Performance information

• A tonoplast-localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high Mg2+ conditions.　　　　　　　　　　　　　　　
  Shin-Ichiro Inoue; Maki Hayashi; Sheng Huang; Kengo Yokosho; Eiji Gotoh; Shuka Ikematsu; Masaki Okumura; Takamasa Suzuki; Takumi Kamura; Toshinori Kinoshita; Jian Feng Ma
  The New phytologist, Number：236, First page：864, Last page：877, Jul. 2022, [Reviewed], [Lead, Corresponding], ［International magazine］
  Plant stomata play an important role in CO2 uptake for photosynthesis and transpiration, but the mechanisms underlying stomatal opening and closing under changing environmental conditions are still not completely understood. Through large-scale genetic screening, we isolated an Arabidopsis mutant (closed stomata2 (cst2)) that is defective in stomatal opening. We cloned the causal gene (MGR1/CST2) and functionally characterized this gene. The mutant phenotype was caused by a mutation in a gene encoding an unknown protein with similarities to the human magnesium (Mg2+ ) efflux transporter ACDP/CNNM. MGR1/CST2 was localized to the tonoplast and showed transport activity for Mg2+ . This protein was constitutively and highly expressed in guard cells. Knockout of this gene resulted in stomatal closing, decreased photosynthesis and growth retardation, especially under high Mg2+ conditions, while overexpression of this gene increased stomatal opening and tolerance to high Mg2+ concentrations. Furthermore, guard cell-specific expression of MGR1/CST2 in the mutant partially restored its stomatal opening. Our results indicate that MGR1/CST2 expression in the leaf guard cells plays an important role in maintaining cytosolic Mg2+ concentrations through sequestering Mg2+ into vacuoles, which is required for stomatal opening, especially under high Mg2+ conditions.
  English, Scientific journal
  DOI：https://doi.org/10.1111/nph.18410
  DOI ID：10.1111/nph.18410, PubMed ID：35976788
• Type 2C protein phosphatase clade D family members dephosphorylate guard cell plasma membrane H+-ATPase
  Mitsumasa Akiyama; Hodaka Sugimoto; Shin-ichiro Inoue; Yohei Takahashi; Maki Hayashi; Yuki Hayashi; Miya Mizutani; Takumi Ogawa; Daichi Kinoshita; Eigo Ando; Meeyeon Park; William M Gray; Toshinori Kinoshita
  Plant Physiology, Volume：188, Number：4, First page：2228, Last page：2240, Mar. 2022, [Reviewed]
  Abstract
  
  Plasma membrane (PM) H+-ATPase in guard cells is activated by phosphorylation of the penultimate residue, threonine (Thr), in response to blue and red light, promoting stomatal opening. Previous in vitro biochemical investigation suggested that Mg2+- and Mn2+-dependent membrane-localized type 2C protein phosphatase (PP2C)-like activity mediates the dephosphorylation of PM H+-ATPase in guard cells. PP2C clade D (PP2C.D) was later demonstrated to be involved in PM H+-ATPase dephosphorylation during auxin-induced cell expansion in Arabidopsis (Arabidopsis thaliana). However, it is unclear whether PP2C.D phosphatases are involved in PM H+-ATPase dephosphorylation in guard cells. Transient expression experiments using Arabidopsis mesophyll cell protoplasts revealed that all PP2C.D isoforms dephosphorylate the endogenous PM H+-ATPase. We further analyzed PP2C.D6/8/9, which display higher expression levels than other isoforms in guard cells, observing that pp2c.d6, pp2c.d8, and pp2c.d9 single mutants showed similar light-induced stomatal opening and phosphorylation status of PM H+-ATPase in guard cells as Col-0. In contrast, the pp2c.d6/9 double mutant displayed wider stomatal apertures and greater PM H+-ATPase phosphorylation in response to blue light, but delayed dephosphorylation of PM H+-ATPase in guard cells; the pp2c.d6/8/9 triple mutant showed similar phenotypes to those of the pp2c.d6/9 double mutant. Taken together, these results indicate that PP2C.D6 and PP2C.D9 redundantly mediate PM H+-ATPase dephosphorylation in guard cells. Curiously, unlike auxin-induced cell expansion in seedlings, auxin had no effect on the phosphorylation status of PM H+-ATPase in guard cells.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiab571
  DOI ID：10.1093/plphys/kiab571, ISSN：0032-0889, eISSN：1532-2548
• Phototropin 2 contributes to the chloroplast avoidance response at the chloroplast-plasma membrane interface.　　　　　　　　　　　　　　　
  Kazuhiro Ishishita; Takeshi Higa; Hidekazu Tanaka; Shin-Ichiro Inoue; Aeri Chung; Tomokazu Ushijima; Tomonao Matsushita; Toshinori Kinoshita; Masato Nakai; Masamitsu Wada; Noriyuki Suetsugu; Eiji Gotoh
  Plant physiology, Mar. 2020, [Reviewed], ［International magazine］
  Blue-light-induced chloroplasts movements play an important role in maximizing light utilization for photosynthesis in plants. Under a weak light condition, chloroplasts accumulate to the cell surface to capture light efficiently (chloroplast accumulation response). Conversely, chloroplasts escape from strong light and move to the side wall to reduce photodamage (chloroplast avoidance response). The blue light receptor phototropin (phot) regulates these chloroplast movements and optimizes leaf photosynthesis by controlling other responses as well as chloroplast movements. Seed plants such as Arabidopsis thaliana have the phototropins phot1 and phot2. They redundantly mediate phototropism, stomatal opening, leaf flattening, and the chloroplast accumulation response. However, the chloroplast avoidance response is induced by strong blue light and regulated primarily by phot2. Phototropins are localized mainly on the plasma membrane. However, a substantial amount of phot2 resides on the chloroplast outer envelope. Therefore, differentially localized phot2 might have different functions. To determine the functions of plasma membrane- and chloroplast envelope-localized phot2, we tethered it to these structures with their respective targeting signals. Plasma membrane-localized phot2 regulated phototropism, leaf flattening, stomatal opening, and chloroplast movements. Chloroplast envelope-localized phot2 failed to mediate phototropism, leaf flattening, and the chloroplast accumulation response but partially regulated the chloroplast avoidance response and stomatal opening. Based on the present and previous findings, we propose that phot2 localized at the interface between the plasma membrane and the chloroplasts is required for the chloroplast avoidance response and possibly for stomatal opening as well.
  English
  DOI：https://doi.org/10.1104/pp.20.00059
  DOI ID：10.1104/pp.20.00059, PubMed ID：32193212
• Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation in Arabidopsis.　　　　　　　　　　　　　　　
  Maki Hayashi; Hodaka Sugimoto; Hirotaka Takahashi; Motoaki Seki; Kazuo Shinozaki; Tatsuya Sawasaki; Toshinori Kinoshita; Shin-Ichiro Inoue
  Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, Volume：19, Number：1, First page：88, Last page：98, Jan. 2020, [Reviewed], [Corresponding], ［International magazine］
  Stomatal pores, which are surrounded by pairs of guard cells in the plant epidermis, regulate gas exchange between plants and the atmosphere, thereby controlling photosynthesis and transpiration. Blue light works as a signal to guard cells, to induce intracellular signaling and open stomata. Blue light receptor phototropins (phots) are activated by blue light; phot-mediated signals promote plasma membrane (PM) H+-ATPase activity via C-terminal Thr phosphorylation, serving as the driving force for stomatal opening in guard cells. However, the details of this signaling process are not fully understood. In this study, through an in vitro screening of phot-interacting protein kinases, we obtained the CBC1 and CBC2 that had been reported as signal transducers in stomatal opening. Promoter activities of CBC1 and CBC2 indicated that both genes were expressed in guard cells. Single and double knockout mutants of CBC1 and CBC2 showed no lesions in the context of phot-mediated phototropism, chloroplast movement, or leaf flattening. In contrast, the cbc1cbc2 double mutant showed larger stomatal opening under both dark and blue light conditions. Interestingly, the level of phosphorylation of C-terminal Thr of PM H+-ATPase was higher in double mutant guard cells. The larger stomatal openings of the double mutant were effectively suppressed by the phytohormone abscisic acid (ABA). CBC1 and CBC2 interacted with BLUS1 and PM H+-ATPase in vitro. From these results, we conclude that CBC1 and CBC2 act as negative regulators of stomatal opening, probably via inhibition of PM H+-ATPase activity.
  English, Scientific journal
  DOI：https://doi.org/10.1039/c9pp00329k
  DOI ID：10.1039/c9pp00329k, PubMed ID：31904040
• Brassinosteroid Induces Phosphorylation of the Plasma Membrane H+-ATPase during Hypocotyl Elongation in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  Anzu Minami; Koji Takahashi; Shin-Ichiro Inoue; Yasuomi Tada; Toshinori Kinoshita
  Plant & cell physiology, Volume：60, Number：5, First page：935, Last page：944, May 2019, [Reviewed], ［Domestic magazine］
  Brassinosteroids (BRs) are steroid phytohormones that regulate plant growth and development, and promote cell elongation at least in part via the acid-growth process. BRs have been suggested to induce cell elongation by the activating plasma membrane (PM) H+-ATPase. However, the mechanism by which BRs activate PM H+-ATPase has not been clarified. In this study, we investigated the effects of BR on hypocotyl elongation and the phosphorylation status of a penultimate residue, threonine, of PM H+-ATPase, which affects the activation, in the etiolated seedlings of Arabidopsis thaliana. Brassinolide (BL), an active endogenous BR, induced hypocotyl elongation, phosphorylation of the penultimate, threonine residue of PM H+-ATPase, and binding of the 14-3-3 protein to PM H+-ATPase in the endogenous BR-depleted seedlings. Changes in both BL-induced elongation and phosphorylation of PM H+-ATPase showed similar concentration dependency. BL did not induce phosphorylation of PM H+-ATPase in the BR receptor mutant bri1-6. In contrast, bikinin, a specific inhibitor of BIN2 that acts as a negative regulator of BR signaling, induced its phosphorylation. Furthermore, BL accumulated the transcripts of SMALL AUXIN UP RNA 9 (SAUR9) and SAUR19, which suppress dephosphorylation of the PM H+-ATPase penultimate residue by inhibiting D-clade type 2C protein phosphatase in the hypocotyls of etiolated seedlings. From these results, we conclude that BL-induced phosphorylation of PM H+-ATPase penultimate residue is mediated via the BRI1-BIN2 signaling pathway, together with the accumulation of SAURs during hypocotyl elongation.
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcz005
  DOI ID：10.1093/pcp/pcz005, PubMed ID：30649552
• Excess Pyrophosphate within Guard Cells Delays Stomatal Closure　　　　　　　　　　　　　　　
  Asaoka M; Inoue S; Gunji S; Kinoshita T; Maeshima M
  Plant & Cell Physiology, Volume：60, Number：4, First page：875, Last page：887, Apr. 2019, [Reviewed], ［Domestic magazine］
  A variety of cellular metabolic reactions generate inorganic pyrophosphate (PPi) as an ATP hydrolysis byproduct. The vacuolar H+-translocating pyrophosphatase (H+-PPase) loss-of-function fugu5 mutant is susceptible to drought and displays pleotropic postgerminative growth defects due to excess PPi. It was recently reported that stomatal closure after abscisic acid (ABA) treatment is delayed in vhp1-1, a fugu5 allele. In contrast, we found that specific removal of PPi rescued all of the above fugu5 developmental and growth defects. Hence, we speculated that excess PPi itself, rather than vacuolar acidification, might delay stomatal closure. To test this hypothesis, we constructed transgenic plants expressing the yeast IPP1 gene (encoding a cytosolic pyrophosphatase) driven by a guard cell-specific promoter (pGC1::IPP1) in the fugu5 background. Our measurements confirmed stomatal closure defects in fugu5, further supporting a role for H+-PPase in stomatal functioning. Importantly, while pGC1::IPP1 transgenics morphologically mimicked fugu5, stomatal closure was restored in response to ABA and darkness. Quantification of water loss revealed that fugu5 stomata were almost completely insensitive to ABA. In addition, growth of pGC1::IPP1 plants was promoted compared to fugu5 throughout their life; however, it did not reach the wild type level. fugu5 also displayed an increased stomatal index, in violation of the one-cell-spacing rule, and phenotypes recovered upon removal of PPi by pAVP1::IPP1 (FUGU5, VHP1 and AVP1 are the same gene encoding H+-PPase), but not in the pGC1::IPP1 line. Taken together, these results clearly support our hypothesis that dysfunction in stomata is triggered by excess PPi within guard cells, probably via perturbed guard cell metabolism.
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcz002
  DOI ID：10.1093/pcp/pcz002, PubMed ID：30649470
• Stomatal response to blue light in crassulacean acid metabolism plants Kalanchoe pinnata and Kalanchoe daigremontiana　　　　　　　　　　　　　　　
  Gotoh E; Oiwamoto K; Inoue S; Shimazaki K; Doi M
  Journal of Experimental Botany, Volume：70, Number：4, First page：1367, Last page：1374, Jan. 2019, [Reviewed], [Corresponding]
  English, Scientific journal
• Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.　　　　　　　　　　　　　　　
  John L Bowman; Takayuki Kohchi; Katsuyuki T Yamato; Jerry Jenkins; Shengqiang Shu; Kimitsune Ishizaki; Shohei Yamaoka; Ryuichi Nishihama; Yasukazu Nakamura; Frédéric Berger; Catherine Adam; Shiori Sugamata Aki; Felix Althoff; Takashi Araki; Mario A Arteaga-Vazquez; Sureshkumar Balasubrmanian; Kerrie Barry; Diane Bauer; Christian R Boehm; Liam Briginshaw; Juan Caballero-Perez; Bruno Catarino; Feng Chen; Shota Chiyoda; Mansi Chovatia; Kevin M Davies; Mihails Delmans; Taku Demura; Tom Dierschke; Liam Dolan; Ana E Dorantes-Acosta; D Magnus Eklund; Stevie N Florent; Eduardo Flores-Sandoval; Asao Fujiyama; Hideya Fukuzawa; Bence Galik; Daniel Grimanelli; Jane Grimwood; Ueli Grossniklaus; Takahiro Hamada; Jim Haseloff; Alexander J Hetherington; Asuka Higo; Yuki Hirakawa; Hope N Hundley; Yoko Ikeda; Keisuke Inoue; Shin-Ichiro Inoue; Sakiko Ishida; Qidong Jia; Mitsuru Kakita; Takehiko Kanazawa; Yosuke Kawai; Tomokazu Kawashima; Megan Kennedy; Keita Kinose; Toshinori Kinoshita; Yuji Kohara; Eri Koide; Kenji Komatsu; Sarah Kopischke; Minoru Kubo; Junko Kyozuka; Ulf Lagercrantz; Shih-Shun Lin; Erika Lindquist; Anna M Lipzen; Chia-Wei Lu; Efraín De Luna; Robert A Martienssen; Naoki Minamino; Masaharu Mizutani; Miya Mizutani; Nobuyoshi Mochizuki; Isabel Monte; Rebecca Mosher; Hideki Nagasaki; Hirofumi Nakagami; Satoshi Naramoto; Kazuhiko Nishitani; Misato Ohtani; Takashi Okamoto; Masaki Okumura; Jeremy Phillips; Bernardo Pollak; Anke Reinders; Moritz Rövekamp; Ryosuke Sano; Shinichiro Sawa; Marc W Schmid; Makoto Shirakawa; Roberto Solano; Alexander Spunde; Noriyuki Suetsugu; Sumio Sugano; Akifumi Sugiyama; Rui Sun; Yutaka Suzuki; Mizuki Takenaka; Daisuke Takezawa; Hirokazu Tomogane; Masayuki Tsuzuki; Takashi Ueda; Masaaki Umeda; John M Ward; Yuichiro Watanabe; Kazufumi Yazaki; Ryusuke Yokoyama; Yoshihiro Yoshitake; Izumi Yotsui; Sabine Zachgo; Jeremy Schmutz
  Cell, Volume：171, Number：2, First page：287, Last page：304, Oct. 2017, [Reviewed], ［International magazine］
  The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cell.2017.09.030
  DOI ID：10.1016/j.cell.2017.09.030, PubMed ID：28985561
• Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1　　　　　　　　　　　　　　　
  Jan Petersen; Shin-ichiro Inoue; Sharon M. Kelly; Stuart Sullivan; Toshinori Kinoshita; John M. Christie
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：292, Number：33, First page：13843, Last page：13852, Aug. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.M117.799643
  DOI ID：10.1074/jbc.M117.799643, ISSN：0021-9258, eISSN：1083-351X, Web of Science ID：WOS:000407942400022
• Blue Light Regulation of Stomatal Opening and the Plasma Membrane H+-ATPase　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Toshinori Kinoshita
  PLANT PHYSIOLOGY, Volume：174, Number：2, First page：531, Last page：538, Jun. 2017, [Reviewed], [Invited], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.17.00166
  DOI ID：10.1104/pp.17.00166, ISSN：0032-0889, eISSN：1532-2548, Web of Science ID：WOS:000403152200007
• Brassinosteroid Involvement in Arabidopsis thaliana Stomatal Opening　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Nozomi Iwashita; Yohei Takahashi; Eiji Gotoh; Eiji Okuma; Maki Hayashi; Ryohei Tabata; Atsushi Takemiya; Yoshiyuki Murata; Michio Doi; Toshinori Kinoshita; Ken-ichiro Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：58, Number：6, First page：1048, Last page：1058, Jun. 2017, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcx049
  DOI ID：10.1093/pcp/pcx049, ISSN：0032-0781, eISSN：1471-9053, Web of Science ID：WOS:000404127900007
• A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening　　　　　　　　　　　　　　　
  Maki Hayashi; Shin-ichiro Inoue; Yoshihisa Ueno; Toshinori Kinoshita
  SCIENTIFIC REPORTS, Volume：7, First page：45586, Mar. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep45586
  DOI ID：10.1038/srep45586, ISSN：2045-2322, Web of Science ID：WOS:000398950000001
• Auxin Influx Carrier AUX1 Confers Acid Resistance for Arabidopsis Root Elongation Through the Regulation of Plasma Membrane H+-ATPase　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Koji Takahashi; Hiromi Okumura-Noda; Toshinori Kinoshita
  PLANT AND CELL PHYSIOLOGY, Volume：57, Number：10, First page：2194, Last page：2201, Oct. 2016, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcw136
  DOI ID：10.1093/pcp/pcw136, ISSN：0032-0781, eISSN：1471-9053, Web of Science ID：WOS:000388374100015
• Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation　　　　　　　　　　　　　　　
  Masaki Okumura; Shin-ichiro Inoue; Keiko Kuwata; Toshinori Kinoshita
  Plant Physiology, Volume：171, Number：1, First page：580, Last page：589, May 2016, [Reviewed]
  American Society of Plant Biologists, English, Scientific journal
  DOI：https://doi.org/10.1104/pp.16.00355
  DOI ID：10.1104/pp.16.00355, ISSN：1532-2548, PubMed ID：27016447, SCOPUS ID：84964886710
• GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement　　　　　　　　　　　　　　　
  Yukari Nagatoshi; Nobutaka Mitsuda; Maki Hayashi; Shin-ichiro Inoue; Eiji Okuma; Akihiro Kubo; Yoshiyuki Murata; Mitsunori Seo; Hikaru Saji; Toshinori Kinoshita; Masaru Ohme-Takagi
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：113, Number：15, First page：4218, Last page：4223, Apr. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1513093113
  DOI ID：10.1073/pnas.1513093113, ISSN：0027-8424, ORCID：45477106, Web of Science ID：WOS:000373762400077
• A Flowering Integrator, SOC1, Affects Stomatal Opening in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Yuriko Kimura; Saya Aoki; Eigo Ando; Ayaka Kitatsuji; Aiko Watanabe; Masato Ohnishi; Koji Takahashi; Shin-ichiro Inoue; Norihito Nakamichi; Yosuke Tamada; Toshinori Kinoshita
  PLANT AND CELL PHYSIOLOGY, Volume：56, Number：4, First page：640, Last page：649, Apr. 2015, [Reviewed], [Invited]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcu214
  DOI ID：10.1093/pcp/pcu214, ISSN：0032-0781, eISSN：1471-9053, Web of Science ID：WOS:000354730200007
• In vitro Phosphorylation Assay of Putative Blue-Light Receptor Phototropins Using Microsomal and Plasma-membrane Fractions Prepared from Vallisneria Leaves　　　　　　　　　　　　　　　
  Yuuki Sakai; Shin-ichiro Inoue; Shingo Takagi
  BIO-PROTOCOL, Volume：5, Number：21, 2015, [Invited]
  Bio-Protocol, LLC, Scientific journal
  DOI：https://doi.org/10.21769/bioprotoc.1647
  DOI ID：10.21769/bioprotoc.1647, ISSN：2331-8325
• Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells　　　　　　　　　　　　　　　
  Yuuki Sakai; Shin-Ichiro Inoue; Akiko Harada; Ken-Ichiro Shimazaki; Shingo Takagi
  JOURNAL OF INTEGRATIVE PLANT BIOLOGY, Volume：57, Number：1, First page：93, Last page：105, Jan. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/jipb.12284
  DOI ID：10.1111/jipb.12284, ISSN：1672-9072, eISSN：1744-7909, Web of Science ID：WOS:000347413600010
• Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Masakazu Tomiyama; Shin-ichiro Inoue; Tomo Tsuzuki; Midori Soda; Sayuri Morimoto; Yukiko Okigaki; Takaya Ohishi; Nobuyoshi Mochizuki; Koji Takahashi; Toshinori Kinoshita
  JOURNAL OF PLANT RESEARCH, Volume：127, Number：4, First page：553, Last page：563, Jul. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-014-0636-0
  DOI ID：10.1007/s10265-014-0636-0, ISSN：0918-9440, eISSN：1618-0860, Web of Science ID：WOS:000338337900009
• Abscisic Acid Suppresses Hypocotyl Elongation by Dephosphorylating Plasma Membrane H+-ATPase in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Yuki Hayashi; Koji Takahashi; Shin-ichiro Inoue; Toshinori Kinoshita
  PLANT AND CELL PHYSIOLOGY, Volume：55, Number：4, First page：845, Last page：853, Apr. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcu028
  DOI ID：10.1093/pcp/pcu028, ISSN：0032-0781, eISSN：1471-9053, Web of Science ID：WOS:000334679500018
• Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth　　　　　　　　　　　　　　　
  Yin Wang; Ko Noguchi; Natsuko Ono; Shin-ichiro Inoue; Ichiro Terashima; Toshinori Kinoshita
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：111, Number：1, First page：533, Last page：538, Jan. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1305438111
  DOI ID：10.1073/pnas.1305438111, ISSN：0027-8424, Web of Science ID：WOS:000329350700119
• Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Tomo Tsuzuki; Koji Takahashi; Masakazu Tomiyama; Shin-Ichiro Inoue; Toshinori Kinoshita
  Frontiers in Plant Science, Volume：4, First page：440, Oct. 2013, [Reviewed]
  Frontiers Research Foundation, English, Scientific journal
  DOI：https://doi.org/10.3389/fpls.2013.00440
  DOI ID：10.3389/fpls.2013.00440, ISSN：1664-462X, SCOPUS ID：84898909058
• TWIN SISTER OF FT, GIGANTEA, and CONSTANS Have a Positive But Indirect Effect on Blue Light-Induced Stomatal Opening in Arabidopsis　　　　　　　　　　　　　　　
  Eigo Ando; Masato Ohnishi; Yin Wang; Tomonao Matsushita; Aiko Watanabe; Yuki Hayashi; Miho Fujii; Jian Feng Ma; Shin-ichiro Inoue; Toshinori Kinoshita
  PLANT PHYSIOLOGY, Volume：162, Number：3, First page：1529, Last page：1538, Jul. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.113.217984
  DOI ID：10.1104/pp.113.217984, ISSN：0032-0889, eISSN：1532-2548, Web of Science ID：WOS:000321325700024
• Role of RPT2 in Leaf Positioning and Flattening and a Possible Inhibition of phot2 Signaling by phot1　　　　　　　　　　　　　　　
  Akiko Harada; Atsushi Takemiya; Shin-ichiro Inoue; Tatsuya Sakai; Ken-ichiro Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：54, Number：1, First page：36, Last page：47, Jan. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcs094
  DOI ID：10.1093/pcp/pcs094, ISSN：0032-0781, Web of Science ID：WOS:000315218100004
• Characterization of the Plasma Membrane H+-ATPase in the Liverwort Marchantia polymorpha　　　　　　　　　　　　　　　
  Masaki Okumura; Shin-ichiro Inoue; Koji Takahashi; Kimitsune Ishizaki; Takayuki Kohchi; Toshinori Kinoshita
  PLANT PHYSIOLOGY, Volume：159, Number：2, First page：826, Last page：+, Jun. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.112.195537
  DOI ID：10.1104/pp.112.195537, ISSN：0032-0889, Web of Science ID：WOS:000304834800024
• Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte　　　　　　　　　　　　　　　
  Masaki Okumura; Koji Takahashi; Shin-ichiro Inoue; Toshinori Kinoshita
  Plant Signaling and Behavior, Volume：7, Number：8, First page：979-982, 2012, [Reviewed], [Invited]
  Landes Bioscience, English, Scientific journal
  DOI：https://doi.org/10.4161/psb.20936
  DOI ID：10.4161/psb.20936, ISSN：1559-2324, PubMed ID：22836495, SCOPUS ID：84864819460
• FLOWERING LOCUS T Regulates Stomatal Opening　　　　　　　　　　　　　　　
  Toshinori Kinoshita; Natsuko Ono; Yuki Hayashi; Sayuri Morimoto; Suguru Nakamura; Midori Soda; Yuma Kato; Masato Ohnishi; Takeshi Nakano; Shin-ichiro Inoue; Ken-ichiro Shimazaki
  CURRENT BIOLOGY, Volume：21, Number：14, First page：1232, Last page：1238, Jul. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2011.06.025
  DOI ID：10.1016/j.cub.2011.06.025, ISSN：0960-9822, eISSN：1879-0445, Web of Science ID：WOS:000293320000026
• Immunohistochemical Detection of Blue Light-Induced Phosphorylation of the Plasma Membrane H+-ATPase in Stomatal Guard Cells　　　　　　　　　　　　　　　
  Maki Hayashi; Shin-ichiro Inoue; Koji Takahashi; Toshinori Kinoshita
  PLANT AND CELL PHYSIOLOGY, Volume：52, Number：7, First page：1238, Last page：1248, Jul. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcr072
  DOI ID：10.1093/pcp/pcr072, ISSN：0032-0781, eISSN：1471-9053, Web of Science ID：WOS:000292837500010
• Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Tomo Tsuzuki; Koji Takahashi; Shin-ichiro Inoue; Yukiko Okigaki; Masakazu Tomiyama; Mohammad Anowar Hossain; Ken-ichiro Shimazaki; Yoshiyuki Murata; Toshinori Kinoshita
  JOURNAL OF PLANT RESEARCH, Volume：124, Number：4, First page：527, Last page：538, Jul. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-011-0426-x
  DOI ID：10.1007/s10265-011-0426-x, ISSN：0918-9440, eISSN：1618-0860, Web of Science ID：WOS:000292448700009
• Functional Analyses of the Activation Loop of Phototropin2 in Arabidopsis　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Tomonao Matsushita; Yuta Tomokiyo; Masaki Matsumoto; Keiichi I. Nakayama; Toshinori Kinoshita; Ken-ichiro Shimazaki
  PLANT PHYSIOLOGY, Volume：156, Number：1, First page：117, Last page：128, May 2011, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.111.175943
  DOI ID：10.1104/pp.111.175943, ISSN：0032-0889, Web of Science ID：WOS:000290207100010
• Phototropin signaling and stomatal opening as a model case　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Atsushi Takemiya; Ken-ichiro Shimazaki
  CURRENT OPINION IN PLANT BIOLOGY, Volume：13, Number：5, First page：587, Last page：593, Oct. 2010, [Reviewed], [Invited], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.pbi.2010.09.002
  DOI ID：10.1016/j.pbi.2010.09.002, ISSN：1369-5266, Web of Science ID：WOS:000284658400016
• The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 Protein Is a Phototropin Signaling Element That Regulates Leaf Flattening and Leaf Positioning　　　　　　　　　　　　　　　
  Matthieu de Carbonnel; Phillip Davis; M. Rob G. Roelfsema; Shin-ichiro Inoue; Isabelle Schepens; Patricia Lariguet; Markus Geisler; Ken-ichiro Shimazaki; Roger Hangarter; Christian Fankhauser
  PLANT PHYSIOLOGY, Volume：152, Number：3, First page：1391, Last page：1405, Mar. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.109.150441
  DOI ID：10.1104/pp.109.150441, ISSN：0032-0889, Web of Science ID：WOS:000276329500023
• Blue light-induced autophosphorylation of phototropin is a primary step for signaling　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Toshinori Kinoshita; Masaki Matsumoto; Keiichi I. Nakayama; Michio Doi; Ken-ichiro Shimazaki
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：105, Number：14, First page：5626, Last page：5631, Apr. 2008, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.0709189105
  DOI ID：10.1073/pnas.0709189105, ISSN：0027-8424, Web of Science ID：WOS:000254893600061
• Leaf positioning of Arabidopsis in response to blue light　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Toshinori Kinoshita; Atsushi Takemiya; Michio Doi; Ken-ichiro Shimazaki
  MOLECULAR PLANT, Volume：1, Number：1, First page：15, Last page：26, Jan. 2008, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1093/mp/ssm001
  DOI ID：10.1093/mp/ssm001, ISSN：1674-2052, Web of Science ID：WOS:000259068900004
• Possible involvement of phototropins in leaf movement of kidney bean in response to blue light　　　　　　　　　　　　　　　
  S Inoue; T Kinoshita; K Shimazaki
  PLANT PHYSIOLOGY, Volume：138, Number：4, First page：1994, Last page：2004, Aug. 2005, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.105.062026
  DOI ID：10.1104/pp.105.062026, ISSN：0032-0889, Web of Science ID：WOS:000231206600018
• Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light　　　　　　　　　　　　　　　
  K Ueno; T Kinoshita; S Inoue; T Emi; K Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：46, Number：6, First page：955, Last page：963, Jun. 2005, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pci104
  DOI ID：10.1093/pcp/pci104, ISSN：0032-0781, Web of Science ID：WOS:000230090400015
• Phototropins promote plant growth in response to blue light in low light environments　　　　　　　　　　　　　　　
  A Takemiya; S Inoue; M Doi; T Kinoshita; K Shimazaki
  PLANT CELL, Volume：17, Number：4, First page：1120, Last page：1127, Apr. 2005, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.104.030049
  DOI ID：10.1105/tpc.104.030049, ISSN：1040-4651, eISSN：1532-298X, Web of Science ID：WOS:000228375900008

• シロイヌナズナにおける液胞へのマグネシウム輸送調節機構の解明　　　　　　　　　　　　　　　
  井上晋一郎; 林真妃; 黄勝; 横正健剛; 奥村将樹; 木下俊則; 木下俊則; 馬建鋒
  Volume：69, 2023
  ISSN：2424-0575, J-Global ID：202402270862154016
• 植物のMgホメオスタシス維持を調節するイオン輸送体とシグナル伝達機構の解明　　　　　　　　　　　　　　　
  井上晋一郎; 林真妃; HUANG Sheng; 横正健剛; 後藤栄治; 池松朱夏; 奥村将樹; 鈴木孝征; 木下俊則; 木下俊則; 馬建鋒
  Volume：17th (Web), 2023
  J-Global ID：202302232810339529
• 植物の光合成と蒸散を調節するMgホメオスタシス維持機構　　　　　　　　　　　　　　　
  井上晋一郎; 林真妃; 奥村将樹; 後藤栄治; 横正健剛; 馬建鋒; 馬建鋒; 木下俊則
  Volume：66, 2020
  ISSN：2424-0575, J-Global ID：202002261207887106
• オオセキショウモにおける青色光受容体フォトトロピンの同定と解析　　　　　　　　　　　　　　　
  酒井友希; 井上晋一郎; 原田明子; 島崎研一郎; 高木慎吾
  Volume：73rd, 2009
  J-Global ID：200902253834692241
• Blue light mediates normal leaf positioning in Arabidopsis　　　　　　　　　　　　　　　
  Shin-ichiro Inoue; Toshinori Kinoshita; Atsushi Takemiya; Michio Doi; Ken-ichiro Shirnazaki
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S233, Last page：S233, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922701408
• Phototropins may mediate leaf movement of kidney bean in response to blue light　　　　　　　　　　　　　　　
  S Inoue; T Kinoshita; K Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S207, Last page：S207, 2006
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000236401401313
• Characterization of the 14-3-3 protein binding to phot1 in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  S Inoue; T Kinoshita; K Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S167, Last page：S167, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101172
• Phototropins promote plant growth in low light environment　　　　　　　　　　　　　　　
  A Takemiya; S Inoue; M Doi; T Kinoshita; K Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S86, Last page：S86, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104100342
• Blue light-dependent binding of a 14-3-3 protein to phot1 in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  S Inoue; T Kinoshita; K Shimazaki
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S84, Last page：S84, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700333

• 化学と生物　　　　　　　　　　　　　　　
  井上 晋一郎, [Single work]
  Oct. 2024
• アグリバイオ　　　　　　　　　　　　　　　
  井上 晋一郎, [Single work]
  2023

• Jul. 2024 - Present
  Regulatory Biology, Saitama University
• Jun. 2024 - Present
  Laboratory Course in Regulatory Biology - Basic, Saitama University
• Apr. 2024 - Present
  Seminar of Adaptational Morphology, Saitama University
• Apr. 2024 - Present
  Seminar in Plant Physiology, Saitama University
• Apr. 2024 - Present
  Laboratory Course in Regulatory Biology, Saitama University
• 2021 - Present
  Seminars in Basic Biological Science I, Nagoya University
• 2022 - 2022
  modern life science, Nagoya University

• May 2023 - Present, Japan Transporter Research Association
• Sep. 2021 - Present
• 2020 - Present
• Sep. 2005 - Present
• Dec. 2002 - Present

• マグネシウムイオンによる新奇気孔開口誘導機構の解明　　　　　　　　　　　　　　　
  Nov. 2022 - Oct. 2024
  Principal investigator
• 植物の環境応答を調節する新規マグネシウム輸送体の同定と輸送制御機構の解明　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2024
  Grant amount(Total)：5720000, Direct funding：4400000, Indirect funding：1320000
  Grant number：22H04805
• 気孔の開口を駆動する細胞膜プロトンポンプの活性調節機構の解明　　　　　　　　　　　　　　　
  01 Apr. 2020 - 31 Mar. 2023
  Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
  Grant number：20K06703
• Identification and functional characterization of protein kinases mediating blue light-dependent stomatal opening　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2015 - 31 Mar. 2018
  Inoue Shin-ichiro; HAYASHI Maki; OKUMURA Masaki, Nagoya University
  Grant amount(Total)：5200000, Direct funding：4000000, Indirect funding：1200000
  Stomata open in response to blue light, and the opening is mediated by a phototropin-activated proton pump and a consequent potassium uptake in stomatal guard cells. However, signaling for the stomatal opening is not fully understood. In this study, we performed a protein-protein interaction screening and identified CIPK23 as a phototropin interactor. As the results of functional analyses of CIPK23, we found that CIPK23 mediates stomatal opening not through the proton pump activation but through the potassium channel activation in guard cells.
  Grant number：15K07101
• Stomatal response in crassulacean acid metabolism plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2014 - 31 Mar. 2017
  Doi Michio, Kyushu University
  Grant amount(Total)：4940000, Direct funding：3800000, Indirect funding：1140000
  We investigated stomatal blue light (BL) response in obligate crassulacean acid metabolism (CAM) plants, Kalanchoe pinnata and Kalanchoe daigremontiana. The stomatal responses to BL were determined in both intact leaves and detached epidermis using dual-beam protocol to distinguish the BL-dependent stomatal opening from the photosynthesis-dependent stomatal opening. The stomata open in response to BL superimposed on the background red light (RL). The BL-dependent stomatal opening was completely inhibited by both an inhibitor of type 1 protein phosphatase and an inhibitor of the plasma membrane H+-ATPase. A fungous toxin fusicoccin (FC), an activator of the plasma membrane H+-ATPase induced stomatal opening in the dark. BL and FC caused both activation of the plasma membrane H+-ATPase in guard cells, and stomatal opening.
  These results indicate that BL-dependent stomatal opening operates in obligate CAM plants, regardless of the difference in the modes of photosynthesis.
  Grant number：26440150
• Functional analysis of protein kinase that interacts with blue light-receptor phototropin in stomatal opening　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), 01 Apr. 2013 - 31 Mar. 2015
  INOUE Shin-ichiro, Nagoya University
  Grant amount(Total)：4550000, Direct funding：3500000, Indirect funding：1050000
  Stomata in the plant epidermis open in response to blue light and contribute to plant growth by regulating photosynthesis and transpiration. A blue light-receptor phototropin mediates the stomatal opening via promotion of an active transport of K+ in stomatal guard cells. However, signaling for the stomatal opening is not fully understood. In this study, we performed functional analysis of a protein kinase that interacts with phototropin, and characterized roles of the protein kinase in the blue light signaling for stomatal opening.
  Grant number：25840105
• Molecular mechanism of stomatal movements in response to the environmental stimuli　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 01 Apr. 2010 - 31 Mar. 2015
  KINOSHITA Toshinori; TAKAHASHI Koji; INOUE Shin-ichiro, Nagoya University
  Grant amount(Total)：84110000, Direct funding：64700000, Indirect funding：19410000
  Stomata in the plant epidermis regulate gas exchange between plants and atmosphere. Stomatal aperture is regulates numerous environmental signals. Opening of stomata induces uptake of CO2 for photosynthesis and transpiration. In this study, we investigated signaling pathways for stomatal opening and closing by genetic, physiological, and biochemical methods. In addition, we examined effect of stomatal aperture in the transgenic plants on plant growth and drought resistance.
  Grant number：22119005
• The transduction of light signal to ion transport in stomatal guard cells.　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), 01 Apr. 2009 - 31 Mar. 2015
  SHIMAZAKI Ken-ichiro; KINOSHITA Toshinori; TAKEMIYA Atsushi; INOUE Shin-ichiro; TAKAHASHI Yohei, Kyushu University
  Grant amount(Total)：205920000, Direct funding：158400000, Indirect funding：47520000
  We studied phototropin-mediated signaling components in guard cells as a model case for plant light signaling. We showed that phosphatidic acid, a phospholipid second messenger produced by ABA, inhibited protein phosphatase 1 (PP1), a positive regulator of stomatal blue light signaling. The result suggests that PP1 is a crosstalk point between blue light and ABA signaling. We identified autophosphorylation sites in phot2 by mass analysis and showed the phosphorylation of Ser-761 and Ser-763 is required for the signaling. We found a novel protein kinase named BLUS1 as a substrate for phototropins and demonstrated phosphorylation of Ser-348 in response to blue light. We demonstrated that the phosphorylation of Ser-348 is essential for the signaling and that the Ser-348 is directly phosphorylated by phototropins. We showed the expression of 11 isoforms of the H+-ATPases in guard cells and identified the isoform responsible for blue light-dependent stomatal opening as AHA1.
  Grant number：21227001
• 気孔の開口を駆動する細胞膜プロトンポンプの活性制御機構の解明　　　　　　　　　　　　　　　
  2010 - 2012
  Grant amount(Total)：5000000, Direct funding：5000000
  Grant number：10J00254
• 青色光受容体フォトトロピンのリン酸化情報伝達機構の解明　　　　　　　　　　　　　　　
  2009 - 2010
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  Grant number：21770052