Performance information

• Atypical endo-β-1,4-mannannases are necessary for normal glucomannan synthesis in Arabidopsis　　　　　　　　　　　　　　　
  Aina Kikuchi; Eriko Sato; Yoshihisa Yoshimi; Hironori Takasaki; Naho Nishigaki; Kimie Atsuzawa; Yasuko Kaneko; Masatoshi Yamaguchi; Daisuke Takahashi; Paul Dupree; Toshihisa Kotake
  Feb. 2025
  DOI：https://doi.org/10.1101/2025.02.21.639209
  DOI ID：10.1101/2025.02.21.639209, ORCID：179119572
• Defective pollen meiosis in Arabidopsis due to combined arabinan and galactan insufficiency　　　　　　　　　　　　　　　
  Takuma Kikuchi; Kouichi Soga; Toshihisa Kotake; Daisuke Takahashi
  Feb. 2025, [Last, Corresponding]
  DOI：https://doi.org/10.1101/2025.02.03.636199
  DOI ID：10.1101/2025.02.03.636199, ORCID：177630155
• Soluble sugars make a greater contribution than cell wall components to the variability of freezing tolerance in wheat cultivars
  Sushan Chowhan; Takuma Kikuchi; Momoka Ohashi; Tatsuya Kutsuno; Hiroto Handa; Toshihisa Kotake; Daisuke Takahashi
  Plant Biotechnology, Volume：41, Number：4, First page：401, Last page：415, Dec. 2024, [Reviewed], [Last, Corresponding]
  Japanese Society for Plant Cell and Molecular Biology, English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.24.0801a
  DOI ID：10.5511/plantbiotechnology.24.0801a, ISSN：1342-4580, eISSN：1347-6114
• Cytosolic UDP‐L‐arabinose synthesis by bifunctional UDP‐glucose 4‐epimerases in Arabidopsis
  Akira Umezawa; Mayuko Matsumoto; Hiroto Handa; Konatsu Nakazawa; Megumi Miyagawa; Georg J. Seifert; Daisuke Takahashi; Shinya Fushinobu; Toshihisa Kotake
  The Plant Journal, Apr. 2024, [Reviewed]
  SUMMARY
  
  L‐Arabinose (L‐Ara) is a plant‐specific sugar found in cell wall polysaccharides, proteoglycans, glycoproteins, and small glycoconjugates, which play physiologically important roles in cell proliferation and other essential cellular processes. L‐Ara is synthesized as UDP‐L‐arabinose (UDP‐L‐Ara) from UDP‐xylose (UDP‐Xyl) by UDP‐Xyl 4‐epimerases (UXEs), a type of de novo synthesis of L‐Ara unique to plants. In Arabidopsis, the Golgi‐localized UXE AtMUR4 is the main contributor to UDP‐L‐Ara synthesis. However, cytosolic bifunctional UDP‐glucose 4‐epimerases (UGEs) with UXE activity, AtUGE1, and AtUGE3 also catalyze this reaction. For the present study, we first examined the physiological importance of bifunctional UGEs in Arabidopsis. The uge1 and uge3 mutants enhanced the dwarf phenotype of mur4 and further reduced the L‐Ara content in cell walls, suggesting that bifunctional UGEs contribute to UDP‐L‐Ara synthesis. Through the introduction of point mutations exchanging corresponding amino acid residues between AtUGE1 with high UXE activity and AtUGE2 with low UXE activity, two mutations that increase relative UXE activity of AtUGE2 were identified. The crystal structures of AtUGE2 in complex forms with NAD⁺ and NAD⁺/UDP revealed that the UDP‐binding domain of AtUGE2 has a more closed conformation and smaller sugar‐binding site than bacterial and mammalian UGEs, suggesting that plant UGEs have the appropriate size and shape for binding UDP‐Xyl and UDP‐L‐Ara to exhibit UXE activity. The presented results suggest that the capacity for cytosolic synthesis of UDP‐L‐Ara was acquired by the small sugar‐binding site and several mutations of UGEs, enabling diversified utilization of L‐Ara in seed plants.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.16779
  DOI ID：10.1111/tpj.16779, ISSN：0960-7412, eISSN：1365-313X
• Structural changes in cell wall pectic polymers contribute to freezing tolerance induced by cold acclimation in plants　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kouichi Soga; Takuma Kikuchi; Tatsuya Kutsuno; Pengfei Hao; Kazuma Sasaki; Yui Nishiyama; Satoshi Kidokoro; Arun Sampathkumar; Antony Bacic; Kim L. Johnson; Toshihisa Kotake
  Current Biology, Mar. 2024, [Reviewed], [Lead, Corresponding]
  Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2024.01.045
  DOI ID：10.1016/j.cub.2024.01.045, ORCID：152710373
• On the Separate Metabolisms of UDP-Sugars and GDP-Sugars in Plants
  Toshihisa Kotake; Daisuke Takahashi
  Trends in Glycoscience and Glycotechnology, Volume：36, Number：209, First page：J6, Last page：J11, Jan. 2024, [Reviewed]
  Forum: Carbohydrates Coming of Age, Scientific journal
  DOI：https://doi.org/10.4052/tigg.2303.1j
  DOI ID：10.4052/tigg.2303.1j, ISSN：0915-7352, eISSN：1883-2113
• Plant type II arabinogalactan: Structural features and modification to increase functionality　　　　　　　　　　　　　　　
  Kanika Ghosh; Daisuke Takahashi; Toshihisa Kotake
  Carbohydrate Research, Volume：529, First page：108828, Last page：108828, Jul. 2023, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.carres.2023.108828
  DOI ID：10.1016/j.carres.2023.108828, ISSN：0008-6215
• Spatiotemporal Control of Ice Crystallization in Supercooled Water via an Ultrashort Laser Impulse.　　　　　　　　　　　　　　　
  Hozumi Takahashi; Tatsuya Kono; Kosuke Sawada; Satoru Kumano; Yuka Tsuri; Mihoko Maruyama; Masashi Yoshimura; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura; Seiichiro Nakabayashi; Yusuke Mori; Yoichiroh Hosokawa; Hiroshi Y Yoshikawa
  The journal of physical chemistry letters, First page：4394, Last page：4402, May 2023, [Reviewed], ［International magazine］
  Focused irradiation with ultrashort laser pulses realized the fine spatiotemporal control of ice crystallization in supercooled water. An effective multiphoton excitation at the laser focus generated shockwaves and bubbles, which acted as an impulse for inducing ice crystal nucleation. The impulse that was localized close to the laser focus and accompanied by a small temperature elevation allowed the precise position control of ice crystallization and its observation with spatiotemporal resolution of micrometers and microseconds using a microscope. To verify the versatility of this laser method, we also applied it using various aqueous systems (e.g., plant extracts). The systematic study of crystallization probability revealed that laser-induced cavitation bubbles play a crucial role in inducing ice crystal nucleation. This method can be used as a tool for studying ice crystallization dynamics in various natural and biological phenomena.
  English, Scientific journal
  DOI：https://doi.org/10.1021/acs.jpclett.3c00414
  DOI ID：10.1021/acs.jpclett.3c00414, PubMed ID：37154425
• LSPpred Suite: Tools for Leaderless Secretory Protein Prediction in Plants
  Andrew Lonsdale; Laura Ceballos-Laita; Daisuke Takahashi; Matsuo Uemura; Javier Abadía; Melissa J. Davis; Antony Bacic; Monika S. Doblin
  Plants, Mar. 2023, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.3390/plants12071428
  DOI ID：10.3390/plants12071428, ORCID：131417758
• Cross‐disciplinary insights into the mechanisms of plant cold hardiness: From molecules to ecosystems　　　　　　　　　　　　　　　
  Guillaume Charrier; Ian R. Willick; Daisuke Takahashi
  Physiologia Plantarum, Volume：175, First page：e13901, Mar. 2023, [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13901
  DOI ID：10.1111/ppl.13901, ORCID：133007880
• Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light　　　　　　　　　　　　　　　
  Kazusato Oikawa; Shino Goto-Yamada; Yasuko Hayashi; Daisuke Takahashi; Yoshitaka Kimori; Michitaro Shibata; Kohki Yoshimoto; Atsushi Takemiya; Maki Kondo; Kazumi Hikino; Akira Kato; Keisuke Shimoda; Haruko Ueda; Matsuo Uemura; Keiji Numata; Yoshinori Ohsumi; Ikuko Hara-Nishimura; Shoji Mano; Kenji Yamada; Mikio Nishimura
  Nature Communications, Volume：13, Number：1, First page：7493, Last page：7493, Dec. 2022, [Reviewed], ［International magazine］
  Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present evidence that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-022-35138-z
  DOI ID：10.1038/s41467-022-35138-z, ORCID：124156494, PubMed ID：36470866
• Temporal cell wall changes during cold acclimation and deacclimation and their potential involvement in freezing tolerance and growth
  Tatsuya Kutsuno; Sushan Chowhan; Toshihisa Kotake; Daisuke Takahashi
  Physiologia Plantarum, Dec. 2022, [Reviewed], [Last, Corresponding]
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13837
  DOI ID：10.1111/ppl.13837, ISSN：0031-9317, eISSN：1399-3054, ORCID：124048822
• In vivo structural modification of type II arabinogalactans with fungal endo-β-1, 6-galactanase in Arabidopsis　　　　　　　　　　　　　　　
  Aina Kikuchi; Katsuya Hara; Yoshihisa Yoshimi; Kouichi Soga; Toshihisa Kotake
  Frontiers in Plant Science, Volume：13, Nov. 2022, [Reviewed]
  Arabinogalactan-proteins (AGPs) are mysterious extracellular glycoproteins in plants. Although AGPs are highly conserved, their molecular functions remain obscure. The physiological importance of AGPs has been extensively demonstrated with β-Yariv reagent, which specifically binds to AGPs and upon introduction into cells, causes various deleterious effects including growth inhibition and programmed cell death. However, structural features of AGPs that determine their functions have not been identified with β-Yariv reagent. It is known that AGPs are decorated with large type II arabinogalactans (AGs), which are necessary for their functions. Type II AGs consist of a β-1,3-galactan main chain and β-1,6-galactan side chains with auxiliary sugar residues such as L-arabinose and 4-O-methyl-glucuronic acid. While most side chains are short, long side chains such as β-1,6-galactohexaose (β-1,6-Gal6) also exist in type II AGs. To gain insight into the structures important for AGP functions, in vivo structural modification of β-1,6-galactan side chains was performed in Arabidopsis. We generated transgenic Arabidopsis plants expressing a fungal endo-β-1,6-galactanase, Tv6GAL, that degrades long side chains specifically under the control of dexamethasone (Dex). Two of 6 transgenic lines obtained showed more than 40 times activity of endo-β-1,6-galactanase when treated with Dex. Structural analysis indicated that long side chains such as β-1,6-Gal5 and β-1,6-Gal6 were significantly reduced compared to wild-type plants. Tv6GAL induction caused retarded growth of seedlings, which had a reduced amount of cellulose in cell walls. These results suggest that long β-1,6-galactan side chains are necessary for normal cellulose synthesis and/or deposition as their defect affects cell growth in plants.
  Frontiers Media {SA}, Scientific journal
  DOI：https://doi.org/10.3389/fpls.2022.1010492
  DOI ID：10.3389/fpls.2022.1010492, ISSN：1664-462X, ORCID：123074886
• Plasma membrane proteomic changes of Arabidopsis DRP1E during cold acclimation in association with the enhancement of freezing tolerance　　　　　　　　　　　　　　　
  Etsuko Watanabe; Mariko Kondo; Md Mostafa Kamal; Matsuo Uemura; Daisuke Takahashi; Yukio Kawamura
  Physiologia Plantarum, First page：e13820, Nov. 2022, [Reviewed], [Last, Corresponding], ［International magazine］
  The freezing tolerance of plants that live in cold regions increases after exposure to low temperature, a process termed cold acclimation (CA). During CA, restructuring of the plasma membrane (PM) is important to enhance freezing tolerance. We have previously shown that the function of DYNAMIN-RELATED PROTEIN 1E (DRP1E), which regulates endocytosis by pinching vesicles from the PM, is associated with the enhancement of freezing tolerance during CA in Arabidopsis. DRP1E is predicted to play a role in reconstituting the PM composition during CA. In this study, to test the validity of this hypothesis, we studied the changes in PM proteome patterns induced by drp1e mutation. In a detailed physiological analysis, after 3 days of CA, only young leaves showed significantly less increase in freezing tolerance in the mutant than in the wild type (WT). Using nano-liquid chromatography-tandem mass spectrometry, 496 PM proteins were identified. Among these proteins, 81 or 71 proteins were specifically altered in the WT or the mutant, respectively, in response to CA. Principal component analysis showed that the proteomic pattern differed between the WT and the mutant upon cold acclimation, suggesting that DRP1E contributes to reconstruction of the PM during CA. Cluster analysis revealed that proteins that were significantly increased in the mutant after CA were biased toward glycosylphosphatidylinositol-anchored proteins, such as fasciclin-like arabinogalactan proteins. Thus, a primary target of DRP1E-associated PM reconstruction during CA is considered to be glycosylphosphatidylinositol-anchored proteins, which may be removed from the PM by DRP1E in young leaves after 3 days of CA.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13820
  DOI ID：10.1111/ppl.13820, ORCID：122224080, PubMed ID：36335535
• Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (Solanum lycopersicum) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses
  Laura Ceballos-Laita; Daisuke Takahashi; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán; Jorge Rodríguez-Celma
  International Journal of Molecular Sciences, Volume：23, Number：7, First page：3719, Last page：3719, Mar. 2022, [Reviewed]
  Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
  {MDPI} {AG}, English, Scientific journal
  DOI：https://doi.org/10.3390/ijms23073719
  DOI ID：10.3390/ijms23073719, eISSN：1422-0067, ORCID：110644125
• Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.　　　　　　　　　　　　　　　
  Naho Nishigaki; Yoshihisa Yoshimi; Hiroaki Kuki; Tadashi Kunieda; Ikuko Hara-Nishimura; Yoichi Tsumuraya; Daisuke Takahashi; Paul Dupree; Toshihisa Kotake
  Physiologia plantarum, Volume：173, Number：3, First page：1244, Last page：1252, Aug. 2021, [Reviewed], ［International magazine］
  Cell wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases. However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear. GDP-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as substrate in the synthesis of mannan polysaccharides. GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis. Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan. Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan. On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure. These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars. This article is protected by copyright. All rights reserved.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13519
  DOI ID：10.1111/ppl.13519, PubMed ID：34380178
• Responses of the Plant Cell Wall to Sub-Zero Temperatures: A Brief Update
  Daisuke Takahashi; Ian R Willick; Jun Kasuga; David P Livingston III
  Plant and Cell Physiology, Jul. 2021, [Reviewed], [Lead, Corresponding]
  
  Our general understanding of plant responses to sub-zero temperatures focuses on mechanisms that mitigate stress to the plasma membrane. The plant cell wall receives comparatively less attention and questions surrounding its role in mitigating freezing injury remains unresolved. Despite recent molecular discoveries that provide insight into acclimation responses, the goal of reducing freezing injury in herbaceous and woody crops remains elusive. This is likely due to the complexity associated with adaptations to low temperatures. Understanding how leaf cell walls of herbaceous annuals promote tissue tolerance to ice does not necessarily lead to understanding how meristematic tissues are protected from freezing by tissue-level barriers formed by cell walls in overwintering tree buds. In this mini-review, we provide an overview of biological ice nucleation and explain how plants control the spatiotemporal location of ice formation. We discuss how sugars and pectin side chains alleviate adhesive injury that develops at sub-zero temperatures between the matrix polysaccharides and ice. The importance of site-specific cell wall elasticity to promote tissue expansion for ice accommodation and control of porosity to impede ice growth and promote supercooling will be presented. How specific cold-induced proteins modify plant cell walls to mitigate freezing injury will also be discussed. The opinions presented in this report emphasize the importance of a plant’s developmental physiology when characterizing mechanisms of freezing survival.
  Oxford University Press ({OUP}), English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab103
  DOI ID：10.1093/pcp/pcab103, ISSN：0032-0781, eISSN：1471-9053, ORCID：96808227
• Cold Acclimation Responses of Fasciclin-like Arabinogalactan Protein Genes in A. Thaliana　　　　　　　　　　　　　　　
  TAKAHASHI Daisuke; SHIBASAKI Yukino; KOTAKE Toshihisa
  Cryobiology and Cryotechnology, Volume：67, Number：1, First page：53, Last page：57, 2021, [Reviewed]
  Cold acclimation (CA) results in alterations of plasma membrane (PM) and cell wall proteins in plants,
  which is considered to be crucial for increasing their freezing tolerance. Fasciclin-like arabinogalactan
  proteins (FLAs) are abundant glycoproteins in the PM and cell wall and have potential roles in abiotic stress
  responses via transducing signaling and modulating cell wall functions. In present study, we investigated
  temporal expression of 21 FLA genes during CA in Arabidopsis thaliana. We found that there are a variety of
  changing patterns among FLAs in response to CA. Principal component analysis clearly showed that
  expression of FLAs and its responses to CA varied considerably between shoot and root. FLAs2, 8 and 13,
  the most abundant FLA genes, exhibited increases of their expression in the early stage of CA, but its extent
  was greatly different between shoot and root. Taken together, we will discuss about the potential role of
  FLAs in response to low temperature in plants.
  Japanese Society of Cryobiology and Cryotechnology, Japanese
  DOI：https://doi.org/10.20585/cryobolcryotechnol.67.1_53
  DOI ID：10.20585/cryobolcryotechnol.67.1_53, ISSN：1340-7902, eISSN：2424-1555, CiNii Articles ID：130008054042, CiNii Books ID：AN10448734
• Structural features conserved in subclass of type II arabinogalactan
  Kengo Ito; Kurumi Fukuoka; Naho Nishigaki; Katsuya Hara; Yoshihisa Yoshimi; Hiroaki Kuki; Daisuke Takahashi; Yoichi Tsumuraya; Toshihisa Kotake
  Plant Biotechnology, Volume：37, Number：4, First page：459, Last page：463, Dec. 2020, [Reviewed]
  Japanese Society for Plant Cell and Molecular Biology, English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.20.0721a
  DOI ID：10.5511/plantbiotechnology.20.0721a, ISSN：1347-6114, eISSN：1347-6114, ORCID：85846532
• Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis.　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Andrew Lonsdale; Anunciación Abadía; Monika S Doblin; Antony Bacic; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán
  International journal of molecular sciences, Volume：21, Number：22, First page：8863, Last page：8863, Nov. 2020, [Reviewed], ［International magazine］
  Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant's response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 µM MnCl2 as control and excess Mn treatments, respectively. This approach yielded 668 proteins reliably identified and quantified. Excess Mn caused statistically significant (at p ≤ 0.05) and biologically relevant changes in relative abundance (≥2-fold increases or ≥50% decreases) in 322 proteins, with 82% of them predicted to be secretory using three different prediction tools, with more decreasing than increasing (181 and 82, respectively), suggesting that this metal stress causes an overall deactivation of metabolic pathways. Processes most affected by excess Mn were in the oxido-reductase, polysaccharide and protein metabolism classes. Excess Mn induced changes in hydrolases and peroxidases involved in cell wall degradation and lignin formation, respectively, consistent with the existence of alterations in the cell wall. Protein turnover was also affected, as indicated by the decrease in proteolytic enzymes and protein synthesis-related proteins. Excess Mn modified the redox environment of the xylem sap, with changes in the abundance of oxido-reductase and defense protein classes indicating a stress scenario. Finally, results indicate that excess Mn decreased the amounts of proteins associated with several signaling pathways, including fasciclin-like arabinogalactan-proteins and lipids, as well as proteases, which may be involved in the release of signaling peptides and protein maturation. The comparison of the proteins changing in abundance in xylem sap and roots indicate the existence of tissue-specific and systemic responses to excess Mn. Data are available via ProteomeXchange with identifier PXD021973.
  {MDPI} {AG}, English, Scientific journal
  DOI：https://doi.org/10.3390/ijms21228863
  DOI ID：10.3390/ijms21228863, ISSN：1422-0067, ORCID：84202246, PubMed ID：33238539, PubMed Central ID：PMC7700171
• Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub‐zero acclimation　　　　　　　　　　　　　　　
  Daisuke Takahashi; Kim Johnson; Pengfei Hao; Tan Tuong; Alex; er Erban; Arun Sampathkumar; Antony Bacic; David P. Livingston; Joachim Kopka; Takeshi Kuroha; Ryusuke Yokoyama; Kazuhiko Nishitani; Ellen Zuther; Dirk K. Hincha
  Plant, Cell & Environment, Volume：44, Number：3, First page：915, Last page：930, Nov. 2020, [Reviewed], [Lead, Corresponding], ［International magazine］
  Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle. Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation. In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation). Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation. In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH). The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure. Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant. Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/pce.13953
  DOI ID：10.1111/pce.13953, ISSN：1365-3040, ORCID：83520467, PubMed ID：33190295
• Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS.　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  Methods in molecular biology (Clifton, N.J.), Volume：2139, First page：89, Last page：106, 2020, [Reviewed], [Lead], ［International magazine］
  Shotgun proteomics allows for the comprehensive analysis of proteins extracted from plant cells, subcellular organelles, and membranes. Previously, two-dimensional gel electrophoresis-based proteomics was used for mass spectrometric analysis of plasma membrane proteins. However, this method is not fully applicable for highly hydrophobic proteins with multiple transmembrane domains. In order to solve this problem, we here describe a shotgun proteomics method using nano-LC-MS/MS for proteins in the plasma membrane and plasma membrane microdomain fractions. The results obtained are easily applicable to label-free protein semiquantification.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0528-8_7
  DOI ID：10.1007/978-1-0716-0528-8_7, PubMed ID：32462580
• Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins.　　　　　　　　　　　　　　　
  Md Mostafa Kamal; Daisuke Takahashi; Takato Nakayama; Yushi Miki; Yukio Kawamura; Matsuo Uemura
  Methods in molecular biology (Clifton, N.J.), Volume：2156, First page：171, Last page：186, 2020, [Reviewed], ［International magazine］
  Plasma membrane is the primary determinant of freezing tolerance in plants because of its central role in freeze-thaw cycle. Changes in plasma membrane protein composition have been one of the major research areas in plant cold acclimation. To obtain comprehensive profiles of the plasma membrane proteomes and their changes during the cold acclimation process, a plasma membrane purification method using a dextran-polyethylene glycol two polymer system and a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for the plasma membrane proteins are described. The proteomic results obtained are further applied to label-free protein semiquantification.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0660-5_13
  DOI ID：10.1007/978-1-0716-0660-5_13, PubMed ID：32607982
• Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.　　　　　　　　　　　　　　　
  Daisuke Takahashi; Ellen Zuther; Dirk K Hincha
  Methods in molecular biology (Clifton, N.J.), Volume：2156, First page：255, Last page：268, 2020, [Reviewed], [Lead], ［International magazine］
  The cell wall has a crucial influence on the mechanical properties of plant cells. It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants. However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation. In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation. We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation. Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.
  English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-0716-0660-5_17
  DOI ID：10.1007/978-1-0716-0660-5_17, PubMed ID：32607986
• Plasma membrane proteome analyses of Arabidopsis thaliana suspension-cultured cells during cold or ABA treatment: Relationship with freezing tolerance and growth phase.　　　　　　　　　　　　　　　
  Li B; Takahashi D; Kawamura Y; Uemura M
  Journal of proteomics, Volume：211, First page：103528, Last page：103528, Sep. 2019, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2019.103528
  DOI ID：10.1016/j.jprot.2019.103528, ISSN：1874-3919, PubMed ID：31541795
• Temporal proteomics of Arabidopsis plasma membrane during cold- and de-acclimation　　　　　　　　　　　　　　　
  Yushi Miki; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Journal of Proteomics, Volume：197, First page：71, Last page：81, Apr. 2019, [Reviewed], [Lead]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2018.11.008
  DOI ID：10.1016/j.jprot.2018.11.008, ISSN：1874-3919, ORCID：78380633, PubMed ID：30447334
• Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Daisuke Takahashi; Michal Gorka; Alexander Erban; Alexander Graf; Joachim Kopka; Ellen Zuther; Dirk K. Hincha
  Scientific Reports, Volume：9, Number：1, Feb. 2019, [Reviewed], [Lead]
  Abstract
  
  Cold acclimation (CA) leads to increased plant freezing tolerance during exposure to low, non-freezing temperatures as a result of many physiological, biochemical and molecular changes that have been extensively investigated. In addition, many plant species, such asArabidopsis thaliana, respond to a subsequent exposure to mild, non-damaging freezing temperatures with an additional increase in freezing tolerance referred to as sub-zero acclimation (SZA). There is comparatively little information available about the molecular basis of SZA. However, previous transcriptomic studies indicated that cell wall modification may play an important role during SZA. Here we show that CA and SZA are accompanied by extensive changes in cell wall amount, composition and structure. While CA leads to a significant increase in cell wall amount, the relative proportions of pectin, hemicellulose and cellulose remained unaltered during both CA and SZA. However, both treatments resulted in more subtle changes in structure as determined by infrared spectroscopy and monosaccharide composition as determined by gas chromatography-mass spectrometry. These differences could be related through a proteomic approach to the accumulation of cell wall modifying enzymes such as pectin methylesterases, pectin methylesterase inhibitors and xyloglucan endotransglucosylases/hydrolases in the extracellular matrix.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s41598-019-38688-3
  DOI ID：10.1038/s41598-019-38688-3, ISSN：2045-2322, eISSN：2045-2322, ORCID：78380625, PubMed ID：30783145
• Data on xylem sap proteins from Mn- and Fe-deficient tomato plants obtained using shotgun proteomics　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Anunciación Abadía; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán
  Data in Brief, Volume：17, First page：512, Last page：516, Apr. 2018, [Reviewed]
  Elsevier Inc., English, Scientific journal
  DOI：https://doi.org/10.1016/j.dib.2018.01.034
  DOI ID：10.1016/j.dib.2018.01.034, ISSN：2352-3409, PubMed ID：29876421, SCOPUS ID：85041676938
• Tissue-specific changes in apoplastic proteins and cell wall structure during cold acclimation of winter wheat crowns　　　　　　　　　　　　　　　
  Ian R Willick; Daisuke Takahashi; D Brian Fowler; Matsuo Uemura; Karen K. Tanino
  Journal of Experimental Botany, Volume：69, Number：5, First page：1221, Last page：1234, Feb. 2018, [Reviewed]
  Oxford University Press, English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erx450
  DOI ID：10.1093/jxb/erx450, ISSN：1460-2431, PubMed ID：29373702, SCOPUS ID：85042625541
• Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses　　　　　　　　　　　　　　　
  Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Daisuke Takahashi; Anunciacion Abadia; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOMICS, Volume：170, First page：117, Last page：129, Jan. 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jprot.2017.08.018
  DOI ID：10.1016/j.jprot.2017.08.018, ISSN：1874-3919, eISSN：1876-7737, PubMed ID：28847647, Web of Science ID：WOS:000415778000011
• Plasma membrane proteomics of arabidopsis suspension-cultured cells associated with growth phase using Nano-LC-MS/MS　　　　　　　　　　　　　　　
  Bin Li; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1696, First page：185, Last page：194, 2018, [Reviewed]
  Humana Press Inc., English, In book
  DOI：https://doi.org/10.1007/978-1-4939-7411-5_12
  DOI ID：10.1007/978-1-4939-7411-5_12, ISSN：1064-3745, PubMed ID：29086404, SCOPUS ID：85032646702
• Freezing Tolerance of Plant Cells: From the Aspect of Plasma Membrane and Microdomain　　　　　　　　　　　　　　　
  Daisuke Takahashi
  Advances in Experimental Medicine and Biology, Volume：1081, First page：61, Last page：79, 2018, [Reviewed], [Lead]
  DOI：https://doi.org/10.1007/978-981-13-1244-1_4
  DOI ID：10.1007/978-981-13-1244-1_4, ISSN：0065-2598, ORCID：78380660, PubMed ID：30288704
• Isolation of plasma membrane and plasma membrane microdomains　　　　　　　　　　　　　　　
  Anzu Minami; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1511, First page：199, Last page：212, 2017, [Reviewed]
  Humana Press Inc., English, In book
  DOI：https://doi.org/10.1007/978-1-4939-6533-5_16
  DOI ID：10.1007/978-1-4939-6533-5_16, ISSN：1064-3745, PubMed ID：27730613, SCOPUS ID：84991457583
• Cold acclimation is accompanied by complex responses of glycosylphosphatidylinositol (GPI)-anchored proteins in Arabidopsis　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：67, Number：17, First page：5203, Last page：5215, Sep. 2016, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erw279
  DOI ID：10.1093/jxb/erw279, ISSN：0022-0957, eISSN：1460-2431, PubMed ID：27471282, Web of Science ID：WOS:000384648900022
• A Shotgun Proteomic Approach Reveals That Fe Deficiency Causes Marked Changes in the Protein Profiles of Plasma Membrane and Detergent-Resistant Microdomain Preparations from Beta vulgaris Roots　　　　　　　　　　　　　　　
  Elain Gutierrez-Carbonell; Daisuke Takahashi; Sabine Luethje; Jose Antonio Gonzalez-Reyes; Sebastien Mongrand; Bruno Contreras-Moreira; Anunciacion Abadia; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOME RESEARCH, Volume：15, Number：8, First page：2510, Last page：2524, Aug. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1021/acs.jproteome.6b00026
  DOI ID：10.1021/acs.jproteome.6b00026, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：27321140, Web of Science ID：WOS:000381235900015
• Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Hiroyuki Imai; Yukio Kawamura; Matsuo Uemura
  CRYOBIOLOGY, Volume：72, Number：2, First page：123, Last page：134, Apr. 2016, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cryobiol.2016.02.003
  DOI ID：10.1016/j.cryobiol.2016.02.003, ISSN：0011-2240, eISSN：1090-2392, PubMed ID：26904981, Web of Science ID：WOS:000374078600008
• Proteins Associated with Oxidative Burst and Cell Wall Strengthening Accumulate During Citrus-Xanthomonas Non-Host Interaction　　　　　　　　　　　　　　　
  T. Swaroopa Rani; Daisuke Takahashi; Matsuo Uemura; Appa Rao Podile
  PLANT MOLECULAR BIOLOGY REPORTER, Volume：33, Number：5, First page：1349, Last page：1360, Oct. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11105-014-0817-y
  DOI ID：10.1007/s11105-014-0817-y, ISSN：0735-9640, eISSN：1572-9818, SCOPUS ID：84914128960, Web of Science ID：WOS:000360392800014
• The Distinct Functional Roles of the Inner and Outer Chloroplast Envelope of Pea (Pisum sativum) As Revealed by Proteomic Approaches　　　　　　　　　　　　　　　
  Elain Gutierrez-Carbonell; Daisuke Takahashi; Giuseppe Lattanzio; Jorge Rodriguez-Celma; Julia Kehr; Juergen Soll; Katrin Philippar; Matsuo Uemura; Javier Abadia; Ana Flor Lopez-Millan
  JOURNAL OF PROTEOME RESEARCH, Volume：13, Number：6, First page：2941, Last page：2953, Jun. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1021/pr500106s
  DOI ID：10.1021/pr500106s, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：24792535, Web of Science ID：WOS:000337074500022
• Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins　　　　　　　　　　　　　　　
  Daisuke Takahashi
  Methods in Molecular Biology, Volume：1166, First page：159, Last page：170, May 2014, [Reviewed], [Lead]
  DOI：https://doi.org/10.1007/978-1-4939-0844-8_13
  DOI ID：10.1007/978-1-4939-0844-8_13, ISSN：1064-3745, ORCID：78380684, PubMed ID：24852635
• Temporal Analysis of Plasma Membrane Proteome in Arabidopsis during Cold Acclimation and Deacclimation　　　　　　　　　　　　　　　
  MIKI YUUSHI; TAKAHASHI DAISUKE; KAWAMURA YUKIO; UEMURA MATSUO
  低温生物工学会誌, Volume：60, Number：1, First page：35, Last page：41, Apr. 2014, [Reviewed]
  Overwintering temperate plants can enhance freezing tolerance in response to low, non-freezing temperatures (cold acclimation, CA). When cold-acclimated plants are transferred to a warm temperature, they reversibly lose their freezing tolerance rather quickly (deacclimation, DA). Particularly, DA is important under oscillatory temperature conditions in early spring in the field but has not been studied in detail. Thus, the purpose of this study is to accumulate fundamental knowledge of DA to explore possibilities to reduce freezing injury occurring in the DA period. First, we verified changes of freezing tolerance in Arabidopsis thaliana during CA and DA periods: acquired freezing tolerance after 1 week of CA at 2℃ was lost after 2 days of DA. Next, we focused on proteomic changes of the plasma membrane that is considered to be the primary site for determination of freezing tolerance. Proteome analysis identified 647 CA and/or DA responsive-proteins with different patterns of alternations during CA and DA periods and these proteins were categorized into specific functional categories. With these results, we discussed the function of CA and DA responsive-proteins in detail.
  Japanese Society for Cryobiology and Cryotechnology, Japanese
  ISSN：1340-7902, J-Global ID：201402271206507220, CiNii Articles ID：110009810731, CiNii Books ID：AN10448734
• Compositional Changes in Soluble Proteins of Tree Buds During Endodormancy Release　　　　　　　　　　　　　　　
  SUZUKI Shingo; TAKAHASHI Daisuke; KUWABARA Chikako; UEMURA Matsuo; ARAKAWA Keita
  Cryobiology and Cryotechnology, Volume：60, Number：1, First page：59, Last page：63, 2014, [Reviewed]
  In this study, we analyzed soluble proteins in winter bud during endodormancy release. Endodormancy of winter buds of Salix bakko and Larix kaempferi was released clearly in early and late December, respectively. SDS-PAGE analysis showed that soluble protein composition of buds in each species hardly changed in a short period of endodormancy release. Proteome analysis using LC-MS/MS revealed compositional changes in soluble proteins in this period of endodormancy release. However, the proportion of soluble proteins whose levels were changed in this period was under 5% of all detected proteins in each species of winter buds, and the variation was small.
  Japanese Society of Cryobiology and Cryotechnology, Japanese
  DOI：https://doi.org/10.20585/cryobolcryotechnol.60.1_59
  DOI ID：10.20585/cryobolcryotechnol.60.1_59, ISSN：1340-7902, J-Global ID：201402272670661654, CiNii Articles ID：110009810735, CiNii Books ID：AN10448734
• Shotgun proteomics of plant plasma membrane and microdomain proteins using Nano-LC-MS/MS　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  Methods in Molecular Biology, Volume：1072, First page：481, Last page：498, 2014, [Reviewed], [Lead]
  Humana Press Inc., English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-62703-631-3_33
  DOI ID：10.1007/978-1-62703-631-3_33, ISSN：1064-3745, PubMed ID：24136542, SCOPUS ID：84934439028
• Changes of Detergent-Resistant Plasma Membrane Proteins in Oat and Rye during Cold Acclimation: Association with Differential Freezing Tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  JOURNAL OF PROTEOME RESEARCH, Volume：12, Number：11, First page：4998, Last page：5011, Nov. 2013, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1021/pr400750g
  DOI ID：10.1021/pr400750g, ISSN：1535-3893, eISSN：1535-3907, PubMed ID：24111712, Web of Science ID：WOS:000326615000026
• Plant plasma membrane proteomics for improving cold tolerance　　　　　　　　　　　　　　　
  Daisuke Takahashi; Bin Li; Takato Nakayama; Yukio Kawamura; Matsuo Uemura
  FRONTIERS IN PLANT SCIENCE, Volume：4, First page：90, Apr. 2013, [Reviewed], [Lead]
  English
  DOI：https://doi.org/10.3389/fpls.2013.00090
  DOI ID：10.3389/fpls.2013.00090, ISSN：1664-462X, PubMed ID：23616787, Web of Science ID：WOS:000329825800001
• Compositional changes of plasma membrane proteins of Brachypodium distachyon during cold acclimation　　　　　　　　　　　　　　　
  NAKAYAMA TAKATO; TAKAHASHI DAISUKE; KAWAMURA YUKIO; RAHMAN ABIDUR; UEMURA MATSUO
  低温生物工学会誌, Volume：59, Number：1, First page：61, Last page：65, Apr. 2013, [Reviewed]
  To survive at freezing temperatures, temperate plants increase their freezing tolerance by cold acclimation, during which changes of protein and lipid compositions of the plasma membrane (PM) play a key role. Brachypodium distachyon, a temperate grass species, has been recognized as a model plant and is evolutionarily closer to temperate cereals than rice. These facts suggest that B. distachyon could serve as a good system to study freezing tolerance mechanism of temperate cereals. In the present study, using non-acclimated (NA) and cold-acclimated (CA, 2℃) B. distachyon, we performed freezing tolerance test and PM proteome analysis. When freezing tolerance was evaluated by measurement of electrolyte leakage, the temperature at which 50% electrolyte leakage occurs after freezing (T_) was lowered from -3.9℃ to -6.9℃ by cold acclimation. Furthermore based on the proteome analysis, PM proteins were found to be changed considerably by cold acclimation and the changes were similar in some parts to those found in oat and rye during cold acclimation. These results suggest that B. distachyon has an ability of cold acclimation and some common pathways responding to cold as shown in temperate cereals.
  Japanese Society for Cryobiology and Cryotechnology, Japanese
  ISSN：1340-7902, J-Global ID：201302245157841545, CiNii Articles ID：110009612769, CiNii Books ID：AN10448734
• Detergent-resistant plasma membrane proteome to elucidate microdomain functions in plant cells　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  Frontiers in Plant Science, Volume：4, First page：27, Feb. 2013, [Reviewed], [Lead]
  Frontiers Research Foundation, English
  DOI：https://doi.org/10.3389/fpls.2013.00027
  DOI ID：10.3389/fpls.2013.00027, ISSN：1664-462X, PubMed ID：23440896, SCOPUS ID：84900836694
• 樹木冬芽の越冬過程における可溶性タンパク質の組成変化　　　　　　　　　　　　　　　
  鈴木伸吾; 遠藤圭太; 岡田香織; 荒川圭太; 高橋大輔; 上村松生
  日本木材学会北海道支部講演集(Web), Number：44, First page：WEB ONLY P-2, Nov. 2012, [Reviewed]
  Japanese
  J-Global ID：201202266951323958
• Function of Arabidopsis dynamin-related protein 1E during cold acclimation　　　　　　　　　　　　　　　
  KONDO MARIKO; TAKAHASHI DAISUKE; MINAMI ANZU; KAWAMURA YUKIO; UEMURA MATSUO
  低温生物工学会誌, Volume：58, Number：1, First page：105, Last page：110, Apr. 2012, [Reviewed]
  Japanese
  ISSN：1340-7902, J-Global ID：201202212052041990
• Detergent-resistant Plasma Membrane Proteome in Oat and Rye: Similarities and Dissimilarities between Two Monocotyledonous Plants　　　　　　　　　　　　　　　
  Daisuke Takahashi; Yukio Kawamura; Tetsuro Yamashita; Matsuo Uemura
  JOURNAL OF PROTEOME RESEARCH, Volume：11, Number：3, First page：1654, Last page：1665, Mar. 2012, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1021/pr200849v
  DOI ID：10.1021/pr200849v, ISSN：1535-3893, PubMed ID：22191623, Web of Science ID：WOS:000300916200020
• Comparison of Plasma Membrane Proteomic Changes of Arabidopsis Suspension-Cultured Cells (T87 Line) after Cold and ABA Treatment in Association with Freezing Tolerance Development　　　　　　　　　　　　　　　
  Bin Li; Daisuke Takahashi; Yukio Kawamura; Matsuo Uemura
  PLANT AND CELL PHYSIOLOGY, Volume：53, Number：3, First page：543, Last page：554, Mar. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcs010
  DOI ID：10.1093/pcp/pcs010, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：22318864, Web of Science ID：WOS:000301359500005
• Relationship of Plasma Membrane Microdomain Composition and Freezing Tolerance in Oat and Rye　　　　　　　　　　　　　　　
  TAKAHASHI DAISUKE; FURUTO AKARI; MINAMI ANZU; KAMO MASAHARU; YAMASHITA TETSURO; UEMURA MATSUO
  低温生物工学会誌, Volume：57, Number：1, First page：95, Last page：99, Apr. 2011, [Reviewed], [Lead]
  Japanese
  ISSN：1340-7902, J-Global ID：201102251181376530

• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS
• THE BOTANICAL SOCIETY OF JAPAN
• JAPANESE SOCIETY FOR CRYOBIOLOGY AND CRYOTECHNOLOGY

• 13ｔｈ International Plant Cold Hardiness Seminar, Student course lecturer　　　　　　　　　　　　　　　
  Panel chair etc
  Daisuke Takahashi, 22 Aug. 2024 - 22 Aug. 2024
  Academic society etc
• 第68回 低温生物工学会　　　　　　　　　　　　　　　
  Planning etc
  17 Jun. 2023 - 18 Jun. 2023
  Academic society etc
• 12th International Plant Cold Hardiness Seminar　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  Daisuke Takahashi, 08 Dec. 2021 - 14 Dec. 2021
  Academic society etc