Performance information

• Defective Pollen Meiosis in Arabidopsis Due to Combined Arabinan and Galactan Insufficiency
  Takuma Kikuchi; Kouichi Soga; Toshihisa Kotake; Daisuke Takahashi
  Plant And Cell Physiology, First page：in press, Jul. 2025, [Reviewed]
  Abstract
  
  The development of pollen is critical for seed plants and depends on precise cellular and molecular mechanisms. It is known that the cell wall plays a key role in the progression of pollen maturation. An earlier phase in pollen development is the meiosis of pollen mother cells (PMCs), a fundamental process for producing viable pollen grains. However, the significance of the cell wall during pre-meiosis processes has remained unclear. Pectin, a major component of the cell wall, accumulates abundantly in pollen. To investigate the significance of the cell wall during pre-meiosis and meiosis, we generated an arad1 gals2 gals3 triple mutant of Arabidopsis thaliana, lacking the genes responsible for normal synthesis of arabinan and galactan, which constitute the side chains of pectin. Although vegetative growth and cell wall properties were comparable in wild-type (WT) and arad1 gals2 gals3, the pollen development in the mutant failed during meiosis. Immunohistochemical analysis showed that pectic arabinan and galactan accumulated in WT PMCs before meiosis, but this was not observed in mutant PMCs. On the other hand, it was found that pistil development of WT and arad1 gals2 gals3 was comparable. These findings suggest that the transient accumulation of arabinan and galactan in PMCs before meiosis is crucial for pollen meiosis. We believe that this study is the first to demonstrate the critical role of cell wall components, specifically pectic arabinan and galactan, in the pre-meiosis processes of pollen development.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcaf085
  DOI ID：10.1093/pcp/pcaf085, ISSN：0032-0781, eISSN：1471-9053
• Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls
  Yoshihisa Yoshimi; Li Yu; Rosalie Cresswell; Xinyu Guo; Alberto Echevarría-Poza; Jan J. Lyczakowski; Ray Dupree; Toshihisa Kotake; Paul Dupree
  Nature Communications, Volume：16, Number：1, Jan. 2025, [Reviewed]
  Abstract
  
  Widely found in most plant lineages, β-mannans are structurally diverse polysaccharides that can bind to cellulose fibrils to form the complex polysaccharide architecture of the cell wall. How changes in polysaccharide structure influence its cell wall solubility or promote appropriate interaction with cellulose fibrils is poorly understood. Glucomannan backbones acquire variable patterns of galactosyl substitutions, depending on plant developmental stage and species. Here, we show that fine-tuning of galactosyl modification on glucomannans is achieved by the differing acceptor recognition of mannan α-galactosyltransferases (MAGTs). Biochemical analysis and ¹³C solid-state nuclear magnetic resonance spectroscopy of Arabidopsis with cell wall glucomannan engineered by MAGTs reveal that the degree of galactosylation strongly affects the interaction with cellulose. The findings indicate that plants tailor galactosyl modification on glucomannans for constructing an appropriate cell wall architecture, paving the way to convert properties of lignocellulosic biomass for better use.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s41467-025-56626-y
  DOI ID：10.1038/s41467-025-56626-y, eISSN：2041-1723
• Soluble sugars make a greater contribution than cell wall components to the variability of freezing tolerance in wheat cultivars
  Sushan Chowhan; Takuma Kikuchi; Momoka Ohashi; Tatsuya Kutsuno; Hiroto Handa; Toshihisa Kotake; Daisuke Takahashi
  Plant Biotechnology, Volume：41, Number：4, First page：401, Last page：415, Dec. 2024, [Reviewed]
  Japanese Society for Plant Cell and Molecular Biology, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.24.0801a
  DOI ID：10.5511/plantbiotechnology.24.0801a, ISSN：1342-4580, eISSN：1347-6114
• SHORT AND CROOKED AWN, Encoding an Epigenetic Regulator EMF1, Promotes Barley Awn Development.　　　　　　　　　　　　　　　
  Koki Nakamura; Yuichi Kikuchi; Mizuho Shiraga; Toshihisa Kotake; Kiwamu Hyodo; Shin Taketa; Yoko Ikeda
  Plant & cell physiology, Dec. 2024, [Reviewed], ［Domestic magazine］
  The awn is a bristle-like extension from the lemma of grass spikelets. In barley, the predominant cultivars possess long awns that contribute to grain yield and quality through photosynthesis. Barley is a useful cereal crop to investigate the mechanism of awn development as various awn morphological mutants are available. Here, we identified the gene causative of the short and crooked awn (sca) mutant, which exhibits a short and curved awn phenotype. Intercrossing experiments revealed that the sca mutant induced in the Japanese cultivar (cv.) 'Akashinriki' is allelic to independently isolated moderately short-awn mutant breviaristatum-a (ari-a). Map-based cloning and sequencing revealed that SCA encodes the Polycomb group-associated protein EMBRYONIC FLOWER 1 (EMF1). We found that SCA affects awn development through the promotion of cell proliferation, elongation, and cell wall synthesis. RNA sequencing of cv. Bowman (BW) backcross-derived near-isogenic lines of sca and ari-a6 alleles showed that SCA is directly or indirectly involved in promoting the expression of genes related to awn development. Additionally, SCA represses various transcription factors essential for floral organ development and plant architecture, such as MADS-box and KNOX1 genes. Notably, the repression of the C-class MADS-box gene HvMADS58 by SCA in awns is associated with the accumulation of the repressive histone modification H3K27me3. These findings highlight the potential role of SCA-mediated gene regulation, including histone modification, as a novel pathway in barley awn development.
  Oxford University Press (OUP), English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcae150
  DOI ID：10.1093/pcp/pcae150, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：39704093
• Cytosolic UDP‐L‐arabinose synthesis by bifunctional UDP‐glucose 4‐epimerases in Arabidopsis
  Akira Umezawa; Mayuko Matsumoto; Hiroto Handa; Konatsu Nakazawa; Megumi Miyagawa; Georg J. Seifert; Daisuke Takahashi; Shinya Fushinobu; Toshihisa Kotake
  The Plant Journal, Volume：118, First page：508, Last page：524, Apr. 2024, [Reviewed], [Last, Corresponding]
  SUMMARY
  
  L‐Arabinose (L‐Ara) is a plant‐specific sugar found in cell wall polysaccharides, proteoglycans, glycoproteins, and small glycoconjugates, which play physiologically important roles in cell proliferation and other essential cellular processes. L‐Ara is synthesized as UDP‐L‐arabinose (UDP‐L‐Ara) from UDP‐xylose (UDP‐Xyl) by UDP‐Xyl 4‐epimerases (UXEs), a type of de novo synthesis of L‐Ara unique to plants. In Arabidopsis, the Golgi‐localized UXE AtMUR4 is the main contributor to UDP‐L‐Ara synthesis. However, cytosolic bifunctional UDP‐glucose 4‐epimerases (UGEs) with UXE activity, AtUGE1, and AtUGE3 also catalyze this reaction. For the present study, we first examined the physiological importance of bifunctional UGEs in Arabidopsis. The uge1 and uge3 mutants enhanced the dwarf phenotype of mur4 and further reduced the L‐Ara content in cell walls, suggesting that bifunctional UGEs contribute to UDP‐L‐Ara synthesis. Through the introduction of point mutations exchanging corresponding amino acid residues between AtUGE1 with high UXE activity and AtUGE2 with low UXE activity, two mutations that increase relative UXE activity of AtUGE2 were identified. The crystal structures of AtUGE2 in complex forms with NAD⁺ and NAD⁺/UDP revealed that the UDP‐binding domain of AtUGE2 has a more closed conformation and smaller sugar‐binding site than bacterial and mammalian UGEs, suggesting that plant UGEs have the appropriate size and shape for binding UDP‐Xyl and UDP‐L‐Ara to exhibit UXE activity. The presented results suggest that the capacity for cytosolic synthesis of UDP‐L‐Ara was acquired by the small sugar‐binding site and several mutations of UGEs, enabling diversified utilization of L‐Ara in seed plants.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/tpj.16779
  DOI ID：10.1111/tpj.16779, ISSN：0960-7412, eISSN：1365-313X
• Structural changes in cell wall pectic polymers contribute to freezing tolerance induced by cold acclimation in plants　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kouichi Soga; Takuma Kikuchi; Tatsuya Kutsuno; Pengfei Hao; Kazuma Sasaki; Yui Nishiyama; Satoshi Kidokoro; Arun Sampathkumar; Antony Bacic; Kim L. Johnson; Toshihisa Kotake
  Current Biology, Volume：34, Number：5, First page：958, Last page：968.e5, Mar. 2024, [Reviewed], [Last]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2024.01.045
  DOI ID：10.1016/j.cub.2024.01.045, ISSN：0960-9822
• Hydrogen chloride treatment of rice straw for upcycling into nanofibrous products for sugar pool　　　　　　　　　　　　　　　
  Ken Tokuyasu; Kenji Yamagishi; Toshihisa Kotake; Satoshi Kimura; Masakazu Ike
  Bioresource Technology Reports, Volume：25, First page：101717, Last page：101717, Feb. 2024, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.biteb.2023.101717
  DOI ID：10.1016/j.biteb.2023.101717, ISSN：2589-014X
• On the Separate Metabolisms of UDP-Sugars and GDP-Sugars in Plants
  Toshihisa Kotake; Daisuke Takahashi
  Trends in Glycoscience and Glycotechnology, Volume：36, Number：209, First page：E6, Last page：E11, Jan. 2024, [Reviewed], [Invited], [Lead, Corresponding]
  Forum: Carbohydrates Coming of Age, Scientific journal
  DOI：https://doi.org/10.4052/tigg.2303.1e
  DOI ID：10.4052/tigg.2303.1e, ISSN：0915-7352, eISSN：1883-2113
• Smooth Elongation of Pavement Cells Induced by RIC1 Overexpression Leads to Marginal Protrusions of the Cotyledon in Arabidopsis thaliana
  Kotomi Kikukawa; Hisako Takigawa-Imamura; Kouichi Soga; Toshihisa Kotake; Takumi Higaki
  Plant And Cell Physiology, Volume：64, Number：11, First page：1356, Last page：1371, Sep. 2023, [Reviewed]
  Abstract
  
  The interdigitated pavement cell shape is suggested to be mechanically rational at both the cellular and tissue levels, but the biological significance of the cell shape is not fully understood. In this study, we explored the potential importance of the jigsaw puzzle-like cell shape for cotyledon morphogenesis in Arabidopsis. We used a transgenic line overexpressing a Rho-like GTPase-interacting protein, ROP-INTERACTIVE CRIB MOTIF-CONTAINING PROTEIN 1 (RIC1), which causes simple elongation of pavement cells. Computer-assisted microscopic analyses, including virtual reality observation, revealed that RIC1 overexpression resulted in abnormal cotyledon shapes with marginal protrusions, suggesting that the abnormal organ shape might be explained by changes in the pavement cell shape. Microscopic, biochemical and mechanical observations indicated that the pavement cell deformation might be due to reduction in the cell wall cellulose content with alteration of cortical microtubule organization. To examine our hypothesis that simple elongation of pavement cells leads to an abnormal shape with marginal protrusion of the cotyledon, we developed a mathematical model that examines the impact of planar cell growth geometry on the morphogenesis of the organ that is an assemblage of the cells. Computer simulations supported experimental observations that elongated pavement cells resulted in an irregular cotyledon shape, suggesting that marginal protrusions were due to local growth variation possibly caused by stochastic bias in the direction of cell elongation cannot be explained only by polarity-based cell elongation, but that an organ-level regulatory mechanism is required.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcad094
  DOI ID：10.1093/pcp/pcad094, ISSN：0032-0781, eISSN：1471-9053
• Integrated genome-wide differentiation and association analyses identify causal genes underlying breeding-selected grain quality traits in japonica rice　　　　　　　　　　　　　　　
  Hideki Yoshida; Satoshi Okada; Fanmiao Wang; Shohei Shiota; Masaki Mori; Mayuko Kawamura; Xue Zhao; Yiqiao Wang; Naho Nishigaki; Asako Kobayashi; Kotaro Miura; Shinya Yoshida; Masaru Ikegami; Akitoshi Ito; Lin-Tzu Huang; Yue-Ie Caroline Hsing; Yoshiyuki Yamagata; Yoichi Morinaka; Masanori Yamasaki; Toshihisa Kotake; Eiji Yamamoto; Jian Sun; Ko Hirano; Makoto Matsuoka
  Molecular Plant, Volume：16, Number：9, First page：1460, Last page：1477, Sep. 2023, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.molp.2023.09.002
  DOI ID：10.1016/j.molp.2023.09.002, ISSN：1674-2052
• Autophagy accelerates cell death after desiccation and hydration stress in Physcomitrium　　　　　　　　　　　　　　　
  Kyosuke Mukae; Md. Arif Sakil; Toshihisa Kotake; Yuko Inoue-Aono; Yuji Moriyasu
  Environmental and Experimental Botany, Volume：213, First page：105412, Last page：105412, Sep. 2023, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.envexpbot.2023.105412
  DOI ID：10.1016/j.envexpbot.2023.105412, ISSN：0098-8472
• Plant type II arabinogalactan: Structural features and modification to increase functionality　　　　　　　　　　　　　　　
  Kanika Ghosh; Daisuke Takahashi; Toshihisa Kotake
  Carbohydrate Research, Volume：529, First page：108828, Last page：108828, Jul. 2023, [Reviewed], [Last, Corresponding]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2023.108828
  DOI ID：10.1016/j.carres.2023.108828, ISSN：0008-6215
• Rhamnogalacturonan-I as a nematode chemoattractant from Lotus corniculatus L. super-growing root culture
  Morihiro Oota; Syuuto Toyoda; Toshihisa Kotake; Naoki Wada; Masatsugu Hashiguchi; Ryo Akashi; Hayato Ishikawa; Bruno Favery; Allen Yi-Lun Tsai; Shinichiro Sawa
  Frontiers in Plant Science, Volume：13, First page：1008725, Jan. 2023, [Reviewed]
  Introduction
  
  The soil houses a tremendous amount of micro-organisms, many of which are plant parasites and pathogens by feeding off plant roots for sustenance. Such root pathogens and parasites often rely on plant-secreted signaling molecules in the rhizosphere as host guidance cues. Here we describe the isolation and characterization of a chemoattractant of plant-parasitic root-knot nematodes (Meloidogyne incognita, RKN).
  
  Methods
  
  The Super-growing Root (SR) culture, consisting of excised roots from the legume species Lotus corniculatus L., was found to strongly attract infective RKN juveniles and actively secrete chemoattractants into the liquid culture media. The chemo-attractant in the culture media supernatant was purified using hydrophobicity and anion exchange chromatography, and found to be enriched in carbohydrates.
  
  Results
  
  Monosaccharide analyses suggest the chemo-attractant contains a wide array of sugars, but is enriched in arabinose, galactose and galacturonic acid. This purified chemoattractant was shown to contain pectin, specifically anti-rhamnogalacturonan-I and anti-arabinogalactan protein epitopes but not anti-homogalacturonan epitopes. More importantly, the arabinose and galactose sidechain groups were found to be essential for RKN-attracting activities. This chemo-attractant appears to be specific to M. incognita, as it wasn’t effective in attracting other Meloidogyne species nor Caenorhabditis elegans.
  
  Discussion
  
  This is the first report to identify the nematode attractant purified from root exudate of L corniculatus L. Our findings re-enforce pectic carbohydrates as important chemicals mediating micro-organism chemotaxis in the soil, and also highlight the unexpected utilities of the SR culture system in root pathogen research.
  Frontiers Media SA, Scientific journal
  DOI：https://doi.org/10.3389/fpls.2022.1008725
  DOI ID：10.3389/fpls.2022.1008725, eISSN：1664-462X
• Temporal cell wall changes during cold acclimation and deacclimation and their potential involvement in freezing tolerance and growth.　　　　　　　　　　　　　　　
  Kutsuno T; Sushan Chowhan; Toshihisa Kotake; Daisuke Takahashi
  Physiologia plantarum, Volume：175, First page：e13837, Jan. 2023, [Reviewed]
  Plants adapt to freezing stress through cold acclimation, which is induced by nonfreezing low temperatures and accompanied by growth arrest. A later increase in temperature after cold acclimation leads to rapid loss of freezing tolerance and growth resumption, a process called deacclimation. Appropriate regulation of the trade-off between freezing tolerance and growth is necessary for efficient plant development in a changing environment. The cell wall, which mainly consists of polysaccharide polymers, is involved in both freezing tolerance and growth. Still, it is unclear how the balance between freezing tolerance and growth is affected during cold acclimation and deacclimation by the changes in cell wall structure and what role is played by its monosaccharide composition. Therefore, to elucidate the regulatory mechanisms controlling freezing tolerance and growth during cold acclimation and deacclimation, we investigated cell wall changes in detail by sequential fractionation and monosaccharide composition analysis in the model plant Arabidopsis thaliana, for which a plethora of information and mutant lines are available. We found that arabinogalactan proteins and pectic galactan changed in close coordination with changes in freezing tolerance and growth during cold acclimation and deacclimation. On the other hand, arabinan and xyloglucan did not return to nonacclimation levels after deacclimation but stabilized at cold acclimation levels. This indicates that deacclimation does not completely restore cell wall composition to the nonacclimated state but rather changes it to a specific novel composition that is probably a consequence of the loss of freezing tolerance and provides conditions for growth resumption.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13837
  DOI ID：10.1111/ppl.13837, ISSN：0031-9317, eISSN：1399-3054, ORCID：156844425, PubMed ID：36461890, PubMed Central ID：PMC10107845
• Surface-localized glycoproteins act through class C ARFs to fine-tune gametophore initiation in Physcomitrium patens.　　　　　　　　　　　　　　　
  Ooi-kock Teh; Singh P; Ren J; Huang LT; Ariyarathne M; Salamon BP; Wang Y; Toshihisa Kotake; Tomomichi Fujita
  Development (Cambridge, England), Volume：149, Number：24, First page：dev200370, Dec. 2022, [Reviewed]
  ABSTRACT
  
  Arabinogalactan proteins are functionally diverse cell wall structural glycoproteins that have been implicated in cell wall remodeling, although the mechanistic actions remain elusive. Here, we identify and characterize two AGP glycoproteins, SLEEPING BEAUTY (SB) and SB-like (SBL), that negatively regulate the gametophore bud initiation in Physcomitrium patens by dampening cell wall loosening/softening. Disruption of SB and SBL led to accelerated gametophore formation and altered cell wall compositions. The function of SB is glycosylation dependent and genetically connected with the class C auxin response factor (ARF) transcription factors PpARFC1B and PpARFC2. Transcriptomics profiling showed that SB upregulates PpARFC2, which in turn suppresses a range of cell wall-modifying genes that are required for cell wall loosening/softening. We further show that PpARFC2 binds directly to multiple AuxRE motifs on the cis-regulatory sequences of PECTIN METHYLESTERASE to suppress its expression. Hence, our results demonstrate a mechanism by which the SB modulates the strength of intracellular auxin signaling output, which is necessary to fine-tune the timing of gametophore initials formation.
  The Company of Biologists, Scientific journal
  DOI：https://doi.org/10.1242/dev.200370
  DOI ID：10.1242/dev.200370, ISSN：0950-1991, eISSN：1477-9129, ORCID：156844405, PubMed ID：36520083
• Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ
  Atsuko Miyagi; Kazuhisa Mori; Toshiki Ishikawa; Satoshi Ohkubo; Shunsuke Adachi; Masatoshi Yamaguchi; Taiichiro Ookawa; Toshihisa Kotake; Maki Kawai-Yamada
  Metabolomics, Volume：18, Number：12, First page：95, Nov. 2022, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s11306-022-01958-9
  DOI ID：10.1007/s11306-022-01958-9, eISSN：1573-3890, ORCID：125142203
• In vivo structural modification of type II arabinogalactans with fungal endo-β-1, 6-galactanase in Arabidopsis　　　　　　　　　　　　　　　
  Aina Kikuchi; Katsuya Hara; Yoshihisa Yoshimi; Kouichi Soga; Daisuke Takahashi; Toshihisa Kotake
  Frontiers in Plant Science, Volume：13, First page：1010492, Nov. 2022, [Reviewed], [Last, Corresponding]
  Arabinogalactan-proteins (AGPs) are mysterious extracellular glycoproteins in plants. Although AGPs are highly conserved, their molecular functions remain obscure. The physiological importance of AGPs has been extensively demonstrated with β-Yariv reagent, which specifically binds to AGPs and upon introduction into cells, causes various deleterious effects including growth inhibition and programmed cell death. However, structural features of AGPs that determine their functions have not been identified with β-Yariv reagent. It is known that AGPs are decorated with large type II arabinogalactans (AGs), which are necessary for their functions. Type II AGs consist of a β-1,3-galactan main chain and β-1,6-galactan side chains with auxiliary sugar residues such as L-arabinose and 4-O-methyl-glucuronic acid. While most side chains are short, long side chains such as β-1,6-galactohexaose (β-1,6-Gal6) also exist in type II AGs. To gain insight into the structures important for AGP functions, in vivo structural modification of β-1,6-galactan side chains was performed in Arabidopsis. We generated transgenic Arabidopsis plants expressing a fungal endo-β-1,6-galactanase, Tv6GAL, that degrades long side chains specifically under the control of dexamethasone (Dex). Two of 6 transgenic lines obtained showed more than 40 times activity of endo-β-1,6-galactanase when treated with Dex. Structural analysis indicated that long side chains such as β-1,6-Gal5 and β-1,6-Gal6 were significantly reduced compared to wild-type plants. Tv6GAL induction caused retarded growth of seedlings, which had a reduced amount of cellulose in cell walls. These results suggest that long β-1,6-galactan side chains are necessary for normal cellulose synthesis and/or deposition as their defect affects cell growth in plants.
  Frontiers Media {SA}, Scientific journal
  DOI：https://doi.org/10.3389/fpls.2022.1010492
  DOI ID：10.3389/fpls.2022.1010492, ISSN：1664-462X, ORCID：123976005
• Amino Acids Supplied through the Autophagy/Endocytosis Pathway Promote Starch Synthesis in Physcomitrella Protonemal Cells.　　　　　　　　　　　　　　　
  Md Arif Sakil; Kyosuke Mukae; Ryo Funada; Toshihisa Kotake; Shigeaki Ueno; Most Mohoshena Aktar; Md Shyduzzaman Roni; Yuko Inoue-Aono; Yuji Moriyasu
  Plants (Basel, Switzerland), Volume：11, Number：16, Aug. 2022, [Reviewed], ［International magazine］
  The physiological implications of autophagy in plant cells have not been fully elucidated. Therefore, we investigated the consequences of autophagy in the moss Physcomitrella by measuring biochemical parameters (fresh and dry weights; starch, amino acid, carbohydrate, and NH3 content) in wild-type (WT) and autophagy-deficient atg5 Physcomitrella cells. We found higher starch levels and a higher net starch synthesis rate in WT cells than in atg5 cells cultured in a glucose-containing culture medium, whereas net starch degradation was similar in the two strains cultured in a glucose-deficient culture medium. Additionally, the treatment of cells with the autophagy inhibitor 3-methyladenine suppressed starch synthesis. Loading bovine serum albumin into atg5 cells through endocytosis, i.e., supplying proteins to vacuoles in the same way as through autophagy, accelerated starch synthesis, whereas loading glutamine through the plasma membrane had no such effect, suggesting that Physcomitrella cells distinguish between different amino acid supply pathways. After net starch synthesis, NH3 levels increased in WT cells, although the change in total amino acid content did not differ between WT and atg5 cells, indicating that autophagy-produced amino acids are oxidized rapidly. We conclude that autophagy promotes starch synthesis in Physcomitrella by supplying the energy obtained by oxidizing autophagy-produced amino acids.
  English, Scientific journal
  DOI：https://doi.org/10.3390/plants11162157
  DOI ID：10.3390/plants11162157, PubMed ID：36015461, PubMed Central ID：PMC9412964
• Hydroxycinnamic acid‐modified xylan side chains and their cross‐linking products in rice cell walls are reduced in the Xylosyl arabinosyl substitution of xylan 1 mutant
  Carolina Feijao; Kris Morreel; Nadine Anders; Theodora Tryfona; Marta Busse‐Wicher; Toshihisa Kotake; Wout Boerjan; Paul Dupree
  The Plant Journal, Volume：109, Number：5, First page：1152, Last page：1167, Mar. 2022, [Reviewed]
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.15620
  DOI ID：10.1111/tpj.15620, ISSN：0960-7412, eISSN：1365-313X, ORCID：123976179
• The Mechanics and Biology of Plant Cell Walls: Resilience and Sustainability for Our Future Society　　　　　　　　　　　　　　　
  Misato Ohtani; Toshihisa Kotake; Jenny C Mortimer; Taku Demura
  Plant and Cell Physiology, Volume：62, Number：12, First page：1787, Last page：1790, Dec. 2021, [Invited], ［Domestic magazine］
  Oxford University Press (OUP), English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab168
  DOI ID：10.1093/pcp/pcab168, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：34958673
• Superoxide Production by the Red Tide-Producing Chattonella marina Complex (Raphidophyceae) Correlates with Toxicity to Aquacultured Fishes
  Tomoyuki Shikata; Koki Yuasa; Saho Kitatsuji; Setsuko Sakamoto; Kazuki Akita; Yuichiro Fujinami; Yoshitaka Nishiyama; Toshihisa Kotake; Ryusuke Tanaka; Yasuhiro Yamasaki
  Antioxidants, Volume：10, Number：10, First page：1635, Last page：1635, Oct. 2021, [Reviewed]
  The marine raphidophyte Chattonella marina complex forms red tides, causing heavy mortalities of aquacultured fishes in temperate coastal waters worldwide. The mechanism for Chattonella fish mortality remains unresolved. Although several toxic chemicals have been proposed as responsible for fish mortality, the cause is still unclear. In this study, we performed toxicity bioassays with red sea bream and yellowtail. We also measured biological parameters potentially related to ichthyotoxicity, such as cell size, superoxide (O2•−) production, and compositions of fatty acids and sugars, in up to eight Chattonella strains to investigate possible correlations with toxicity. There were significant differences in moribundity rates of fish and in all biological parameters among strains. One strain displayed no ichthyotoxicity even at high cell densities. Strains were categorized into three groups based on cell length, but this classification did not significantly correlate with ichthyotoxicity. O2•− production differed by a factor of more than 13 between strains at the late exponential growth phase. O2•− production was significantly correlated with ichthyotoxicity. Differences in fatty acid and sugar contents were not related to ichthyotoxicity. Our study supports the hypothesis that superoxide can directly or indirectly play an important role in the Chattonella-related mortality of aquacultured fishes.
  MDPI AG, Scientific journal
  DOI：https://doi.org/10.3390/antiox10101635
  DOI ID：10.3390/antiox10101635, eISSN：2076-3921
• Galactoglucomannan structure of Arabidopsis seed‐coat mucilage in GDP‐mannose synthesis impaired mutants　　　　　　　　　　　　　　　
  Naho Nishigaki; Yoshihisa Yoshimi; Hiroaki Kuki; Tadashi Kunieda; Ikuko Hara‐Nishimura; Yoichi Tsumuraya; Daisuke Takahashi; Paul Dupree; Toshihisa Kotake
  Physiologia Plantarum, Volume：173, Number：3, First page：1244, Last page：1252, Aug. 2021, [Reviewed], [Last, Corresponding], ［International magazine］
  Cell-wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases. However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear. guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides. GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis. Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan. Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan. On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure. These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13519
  DOI ID：10.1111/ppl.13519, ISSN：0031-9317, eISSN：1399-3054, PubMed ID：34380178
• Root-knot nematode chemotaxis is positively regulated by l-galactose sidechains of mucilage carbohydrate rhamnogalacturonan-I　　　　　　　　　　　　　　　
  Allen Yi-Lun Tsai; Yuka Iwamoto; Yoichi Tsumuraya; Morihiro Oota; Teruko Konishi; Shinsaku Ito; Toshihisa Kotake; Hayato Ishikawa; Shinichiro Sawa
  Science Advances, Volume：7, Number：27, First page：eabh4182, Last page：eabh4182, Jul. 2021, [Reviewed], ［International magazine］
  Root-knot nematodes (RKNs) are plant parasites and major agricultural pests. RKNs are thought to locate hosts through chemotaxis by sensing host-secreted chemoattractants; however, the structures and properties of these attractants are not well understood. Here, we describe a previously unknown RKN attractant from flaxseed mucilage that enhances infection of Arabidopsis and tomato, which resembles the pectic polysaccharide rhamnogalacturonan-I (RG-I). Fucose and galactose sidechains of the purified attractant were found to be required for attractant activity. Furthermore, the disaccharide α-l-galactosyl-1,3-l-rhamnose, which forms the linkage between the RG-I backbone and galactose sidechains of the purified attractant, was sufficient to attract RKN. These results show that the α-l-galactosyl-1,3-l-rhamnose linkage in the purified attractant from flaxseed mucilage is essential for RKN attraction. The present work also suggests that nematodes can detect environmental chemicals with high specificity, such as the presence of chiral centers and hydroxyl groups.
  American Association for the Advancement of Science (AAAS), English, Scientific journal
  DOI：https://doi.org/10.1126/sciadv.abh4182
  DOI ID：10.1126/sciadv.abh4182, eISSN：2375-2548, PubMed ID：34215589
• Wolfberry genomes and the evolution of Lycium (Solanaceae)
  You-Long Cao; Yan-long Li; Yun-Fang Fan; Zhen Li; Kouki Yoshida; Jie-Yu Wang; Xiao-Kai Ma; Ning Wang; Nobutaka Mitsuda; Toshihisa Kotake; Takeshi Ishimizu; Kun-Chan Tsai; Shan-Ce Niu; Diyang Zhang; Wei-Hong Sun; Qing Luo; Jian-Hua Zhao; Yue Yin; Bo Zhang; Jun-Yi Wang; Ken Qin; Wei An; Jun He; Guo-Li Dai; Ya-Jun Wang; Zhi-Gang Shi; En-Ning Jiao; Peng-Ju Wu; Xuedie Liu; Bin Liu; Xing-Yu Liao; Yu-Ting Jiang; Xia Yu; Yang Hao; Xin-Yu Xu; Shuang-Quan Zou; Ming-He Li; Yu-Yun Hsiao; Yu-Fu Lin; Chieh-Kai Liang; You-Yi Chen; Wan-Lin Wu; Hsiang-Chai Lu; Si-Ren Lan; Zhi-Wen Wang; Xiang Zhao; Wen-Ying Zhong; Chuan-Ming Yeh; Wen-Chieh Tsai; Yves Van de Peer; Zhong-Jian Liu
  Communications Biology, Volume：4, Number：1, Jun. 2021, [Reviewed]
  Wolfberry Lycium, an economically important genus of the Solanaceae family, contains approximately 80 species and shows a fragmented distribution pattern among the Northern and Southern Hemispheres. Although several herbaceous species of Solanaceae have been subjected to genome sequencing, thus far, no genome sequences of woody representatives have been available. Here, we sequenced the genomes of 13 perennial woody species of Lycium, with a focus on Lycium barbarum. Integration with other genomes provides clear evidence supporting a whole-genome triplication (WGT) event shared by all hitherto sequenced solanaceous plants, which occurred shortly after the divergence of Solanaceae and Convolvulaceae. We identified new gene families and gene family expansions and contractions that first appeared in Solanaceae. Based on the identification of self-incompatibility related-gene families, we inferred that hybridization hotspots are enriched for genes that might be functioning in gametophytic self-incompatibility pathways in wolfberry. Extremely low expression of LOCULE NUBER (LC) and COLORLESS NON-RIPENING (CNR) orthologous genes during Lycium fruit development and ripening processes suggests functional diversification of these two genes between Lycium and tomato. The existence of additional flowering locus C-like MADS-box genes might correlate with the perennial flowering cycle of Lycium. Differential gene expression involved in the lignin biosynthetic pathway between Lycium and tomato likely illustrates woody and herbaceous differentiation. We also provide evidence that Lycium migrated from Africa into Asia, and subsequently from Asia into North America. Our results provide functional insights into Solanaceae origins, evolution and diversification.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s42003-021-02152-8
  DOI ID：10.1038/s42003-021-02152-8, eISSN：2399-3642, ORCID：94891667
• Biochemical and structural characterization of a novel 4‐ O ‐α‐ l ‐rhamnosyl‐β‐ d ‐glucuronidase from Fusarium oxysporum
  Tatsuya Kondo; Miyu Kichijo; Makoto Nakaya; Shigeo Takenaka; Takatoshi Arakawa; Toshihisa Kotake; Shinya Fushinobu; Tatsuji Sakamoto
  The FEBS Journal, Volume：288, Number：16, First page：4918, Last page：4938, Mar. 2021, [Reviewed]
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/febs.15795
  DOI ID：10.1111/febs.15795, ISSN：1742-464X, eISSN：1742-4658
• A Pipeline towards the Biochemical Characterization of the Arabidopsis GT14 Family
  Lingling Xuan; Jie Zhang; Weitai Lu; Pawel Gluza; Berit Ebert; Toshihisa Kotake; Mengzhu Lu; Yuan Zhang; Mads H. Clausen; Kim L. Johnson; Monika S. Doblin; Joshua L. Heazlewood; Antony Bacic; Lili Song; Wei Zeng
  International Journal of Molecular Sciences, Volume：22, Number：3, First page：1360, Last page：1360, Jan. 2021, [Reviewed]
  Glycosyltransferases (GTs) catalyze the synthesis of glycosidic linkages and are essential in the biosynthesis of glycans, glycoconjugates (glycolipids and glycoproteins), and glycosides. Plant genomes generally encode many more GTs than animal genomes due to the synthesis of a cell wall and a wide variety of glycosylated secondary metabolites. The Arabidopsis thaliana genome is predicted to encode over 573 GTs that are currently classified into 42 diverse families. The biochemical functions of most of these GTs are still unknown. In this study, we updated the JBEI Arabidopsis GT clone collection by cloning an additional 105 GT cDNAs, 508 in total (89%), into Gateway-compatible vectors for downstream characterization. We further established a functional analysis pipeline using transient expression in tobacco (Nicotiana benthamiana) followed by enzymatic assays, fractionation of enzymatic products by reversed-phase HPLC (RP-HPLC) and characterization by mass spectrometry (MS). Using the GT14 family as an exemplar, we outline a strategy for identifying effective substrates of GT enzymes. By addition of UDP-GlcA as donor and the synthetic acceptors galactose-nitrobenzodiazole (Gal-NBD), β-1,6-galactotetraose (β-1,6-Gal4) and β-1,3-galactopentose (β-1,3-Gal5) to microsomes expressing individual GT14 enzymes, we verified the β-glucuronosyltransferase (GlcAT) activity of three members of this family (AtGlcAT14A, B, and E). In addition, a new family member (AT4G27480, 248) was shown to possess significantly higher activity than other GT14 enzymes. Our data indicate a likely role in arabinogalactan-protein (AGP) biosynthesis for these GT14 members. Together, the updated Arabidopsis GT clone collection and the biochemical analysis pipeline present an efficient means to identify and characterize novel GT catalytic activities.
  {MDPI} {AG}, Scientific journal
  DOI：https://doi.org/10.3390/ijms22031360
  DOI ID：10.3390/ijms22031360, eISSN：1422-0067, ORCID：87761722
• Structural features conserved in subclass of type II arabinogalactan
  Kengo Ito; Kurumi Fukuoka; Naho Nishigaki; Katsuya Hara; Yoshihisa Yoshimi; Hiroaki Kuki; Daisuke Takahashi; Yoichi Tsumuraya; Toshihisa Kotake
  Plant Biotechnology, Volume：37, Number：4, First page：459, Last page：463, Dec. 2020, [Reviewed], [Last, Corresponding]
  Japanese Society for Plant Cell and Molecular Biology, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.20.0721a
  DOI ID：10.5511/plantbiotechnology.20.0721a, ISSN：1342-4580, eISSN：1347-6114
• Unique active-site and subsite features in the arabinogalactan-degrading GH43 exo-β-1,3-galactanase from Phanerochaete chrysosporium　　　　　　　　　　　　　　　
  Kaori Matsuyama; Naomi Kishine; Zui Fujimoto; Naoki Sunagawa; Toshihisa Kotake; Yoichi Tsumuraya; Masahiro Samejima; Kiyohiko Igarashi; Satoshi Kaneko
  Journal of Biological Chemistry, Volume：295, Number：52, First page：18539, Last page：18552, Dec. 2020, [Reviewed], ［International magazine］
  Arabinogalactan proteins (AGPs) are plant proteoglycans with functions in growth and development. However, these functions are largely unexplored, mainly because of the complexity of the sugar moieties. These carbohydrate sequences are generally analyzed with the aid of glycoside hydrolases. The exo-β-1,3-galactanase is a glycoside hydrolase from the basidiomycete Phanerochaete chrysosporium (Pc1,3Gal43A), which specifically cleaves AGPs. However, its structure is not known in relation to its mechanism bypassing side chains. In this study, we solved the apo and liganded structures of Pc1,3Gal43A, which reveal a glycoside hydrolase family 43 subfamily 24 (GH43_sub24) catalytic domain together with a carbohydrate-binding module family 35 (CBM35) binding domain. GH43_sub24 is known to lack the catalytic base Asp conserved among other GH43 subfamilies. Our structure in combination with kinetic analyses reveals that the tautomerized imidic acid group of Gln263 serves as the catalytic base residue instead. Pc1,3Gal43A has three subsites that continue from the bottom of the catalytic pocket to the solvent. Subsite -1 contains a space that can accommodate the C-6 methylol of Gal, enabling the enzyme to bypass the β-1,6-linked galactan side chains of AGPs. Furthermore, the galactan-binding domain in CBM35 has a different ligand interaction mechanism from other sugar-binding CBM35s, including those that bind galactomannan. Specifically, we noted a Gly → Trp substitution, which affects pyranose stacking, and an Asp → Asn substitution in the binding pocket, which recognizes β-linked rather than α-linked Gal residues. These findings should facilitate further structural analysis of AGPs and may also be helpful in engineering designer enzymes for efficient biomass utilization.
  Elsevier BV, English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.ra120.016149
  DOI ID：10.1074/jbc.ra120.016149, ISSN：0021-9258, PubMed ID：33093171
• Calcium Binding by Arabinogalactan Polysaccharides Is Important for Normal Plant Development
  Federico Lopez-Hernandez; Theodora Tryfona; Annalisa Rizza; Xiaolan L. Yu; Matthew O.B. Harris; Alex A.R. Webb; Toshihisa Kotake; Paul Dupree
  The Plant Cell, Volume：32, Number：10, First page：3346, Last page：3369, Oct. 2020, [Reviewed]
  American Society of Plant Biologists ({ASPB}), Scientific journal
  DOI：https://doi.org/10.1105/tpc.20.00027
  DOI ID：10.1105/tpc.20.00027, ISSN：1040-4651, eISSN：1532-298X, ORCID：81355787
• Crystal structure of GH43 exo-β-1,3-galactanase from the basidiomycetePhanerochaete chrysosporium provides insights into the mechanism of bypassing side chains　　　　　　　　　　　　　　　
  Kaori Matsuyama; Naomi Kishine; Zui Fujimoto; Naoki Sunagawa; Toshihisa Kotake; Yoichi Tsumuraya; Masahiro Samejima; Kiyohiko Igarashi; Satoshi Kaneko
  Sep. 2020, [Reviewed]
  DOI：https://doi.org/10.1101/2020.09.23.310037
  DOI ID：10.1101/2020.09.23.310037, ORCID：80848695
• Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape　　　　　　　　　　　　　　　
  Yoshihisa Yoshimi; Katsuya Hara; Mami Yoshimura; Nobukazu Tanaka; Takumi Higaki; Yoichi Tsumuraya; Toshihisa Kotake
  Journal of Experimental Botany, Volume：71, Number：18, First page：5414, Last page：5424, Sep. 2020, [Reviewed], [Last, Corresponding], ［International magazine］
  Arabinogalactan-proteins (AGPs) are a family of plant extracellular proteoglycans implicated in many physiological events. AGP is decorated with type II arabinogalactans (AGs) consisting of a β-1,3-galactan backbone and β-1,6-galactan side chains, to which other sugars are attached. Based on the fact that a type II AG-specific inhibitor, β-Yariv reagent, perturbs growth and development, it has been proposed that type II AGs participate in the regulation of cell shape and tissue organization. However, the mechanisms by which type II AGs participate have not yet been established. Here, we describe a novel system that causes specific degradation of type II AGs in Arabidopsis, by which a gene encoding a fungal exo-β-1,3-galactanase that specifically hydrolyzes β-1,3-galactan backbones of type II AGs is expressed under the control of a dexamethasone-inducible promoter. Dexamethasone treatment increased the galactanase activity, leading to a decrease in Yariv reagent-reactive AGPs in transgenic Arabidopsis. We detected the typical oligosaccharides released from type II AGs by Il3GAL in the soluble fraction, demonstrating that Il3GAL acted on type II AG in the transgenic plants. Additionally, this resulted in severe tissue disorganization in the hypocotyl and cotyledons, suggesting that the degradation of type II AGs affected the regulation of cell shape.
  Oxford University Press (OUP), English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/eraa236
  DOI ID：10.1093/jxb/eraa236, ISSN：0022-0957, eISSN：1460-2431, PubMed ID：32470141, PubMed Central ID：PMC7501824
• Microgravity affects the level of matrix polysaccharide 1,3:1,4-beta-glucans in cell walls of rice shoots by increasing the expression level of a gene involved in their breakdown　　　　　　　　　　　　　　　
  Wakabayashi K; Soga K; Hoson T; Kotake T; Yamazaki T; Ishioka N; Shimazu T; Kamada M
  Astrobiology, Volume：20, First page：820, Last page：829, 2020, [Reviewed]
  English
• Properties of arabinogalactan-proteins in European pear (Pyrus communis L.) fruits
  Tsumuraya, Y.; Ozeki, E.; Ooki, Y.; Yoshimi, Y.; Hashizume, K.; Kotake, T.
  Carbohydrate Research, Volume：485, First page：107816, Last page：107816, Nov. 2019, [Reviewed], [Last, Corresponding]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2019.107816
  DOI ID：10.1016/j.carres.2019.107816, ISSN：0008-6215, ORCID：63916521, SCOPUS ID：85072301772
• Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum
  Fujita, K.; Sakamoto, A.; Kaneko, S.; Kotake, T.; Tsumuraya, Y.; Kitahara, K.
  Applied Microbiology and Biotechnology, Volume：103, Number：3, First page：1299, Last page：1310, Feb. 2019, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s00253-018-9566-4
  DOI ID：10.1007/s00253-018-9566-4, ISSN：0175-7598, eISSN：1432-0614, ORCID：63916500, SCOPUS ID：85061872997
• Yariv reactivity of type II arabinogalactan from larch wood　　　　　　　　　　　　　　　
  Sato, K.; Hara, K.; Yoshimi, Y.; Kitazawa, K.; Ito, H.; Tsumuraya, Y.; Kotake, T.
  Carbohydrate Research, Volume：467, First page：8, Last page：13, Sep. 2018, [Reviewed], [Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2018.07.004
  DOI ID：10.1016/j.carres.2018.07.004, ISSN：0008-6215, ORCID：63916540, PubMed ID：30036728, SCOPUS ID：85050163032
• The patterned structure of galactoglucomannan suggests it may bind to cellulose in seed mucilage　　　　　　　　　　　　　　　
  Yu, L.; Lyczakowski, J.J.; Pereira, C.S.; Kotake, T.; Yu, X.; Li, A.; Mogelsvang, S.; Skaf, M.S.; Dupree, P.
  Plant Physiology, Volume：178, Number：3, Sep. 2018, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1104/pp.18.00709
  DOI ID：10.1104/pp.18.00709, ISSN：0032-0889, ORCID：63916531, PubMed ID：30185440, SCOPUS ID：85055514451
• Correction to: Metabolism of l-arabinose in plants (Journal of Plant Research, (2016), 129, 5, (781-792), 10.1007/s10265-016-0834-z)
  Kotake, T.; Yamanashi, Y.; Imaizumi, C.; Tsumuraya, Y.
  Journal of Plant Research, Volume：131, Number：3, First page：565, Last page：565, 2018
  Scientific journal
  DOI：https://doi.org/10.1007/s10265-018-1012-2
  DOI ID：10.1007/s10265-018-1012-2, ORCID：63916543, SCOPUS ID：85042225730
• Modification of growth anisotropy and cortical microtubule dynamics in Arabidopsis hypocotyls grown under microgravity conditions in space　　　　　　　　　　　　　　　
  Kouichi Soga; Chiaki Yamazaki; Motoshi Kamada; Naoki Tanigawa; Haruo Kasahara; Sachiko Yano; Kei H. Kojo; Natsumaro Kutsuna; Takehide Kato; Takashi Hashimoto; Toshihisa Kotake; Kazuyuki Wakabayashi; Takayuki Hoson
  Physiologia Plantarum, Volume：162, Number：1, First page：135, Last page：144, Jan. 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.12640
  DOI ID：10.1111/ppl.12640, ISSN：0031-9317, eISSN：1399-3054, PubMed ID：28862767, Web of Science ID：WOS:000418236000009
• Properties of two fungal endo-beta-1,3-galactanases and their synergistic action with an exo-beta-1,3-galactanase in degrading arabinogalactan-proteins　　　　　　　　　　　　　　　
  Yoshihisa Yoshimi; Kaori Yaguchi; Satoshi Kaneko; Yoichi Tsumuraya; Toshihisa Kotake
  Carbohydrate Research, Volume：453-454, First page：26, Last page：35, Dec. 2017, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2017.10.013
  DOI ID：10.1016/j.carres.2017.10.013, ISSN：0008-6215, eISSN：1873-426X, PubMed ID：29121496, Web of Science ID：WOS:000416212600004
• A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies　　　　　　　　　　　　　　　
  Colin Ruprecht; Max P. Bartetzko; Deborah Senf; Pietro Dallabernadina; Irene Boos; Mathias C. F. Andersen; Toshihisa Kotake; J. Paul Knox; Michael G. Hahn; Mads H. Clausen; Fabian Pfrengle
  Plant Physiology, Volume：175, Number：3, First page：1094, Last page：1104, Nov. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.17.00737
  DOI ID：10.1104/pp.17.00737, ISSN：0032-0889, eISSN：1532-2548, PubMed ID：28924016, Web of Science ID：WOS:000414052500007
• Persistence of plant hormone levels in rice shoots grown under microgravity conditions in space: its relationship to maintenance of shoot growth　　　　　　　　　　　　　　　
  Kazuyuki Wakabayashi; Kouichi Soga; Takayuki Hoson; Toshihisa Kotake; Mikiko Kojima; Hitoshi Sakakibara; Takashi Yamazaki; Akira Higashibata; Noriaki Ishioka; Toru Shimazu; Motoshi Kamada
  Physiologia Plantarum, Volume：161, Number：2, First page：285, Last page：293, Oct. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.12591
  DOI ID：10.1111/ppl.12591, ISSN：0031-9317, eISSN：1399-3054, PubMed ID：28573759, Web of Science ID：WOS:000417402000008
• Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1　　　　　　　　　　　　　　　
  Ko Hirano; Reiko Masuda; Wakana Takase; Yoichi Morinaka; Mayuko Kawamura; Yoshinobu Takeuchi; Hiroki Takagi; Hiroki Yaegashi; Satoshi Natsume; Ryohei Terauchi; Toshihisa Kotake; Yasuyuki Matsushita; Takashi Sazuka
  Planta, Volume：246, Number：1, First page：61, Last page：74, Jul. 2017, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1007/s00425-017-2685-9
  DOI ID：10.1007/s00425-017-2685-9, ISSN：0032-0935, eISSN：1432-2048, PubMed ID：28357539, Web of Science ID：WOS:000404150900005
• Heterologous expression and characterization of an Arabidopsis β-L-arabinopyranosidase and α-D-galactosidases acting on β-L-arabinopyranosyl residues.　　　　　　　　　　　　　　　
  Chiemi Imaizumi; Harumi Tomatsu; Kiminari Kitazawa; Yoshihisa Yoshimi; Seiji Shibano; Kaoru Kikuchi; Masatoshi Yamaguchi; Satoshi Kaneko; Yoichi Tsumuraya; Toshihisa Kotake
  Journal of Experimental Botany, Volume：68, Number：16, First page：4651, Last page：4661, Jul. 2017, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erx279
  DOI ID：10.1093/jxb/erx279, ISSN：0022-0957, eISSN：1460-2431, PubMed ID：28981776, Web of Science ID：WOS:000412100500020
• A protease/peptidase from culture medium of Flammulina velutipes that acts on arabinogalactan-protein　　　　　　　　　　　　　　　
  Yoshihisa Yoshimi; Yumi Sugawara; Chiaki Hori; Kiyohiko Igarashi; Satoshi Kaneko; Yoichi Tsumuraya; Toshihisa Kotake
  Bioscience, Biotechnology and Biochemistry, Volume：81, Number：3, First page：475, Last page：481, Mar. 2017, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1080/09168451.2016.1258985
  DOI ID：10.1080/09168451.2016.1258985, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：27884087, Web of Science ID：WOS:000395102900010
• Structure and functions of arabinogalactan-proteins, a family of plant proteoglycans
  Tsumuraya, Y.; Kotake, T.
  Seikagaku, Volume：89, Number：4, 2017
  Scientific journal
  DOI：https://doi.org/10.14952/SEIKAGAKU.2017.890498
  DOI ID：10.14952/SEIKAGAKU.2017.890498, ORCID：63916496, SCOPUS ID：85042885786
• Metabolism of L-arabinose in plants　　　　　　　　　　　　　　　
  Toshihisa Kotake; Yukiko Yamanashi; Chiemi Imaizumi; Yoichi Tsumuraya
  Journal of Plant Research, Volume：129, Number：5, First page：781, Last page：792, Sep. 2016, [Reviewed], [Invited], [Lead, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-016-0834-z
  DOI ID：10.1007/s10265-016-0834-z, ISSN：0918-9440, eISSN：1618-0860, PubMed ID：27220955, Web of Science ID：WOS:000381379700001
• Roles of MAP65-1 and BPP1 in gravity resistance of Arabidopsis hypocotyls　　　　　　　　　　　　　　　
  Murakami M; Soga K; Kotake T; Kato T; Hashimoto T; Wakabayashi K; Hoson T
  Biological Sciences in Space, Volume：30, First page：1, Last page：7, Aug. 2016, [Reviewed]
  English
  DOI：https://doi.org/10.2187/bss.30.1
  DOI ID：10.2187/bss.30.1, ISSN：0914-9201, CiNii Articles ID：120006369769, CiNii Books ID：AN10164806
• Precise estimation of genomic regions controlling lodging resistance using a set of reciprocal chromosome segment substitution lines in rice　　　　　　　　　　　　　　　
  Taiichiro Ookawa; Ryo Aoba; Toshio Yamamoto; Tadamasa Ueda; Toshiyuki Takai; Shuichi Fukuoka; Tsuyu Ando; Shunsuke Adachi; Makoto Matsuoka; Takeshi Ebitani; Yoichiro Kato; Indria Wahyu Mulsanti; Masahiro Kishii; Matthew Reynolds; Francisco Pinera; Toshihisa Kotake; Shinji Kawasaki; Takashi Motobayashi; Tadashi Hirasawa
  Scientific Reports, Volume：6, First page：30572, Jul. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep30572
  DOI ID：10.1038/srep30572, ISSN：2045-2322, PubMed ID：27465821, Web of Science ID：WOS:000380975700001
• The AMOR Arabinogalactan Sugar Chain Induces Pollen-Tube Competency to Respond to Ovular Guidance.　　　　　　　　　　　　　　　
  Akane G Mizukami; Rie Inatsugi; Jiao Jiao; Toshihisa Kotake; Keiko Kuwata; Kento Ootani; Satohiro Okuda; Subramanian Sankaranarayanan; Yoshikatsu Sato; Daisuke Maruyama; Hiroaki Iwai; Estelle Garénaux; Chihiro Sato; Ken Kitajima; Yoichi Tsumuraya; Hitoshi Mori; Junichiro Yamaguchi; Kenichiro Itami; Narie Sasaki; Tetsuya Higashiyama
  Current Biology : CB, Volume：26, Number：8, First page：1091, Last page：7, Apr. 2016, [Reviewed], ［International magazine］
  Precise directional control of pollen-tube growth by pistil tissue is critical for successful fertilization of flowering plants [1-3]. Ovular attractant peptides, which are secreted from two synergid cells on the side of the egg cell, have been identified [4-6]. Emerging evidence suggests that the ovular directional cue is not sufficient for successful guidance but that competency control by the pistil is critical for the response of pollen tubes to the attraction signal [1, 3, 7]. However, the female molecule for this competency induction has not been reported. Here we report that ovular methyl-glucuronosyl arabinogalactan (AMOR) induces competency of the pollen tube to respond to ovular attractant LURE peptides in Torenia fournieri. We developed a method for assaying the response capability of a pollen tube by micromanipulating an ovule. Using this method, we showed that pollen tubes growing through a cut style acquired a response capability in the medium by receiving a sufficient amount of a factor derived from mature ovules of Torenia. This factor, named AMOR, was identified as an arabinogalactan polysaccharide, the terminal 4-O-methyl-glucuronosyl residue of which was necessary for its activity. Moreover, a chemically synthesized disaccharide, the β isomer of methyl-glucuronosyl galactose (4-Me-GlcA-β-(1→6)-Gal), showed AMOR activity. No specific sugar-chain structure of plant extracellular matrix has been identified as a bioactive molecule involved in intercellular communication. We suggest that the AMOR sugar chain in the ovary renders the pollen tube competent to the chemotropic response prior to final guidance by LURE peptides.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2016.02.040
  DOI ID：10.1016/j.cub.2016.02.040, ISSN：0960-9822, PubMed ID：27068416
• KONJAC1 and 2 Are Key Factors for GDP-Mannose Generation and Affect L-Ascorbic Acid and Glucomannan Biosynthesis in Arabidopsis　　　　　　　　　　　　　　　
  Shota Sawake; Noriaki Tajima; Jenny C. Mortimer; Jeemeng Lao; Toshiki Ishikawa; Xiaolan Yu; Yukiko Yamanashi; Yoshihisa Yoshimi; Maki Kawai-Yamada; Paul Dupree; Yoichi Tsumuraya; Toshihisa Kotake
  Plant Cell, Volume：27, Number：12, First page：3397, Last page：3409, Dec. 2015, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.15.00379
  DOI ID：10.1105/tpc.15.00379, ISSN：1040-4651, eISSN：1532-298X, PubMed ID：26672069, Web of Science ID：WOS:000368297100010
• l-Fucose-containing arabinogalactan-protein in radish leaves　　　　　　　　　　　　　　　
  Miho Inaba; Takuma Maruyama; Yoshihisa Yoshimi; Toshihisa Kotake; Koji Matsuoka; Tetsuo Koyama; Theodora Tryfona; Paul Dupree; Yoichi Tsumuraya
  Carbohydrate Research, Volume：415, First page：1, Last page：11, Oct. 2015, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2015.07.002
  DOI ID：10.1016/j.carres.2015.07.002, ISSN：0008-6215, eISSN：1873-426X, PubMed ID：26267887, Web of Science ID：WOS:000362889100001
• Suppression of Hydroxycinnamate Network Formation in Cell Walls of Rice Shoots Grown under Microgravity Conditions in Space　　　　　　　　　　　　　　　
  Kazuyuki Wakabayashi; Kouichi Soga; Takayuki Hoson; Toshihisa Kotake; Takashi Yamazaki; Akira Higashibata; Noriaki Ishioka; Toru Shimazu; Keiji Fukui; Ikuko Osada; Haruo Kasahara; Motoshi Kamada
  PLoS One, Volume：10, Number：9, First page：e0137992, Sep. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0137992
  DOI ID：10.1371/journal.pone.0137992, ISSN：1932-6203, PubMed ID：26378793, Web of Science ID：WOS:000361769400042
• Hormonal regulation of gummosis and composition of gums from bulbs of hyacinth (Hyacinthus orientalis)　　　　　　　　　　　　　　　
  Kensuke Miyamoto; Toshihisa Kotake; Anna Jarecka Boncela; Marian Saniewski; Junichi Ueda
  Journal of Plant Physiology, Volume：174, First page：1, Last page：4, Feb. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2014.10.007
  DOI ID：10.1016/j.jplph.2014.10.007, ISSN：0176-1617, eISSN：1618-1328, PubMed ID：25462960, Web of Science ID：WOS:000346488700001
• Action of an endo-beta-1,3(4)-glucanase on cellobiosyl unit structure in barley beta-1,3:1,4-glucan　　　　　　　　　　　　　　　
  Takao Kuge; Hiroki Nagoya; Theodora Tryfona; Tsunemi Kurokawa; Yoshihisa Yoshimi; Naoshi Dohmae; Kazufumi Tsubaki; Paul Dupree; Yoichi Tsumuraya; Toshihisa Kotake
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：79, Number：11, First page：1810, Last page：1817, 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1080/09168451.2015.1046365
  DOI ID：10.1080/09168451.2015.1046365, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：26027730, Web of Science ID：WOS:000371305900009
• Enzymatic activity and substrate specificity of the recombinant tomato beta-galactosidase 1　　　　　　　　　　　　　　　
  Masahiro Eda; Megumi Ishimaru; Toshiji Tada; Tatsuji Sakamoto; Toshihisa Kotake; Yoichi Tsumuraya; Andrew J. Mort; Kenneth C. Gross
  Journal of Plant Physiology, Volume：171, Number：16, First page：1454, Last page：1460, Oct. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2014.06.010
  DOI ID：10.1016/j.jplph.2014.06.010, ISSN：0176-1617, eISSN：1618-1328, PubMed ID：25105231, Web of Science ID：WOS:000345632200001
• Enzymatic fragmentation of carbohydrate moieties of radish arabinogalactan-protein and elucidation of the structures　　　　　　　　　　　　　　　
  Ryohei Shimoda; Kohei Okabe; Toshihisa Kotake; Koji Matsuoka; Tetsuo Koyama; Theodora Tryfona; Hui-Chung Liang; Paul Dupree; Yoichi Tsumuraya
  Bioscience, Biotechnology, and Biochemistry, Volume：78, Number：5, First page：818, Last page：831, May 2014, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1080/09168451.2014.910100
  DOI ID：10.1080/09168451.2014.910100, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：25035985, Web of Science ID：WOS:000339070600015
• Characterization of alkali-soluble polysaccharides in deep subsoil layers　　　　　　　　　　　　　　　
  Toshihisa Kotake; Shoichiro Hamamoto; Takeshi Saito; Junichi Ohnishi; Toshiko Komatsu; Yoichi Tsumuraya
  Soil Science and Plant Nutrition, Volume：59, Number：6, First page：871, Last page：876, Dec. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1080/00380768.2013.853280
  DOI ID：10.1080/00380768.2013.853280, ISSN：0038-0768, eISSN：1747-0765, Web of Science ID：WOS:000328245100005
• Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-bound beta-glucuronosyltransferases from radish primary roots　　　　　　　　　　　　　　　
  Maya Endo; Toshihisa Kotake; Yoko Watanabe; Kazumasa Kimura; Yoichi Tsumuraya
  PLANTA, Volume：238, Number：6, First page：1157, Last page：1169, Dec. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-013-1959-0
  DOI ID：10.1007/s00425-013-1959-0, ISSN：0032-0935, eISSN：1432-2048, PubMed ID：24057431, Web of Science ID：WOS:000327386200012
• A beta-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth　　　　　　　　　　　　　　　
  Eva Knoch; Adiphol Dilokpimol; Theodora Tryfona; Christian P. Poulsen; Guangyan Xiong; Jesper Harholt; Bent L. Petersen; Peter Ulvskov; Masood Z. Hadi; Toshihisa Kotake; Yoichi Tsumuraya; Markus Pauly; Paul Dupree; Naomi Geshi
  Plant Journal, Volume：76, Number：6, First page：1016, Last page：1029, Dec. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.12353
  DOI ID：10.1111/tpj.12353, ISSN：0960-7412, eISSN：1365-313X, PubMed ID：24128328, Web of Science ID：WOS:000328014300009
• A galactosyltransferase acting on arabinogalactan protein glycans is essential for embryo development in Arabidopsis　　　　　　　　　　　　　　　
  Naomi Geshi; Jorunn N. Johansen; Adiphol Dilokpimol; Aurélia Rolland; Katia Belcram; Stéphane Verger; Toshihisa Kotake; Yoichi Tsumuraya; Satoshi Kaneko; Theodora Tryfona; Paul Dupree; Henrik V. Scheller; Herman Höfte; Gregory Mouille
  Plant Journal, Volume：76, Number：1, First page：128, Last page：137, Oct. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.12281
  DOI ID：10.1111/tpj.12281, ISSN：0960-7412, PubMed ID：23837821, SCOPUS ID：84884903407
• beta-Galactosyl Yariv Reagent Binds to the beta-1,3-Galactan of Arabinogalactan Proteins　　　　　　　　　　　　　　　
  Kiminari Kitazawa; Theodora Tryfona; Yoshihisa Yoshimi; Yoshihiro Hayashi; Susumu Kawauchi; Liudmil Antonov; Hiroshi Tanaka; Takashi Takahashi; Satoshi Kaneko; Paul Dupree; Yoichi Tsumuraya; Toshihisa Kotake
  Plant Physiology, Volume：161, Number：3, First page：1117, Last page：1126, Mar. 2013, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.112.211722
  DOI ID：10.1104/pp.112.211722, ISSN：0032-0889, PubMed ID：23296690, Web of Science ID：WOS:000316984100007
• Structural Characterization of Arabidopsis Leaf Arabinogalactan Polysaccharides　　　　　　　　　　　　　　　
  Theodora Tryfona; Hui-Chung Liang; Toshihisa Kotake; Yoichi Tsumuraya; Elaine Stephens; Paul Dupree
  Plant Physiology, Volume：160, Number：2, First page：653, Last page：666, Oct. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.112.202309
  DOI ID：10.1104/pp.112.202309, ISSN：0032-0889, PubMed ID：22891237, Web of Science ID：WOS:000309523800008
• The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp strain HK-01 in NaCl tolerance　　　　　　　　　　　　　　　
  Hidehisa Yoshimura; Toshihisa Kotake; Tsutomu Aohara; Yoichi Tsumuraya; Masahiko Ikeuchi; Masayuki Ohmori
  Journal of Applied Phycology, Volume：24, Number：2, First page：237, Last page：243, Apr. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10811-011-9672-5
  DOI ID：10.1007/s10811-011-9672-5, ISSN：0921-8971, Web of Science ID：WOS:000300888200010
• Structural and Biochemical Characterization of Glycoside Hydrolase Family 79 beta-Glucuronidase from Acidobacterium capsulatum　　　　　　　　　　　　　　　
  Mari Michikawa; Hitomi Ichinose; Mitsuru Momma; Peter Biely; Seino Jongkees; Makoto Yoshida; Toshihisa Kotake; Yoichi Tsumuraya; Stephen G. Withers; Zui Fujimoto; Satoshi Kaneko
  Journal of Biological Chemistry, Volume：287, Number：17, First page：14069, Last page：14077, Apr. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.M112.346288
  DOI ID：10.1074/jbc.M112.346288, ISSN：0021-9258, PubMed ID：22367201, Web of Science ID：WOS:000303996300060
• Changes in the transcript levels of microtubule-associated protein MAP65-1 during reorientation of cortical microtubules in azuki bean epicotyls　　　　　　　　　　　　　　　
  Soga, Kouichi; Kotake; Toshihisa; Wakabayashi, Kazuyuki; Hoson, Takayuki
  Acta Physiologiae Plantarum, Volume：34, Number：2, First page：533, Last page：540, Mar. 2012, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1007/s11738-011-0850-5
  DOI ID：10.1007/s11738-011-0850-5, ISSN：0137-5881, ORCID：20757413, Web of Science ID：WOS:000300453500014
• Endo-beta-1,3-galactanase from Winter Mushroom Flammulina velutipes　　　　　　　　　　　　　　　
  Toshihisa Kotake; Naohiro Hirata; Yuta Degi; Maki Ishiguro; Kiminari Kitazawa; Ryohei Takata; Hitomi Ichinose; Satoshi Kaneko; Kiyohiko Igarashi; Masahiro Samejima; Yoichi Tsumuraya
  Journal of Biological Chemistry, Volume：286, Number：31, First page：27848, Last page：27854, Aug. 2011, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.M111.251736
  DOI ID：10.1074/jbc.M111.251736, ISSN：0021-9258, PubMed ID：21653698, Web of Science ID：WOS:000293268700076
• Rice Brittle culm 6 encodes a dominant-negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls　　　　　　　　　　　　　　　
  Toshihisa Kotake; Tsutomu Aohara; Ko Hirano; Ami Sato; Yasuko Kaneko; Yoichi Tsumuraya; Hiroshi Takatsuji; Shinji Kawasaki
  Journal of Experimental Botany, Volume：62, Number：6, First page：2053, Last page：2062, Mar. 2011, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erq395
  DOI ID：10.1093/jxb/erq395, ISSN：0022-0957, PubMed ID：21209026, Web of Science ID：WOS:000288553000026
• Carbohydrate structural analysis of wheat flour arabinogalactan protein　　　　　　　　　　　　　　　
  Theodora Tryfona; Hui-Chung Liang; Toshihisa Kotake; Satoshi Kaneko; Justin Marsh; Hitomi Ichinose; Alison Lovegrove; Yoichi Tsumuraya; Peter R. Shewry; Elaine Stephens; Paul Dupree
  Carbohydrate Research, Volume：345, Number：18, First page：2648, Last page：2656, Dec. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2010.09.018
  DOI ID：10.1016/j.carres.2010.09.018, ISSN：0008-6215, PubMed ID：20971454, Web of Science ID：WOS:000285657700010
• Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa　　　　　　　　　　　　　　　
  Ryohei Takata; Keita Tokita; Satoko Mori; Ryohei Shimoda; Naoki Harada; Hitomi Ichinose; Satoshi Kaneko; Kiyohiko Igarashi; Masahiro Samejima; Yoichi Tsumuraya; Toshihisa Kotake
  Carbohydrate Research, Volume：345, Number：17, First page：2516, Last page：2522, Nov. 2010, [Reviewed], [Last, Corresponding]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2010.09.006
  DOI ID：10.1016/j.carres.2010.09.006, ISSN：0008-6215
• Chemoenzymatic Synthesis, Inhibition Studies, and X-ray Crystallographic Analysis of the Phosphono Analog of UDP-Galp as an Inhibitor and Mechanistic Probe for UDP-Galactopyranose Mutase　　　　　　　　　　　　　　　
  Sarathy Karunan Partha; Ali Sadeghi-Khomami; Kathryn Slowski; Toshihisa Kotake; Neil R. Thomas; David L. Jakeman; David A. R. Sanders
  Journal of Molecular Biology, Volume：403, Number：4, First page：578, Last page：590, Nov. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jmb.2010.08.053
  DOI ID：10.1016/j.jmb.2010.08.053, ISSN：0022-2836, PubMed ID：20850454, Web of Science ID：WOS:000284440500007
• Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa　　　　　　　　　　　　　　　
  Ryohei Takata; Keita Tokita; Satoko Mori; Ryohei Shimoda; Naoki Harada; Hitomi Ichinose; Satoshi Kaneko; Kiyohiko Igarashi; Masahiro Samejima; Yoichi Tsumuraya; Toshihisa Kotake
  Carbohydrate Research, Volume：345, Number：17, First page：2516, Last page：2522, Nov. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2010.09.006
  DOI ID：10.1016/j.carres.2010.09.006, ISSN：0008-6215, PubMed ID：20932514, Web of Science ID：WOS:000284520500013
• Rice BRITTLE CULM 3 (BC3) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis　　　　　　　　　　　　　　　
  Ko Hirano; Toshihisa Kotake; Kumiko Kamihara; Kahori Tsuna; Tsutomu Aohara; Yasuko Kaneko; Hiroshi Takatsuji; Yoichi Tsumuraya; Shinji Kawasaki
  Planta, Volume：232, Number：1, First page：95, Last page：108, Jun. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-010-1145-6
  DOI ID：10.1007/s00425-010-1145-6, ISSN：0032-0935, PubMed ID：20369251, Web of Science ID：WOS:000277792700008
• Generation of nucleotide sugars for biomass formation in plants　　　　　　　　　　　　　　　
  Toshihisa Kotake; Chie Hirosawa; Yasutoshi Ando; Yoichi Tsumuraya
  Plant Biotechnology, Volume：27, Number：3, First page：231, Last page：236, 2010, [Reviewed], [Invited], [Lead, Corresponding]
  English, Scientific journal
  ISSN：1342-4580, Web of Science ID：WOS:000280085800003
• Sugar treatment inhibits IAA-induced expression of endo-1,3:1,4-beta-glucanase EI transcripts in barley coleoptile segments　　　　　　　　　　　　　　　
  Hiroyuki Takeda; Tomoko Sugahara; Toshihisa Kotake; Naoki Nakagawa; Naoki Sakurai
  Physiologia Plantarum, Volume：139, Number：4, First page：413, Last page：420, 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1399-3054.2010.01372.x
  DOI ID：10.1111/j.1399-3054.2010.01372.x, ISSN：0031-9317, PubMed ID：20412461, Web of Science ID：WOS:000279840300007
• 1-Aminocyclopropane-1-carboxylic acid (ACC)-induced reorientation of cortical microtubules is accompanied by a transient increase in the transcript levels of gamma-tubulin complex and katanin genes in azuki bean epicotyls　　　　　　　　　　　　　　　
  Kouichi Soga; Aya Yamaguchi; Toshihisa Kotake; Kazuyuki Wakabayashi; Takayuki Hoson
  Journal of Plant Physiology, Volume：167, Number：14, First page：1165, Last page：1171, 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2010.04.001
  DOI ID：10.1016/j.jplph.2010.04.001, ISSN：0176-1617, PubMed ID：20451287, Web of Science ID：WOS:000281886500007
• Transient increase in the levels of γ-tubulin complex and katanin are responsible for reorientation by ethylene and hypergravity of cortical microtubules　　　　　　　　　　　　　　　
  Kouichi Soga; Aya Yamaguchi; Toshihisa Kotake; Kazuyuki Wakabayashi; Takayuki Hoson
  Plant Signaling and Behavior, Volume：5, Number：11, First page：1480, Last page：1482, 2010, [Reviewed]
  Landes Bioscience, English, Scientific journal
  DOI：https://doi.org/10.4161/psb.5.11.13561
  DOI ID：10.4161/psb.5.11.13561, ISSN：1559-2324, PubMed ID：21051953, SCOPUS ID：85046916459
• Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants　　　　　　　　　　　　　　　
  Kotake, Toshihisa; Takata, Ryohei; Verma, Rajeev; Takaba, Masato; Yamaguchi, Daisuke; Orita, Takahiro; Kaneko, Satoshi; Matsuoka, Koji; Koyama, Tetsuo; Reiter, Wolf-Dieter; Tsumuraya, Yoichi
  Biochemical Journal, Volume：424, Number：2, First page：169, Last page：177, Dec. 2009, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1042/BJ20091025
  DOI ID：10.1042/BJ20091025, ISSN：0264-6021, eISSN：1470-8728, ORCID：20757452, Web of Science ID：WOS:000272135100002
• Gummosis in grape hyacinth (Muscari armeniacum) bulbs: hormonal regulation and chemical composition of gums
  Kensuke Miyamoto; Toshihisa Kotake; Makiko Sasamoto; Marian Saniewski; Junichi Ueda
  Journal of Plant Research, Volume：123, Number：3, First page：363, Last page：370, Nov. 2009, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s10265-009-0273-1
  DOI ID：10.1007/s10265-009-0273-1, ISSN：0918-9440, eISSN：1618-0860
• Rice BRITTLE CULM 5 (BRITTLE NODE) is Involved in Secondary Cell Wall Formation in the Sclerenchyma Tissue of Nodes
  Tsutomu Aohara; Toshihisa Kotake; Yasuko Kaneko; Hiroshi Takatsuji; Yoichi Tsumuraya; Shinji Kawasaki
  Plant and Cell Physiology, Volume：50, Number：11, First page：1886, Last page：1897, Nov. 2009, [Reviewed]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcp133
  DOI ID：10.1093/pcp/pcp133, ISSN：0032-0781, eISSN：1471-9053
• The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots
  Rumi Tominaga‐Wada; Mineko Iwata; Junji Sugiyama; Toshihisa Kotake; Tetsuya Ishida; Ryusuke Yokoyama; Kazuhiko Nishitani; Kiyotaka Okada; Takuji Wada
  The Plant Journal, Volume：60, Number：3, First page：564, Last page：574, Oct. 2009, [Reviewed]
  Summary
  
  Arabidopsis root hair formation is determined by the patterning genes CAPRICE (CPC), GLABRA3 (GL3), WEREWOLF (WER) and GLABRA2 (GL2), but little is known about the later changes in cell wall material during root hair formation. A combined Fourier‐transform infrared microspectroscopy–principal components analysis (FTIR‐PCA) method was used to detect subtle differences in the cell wall material between wild‐type and root hair mutants in Arabidopsis. Among several root hair mutants, only the gl2 mutation affected root cell wall polysaccharides. Five of the 10 genes encoding cellulose synthase (CESA1–10) and 4 of 33 xyloglucan endotransglucosylase (XTH1–33) genes in Arabidopsis are expressed in the root, but only CESA5 and XTH17 were affected by the gl2 mutation. The L1‐box sequence located in the promoter region of these genes was recognized by the GL2 protein. These results indicate that GL2 directly regulates cell wall‐related gene expression during root development.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313x.2009.03976.x
  DOI ID：10.1111/j.1365-313x.2009.03976.x, ISSN：0960-7412, eISSN：1365-313X
• Molecular Cloning and Expression inPichia pastorisof aIrpex lacteusExo-β-(1→3)-galactanase Gene
  Toshihisa KOTAKE; Kiminari KITAZAWA; Ryohei TAKATA; Kohei OKABE; Hitomi ICHINOSE; Satoshi KANEKO; Yoichi TSUMURAYA
  Bioscience, Biotechnology, and Biochemistry, Volume：73, Number：10, First page：2303, Last page：2309, Oct. 2009, [Reviewed], [Lead]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1271/bbb.90433
  DOI ID：10.1271/bbb.90433, ISSN：0916-8451, eISSN：1347-6947
• The Transcript Level of Katanin Gene is Increased Transiently in Response to Changes in Gravitational Conditions in Azuki Bean Epicotyls　　　　　　　　　　　　　　　
  Soga Kouichi; Kotake Toshihisa; Wakabayashi Kazuyuki; Kamisaka Seiichiro; Hoson Takayuki
  Biological Sciences in Space, Volume：23, Number：1, First page：23, Last page：28, 2009, [Reviewed]
  English
  DOI：https://doi.org/10.2187/bss.23.23
  DOI ID：10.2187/bss.23.23, ISSN：0914-9201, CiNii Articles ID：120006369774, CiNii Books ID：AN10164806
• Arabinogalactan-proteins in the evolution of gravity resistance in land plants　　　　　　　　　　　　　　　
  Kotake T; Kitazawa K; Hirata N; Soga K; Tsumuraya Y
  Biological Sciences in Space, Volume：23, Number：3, First page：143, Last page：149, 2009, [Reviewed], [Invited]
  DOI：https://doi.org/10.2187/bss.23.143
  DOI ID：10.2187/bss.23.143
• beta-1,3 : 1,4-glucan synthase activity in rice seedlings under water　　　　　　　　　　　　　　　
  Kimpara, Tomoya; Aohara, Tsutomu; Soga, Kouichi; Wakabayashi; Kazuyuki; Hoson, Takayuki; Tsumuraya, Yoichi; Kotake, Toshihisa
  Annals of Botany, Volume：102, Number：2, First page：221, Last page：226, Aug. 2008, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1093/aob/mcn077
  DOI ID：10.1093/aob/mcn077, ISSN：0305-7364, ORCID：20757425, PubMed ID：18487614, Web of Science ID：WOS:000257786300007
• Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
  Kouichi Soga; Toshihisa Kotake; Kazuyuki Wakabayashi; Seiichiro Kamisaka; Takayuki Hoson
  Journal of Plant Research, Volume：121, Number：5, First page：493, Last page：498, Jul. 2008, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s10265-008-0179-3
  DOI ID：10.1007/s10265-008-0179-3, ISSN：0918-9440, eISSN：1618-0860
• Properties of family 79 β-glucuronidases that hydrolyze β-glucuronosyl and 4-O-methyl-β-glucuronosyl residues of arabinogalactan-protein　　　　　　　　　　　　　　　
  Tomoyuki Konishi; Toshihisa Kotake; Dina Soraya; Koji Matsuoka; Tetsuo Koyama; Satoshi Kaneko; Kiyohiko Igarashi; Masahiro Samejima; Yoichi Tsumuraya
  Carbohydrate Research, Volume：343, Number：7, First page：1191, Last page：1201, May 2008, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2008.03.004
  DOI ID：10.1016/j.carres.2008.03.004, ISSN：0008-6215
• Characterization of an Endo-β-1,6-Galactanase from Streptomyces avermitilis NBRC14893
  Hitomi Ichinose; Toshihisa Kotake; Yoichi Tsumuraya; Satoshi Kaneko
  Applied and Environmental Microbiology, Volume：74, Number：8, First page：2379, Last page：2383, Apr. 2008, [Reviewed]
  ABSTRACT
  
  The putative endo-β-1,6-galactanase gene from Streptomyces avermitilis was cloned and expressed in Escherichia coli , and the enzymatic properties of the recombinant enzyme were characterized. The gene consisted of a 1,476-bp open reading frame and encoded a 491-amino-acid protein, comprising an N-terminal secretion signal sequence and glycoside hydrolase family 5 catalytic module. The recombinant enzyme, Sa1,6Gal5A, catalyzed the hydrolysis of β-1,6-linked galactosyl linkages of oligosaccharides and polysaccharides. The enzyme produced galactose and a range of β-1,6-linked galacto-oligosaccharides, predominantly β-1,6-galactobiose, from β-1,6-galactan chains. There was a synergistic effect between the enzyme and Sa1,3Gal43A in degrading tomato arabinogalactan proteins. These results suggest that Sa1,6Gal5A is the first identified endo-β-1,6-galactanase from a prokaryote.
  American Society for Microbiology, Scientific journal
  DOI：https://doi.org/10.1128/aem.01733-07
  DOI ID：10.1128/aem.01733-07, ISSN：0099-2240, eISSN：1098-5336
• A bifunctional enzyme with L-fucokinase and GDP-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis　　　　　　　　　　　　　　　
  Kotake, Toshihisa; Hojo, Sachiko; Tajima, Noriaki; Matsuoka, Koji; Koyama, Tetsuo; Tsumuraya, Yoichi
  Journal of Biological Chemistry, Volume：283, Number：13, First page：8125, Last page：8135, Mar. 2008, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1074/jbc.M710078200
  DOI ID：10.1074/jbc.M710078200, ISSN：0021-9258, ORCID：20757424, Web of Science ID：WOS:000254288000009
• Properties and Physiological Functions of UDP-Sugar Pyrophosphorylase inArabidopsis
  Toshihisa KOTAKE; Sachiko HOJO; Daisuke YAMAGUCHI; Tsutomu AOHARA; Tomoyuki KONISHI; Yoichi TSUMURAYA
  Bioscience, Biotechnology, and Biochemistry, Volume：71, Number：3, First page：761, Last page：771, Mar. 2007, [Reviewed], [Lead]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1271/bbb.60605
  DOI ID：10.1271/bbb.60605, ISSN：0916-8451, eISSN：1347-6947
• Chain elongation of pectic β-(1→4)-galactan by a partially purified galactosyltransferase from soybean (Glycine max Merr.) hypocotyls
  Tomoyuki Konishi; Toshihisa Kotake; Yoichi Tsumuraya
  Planta, Volume：226, Number：3, First page：571, Last page：579, Mar. 2007, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s00425-007-0505-3
  DOI ID：10.1007/s00425-007-0505-3, ISSN：0032-0935, eISSN：1432-2048
• Degradation of the carbohydrate moieties of arabinogalactan-proteins by microbial beta-glucunonidases　　　　　　　　　　　　　　　
  Konishi Tomoyuki; Kotake Toshihisa; Soraya Dina; Kaneko Satoshi; Igarashi Kiyohiko; Samejima Masahiro; Tsumuraya Yoichi
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S80, 2007, [Reviewed]
  DOI：https://doi.org/10.14841/jspp.2007.0.255.0
  DOI ID：10.14841/jspp.2007.0.255.0, ISSN：0032-0781, Web of Science ID：WOS:000245922700316
• Characterization of an Exo-β-1,3-D-galactanase fromStreptomyces avermitilisNBRC14893 Acting on Arabinogalactan-Proteins
  Hitomi ICHINOSE; Toshihisa KOTAKE; Yoichi TSUMURAYA; Satoshi KANEKO
  Bioscience, Biotechnology, and Biochemistry, Volume：70, Number：11, First page：2745, Last page：2750, Nov. 2006, [Reviewed]
  Informa UK Limited, Scientific journal
  DOI：https://doi.org/10.1271/bbb.60365
  DOI ID：10.1271/bbb.60365, ISSN：0916-8451, eISSN：1347-6947
• An alpha-L-arabinofuranosidase/beta-D-xylosidase from immature seeds of radish (Raphanus sativus L.)
  Kotake T, Tsuchiya K, Aohara T, Konishi T, Kaneko S, Igarashi K, Samejima M, Tsumuraya Y.
  Journal of Experimental Botany, Volume：57, Number：10, First page：2353, Last page：2362, May 2006, [Reviewed], [Lead, Corresponding]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/jxb/erj206
  DOI ID：10.1093/jxb/erj206, ISSN：0022-0957, eISSN：1460-2431
• Characterization of an Exo-β-1,3-Galactanase from Clostridium thermocellum
  Hitomi Ichinose; Atsushi Kuno; Toshihisa Kotake; Makoto Yoshida; Kazuo Sakka; Jun Hirabayashi; Yoichi Tsumuraya; Satoshi Kaneko
  Applied and Environmental Microbiology, Volume：72, Number：5, First page：3515, Last page：3523, May 2006, [Reviewed]
  ABSTRACT
  
  A gene encoding an exo-β-1,3-galactanase from Clostridium thermocellum , Ct1,3Gal43A, was isolated. The sequence has similarity with an exo-β-1,3-galactanase of Phanerochaete chrysosporium (Pc1,3Gal43A). The gene encodes a modular protein consisting of an N-terminal glycoside hydrolase family 43 (GH43) module, a family 13 carbohydrate-binding module (CBM13), and a C-terminal dockerin domain. The gene corresponding to the GH43 module was expressed in Escherichia coli , and the gene product was characterized. The recombinant enzyme shows optimal activity at pH 6.0 and 50°C and catalyzes hydrolysis only of β-1,3-linked galactosyl oligosaccharides and polysaccharides. High-performance liquid chromatography analysis of the hydrolysis products demonstrated that the enzyme produces galactose from β-1,3-galactan in an exo-acting manner. When the enzyme acted on arabinogalactan proteins (AGPs), the enzyme produced oligosaccharides together with galactose, suggesting that the enzyme is able to accommodate a β-1,6-linked galactosyl side chain. The substrate specificity of the enzyme is very similar to that of Pc1,3Gal43A, suggesting that the enzyme is an exo-β-1,3-galactanase. Affinity gel electrophoresis of the C-terminal CBM13 did not show any affinity for polysaccharides, including β-1,3-galactan. However, frontal affinity chromatography for the CBM13 indicated that the CBM13 specifically interacts with oligosaccharides containing a β-1,3-galactobiose, β-1,4-galactosyl glucose, or β-1,4-galactosyl N -acetylglucosaminide moiety at the nonreducing end. Interestingly, CBM13 in the C terminus of Ct1,3Gal43A appeared to interfere with the enzyme activity toward β-1,3-galactan and α- l -arabinofuranosidase-treated AGP.
  American Society for Microbiology, Scientific journal
  DOI：https://doi.org/10.1128/aem.72.5.3515-3523.2006
  DOI ID：10.1128/aem.72.5.3515-3523.2006, ISSN：0099-2240, eISSN：1098-5336
• An exo-beta-1,3-galactanase having a novel beta-1,3-galactan-binding module from Phanerochaete chrysosporium　　　　　　　　　　　　　　　
  Ichinose, H.; Yoshida, M.; Kotake, T.; Kuno, A.; Igarashi, K.; Tsumuraya, Y.; Samejima, M.; Hirabayashi, J.; Kobayashi, H.; Kaneko, S.
  Journal of Biological Chemistry, Volume：280, Number：27, First page：25820, Last page：25829, Jul. 2005, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1074/jbc.M501024200
  DOI ID：10.1074/jbc.M501024200, ISSN：0021-9258, ORCID：20757435, Web of Science ID：WOS:000230207900063
• Molecular Cloning of a β-Galactosidase from Radish That Specifically Hydrolyzes β-(1→3)- and β-(1→6)-Galactosyl Residues of Arabinogalactan Protein
  Toshihisa Kotake; Soraya Dina; Tomoyuki Konishi; Satoshi Kaneko; Kiyohiko Igarashi; Masahiro Samejima; Yoko Watanabe; Kazumasa Kimura; Yoichi Tsumuraya
  Plant Physiology, Volume：138, Number：3, First page：1563, Last page：1576, Jul. 2005, [Reviewed], [Lead, Corresponding]
  
  A basic β-galactosidase with high specificity toward β-(1→3)- and β-(1→6)-galactosyl residues was cloned from radish (Raphanus sativus) plants by reverse transcription-PCR. The gene, designated RsBGAL1, contained an open reading frame consisting of 2,532 bp (851 amino acids). It is expressed in hypocotyls and young leaves. RsBGAL1 was highly similar to β-galactosidases having exo-β-(1→4)-galactanase activity found in higher plants and belongs to family 35 of the glycosyl hydrolases. Recombinant RsBGAL1 was expressed in Pichia pastoris and purified to homogeneity. The recombinant enzyme specifically hydrolyzed β-(1→3)- and β-(1→6)-galactooligosaccharides, the same substrates as the native enzyme isolated from radish seeds (Sekimata et al., 1989). It split off about 90% of the carbohydrate moieties of an arabinogalactan protein extracted from radish roots in concerted action with microbial α-l-arabinofuranosidase and β-glucuronidase. These results suggest that RsBGAL1 is a new kind of β-galactosidase with different substrate specificity than other β-galactosidases that exhibit exo-β-(1→4)-galactanase activity. The C-terminal region (9.6 kD) of RsBGAL1 is significantly similar to the Gal lectin-like domain, but this region is not retained in the native enzyme. Assuming posttranslational processing of RsBGAL1 with elimination of the Gal lectin-like domain results in a protein consisting of two subunits with molecular masses of 46 and 34 kD (calculated from the RsBGAL1 gene sequence). This is in good agreement with the SDS-PAGE and matrix-assisted laser desorption/ionization-time-of flight mass spectrometry measurements for subunits of the native enzyme (45 and 34 kD) and may thus partially explain the formation process of the native enzyme.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1104/pp.105.062562
  DOI ID：10.1104/pp.105.062562, ISSN：0032-0889, eISSN：1532-2548
• Mode of Action of β-Glucuronidase fromAspergillus nigeron the Sugar Chains of Arabinogalactan-Protein
  Md. Ashraful HAQUE; Toshihisa KOTAKE; Yoichi TSUMURAYA
  Bioscience, Biotechnology, and Biochemistry, Volume：69, Number：11, First page：2170, Last page：2177, Jan. 2005, [Reviewed]
  Informa UK Limited, Scientific journal
  DOI：https://doi.org/10.1271/bbb.69.2170
  DOI ID：10.1271/bbb.69.2170, ISSN：0916-8451, eISSN：1347-6947
• Biosynthesis of (1 -> 3),(1 -> 4)-beta-glucan in developing endosperms of barley (Hordeum vulgare)　　　　　　　　　　　　　　　
  Tsuchiya, K.; Urahara, T.; Konishi, T.; Kotake, T.; Tohno-oka, T.; Komae, K.; Kawada, N.; Tsumuraya, Y.
  Physiologia Plantarum, Volume：125, Number：2, First page：181, Last page：191, 2005, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1111/j.1399-3054.2005.00558.x
  DOI ID：10.1111/j.1399-3054.2005.00558.x, ORCID：20757436, Web of Science ID：WOS:000231677000004
• UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts　　　　　　　　　　　　　　　
  Kotake, T.; Yamaguchi, D.; Ohzono, H.; Hojo, S.; Kaneko, S.; Ishida, HK; Tsumuraya, Y.
  Journal of Biological Chemistry, Volume：279, Number：44, First page：45728, Last page：45736, Oct. 2004, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1074/jbc.M408716200
  DOI ID：10.1074/jbc.M408716200, ISSN：0021-9258, ORCID：20757443, Web of Science ID：WOS:000224694900055
• Biosynthesis of pectic galactan by membrane-bound galactosyltransferase from soybean ( Glycine max Merr.) seedlings
  Tomoyuki Konishi; Terukazu Mitome; Hiroyuki Hatsushika; Md. Ashraful Haque; Toshihisa Kotake; Yoichi Tsumuraya
  Planta, Volume：218, Number：5, First page：833, Last page：842, Mar. 2004, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s00425-003-1163-8
  DOI ID：10.1007/s00425-003-1163-8, ISSN：0032-0935, eISSN：1432-2048
• Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-beta-(1 6)-galactanase gene
  Toshihisa KOTAKE; Satoshi KANEKO; Aya KUBOMOTO; Md. Ashraful HAQUE; Hideyuki KOBAYASHI; Yoichi TSUMURAYA
  Biochemical Journal, Volume：377, Number：3, First page：749, Last page：755, Feb. 2004, [Reviewed], [Lead, Corresponding]
  A gene encoding endo-β-(1→6)-galactanase from Trichoderma viride was cloned by reverse transcriptase–PCR and expressed in Escherichia coli. The gene contained an open reading frame consisting of 1437 bp (479 amino acids). The deduced amino acid sequence of the protein showed little similarity with other known glycoside hydrolases. A signal sequence (20 amino acids) was found at the N-terminal region of the protein and the molecular mass of the mature form was calculated to be 50.488 kDa. The gene product expressed in E. coli as a recombinant protein fused with thioredoxin and His6 tags had almost the same substrate specificity and mode of action as native enzyme purified from a commercial cellulase preparation of T. viride, i.e. recombinant enzyme endo-hydrolysed β-(1→6)-galacto-oligomers with a DP (degree of polymerization) higher than 3, and it could also hydrolyse α-l-arabinofuranosidase-treated arabinogalactan protein from radish. It produced β-(1→6)-galacto-oligomers ranging from DP 2 to at least 8 at the initial hydrolysis stage and galactose and β-(1→6)-galactobiose as the major products at the final reaction stage. These results indicate that the cloned gene encodes an endo-β-(1→6)-galactanase. As far as we know, this is the first time an endo-β-(1→6)-galactanase has been cloned.
  Portland Press Ltd., Scientific journal
  DOI：https://doi.org/10.1042/bj20031145
  DOI ID：10.1042/bj20031145, ISSN：0264-6021, eISSN：1470-8728
• A b-(14)-xylosyltransferase involved in the synthesis of arabinoxylans in developing barley endosperms　　　　　　　　　　　　　　　
  Urahara, T.; Tsuchiya, K.; Kotake, T.; Tohno-oka, T.; Komae, K.; Kawada, N.; Tsumuraya, Y.
  Physiologia Plantarum, Volume：122, Number：2, First page：169, Last page：180, 2004, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1111/j.0031-9317.2004.00390.x
  DOI ID：10.1111/j.0031-9317.2004.00390.x, ORCID：20757440, Web of Science ID：WOS:000224068300002
• Arabidopsis TERMINAL FLOWER 2 Gene Encodes a Heterochromatin Protein 1 Homolog and Represses both FLOWERING LOCUS T to Regulate Flowering Time and Several Floral Homeotic Genes
  Toshihisa Kotake; Shinobu Takada; Kenji Nakahigashi; Masaaki Ohto; Koji Goto
  Plant and Cell Physiology, Volume：44, Number：6, First page：555, Last page：564, Jun. 2003, [Reviewed], [Lead]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcg091
  DOI ID：10.1093/pcp/pcg091, ISSN：0032-0781, eISSN：1471-9053
• Expression and Function of Cell Wall-Bound Cationic Peroxidase in Asparagus Somatic Embryogenesis
  Hiroyuki Takeda; Toshihisa Kotake; Naoki Nakagawa; Naoki Sakurai; Donald J. Nevins
  Plant Physiology, Volume：131, Number：4, First page：1765, Last page：1774, Apr. 2003, [Reviewed]
  Abstract
  
  Cultured asparagus (Asparagus officinalis L. cv Y6) cells induced to regenerate into whole plants through somatic embryogenesis secreted a 38-kD protein into cell walls. The full-length cDNA sequence of this protein (Asparagus officinalisperoxidase 1 [AoPOX1]) determined by reverse transcriptase-polymerase chain reaction showed similarity with plant peroxidases.AoPOX1 transcripts were particularly abundant during early somatic embryogenesis. To evaluate the in vivo function of AoPOX1 protein, purified recombinant AoPOX1 protein was reacted with a series of phenolic substrates. The AoPOX1 protein was effective in the metabolism of feruloyl (o-methoxyphenol)-substituted substrates, including coniferyl alcohol. The reaction product of coniferyl alcohol was fractionated and subjected to gas chromatography-mass spectrometry analysis and 1H-nuclear magnetic resonance analysis, indicating that the oxidation product of coniferyl alcohol in the presence of AoPOX1 was dehydrodiconiferyl alcohol. The concentration of dehydrodiconiferyl alcohol in the cultured medium of the somatic embryos was in the range of 10−8  m. Functions of the AoPOX1 protein in the cell differentiation are discussed.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1104/pp.102.014654
  DOI ID：10.1104/pp.102.014654, ISSN：0032-0889, eISSN：1532-2548
• Small complex‐type N‐linked glycans are attached to cell‐wall bound exo‐β‐glucanases of both mung bean and barley seedlings
  Toshihisa Kotake; Akiko Tonari; Masaya Ohta; Fumito Matsuura; Naoki Sakurai
  Physiologia Plantarum, Volume：112, Number：3, First page：308, Last page：314, Jul. 2001, [Reviewed], [Lead]
  N‐linked glycans of wall‐bound exo‐β‐glucanases from mung bean and barley seedlings, namely Mung‐ExoI and Barley‐ExoII, were characterized. The N‐linked glycans of Mung‐ExoI and Barley‐ExoII were liberated by gas‐phase hydrazinolysis followed by re‐N‐acetylation. Their structures were determined by two‐dimensional sugar‐mapping analysis and MALDI‐TOF mass spectrometry. N‐glycans from both glucanases were of paucimannosidic‐type (small complex‐type) structures, Manα1‐6(±Manα1‐3)(Xylβ1‐2)Manβ1‐4GlcNAcβ1‐4(±Fucα1‐3) GlcNAc, which are known as typical vacuole‐type N‐glycans. The results suggest that N‐glycans of cell‐wall glucanase were produced by partial trimming of complex‐type N‐glycans by exoglycosidases during its transport from Golgi apparatus to cell walls or in the cell walls.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1034/j.1399-3054.2001.1120302.x
  DOI ID：10.1034/j.1399-3054.2001.1120302.x, ISSN：0031-9317, eISSN：1399-3054
• Role of wall-bound beta-glucanases in regulating tip-growth of Lilium longiflorum pollen tubes　　　　　　　　　　　　　　　
  Li, YQ; Kotake, T.; Sakurai, N.; Zhao, NM; Liu, Q.
  Acta Botanica Sinica, Volume：43, Number：5, First page：461, Last page：468, 2001, [Reviewed]
  English, Scientific journal
  ORCID：20757407, Web of Science ID：WOS:000169092100002
• Auxin-induced elongation growth and expressions of cell wall-bound exo- and endo-beta-glucanases in barley coleoptiles　　　　　　　　　　　　　　　
  Kotake, T.; Nakagawa, N.; Takeda, K.; Sakurai, N.
  Plant and Cell Physiology, Volume：41, Number：11, First page：1272, Last page：1278, Nov. 2000, [Reviewed], [Lead]
  Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcd056
  DOI ID：10.1093/pcp/pcd056, ISSN：0032-0781, eISSN：1471-9053, ORCID：20757409, Web of Science ID：WOS:000165474200013
• Characterization and function of wall-bound exo-β-glucanases of Lilium longiflorum pollen tubes
  Toshihisa Kotake; YiQin. Li; Mai Takahashi; Naoki. Sakurai
  Sexual Plant Reproduction, Volume：13, Number：1, First page：1, Last page：9, Jul. 2000, [Reviewed], [Lead]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s004970000036
  DOI ID：10.1007/s004970000036, ISSN：0934-0882
• Purification and characterization of wall-bound exo-1,3-β-D-glucanase from barley (Hordeum vulgare L.) seedlings
  Kotake, T.; Nakagawa, N.; Takeda, K.; Sakurai, N.
  Plant and Cell Physiology, Volume：38, Number：2, First page：194, Last page：200, Jan. 1997, [Reviewed], [Lead]
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/oxfordjournals.pcp.a029152
  DOI ID：10.1093/oxfordjournals.pcp.a029152, ISSN：0032-0781, eISSN：1471-9053, ORCID：63916535, SCOPUS ID：0031080526

• Multi-directional planar expansion of pavement cells facilitates round, fan-like cotyledon morphogenesis in Arabidopsis thaliana　　　　　　　　　　　　　　　
  菊川琴美; 今村寿子; 曽我康一; 小竹敬久; 檜垣匠
  日本植物生理学会年会(Web), Volume：64th, 2023
  J-Global ID：202302263140715376
• Metabolome analysis revealed each brittle culm mutant-specific metabolism in rice　　　　　　　　　　　　　　　
  宮城敦子; 宮城敦子; 森和久; 石川寿樹; 大久保智司; 安達俊輔; 大川泰一郎; 山口雅利; 小竹敬久; 川合真紀
  日本植物生理学会年会(Web), Volume：64th, 2023
  J-Global ID：202302234679567567
• RIC1過剰発現による子葉器官および表皮細胞の形態変化　　　　　　　　　　　　　　　
  菊川琴美; 曽我康一; 今村寿子; 小竹敬久; 檜垣匠
  Volume：86th, 2022
  J-Global ID：202302270083809125
• Exploring the mechanism of smooth leaf morphogenesis by analyzing RIC1-overexpressors　　　　　　　　　　　　　　　
  菊川琴美; 曽我康一; 今村寿子; 小竹敬久; 檜垣匠
  バイオイメージング, Volume：31, Number：2, 2022
  ISSN：1342-2634, J-Global ID：202202269025007659
• Changes in cotyledon shape and pavement cell morphology in RIC1-overexpressos　　　　　　　　　　　　　　　
  菊川琴美; 曽我康一; 今村寿子; 小竹敬久; 檜垣匠
  日本植物生理学会年会(Web), Volume：63rd, 2022
  J-Global ID：202202263500470129
• イネのカマイラズ変異体における代謝変動　　　　　　　　　　　　　　　
  宮城敦子; 森和久; 大久保智司; 安達俊輔; 大川泰一郎; 石川寿樹; 山口雅利; 小竹敬久; 川合真紀
  Volume：85th, 2021
  J-Global ID：202302210880983493
• KONJAC1タンパク質のグルコマンナン合成における役割　　　　　　　　　　　　　　　
  西垣南歩; 吉見圭永; 國枝正; 高橋大輔; 円谷陽一; 小竹敬久
  Volume：84th, 2020
  J-Global ID：202302250366761242
• Characteristics of a Fusarium oxysporum 4-O-α-L-rhamnosyl-β-D-glucuronidase acting on the terminal structure of gum arabic side chain　　　　　　　　　　　　　　　
  吉條美由; 近藤辰哉; 中屋愼; 中屋愼; 竹中重雄; 小竹敬久; 中澤昌美; 上田光宏; 阪本龍司
  日本農芸化学会大会講演要旨集(Web), Volume：2020, 2020
  ISSN：2186-7976, J-Global ID：202002272321338543
• 白色腐朽菌Phanerochaete chrysosporium由来GHファミリー43エキソ-ガラクタナーゼの構造解析　　　　　　　　　　　　　　　
  松山佳織; 岸根尚美; 藤本瑞; 砂川直輝; 小竹敬久; 円谷陽一; 鮫島正浩; 鮫島正浩; 五十嵐圭日子; 金子哲
  Volume：10, Number：4, 2020
  ISSN：2185-6427, J-Global ID：202102235409737243
• ゼニゴケにおけるEndoglucanase16の機能解析　　　　　　　　　　　　　　　
  石田光南; 黒羽剛; 石崎公庸; 檜垣匠; 楢本悟史; 横山隆亮; 小竹敬久; 西谷和彦
  Volume：60th, 2019
  J-Global ID：201902225062471345
• 宇宙の微小重力環境におけるシロイヌナズナ胚軸の成長と表層微小管動態―Aniso Tubule宇宙実験
  曽我康一; 山崎千秋; 鎌田源司; 谷川直樹; 笠原春夫; 矢野幸子; 湖城恵; 朽名夏麿; 朽名夏麿; 加藤壮英; 橋本隆; 小竹敬久; 若林和幸; 保尊隆享
  日本植物学会大会研究発表記録, Volume：81st, First page：215, 01 Sep. 2017
  Japanese
  J-Global ID：201702213496858490
• Roles of MAP65-1 and BPP1 in Gravity Resistance of Arabidopsis hypocotyls　　　　　　　　　　　　　　　
  Murakami Mana; Soga Kouichi; Kotake Toshihisa; Kato Takehide; Hashimoto Takashi; Wakabayashi Kazuyuki; Hoson Takayuki
  Biological Sciences in Space (Web), Volume：30, 2016
  ISSN：1349-967X, J-Global ID：201702259463475836
• 産学官連携による地域農・畜・水産物活用のための機能性食品開発研究を追う(42)埼玉大麦プロジェクトの進展　　　　　　　　　　　　　　　
  円谷 陽一; 小竹 敬久; 川嶋 かほる
  Volume：51, Number：1, First page：66, Last page：69, Jan. 2016
  Japanese
  ISSN：0911-3932, CiNii Articles ID：40020705213, CiNii Books ID：AN10051049
• 埼玉大麦プロジェクトの進展　　　　　　　　　　　　　　　
  円谷陽一; 小竹敬久; 川嶋かほる; 大久保俊彦; 東海林義和
  Volume：51, First page：66, Last page：69, 2016
  Japanese
• 真菌由来AGP糖鎖分解酵素を導入したシロイヌナズナの形質　　　　　　　　　　　　　　　
  吉見圭永; 吉村真美; 八鍬頼誠; 芝野誠二; 出崎能丈; 澁谷直人; 円谷陽一; 小竹敬久
  Volume：79th, 2015
  J-Global ID：201502216160625700
• Da-7 Bifidobacterium longum由来のβ-1,6-ガラクタン側鎖分解酵素群の機能解析(ヘミセルラーゼ,一般講演,日本応用糖質科学会平成26年度大会(第63回))　　　　　　　　　　　　　　　
  坂本 彩美; 下川 倫子; 小竹 敬久; 円谷 陽一; 北原 兼文; 藤田 清貴
  Volume：4, Number：3, First page：B54, 20 Aug. 2014
  Japanese
  ISSN：2185-6427, CiNii Articles ID：110009843642, CiNii Books ID：AA12509099
• エノキタケ由来AGPコアタンパク質分解酵素の精製　　　　　　　　　　　　　　　
  吉見圭永; 菅原優美; 金子哲; 五十嵐圭日子; 小竹敬久; 円谷陽一
  Volume：55th, 2014
  J-Global ID：201402255283240550
• アラビノガラクタン-プロテインのコアタンパク質に作用するプロテアーゼの精製　　　　　　　　　　　　　　　
  吉見圭永; 菅原優美; 堀千明; 五十嵐圭日子; 堂前直; 堂前直; 金子哲; 小竹敬久; 円谷陽一
  Volume：78th, 2014
  J-Global ID：201402279306931977
• 宇宙の微小重力環境で生育したイネ芽生えにおける細胞壁フェノール化合物形成
  若林和幸; 曽我康一; 保尊隆享; 小竹敬久; 山崎丘; 東端晃; 石岡憲昭; 嶋津徹; 福井啓二; 長田郁子; 笠原春夫; 鎌田源司
  日本植物学会大会研究発表記録, Volume：77th, First page：203, 20 Aug. 2013
  Japanese
  J-Global ID：201302208474286228
• Development and Promotion of "Functional Barley Foods", highly acceptable in consumers　　　　　　　　　　　　　　　
  Yoichi TSUMURAYA; Toshihisa KOTAKE; Kaoru KAWASHIMA; Yoshikazu SHOJI
  Volume：1, Number：6, First page：23, Last page：26, Jun. 2013
  Japanese, Introduction other
  ISSN：2187-4948, CiNii Articles ID：10031159386, CiNii Books ID：AA12598816
• 健康機能性に着目した大麦食品の開発　　　　　　　　　　　　　　　
  円谷陽一; 小竹敬久; 川嶋かほる; 東海林義和
  Volume：48, Number：7, First page：89, Last page：91, 2013
  Japanese
  ISSN：0911-3932, CiNii Articles ID：40019730927, CiNii Books ID：AN10051049
• AGPの糖鎖主鎖を分解するエンド-β-1,3-ガラクタナーゼ　　　　　　　　　　　　　　　
  小竹敬久; 平田尚弘; 出木雄太; 石黒真希; 一ノ瀬仁美; 金子哲; 五十嵐圭日子; 鮫島正浩; 円谷陽一
  Volume：75th, 2011
  J-Global ID：201102217514420113
• アラビノガラクタン-プロテイン糖鎖を分解するエンド-ガラクタナーゼ　　　　　　　　　　　　　　　
  平田尚弘; 出木雄太; 北澤仁成; 高田遼平; 小竹敬久; 一ノ瀬仁美; 金子哲; 石黒真希; 五十嵐圭日子; 鮫島正弘; 円谷陽一
  Volume：30th, 2011
  J-Global ID：201102271678162594
• Expression of arabinogalactan-proteins under hypergravity conditions　　　　　　　　　　　　　　　
  Kotake Toshihisa; Igarashi Shun; Tsumuraya Yoichi; Soga Kouichi; Wakabayashi Kazuyuki; Hoson Takayuki
  Number：25, Mar. 2009
  Arabinogalactan-proteins (AGPs) are a family of proteoglycans found in the plasma membrane and cell walls of higher plants. AGPs are implicated in many physiological processes such as cell elongation, cell-to-cell signaling, cell adhesion, cell death, and stress responses. The effects of hypergravity on expression of AGP species were examined using AGPs visualized with GFP. The expression level of a classical AGP significantly decreased in the influorescence meristem under hypergravity conditions. The expression level recovered within 2 hours after transfer to 1 g condition. The results indicate that expression level of the AGP is regulated by the magnitude of gravity.
  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA), Japanese
  CiNii Articles ID：120006830492, CiNii Books ID：AN10324210
• Alteration of carbohydrate moieties of AGP with glycosyl hydrolases　　　　　　　　　　　　　　　
  Kotake Toshihisa; Takata Ryohei; Kitazawa Kiminari; Kaneko Satoshi; Igarashi Kiyohiko; Samejima Masahiro; Tsumuraya Yoichi
  Plant and Cell Physiology Supplement, Volume：2009, Number：0, First page：483, Last page：483, 2009
  Arabinogalactan-proteins (AGPs) are a family of proteoglycans found in plasma membrane and cell walls of higher plants. As backbones of the carbohydrate moieties, AGPs commonly have beta-3,6-galactans, to which other auxiliary sugars such as L-arabinose (L-Ara), glucuronic acid (GlcA), and 4-O-methyl-GlcA (4-Me-GlcA) are attached. Although a variety of proteins are expected to undergo the arabinogalactan (AG) modification in vivo, the physiological functions of AG moieties remain obscure. In the present study, the structure of carbohydrate moieties of AGPs was altered with fungal alpha-L-arabinofuranosidase (Arafase) and beta-glucuronidase (GlcAase). Recombinant Arafase (rArafase) released L-Ara from AGP from radish roots in vitro. Recombinant GlcAase acted on GlcA and 4-Me-GlcA residues of the AGP in the collaborative manner with rArafase while it failed to act alone. Transgenic <I>Arabidopsis</I> plants expressing the Arafase and GlcAase exhibited higher activities of Arafase and GlcAase than wild-type plants. <br><br>Konishi et al., (2008) Carbohydr. Res. 343, 1191-1201
  日本植物生理学会
  J-Global ID：200902251712786030, CiNii Articles ID：130006990627
• Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls　　　　　　　　　　　　　　　
  SOGA Kouichi; KOTAKE Toshihisa; WAKABAYASHI Kazuyuki; KAMISAKA Seiichiro; HOSON Takayuki
  Journal of plant research, Volume：121, Number：5, First page：493, Last page：498, 01 Sep. 2008
  English
  ISSN：0918-9440, CiNii Articles ID：10022023402, CiNii Books ID：AA10900218
• Effects of gravity on the expression of arabinogalactan-proteins in Arabidopsis
  Kotake Toshihisa; Igarashi Shun; Tsumuraya Yoichi; Soga Koichi; Wakabayashi Kazuyuki; Hoson Takayuki
  First page：382, Last page：384, Mar. 2008
  ArabinoGalactan-Proteins (AGPs), a family of proteoglycans, are commonly found in higher plants and implicated in many physiological processes such as cell-to-cell signaling, cell adhesion, cell elongation, cell death, and stress responses. We examined the effects of hypergravity on expression of AGP species in Arabidopsis plants. Several AGP species including classical AGP, AG-peptide, and fasciclin-like AGP showed decreased expression under hypergravity conditions. Visualization of the AGP species with GFP (Green Fluorescent Protein) revealed their tissue-specific expression and confirmed the altered expression under hypergravity. The results suggest that AGPs play a role in the gravity response of higher plants.
  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS), Japanese
  CiNii Articles ID：120006830730
• Mechanisms of signal transformation and transduction in gravity resistance in plants
  Hoson Takayuki; Soga Koichi; Sakaki Takeshi; Muranaka Toshiya; Hashimoto Takashi; Sonobe Seiji; Kotake Toshihisa
  First page：390, Last page：393, Mar. 2008
  Resistance to the gravitational force is a principal graviresponse in plants, comparable to gravitropism. Nevertheless, only limited information has been obtained for this graviresponse. The present study aims to clarify the mechanism of signal transformation and transduction processes, with reference to the roles of the plasma membrane and cortical microtubules. Under hypergravity conditions, the expression of genes encoding 3-Hydroxy-3-MethylGlutaryl-Coenzyme A Reductase (HMGR) was up-regulated, and the level of membrane sterols was kept higher, without influencing the level or composition of other membrane components. On the other hand, the expression of the majority of alpha- and beta-tubulin genes was up-regulated and the percentage of cells with longitudinal cortical microtubules was increased by hypergravity. Also, increases in the level and molecular size of anti-gravitational xyloglucans and 1, 3, 1, 4-beta-glucans by gravity were brought about by suppression of their breakdown with their constant or enhanced biosynthesis. We have further examined the role of membrane sterols and cortical microtubules in gravity resistance using Arabidopsis mutants. Elongation growth of both hmg and tubulin mutants was suppressed even under 1 g conditions, and hypergravity did not influence their growth, suggesting that the mutation made plants hypersensitive to the gravitational force. The analysis with mutants has also revealed that the signal transduction process via membrane sterols is distinct from that via cortical microtubules. These results indicate that membrane sterols (rafts) and cortical microtubules are deeply and independently involved in maintenance of normal growth capacity against the gravitational force.
  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS), Japanese
  CiNii Articles ID：120006830733
• Functions of arabinogalactan-proteins in modification of growth anisotropy of plant roots by gravity
  小竹 敬久; 曽我 康一
  Report of Comprehensive Open Innovation Center, Saitama University, Number：1, First page：12, Last page：14, 2008
  Arabinogalactan-proteins (AGPs), a family of proteoglycans, are commonly found in higher plants and implicated in many physiological processes such as cell adhesion, cell-to-cell signaling, cell elongation, cell death, and stress responses. To address the molecular functions of AGP in the regulation of growth anisotropy of roots induced by the gravity signal, we visualized AGP species with GFP in living Arabidopsis plants. The dynamic changes of the gravity-responsive AGP species in the localization and accumulation were observed under the hypergravity condition. The results indicate that the expression of AGP genes is regulated by the magnitude of gravity. In the future study, the molecular functions of AGPs in the growth anisotropy should be clarified.
  Japanese
  ISSN：1883-8278, CiNii Articles ID：120001491448
• Arabinogalactan-proteins degrading enzymes　　　　　　　　　　　　　　　
  Ichinose H; Kotake T; Tsumuraya Y; Kaneko S
  Journal of Applied Glycoscience, Volume：55, Number：2, First page：149, Last page：155, 2008
  Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found throughout the plant kingdom. AGPs are massively glycosylated, and are implicated in diverse plant growth and development. Gum arabic and larch arabinogalactan, which are a kind of AGP, were used for food materials and additives. Carbohydrate moieties of AGPs consist of β-1,3-galactan backbone having β-1,6-galactan side-chains. Galactanases that hydrolyze β-1,3- or β-1,6-galactans are important in degrading AGPs. We succeeded in, for the first time, cloning an exo-β-1,3-galactanase and an endo-β-1,6-galactanase genes from Phanerochaete chrysosporium (Pc1,3Gal43A) and Trichoderma viride (Tv6GAL), respectively. Pc1,3Gal43A consisted of two modules resembling glycoside hydrolase family 43 (GH43) and carbohydrate binding module family 35 (CBM35). It specifically hydrolyzed only the β-1,3-linkage of two galactosyl residues in an exo-acting manner. However, it produced oligosaccharides together with galactose from AGPs, suggesting that Pc1,3Gal43A is able to accommodate β-1,6-linked galactosyl side-chains. The C-terminal CBM35 specifically bound to β-1,3-galactan. Using the Pc1,3Gal43A sequence as a query in BLAST search, we newly obtained the enzymes from Clostridium thermocellum and Streptomyces avermitilis. Both enzymes contained GH43 and CBM13. We found similar sequences not only in bacteria but also in plants. The two kinds of gene products from Arabidopsis thaliana demonstrated exo-β-1,3-galactanase activity when they were expressed in yeast. Thus, exo-β-1,3-galactanases are distributed in the fungal, bacterial and plant kingdoms. The putative endo-β-1,6-galactanase gene from S. avermitilis which was found by using Tv6GAL sequence was cloned. The recombinant enzyme catalyzed the hydrolysis of β-1,6-linked galactosyl linkages of oligosaccharides and polysaccharides in an endo-acting manner.
  The Japanese Society of Applied Glycoscience, English
  DOI：https://doi.org/10.5458/jag.55.149
  DOI ID：10.5458/jag.55.149, ISSN：1344-7882, CiNii Articles ID：10020738828, CiNii Books ID：AA11809133
• 植物の糖ヌクレオチド合成を触媒する新規なピロホスホリラーゼ : 糖1-リン酸に対して幅広い基質特異性. 各種のUDP-糖合成への利用に期待　　　　　　　　　　　　　　　
  円谷 陽一; 小竹 敬久
  Volume：45, Number：3, First page：163, Last page：164, 01 Mar. 2007
  Japanese
  ISSN：0453-073X, CiNii Articles ID：10018572759, CiNii Books ID：AN00037573
• Effects of gravity signal on beta-1,3:1,4-glucan synthase activity in rice seedlings
  Kotake Toshihisa; Kinpara Tomoya; Tsumuraya Yoichi; Soga Koichi; Wakabayashi Kazuyuki; Hoson Takayuki
  First page：371, Last page：374, Mar. 2007
  Beta-1,3:1,4-Glucan is a major cell wall component and responsible for the regulation of elongation growth in rice. We examined the effects of water immersion as a microgravity simulator on beta-1,3:1,4-glucan synthase activity in rice seedlings. Water immersion significantly reduced both amount and molecular weight of beta-1,3:1,4-glucan in the cell walls, stimulating elongation growth of the coleoptiles. The beta-1,3:1,4-glucan synthase activity in the microsome fraction of coleoptiles underwater was decreased to less than 50 percent of that of the control. These results indicate that the growth stimulation underwater is mediated by decrease in activity of beta-1,3:1,4-glucan synthase as well as increase in activity of beta-1,3:1,4-glucan degrading enzymes in the cell walls.
  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA/ISAS), Japanese
  CiNii Articles ID：120006830848
• アラビノガラクタン-プロテインの糖鎖のβ-グルクロニダーゼによる分析　　　　　　　　　　　　　　　
  古西智之; 小竹敬久; ソラヤディナ; 金子哲; 五十嵐圭日子; 鮫島正浩; 円谷陽一
  Volume：48th, 2007
  J-Global ID：200902217440780670
• アラビノガラクタン-プロテインの糖鎖のβ-グルクロニダーゼによる分解　　　　　　　　　　　　　　　
  古西智之; 小竹敬久; SORAYA Dina; 松岡浩司; 小山哲夫; 金子哲; 五十嵐圭日子; 鮫島正浩; 円谷陽一
  Volume：27th, 2007
  J-Global ID：200902224219842160
• Isolation and characterization of a novel bifunctional L-fukokinase/GDP-L-fucose pyrophosphorylase in Arabidopsis　　　　　　　　　　　　　　　
  Toshihisa Kotake; Sachiko Hojo; Yoichi Tsumuraya
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S80, Last page：S80, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922700315
• AMOR, a 70-kDa protein from the ovule, induces competence of the pollen tube to respond to the final-step guidance　　　　　　　　　　　　　　　
  INATSUGI Rie; SASAKI Narie; KOTAKE Toshihisa; TSUMURAYA Yoichi; KUROIWA Tsuneyosi; NAKANO Akihiko; HIGASHIYAMA Tetsuya
  Volume：119, First page：169, Last page：170, 01 Dec. 2006
  English
  ISSN：0918-9440, CiNii Articles ID：10019309892, CiNii Books ID：AA10900218
• Understanding the Mechanism of Gravity Resistance in Plants　　　　　　　　　　　　　　　
  Hoson Takayuki; Kamisaka Seiichiro; Takahashi Hideyuki; Yamashita Masamichi; Iida Hidetoshi; Muranaka Toshiya; Hashimoto Takashi; Sonobe Seiji; Tanimoto Eiichi; Nishitani Kazuhiko; Kotake Toshihisa; Wakabayashi Kazuyuki; Soga Kouichi
  Number：22, Mar. 2006
  The Twenty-second Space Utilization Symposium (January 17-19, 2006: Science Council of Japan, Roppongi, Tokyo, Japan)Resistance to the gravitational force is a principal graviresponse in plants, comparable to gravitropism. However, only limited information has been obtained for this graviresponse. We have organized a working group, consisting 13 members, to clarify the mechanism of gravity resistance. In this article, we report the current activity of this working group, and propose the strategies for space experiments to understand gravity resistance in plants. The process of gravity resistance consists of signal perception, transformation and transduction of a perceived signal, and response. We have shown the outline of the sequence of events leading to the development of mechanical resistance. We need to clarify the details of each step by future space experiments. For this purpose, we should design a plant cultivation chamber, applicable to not only model plants, but also other materials, including cultured cells. Also, it is required to develop some new analytical instruments, suitable for on-site analyses on orbit.Meeting sponsors: The Science Council of Japan, The Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (ISAS)(JAXA)
  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency (JAXA), Japanese
  CiNii Articles ID：120006830958, CiNii Books ID：AN10324210
• PCP Award - Arabidopsis TERMINAL FLOWER2, a Heterochromatin Protein 1 homolog, regulates flowering time.　　　　　　　　　　　　　　　
  T Kotake; S Takada; K Nakahigashi; O Masaaki; K Goto
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S24, Last page：S24, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104100094
• Phanerochaete chrysosporium β-1,3-ガラクタナーゼのクローニングとPichia pastorisでの発現,精製とその性質　　　　　　　　　　　　　　　
  一ノ瀬仁美; 吉田誠; 五十嵐圭日子; 鮫島正浩; 小竹敬久; 円谷陽一; 小林秀行; 金子哲
  Volume：2004, 2004
  J-Global ID：200902231713654896
• Molecular cloning of glycosyl hydrolases that act on the carbohydrate moieties of arabinogalactan-protein　　　　　　　　　　　　　　　
  T Kotake; K Tsuchiya; S Kaneko; MA Haque; H Kobayashi; Y Tsumuraya
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S88, Last page：S88, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700346
• Expression and Function of Cell Wall-Bound Cationic Peroxidase in Asparagus Somatic Embryogenesis　　　　　　　　　　　　　　　
  Hiroyuki Takeda; Toshihisa Kotake; Naoki Nakagawa; Naoki Sakurai; Donald J. Nevins
  Plant Physiology, Volume：131, Number：4, First page：1765, Last page：1774, 01 Apr. 2003, [Reviewed]
  English
  DOI：https://doi.org/10.1104/pp.102.014654
  DOI ID：10.1104/pp.102.014654, ISSN：0032-0889, eISSN：1532-2548, CiNii Articles ID：80015933875, PubMed ID：12692335, Web of Science ID：WOS:000185076400026
• シロイヌナズナの花成抑制因子TFL2はSwi6/HP1様タンパク質をコードし、Zn-fingerタンパク質と相互作用する (地域振興作物への病害抵抗性に関する基礎研究と遺伝子工学的手法による高度病害抵抗性付与技術の開発) -- (植物生殖成長のキープロセスを統御する分子機構の解明(CREST研究課題))　　　　　　　　　　　　　　　
  戒能 智宏; 小竹 敬久; 高田 忍
  First page：37, Last page：41, 2001
  Japanese
  CiNii Articles ID：40015237201, CiNii Books ID：AA1182211X
• シロイヌナズナのTERMINAL FLOWER2(TFL2)遺伝子はSwi6/HP1様のタンパク質をコードし、花成促進遺伝子FTの発現を負に制御している (地域振興作物への病害抵抗性に関する基礎研究と遺伝子工学的手法による高度病害抵抗性付与技術の開発) -- (植物生殖成長のキープロセスを統御する分子機構の解明(CREST研究課題))　　　　　　　　　　　　　　　
  高田 忍; 小竹 敬久; 戒能 智宏
  First page：43, Last page：47, 2001
  Japanese
  CiNii Articles ID：40015237202, CiNii Books ID：AA1182211X
• Characterization of peroxidase Secreted from Asparagus Somatic Embryo :　　　　　　　　　　　　　　　
  TAKEDA Hiroyuki; KOTAKE Toshihisa; NAKAGAWA Naoki; SAKURAI Naoki
  Plant and cell physiology, Volume：42, First page：s73, 2001
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003715425, CiNii Books ID：AA0077511X
• シロイヌナズナの花成制御因子、TFL1およびFTと相互作用する因子の探索 (地域振興作物への病害抵抗性に関する基礎研究と遺伝子工学的手法による高度病害抵抗性付与技術の開発) -- (生体高分子の植物細胞間移動に対する抵抗性を付与する技術の開発)　　　　　　　　　　　　　　　
  小竹 敬久; 後藤 弘爾
  First page：27, Last page：30, 2000
  Japanese
  CiNii Articles ID：40005384378, CiNii Books ID：AA1182211X
• CLONING OF PEROXIDASE SECRETED FROM ASPARAGUS SOMATIC EMBRYO :　　　　　　　　　　　　　　　
  TAKEDA Hiroyuki; KOHMURA Hiroyuki; KOTAKE Toshihisa; NAKAGAWA Naoki; SAKURAI Naoki
  Plant and cell physiology, Volume：41, First page：s191, 2000
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003722610, CiNii Books ID：AA0077511X
• GENE EXPRESSIONS OF EXO-, ENDO-1,3 : 1,4-AND ENDO-1,4-β-GLUCANASES IN BARLEY COLEOPTILE :　　　　　　　　　　　　　　　
  KOTAKE Toshihisa; NAKAGAWA Naoki; SAKURAI Naoki
  Plant and cell physiology, Volume：41, First page：s190, 2000
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003722603, CiNii Books ID：AA0077511X
• Hormonal expression of endo- and exo-glucanase in barley coleoptiles　　　　　　　　　　　　　　　
  KOTAKE Toshihisa; NAKAGAWA Naoki; SAKURAI Naoki
  Volume：39, First page：S79, Last page：S79, May 1998
  English
  ISSN：0032-0781, CiNii Articles ID：10003751783, CiNii Books ID：AA0077511X
• An exo-glucanase involved in elongation of pollen tube　　　　　　　　　　　　　　　
  LI Yi-Qin; KOTAKE Toshihisa; SAKURAI Naoki
  Volume：39, First page：S49, Last page：S49, May 1998
  English
  ISSN：0032-0781, CiNii Articles ID：10003751663, CiNii Books ID：AA0077511X
• Purification and characterization of wall-bound exo- and endo-glucanase in Gramineae　　　　　　　　　　　　　　　
  KOTAKE Toshihisa; NAKAGAWA Naoki; SAKURAI Naoki
  Volume：38, First page：s128, Mar. 1997
  English
  ISSN：0032-0781, CiNii Articles ID：10004345909, CiNii Books ID：AA0077511X
• Purification and characterization of β-D-glucanase involved in cell elongation　　　　　　　　　　　　　　　
  KOTAKE T.; NAKAGAWA N.; SAKURAI N.
  Plant Cell Physiol., Volume：37, First page：124, Last page：124, Mar. 1996
  English
  ISSN：0032-0781, CiNii Articles ID：10002708282, CiNii Books ID：AA0077511X

• 植物バイオマスを構成するヘミセルロースの修飾パターンと立体構造　　　　　　　　　　　　　　　
  吉見 圭永; Tryfona Theodora; 小竹 敬久; Dupree Paul, [Joint work]
  Dec. 2024, [Reviewed], ［Internationally co-authored］
  Responsible for pages：187-195
• 植物内在性の細胞壁多糖分解酵素の多様な性質とはたらき　　　　　　　　　　　　　　　
  吉田 光毅; 吉見 圭永; 高橋 大輔; 小竹 敬久, [Joint work]
  Dec. 2024, [Reviewed]
  Responsible for pages：233-241
• 環境微生物の呼吸と浄化技術の進歩　　　　　　　　　　　　　　　
  板谷光泰; 大西純一; 小竹敬久; 定家義人; 定家多美子; 田中耕生; 松岡聡; 松本幸次; 吉田健一, [Joint work]
  Aug. 2017
• 植物のプロテオグリカン，アラビノガラクタン-プロテインの構造と機能　　　　　　　　　　　　　　　
  円谷 陽一; 小竹 敬久, [Joint work]
  Aug. 2017
• Plant proteoglycan　　　　　　　　　　　　　　　
  Toshihisa Kotake
  Dec. 2016
• 植物細胞壁実験法　　　　　　　　　　　　　　　
  石井, 忠; 石水, 毅; 梅澤, 俊明; 加藤, 陽治; 岸本, 崇生; 小西, 照子; 松永, 俊朗, [Contributor]
  Feb. 2016
  Japanese, Total pages：xi, 403p
  CiNii Books：http://ci.nii.ac.jp/ncid/BB20768305
  ISBN：9784907192211, CiNii Books ID：BB20768305
• 植物細胞壁　　　　　　　　　　　　　　　
  西谷, 和彦; 梅澤, 俊明; 講談社サイエンティフィク, [Contributor]
  Mar. 2013
  Japanese, Total pages：x, 349p
  CiNii Books：http://ci.nii.ac.jp/ncid/BB11991280
  ISBN：9784061538184, CiNii Books ID：BB11991280
• 植物生体内で単糖類はどのようにリサイクルされるか?　　　　　　　　　　　　　　　
  小竹 敬久; 円谷 陽一, [Joint work]
  Dec. 2010
• Arabinogalactan-proteins degrading enzymes　　　　　　　　　　　　　　　
  Ichinose H; Kotake T; Tsumuraya Y; Kaneko S
  2008
• 植物の糖ヌクレオチド合成を触媒する新規なピロホスホリラーゼ －糖 1-リン酸に対して幅広い基質特異性、各種のUDP-糖合成への利用に期待－　　　　　　　　　　　　　　　
  円谷陽一; 小竹敬久, [Joint work]
  2007

• Oct. 2019 - Present
• Aug. 2004 - Present
• Jan. 2002 - Present
• Mar. 1996 - Present

• Molecular mechanism for synthesis of acetylated glucomannan　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2023 - 31 Mar. 2027
  Saitama University
  Grant amount(Total)：18720000, Direct funding：14400000, Indirect funding：4320000
  Grant number：23K26827
• 機能性を高めるグルコマンナン高アセチル化の分子メカニズム　　　　　　　　　　　　　　　
  Apr. 2023 - Mar. 2027
  Grant amount(Total)：18720000, Direct funding：14400000, Indirect funding：4320000
  Grant number：23H02134
• 細胞外プロテオグリカンによる植物のからだ作りの分子機構　　　　　　　　　　　　　　　
  Apr. 2023 - Mar. 2025
  Grant amount(Total)：12740000, Direct funding：9800000, Indirect funding：2940000
  Grant number：23H04302
• 科学的に探究する学習活動における効果的な生物教材と個別最適な学習プログラムの開発　　　　　　　　　　　　　　　
  Apr. 2022 - Mar. 2025
  Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
  Grant number：22K02581
• Structural and chemical properties of non-cellulosic cell wall polymers contributing to mechanical strength of plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 29 Jun. 2018 - 31 Mar. 2023
  Saitama University
  Grant amount(Total)：78520000, Direct funding：60400000, Indirect funding：18120000
  Grant number：18H05495
• Regulation of light-dependent synthesis of L-ascorbate in plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2019 - 31 Mar. 2022
  Toshihisa Kotake, Saitama University
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  L-Ascorbic acid is synthesized via nucleotide sugar GDP-mannose (GDP-Man) and GDP-L-galactose in plants. VTC1 is a GDP-mannose pyrophosphorylase catalyzing the conversion from Man 1-P to GDP-Man, a rate-determining step in this pathway. KONJAC1 (KJC1) protein has been identified as a factor increasing VTC1 activity in Arabidopsis. To examine a function of KJC1 in the stability of VTC1, transgenic Arabidopsis harboring FLAG-fused genomic VTC1 gene was generated. In kjc1 mutant, the amount of FLAG-VTC1 protien was reduced to less than 10% of wild-type Arabidopsis, corresponding to VTC1 acticity. A native form of VTC1 was extracted from Arabidopsis and applied to size-exclusion chromatography. While high VTC1 activity was detected in the high-molecular weight fraction from the wild-type plant, the activity was not detected in the fraction from kjc1 mutant. These results suggest that KJC1 protects VTC1 from protein degradation by forming a large complex in Arabidopsis.
  Grant number：19K06702
• Sensing of herbivores by damaged-cell wall recognition in plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2018 - 31 Mar. 2021
  Shinya Tomonori, Okayama University
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  Molecules in insect oral secretions, which are deposited at the feeding area on plant leaves, play an important role in the recognition of herbivores by plants. In this study, we identified a plant cell wall derived-saccharide in the oral secretion of rice armyworm (Mythimna loreyi) larvae. The saccharide triggered an array of defense responses in rice-cultured cells. Further analysis implied that the cell wall degrading enzymes involved in the saccharide release is derived from the gut microbes of M. loreyi larvae.
  Grant number：18K05558
• Metabolism and physiological importance of L-arabinose in plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2016 - 31 Mar. 2019
  Kotake Toshihisa, Saitama University
  Grant amount(Total)：4940000, Direct funding：3800000, Indirect funding：1140000
  L-Arabinose (L-Ara) is a plant-specific sugar that is not found in animals. L-Ara is synthesized as a form of UDP-L-Ara from UDP-xylose (UDP-Xyl), which is catalyzed by UDP-glucose 4-epimease (UGE) 1 in the cytosol. Although UGE1 shares high similarity of amino acid sequence with UGE2, only UGE1 has UDP-Xyl 4-epimerae (UDP-L-Ara synthase) activity. To identify the amino acid residues important for UDP-L-Ara synthase activity, recombinant UGE1 proteins with a point mutation to replace a residue with that of UGE2 were prepared in E. coli. Three amino acid residues were found to affect UDP-L-Ara synthase activity of UGE1. To the contrary, mutated UGE2 with a residue corresponding to UGE1 showed weak UDP-L-Ara synthase activity. These results suggest that these residues have changed substrate specificity of UGE1, which enabled plants to synthesize UDP-L-Ara in the cytosol.
  Grant number：16K07391
• Functions of cell wall polysaccharides and glycoproteins in the cell functions, development and differentiation in plant　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 28 Jun. 2012 - 31 Mar. 2017
  SATOH Shinobu; FURUKAWA Jun, University of Tsukuba
  Grant amount(Total)：90090000, Direct funding：69300000, Indirect funding：20790000
  1, In rice, arabinose side chain and adjustment of methylation of pectin are important for the formation of pollen cell wall and mechanical guidance of pollen tube in pistil. In abscission zone at the time of organ detachment, characteristic cell wall rearrangement occurs.
  2, In tissue reunion of cut flowering stem of Arabidopsis, XTH 20 etc. is involved in pith cell division downstream of auxin and transcription factors. Iron transporter, CLE6 peptide, xylem sap proteins and sugars regulated by shoot gibberellin, short day and low temperature are involved in shoot development.
  3, The sugar chain of arabinogalactan protein is essential for cell morphology and differentiation.
  Grant number：24114006
• A study on a mechanism for the regulation of gene expression responding to the magnitude of gravity in plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2013 - 31 Mar. 2016
  KOTAKE Toshihisa; SOGA Kouichi, Saitama University
  Grant amount(Total)：5070000, Direct funding：3900000, Indirect funding：1170000
  Using transgenic Arabidopsis harboring genomic MAP65-1-GFP gene, the expression of MAP65-1 was analyzed in the hypocotyl. The expression level in the growing region was high whereas that in basal region was low, indicating that the expression is regulated in relation to the cell elongation. The experiments using 5’region-truncated series suggested that the region from -1.1 to -0.7 kb mainly regulates the expression in the hypocotyl. The region involved in the expression responding to the magnitude of the gravity was not identified, because stable results were not obtained.
  Grant number：25514001
• A metabolic pathway for generation of GDP-glucose in higher plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), 2010 - 2012
  KOTAKE Toshihisa, Saitama University
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  The konjac(kjc)1 mutation appeared to affect glucomannan content. Recombinant KJC1 and KJC2 expressed in E. coli did not have any enzymatic activity, but they stimulated GDP-mannose/GDP-glucose synthase activity of VTC1. The results suggest that KJC1 and 2 are involved in glucomannan synthesis through the regulation of the synthesis of GDP-mannose and GDP-glucose catalyzed by VTC1.
  Grant number：22770030
• 植物が有する特異な糖ヌクレオチド代謝経路の解明　　　　　　　　　　　　　　　
  2005 - 2006
  Grant amount(Total)：1900000, Direct funding：1900000
  Grant number：17770028
• 伸長成長に関与する植物細胞壁結合タンパク質の単離・精製　　　　　　　　　　　　　　　
  1998 - 1999
  Grant amount(Total)：1800000, Direct funding：1800000
  Grant number：97J03682