Performance information

• Atypical endo-β-1,4-mannannases are necessary for normal glucomannan synthesis in Arabidopsis　　　　　　　　　　　　　　　
  Aina Kikuchi; Eriko Sato; Yoshihisa Yoshimi; Hironori Takasaki; Naho Nishigaki; Kimie Atsuzawa; Yasuko Kaneko; Masatoshi Yamaguchi; Daisuke Takahashi; Paul Dupree; Toshihisa Kotake
  Feb. 2025
  DOI：https://doi.org/10.1101/2025.02.21.639209
  DOI ID：10.1101/2025.02.21.639209, ORCID：179119572
• Defective pollen meiosis in Arabidopsis due to combined arabinan and galactan insufficiency　　　　　　　　　　　　　　　
  Takuma Kikuchi; Kouichi Soga; Toshihisa Kotake; Daisuke Takahashi
  Feb. 2025, [Last, Corresponding]
  DOI：https://doi.org/10.1101/2025.02.03.636199
  DOI ID：10.1101/2025.02.03.636199, ORCID：177630155
• Soluble sugars make a greater contribution than cell wall components to the variability of freezing tolerance in wheat cultivars
  Sushan Chowhan; Takuma Kikuchi; Momoka Ohashi; Tatsuya Kutsuno; Hiroto Handa; Toshihisa Kotake; Daisuke Takahashi
  Plant Biotechnology, Volume：41, Number：4, First page：401, Last page：415, Dec. 2024, [Reviewed], [Last, Corresponding]
  Japanese Society for Plant Cell and Molecular Biology, English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.24.0801a
  DOI ID：10.5511/plantbiotechnology.24.0801a, ISSN：1342-4580, eISSN：1347-6114
• Cytosolic UDP‐L‐arabinose synthesis by bifunctional UDP‐glucose 4‐epimerases in Arabidopsis
  Akira Umezawa; Mayuko Matsumoto; Hiroto Handa; Konatsu Nakazawa; Megumi Miyagawa; Georg J. Seifert; Daisuke Takahashi; Shinya Fushinobu; Toshihisa Kotake
  The Plant Journal, Apr. 2024, [Reviewed]
  SUMMARY
  
  L‐Arabinose (L‐Ara) is a plant‐specific sugar found in cell wall polysaccharides, proteoglycans, glycoproteins, and small glycoconjugates, which play physiologically important roles in cell proliferation and other essential cellular processes. L‐Ara is synthesized as UDP‐L‐arabinose (UDP‐L‐Ara) from UDP‐xylose (UDP‐Xyl) by UDP‐Xyl 4‐epimerases (UXEs), a type of de novo synthesis of L‐Ara unique to plants. In Arabidopsis, the Golgi‐localized UXE AtMUR4 is the main contributor to UDP‐L‐Ara synthesis. However, cytosolic bifunctional UDP‐glucose 4‐epimerases (UGEs) with UXE activity, AtUGE1, and AtUGE3 also catalyze this reaction. For the present study, we first examined the physiological importance of bifunctional UGEs in Arabidopsis. The uge1 and uge3 mutants enhanced the dwarf phenotype of mur4 and further reduced the L‐Ara content in cell walls, suggesting that bifunctional UGEs contribute to UDP‐L‐Ara synthesis. Through the introduction of point mutations exchanging corresponding amino acid residues between AtUGE1 with high UXE activity and AtUGE2 with low UXE activity, two mutations that increase relative UXE activity of AtUGE2 were identified. The crystal structures of AtUGE2 in complex forms with NAD⁺ and NAD⁺/UDP revealed that the UDP‐binding domain of AtUGE2 has a more closed conformation and smaller sugar‐binding site than bacterial and mammalian UGEs, suggesting that plant UGEs have the appropriate size and shape for binding UDP‐Xyl and UDP‐L‐Ara to exhibit UXE activity. The presented results suggest that the capacity for cytosolic synthesis of UDP‐L‐Ara was acquired by the small sugar‐binding site and several mutations of UGEs, enabling diversified utilization of L‐Ara in seed plants.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.16779
  DOI ID：10.1111/tpj.16779, ISSN：0960-7412, eISSN：1365-313X
• Structural changes in cell wall pectic polymers contribute to freezing tolerance induced by cold acclimation in plants　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kouichi Soga; Takuma Kikuchi; Tatsuya Kutsuno; Pengfei Hao; Kazuma Sasaki; Yui Nishiyama; Satoshi Kidokoro; Arun Sampathkumar; Antony Bacic; Kim L. Johnson; Toshihisa Kotake
  Current Biology, Mar. 2024, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2024.01.045
  DOI ID：10.1016/j.cub.2024.01.045, ORCID：152710373
• On the Separate Metabolisms of UDP-Sugars and GDP-Sugars in Plants
  Toshihisa Kotake; Daisuke Takahashi
  Trends in Glycoscience and Glycotechnology, Volume：36, Number：209, First page：J6, Last page：J11, Jan. 2024, [Reviewed]
  Forum: Carbohydrates Coming of Age, Scientific journal
  DOI：https://doi.org/10.4052/tigg.2303.1j
  DOI ID：10.4052/tigg.2303.1j, ISSN：0915-7352, eISSN：1883-2113
• Plant type II arabinogalactan: Structural features and modification to increase functionality　　　　　　　　　　　　　　　
  Kanika Ghosh; Daisuke Takahashi; Toshihisa Kotake
  Carbohydrate Research, Volume：529, First page：108828, Last page：108828, Jul. 2023, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2023.108828
  DOI ID：10.1016/j.carres.2023.108828, ISSN：0008-6215
• Spatiotemporal Control of Ice Crystallization in Supercooled Water via an Ultrashort Laser Impulse.　　　　　　　　　　　　　　　
  Hozumi Takahashi; Tatsuya Kono; Kosuke Sawada; Satoru Kumano; Yuka Tsuri; Mihoko Maruyama; Masashi Yoshimura; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura; Seiichiro Nakabayashi; Yusuke Mori; Yoichiroh Hosokawa; Hiroshi Y Yoshikawa
  The journal of physical chemistry letters, First page：4394, Last page：4402, May 2023, [Reviewed], ［International magazine］
  Focused irradiation with ultrashort laser pulses realized the fine spatiotemporal control of ice crystallization in supercooled water. An effective multiphoton excitation at the laser focus generated shockwaves and bubbles, which acted as an impulse for inducing ice crystal nucleation. The impulse that was localized close to the laser focus and accompanied by a small temperature elevation allowed the precise position control of ice crystallization and its observation with spatiotemporal resolution of micrometers and microseconds using a microscope. To verify the versatility of this laser method, we also applied it using various aqueous systems (e.g., plant extracts). The systematic study of crystallization probability revealed that laser-induced cavitation bubbles play a crucial role in inducing ice crystal nucleation. This method can be used as a tool for studying ice crystallization dynamics in various natural and biological phenomena.
  English, Scientific journal
  DOI：https://doi.org/10.1021/acs.jpclett.3c00414
  DOI ID：10.1021/acs.jpclett.3c00414, PubMed ID：37154425
• LSPpred Suite: Tools for Leaderless Secretory Protein Prediction in Plants
  Andrew Lonsdale; Laura Ceballos-Laita; Daisuke Takahashi; Matsuo Uemura; Javier Abadía; Melissa J. Davis; Antony Bacic; Monika S. Doblin
  Plants, Mar. 2023, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.3390/plants12071428
  DOI ID：10.3390/plants12071428, ORCID：131417758
• Cross‐disciplinary insights into the mechanisms of plant cold hardiness: From molecules to ecosystems　　　　　　　　　　　　　　　
  Guillaume Charrier; Ian R. Willick; Daisuke Takahashi
  Physiologia Plantarum, Volume：175, First page：e13901, Mar. 2023, [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13901
  DOI ID：10.1111/ppl.13901, ORCID：133007880
• Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light　　　　　　　　　　　　　　　
  Kazusato Oikawa; Shino Goto-Yamada; Yasuko Hayashi; Daisuke Takahashi; Yoshitaka Kimori; Michitaro Shibata; Kohki Yoshimoto; Atsushi Takemiya; Maki Kondo; Kazumi Hikino; Akira Kato; Keisuke Shimoda; Haruko Ueda; Matsuo Uemura; Keiji Numata; Yoshinori Ohsumi; Ikuko Hara-Nishimura; Shoji Mano; Kenji Yamada; Mikio Nishimura
  Nature Communications, Volume：13, Number：1, First page：7493, Last page：7493, Dec. 2022, [Reviewed], ［International magazine］
  Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-022-35138-z
  DOI ID：10.1038/s41467-022-35138-z, ORCID：124156494, PubMed ID：36470866
• Temporal cell wall changes during cold acclimation and deacclimation and their potential involvement in freezing tolerance and growth
  Tatsuya Kutsuno; Sushan Chowhan; Toshihisa Kotake; Daisuke Takahashi
  Physiologia Plantarum, Dec. 2022, [Reviewed], [Last, Corresponding]
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13837
  DOI ID：10.1111/ppl.13837, ISSN：0031-9317, eISSN：1399-3054, ORCID：124048822
• In vivo structural modification of type II arabinogalactans with fungal endo-β-1, 6-galactanase in Arabidopsis　　　　　　　　　　　　　　　
  Aina Kikuchi; Katsuya Hara; Yoshihisa Yoshimi; Kouichi Soga; Toshihisa Kotake
  Frontiers in Plant Science, Volume：13, Nov. 2022, [Reviewed]
  Arabinogalactan-proteins (AGPs) are mysterious extracellular glycoproteins in plants. Although AGPs are highly conserved, their molecular functions remain obscure. The physiological importance of AGPs has been extensively demonstrated with β-Yariv reagent, which specifically binds to AGPs and upon introduction into cells, causes various deleterious effects including growth inhibition and programmed cell death. However, structural features of AGPs that determine their functions have not been identified with β-Yariv reagent. It is known that AGPs are decorated with large type II arabinogalactans (AGs), which are necessary for their functions. Type II AGs consist of a β-1,3-galactan main chain and β-1,6-galactan side chains with auxiliary sugar residues such as L-arabinose and 4-O-methyl-glucuronic acid. While most side chains are short, long side chains such as β-1,6-galactohexaose (β-1,6-Gal6) also exist in type II AGs. To gain insight into the structures important for AGP functions, in vivo structural modification of β-1,6-galactan side chains was performed in Arabidopsis. We generated transgenic Arabidopsis plants expressing a fungal endo-β-1,6-galactanase, Tv6GAL, that degrades long side chains specifically under the control of dexamethasone (Dex). Two of 6 transgenic lines obtained showed more than 40 times activity of endo-β-1,6-galactanase when treated with Dex. Structural analysis indicated that long side chains such as β-1,6-Gal5 and β-1,6-Gal6 were significantly reduced compared to wild-type plants. Tv6GAL induction caused retarded growth of seedlings, which had a reduced amount of cellulose in cell walls. These results suggest that long β-1,6-galactan side chains are necessary for normal cellulose synthesis and/or deposition as their defect affects cell growth in plants.
  Frontiers Media {SA}, Scientific journal
  DOI：https://doi.org/10.3389/fpls.2022.1010492
  DOI ID：10.3389/fpls.2022.1010492, ISSN：1664-462X, ORCID：123074886
• Plasma membrane proteomic changes of Arabidopsis DRP1E during cold acclimation in association with the enhancement of freezing tolerance　　　　　　　　　　　　　　　
  Etsuko Watanabe; Mariko Kondo; Md Mostafa Kamal; Matsuo Uemura; Daisuke Takahashi; Yukio Kawamura
  Physiologia Plantarum, First page：e13820, Nov. 2022, [Reviewed], [Last, Corresponding], ［International magazine］
  The freezing tolerance of plants that live in cold regions increases after exposure to low temperature, a process termed cold acclimation (CA). During CA, restructuring of the plasma membrane (PM) is important to enhance freezing tolerance. We have previously shown that the function of DYNAMIN-RELATED PROTEIN 1E (DRP1E), which regulates endocytosis by pinching vesicles from the PM, is associated with the enhancement of freezing tolerance during CA in Arabidopsis. DRP1E is predicted to play a role in reconstituting the PM composition during CA. In this study, to test the validity of this hypothesis, we studied the changes in PM proteome patterns induced by drp1e mutation. In a detailed physiological analysis, after 3 days of CA, only young leaves showed significantly less increase in freezing tolerance in the mutant than in the wild type (WT). Using nano-liquid chromatography-tandem mass spectrometry, 496 PM proteins were identified. Among these proteins, 81 or 71 proteins were specifically altered in the WT or the mutant, respectively, in response to CA. Principal component analysis showed that the proteomic pattern differed between the WT and the mutant upon cold acclimation, suggesting that DRP1E contributes to reconstruction of the PM during CA. Cluster analysis revealed that proteins that were significantly increased in the mutant after CA were biased toward glycosylphosphatidylinositol-anchored proteins, such as fasciclin-like arabinogalactan proteins. Thus, a primary target of DRP1E-associated PM reconstruction during CA is considered to be glycosylphosphatidylinositol-anchored proteins, which may be removed from the PM by DRP1E in young leaves after 3 days of CA.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13820
  DOI ID：10.1111/ppl.13820, ORCID：122224080, PubMed ID：36335535
• Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (Solanum lycopersicum) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses
  Laura Ceballos-Laita; Daisuke Takahashi; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán; Jorge Rodríguez-Celma
  International Journal of Molecular Sciences, Volume：23, Number：7, First page：3719, Last page：3719, Mar. 2022, [Reviewed]
  Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
  {MDPI} {AG}, English, Scientific journal
  DOI：https://doi.org/10.3390/ijms23073719
  DOI ID：10.3390/ijms23073719, eISSN：1422-0067, ORCID：110644125
• Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.　　　　　　　　　　　　　　　
  Naho Nishigaki; Yoshihisa Yoshimi; Hiroaki Kuki; Tadashi Kunieda; Ikuko Hara-Nishimura; Yoichi Tsumuraya; Daisuke Takahashi; Paul Dupree; Toshihisa Kotake
  Physiologia plantarum, Volume：173, Number：3, First page：1244, Last page：1252, Aug. 2021, [Reviewed], ［International magazine］
  Cell wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases. However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear. GDP-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as substrate in the synthesis of mannan polysaccharides. GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis. Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan. Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan. On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure. These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars. This article is protected by copyright. All rights reserved.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13519
  DOI ID：10.1111/ppl.13519, PubMed ID：34380178
• Responses of the Plant Cell Wall to Sub-Zero Temperatures: A Brief Update
  Daisuke Takahashi; Ian R Willick; Jun Kasuga; David P Livingston III
  Plant and Cell Physiology, Jul. 2021, [Lead, Corresponding]
  
  Our general understanding of plant responses to sub-zero temperatures focuses on mechanisms that mitigate stress to the plasma membrane. The plant cell wall receives comparatively less attention and questions surrounding its role in mitigating freezing injury remains unresolved. Despite recent molecular discoveries that provide insight into acclimation responses, the goal of reducing freezing injury in herbaceous and woody crops remains elusive. This is likely due to the complexity associated with adaptations to low temperatures. Understanding how leaf cell walls of herbaceous annuals promote tissue tolerance to ice does not necessarily lead to understanding how meristematic tissues are protected from freezing by tissue-level barriers formed by cell walls in overwintering tree buds. In this mini-review, we provide an overview of biological ice nucleation and explain how plants control the spatiotemporal location of ice formation. We discuss how sugars and pectin side chains alleviate adhesive injury that develops at sub-zero temperatures between the matrix polysaccharides and ice. The importance of site-specific cell wall elasticity to promote tissue expansion for ice accommodation and control of porosity to impede ice growth and promote supercooling will be presented. How specific cold-induced proteins modify plant cell walls to mitigate freezing injury will also be discussed. The opinions presented in this report emphasize the importance of a plant’s developmental physiology when characterizing mechanisms of freezing survival.
  Oxford University Press ({OUP}), English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab103
  DOI ID：10.1093/pcp/pcab103, ISSN：0032-0781, eISSN：1471-9053, ORCID：96808227
• Cold Acclimation Responses of Fasciclin-like Arabinogalactan Protein Genes in A. Thaliana　　　　　　　　　　　　　　　
  TAKAHASHI Daisuke; SHIBASAKI Yukino; KOTAKE Toshihisa
  Cryobiology and Cryotechnology, Volume：67, Number：1, First page：53, Last page：57, 2021
  Cold acclimation (CA) results in alterations of plasma membrane (PM) and cell wall proteins in plants,
  which is considered to be crucial for increasing their freezing tolerance. Fasciclin-like arabinogalactan
  proteins (FLAs) are abundant glycoproteins in the PM and cell wall and have potential roles in abiotic stress
  responses via transducing signaling and modulating cell wall functions. In present study, we investigated
  temporal expression of 21 FLA genes during CA in Arabidopsis thaliana. We found that there are a variety of
  changing patterns among FLAs in response to CA. Principal component analysis clearly showed that
  expression of FLAs and its responses to CA varied considerably between shoot and root. FLAs2, 8 and 13,
  the most abundant FLA genes, exhibited increases of their expression in the early stage of CA, but its extent
  was greatly different between shoot and root. Taken together, we will discuss about the potential role of
  FLAs in response to low temperature in plants.
  Japanese Society of Cryobiology and Cryotechnology, Japanese
  DOI：https://doi.org/10.20585/cryobolcryotechnol.67.1_53
  DOI ID：10.20585/cryobolcryotechnol.67.1_53, ISSN：1340-7902, eISSN：2424-1555, CiNii Articles ID：130008054042, CiNii Books ID：AN10448734
• Structural features conserved in subclass of type II arabinogalactan
  Kengo Ito; Kurumi Fukuoka; Naho Nishigaki; Katsuya Hara; Yoshihisa Yoshimi; Hiroaki Kuki; Daisuke Takahashi; Yoichi Tsumuraya; Toshihisa Kotake
  Plant Biotechnology, Volume：37, Number：4, First page：459, Last page：463, Dec. 2020, [Reviewed]
  Japanese Society for Plant Cell and Molecular Biology, English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.20.0721a
  DOI ID：10.5511/plantbiotechnology.20.0721a, ISSN：1347-6114, eISSN：1347-6114, ORCID：85846532
• Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis.　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Andrew Lonsdale; Anunciación Abadía; Monika S Doblin; Antony Bacic; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán
  International journal of molecular sciences, Volume：21, Number：22, First page：8863, Last page：8863, Nov. 2020, [Reviewed], ［International magazine］
  Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant's response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 µM MnCl2 as control and excess Mn treatments, respectively. This approach yielded 668 proteins reliably identified and quantified. Excess Mn caused statistically significant (at p ≤ 0.05) and biologically relevant changes in relative abundance (≥2-fold increases or ≥50% decreases) in 322 proteins, with 82% of them predicted to be secretory using three different prediction tools, with more decreasing than increasing (181 and 82, respectively), suggesting that this metal stress causes an overall deactivation of metabolic pathways. Processes most affected by excess Mn were in the oxido-reductase, polysaccharide and protein metabolism classes. Excess Mn induced changes in hydrolases and peroxidases involved in cell wall degradation and lignin formation, respectively, consistent with the existence of alterations in the cell wall. Protein turnover was also affected, as indicated by the decrease in proteolytic enzymes and protein synthesis-related proteins. Excess Mn modified the redox environment of the xylem sap, with changes in the abundance of oxido-reductase and defense protein classes indicating a stress scenario. Finally, results indicate that excess Mn decreased the amounts of proteins associated with several signaling pathways, including fasciclin-like arabinogalactan-proteins and lipids, as well as proteases, which may be involved in the release of signaling peptides and protein maturation. The comparison of the proteins changing in abundance in xylem sap and roots indicate the existence of tissue-specific and systemic responses to excess Mn. Data are available via ProteomeXchange with identifier PXD021973.
  {MDPI} {AG}, English, Scientific journal
  DOI：https://doi.org/10.3390/ijms21228863
  DOI ID：10.3390/ijms21228863, ISSN：1422-0067, ORCID：84202246, PubMed ID：33238539, PubMed Central ID：PMC7700171
• Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub‐zero acclimation　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kim Johnson; Pengfei Hao; Tan Tuong; Alex; er Erban; Arun Sampathkumar; Antony Bacic; David P. Livingston; Joachim Kopka; Takeshi Kuroha; Ryusuke Yokoyama; Kazuhiko Nishitani; Ellen Zuther; Dirk K. Hincha
  Plant, Cell & Environment, Volume：44, Number：3, First page：915, Last page：930, Nov. 2020, [Reviewed], [Lead, Corresponding], ［International magazine］
  Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle. Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation. In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation). Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation. In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH). The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure. Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant. Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/pce.13953
  DOI ID：10.1111/pce.13953, ISSN：1365-3040, ORCID：83520467, PubMed ID：33190295
• Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS.　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  Methods in molecular biology (Clifton, N.J.), Volume：2139, First page：89, Last page：106, 2020, [Reviewed], [Lead], ［International magazine］
  Shotgun proteomics allows for the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. However, this method is not fully applicable for highly hydrophobic proteins with multiple transmembrane domains. In order to solve this problem, we here describe a shotgun proteomics method using nano-LC-MS/MS for proteins in the plasma membrane and plasma membrane microdomain fractions. The results obtained are easily applicable to label-free protein semiquantification.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0528-8_7
  DOI ID：10.1007/978-1-0716-0528-8_7, PubMed ID：32462580
• Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins.　　　　　　　　　　　　　　　
  Md Mostafa Kamal; Daisuke Takahashi; Takato Nakayama; Yushi Miki; Yukio Kawamura; Matsuo Uemura
  Methods in molecular biology (Clifton, N.J.), Volume：2156, First page：171, Last page：186, 2020, [Reviewed], ［International magazine］
  Plasma membrane is the primary determinant of freezing tolerance in plants because of its central role in freeze-thaw cycle. Changes in plasma membrane protein composition have been one of the major research areas in plant cold acclimation. To obtain comprehensive profiles of the plasma membrane proteomes and their changes during the cold acclimation process, a plasma membrane purification method using a dextran-polyethylene glycol two polymer system and a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for the plasma membrane proteins are described. The proteomic results obtained are further applied to label-free protein semiquantification.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0660-5_13
  DOI ID：10.1007/978-1-0716-0660-5_13, PubMed ID：32607982
• Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.　　　　　　　　　　　　　　　
  Daisuke Takahashi; Ellen Zuther; Dirk K Hincha
  Methods in molecular biology (Clifton, N.J.), Volume：2156, First page：255, Last page：268, 2020, [Reviewed], [Lead], ［International magazine］
  The cell wall has a crucial influence on the mechanical properties of plant cells. It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants. However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation. In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation. We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation. Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0660-5_17
  DOI ID：10.1007/978-1-0716-0660-5_17, PubMed ID：32607986
• Plasma membrane proteome analyses of Arabidopsis thaliana suspension-cultured cells during cold or ABA treatment: Relationship with freezing tolerance and growth phase.　　　　　　　　　　　　　　　
  Li B; Takahashi D; Kawamura Y; Uemura M
  Journal of proteomics, Volume：211, First page：103528, Last page：103528, Sep. 2019, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2019.103528
  DOI ID：10.1016/j.jprot.2019.103528, ISSN：1874-3919, PubMed ID：31541795
• Temporal proteomics of Arabidopsis plasma membrane during cold- and de-acclimation　　　　　　　　　　　　　　　
  Yushi Miki; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Journal of Proteomics, Volume：197, First page：71, Last page：81, Apr. 2019, [Reviewed], [Lead]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2018.11.008
  DOI ID：10.1016/j.jprot.2018.11.008, ISSN：1874-3919, ORCID：78380633, PubMed ID：30447334
• Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Daisuke Takahashi; Michal Gorka; Alexander Erban; Alexander Graf; Joachim Kopka; Ellen Zuther; Dirk K. Hincha
  Scientific Reports, Volume：9, Number：1, Feb. 2019, [Reviewed], [Lead]
  Abstract
  
  Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated. In addition, many plant species, such asArabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA). There is comparatively little information available about the molecular basis of SZA. However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA. Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure. While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA. However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry. These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s41598-019-38688-3
  DOI ID：10.1038/s41598-019-38688-3, ISSN：2045-2322, eISSN：2045-2322, ORCID：78380625, PubMed ID：30783145
• Data on xylem sap proteins from Mn- and Fe-deficient tomato plants obtained using shotgun proteomics　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Anunciación Abadía; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán
  Data in Brief, Volume：17, First page：512, Last page：516, Apr. 2018, [Reviewed]
  This article contains consolidated proteomic data obtained from xylem sap collected from tomato plants grown in Fe- and Mn-sufficient control, as well as Fe-deficient and Mn-deficient conditions. Data presented here cover proteins identified and quantified by shotgun proteomics and Progenesis LC-MS analyses: proteins identified with at least two peptides and showing changes statistically significant (ANOVA
  p ≤ 0.05) and above a biologically relevant selected threshold (fold ≥ 2) between treatments are listed. The comparison between Fe-deficient, Mn-deficient and control xylem sap samples using a multivariate statistical data analysis (Principal Component Analysis, PCA) is also included. Data included in this article are discussed in depth in the research article entitled “Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses” [1]. This dataset is made available to support the cited study as well to extend analyses at a later stage.
  Elsevier Inc., English, Scientific journal
  DOI：https://doi.org/10.1016/j.dib.2018.01.034
  DOI ID：10.1016/j.dib.2018.01.034, ISSN：2352-3409, PubMed ID：29876421, SCOPUS ID：85041676938
• Tissue-specific changes in apoplastic proteins and cell wall structure during cold acclimation of winter wheat crowns　　　　　　　　　　　　　　　
  Ian R Willick; Daisuke Takahashi; D Brian Fowler; Matsuo Uemura; Karen K. Tanino
  Journal of Experimental Botany, Volume：69, Number：5, First page：1221, Last page：1234, Feb. 2018, [Reviewed]
  The wheat (Triticum aestivum L.) crown is the critical organ of low temperature stress survival over winter. In cold-acclimated crowns, ice formation in the apoplast causes severe tissue disruption as it grows at the expense of intracellular water. While previous crown studies have shown the vascular transition zone (VTZ) to have a higher freezing sensitivity than the shoot apical meristem (SAM), the mechanism behind the differential freezing response is not fully understood. Cooling cold-acclimated crowns to -10 °C resulted in an absence of VTZ tetrazolium chloride staining, whereas the temperatures at which 50% of the SAM stained positive and 50% of plants recovered (LT 50) were similar after cold acclimation for 21 (-16 °C) and 42 d (-20 °C) at 4 °C. Proteomic analysis of the apoplastic fluids identified dehydrins, vernalization-responsive proteins, and cold shock proteins preferentially accumulated in the SAM. In contrast, modifications to the VTZ centered on increases in pathogenesis-related proteins, anti-freeze proteins, and sugar hydrolyzing enzymes. Fourier transform infrared spectroscopy focal plane array analysis identified the biochemical modification of the cell wall to enhance methyl-esterified cross-linking of glucuronoarabinoxylans in the VTZ. These findings indicate that the SAM and VTZ express two distinct tissue-specific apoplastic responses during cold acclimation.
  Oxford University Press, English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erx450
  DOI ID：10.1093/jxb/erx450, ISSN：1460-2431, PubMed ID：29373702, SCOPUS ID：85042625541
• Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Anunciacion Abadia; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOMICS, Volume：170, First page：117, Last page：129, Jan. 2018, [Reviewed]
  The aim of this work was to study the effects of Fe and Mn deficiencies on the xylem sap proteome of tomato using a shotgun proteomic approach, with the final goal of elucidating plant response mechanisms to these stresses. This approach yielded 643 proteins reliably identified and quantified with 70% of them predicted as secretory. Iron and Mn deficiencies caused statistically significant and biologically relevant abundance changes in 119 and 118 xylem sap proteins, respectively. In both deficiencies, metabolic pathways most affected were protein metabolism, stress/oxidoreductases and cell wall modifications. First, results suggest that Fe deficiency elicited more stress responses than Mn deficiency, based on the changes in oxidative and proteolytic enzymes. Second, both nutrient deficiencies affect the secondary cell wall metabolism, with changes in Fe deficiency occurring via peroxidase activity, and in Mn deficiency involving peroxidase, Cu-oxidase and fasciclin-like arabinogalactan proteins. Third, the primary cell wall metabolism was affected by both nutrient deficiencies, with changes following opposite directions as judged from the abundances of several glycoside-hydrolases with endo-glycolytic activities and pectin esterases. Fourth, signaling pathways via xylem involving CLE and/or lipids as well as changes in phosphorylation and N-glycosylation also play a role in the responses to these stresses.
  Biological significance
  In spite of being essential for the delivery of nutrients to the shoots, our knowledge of xylem responses to nutrient deficiencies is very limited. The present work applies a shotgun proteomic approach to unravel the effects of Fe and Mn deficiencies on the xylem sap proteome. Overall, Fe deficiency seems to elicit more stress in the xylem sap proteome than Mn deficiency, based on the changes measured in proteolytic and oxido-reductase proteins, whereas both nutrients exert modifications in the composition of the primary and secondary cell wall. Cell wall modifications could affect the mechanical and permeability properties of the xylem sap vessels, and therefore ultimately affect solute transport and distribution to the leaves. Results also suggest that signaling cascades involving lipid and peptides might play a role in nutrient stress signaling and pinpoint interesting candidates for future studies. Finally, both nutrient deficiencies seem to affect phosphorylation and glycosylation processes, again following an opposite pattern.
  ELSEVIER SCIENCE BV, English, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2017.08.018
  DOI ID：10.1016/j.jprot.2017.08.018, ISSN：1874-3919, eISSN：1876-7737, PubMed ID：28847647, Web of Science ID：WOS:000415778000011
• Plasma membrane proteomics of arabidopsis suspension-cultured cells associated with growth phase using Nano-LC-MS/MS　　　　　　　　　　　　　　　
  Bin Li; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1696, First page：185, Last page：194, 2018, [Reviewed]
  Arabidopsis thaliana suspension-cultured cells (T87 line) are important model system for studies of responses to biotic and abiotic stresses at the cellular level in vitro since the cells have certain advantages compared with the whole plant system. However, the physiological and morphological characteristics of the cells are influenced by the progress of the growth phase of cells, which may result in different stress tolerance. To obtain comprehensive proteome profiles of the plasma membrane of Arabidopsis thaliana T87 suspension-cultured cells at the lag, log, or stationary growth phase, a shotgun proteomics method using nano-LC-MS/MS is used. The results obtained indicate that proteome profiles of the plasma membrane with the progress of the growth phase of cells dynamically changed, which may be associated with the physiological and morphological characteristics of the plasma membrane of the suspension-cultured cells. The proteomics results are further applied to explain different responsive patterns in the plasma membrane to cold acclimation and ABA treatment, which lead to understanding of different freezing tolerance associated with the growth phase of the cells.
  Humana Press Inc., English, In book
  DOI：https://doi.org/10.1007/978-1-4939-7411-5_12
  DOI ID：10.1007/978-1-4939-7411-5_12, ISSN：1064-3745, PubMed ID：29086404, SCOPUS ID：85032646702
• Freezing Tolerance of Plant Cells: From the Aspect of Plasma Membrane and Microdomain　　　　　　　　　　　　　　　
  Daisuke Takahashi
  Advances in Experimental Medicine and Biology, Volume：1081, First page：61, Last page：79, 2018, [Reviewed], [Lead]
  DOI：https://doi.org/10.1007/978-981-13-1244-1_4
  DOI ID：10.1007/978-981-13-1244-1_4, ISSN：0065-2598, ORCID：78380660, PubMed ID：30288704
• Isolation of plasma membrane and plasma membrane microdomains　　　　　　　　　　　　　　　
  Anzu Minami; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1511, First page：199, Last page：212, 2017, [Reviewed]
  The plasma membrane surrounds the cytoplasm of a cell and functions as a barrier to separate the intracellular compartment from the extracellular environment. Protein and lipid components distribute nonuniformly and the components form clusters with various functions in the plasma membrane. These clusters are called as " microdomains." In plant cells, microdomains have been studied extensively because they play important roles in biotic/abiotic stress responses, cellular traffi cking, and cell wall metabolism. Here we describe a standard protocol for the isolation of the plasma membrane and microdomains from plant cells, Arabidopsis and oat.
  Humana Press Inc., English, In book
  DOI：https://doi.org/10.1007/978-1-4939-6533-5_16
  DOI ID：10.1007/978-1-4939-6533-5_16, ISSN：1064-3745, PubMed ID：27730613, SCOPUS ID：84991457583
• Cold acclimation is accompanied by complex responses of glycosylphosphatidylinositol (GPI)-anchored proteins in Arabidopsis　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：67, Number：17, First page：5203, Last page：5215, Sep. 2016, [Reviewed], [Lead]
  Cold acclimation results in changes of the plasma membrane (PM) composition. The PM is considered to contain specific lipid/protein-enriched microdomains which can be extracted as detergent-resistant plasma membrane (DRM). Previous studies in animal cells have demonstrated that glycosylphosphatidylinositol-anchored proteins (GPI-APs) can be targeted to microdomains and/or the apoplast. However, the functional significance of GPI-APs during cold acclimation in plants is not yet fully understood. In this study, we aimed to investigate the responsiveness of GPI-APs to cold acclimation treatment in Arabidopsis. We isolated the PM, DRM, and apoplast fractions separately and, in addition, GPI-AP-enriched fractions were prepared from the PM preparation. Label-free quantitative shotgun proteomics identified a number of GPI-APs (163 proteins). Among them, some GPI-APs such as fasciclin-like arabinogalactan proteins and glycerophosphoryldiester phosphodiesterase-like proteins predominantly increased in PM-and GPI-AP-enriched fractions while the changes of GPI-APs in the DRM and apoplast fractions during cold acclimation were considerably different from those of other fractions. These proteins are thought to be associated with cell wall structure and properties. Therefore, this study demonstrated that each GPI-AP responded to cold acclimation in a different manner, suggesting that these changes during cold acclimation are involved in rearrangement of the extracellular matrix including the cell wall towards acquisition of freezing tolerance.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erw279
  DOI ID：10.1093/jxb/erw279, ISSN：0022-0957, eISSN：1460-2431, PubMed ID：27471282, Web of Science ID：WOS:000384648900022
• A Shotgun Proteomic Approach Reveals That Fe Deficiency Causes Marked Changes in the Protein Profiles of Plasma Membrane and Detergent-Resistant Microdomain Preparations from Beta vulgaris Roots　　　　　　　　　　　　　　　
  Elain Gutierrez-Carbonell; Daisuke Takahashi; Sabine Luethje; Jose Antonio Gonzalez-Reyes; Sebastien Mongrand; Bruno Contreras-Moreira; Anunciacion Abadia; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOME RESEARCH, Volume：15, Number：8, First page：2510, Last page：2524, Aug. 2016, [Reviewed]
  In the present study we have used label-free shotgun proteomic analysis to examine the effects of Fe deficiency on the protein profiles of highly pure sugar beet root plasma membrane (PM) preparations and detergent-resistant membranes (DRMs), the latter as an approach to study microdomains. Altogether, 545 proteins were detected, with 52 and 68 of them changing significantly with Fe deficiency in PM and DRM, respectively. Functional categorization of these proteins showed that signaling and general and vesicle related transport accounted for approximately 50% of the differences in both PM and DRM, indicating that from a qualitative point of view changes induced by Fe deficiency are similar in both preparations. Results indicate that Fe deficiency has an impact in phosphorylation processes at the PM level and highlight the involvement of signaling proteins, especially those from the 14-3-3 family. Lipid profiling revealed Fe-deficiency induced decreases in phosphatidic acid derivatives, which may impair vesicle formation, in agreement with the decreases measured in proteins related to intracellular trafficking and secretion. The modifications induced by Fe deficiency in the relative enrichment of proteins in DRMs revealed the existence of a group of cytoplasmic proteins that appears to be more attached to the PM in conditions of Fe deficiency.
  AMER CHEMICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1021/acs.jproteome.6b00026
  DOI ID：10.1021/acs.jproteome.6b00026, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：27321140, Web of Science ID：WOS:000381235900015
• Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Hiroyuki Imai; Yukio Kawamura; Matsuo Uemura
  CRYOBIOLOGY, Volume：72, Number：2, First page：123, Last page：134, Apr. 2016, [Reviewed], [Lead]
  Cold acclimation (CA) results in alteration of the plasma membrane (PM) lipid composition in plants, which plays a crucial role in the acquisition of freezing tolerance via membrane stabilization. Recent studies have indicated that PM structure is consistent with the fluid mosaic model but is laterally non homogenous and contains microdomains enriched in sterols, sphingolipids and specific proteins. In plant cells, the function of these microdomains in relation to CA and freezing tolerance is not yet fully understood. The present study aimed to investigate the lipid compositions of detergent resistant fractions of the PM (DRM) which are considered to represent microdomains. They were prepared from leaves of low-freezing tolerant oat and high-freezing tolerant rye. The DRMs contained higher proportions of sterols, sphingolipids and saturated phospholipids than the PM. In particular, one of the sterol lipid classes, acylated sterylglycoside, was the predominant sterol in oat DRM while rye DRM contained free sterol as the major sterol. Oat and rye showed different patterns (or changes) of sterols and 2-hydroxy fatty acids of sphingolipids of DRM lipids during CA. Taken together, these results suggest that CA induced changes of lipid classes and molecular species in DRMs are associated with changes in the thermodynamic properties and physiological functions of microdomains during CA and hence, influence plant freezing tolerance. (C) 2016 Elsevier Inc. All rights reserved.
  ACADEMIC PRESS INC ELSEVIER SCIENCE, English, Scientific journal
  DOI：https://doi.org/10.1016/j.cryobiol.2016.02.003
  DOI ID：10.1016/j.cryobiol.2016.02.003, ISSN：0011-2240, eISSN：1090-2392, PubMed ID：26904981, Web of Science ID：WOS:000374078600008
• Proteins Associated with Oxidative Burst and Cell Wall Strengthening Accumulate During Citrus-Xanthomonas Non-Host Interaction　　　　　　　　　　　　　　　
  T. Swaroopa Rani; Daisuke Takahashi; Matsuo Uemura; Appa Rao Podile
  PLANT MOLECULAR BIOLOGY REPORTER, Volume：33, Number：5, First page：1349, Last page：1360, Oct. 2015, [Reviewed]
  Citrus proteome changes at 8 and 48 h post inoculation (hpi) were analysed by both 2D gel electrophoresis and nano-LC-MS/MS proteomic approaches during interaction with Xanthomonas axonopodis pv. citri (Xac) and Xanthomonas oryzae pv. oryzae (Xoo) as host and non-host pathogens, respectively. A total of 256 proteins, 72 at 8 hpi and 184 at 48 hpi, differentially accumulated during citrus-Xanthomonas interaction. Of these, 67 and 115 proteins were specific to Xac and Xoo interaction, respectively. In addition, 64 proteins, 10 at 8 hpi and 54 at 48 hpi, variedly accumulated during both the interactions. Proteins related to photosynthesis, carbohydrate metabolism and protein synthesis were in low abundance during both the interactions resulting in reduced rate of photosynthesis. Proteins related to defence response, cell wall (CW) strengthening, lignin deposition and generation of reactive oxygen species (ROS) were in high abundance only during Xoo interaction. Whereas, during Xac interaction, proteins involved in antioxidant metabolism and CW loosening and/or elongation were in high abundance. The precise increase in abundance of these proteins during non-host interaction suggested an important role for CW fortification and ROS accumulation in non-host resistance in plants.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s11105-014-0817-y
  DOI ID：10.1007/s11105-014-0817-y, ISSN：0735-9640, eISSN：1572-9818, SCOPUS ID：84914128960, Web of Science ID：WOS:000360392800014
• The Distinct Functional Roles of the Inner and Outer Chloroplast Envelope of Pea (Pisum sativum) As Revealed by Proteomic Approaches　　　　　　　　　　　　　　　
  Elain Gutierrez-Carbonell; Daisuke Takahashi; Giuseppe Lattanzio; Jorge Rodriguez-Celma; Julia Kehr; Juergen Soll; Katrin Philippar; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOME RESEARCH, Volume：13, Number：6, First page：2941, Last page：2953, Jun. 2014, [Reviewed]
  Protein profiles of inner (IE) and outer (OE) chloroplast envelope membrane preparations from pea were studied using shotgun nLC-MS/MS and two-dimensional electrophoresis, and 589 protein species (NCBI entries) were identified. The relative enrichment of each protein in the IE/OE pair of membranes was used to provide an integrated picture of the chloroplast envelope. From the 546 proteins identified with shotgun, 321 showed a significant differential distribution, with 180 being enriched in IE and 141 in OE. To avoid redundancy and facilitate in silica localization, Arabidopsis homologues were used to obtain a nonredundant list of 409 envelope proteins, with many showing significant OE or IE enrichment. Functional classification reveals that IE is a selective barrier for transport of many metabolites and plays a major role in controlling protein homeostasis, whereas proteins in OE are more heterogeneous and participate in a wide range of processes. Data support that metabolic processes previously described to occur in the envelope such as chlorophyll and tocopherol biosynthesis can be ascribed to the IE, whereas others such as carotenoid or lipid biosynthesis occur in both membranes. Furthermore, results allow empirical assignation to the IE and/or OE of many proteins previously assigned to the bulk chloroplast envelope proteome.
  AMER CHEMICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1021/pr500106s
  DOI ID：10.1021/pr500106s, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：24792535, Web of Science ID：WOS:000337074500022
• Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins　　　　　　　　　　　　　　　
  Daisuke Takahashi
  Methods in Molecular Biology, Volume：1166, First page：159, Last page：170, May 2014, [Reviewed], [Lead]
  DOI：https://doi.org/10.1007/978-1-4939-0844-8_13
  DOI ID：10.1007/978-1-4939-0844-8_13, ISSN：1064-3745, ORCID：78380684, PubMed ID：24852635
• Temporal Analysis of Plasma Membrane Proteome in Arabidopsis during Cold Acclimation and Deacclimation　　　　　　　　　　　　　　　
  MIKI YUUSHI; TAKAHASHI DAISUKE; KAWAMURA YUKIO; UEMURA MATSUO
  低温生物工学会誌, Volume：60, Number：1, First page：35, Last page：41, Apr. 2014, [Reviewed]
  Overwintering temperate plants can enhance freezing tolerance in response to low, non-freezing temperatures (cold acclimation, CA). When cold-acclimated plants are transferred to a warm temperature, they reversibly lose their freezing tolerance rather quickly (deacclimation, DA). Particularly, DA is important under oscillatory temperature conditions in early spring in the field but has not been studied in detail. Thus, the purpose of this study is to accumulate fundamental knowledge of DA to explore possibilities to reduce freezing injury occurring in the DA period. First, we verified changes of freezing tolerance in Arabidopsis thaliana during CA and DA periods: acquired freezing tolerance after 1 week of CA at 2℃ was lost after 2 days of DA. Next, we focused on proteomic changes of the plasma membrane that is considered to be the primary site for determination of freezing tolerance. Proteome analysis identified 647 CA and/or DA responsive-proteins with different patterns of alternations during CA and DA periods and these proteins were categorized into specific functional categories. With these results, we discussed the function of CA and DA responsive-proteins in detail.
  Japanese Society for Cryobiology and Cryotechnology, Japanese
  ISSN：1340-7902, J-Global ID：201402271206507220, CiNii Articles ID：110009810731, CiNii Books ID：AN10448734
• Compositional Changes in Soluble Proteins of Tree Buds During Endodormancy Release　　　　　　　　　　　　　　　
  SUZUKI Shingo; TAKAHASHI Daisuke; KUWABARA Chikako; UEMURA Matsuo; ARAKAWA Keita
  Cryobiology and Cryotechnology, Volume：60, Number：1, First page：59, Last page：63, 2014, [Reviewed]
  In this study, we analyzed soluble proteins in winter bud during endodormancy release. Endodormancy of winter buds of Salix bakko and Larix kaempferi was released clearly in early and late December, respectively. SDS-PAGE analysis showed that soluble protein composition of buds in each species hardly changed in a short period of endodormancy release. Proteome analysis using LC-MS/MS revealed compositional changes in soluble proteins in this period of endodormancy release. However, the proportion of soluble proteins whose levels were changed in this period was under 5% of all detected proteins in each species of winter buds, and the variation was small.
  Japanese Society of Cryobiology and Cryotechnology, Japanese
  DOI：https://doi.org/10.20585/cryobolcryotechnol.60.1_59
  DOI ID：10.20585/cryobolcryotechnol.60.1_59, ISSN：1340-7902, J-Global ID：201402272670661654, CiNii Articles ID：110009810735, CiNii Books ID：AN10448734
• Shotgun proteomics of plant plasma membrane and microdomain proteins using Nano-LC-MS/MS　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1072, First page：481, Last page：498, 2014, [Reviewed], [Lead]
  Shotgun proteomics allows the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. In order to get comprehensive proteome profiles of the plasma membrane including highly hydrophobic proteins with a number of transmembrane domains, a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for proteins from the plasma membrane proteins and plasma membrane microdomain fraction is described. The results obtained are easily applicable to label-free protein semiquantification. © 2014 Springer Science+Business Media, LLC.
  Humana Press Inc., English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-62703-631-3_33
  DOI ID：10.1007/978-1-62703-631-3_33, ISSN：1064-3745, PubMed ID：24136542, SCOPUS ID：84934439028
• Changes of Detergent-Resistant Plasma Membrane Proteins in Oat and Rye during Cold Acclimation: Association with Differential Freezing Tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  JOURNAL OF PROTEOME RESEARCH, Volume：12, Number：11, First page：4998, Last page：5011, Nov. 2013, [Reviewed], [Lead]
  Cold acclimation (CA) results in an increase in freezing tolerance of plants, which is closely associated to functional changes of the plasma membrane (PM). Although proteomic studies have revealed compositional changes of the PM during CA, there has been no large-scale study of how the microdomains in the PM, which contains specific lipids and proteins, change during CA. Therefore, we conducted semi-quantitative shotgun proteomics using microdomain-enriched detergent-resistant membrane (DRM) fractions extracted from low freezing-tolerant oat and highly freezing-tolerant rye. We identified 740 and 809 DRM proteins in oat and rye, respectively. Among the proteins identified, the abundances of a variety of proteins, such as P-type ATPase and aquaporins, were affected by CA in both oat and rye. Some CA-responsive proteins in the DRM fractions, such as heat shock protein 70, changed differently in oat and rye. In addition, changes in lipocalins and sugar transporters in the DRM fractions were different from those found in total PM fraction during CA. This is the first report to describe compositional changes in the DRM during CA. The proteomic profiles obtained in the present study hint at many possible microdomain functions associated with CA and freezing tolerance.
  AMER CHEMICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1021/pr400750g
  DOI ID：10.1021/pr400750g, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：24111712, Web of Science ID：WOS:000326615000026
• Plant plasma membrane proteomics for improving cold tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  FRONTIERS IN PLANT SCIENCE, Volume：4, First page：90, Apr. 2013, [Reviewed], [Lead]
  Plants are always exposed to various stresses. We have focused on freezing stress, which causes serious problems for agricultural management. When plants suffer freeze-induced damage, the plasma membrane is thought to be the primary site of injury because of its central role in regulation of various cellular processes. Cold tolerant species, however, adapt to such freezing conditions by modifying cellular components and functions (cold acclimation). One of the most important adaptation mechanisms to freezing is alteration of plasma membrane compositions and functions. Advanced proteomic technologies have succeeded in identification of many candidates that may play roles in adaptation of the plasma membrane to freezing stress. Proteomics results suggest that adaptations of plasma membrane functions to low temperature are associated with alterations of protein compositions during cold acclimation. Some of proteins identified by proteomic approaches have been verified their functional roles in freezing tolerance mechanisms further. Thus, accumulation of proteomic results in the plasma membrane is of importance for application to molecular breeding efforts to increase cold tolerance in crops.
  FRONTIERS RESEARCH FOUNDATION, English
  DOI：https://doi.org/10.3389/fpls.2013.00090
  DOI ID：10.3389/fpls.2013.00090, ISSN：1664-462X, PubMed ID：23616787, Web of Science ID：WOS:000329825800001
• Compositional changes of plasma membrane proteins of Brachypodium distachyon during cold acclimation　　　　　　　　　　　　　　　
  NAKAYAMA TAKATO; TAKAHASHI DAISUKE; KAWAMURA YUKIO; RAHMAN ABIDUR; UEMURA MATSUO
  低温生物工学会誌, Volume：59, Number：1, First page：61, Last page：65, Apr. 2013, [Reviewed]
  To survive at freezing temperatures, temperate plants increase their freezing tolerance by cold acclimation, during which changes of protein and lipid compositions of the plasma membrane (PM) play a key role. Brachypodium distachyon, a temperate grass species, has been recognized as a model plant and is evolutionarily closer to temperate cereals than rice. These facts suggest that B. distachyon could serve as a good system to study freezing tolerance mechanism of temperate cereals. In the present study, using non-acclimated (NA) and cold-acclimated (CA, 2℃) B. distachyon, we performed freezing tolerance test and PM proteome analysis. When freezing tolerance was evaluated by measurement of electrolyte leakage, the temperature at which 50% electrolyte leakage occurs after freezing (T_) was lowered from -3.9℃ to -6.9℃ by cold acclimation. Furthermore based on the proteome analysis, PM proteins were found to be changed considerably by cold acclimation and the changes were similar in some parts to those found in oat and rye during cold acclimation. These results suggest that B. distachyon has an ability of cold acclimation and some common pathways responding to cold as shown in temperate cereals.
  Japanese Society for Cryobiology and Cryotechnology, Japanese
  ISSN：1340-7902, J-Global ID：201302245157841545, CiNii Articles ID：110009612769, CiNii Books ID：AN10448734
• Detergent-resistant plasma membrane proteome to elucidate microdomain functions in plant cells　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Frontiers in Plant Science, Volume：4, First page：27, Feb. 2013, [Reviewed], [Lead]
  Although proteins and lipids have been assumed to be distributed homogeneously in the plasma membrane (PM), recent studies suggest that the PM is in fact non-uniform structure that includes a number of lateral domains enriched in specific components (i.e., sterols, sphingolipids, and some kind of proteins). These domains are called as microdomains and considered to be the platform of biochemical reaction center for various physiological processes. Microdomain is able to be extracted as detergent-resistant membrane (DRM) fractions, and DRM fractions isolated from some plant species have been used for proteome and other biochemical characterizations to understand microdomain functions. Profiling of sterol-dependent proteins using a putative microdomain-disrupting agent suggests specific lipid-protein interactions in the microdomain. Furthermore, DRM proteomes dynamically respond to biotic and abiotic stresses in some plant species. Taken together, these results suggest that DRM proteomic studies provide us important information to understand physiological functions of microdomains that are critical to prosecute plant's life cycle successfully in the aspect of development and stress responses. © 2013 Takahashi, Kawamura and Uemura.
  Frontiers Research Foundation, English
  DOI：https://doi.org/10.3389/fpls.2013.00027
  DOI ID：10.3389/fpls.2013.00027, ISSN：1664-462X, PubMed ID：23440896, SCOPUS ID：84900836694
• 樹木冬芽の越冬過程における可溶性タンパク質の組成変化　　　　　　　　　　　　　　　
  鈴木伸吾; 遠藤圭太; 岡田香織; 荒川圭太; 高橋大輔; 上村松生
  日本木材学会北海道支部講演集(Web), Number：44, First page：WEB ONLY P-2, Nov. 2012, [Reviewed]
  Japanese
  J-Global ID：201202266951323958
• Function of Arabidopsis dynamin-related protein 1E during cold acclimation　　　　　　　　　　　　　　　
  KONDO MARIKO; TAKAHASHI DAISUKE; MINAMI ANZU; KAWAMURA YUKIO; UEMURA MATSUO
  低温生物工学会誌, Volume：58, Number：1, First page：105, Last page：110, Apr. 2012, [Reviewed]
  Japanese
  ISSN：1340-7902, J-Global ID：201202212052041990
• Detergent-resistant Plasma Membrane Proteome in Oat and Rye: Similarities and Dissimilarities between Two Monocotyledonous Plants　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Tetsuro Yamashita; Matsuo Uemura
  JOURNAL OF PROTEOME RESEARCH, Volume：11, Number：3, First page：1654, Last page：1665, Mar. 2012, [Reviewed], [Lead]
  The plasma membrane (PM) is involved in important cellular processes that determine the growth, development, differentiation, and environmental signal responses of plant cells. Some of these dynamic reactions occur in specific domains in the PM. In this study, we performed comparable nano-LC-MS/MS-based large-scale proteomic analysis of detergent-resistant membrane (DRM) fractions prepared from the PM of oat and rye. A number of proteins showed differential accumulation between the PM and DRM, and some proteins were only found in the DRM. Numerous proteins were identified as DRM proteins in oat (219 proteins) and rye (213 proteins), of which about half were identified only in the DRM. The DRM proteins were largely common to those found in dicotyledonous plants (Arabidopsis and tobacco), which suggests common functions associated with the DRM in plants. Combination of semiquantitative proteomic analysis and prediction of post-translational protein modification sites revealed differences in several proteins associated with the DRM in oat and rye. It is concluded that protein distribution in the DRM is unique from that in the PM, partly because of the physicochemical properties of the proteins, and the unique distribution of these proteins may define the functions of the specific domains in the PM in various physiological processes in plant cells.
  AMER CHEMICAL SOC, English, Scientific journal
  DOI：https://doi.org/10.1021/pr200849v
  DOI ID：10.1021/pr200849v, ISSN：1535-3893, PubMed ID：22191623, Web of Science ID：WOS:000300916200020
• Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line) after Cold and ABA Treatment in Association with Freezing Tolerance Development　　　　　　　　　　　　　　　
  Bin Li; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  PLANT AND CELL PHYSIOLOGY, Volume：53, Number：3, First page：543, Last page：554, Mar. 2012, [Reviewed]
  The plasma membrane (PM) is the primary site of freezing injury in plants. To determine global changes in PM protein profiles in association with freezing tolerance development, proteome analysis of the purified PM of Arabidopsis suspension-cultured cells (T87 line) was conducted with label-free protein quantification technology. Freezing tolerance of Arabidopsis cells at the lag growth phase (8 d old) increased after cold acclimation (CA) or ABA treatment. Proteome analysis assigned 658 proteins in the PM in total, of which 45.3% (298 proteins) were predicted to have transmembrane domains. They were classified into several functional categories, with the primary categories being proteins in transporters, signal transduction, protein destination and storage, and cell structure. After CA, 271 proteins increased and 111 proteins decreased. ABA treatment resulted in 185 increased and 56 decreased proteins. Of these, 139 increased and 49 decreased proteins were identified in common after both CA and ABA treatment. In addition, there were proteins specifically expressed in cold- (132 increased and 62 decreased) or ABA- (46 increased and 7 decreased) treated cells. Collectively, our results clearly show that (i) responses of the PM proteome to CA and ABA treatment overlap substantially but, at the same time, some proteins exhibited different response patterns in each treatment; and (ii) the majority of ABA-responsive proteins are CA-responsive proteins but not vice versa, suggesting complex interactions of CA and ABA signaling pathways in the PM proteome responses.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcs010
  DOI ID：10.1093/pcp/pcs010, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：22318864, Web of Science ID：WOS:000301359500005
• Relationship of Plasma Membrane Microdomain Composition and Freezing Tolerance in Oat and Rye　　　　　　　　　　　　　　　
  TAKAHASHI DAISUKE; FURUTO AKARI; MINAMI ANZU; KAMO MASAHARU; YAMASHITA TETSURO; UEMURA MATSUO
  低温生物工学会誌, Volume：57, Number：1, First page：95, Last page：99, Apr. 2011, [Reviewed], [Lead]
  Japanese
  ISSN：1340-7902, J-Global ID：201102251181376530

• フルクタンの蓄積は気温の昼夜変化条件における凍結耐性の変化に関係している　　　　　　　　　　　　　　　
  菊地拓真; Sushan Chowhan; 小竹敬久; 高橋大輔
  Mar. 2025
  Mar. 2025 - Mar. 2025, Japanese, Poster presentation
• G 層形成時に発現誘導が認められた糖転移酵素の機能解析　　　　　　　　　　　　　　　
  福榮美月; 玉野桜子; 高橋大輔; 小竹敬久; 家門絵理; 石川和也; 石水毅
  Mar. 2025
  Mar. 2025 - Mar. 2025, Japanese, Oral presentation
• 乾燥耐性の異なる2 種の Selaginella 属植物の細胞壁構造　　　　　　　　　　　　　　　
  大橋桃花; 菊地拓真; 厚沢季美江; 金子康子; 小竹敬久; 高橋大輔
  Mar. 2025
  Mar. 2025 - Mar. 2025, Japanese, Oral presentation
• ペクチンのアラビナンおよびガラクタンは花粉の形成に関わる　　　　　　　　　　　　　　　
  菊地拓真; 曽我康一; 小竹敬久; 高橋大輔
  Oct. 2024
  Oct. 2024 - Oct. 2024, Japanese, Oral presentation
• Adaptation mechanism of plants to freezing stress - from the viewpoint of the cell wall　　　　　　　　　　　　　　　
  Daisuke Takahashi
  RIKEN CSRS Seminar, Oct. 2024, [Invited]
  Oct. 2024 - Oct. 2024, English, Public discourse
• GDP-マンノース合成制御因子KONJAC1の植物特有の性状と役割　　　　　　　　　　　　　　　
  本田零; 宮川萌; 高橋大輔; 小竹敬久
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
• チャの高アルミニウム集積能と細胞壁コンポーネントの関係　　　　　　　　　　　　　　　
  朝比奈美緒; 山下寛人; 米澤詩織; 菊池拓真; 高橋大輔; 廣野裕平; 一家崇志
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
• 根毛の細胞成長方向の維持における細胞壁内の記憶　　　　　　　　　　　　　　　
  四方 明格; 高橋 大輔; 小竹 敬久
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Public symposium
• 生殖発生過程におけるペクチンのアラビナンおよびガラクタン側鎖の相補的な関わり　　　　　　　　　　　　　　　
  菊地拓真; 曽我康一; 小竹敬久; 高橋大輔
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
• Roles of soluble sugars and cell wall polysaccharides for acquisition of freezing tolerance in wheat　　　　　　　　　　　　　　　
  Chowhan Sushan; 久津野達也; 半田寛斗; 小竹敬久; 高橋大輔
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
• チャのアルミニウム高集積能に寄与する細胞壁コンポーネントの評価　　　　　　　　　　　　　　　
  朝比奈美緒; 山下寛人; 米澤詩織; 高橋大輔; 廣野祐平; 一家崇志
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
• Both trehalose and cell wall polysaccharides may be involved in the desiccation tolerance exhibited by Selaginella tamariscina.　　　　　　　　　　　　　　　
  Kikuchi T; Ohashi M; Kaneko Y; Kotake T; Takahashi D
  13th International Plant Cold Hardiness Seminar, Aug. 2024
  Aug. 2024 - Aug. 2024, English, Oral presentation
• Xyloglucan is associated with basal freezing tolerance and growth plasticity　　　　　　　　　　　　　　　
  Kutsuno T; Soga K; Kotake T; Takahashi D
  8th International Conference on Plant Cell Wall Biology, Jun. 2024
  Jun. 2024 - Jun. 2024, English, Oral presentation
• Functions of atypical endo-β-1,4-mannanases in the synthesis and deposition of glucomannan　　　　　　　　　　　　　　　
  Kikuch Ai; Nishigaki N; Takasaki H; Takahashi D; Kotake T
  8th International Conference on Plant Cell Wall Biology, Jun. 2024
  Jun. 2024 - Jun. 2024, English, Oral presentation
• Selaginella属植物の高い乾燥耐性に寄与する細胞壁関連メカニズムの解明　　　　　　　　　　　　　　　
  大橋桃花; 菊地拓真; 辻季美江; 金子康子; 小竹敬久; 高橋大輔
  Jun. 2024
  Jun. 2024 - Jun. 2024, Japanese, Oral presentation
• キバナノセッコクにみられるグルコマンナンの液胞蓄積　　　　　　　　　　　　　　　
  菊地愛菜; 西垣南歩; 厚沢季美江; 小野真央; Chuan-Ming Yeh; Wen-Chieh Tsai; 石水毅; 金子康子; 高橋大輔; 小竹敬久
  Mar. 2024
  Mar. 2024 - Mar. 2024, Japanese, Oral presentation
• 低温馴化および脱馴化における細胞壁変化は凍結耐性と生長に関わる　　　　　　　　　　　　　　　
  久津野達也; 小竹敬久; 高橋大輔
  Mar. 2024
  Mar. 2024 - Mar. 2024, Japanese, Oral presentation
• イワヒバが示す乾燥耐性は，トレハロースよりも細胞壁に起因する可能性がある　　　　　　　　　　　　　　　
  菊地拓真; 大橋桃花; 金子康子; 小竹敬久; 高橋大輔
  Mar. 2024
  Mar. 2024 - Mar. 2024, Japanese, Oral presentation
• Changes in soluble sugars and cell wall in wheat cultivars during cold acclimation.　　　　　　　　　　　　　　　
  Chowhan S; Kotake T; Takahashi D
  細胞壁研究者ネットワーク第17回定例研究会, Oct. 2023
  Oct. 2023 - Oct. 2023, English, Oral presentation
• Complementary effects of pectic arabinan and galactan on pollen development.　　　　　　　　　　　　　　　
  Kikuchi T; Soga K; Kotake T; Takahashi D
  Taiwan-Japan Plant Biology, Oct. 2023
  Oct. 2023 - Oct. 2023, English, Poster presentation
• Cell wall composition may explain desiccation tolerance in Selaginella tamariscina.　　　　　　　　　　　　　　　
  Kikuchi T; Ohashi M; Kaneko Y; Kotake T; Takahashi D
  Taiwan-Japan Plant Biology, Oct. 2023
  Oct. 2023 - Oct. 2023, English, Poster presentation
• Phylogenetic distribution of UDP-glucose 4-epimerases with UDP-xylose 4-epimerase activity in plants.　　　　　　　　　　　　　　　
  Handa H; Matsumoto M; Miyagawa M; Takahashi D; Fushinobu S; Kotake T
  Taiwan-Japan Plant Biology, Oct. 2023
  Oct. 2023 - Oct. 2023, English, Poster presentation
• Spatiotemporal control of ice crystallization in supercooled water by focused irradiation with laser pulses.　　　　　　　　　　　　　　　
  Takahashi H; Kono T; Sawada K; Kumano S; Tsuri Y; Maruyama M; Yoshimura M; Takahashi D; Kawamura Y; Uemura M; Nakabayashi S; Mori Y; Hosokawa Y; Yoshikawa HY
  International Conference on Crystal Growth and Epitaxy, Jul. 2023
  Jul. 2023 - Aug. 2023, English, Oral presentation
• Adaptation mechanism of plants to freezing stress - from the viewpoint of cell wall　　　　　　　　　　　　　　　
  Takahashi D
  Research seminar at Jagiellonian University, Jun. 2023, [Invited]
  Jun. 2023 - Jun. 2023, English, Public discourse
• Structural differences between membrane-bound and apoplastic arabinogalactan-proteins (AGPs) and their response to low temperature　　　　　　　　　　　　　　　
  Numao Y; Tsumuraya Y; Humphries J; Johnson K; Bacic A; Kotake T; Takahashi D
  XVI Plant Cell Wall Meeting, Jun. 2023
  Jun. 2023 - Jun. 2023, English, Poster presentation
• Studies on the response of plasma membrane and cell wall to low temperatures in plants　　　　　　　　　　　　　　　
  Takahashi D
  第68回低温生物工学会, Jun. 2023
  Jun. 2023 - Jun. 2023, Japanese, Invited oral presentation
• ペクチン側鎖のガラクタンおよびアラビナンが植物の低温応答に及ぼす影響　　　　　　　　　　　　　　　
  菊地拓真; 曽我康一; 小竹敬久; 高橋大輔
  Jun. 2023
  Jun. 2023 - Jun. 2023, Japanese, Oral presentation
• シロイヌナズナナチュラルアクセッションにおける細胞壁の低温応答性　　　　　　　　　　　　　　　
  菊地由華; 小竹敬久; 高橋大輔
  Jun. 2023
  Jun. 2023 - Jun. 2023, Japanese, Oral presentation
• High-speed observation of ice crystallization dynamics in supercooled water triggered by laser ablation.　　　　　　　　　　　　　　　
  Takahashi H; Kono T; Sawada K; Kumano S; Tsuri Y; Maruyama M; Yoshimura M; Takahashi D; Kawamura Y; Uemura M; Nakabayashi S; Mori Y; Hosokawa Y; Yoshikawa HY
  The 24th International Symposium on Laser Precision Microfabrication, Jun. 2023
  Jun. 2023 - Jun. 2023, English, Oral presentation
• Changes in soluble sugars and cell wall in wheat cultivars during cold acclimation.　　　　　　　　　　　　　　　
  Chowhan S; Kutsuno T; Handa H; Kotake T; Takahashi D
  Mar. 2023
  Mar. 2023 - Mar. 2023, English, Poster presentation
• Function of KONAJC1 protein in the synthesis of L-ascorbic acid.　　　　　　　　　　　　　　　
  Miyagawa M; Takahashi D; Kotake T
  Mar. 2023
  Mar. 2023 - Mar. 2023, Japanese, Poster presentation
• Cell wall changes during cold and deacclimation in association with freezing tolerance and growth　　　　　　　　　　　　　　　
  Takahashi D; Sasaki K; Kutsuno T; Kikuchi T; Soga K; Kotake T
  International Symposium on “Plant-Structure Optimization”, Nov. 2022, [Invited]
  Nov. 2022 - Nov. 2022, English, Invited oral presentation
• ペクチンのアラビナン側鎖の細胞接着における役割.　　　　　　　　　　　　　　　
  樋口慶太; 曽我康一; 金子康子; 九鬼寛明; 高橋大輔; 小竹敬久
  Sep. 2022
  Sep. 2022 - Sep. 2022, Japanese, Oral presentation
• デンドロビウムのグルコマンナンのアセチル化に関するTBLファミリータンパク質.　　　　　　　　　　　　　　　
  菊地愛菜; 西垣南歩; 小野真央; 石水毅; Tsai Wen-Chieh; Yeh Chuan-Ming; 高橋大輔; 小竹敬久
  Sep. 2022
  Sep. 2022 - Sep. 2022, Japanese, Oral presentation
• シロイヌナズナの低温馴化および脱馴化過程における細胞壁変化の統合的解析　　　　　　　　　　　　　　　
  久津野達也; 小竹敬久; 高橋大輔
  Jun. 2022
  Jun. 2022 - Jun. 2022, Japanese, Oral presentation
• Acetylated glucomannan in Dendrobium catenatum　　　　　　　　　　　　　　　
  Naho Nishigaki; Aina Kikuchi; Mao Ono; Daisuke Takahashi; Takeshi Ishimizu; Toshihisa Kotake
  IX Cell Wall Research Conference, Jun. 2022
  Jun. 2022 - Jun. 2022, English, Poster presentation
• Cold acclimation process induces accumulation of pectic galactan, which enhances freezing tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kazuma Sasaki; Tatsuya Kutsuno; Kim Johnson; Arun Sampathkumar; Antony Bacic; Ellen Zuther; Kotake Toshihisa
  IX Cell Wall Research Conference, Jun. 2022
  Jun. 2022 - Jun. 2022, English, Oral presentation
• Contribution of galactan to the mechanism of plant adaptation to freezing　　　　　　　　　　　　　　　
  Daisuke Takahashi
  Scandinavian Plant Physiology Society&Physiologia Plantarum Webinar series “Cold Tolerance & Adaptation”, May 2022, [Invited]
  May 2022 - May 2022, English, Nominated symposium
• 植物の凍結適応メカニズム：細胞壁の観点から　　　　　　　　　　　　　　　
  高橋大輔
  Mar. 2022, [Invited]
  Mar. 2022 - Mar. 2022, Japanese, Nominated symposium
• The function of FLA8 in cold acclimation and freezing tolerance acquisition mechanism　　　　　　　　　　　　　　　
  Yukino Shibasaki; Kim Johnson; Antony Bacic; Toshihisa Kotake; Daisuke Takahashi
  Mar. 2022
  Mar. 2022 - Mar. 2022, Japanese, Oral presentation
• A cell wall pectic galactan is involved in the mechanism of freezing tolerance acquisition during cold acclimation　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kazuma Sasaki; Tatsuya Kutsuno; Kim Johnson; Arun Sampathkumar; Antony Bacic; Ellen Zuther; Kotake Toshihisa
  12th International Plant Cold Hardiness Seminar, Dec. 2021
  Dec. 2021 - Dec. 2021, English, Oral presentation
• グルコマンナンの修飾に関わるランの遺伝子の機能解析　　　　　　　　　　　　　　　
  西垣南歩; Chuan-Ming Yeh; Wen-Chieh Tsai; 円谷陽一; 高橋大輔; 小竹敬久
  Sep. 2021
  Sep. 2021 - Sep. 2021, Japanese, Oral presentation
• KONJAC1 はVTC1 の分解を抑制することでL- アスコルビン酸含量に影響する　　　　　　　　　　　　　　　
  宮川萌; 高橋大輔; 小竹敬久
  Sep. 2021
  Sep. 2021 - Sep. 2021, Japanese, Oral presentation
• 膜結合型及び遊離型AGP の構造と低温応答性　　　　　　　　　　　　　　　
  沼尾悠太; 円谷陽一; 小竹敬久; 高橋大輔
  Sep. 2021
  Sep. 2021 - Sep. 2021, Japanese, Oral presentation
• AGP のβ-1,6- ガラクタン側鎖のin vivo 分解　　　　　　　　　　　　　　　
  原克弥; 吉見圭永; 菊池愛菜; 円谷陽一; 高橋大輔; 小竹敬久
  Sep. 2021
  Sep. 2021 - Sep. 2021, Japanese, Oral presentation
• The role of pectin side chain in cold acclimation and their relationship to freezing adaptation in plants.　　　　　　　　　　　　　　　
  Takahashi D; Johnson K; Soga K; Sampathkumar A; Bacic A; Zuther E; Kotake T
  Plant Cell Wall Biology 2021, Jun. 2021
  Jun. 2021 - Jul. 2021, English, Oral presentation
• Preparation of AMOR using glycolytic enzymes.　　　　　　　　　　　　　　　
  Liu L; Tsumuraya Y; Takahashi D; Kotake T
  Plant Cell Wall Biology 2021, Jun. 2021
  Jun. 2021 - Jul. 2021, English, Poster presentation
• シロイヌナズナにおける二つ目のUDP-L-アラビノース合成経路　　　　　　　　　　　　　　　
  梅澤輝; 中澤小夏; 伏信進矢; 西垣南歩; 円谷陽一; 高橋大輔; 小竹敬久
  Mar. 2021
  Mar. 2021 - Mar. 2021, Japanese, Oral presentation
• Pleiotropic changes of arabinogalactan proteins during cold acclimation treatment.　　　　　　　　　　　　　　　
  Takahashi D; Numao Y; Shibasaki Y; Tsumuraya Y; Kotake T
  Mar. 2021
  Mar. 2021 - Mar. 2021, Japanese, Poster presentation
• 植物の低温応答と凍結耐性獲得機構ー細胞壁の観点からー　　　　　　　　　　　　　　　
  高橋大輔
  Dec. 2020, [Invited]
  Japanese, Nominated symposium
• 植物の凍結適応過程で起こる細胞壁変化　　　　　　　　　　　　　　　
  高橋大輔
  Nov. 2020, [Invited]
  Japanese, Nominated symposium
• 植物の低温・氷点下温度馴化過程で起こる細胞壁成分変化の解析.　　　　　　　　　　　　　　　
  高橋大輔; Kim Johnson; Antony Bacic; 曽我康一; Arun Sampathkumar; Ellen Zuther; 小竹敬久; Dirk K. Hincha
  Sep. 2020
  Japanese, Oral presentation
• シロイヌナズナmur1-1胚軸をモデルとした細胞壁の化学/物理学的特性の解析　　　　　　　　　　　　　　　
  九鬼寛明; 曽我康一; 西垣南歩; 竹中悠人; 山口雅利; 石水毅; 高橋大輔; 小竹敬久
  Sep. 2020
  Japanese, Oral presentation
• KONJAC タンパク質がビタミンC合成に与える影響　　　　　　　　　　　　　　　
  阿部桃太; 宮川萌; 西垣南歩; 山梨優貴子; 杢屋公介; 円谷陽一; 高橋大輔; 小竹 敬久
  Sep. 2020
• KONJACタンパク質のグルコマンナン合成における役割.　　　　　　　　　　　　　　　
  西垣南歩; 吉見圭永; 國枝正; 高橋大輔; 円谷陽一; 小竹敬久
  Sep. 2020
  Japanese, Oral presentation
• UDP-L-アラビノース合成系の起源と生理的意義の解明.　　　　　　　　　　　　　　　
  梅澤輝; 曳田優; 松村理奈; 西垣南歩; 高橋大輔; 円谷陽一; 小竹 敬久
  Sep. 2020
  Japanese, Oral presentation
• シロイヌナズナにおけるファシクリン様アラビノガラクタンタンパク質遺伝子の低温応答性　　　　　　　　　　　　　　　
  高橋大輔; 柴崎由季乃; 小竹敬久
  May 2020
  May 2020 - May 2020, Japanese, Oral presentation
• Diverse changes in cell wall structure and composition in response to cold and sub-zero acclimation.　　　　　　　　　　　　　　　
  Takahashi D; Sampathkumar A; Johnson K; Bacic A; Zuther E; Hincha DK
  Mar. 2020
  Japanese, Poster presentation
• Xyloglucan endotransglucosylase/hydrolase is involved in cold- and sub-zero acclimation-induced freezing tolerance in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Takahashi D; Sampathkumar A; Johnson K; Bacic A; Yokoyama R; Kuroha T; Nishitani K; Zuther E; Hincha DK
  理化学研究所-埼玉大学理工学研究科 第4回生命科学系合同シンポジウム, Nov. 2019, [Domestic conference]
  Japanese, Poster presentation
• Xyloglucan endotransglucosylase/hydrolase is involved in cold- and sub-zero acclimation-induced freezing tolerance in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Takahashi D; Sampathkumar A; Johnson K; Bacic A; Yokoyama R; Kuroha T; Nishitani K; Zuther E; Hincha DK
  GlycoTOKYO 2019, Nov. 2019, [Domestic conference]
  Japanese, Poster presentation
• 凍結ストレスに対する植物の応答機構〜細胞膜と細胞壁の観点から〜　　　　　　　　　　　　　　　
  高橋大輔
  Apr. 2019, [Invited], [Domestic conference]
  Japanese, Public discourse
• 凍結ストレスに対する植物の応答機構〜細胞膜と細胞壁の観点から〜　　　　　　　　　　　　　　　
  高橋大輔
  Mar. 2019, [Invited], [Domestic conference]
  Japanese, Public discourse
• 凍結ストレスに対する植物の応答機構〜細胞膜と細胞壁の観点から〜　　　　　　　　　　　　　　　
  高橋大輔
  Mar. 2019, [Invited], [Domestic conference]
  English, Public discourse
• A single seed treatment with hydroxyl radical/reactive oxygen species: a potential solution against emerging threat of multiple abiotic stresses.　　　　　　　　　　　　　　　
  Mostafa KM; Erazo C; Takahashi D; Tanino K; Kawamura Y; Uemura M
  Mar. 2019, [Domestic conference]
  English, Poster presentation
• Involvement of xyloglucan endotransglucosylase/hydrolase in plant freezing tolerance.　　　　　　　　　　　　　　　
  Takahashi D; Sampathkumar A; Yokoyama R; Kuroha T; Nishitani K; Zuther E; Hincha DK
  Mar. 2019, [Domestic conference]
  Japanese, Poster presentation
• 凍結ストレスに対する植物の応答機構〜細胞膜と細胞壁の観点から〜　　　　　　　　　　　　　　　
  高橋大輔
  Feb. 2019, [Invited], [Domestic conference]
  Japanese, Public discourse
• Shotgun proteomics of saposin D mutant mouse brain using nano-LC-MS/MS　　　　　　　　　　　　　　　
  Watanabe E; Yamashita T; Uemura M; Takahashi D; Go S; Otomo T; Matsuda J
  The 91st Annual Meeting of the Japanese Biochemical Society, Sep. 2018, [Domestic conference]
  Japanese, Poster presentation
• シロイヌナズナの根の水分屈性にMIZ1と共にHSP90とNPH3が機能する.　　　　　　　　　　　　　　　
  山崎 誠和; 小泉 日輪; 小林 啓恵; 藤井 伸治; 宮沢 豊; 高橋 大輔; 河村 幸男; 上村 松生; 高橋 秀幸
  Sep. 2018, [Domestic conference]
  Japanese, Poster presentation
• HSP90とNPH3がシロイヌナズナの根の水分屈性をMIZ1と協調的に制御する可能性　　　　　　　　　　　　　　　
  山崎 誠和; 小泉 日輪; 小林 啓恵; 藤井 伸治; 宮沢 豊; 高橋 大輔; 河村 幸男; 上村 松生; 高橋 秀幸
  Sep. 2018, [Domestic conference]
  Japanese, Oral presentation
• Molecular insights into changes of the extracellular matrix during cold and sub-zero acclimation.　　　　　　　　　　　　　　　
  Takahashi D; Sampathkumar A; Erban A; Gorka M; Kopka J; Graf A; Yokoyama R; Kuroha T; Nishitani K; Zuther E; Hincha DK
  11th International Plant Cold Hardiness Seminar, Aug. 2018, [International conference]
  English, Oral presentation
• Responses of the cell wall during cold and sub-zero acclimation in Arabidopsis: molecular and physiological implications for freezing tolerance　　　　　　　　　　　　　　　
  Takahashi D; Erban A; Gorka M; Sampathkumar A; Kopka J; Graf A; Zuther E; Hincha DK
  VIII. Cell Wall Research Conference, Jun. 2018, [International conference]
  English, Oral presentation
• Elucidation of physiological and molecular responses of the extracellular matrix of the higher plant Arabidopsis thaliana during cold and sub-zero acclimation　　　　　　　　　　　　　　　
  Takahashi D
  Network Meeting of the Alexander von Humboldt Foundation, Feb. 2018, [Domestic conference]
  English, Poster presentation
• 植物の耐凍性を最大化する～氷点下温度馴化～　　　　　　　　　　　　　　　
  高橋大輔
  Sep. 2017, [Invited], [Domestic conference]
  Japanese, Public discourse
• ドロノキ放射柔細胞の細胞死に関連するプロテアーゼの遺伝子発現解析　　　　　　　　　　　　　　　
  半智史; 高田直樹; 高橋大輔; 中田了五; 上村松生; 船田良
  Sep. 2017, [Domestic conference]
  Japanese, Poster presentation
• 細胞膜および細胞膜マイクロドメインの低温馴化応答性と凍結耐性　　　　　　　　　　　　　　　
  高橋大輔
  Sep. 2017, [Invited], [Domestic conference]
  Japanese, Invited oral presentation
• 植物の耐凍性を最大化する～氷点下温度馴化～　　　　　　　　　　　　　　　
  高橋大輔
  Sep. 2017, [Invited], [Domestic conference]
  Japanese, Public discourse
• Effects of Mn toxicity on the protein profiles of tomato (Solanum lycopersicum) xylem sap and roots.　　　　　　　　　　　　　　　
  Ceballos-Laita L; Gutierrez-Carbonell E; Takahashi D; Uemura M; Abadía A; Abadía J; López-Millán AF
  International Plant Nutrition Colloquium IPNC 2017, Aug. 2017, [International conference]
  English, Poster presentation
• Sub-zero acclimation induces enhancement of freezing tolerance accompanied by changes of extracellular proteome and cell wall characteristics in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Gorka M; Erban A; Graf A; Kopka J; Zuther E; Hincha DK
  International Proteomics Conference (IPC) 2017, Aug. 2017, [International conference]
  English, Oral presentation
• ドロノキ放射柔細胞の細胞死に関連するプロテアーゼの遺伝子発現解析　　　　　　　　　　　　　　　
  半智史; 高田直樹; 高橋大輔; 中田了五; 上村松生; 船田良
  Mar. 2017, [Domestic conference]
  Japanese, Oral presentation
• Impact of sub-zero acclimation on the extracellular matrix and its significance in freezing tolerance　　　　　　　　　　　　　　　
  Takahashi D; Erban A; Gorka M; Kopka J; Graf A; Zuther E; Hincha DK
  日本植物生理学会2016年度年会, Mar. 2017, [Domestic conference]
  English, Oral presentation
• Apoplastic proteome and biochemical responses to cold acclimation in ‘Norstar’ winter wheat crown tissues　　　　　　　　　　　　　　　
  Willick IR; Takahashi D; Uemura M; Fowler DB; Tanino KK
  日本植物生理学会2016年度年会, Mar. 2017, [Domestic conference]
  English, Oral presentation
• Proteomics approaches to elucidate the involvement of plasma membrane proteins in plant cold acclimation　　　　　　　　　　　　　　　
  Uemura M; Takahashi D; Nakayama T; Miki Y; Li B; Kondo M; Minami A; Yago K; Watanabe E; Kawamura Y
  1st UGAS, Iwate University International Symposium, Dec. 2016, [Invited], [International conference]
  English, Invited oral presentation
• Complex responses of glycosylphosphatidylinositol-anchored proteins in association with plant cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Kawamura Y; Uemura M
  3rd Meeting of Asia Oceania Agricultural Proteomics Organization, Dec. 2016, [Invited], [International conference]
  English, Invited oral presentation
• Investigation of proteins responsible for root hydrotropism, but not for gravitropism　　　　　　　　　　　　　　　
  Taguwa N; Kuya N; Takahashi D; Kawamura Y; Uemura M; Takahashi H; Miyazawa Y
  11th Asian Microgravity Symposium 2016, Oct. 2016, [International conference]
  English, Poster presentation
• Changes of extracellular matrix in response to cold and sub-zero acclimation in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Gorka M; Erban A; Graf A; Kopka J; Zuther E; Hincha DK
  2nd International Plant Proteomics Organization (INPPO) World Congress, Sep. 2016, [International conference]
  English, Oral presentation
• Effect of iron deficiency in the seed proteome of two Medicago truncatula ecotypes differing in mineral accumulation patterns　　　　　　　　　　　　　　　
  Ceballos-Laita L; García CC; Takahashi D; Uemura M; Abadía A; Grusak M; Abadía J; López-Millán AF
  18th ISINIP International Symposium on Iron Nutrition and Interactions in Plants, May 2016, [International conference]
  English, Poster presentation
• Effects of Fe and Mn deficiencies in the protein profiles of tomato (Solanum lycopersicum) xylem sap　　　　　　　　　　　　　　　
  Ceballos L; Takahashi D; Uemura M; Abadía A; Abadía J; López-Millán A-F
  18th ISINIP International Symposium on Iron Nutrition and Interactions in Plants, May 2016, [International conference]
  English, Oral presentation
• ドロノキ木部放射柔細胞の放射方向におけるタンパク質変動のショットガンプロテオーム解析　　　　　　　　　　　　　　　
  半智史; 高橋大輔; 梅澤泰史; 春日純; 高田直樹; 中田了五; 上村松生; 船田良
  Mar. 2016, [Domestic conference]
  Japanese, Oral presentation
• Proteomic changes in soluble and plasma membrane proteins during seasonal cold-deacclimation and acclimation processes in poplar twigs　　　　　　　　　　　　　　　
  Kasuga J; Kayano M; Takahashi D; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiologists, Mar. 2016, [Domestic conference]
  Japanese, Poster presentation
• Identification of proteins that interact with MIZU-KUSSEI2, a protein required for hydrotropism in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Taguwa N; Kuya N; Takahashi D; Kawamura Y; Uemura M; Takahashi H; Miyazawa Y
  The Japanese Society of Plant Physiologists, Mar. 2016, [Domestic conference]
  Japanese, Poster presentation
• Molecular responses of extracellular matrix to cold and sub-zero acclimation in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Gorka M; Erban A; Graf A; Kopka J; Zuther E; Hincha DK
  The Japanese Society of Plant Physiologists, Mar. 2016, [Domestic conference]
  Japanese, Poster presentation
• 植物の耐凍性を最大化する～氷点下温度馴化～(＋ドイツの研究環境を垣間見て)　　　　　　　　　　　　　　　
  高橋大輔
  Dec. 2015, [Invited], [Domestic conference]
  Japanese, Public discourse
• 細胞膜および細胞膜マイクロドメインの低温馴化応答性と凍結耐性　　　　　　　　　　　　　　　
  高橋大輔
  Dec. 2015, [Invited], [Domestic conference]
  Japanese, Invited oral presentation
• シロイヌナズナ水分屈性制御分子MIZ2と相互作用するタンパク質の機能解析　　　　　　　　　　　　　　　
  田桑菜津子; 久家徳之; 高橋大輔; 河村幸男; 上村松生; 高橋秀幸; 宮沢豊
  Dec. 2015, [Domestic conference]
  Japanese, Oral presentation
• 植物の凍結耐性獲得機構における細胞膜および細胞膜マイクロドメインの応答性　　　　　　　　　　　　　　　
  高橋大輔
  Dec. 2015, [Invited], [Domestic conference]
  Japanese, Public discourse
• Overcome freezing stress~survival strategies in plants ~　　　　　　　　　　　　　　　
  Takahashi D
  3rd Cross-disciplinary Meeting of Japanese Young Scientists in Europe, Aug. 2015, [International conference]
  Japanese, Oral presentation
• Changes in the protein profile of seeds from two genotypes of Medicago truncatula as affected by iron deficiency　　　　　　　　　　　　　　　
  Ceballos L; Garcia C; Takahashi D; Abadia A; Grusak MA; Uemura M; Abadia J; Lopez-Millan A-F
  Plant Proteomics Workshop, Jul. 2015, [International conference]
  English, Poster presentation
• GPI-anchored proteins in response to cold acclimation: from “Omics” to “Physiology”　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  Vienna International Conferences and Events Association "Plant Abiotic Stress Tolerance III", Jun. 2015, [International conference]
  English, Oral presentation
• The distinct functional roles of the inner and outer chloroplast envelope of pea (Pisum sativum) as revealed by proteomic approaches　　　　　　　　　　　　　　　
  Gutierrez-Carbonell E; Ceballos-Laita L; Takahashi D; Uemura M; Abadia A; Lopez-Millan A-F; Abadia J
  XIV Congreso Hispano-Luso de Fisiologia Vegetal, Jun. 2015, [Domestic conference]
  Spanish, Castilian, Oral presentation
• Advances in iron nutrition based on mass spectrometry approaches　　　　　　　　　　　　　　　
  Abadia J; Siso-Terraza P; Diaz-Benito P; Gutierrez-Carbonell E; Takahashi D; Abadia A; Uemura M; Lopez-Millan A-F; Alvarez-Fernandez A
  XVII International Symposium on Iron Nutrition and Interactions in Plants, Jun. 2015, [Invited], [International conference]
  English, Keynote oral presentation
• シロイヌナズナの低温初期応答に関するリン酸化プロテオミクス　　　　　　　　　　　　　　　
  三木雄史; 野村有子; 高橋大輔; 河村幸男; 中神弘史; 上村 松生
  Mar. 2015, [Domestic conference]
  Japanese, Oral presentation
• Analysis of GPI-anchored β-1,3-Glucanase　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiologists, Mar. 2015, [Domestic conference]
  Japanese, Oral presentation
• A putative GPI-anchored β-1,3-glucanase is involved in acquisition of freezing tolerance during cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  The 2nd International Symposium on Plant Environmental Sensing, Mar. 2015, [International conference]
  English, Poster presentation
• シロイヌナズナ水分屈性制御分子MIZ2 と相互作用するタンパク質の探索　　　　　　　　　　　　　　　
  田桑菜津子; 久家徳之; 高橋大輔; 河村幸男; 上村松生; 高橋秀幸; 宮沢 豊
  Dec. 2014, [Domestic conference]
  Japanese, Poster presentation
• 低温馴化および脱馴化シロイヌナズナにおける細胞膜タンパク質のリン酸化状態の解析　　　　　　　　　　　　　　　
  三木雄史; 野村有子; 高橋大輔; 河村幸男; 中神弘史; 上村松生
  Dec. 2014, [Domestic conference]
  Japanese, Oral presentation
• GPI-Proteome解析から得られた低温馴化関連タンパク質の凍結耐性獲得機構における機能　　　　　　　　　　　　　　　
  高橋大輔; 冨永陽子; 河村幸男; 上村松生
  Dec. 2014, [Domestic conference]
  Japanese, Oral presentation
• A cold acclimation-responsive glycosylphosphatidylinositol-anchored protein (GPI-AP) is necessary for the acquisition of freezing tolerance in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  Present Forestry, Agriculture and Husbandry Conditions in Mongolia, Dec. 2014, [International conference]
  English, Oral presentation
• 低温馴化応答性GPIアンカー型タンパク質の凍結耐性との関連　　　　　　　　　　　　　　　
  高橋大輔
  Oct. 2014, [Domestic conference]
  Japanese, Oral presentation
• Changes in the protein profiles of plasma membrane preparations from Beta vulgaris roots as affected by Fe deficiency　　　　　　　　　　　　　　　
  Gutierrez-Carbonell E; Takahashi D; Luethje S; Uemura M; Abadia J; Lopez-Millan A-F
  13th Human Proteome Organization World Congress, Oct. 2014, [International conference]
  English, Poster presentation
• 生体分子添加水溶液にみられる集光フェムト秒レーザー誘起氷結晶成長２－結晶形状と成長速度の添加分子依存性－　　　　　　　　　　　　　　　
  河野達也; 澤田晃佑; 飯野敬矩; 三木雄史; 高橋大輔; 河村幸男; 上村松生; 細川陽一郎
  Sep. 2014, [Domestic conference]
  Japanese, Oral presentation
• 温度刺激に対する細胞膜マイクロドメインの組成および機能の応答　　　　　　　　　　　　　　　
  上村松生; 高橋大輔; 南杏鶴; 河村幸男
  Sep. 2014, [Domestic conference]
  Japanese, Oral presentation
• Changes in the protein profiles of plasma membrane and detergent resistant microdomain preparations from Beta vulgaris roots as affected by Fe deficiency　　　　　　　　　　　　　　　
  Gutierrez-Carbonell E; Takahashi D; Luethje S; Gonzalez-Reyes JA; Uemura M; Abadia J; Lopez-Millan A-F
  1st INPPO World Congress 2014, Aug. 2014, [Invited], [International conference]
  English, Poster presentation
• Tissue specific physiological and proteomic analysis of cold acclimation in the cell walls of winter wheat Triticum aestivum L. crown tissue　　　　　　　　　　　　　　　
  Willick I R; Takahashi D; Uemura M; Fowler D B; Tanino KK
  10th International Plant Cold Hardiness Seminar, Aug. 2014, [International conference]
  English, Poster presentation
• A cold acclimation-responsive glycosylphosphatidylinositol-anchored protein (GPI-AP) influences the acquisition of freezing tolerance in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  The 10th International Plant Cold Hardiness Seminar, Aug. 2014, [International conference]
  English, Oral presentation
• A cold acclimation-responsive glycosylphosphatidylinositol-anchored protein (GPI-AP) influences the acquisition of freezing tolerance in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D
  Research Seminar in Max Planck Institute for Molecular Plant Physiology, Potsdam, Germany, Aug. 2014, [Invited]
  English, Public discourse, Max Planck Institute for Molecular Plant Physiology, Potsdam, Germany
• Proteomic approaches reveal diversified responses of GPI-anchored proteins to cold acclimation in Arabidopsis.　　　　　　　　　　　　　　　
  Takahashi D
  Research Seminar in Estacion Experimental del Zaidin CSIC, Granada, Spain, Jul. 2014, [Invited]
  English, Public discourse, Estación Experimental del Zaidín CSIC, Granada, Spain
• Plasma membrane proteome dynamically and strategically responds to cold acclimation in plants　　　　　　　　　　　　　　　
  Uemura M; Takahashi D; Nakayama T; Miki Y; Kawamura Y
  The 6th International Symposium on Frontiers in Agricultural Proteome Research, Jun. 2014, [Invited], [International conference]
  English, Invited oral presentation
• Proteomic approaches reveal diversified responses of GPI-anchored proteins to cold acclimation in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  The 6th International Symposium on Frontiers in Agriculture Proteome Research, Jun. 2014, [Invited], [International conference]
  English, Oral presentation
• Proteomic approaches reveal diversified responses of GPI-anchored proteins to cold acclimation in Arabidopsis　　　　　　　　　　　　　　　
  Takahashi D
  Research Seminar in Jilin University, Changchun, China, Jun. 2014, [Invited]
  English, Public discourse, Jilin University, Changchun, China
• Compositional and functional analyses of glycosylphosphatidylinositol-anchored protein (GPI-AP) during plant cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  CRYO2014, May 2014, [International conference]
  English, Oral presentation
• Compositional and functional analyses of glycosylphosphatidylinositol-anchored protein (GPI-AP) during plant cold acclimation　　　　　　　　　　　　　　　
  Takahashi D
  Research Seminar in North Carolina State University, Raleigh, NC, USA, May 2014, [Invited]
  English, Public discourse, North Carolina State University, Raleigh, NC, USA
• 急激な温度降下で生じるセントポーリア葉の傷害誘導メカニズム　　　　　　　　　　　　　　　
  大西美輪; 角浜憲明; 高橋大輔; 七條千津子; 石崎公庸; 深城英弘; 上村松生; 鈴木祥弘; 三村徹郎
  Mar. 2014, [Domestic conference]
  Japanese, Poster presentation
• 新規単子葉モデル植物・ミナトカモジグサにおける低温馴化機構　　　　　　　　　　　　　　　
  中山貴人; 高橋大輔; 河村幸男; 上村松生
  Mar. 2014, [Domestic conference]
  Japanese, Poster presentation
• 細胞膜プロトンポンプの活性化に関わるプロテインキナーゼの解析　　　　　　　　　　　　　　　
  南杏鶴; 中村英; 高橋大輔; 井上晋一郎; 高橋宏二; 上村松生; 木下俊則
  Mar. 2014, [Domestic conference]
  Japanese, Oral presentation
• An Arabidopsis thaliana GPI-anchored protein associated with cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Tominaga Y; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiologists, Mar. 2014, [Domestic conference]
  Japanese, Oral presentation
• 単子葉モデル植物ミナトカモジグサを用いた低温馴化機構の解析　　　　　　　　　　　　　　　
  中山貴人; 高橋大輔; 河村幸男; 上村松生
  Dec. 2013, [Domestic conference]
  Japanese, Poster presentation
• シロイヌナズナ低温馴化および脱馴化過程における細胞膜プロテオミクス:シグナル伝達関連タンパク質に着目して　　　　　　　　　　　　　　　
  三木雄史; 高橋大輔; 河村幸男; 上村松生
  Dec. 2013, [Domestic conference]
  Japanese, Oral presentation
• 水分屈性制御因子MIZ1と相互作用して水分屈性を調節する因子の探索　　　　　　　　　　　　　　　
  山崎 誠和; 小林 啓恵; 宮沢 豊; 藤井 伸治; 高橋 大輔; 河村 幸男; 上村 松生; 高橋 秀幸
  Dec. 2013, [Domestic conference]
  Japanese, Oral presentation
• GPI-anchored Proteinに着目した低温馴化応答機構の解明～GPI-Proteome解析を基盤として～　　　　　　　　　　　　　　　
  高橋大輔; 冨永陽子; 河村幸男; 上村松生
  Dec. 2013, [Domestic conference]
  Japanese, Oral presentation
• 低温馴化過程におけるGPIアンカー型タンパク質の解析　　　　　　　　　　　　　　　
  高橋大輔
  Oct. 2013, [Domestic conference]
  Japanese, Oral presentation
• 休眠移行期における樹木の冬芽の可溶性タンパク質組成の変動　　　　　　　　　　　　　　　
  鈴木伸吾; 高橋大輔; 桑原慎子; 上村松生; 荒川圭太
  Sep. 2013, [Domestic conference]
  Japanese, Poster presentation
• 低温馴化機構とGPIアンカータンパク質の関連性　　　　　　　　　　　　　　　
  高橋大輔; 冨永陽子; 河村幸男; 上村松生
  Sep. 2013, [Domestic conference]
  Japanese, Oral presentation
• Cold-acclimation-induced changes of plasma membrane proteome in Brachypodium distachyon　　　　　　　　　　　　　　　
  Nakayama T; Takahashi D; Kawamura Y; Uemura M
  International Wheat Genetics Symposium, Sep. 2013, [Invited], [International conference]
  English, Invited oral presentation
• Proteomic approaches revealed dynamic changes in Arabidopsis thaliana cellular processes during cold acclimation and deacclimation　　　　　　　　　　　　　　　
  Miki Y; Takahashi D; Kawamura Y; Uemura M
  25th Congress of the Scandinavian Plant Physiology Society, Aug. 2013, [International conference]
  English, Poster presentation
• Proteomic studies of GPI-anchored proteins and its response to cold acclimation in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Takahashi D; Kawamura Y; Uemura M
  The Scandinavian Plant Physiology Society, Aug. 2013, [International conference]
  English, Invited oral presentation
• Global proteomic analysis reveals cold acclimation-responsiveness of GPI-anchored proteins　　　　　　　　　　　　　　　
  Takahashi D
  The 1st Iwate University Plant Science Retreat, Aug. 2013, [Domestic conference]
  English, Keynote oral presentation
• 樹木の冬芽の自発休眠の解除期における可溶性タンパク質組成変化の分析　　　　　　　　　　　　　　　
  鈴木慎吾; 高橋大輔; 桑原慎子; 上村松生; 荒川圭太
  Jun. 2013, [Domestic conference]
  Japanese, Oral presentation
• シロイヌナズナの低温馴化および脱馴化機構に関する時間分解的細胞膜プロテオーム解析　　　　　　　　　　　　　　　
  三木雄史; 高橋大輔; 河村幸男; 上村松生
  Jun. 2013, [Domestic conference]
  Japanese, Oral presentation
• 春季にセイヨウハコヤナギの枝で起こるタンパク質変動のショットガンプロテオーム解析　　　　　　　　　　　　　　　
  春日純; 高橋大輔; 織部雄一郎; 河村幸男; 上村松生
  Mar. 2013, [Domestic conference]
  Japanese, Oral presentation
• Proteomic profiles of Pisum sativum inner and outer chloroplast envelope membranes　　　　　　　　　　　　　　　
  Gutierrez-Carbonell E; Takahashi D; Lattanzio G; Rodriguez-Celma J; Duy D; Philippar K; Kehr J; Uemura M; Abadia J; Lopez-Millan A-F
  International Workshop on Plant Membrane Biology XVI, Mar. 2013, [International conference]
  English, Poster presentation
• Global proteomic analysis reveals cold acclimation-responsiveness of GPI-anchored proteins in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Takahashi D; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiolosists, Mar. 2013, [Domestic conference]
  English, Oral presentation
• Proteomic profiles of Pisum sativum inner and outer chloroplast envelope membranes　　　　　　　　　　　　　　　
  Gutierrez-Carbonell E; Takahashi D; Lattanzio G; Philippar K; Uemura M; Abadia J; Lopez-Millan A-F
  5th Congress of the Spanish Proteomics Society, Feb. 2013, [Domestic conference]
  Spanish, Castilian, Poster presentation
• シロイヌナズナ低温馴化過程における細胞膜ダイナミン関連タンパク質の機能　　　　　　　　　　　　　　　
  近藤万里子; 冨永陽子; 高橋大輔; 南杏鶴; 河村幸男; 上村松生
  Dec. 2012, [Domestic conference]
  Japanese, Oral presentation
• シロイヌナズナ低温馴化および脱馴化過程における細胞膜プロテオーム解析　　　　　　　　　　　　　　　
  三木雄史; 高橋大輔; 河村幸男; 上村松生
  Dec. 2012, [Domestic conference]
  Japanese, Poster presentation
• 樹木冬芽の越冬過程における可溶性タンパク質の組成変化　　　　　　　　　　　　　　　
  鈴木伸吾; 高橋大輔; 遠藤圭太; 岡田香織; 上村松生; 荒川圭太
  Nov. 2012, [Domestic conference]
  Japanese, Poster presentation
• Comprehensive analysis of glycosylphosphatidylinositol-anchored proteins and lipids of the plasma membrane to understand their functional involvement in plant freezing tolerance　　　　　　　　　　　　　　　
  Takahashi D; Kawamura Y; Uemura M
  Lipid-Protein Interactions in Membranes: Implications for Health and Disease, Nov. 2012, [International conference]
  English, Poster presentation
• GPIアンカー型タンパク質の低温馴化応答性　　　　　　　　　　　　　　　
  高橋大輔
  Oct. 2012, [Domestic conference]
  Japanese, Oral presentation
• Comparable plasma membrane proteomics of Brachypodium distachyon with two cereals (oat and rye) in association with cold acclimation　　　　　　　　　　　　　　　
  Nakayama T; Takahashi D; Kawamura Y; Rahman A; Uemura M
  American Society of Plant Biologists Annual Meeting, Jul. 2012, [International conference]
  English, Poster presentation
• Proteomic approaches for analyzing responsiveness of glycosylphosphatidylinositol-anchored proteins to cold acclimation in oat and rye　　　　　　　　　　　　　　　
  Takahashi D; Yamashita T; Kawamura Y; Uemura M
  American Society of Plant Biologists Annual Meeting, Jul. 2012, [International conference]
  English, Poster presentation
• Semiquantitative plasma membrane proteomics of Brachypodium distachyon in association with cold acclimation　　　　　　　　　　　　　　　
  Nakayama T; Takahashi D; Kawamura Y; Rahman A; Uemura M
  Plant and Microbe Adaptations to Cold 2012, Jun. 2012, [International conference]
  English, Poster presentation
• Proteomic analysis of seasonal cold-deacclimation process in poplar phloem and xylem tissues　　　　　　　　　　　　　　　
  Kasuga J; Takahashi D; Kawamura Y; Uemura M
  Plant and Microbe Adaptations to Cold 2012, Jun. 2012, [International conference]
  English, Oral presentation
• Involvement of plasma membrane microdomains in cold acclimation and freezing tolerance in plants　　　　　　　　　　　　　　　
  Takahashi D; Kawamura Y; Uemura M
  Plant and Microbe Adaptations to the Cold 2012, Jun. 2012, [International conference]
  English, Poster presentation
• 低温馴化過程におけるB. distachyon細胞膜タンパク質の組成変動の解析　　　　　　　　　　　　　　　
  中山貴人; 高橋大輔; 河村幸男; Rahman Abidur; 上村松生
  May 2012, [Domestic conference]
  Japanese, Oral presentation
• Plasma membrane proteome analysis of Arabidopsis suspension cultured cells (T87 line) associated with freezing tolerance and growth phase　　　　　　　　　　　　　　　
  Li B; Takahashi D; Kawamura Y; Uemura M
  The 1st International Symposium on Plant Environmental Sensing, Mar. 2012, [International conference]
  English, Poster presentation
• シロイヌナズナダイナミン関連タンパク質の低温馴化過程における影響と葉序との相関　　　　　　　　　　　　　　　
  近藤万里子; 高橋大輔; 南杏鶴; 冨永陽子; 河村幸男; 上村松生
  Mar. 2012, [Domestic conference]
  Japanese, Poster presentation
• 新規イネ科モデル植物における凍結耐性試験及び細胞膜プロテオーム解析　　　　　　　　　　　　　　　
  中山貴人; 高橋大輔; 河村幸男; Abidur Rahman; 上村松生
  Mar. 2012, [Domestic conference]
  Japanese, Poster presentation
• Changes in lipid composition of plasma membrane microdomain and freezing tolerance in oat and rye during cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Imai H; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiologists, Mar. 2012, [Domestic conference]
  Japanese, Poster presentation
• Comparable plasma membrane proteomics of the novel model grass species Brachypodium distachyon with two cereals (oat and rye)　　　　　　　　　　　　　　　
  Nakayama T; Takahashi D; Kawamura Y; Uemura M
  3rd International Symposium on Frontiers in Agriculture Proteome Research, Nov. 2011, [International conference]
  English, Poster presentation
• Exploring of plasma membrane proteomes in monocotyledonous plants: from a model plant to real crops　　　　　　　　　　　　　　　
  Uemura M; Takahashi D; Nakayama T; Kawamura Y
  3rd International Symposium on Frontiers in Agriculture Proteome Research, Nov. 2011, [Invited], [International conference]
  English, Invited oral presentation
• Changes in plasma membrane proteome of Arabidopsis suspension cultured cells (T87 line) in relation to freezing tolerance　　　　　　　　　　　　　　　
  Li B; Takahashi D; Kawamura Y; Uemura M
  3rd International Symposium on Frontiers in Agriculture Proteome Research, Nov. 2011, [International conference]
  English, Oral presentation
• 脂質組成解析と定量的プロテオーム解析から見る植物細胞膜マイクロドメインの動態と凍結耐性との関係　　　　　　　　　　　　　　　
  高橋大輔
  Oct. 2011, [Domestic conference]
  Japanese, Oral presentation
• What can we learn about cold acclimation process from plasma membrane proteomics with various plant species?　　　　　　　　　　　　　　　
  Uemura M; Takahashi D; Li B; Kondo M; Nakayama T; Minami A; Kawamura Y
  9th International Plant Cold Hardiness Seminar, Jul. 2011, [Invited], [International conference]
  English, Invited oral presentation
• Proteomic approaches for analyzing and elucidating the role of microdomain during cold acclimation in oat and rye.　　　　　　　　　　　　　　　
  Takahashi D; Yamashita T; Kawamura Y; Uemura M
  The 9th International Plant Cold Hardiness Seminar, Jul. 2011, [International conference]
  English, Oral presentation
• 植物細胞膜マイクロドメインのタンパク質組成と低温応答性　　　　　　　　　　　　　　　
  高橋大輔
  Dec. 2010, [Domestic conference]
  Japanese, Oral presentation
• Relationship between protein compositions of detergent-resistant plasma membrane microdomains in oat and rye and its association with their freezing tolerance　　　　　　　　　　　　　　　
  Takahashi D; Yamashita T; Kawamura Y; Uemura M
  2nd International Symposium on Frontiers in Agriculture Proteome Research, Nov. 2010, [International conference]
  English, Poster presentation
• Adaptation strategy of temperate herbaceous plants for cold environments: plasma membrane compositions and functions　　　　　　　　　　　　　　　
  Uemura M; Takahashi D; Kondo M; Furuto A; Kawamura Y
  International Symposium on Biodiversity Science 2010, Aug. 2010, [International conference]
  English, Invited oral presentation
• Alterations of plasma membrane microdomains in oat and rye during cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Furuto A; Minami A; Kawamura Y; Uemura M
  CRYO2010, Jul. 2010, [International conference]
  English, Poster presentation
• Relationship between plasma membrane microdomain compositions and freezing tolerance in oat and rye.　　　　　　　　　　　　　　　
  Takahashi D; Furuto A; Minami A; Kamo M; Kawamura Y; Uemura M
  55th Japanese Society for Cryobiology and Cryothechnology, Jun. 2010, [Domestic conference]
  Japanese, Oral presentation
• Alterations in the lipid composition of plasma membrane microdomain in oat and rye during cold acclimation　　　　　　　　　　　　　　　
  Takahashi D; Furuto A; Minami A; Kawamura Y; Uemura M
  The Japanese Society of Plant Physiologists, Mar. 2010, [Domestic conference]
  Japanese, Poster presentation

• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS
• THE BOTANICAL SOCIETY OF JAPAN
• JAPANESE SOCIETY FOR CRYOBIOLOGY AND CRYOTECHNOLOGY

• 13ｔｈ International Plant Cold Hardiness Seminar, Student course lecturer　　　　　　　　　　　　　　　
  Panel chair etc
  Daisuke Takahashi, 22 Aug. 2024 - 22 Aug. 2024
  Academic society etc
• 第68回 低温生物工学会　　　　　　　　　　　　　　　
  Planning etc
  17 Jun. 2023 - 18 Jun. 2023
  Academic society etc
• 12th International Plant Cold Hardiness Seminar　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  Daisuke Takahashi, 08 Dec. 2021 - 14 Dec. 2021
  Academic society etc