TOMIOKA Hiroaki
| Natural science course | Professor |
| Graduate School of Education | |
Performance information
■ MISC非平衡条件下で分子集合体が示す動的非線形現象を用いて科学の面白さを伝えるための教材及び演示法の開発とその実践
冨岡寛顕
Volume：第5号(18年度), First page：137, Last page：138, 2007
非平衡条件下で分子集合体が示す動的非線形現象を用いて科学の面白さを伝えるための教材及び演示法の開発とその実践
冨岡寛顕
総合研究機構研究プロジェクト研究成果報告書, Volume：第5号(18年度), First page：137, Last page：138, 2007
A Simple and Fast Method for Demonstrating the Photocatalytic Reaction by TiO_2 YAMAMOTO Kouki; TOMIOKA Hiroaki
CHEMISTRY & EDUCATION,
Volume：54,
Number：12,
First page：668,
Last page：671, 2006
The Chemical Society of Japan, Japanese
DOI：https://doi.org/10.20665/kakyoshi.54.12_668DOI ID：10.20665/kakyoshi.54.12_668,
ISSN：0386-2151,
CiNii Articles ID：110008906955,
CiNii Books ID：AN10033386 酸化チタンの光触媒作用を短時間で演示教材の開発ー寒天ゲル固定化酸化チタン（ＴｉＯ２）による短時間色素分解 山本孔紀; 冨岡寛顕
化学と教育,
Volume：54,
Number：12,
First page：668,
Last page：671, 2006
The Chemical Society of Japan, Japanese
DOI：https://doi.org/10.20665/kakyoshi.54.12_668DOI ID：10.20665/kakyoshi.54.12_668,
ISSN：0386-2151,
CiNii Articles ID：110008906955,
CiNii Books ID：AN10033386 Detection and expression of a gene encoding a new bacteriorhodopsin from an extreme halophile strain HT (JCM 9743) which does not possess bacteriorhodopsin activity M Kamekura; Y Seno; H Tomioka
EXTREMOPHILES,
Volume：2,
Number：1,
First page：33,
Last page：39, Jan. 1998
Membrane vesicles prepared from an extreme halophile strain, HT (JCM 9743), showed no bacteriorhodopsin activity. However, a DNA fragment, amplified by polymerase chain reaction (PCR), appeared to encode the C to G helices of a bacteriorhodopsin(bR)-like protein. With the PCR product as a probe, the gene coding for a novel bacteriorhodopsin was cloned from the genomic DNA of the strain HT. The open reading frame of the gene was ligated with the promoter region of the bop gene of Halobacterium salinarum bR, and expressed in a bR-deficient host strain, L33, using the plasmid vector pXLNov-R. The purplish membrane fraction purified from cells of a transformant exhibited a cyclic photoreaction characteristic of bacteriorhodopsin.
SPRINGER VERLAG, English
DOI：https://doi.org/10.1007/s007920050040DOI ID：10.1007/s007920050040,
ISSN：1431-0651,
CiNii Articles ID：80010133349,
PubMed ID：9676241,
Web of Science ID：WOS:000072044400005 Detection and expression of a gene encoding a new bacteriorhodopsin from an extreme halophile strain HT (JCM 9743) which does not possess bacteriorhodopsin activity M Kamekura; Y Seno; H Tomioka
EXTREMOPHILES,
Volume：2,
Number：1,
First page：33,
Last page：39, Jan. 1998
Membrane vesicles prepared from an extreme halophile strain, HT (JCM 9743), showed no bacteriorhodopsin activity. However, a DNA fragment, amplified by polymerase chain reaction (PCR), appeared to encode the C to G helices of a bacteriorhodopsin(bR)-like protein. With the PCR product as a probe, the gene coding for a novel bacteriorhodopsin was cloned from the genomic DNA of the strain HT. The open reading frame of the gene was ligated with the promoter region of the bop gene of Halobacterium salinarum bR, and expressed in a bR-deficient host strain, L33, using the plasmid vector pXLNov-R. The purplish membrane fraction purified from cells of a transformant exhibited a cyclic photoreaction characteristic of bacteriorhodopsin.
SPRINGER VERLAG, English
DOI：https://doi.org/10.1007/s007920050040DOI ID：10.1007/s007920050040,
ISSN：1431-0651,
CiNii Articles ID：80010133349,
PubMed ID：9676241,
Web of Science ID：WOS:000072044400005 Voltage-dependent absorbance change of carotenoids in halophilic archaebacteria SI Seki; H Sasabe; H Tomioka
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,
Volume：1284,
Number：1,
First page：79,
Last page：85, Oct. 1996
Membrane vesicles of wild-type Halobacterium sp. mex strain show a wavy absorbance change which has not been so far reported in halophilic archaebacteria. A white mutant strain lacking carotenoids did not show the wavy absorbance change. The wavy absorbance change in the range of 440-590 nm was induced by a red flash (600-640 nm), which photoexcited electrogenic ion pumps, mex bacteriorhodopsin and mex halorhodopsin but not carotenoids. The wavy change was also caused by K+ diffusion potentials without light. These results suggest that the wavy absorbance change in the membrane vesicles is the voltage-dependent absorbance change of the carotenoids.
ELSEVIER SCIENCE BV, English
DOI：https://doi.org/10.1016/0005-2736(96)00114-9DOI ID：10.1016/0005-2736(96)00114-9,
ISSN：0005-2736,
eISSN：0006-3002,
Web of Science ID：WOS:A1996VM11500013 Real-time image processing using polarization discrimination of bacteriorhodopsin Y OkadaShudo; Yamaguchi, I; H Tomioka; H Sasabe
SYNTHETIC METALS,
Volume：81,
Number：2-3,
First page：147,
Last page：149, Aug. 1996
The application of biological photochrome bacteriorhodopsin (bR) films for real-time image processing is described; bR exhibits strong polarization-sensitive phase conjugation. We demonstrate exclusive OR operation with polarization properties of the phase conjugate (PC) beam in degenerated four-wave mixing (DFWM). Also, real-time image subtraction and addition can be performed by transmitting two mutually orthogonal polarized phase conjugate beams through an analyzer.
ELSEVIER SCIENCE SA, English
DOI：https://doi.org/10.1016/S0379-6779(96)03757-5DOI ID：10.1016/S0379-6779(96)03757-5,
ISSN：0379-6779,
CiNii Articles ID：80009297691,
Web of Science ID：WOS:A1996VV10900010
■ Research projects 
