassay using Orobanche seeds.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.46.0_68
  DOI ID：10.18978/jscrpanb.46.0_68, ISSN：0919-1887, CiNii Articles ID：110008902461
• 34 トウモロコシにおけるストリゴラクトン類分泌プロファイルは共生・寄生生物の受容性と関係する(北海道支部講演会,2010年度各支部会講演要旨)　　　　　　　　　　　　　　　
  石本 圭子; 米山 香織; 小林 創平; 信濃 卓郎; 大崎 満; 横田 孝雄; 米山 弘一; 江沢 辰広
  Number：57, First page：297, Last page：297, 08 Aug. 2011
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110009439776, CiNii Books ID：AN00195778
• Abamine as a basis for new designs of regulators of strigolactone production　　　　　　　　　　　　　　　
  Nobutaka Kitahata; Shinsaku Ito; Atsutaka Kato; Kotomi Ueno; Takeshi Nakano; Kaori Yoneyama; Koichi Yoneyama; Tadao Asami
  JOURNAL OF PESTICIDE SCIENCE, Volume：36, Number：1, First page：53, Last page：57, 2011
  Nine-cis-epoxycarotenoid dioxygenase (NCED), belonging to the family of carotenoid-cleaving dioxygenases (CCDs), is the rate-limiting step in the abscisic acid (ABA) biosynthetic pathway in plants. Abamine is the first reported abscisic acid biosynthesis inhibitor to target NCED. Its utility has also been considered in the inhibition of other enzymes. As CCDs other than NCED are also involved in the strigolactone biosynthetic pathway, abamine may affect strigolactone biosynthesis. In this study, we found that abamine decreases the level of strigolactones in several plant species and reduces the germination rate of Orobanche minor seeds co-grown with tobacco. Taken together, these data suggest that abamine may be used as a scaffold for the development of specific regulators of strigolactone production. (C) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.G10-72
  DOI ID：10.1584/jpestics.G10-72, ISSN：1348-589X, CiNii Articles ID：120005321383, Web of Science ID：WOS:000289705500010
• Strigolactones as germination signals for root parasitic plants(Strigolactone)　　　　　　　　　　　　　　　
  Yoneyama Koichi; Xie Xiaonan; Yoneyama Kaori
  Regulation of Plant Growth & Development, Volume：45, Number：2, First page：83, Last page：94, 20 Dec. 2010
  Strigolactones (SLs) were originally isolated from plant root exudates as germination stimulants for root parasitic plants of the family Orobanchaceae, including witchweeds (Striga spp.), broomrapes (Orobanche and Phelipanche spp.), and Alectra spp., and so were regarded as detrimental to the producing plants. Most of these root parasites cause devastating damages to agricultural production in particular in developing countries, and their effective and economically feasible control measures have not yet been established. In this review, the unique host-dependent lifecycle of, discovery of SLs as germination stimulants for, and possible contribution of SLs to the host recognition of root parasitic plants are summarized.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrp.45.2_83
  DOI ID：10.18978/jscrp.45.2_83, ISSN：1346-5406, CiNii Articles ID：110008006476, CiNii Books ID：AA11550064
• 22. Feeding experiments of strigolactones using plant cell cultures　　　　　　　　　　　　　　　
  Nomura Takahito; Yamanaka Koichi; Xie Xiaonan; Kisugi Takaya; Yoneyama Kaori; Ueno Kotomi; Asami Tadao; Yokota Takao; Yamaguchi Shinjiro; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：45, Number：45, First page：39, Last page：39, 01 Oct. 2010
  Root parasitic plants and arbuscular mycorrhizal fungi receive strigolactones as host recognition signals in the rhizosphere. In host plants, strigolactones play a key role in shoot branching. Several lines of genetic evidence have illustrated that strigolantones are derived from the carotenoid pathway in plants. However, the biosynthesis pathway of strigolactones has not yet been fully elucidated. Establishment of their biosynthesis pathway and subsequent studies on regulation of their biosynthesis will provide important clues to control their physiological functions. To better understand the biosynthesis of strigolactones in plants, we investigated the occurrence of strigolactones and regulation of their biosynthesis in plant cultured cell lines provided from RIKEN BioResource Center of Japan. Feeding experiments using deuterium-labeled strigolactones in the plant cell cultures are now being conducted to unveil unestablished pathways in strigolactone biosynthesis.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.45.0_39
  DOI ID：10.18978/jscrpanb.45.0_39, ISSN：0919-1887, CiNii Articles ID：110007767122
• 23. Identification and structure determination of strigolactones from rice root exudate　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Uchida Kenichi; Yokoyama Mori; Kisugi Takaya; Yokota Takao; Nomura Takahito; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：45, Number：45, First page：40, Last page：40, 01 Oct. 2010
  Strigolactones function as germination stimulants of root parasitic plants and hyphal branching factors of symbiotic arbuscular mycorrhizal fungi in the rhizosphere, and in plants they act as a novel class of plant hormones inhibiting shoot branching. In this study, characterization of strigolactones in rice (Oryza sativa L.) root exudate was conducted. Rice root exudate was found to contain strigolactones including orobanchol, 2'-epiorobanchol, 7-oxoorobanchyl acetate, two 5-deoxystrigol isomers and some novel strigolactones. One of the 5-deoxystrigol isomer was identified as ent-2'-epi-5-deoxystrigol by spectroscopic analyses, and purification and structure elucidation of the other novel strigolactones will be presented.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.45.0_40
  DOI ID：10.18978/jscrpanb.45.0_40, ISSN：0919-1887, CiNii Articles ID：110007767123
• 24. Analysis of strigolactones exuded by Striga resistant and susceptible maize cultivars　　　　　　　　　　　　　　　
  Yoneyama Kaori; Ishimoto Keiko; Xie Xiaonan; Kisugi Takaya; Asahina Masashi; Nomura Takahito; Takeuchi Yasutomo; Yoneyama Koichi; Yokota Takao
  植物化学調節学会研究発表記録集, Volume：45, Number：45, First page：41, Last page：41, 01 Oct. 2010
  Striga spp., devastating root parasitic weeds, attack monocot crops including sorghum, millet and maize in semi-arid tropics. Their seeds require germination stimulants (mainly strigolactone, SL) released from host roots to germinate. In the present study, characterization of SLs in the root exudates from maize plants including the Striga susceptible cultivar Pioneer 3253 and tolerant cultivars KST94 and WH502, grown hydroponically was conducted by comparing retention times of germination stimulants on reversed phase HPLC with those of synthetic standards and by using LC/MS/MS. The susceptible cultivar mainly exuded larger amounts of 5-deoxystrigol and the tolerant cultivars exuded mainly strigol, sorgomol and orobanchol. 5-Deoxystrigol is more stable than hydroxy-SLs like strigol and thus susceptible cultivars might induce more germination of Striga seeds in the fields.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.45.0_41
  DOI ID：10.18978/jscrpanb.45.0_41, ISSN：0919-1887, CiNii Articles ID：110007767124
• 25. Characterization of strigolactones produced by gametophore of Physcomitrella patens and its ccd8 mutant　　　　　　　　　　　　　　　
  Sawabe Makiko; Xie Xiaonan; Yoneyama Kaori; Proust Helene; Hoffmann Beate; Rameau Catherine; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：45, First page：42, Last page：42, 01 Oct. 2010
  At the previous meeting, we reported that chloronema of Physcomitrella patens subsp. patens produces strigolactones (SLs). In this study, WT and the CCD8 loss-of-function mutant (Ppccd8) of P. patens were grown in liquid BCD culture for about 30 days to obtain gametophores during which the media were replaced with fresh one weekly. Then, they were grown for additional 3 days and media collected were extracted with ethyl acetate and SLs in the extracts were analyzed by LC-MS/MS. Both WT and Ppccd8 were found to produce 7-oxoorobanchyl acetate and strigol at similar levels, while fabacyl acetate, orobanchyl acetate, 7α-hydroxyorobanchyl acetate and orobanchol were produced only by WT. Although there is no MAX1 homolog in the moss genome, P. patens produces SLs, suggesting that MAX1 functions in later steps of the active branching inhibiting hormone biosynthesis, i.e, conversion of SLs to the branching inhibitor. Alternatively the moss may have other P450(s) which replace the function of MAX1 for SL biosynthesis.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007767125, CiNii Books ID：AN10348274
• 8-9 根寄生植物ストライガ耐性および感受性トウモロコシが分泌するストリゴラクトンへのアーバスキュラー菌根菌群の感染応答(8.共生,2010年度北海道大会)　　　　　　　　　　　　　　　
  石本 圭子; 米山 香織; 大崎 満; 横田 孝雄; 米山 弘一; 江沢 辰広
  Number：56, First page：46, Last page：46, 07 Sep. 2010
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110008510384, CiNii Books ID：AN00195778
• 2B04 Identification and structure determination of strigolactones from Cucumis sativus　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Uchida Kenichi; Nomura Takahito; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Number：35, First page：57, Last page：57, 21 Apr. 2010
  日本農薬学会, Japanese
  CiNii Articles ID：110009761682, CiNii Books ID：AN00387542
• 2B05 Cytokinins inhibit strigolactone production in rice　　　　　　　　　　　　　　　
  Yoneyama Kaori; Asahina Masashi; Xie Xiaonan; Nomura Takahito; Takeuchi Yasutomo; Yokota Takao; Yoneyama Koichi
  講演要旨集, Number：35, First page：58, Last page：58, 21 Apr. 2010
  日本農薬学会, Japanese
  CiNii Articles ID：110009761683, CiNii Books ID：AN00387542
• Structure-activity relationship of naturally occurring strigolactones in Orobanche minor seed germination stimulation　　　　　　　　　　　　　　　
  Hyun Il Kim; Xiaonan Xie; Han Sung Kim; Jae Chul Chun; Kaori Yoneyama; Takahito Nomura; Yasutomo Takeuchi; Koichi Yoneyama
  JOURNAL OF PESTICIDE SCIENCE, Volume：35, Number：3, First page：344, Last page：347, 2010
  Eleven naturally occurring strigolactones (SLs) were examined for their germination-stimulating activity on the seeds of a root parasitic plant Orobanche minor Sm. Based on their activity, SLs are classified into 3 groups (A-C). Group A, the most active germination stimulant, consists of 3 monohydroxy-SLs, orobanchol, 2'-epiorobanchol, and sorgomol, inducing >80% germination of O. minor seeds at 10 pM. Group B includes 5 SLs, which were ca. 10-fold less active than those in group A. The 3 least active SLs in group C were either more lipophilic or probably less stable than SLs in groups A and B. These results indicate that the germination-stimulating activity of SLs depends on the lipophilicity of the SL molecules and their stability also influences activity. Other structural features for germination-stimulating activity are also discussed. (C) Pesticide Science Society of Japan
  PESTICIDE SCI SOC JAPAN, English
  DOI：https://doi.org/10.1584/jpestics.G10-17
  DOI ID：10.1584/jpestics.G10-17, ISSN：1348-589X, CiNii Articles ID：120005320287, Web of Science ID：WOS:000282860900017
• 55. Effects of GA, IAA, ABA, brassinosteroids and progesterone on antheridium formation and protonemal elongation in Japanese climbing fern　　　　　　　　　　　　　　　
  Yoneyama Kaori; Shibata Kyomi; Yokota Takao
  植物化学調節学会研究発表記録集, Number：44, First page：69, Last page：69, 06 Oct. 2009
  Antheridium of L. japonicum is known to be induced by GA methyl ester. We earlier found that progesterone strongly inhibits GA4-Me induced antheridium formation. This paper reports that IAA and ABA strongly inhibited antheridium formation of L. japonicum. IAA was more biologically active than ABA and progesterorone, 3 times and 14 times, respectively. Protonemal elongation was enhanced by IAA but inhibited by ABA. No biological activity of brassinosteroids, brassinolide and castasterone, was observed in this biological system.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468315, CiNii Books ID：AN10348274
• 56. Effects of auxin and cytokinin on strigolactone production and exudation　　　　　　　　　　　　　　　
  Yoneyama Kaori; Asahina Masashi; Nomura Takahito; Yokota Takao; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：44, Number：44, First page：70, Last page：70, 06 Oct. 2009
  Strigolactone released from plant root stimulates germination of root parasitic plants and induces hyphal branching of arbuscular mycorrhizal fungi in the rhizosphere. In plant, strigolactone works as a plant hormone inhibiting shoot branching. It was already reported that phosphorus (P) deficiency promoted but P fertilization suppressed strigolactone exudation. In the present study, we demonstrated that auxin increased strigolactone exudation when rice plants were subjected to P fertilization, while cytokinin decreased strigolactone exudation when rice plants faced to P deficiency.
  植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrpanb.44.0_70
  DOI ID：10.18978/jscrpanb.44.0_70, ISSN：0919-1887, CiNii Articles ID：110007468316
• 63. Search for strigolactone biosynthesis inhibitors　　　　　　　　　　　　　　　
  Kato Atsutaka; Kitahata Nobutaka; Ito Shinsaku; Ueno Kotomi; Yoneyama Kaori; Suzuki Yoshihito; Yoneyama Koichi; Kyozuka Junko; Nakano Takeshi; Asami Tadao
  植物化学調節学会研究発表記録集, Number：44, First page：77, Last page：77, 06 Oct. 2009
  Strigolactones are plant secondary metabolites that function as host recognition signals for arbuscular mycorrhizal fungi and root parasitic plants, Striga and Orobanche. Recently, it has been reported that strigolactones act as a novel hormone in shoot branching. In this study, we try to design a strigolactone biosynthesis inhibitor. Strigolactones are synthesized from carotenoids, and in a proposed strigolactione biosynthesis pathway, carotenoid cleavage dioxygenase 7 (CCD7) and CCD8 play an important role. 9-cis-Epoxycarotenoid dioxygenase (NCED) is the most characterized CCD in plants, and a couple of NCED inhibitors including abamine and abamineSG were reported from our group. In this context we estimated that some of NCED inhibitors or their derivatives could inhibit strigolactone biosynthesis and we started to screen our chemical libraries targeted to NCED (CCD). In consequence we found that the treatment of abamine reduced the level of 5-deoxystrigol in root exudates from rice plants, while the treatment of abamineSG, a specific and potent inhibitor of NCED, did not reduced the level of 5-deoxystrigol. Now we continue screening strigolactone biosynthesis inhibitors and try to elucidate structure-activity relationship of abamine and its derivatives.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468323, CiNii Books ID：AN10348274
• 64. Characterization of strigolactones produced by chloronema of Physcomitrella patens and its ccd8 mutant　　　　　　　　　　　　　　　
  Sawabe Makiko; Xie Xiaonan; Yoneyama Kaori; Proust Helene; Hoffmann Beate; Rameau Catherine; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：44, First page：78, Last page：78, 06 Oct. 2009
  At the previous meeting, we reported that Physcomitrella patens subsp. patens, a bryophyte which is evolutionary most far from angiosperm, produces strigolactones (SLs). However, we often failed to detect SLs in P. patens grown in liquid culture. In this study, we collected only chloronema and analyzed SLs. The chloronema were obtained from 1-week old cultures of both WT and the CCD8 loss-of-function mutant (Ppccd8), and extracted with ethyl acetate. Characterization of SLs in these extracts was conducted by comparing retention times of germination stimulants on reversed phase HPLC with those of synthetic standards and by using LC-MS/MS. Both WT and Ppccd8 were found to produce SLs including 7-oxoorobanchyl acetate, 7α-hydroxyorobanchyl acetate, and orobanchyl acetate at similar levels. These results indicate that CCD8 homolog in P. patens would be redundant in the chloronema.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468324, CiNii Books ID：AN10348274
• 65. Characterization of strigolactones produced by rice plants　　　　　　　　　　　　　　　
  Harada Yuta; Yokoyama Mori; Xie Xiaonan; Yoneyama Kaori; Takeuchi Yasutomo; Cardoso Catarina; Charnikhova Tatsiana; Bouwmeester Harro; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：44, First page：79, Last page：79, 06 Oct. 2009
  Strigolactones (SLs) function in the rhizosphere as host detection signals for both symbionts arbuscular mycorrhizal (AM) fungi and root parasitic plants, and in the plants they act as a class of plant hormones inhibiting shoot branching. In this study, characterization of SLs produced by rice (Oryza sativa L. cv. Nipponbare) was conducted by comparing retention times of germination stimulants on reversed phase HPLC with those of synthetic standards and by LC-MS/MS. In the roots exudates collected from rice plants grown hydroponically, known SLs including 7-oxoorobanchyl acetate, 2'-epiorobanchol, orobanchol, orobanchyl acetate, and 2'-epi-5-deoxystrigol were detected. An unknown peak was detected in the channel for 7-oxoorobanchol (m/z 383>286) with distinct germination stimulation on Orobanche minor and Striga hermonthica seeds. LC-MS/MS and GC/MS analyses indicated that this is a novel SL. Purification and structural elucidation of the novel SL will be presented.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468325, CiNii Books ID：AN10348274
• 66. Identification and structure determination of strigolactones from cucumber root exudate　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Uchida Kenichi; Yokota Takao; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：44, First page：80, Last page：80, 06 Oct. 2009
  Strigolactone are plant secondary metabolites which function as host recognition signals for arbuscular mycorrhizal fungi and root parasitic plants, Striga and Orobanche. Recently, strigolactones have been identified as a novel class of plant hormones inhibiting shoots branching. In this study, characterization of strigolactones in cucumber (Cucumis sativus L.) root exudate was conducted. Cucumber was found to produce at least 9 germination stimulants including orobanchol, orobanchyl acetate, 7-oxoorobanchyl acetate, and 7-oxoorobanchol. In addition to these, two novel strigolactones were isolated and their structures were determined as 7α-hydroxy-orobanchyl acetate and 7β-hydroxy-orobanchyl acetate by 1D and 2D NMR spectroscopy, CD, and ESI- and EI-MS spectrometries.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468326, CiNii Books ID：AN10348274
• 9-34 イネのストリゴラクトン生産・分泌を制御するシグナル(9.植物の多量栄養素,2009年度京都大会)　　　　　　　　　　　　　　　
  米山 香織; 朝比奈 雅志; 野村 崇人; 米山 弘一; 横田 孝雄
  Volume：55, Number：55, First page：76, Last page：76, 15 Sep. 2009
  Japanese
  DOI：https://doi.org/10.20710/dohikouen.55.0_76_1
  DOI ID：10.20710/dohikouen.55.0_76_1, ISSN：0288-5840, CiNii Articles ID：110007719951
• Seed Germination : Physiology, Ecology and Molecular Mechanisms　　　　　　　　　　　　　　　
  BASKIN Carol C.; BASKIN Jerry M.; YOSHIOKA Toshihito; SEIWA Kenji; OZKAN Hakan; NONOGAKI Hiroyuki
  種生物学研究, Volume：32, First page：1, Last page：436, 31 Mar. 2009
  Japanese
  ISSN：0913-5561, CiNii Articles ID：10027795488, CiNii Books ID：AN10152669
• Signal Function of Plant Nutrition(Division 4: Plant Nutrition)　　　　　　　　　　　　　　　
  Sekimoto H; Yoneyama K
  Journal of the science of soil and manure, Japan, Volume：79, Number：6, First page：573, Last page：577, 13 Mar. 2009
  一般社団法人日本土壌肥料学会, Japanese
  DOI：https://doi.org/10.20710/dojo.79.6_573
  DOI ID：10.20710/dojo.79.6_573, ISSN：0029-0610, CiNii Articles ID：110007328266, CiNii Books ID：AN00195767
• 8B-1 Strigolactones, structural diversity and distribution in the plant kingdom　　　　　　　　　　　　　　　
  Yoneyama Koichi; Xie Xiaonan; Yoneyama Kaori; Takeuchi Yasutomo
  講演要旨集, Number：34, First page：36, Last page：36, 27 Feb. 2009
  日本農薬学会, Japanese
  CiNii Articles ID：110009761535, CiNii Books ID：AN00387542
• 9C22 Characterization of strigolactones produced and exuded by rice plants　　　　　　　　　　　　　　　
  Yoneyama Kaori; Xie Xianon; Takeuchi Yasutomo; Yokota Takao; Yoneyama Koichi
  講演要旨集, Number：34, First page：139, Last page：139, 27 Feb. 2009
  日本農薬学会, Japanese
  CiNii Articles ID：110009761635, CiNii Books ID：AN00387542
• Structural diversity and distribution of strigolactones in the plant kingdom　　　　　　　　　　　　　　　
  Koichi Yoneyama; Xiaonan Xie; Kaori Yoneyama; Yasutomo Takeuchi
  JOURNAL OF PESTICIDE SCIENCE, Volume：34, Number：4, First page：302, Last page：305, 2009
  被子植物の約1％（3000-4500種）は他の植物に寄生する寄生植物である。寄生植物のなかでも、農業生産に大きな被害を与えているのがハマウツボ科の根寄生雑草ストライガとオロバンキである。ストライガは光合成機能を有する半寄生性で、主にソルガム、トウモロコシ、サトウキビ、イネなどイネ科植物に寄生する。オロバンキは光合成機能を失った全寄生性で、主にトマト、ニンジン、タバコ、アブラナなどの双子葉植物に寄生する。これらの根寄生雑草は、巧妙な生存戦略によってその生息範囲を拡大している。それは、（1）大量の種子を生産し、（2）種子の寿命が長く、（3）種子発芽が宿主由来の化学物質によって誘導されることである。すなわち、根寄生雑草の種子は、宿主の根から分泌される発芽刺激物質にさらされて初めて発芽する。この発芽刺激物質には少なくとも3種類の化合物群、すなわち、ソルガムのジヒドロソルゴレオン、ヒマワリのセスキテルペンラクトン、そしてストリゴラクトン（SL）、が知られているが、最も多くの植物種が生産・分泌している発芽刺激物質がSLである。本稿では、SLの構造多様性と植物界における分布について解説する。
  PESTICIDE SCI SOC JAPAN, Japanese, Book review
  DOI：https://doi.org/10.1584/jpestics.34.302
  DOI ID：10.1584/jpestics.34.302, ISSN：1348-589X, eISSN：1349-0923, CiNii Articles ID：110007482302, Web of Science ID：WOS:000274116300011
• 1. Increased phosphate level in shoots retards production and exudation of strigolactones　　　　　　　　　　　　　　　
  Yoneyama Kaori; Sekimoto Hitoshi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：43, First page：15, Last page：15, 06 Oct. 2008
  Strigolactones are important host-recognition signals to commence parasitism by root parasitic weeds and mutualism by arbuscular mycorrhizal fungi and a novel class of plant hormones. Here we show that an increase of phosphate level but not the level in shoots retards production and exudation of strigolactones by using the root-split system of sorghum.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468175, CiNii Books ID：AN10348274
• 2. Isolation and structure determination of a novel strigolactone fabacyl acetate　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Harada Yuta; Fusegi Noria; Yamada Yoichi; Ito Satoshi; Yokota Takao; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：43, First page：16, Last page：16, 06 Oct. 2008
  Strigolactones are plant secondary metabolites that function as host recognition signals for arbuscular mycorrhizal fungi and root parasitic plants, Striga and Orobanche, and, in addition, plant hormones inhibiting shoot outgrowth. In this study, a novel strigolactone fabacyl acetate was purified from root exudates of pea (Pisum sativum L.) and its structure was determined as ent-2'-epi-4a,8a-epoxyorobanchyl acetate, by 1D and 2D NMR spectroscopy, ESI- and EI-MS spectrometry, X-ray crystallography, and by comparing ^1H NMR spectral data and retention times in LC-MS and GC-MS with those of synthetic standards prepared from (+)-orobanchol and (+)-2'-epiorobanchol. The ^1H NMR spectral data and retention times of fabacyl acetate were identical with those of an isomer prepared from (+)-2'-epiorobanchol except for the opposite sign in CD spectra.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468176, CiNii Books ID：AN10348274
• 3. Characterization of strigolactones produced by Physcomitrella patens subsp. patens　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Harada Yuta; Sekine Megumi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：43, First page：17, Last page：17, 06 Oct. 2008
  Strigolactones produced by a moss, Physcomitrella patens subsp. patens, were examined. Major strigolactones found in the ethyl acetate extract of the moss plants were identified as orobanchyl acetate, 7-oxoorobanchyl acetate, 7-hydroxyorobanchyl acetate, and ent-2'-epi-4a,8a-epoxyorobanchyl acetate (fabacyl acetate), by both ESI-LC/MS and GC/MS analyses. In addition to these strigolactones, orobanchol and sorgomol were detected by ESI-LC/MS. These results indicate that Physcomitrella patens is a good model plant for the study on biosynthesis of strigolactones.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468177, CiNii Books ID：AN10348274
• 4. Characterization of strigolactones produced by onion　　　　　　　　　　　　　　　
  Harada Yuta; Xie Xiaonan; Yoneyama Kaori; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：43, First page：18, Last page：18, 06 Oct. 2008
  Plants belong to the Liliacae family such as onion depend largely on symbiosis with arbuscular mycorrhizal (AM) fungi for the supply of mineral nutrients due to their rather poor root systems. In this study, characterization of strigolactones produced by onion (Allium cepa L.) was conducted by comparing retention times of germination stimulants on ODS-HPLC with those of synthetic standards and by LC/MS/MS. The root exudates from onion plants were found to contain at least 4 strigolactones, orobanchyl acetate, 7-oxoorobanchyl acetate, ent-2'-epi-4a,8a-epoxyorobanchyl acetate (fabacyl acetate), and 5-deoxystrigol.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468178, CiNii Books ID：AN10348274
• 5. Characterization of strigolactones produced and exuded by rice　　　　　　　　　　　　　　　
  Yoneyama Kaori; Xie Xiaonan; Harada Yuta; Matsuyama Mayumi; Sekine Megumi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：43, First page：19, Last page：19, 06 Oct. 2008
  Recently, strigolactones that were previously known as germination stimulants of root parasitic plants and hyphal branching factors of symbiotic arbuscular mycorrhizal fungi, have been shown to be a novel class of plant hormones inhibiting shoot outgrowth. In this study, characterization of strigolactones in the root exudates from 4 cultivars of rice plants grown hydroponically was conducted by comparing retention times of germination stimulants on reversed phase HPLC with those of synthetic and natural standards and by using LC/MS/MS.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468179, CiNii Books ID：AN10348274
• 7. Screening of strigolactone biosynthesis inhibitors　　　　　　　　　　　　　　　
  Ito Shinsaku; Kato Atsutaka; Hanada Atsushi; Yoneyama Kaori; Kitahata Nobutaka; Umehara Mikihisa; Suzuki Yoshihito; Yoneyama Koichi; Yamaguchi Shinjiro; Asami Tadao
  植物化学調節学会研究発表記録集, Number：43, First page：21, Last page：21, 06 Oct. 2008
  Shoot branching plays an important role in plant morphogenesis. Previous studies have suggested the novel branching hormone derived from carotenoids inhibit bud outgrowth. Recently, it has been reported that strigolactones (SL) act as a novel hormone in shoot branching. Strigolactones are rhizosphere signaling for the root parasitic weeds and symbiotic arbuscular mycorrhizal fungi. In the proposed biosynthesis pathway, carotenoid cleavage dioxygenase 7 (CCD7) and CCD8 are involved in SL biosynthesis and catalyse cartenoid cleavage reaction. 9-cis-Epoxycarotenoid dioxygenase (NCED) is the most characterized CCD in plants, and we previously identified some NCED inhibitors. To design a strigolactone biosynthesis inhibitor, we screened our chemical libraries targeted to NCED and found abamine, previously identified as NCED inhibitor, reduced the level of 5-deoxystrigol in root exudates. However abamineSG, a specific and potent inhibitor of NCED, did not reduced the level of 5-deoxystrigol.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110007468181, CiNii Books ID：AN10348274
• 8-1 養分条件が植物のストリゴラクトン生産・分泌に与える影響(8.共生,2008年度愛知大会)　　　　　　　　　　　　　　　
  米山 香織; 関本 均; 竹内 安智; 米山 弘一
  Number：54, First page：57, Last page：57, 09 Sep. 2008
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110007456609, CiNii Books ID：AN00195778
• Production of Strigolactones by Arabidopsis thaliana responsible for Orobanche aegyptiaca seed germination　　　　　　　　　　　　　　　
  Yaakov Goldwasser; Kaori Yoneyama; Xiaonan Xie; Koichi Yoneyama
  PLANT GROWTH REGULATION, Volume：55, Number：1, First page：21, Last page：28, May 2008
  The germination stimulants produced by Arabidopsis thaliana, a host of root parasitic plants Orobanche spp. but not of arbuscular mycorrhizal (AM) fungi were examined. Root exudates from the hydroponically grown A. thaliana plants were subjected to reverse phase high performance liquid chromatography (HPLC) and retention times of germination stimulants inducing O. aegyptiaca seed germination were compared with those of strigolactone standards. In addition, the root exudates were analyzed by using HPLC linked with tandem mass spectrometry (LC/MS/MS). A. thaliana was found to exude at least three different germination stimulants of which one was identified as orobanchol. This is the first report of strigolactone production by a non-mycotrophic plant. These results together with recent knowledge imply that strigolactones have other unrevealed functions in plant growth and development.
  SPRINGER, English
  DOI：https://doi.org/10.1007/s10725-008-9253-z
  DOI ID：10.1007/s10725-008-9253-z, ISSN：0167-6903, CiNii Articles ID：80019452661, Web of Science ID：WOS:000254460700003
• C216 How mineral nutrients affect the production and/or exudation of strigolactones　　　　　　　　　　　　　　　
  Yoneyama Kaori; Sekimoto Hitoshi; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Volume：33, Number：33, First page：80, Last page：80, 11 Mar. 2008
  日本農薬学会, Japanese
  CiNii Articles ID：110006858219, CiNii Books ID：AN00387542
• C217 Evaluation of strigolactones for seed germination stimulation of root parasitic plants　　　　　　　　　　　　　　　
  Kim Hyun-il; Yoneyama Kaori; Xie Xiaonan; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Volume：33, Number：33, First page：81, Last page：81, 11 Mar. 2008
  日本農薬学会, Japanese
  CiNii Articles ID：110006858220, CiNii Books ID：AN00387542
• C218 Germination stimulants for root parasitic plants produced by cucumber plants　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Kusumoto Dai; Kim Hyun-il; Yamada Yoichi; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Volume：33, Number：33, First page：82, Last page：82, 11 Mar. 2008
  日本農薬学会, Japanese
  CiNii Articles ID：110006858221, CiNii Books ID：AN00387542
• 第19回国際植物生長物質会議に参加して(5)(第19回国際植物生長物質会議派遣補助金受賞者報告,談話室)　　　　　　　　　　　　　　　
  米山 香織
  Volume：42, Number：2, First page：191, Last page：192, 20 Dec. 2007
  Japanese
  DOI：https://doi.org/10.18978/jscrp.42.2_191_3
  DOI ID：10.18978/jscrp.42.2_191_3, ISSN：1346-5406, CiNii Articles ID：110006533792
• The 19th IPGSA Conference report　　　　　　　　　　　　　　　
  HNINSI Moe
  Regulation of plant growth & development, Volume：42, Number：2, First page：190, Last page：195, 20 Dec. 2007
  植物化学調節学会, Japanese
  ISSN：1346-5406, CiNii Articles ID：10020225314, CiNii Books ID：AA11550064
• ii. Weed Control(1. Chemistry Including Natural Products, Poster Session Category I)　　　　　　　　　　　　　　　
  森本 正則; 謝 肖男; 上岡 弘明; 米山 香織
  Journal of pesticide science, Volume：32, Number：0, First page：S82, Last page：S84, 10 Oct. 2007
  日本農薬学会, Japanese
  ISSN：1348-589X, CiNii Articles ID：110006453223, CiNii Books ID：AA11818622
• Isolation and structure determination of a novel strigolactone, (+)-sorgomol, from sorghum root exudate　　　　　　　　　　　　　　　
  XIE Xiaonan; YONEYAMA Kaori; KUSUMOTO Dai; YAMADA Yoichi; TAKEUCHI Yasutomo; YONEYAMA Koichi
  Volume：42, First page：74, Last page：74, 05 Oct. 2007
  Sorghum (Sorghum bicolor) is an important food crop in Africa and a host of the devastating root parasitic weed Striga hermonthica. Seeds of root parasitic weeds germinate only when they are exposed to exogenous germination stimulants produced by and released from roots of host and some non-host plants. Sorghum has already been shown to exude sorgolactone, strigol, and 5-deoxystrigol. In addition to these known strigolactones, an isomer of strigol was identified in root exudates of sorghum cultivars M800 and Hybrid. This novel strigolactone, named sorgomol, was isolated and its structure was determined as (3aR,8R,8bS,E)-8-(hydroxymethyl)-8-methyl-3-(((R)-4-memyl-5-oxo-2,5-dihydromran-2-yloxy)methyleme)-3a,4,5,6,7,8-hexahydro-3H-indeno[1,2-b]furan-2(8bH)-one by 1D and 2D NMR spectroscopy and ESI- and EI-MS spectrometry.
  The Japanese Society for Chemical Regulation of Plants, Japanese
  ISSN：1346-5406, CiNii Articles ID：110006474754, CiNii Books ID：AA11550064
• Characterization of strigolactones, host recognition signals for arbuscular mycorrhizal fungi and root parasitic plants, produced by pea　　　　　　　　　　　　　　　
  HARADA Yuta; XIE Xiaonan; KUSUMOTO Dai; FUSEGI Norio; YONEYAMA Kaori; TAKEUCHI Yasutomo; YAMADA Yoichi; YOKOTA Takao; YONEYAMA Koichi
  Volume：42, First page：75, Last page：75, 05 Oct. 2007
  Strigolactones are plant secondary metabolites which function as host recognition signals for arbuscular mycorrhizal (AM) fungi and root parasitic plants, Striga and Orobanche. Plants exudates cocktails of strigolactones and their compositions differ with plant species, their growth stages, and growth conditions. In this study, characterization of strigolactones produced by pea (Pisum sativum L.), a host of Orobanche, was conducted by comparing retention times of germination stimulants on ODS-HPLC with those of synthetic standards and by LC/MS/MS. In the root exudates collected from pea plants grown hydroponically, orobanchol, orobanchyl acetate, and a novel strigolactone were detected as major stimulants. Purification and structural elucidation of the novel strigolactone will be presented.
  The Japanese Society for Chemical Regulation of Plants, Japanese
  ISSN：1346-5406, CiNii Articles ID：110006474755, CiNii Books ID：AA11550064
• 61. Isolation and structure determination of a novel strigolactone, (+)-sorgomol, from sorghum root exudate　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Kusumoto Dai; Yamada Yoichi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：42, Number：42, 05 Oct. 2007
  Sorghum (Sorghum bicolor) is an important food crop in Africa and a host of the devastating root parasitic weed Striga hermonthica. Seeds of root parasitic weeds germinate only when they are exposed to exogenous germination stimulants produced by and released from roots of host and some non-host plants. Sorghum has already been shown to exude sorgolactone, strigol, and 5-deoxystrigol. In addition to these known strigolactones, an isomer of strigol was identified in root exudates of sorghum cultivars M800 and Hybrid. This novel strigolactone, named sorgomol, was isolated and its structure was determined as (3aR,8R,8bS,E)-8-(hydroxymethyl)-8-methyl-3-(((R)-4-memyl-5-oxo-2,5-dihydromran-2-yloxy)methyleme)-3a,4,5,6,7,8-hexahydro-3H-indeno[1,2-b]furan-2(8bH)-one by 1D and 2D NMR spectroscopy and ESI- and EI-MS spectrometry.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110006474754
• 62. Characterization of strigolactones, host recognition signals for arbuscular mycorrhizal fungi and root parasitic plants, produced by pea　　　　　　　　　　　　　　　
  Harada Yuta; Xie Xiaonan; Kusumoto Dai; Fusegi Norio; Yoneyama Kaori; Takeuchi Yasutomo; Yamada Yoichi; Yokota Takao; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：42, Number：42, 05 Oct. 2007
  Strigolactones are plant secondary metabolites which function as host recognition signals for arbuscular mycorrhizal (AM) fungi and root parasitic plants, Striga and Orobanche. Plants exudates cocktails of strigolactones and their compositions differ with plant species, their growth stages, and growth conditions. In this study, characterization of strigolactones produced by pea (Pisum sativum L.), a host of Orobanche, was conducted by comparing retention times of germination stimulants on ODS-HPLC with those of synthetic standards and by LC/MS/MS. In the root exudates collected from pea plants grown hydroponically, orobanchol, orobanchyl acetate, and a novel strigolactone were detected as major stimulants. Purification and structural elucidation of the novel strigolactone will be presented.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110006474755
• 63. How mineral nutrients affect the production and exudation of strigolactones by sorghum　　　　　　　　　　　　　　　
  Yoneyama Kaori; Sekimoto Hitoshi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：42, Number：42, 05 Oct. 2007
  Strigolactones are important host-recognition chemicals to commence parasitism by root parasitic weeds and mutualism by arbuscular mycorrhizal (AM) fungi. We already reported that effects of nutrient deficiencies on strigolactone exudation vary with plant species. In this study, the effects of mineral nutrients on the production and exudation of 5-deoxystrigol, one of main strigolactones exuded by sorghum, were examined in detail.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110006474756
• 植物の根に関する諸問題(170)根が分泌する根寄生植物およびAM菌の宿主認識シグナル　　　　　　　　　　　　　　　
  米山 香織; 関本 均
  Volume：82, Number：9, First page：1025, Last page：1030, Sep. 2007
  Japanese
  ISSN：0369-5247, CiNii Articles ID：40015609659, CiNii Books ID：AN0038751X
• 8-22 養分条件がソルガムのストリゴラクトン-AM菌と根寄生植物の宿主認識シグナル-生産・分泌に与える影響(8.共生,2007年度東京大会)　　　　　　　　　　　　　　　
  米山 香織; 関本 均; 竹内 安智; 米山 弘一
  Number：53, First page：59, Last page：59, 22 Aug. 2007
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110006598253, CiNii Books ID：AN00195778
• 30 ホスト植物が同化した炭素・窒素の根寄生植物への分配様式のPETIS法によるイメージング(関東支部講演会,2006年度各支部会)　　　　　　　　　　　　　　　
  本田 修三; 関本 均; 加藤 翔太; 落合 由記子; 山口 良恵; 米山 香織; 米山 弘一; 竹内 安智; 河地 有木; 藤巻 秀; 鈴井 伸郎; 石井 里美; 石岡 典子; 松橋 信平
  Volume：53, Number：53, 22 Aug. 2007
  Japanese
  ISSN：0288-5840, J-Global ID：200902235237404008, CiNii Articles ID：110006598782
• C306 Novel germination stimulants for root parasitic plants produced by flax plants　　　　　　　　　　　　　　　
  Xie Xiaonan; Yoneyama Kaori; Kusumoto Dai; Kurita Jun-ya; Yamada Yoichi; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Volume：32, Number：32, First page：81, Last page：81, 09 Mar. 2007
  日本農薬学会, Japanese
  CiNii Articles ID：110006372562, CiNii Books ID：AN00387542
• How mineral nutrients affect the production of strigolactones by plants　　　　　　　　　　　　　　　
  YONEYAMA Kaori; SEKIMOTO Hitoshi; TAKEUCHI Yasutomo; YONEYAMA Koichi
  Volume：41, First page：69, Last page：69, 04 Oct. 2006
  The Japanese Society for Chemical Regulation of Plants, Japanese
  ISSN：1346-5406, CiNii Articles ID：110006168816, CiNii Books ID：AA11550064
• 54.How mineral nutrients affect the production of strigolactones by plants　　　　　　　　　　　　　　　
  Yoneyama Kaori; Sekimoto Hitoshi; Takeuchi Yasutomo; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Volume：41, Number：41, 04 Oct. 2006
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110006168816
• P8-1 栄養条件は宿主植物の寄生・共生開始シグナル分泌に影響する(8. 共生, 2006年度秋田大会講演要旨)　　　　　　　　　　　　　　　
  米山 香織; 竹内 安智; 米山 弘一; 関本 均
  Number：52, First page：46, Last page：46, 05 Sep. 2006
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110006287440, CiNii Books ID：AN00195778
• 11 ^<13>Nを用いたホスト植物根吸収窒素の根寄生植物による収奪の可視化と収奪率の定量解析(関東支部講演会, 2005年度各支部会講演要旨)　　　　　　　　　　　　　　　
  本田 修三; 関本 均; 松木 良祐; 米山 香織; 落合 由記子; 米山 弘一; 竹内 安智; 松橋 信平; 河地 有木; 藤巻 秀; 阪本 浩一; 鈴井 伸郎; 石井 里美; 石岡 典子; 渡辺 智; 荒川 和夫; 久米 民和
  Number：52, First page：259, Last page：259, 05 Sep. 2006
  Japanese
  ISSN：0288-5840, CiNii Articles ID：110006288010, CiNii Books ID：AN00195778
• C203 Strigolactones, germination stimulants for root parasitic plants, produced by and released from dicotyledonous plants　　　　　　　　　　　　　　　
  Yoneyama Kaori; Sekimoto Hitoshi; Takeuchi Yasutomo; Yoneyama Koichi
  講演要旨集, Volume：31, Number：31, First page：76, Last page：76, 10 Mar. 2006
  日本農薬学会, Japanese
  CiNii Articles ID：110006372451, CiNii Books ID：AN00387542
• 11. The response to phosphorus availability of the production and/or exudation of orobanchol, germination stimulant for root parasitic plants, in red clover　　　　　　　　　　　　　　　
  Yoneyama Kaori; Takeuchi Yasutomo; Yoneyama Koichi; Sekimoto Hitoshi
  植物化学調節学会研究発表記録集, Number：40, First page：30, Last page：30, 13 Oct. 2005
  Among mineral nutrients, reduced supply of phosphorus significantly enhanced production and/or exudation of the germination stimulant orobanchol for Orobanche minor by red clover plants grown hydroponically. Therefore, response of orobanchol exudation to phosphorus availability was examined in detail. Orobanchol exudation greatly increased 48 hours after transferring to low P condition (8μM P). The plants exuded more orobanchol when grown in media with lower P levels. When the plants were continuously grown at 8μM P, orobanchol exudation continued to increase further with incubation period. By contrast, when the plants grown at 8μM P were transferred to 80 or 160μM P, orobanchol exudation decreased within the first 24 hours. These data suggest that red clover plants sense and respond quickly to changes of phosphorus availability in soil and regulate biosynthesis and/or exudation of strigolactones.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110002552709, CiNii Books ID：AN10348274
• 42. Genes involved in biosynthesis and metabolism of germination stimulants, strigolactones　　　　　　　　　　　　　　　
  Fukasawa Masashi; Matsukubo Tomo; Yoneyama Kaori; Iino Mayumi; Goda Hideki; Asami Tadao; Yoshida Shigeo; Takeuchi Yasutomo; Nakano Takeshi; Yoneyama Koichi
  植物化学調節学会研究発表記録集, Number：40, First page：61, Last page：61, 13 Oct. 2005
  Root parasitic plants depend on their hosts for water and nutrients. The seeds of these parasites germinate when they receive stimulants secreted from roots of host and some non-host plants. To date, several germination stimulants, collectively called strigolactones, have been isolated, but their biosynthetic pathway has not been clarified. Recently, Arabidopsis thaliana was shown to be susceptible to root parasites including some Orobanche species and we have demonstrated that one of major germination stimulants produced by Arabidopsis is orobanchol. Therefore, microarray analysis was performed to identify genes involved in biosynthesis and metabolism of strigolactones in Arabidopsis. Two new P450 oxidases with unknown functions and an ABC transporter were identified as highly responsive to strigol treatment. Further analyses with using knock-out mutants of these genes are now in progress.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110002552740, CiNii Books ID：AN10348274
• Effects of nutrients on the production of germination stimulants for root parasitic plants by red clover　　　　　　　　　　　　　　　
  YONEYAMA Kaori; TAKEUCHI Yasutomo; YONEYAMA Koichi; SEKIMOTO Hitoshi
  Volume：39, First page：58, Last page：58, 28 May 2005
  Orobanche species causing enormous damage to the agriculture prevail on nutrient deficient soils and their emergence is often suppressed by the application of fertilizers. However, it has not been clarified yet whether nutrients affect the production of germination stimulants or that of germination inhibitors. Therefore in this study, the amount of orobanchol, one of germination stimulants for O. minor Sm., in the root exudates of its host red clover (Trifolium pratense L. cv. Makimidori) plants grown hydroponically under various low nutrient levels (N, P, K, Ca, and Mg) were quantified by using LC/MS/MS. In addition, germination stimulant activities of the root exudates were also assessed by bioassays.
  The Japanese Society for Chemical Regulation of Plants, Japanese
  ISSN：1346-5406, CiNii Articles ID：110002552652, CiNii Books ID：AA11550064
• 37. Effects of nutrients on the production of germination stimulants for root parasitic plants by red clover　　　　　　　　　　　　　　　
  Yoneyama Kaori; Takeuchi Yasutomo; Yoneyama Koichi; Sekimoto Hitoshi
  植物化学調節学会研究発表記録集, Volume：39, Number：39, 13 Oct. 2004
  Orobanche species causing enormous damage to the agriculture prevail on nutrient deficient soils and their emergence is often suppressed by the application of fertilizers. However, it has not been clarified yet whether nutrients affect the production of germination stimulants or that of germination inhibitors. Therefore in this study, the amount of orobanchol, one of germination stimulants for O. minor Sm., in the root exudates of its host red clover (Trifolium pratense L. cv. Makimidori) plants grown hydroponically under various low nutrient levels (N, P, K, Ca, and Mg) were quantified by using LC/MS/MS. In addition, germination stimulant activities of the root exudates were also assessed by bioassays.
  植物化学調節学会, Japanese
  ISSN：0919-1887, CiNii Articles ID：110002552652

• 植物の多次元コミュニケーションダイナミクス : 分子メカニズムから農業応用の可能性まで　　　　　　　　　　　　　　　
  米山香織, [Joint work]
  Feb. 2025
  Japanese, Total pages：2, 8, 346, 10p, 図版 34p
  CiNii Books：http://ci.nii.ac.jp/ncid/BD10554773
  ISBN：9784860439422, CiNii Books ID：BD10554773

• JAPANESE SOCIETY OF SOIL SCIENCE AND PLANT NUTRITION
• PESTICIDE SCIENCE SOCIETY OF JAPAN
• JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY
• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS
• THE JAPANESE SOCIETY FOR CHEMICAL REGULATION OF PLANTS

• 真の枝分かれ抑制ホルモンの化学構造、生理機能、生合成経路の解明　　　　　　　　　　　　　　　
  01 Apr. 2021 - 31 Mar. 2026
  Grant amount(Total)：16770000, Direct funding：12900000, Indirect funding：3870000
  Grant number：21H02125
• 未知ストリゴラクトン代謝経路におけるHIS1/HSLファミリーの機能的役割の解明　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2025
  Grant amount(Total)：17420000, Direct funding：13400000, Indirect funding：4020000
  Grant number：22H02270
• Elucidation of the germination controlling mechanism of root parasitic plants by tryptophan related chemicals　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), 01 Apr. 2022 - 31 Mar. 2025
  Meiji University
  Grant amount(Total)：17680000, Direct funding：13600000, Indirect funding：4080000
  Grant number：22H02276
• Regulation of strigolactone biosynthesis in Arabidopsis　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2019 - 31 Mar. 2022
  YONEYAMA KAORI, Ehime University
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  In Arabidopsis thaliana (Col-0), nitrogen deficiency significantly promoted the expression of strigolactone (SL) biosynthetic genes. In Atd14 mutants, carlactone and methyl carlactonoate, major SLs of Arabidopsis, accumulated under nitrogen deficiency. Furthermore, the expression of MAX1, which is involved in the conversion of carlactone into carlactonoic acid, increased within 3 hours after beginning of light period. These results suggest that for successful SL detection/quantification in Arabidopsis, it is better to grow plants under nitrogen deficiency and harvest them within 3 hours after beginning of light period.
  Grant number：19K05757
• ストリゴラクトン生産・分泌制御を介したアーバスキュラー菌根菌利用技術の確立　　　　　　　　　　　　　　　
  2017 - 2021
  Principal investigator
• Characterization of shoot-branching inhibiting hormones, their functions and biosynthesis　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2016 - 31 Mar. 2019
  YONEYAMA KOICHI, Utsunomiya University
  Grant amount(Total)：17940000, Direct funding：13800000, Indirect funding：4140000
  Structure- and stereospecific transport of strigolactones (SLs) from roots to shoots suggested that true shoot branching inhibitory hormones are not canonical-SLs such as strigol and orobanchol. For example, in rice plants, an orobanchol-type SL producer, strigol applied to roots inhibited tillering (shoot branching) but was not detected in shoots harvested 20 hours after treatment. Two carlactone (CL) derivatives were detected in the xylem saps from different plant species including rice, tomato, pumpkin, and cucumber, and their structures were found to be hydroxycalactonoic acid and its methyl ester based on the comparison of their LC-MS/MS data with those of synthetic standards. Unfortunately, we could not isolate pure compounds enough for NMR measurements and thus their structures have not yet confirmed. Further studies are needed to ambiguously determine their structures and confirm their biological functions.
  Grant number：16H04914
• Function of novel strigolactone biosynthesis genes in Arabidopsis　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), 01 Apr. 2016 - 31 Mar. 2019
  Yoneyama Kaori
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  Strigolactone (SL) is a shoot branching inhibitory plant hormone. The objective of this study is to elucidate the SL biosynthetic pathway and active shoot branching inhibitor by clarifying the function of novel SL biosynthetic genes. We reported that carlactone (CL) and methyl carlactonoate (MeCLA), SL intermediates accumulate in Arabidopsis lbo mutants and MeCLA is consumed as a substrate and [MeCLA+16 Da] compound is produced by recombinant LBO protein expressed in E. coli in PNAS paper (Brewer et al. 2016). Then, we found that [MeCLA+16 Da] is hydroxyMeCLA and exists in Arabidopsis SL insensitive mutants of Atd14 and not in lbo mutants (Yoneyama et al. in preparation). We also reported the function of MAX1 homologs, working upstream of LBO, in important major crops, model tree popular and model lower plant fern in New Phytologist (Yoneyama et al. 2018).
  Grant number：16K18560
• Strigolactone biosynthesis and its regulation mechanism in plant　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (A)), 2019 - 2019
  Ehime University
  Grant amount(Total)：13910000, Direct funding：10700000, Indirect funding：3210000
  Grant number：19KK0395
• ストリゴラクトン生合成の調節メカニズムに関する研究　　　　　　　　　　　　　　　
  24 Apr. 2015 - 31 Mar. 2018
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  Grant number：15J40043
• ストリゴラクトンの生理機能とその調節メカニズムに関する研究　　　　　　　　　　　　　　　
  2010 - 2012
  Grant amount(Total)：3482532, Direct funding：3482532
  Grant number：10J09996