Evolution and divergence of the role of plant ethylene receptor–related histidine kinases in abscisic acid signaling Marcos T. Miyabe; Taketo Sasaki; Tsukasa Toriyama; Rahul Sk; Daisuke Takezawa; Izumi Yotsui; Teruaki Taji; Yoichi Sakata
Biochemical and Biophysical Research Communications,
Volume：747,
First page：151295,
Last page：151295, Feb. 2025,
[Reviewed]Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.bbrc.2025.151295DOI ID：10.1016/j.bbrc.2025.151295,
ISSN：0006-291X Targeted in vivo mutagenesis of a sensor histidine kinase playing an essential role in ABA signaling of the moss Physcomitrium patens Rahul Sk; Marcos Takeshi Miyabe; Daisuke Takezawa; Shunsuke Yajima; Izumi Yotsui; Teruaki Taji; Yoichi Sakata
Biochemical and Biophysical Research Communications,
Volume：637,
First page：93,
Last page：99, Dec. 2022,
[Reviewed]Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.bbrc.2022.11.009DOI ID：10.1016/j.bbrc.2022.11.009,
ISSN：0006-291X Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverwortsAkida Jahan; Yuto Yamazaki; Mousona Islam; Totan Kumar Ghosh; Nami Yoshimura; Hirotaka Kato; Kimitsune Ishizaki; Akihisa Shinozawa; Yoichi Sakata; Daisuke Takezawa
Frontiers in Plant Science,
Volume：13, Jul. 2022,
[Reviewed]Phytohormone abscisic acid (ABA) plays a key role in stomata closure, osmostress acclimation, and vegetative and embryonic dormancy. Group B3 Raf protein kinases (B3-Rafs) serve as positive regulators of ABA and osmostress signaling in the moss Physcomitrium patens and the angiosperm Arabidopsis thaliana. While P. patens has a single B3-Raf called ARK, specific members of B3-Rafs among six paralogs regulate ABA and osmostress signaling in A. thaliana, indicating functional diversification of B3-Rafs in angiosperms. However, we found that the liverwort Marchantia polymorpha, belonging to another class of bryophytes, has three paralogs of B3-Rafs, MpARK1, MpARK2, and MpARK3, with structural variations in the regulatory domains of the polypeptides. By reporter assays of the P. patens ark line and analysis of genome-editing lines of M. polymorpha, we found that these B3-Rafs are functionally redundant in ABA response, with respect to inhibition of growth, tolerance to desiccation and expression of stress-associated transcripts, the majority of which are under the control of the PYR/PYL/RCAR-like receptor MpPYL1. Interestingly, gemmae in gemma cups were germinating only in mutant lines associated with MpARK1, indicating that dormancy in the gametophyte is controlled by a specific B3-Raf paralog. These results indicated not only conservation of the role of B3-Rafs in ABA and osmostress response in liverworts but also functional diversification of B3-Rafs, which is likely to have occurred in the early stages of land plant evolution.
Frontiers Media SA, Scientific journal
DOI：https://doi.org/10.3389/fpls.2022.952820DOI ID：10.3389/fpls.2022.952820,
eISSN：1664-462X Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens Tsukasa Toriyama; Akihisa Shinozawa; Yuki Yasumura; Masashi Saruhashi; Mayuka Hiraide; Shiori Ito; Hideyuki Matsuura; Keiko Kuwata; Mika Yoshida; Tadashi Baba; Izumi Yotsui; Teruaki Taji; Daisuke Takezawa; Yoichi Sakata
Current Biology,
Volume：32,
Number：1,
First page：164,
Last page：175.e8, Jan. 2022,
[Reviewed]Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.cub.2021.10.068DOI ID：10.1016/j.cub.2021.10.068,
ISSN：0960-9822 Activation of SnRK2 by Raf-like kinase ARK represents a primary mechanism of ABA and abiotic stress responsesMousona Islam; Takumi Inoue; Mayuka Hiraide; Nobiza Khatun; Akida Jahan; Keiko Kuwata; Sotaro Katagiri; Taishi Umezawa; Izumi Yotsui; Yoichi Sakata; Daisuke Takezawa
Plant Physiology,
Volume：185,
Number：2,
First page：533,
Last page：546, Dec. 2021,
[Reviewed]Abstract
The Raf-like protein kinase abscisic acid (ABA) and abiotic stress-responsive Raf-like kinase (ARK) previously identified in the moss Physcomitrium (Physcomitrella) patens acts as an upstream regulator of subgroup III SNF1-related protein kinase2 (SnRK2), the key regulator of ABA and abiotic stress responses. However, the mechanisms underlying activation of ARK by ABA and abiotic stress for the regulation of SnRK2, including the role of ABA receptor-associated group A PP2C (PP2C-A), are not understood. We identified Ser1029 as the phosphorylation site in the activation loop of ARK, which provided a possible mechanism for regulation of its activity. Analysis of transgenic P. patens ark lines expressing ARK-GFP with Ser1029-to-Ala mutation indicated that this replacement causes reductions in ABA-induced gene expression, stress tolerance, and SnRK2 activity. Immunoblot analysis using an anti-phosphopeptide antibody indicated that ABA treatments rapidly stimulate Ser1029 phosphorylation in the wild type (WT). The phosphorylation profile of Ser1029 in ABA-hypersensitive ppabi1 lacking protein phosphatase 2C-A (PP2C-A) was similar to that in the WT, whereas little Ser1029 phosphorylation was observed in ABA-insensitive ark missense mutant lines. Furthermore, newly isolated ppabi1 ark lines showed ABA-insensitive phenotypes similar to those of ark lines. Therefore, ARK is a primary activator of SnRK2, preceding negative regulation by PP2C-A in bryophytes, which provides a prototype mechanism for ABA and abiotic stress responses in plants.
Oxford University Press (OUP), Scientific journal
DOI：https://doi.org/10.1093/plphys/kiaa046DOI ID：10.1093/plphys/kiaa046,
ISSN：0032-0889,
eISSN：1532-2548 Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2Yoshiaki Kamiyama; Misaki Hirotani; Shinnosuke Ishikawa; Fuko Minegishi; Sotaro Katagiri; Conner J. Rogan; Fuminori Takahashi; Mika Nomoto; Kazuya Ishikawa; Yutaka Kodama; Yasuomi Tada; Daisuke Takezawa; Jeffrey C. Anderson; Scott C. Peck; Kazuo Shinozaki; Taishi Umezawa
Proceedings of the National Academy of Sciences,
Volume：118,
Number：30,
First page：e2100073118,
Last page：e2100073118, Jul. 2021,
[Reviewed]The phytohormone abscisic acid (ABA) plays a major role in abiotic stress responses in plants, and subclass III SNF1-related protein kinase 2 (SnRK2) kinases mediate ABA signaling. In this study, we identified Raf36, a group C Raf-like protein kinase in
Arabidopsis, as a protein that interacts with multiple SnRK2s. A series of reverse genetic and biochemical analyses revealed that 1) Raf36 negatively regulates ABA responses during postgermination growth, 2) the N terminus of Raf36 is directly phosphorylated by SnRK2s, and 3) Raf36 degradation is enhanced in response to ABA. In addition, Raf22, another C-type Raf-like kinase, functions partially redundantly with Raf36 to regulate ABA responses. A comparative phosphoproteomic analysis of ABA-induced responses of wild-type and
raf22raf36-1 plants identified proteins that are phosphorylated downstream of Raf36 and Raf22 in planta. Together, these results support a model in which Raf36/Raf22 function mainly under optimal conditions to suppress ABA responses, whereas in response to ABA, the SnRK2 module promotes Raf36 degradation as a means of alleviating Raf36-dependent inhibition and allowing for heightened ABA signaling to occur.
Proceedings of the National Academy of Sciences, Scientific journal
DOI：https://doi.org/10.1073/pnas.2100073118DOI ID：10.1073/pnas.2100073118,
ISSN：0027-8424,
eISSN：1091-6490 Arabidopsis Raf‐like kinases act as positive regulators of subclass III SnRK2 in osmostress signalingShohei Katsuta; Goro Masuda; Hyeokjin Bak; Akihisa Shinozawa; Yoshiaki Kamiyama; Taishi Umezawa; Daisuke Takezawa; Izumi Yotsui; Teruaki Taji; Yoichi Sakata
The Plant Journal,
Volume：103,
Number：2,
First page：634,
Last page：644, Jul. 2020,
[Reviewed]Wiley, English, Scientific journal
DOI：https://doi.org/10.1111/tpj.14756DOI ID：10.1111/tpj.14756,
ISSN：0960-7412,
eISSN：1365-313X Phosphoproteomic profiling reveals ABA-responsive phosphosignaling pathways in Physcomitrella patens. Amagai A; Honda Y; Ishikawa S; Hara Y; Kuwamura M; Shinozawa A; Sugiyama N; Ishihama Y; Takezawa D; Sakata Y; Shinozaki K; Umezawa T
The Plant journal : for cell and molecular biology,
Volume：94,
Number：4,
First page：699,
Last page：708, May 2018,
[Reviewed]DOI：https://doi.org/10.1111/tpj.13891DOI ID：10.1111/tpj.13891,
ISSN：0960-7412,
PubMed ID：29575231,
Web of Science ID：WOS:000432036300012 Abscisic acid-induced gene expression in the liverwort Marchantia polymorpha is mediated by evolutionarily conserved promoter elements Totan K. Ghosh; Midori Kaneko; Khaleda Akter; Shuhei Murai; Kenji Komatsu; Kimitsune Ishizaki; Katsuyuki T. Yamato; Takayuki Kohchi; Daisuke Takezawa
PHYSIOLOGIA PLANTARUM,
Volume：156,
Number：4,
First page：407,
Last page：420, Apr. 2016,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1111/ppl.12385DOI ID：10.1111/ppl.12385,
ISSN：0031-9317,
eISSN：1399-3054,
PubMed ID：26456006,
Web of Science ID：WOS:000372974400004 Plant Raf-like kinase integrates abscisic acid and hyperosmotic stress signaling upstream of SNF1-related protein kinase2. Saruhashi M; Kumar Ghosh T; Arai K; Ishizaki Y; Hagiwara K; Komatsu K; Shiwa Y; Izumikawa K; Yoshikawa H; Umezawa T; Sakata Y; Takezawa D
Proceedings of the National Academy of Sciences of the United States of America,
Volume：112,
Number：46,
First page：E6388, Nov. 2015,
[Reviewed]Proceedings of the National Academy of Sciences
DOI：https://doi.org/10.1073/pnas.1511238112DOI ID：10.1073/pnas.1511238112,
ISSN：0027-8424,
ORCID：31588702,
PubMed ID：26540727 Epoxycarotenoid‐mediated synthesis of abscisic acid in Physcomitrella patens implicating conserved mechanisms for acclimation to hyperosmosis in embryophytesDaisuke Takezawa; Naoki Watanabe; Totan Kumar Ghosh; Masashi Saruhashi; Atsushi Suzuki; Kanako Ishiyama; Shinnosuke Somemiya; Masatomo Kobayashi; Yoichi Sakata
New Phytologist,
Volume：206,
Number：1,
First page：209,
Last page：219, Dec. 2014,
[Reviewed]Summary
Plants acclimate to environmental stress signals such as cold, drought and hypersalinity, and provoke internal protective mechanisms. Abscisic acid (ABA), a carotenoid‐derived phytohormone, which increases in response to the stress signals above, has been suggested to play a key role in the acclimation process in angiosperms, but the role of ABA in basal land plants such as mosses, including its biosynthetic pathways, has not been clarified.
Targeted gene disruption of PpABA1, encoding zeaxanthin epoxidase in the moss Physcomitrella patens was conducted to determine the role of endogenous ABA in acclimation processes in mosses.
The generated ppaba1 plants were found to accumulate only a small amount of endogenous ABA. The ppaba1 plants showed reduced osmotic acclimation capacity in correlation with reduced dehydration tolerance and accumulation of late embryogenesis abundant proteins. By contrast, cold‐induced freezing tolerance was less affected in ppaba1, indicating that endogenous ABA does not play a major role in the regulation of cold acclimation in the moss.
Our results suggest that the mechanisms for osmotic acclimation mediated by carotenoid‐derived synthesis of ABA are conserved in embryophytes and that acquisition of the mechanisms played a crucial role in terrestrial adaptation and colonization by land plant ancestors.
Wiley, Scientific journal
DOI：https://doi.org/10.1111/nph.13231DOI ID：10.1111/nph.13231,
ISSN：0028-646X,
eISSN：1469-8137 Biochemical and structural characterization of an endoplasmic reticulum-localized late embryogenesis abundant (LEA) protein from the liverwort Marchantia polymorpha. Hatanaka R; Furuki T; Shimizu T; Takezawa D; Kikawada T; Sakurai M; Sugawara Y; Biochemical; biophysical research communications
Biochemical and biophysical research communications,
Volume：454,
Number：4,
First page：588,
Last page：593, Nov. 2014,
[Reviewed]Hatanaka R, Furuki T, Shimizu T, Takezawa D, Kikawada T, Sakurai M, Sugawara Y, Biochemical and biophysical research communications, 2014, vol. 454, no. 4, pp. 588-593
DOI：https://doi.org/10.1016/j.bbrc.2014.10.130DOI ID：10.1016/j.bbrc.2014.10.130,
ISSN：0006-291X,
ORCID：14673271,
PubMed ID：25450698 Abscisic acid-induced rearrangement of intracellular structures associated with freezing and desiccation stress tolerance in the liverwort Marchantia polymorpha Khaleda Akter; Masahiro Kato; Yuki Sato; Yasuko Kaneko; Daisuke Takezawa
JOURNAL OF PLANT PHYSIOLOGY,
Volume：171,
Number：15,
First page：1334,
Last page：1343, Sep. 2014,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1016/j.jplph.2014.05.004DOI ID：10.1016/j.jplph.2014.05.004,
ISSN：0176-1617,
eISSN：1618-1328,
Web of Science ID：WOS:000345631000003 Group A PP2Cs evolved in land plants as key regulators of intrinsic desiccation tolerance Kenji Komatsu; Norihiro Suzuki; Mayuri Kuwamura; Yuri Nishikawa; Mao Nakatani; Hitomi Ohtawa; Daisuke Takezawa; Motoaki Seki; Maho Tanaka; Teruaki Taji; Takahisa Hayashi; Yoichi Sakata
NATURE COMMUNICATIONS,
Volume：4,
First page：2219, Jul. 2013,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1038/ncomms3219DOI ID：10.1038/ncomms3219,
ISSN：2041-1723,
PubMed ID：23900426,
Web of Science ID：WOS:000323717600002 Cold acclimation in the moss Physcomitrella patens involves abscisic acid-dependent signaling. Bhyan SB; Minami A; Kaneko Y; Suzuki S; Arakawa K; Sakata Y; Takezawa D
Journal of plant physiology,
Volume：169,
Number：2,
First page：137,
Last page：145, Jan. 2012,
[Reviewed]DOI：https://doi.org/10.1016/j.jplph.2011.08.004DOI ID：10.1016/j.jplph.2011.08.004,
ISSN：0176-1617,
PubMed ID：21958596 ABA in bryophytes: how a universal growth regulator in life became a plant hormone? Daisuke Takezawa; Kenji Komatsu; Yoichi Sakata
JOURNAL OF PLANT RESEARCH,
Volume：124,
Number：4,
First page：437,
Last page：453, Jul. 2011,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1007/s10265-011-0410-5DOI ID：10.1007/s10265-011-0410-5,
ISSN：0918-9440,
Web of Science ID：WOS:000292448700002 Endoplasmic reticulum-localized small heat shock protein that accumulates in mulberry tree (Morus bombycis Koidz.) during seasonal cold acclimation is responsive to abscisic acid Norifumi Ukaji; Chikako Kuwabara; Yuri Kanno; Mitsunori Seo; Daisuke Takezawa; Keita Arakawa; Seizo Fujikawa
TREE PHYSIOLOGY,
Volume：30,
Number：4,
First page：502,
Last page：513, Apr. 2010,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1093/treephys/tpp125DOI ID：10.1093/treephys/tpp125,
ISSN：0829-318X,
PubMed ID：20100700,
Web of Science ID：WOS:000276305100007 Evolutionarily conserved regulatory mechanisms of abscisic acid signaling in land plants: characterization of ABSCISIC ACID INSENSITIVE1-like type 2C protein phosphatase in the liverwort Marchantia polymorpha. Tougane K; Komatsu K; Bhyan SB; Sakata Y; Ishizaki K; Yamato KT; Kohchi T; Takezawa D; Plant physiology, v
Volume：152,
Number：3,
First page：1529,
Last page：1543, Mar. 2010,
[Reviewed]Tougane K, Komatsu K, Bhyan SB, Sakata Y, Ishizaki K, Yamato KT, Kohchi T, Takezawa D, Plant physiology, 2010, vol. 152, no. 3, pp. 1529-1543
DOI：https://doi.org/10.1104/pp.110.153387DOI ID：10.1104/pp.110.153387,
ORCID：10384760,
PubMed ID：20097789 Identification of Gene Expressed in Xylem Ray Parenchyma Cells of Larix kaempferi That Adapt to Cold Environment by Deep Supercooling 高田直樹; 南杏鶴; 荒川圭太; 竹沢大輔; 藤川清三
低温生物工学会誌,
Volume：49,
Number：2,
First page：191,
Last page：194, Dec. 2003
Japanese
DOI：https://doi.org/10.20585/cryobolcryotechnol.49.2_191DOI ID：10.20585/cryobolcryotechnol.49.2_191,
ISSN：1340-7902,
J-Global ID：200902245359338125 Physiological Changes Associated with Abscisic Acid-Induced Freezing Tolerance in Physicomitrella patens MINAMI Anzu; NAGAO Manabu; ARAKAWA Keita; FUJIKAWA Seizo; TAKEZAWA Daisuke
Cryobiology and Cryotechnology,
Volume：49,
Number：2,
First page：179,
Last page：183, 2003
The phytohormone abscisic acid (ABA) plays an important role in freezing tolerance in plants. Treatment with ABA increased freezing tolerance of protonema cells of the moss Physcomitrella patens. ABA triggered morphological changes in cellular organelles, such as reduction in sizes of chloroplasts and vacuoles, and physiological changes such as accumulation of soluble sugars, especially that of sucrose, and boiling-stable proteins. We used protein synthesis inhibitors, protein serine/threonine kinase inhibitors and protein serine/threonine phosphatase inhibitors to examine cellular events associated with ABA-induced freezing tolerance. The results indicated that a protein synthesis inhibitor cycloheximide dramatically decreased freezing tolerance of the ABA-treated cells and accumulation of boiling-stable proteins. A phosphatase inhibitor okadaic acid also decreased ABA-induced freezing tolerance in P. patens. These inhibitors, however, did not have any effect on ABA-induced accumulation of sucrose. These results suggest critical roles of de novo synthesis of nuclear encoded protein and phosphatase-mediated signal transduction in ABA-induced cellular processes leading to freezing tolerance, and that sucrose only plays a limited role in these processes.
Japanese Society of Cryobiology and Cryotechnology, Japanese
DOI：https://doi.org/10.20585/cryobolcryotechnol.49.2_179DOI ID：10.20585/cryobolcryotechnol.49.2_179,
ISSN：1340-7902,
J-Global ID：200902284634086862,
CiNii Articles ID：110002914000,
CiNii Books ID：AN10448734 Roles of the plasma membrane and the cell wall in the responses of plant cells to freezing. Yamada T; Kuroda K; Jitsuyama Y; Takezawa D; Arakawa K; Fujikawa S
Planta,
Volume：215,
First page：770,
Last page：778, Sep. 2002,
[Reviewed]5
DOI：https://doi.org/10.1007/s00425-002-0814-5DOI ID：10.1007/s00425-002-0814-5,
ISSN：0032-0935,
PubMed ID：12244442 Abscisic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale C Kuwabara; D Takezawa; T Shimada; T Hamada; S Fujikawa; K Arakawa
PHYSIOLOGIA PLANTARUM,
Volume：115,
Number：1,
First page：101,
Last page：110, May 2002,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1034/j.1399-3054.2002.1150112.xDOI ID：10.1034/j.1399-3054.2002.1150112.x,
ISSN：0031-9317,
PubMed ID：12010473,
Web of Science ID：WOS:000175895400012 Cold acclimation-induced WAP27 localized in endoplasmic reticulum in cortical parenchyma cells of mulberry tree was homologous to group 3 late-embryogenesis abundant proteins N Ukaji; C Kuwabara; D Takezawa; K Arakawa; S Fujikawa
PLANT PHYSIOLOGY,
Volume：126,
Number：4,
First page：1588,
Last page：1597, Aug. 2001,
[Reviewed]English, Scientific journal
DOI：https://doi.org/10.1104/pp.126.4.1588DOI ID：10.1104/pp.126.4.1588,
ISSN：0032-0889,
CiNii Articles ID：80012556716,
PubMed ID：11500557,
Web of Science ID：WOS:000170413800026 A rapid induction by elicitors of the mRNA encoding CCD-1, a 14 kDa Ca2+-binding protein in wheat cultured cells
D Takezawa
PLANT MOLECULAR BIOLOGY, Volume：42, Number：6, First page：807, Last page：817, Apr. 2000, [Reviewed]
English, Scientific journal
ISSN：0167-4412, Web of Science ID：WOS:000087182000002
Elicitor- and A23187-induced expression of WCK-1, a gene encoding mitogen-activated protein kinase in wheat
D Takezawa
PLANT MOLECULAR BIOLOGY, Volume：40, Number：6, First page：921, Last page：933, Aug. 1999, [Reviewed]
English, Scientific journal
ISSN：0167-4412, Web of Science ID：WOS:000082567400003
Akinete formation in Tribonema bombycinum derbes et solier (Xanthophyceae) in relation to freezing tolerance
M Nagao; K Arakawa; D Takezawa; S Yoshida; S Fujikawa
JOURNAL OF PLANT RESEARCH, Volume：112, Number：1106, First page：163, Last page：174, Jun. 1999, [Reviewed]
English, Scientific journal
ISSN：0918-9440, Web of Science ID：WOS:000087329700003
Alterations of intracellular pH in response to low temperature stresses
S Yoshida; K Hotsubo; Y Kawamura; M Murai; K Arakawa; D Takezawa
JOURNAL OF PLANT RESEARCH, Volume：112, Number：1106, First page：225, Last page：236, Jun. 1999, [Reviewed]
English, Scientific journal
ISSN：0918-9440, Web of Science ID：WOS:000087329700009
Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation
N Ukaji; C Kuwabara; D Takezawa; K Arakawa; S Yoshida; S Fujikawa
PLANT PHYSIOLOGY, Volume：120, Number：2, First page：481, Last page：489, Jun. 1999, [Reviewed]
English, Scientific journal
ISSN：0032-0889, Web of Science ID：WOS:000080936500015
Accumulation of 19-kDa plasma membrane polypeptide during induction of freezing tolerance in wheat suspension-cultured cells by abscisic acid
M Koike; D Takezawa; K Arakawa; S Yoshida
PLANT AND CELL PHYSIOLOGY, Volume：38, Number：6, First page：707, Last page：716, Jun. 1997, [Reviewed]
English, Scientific journal
ISSN：0032-0781, Web of Science ID：WOS:A1997XF27600009
Functional domains of plant chimeric calcium/calmodulin-dependent protein kinase: Regulation by autoinhibitory and visinin-like domains
S Ramachandiran; D Takezawa; W Wang; BW Poovaiah
JOURNAL OF BIOCHEMISTRY, Volume：121, Number：5, First page：984, Last page：990, May 1997, [Reviewed]
English, Scientific journal
ISSN：0021-924X, Web of Science ID：WOS:A1997WY02200028
Characterization of vacuolar H+-ATPases that are sensitive and tolerant to cold
K Hotsubo; Y Kawamura; D Takezawa; K Arakawa; S Yoshida
PLANT COLD HARDINESS, First page：237, Last page：244, 1997, [Reviewed]
English, International conference proceedings
Web of Science ID：WOS:000071892500021
Regulated expression of a calmodulin isoform alters growth and development in potato
BW Poovaiah; D Takezawa; G An; TJ Han
JOURNAL OF PLANT PHYSIOLOGY, Volume：149, Number：5, First page：553, Last page：558, Oct. 1996, [Reviewed]
English, Scientific journal
ISSN：0176-1617, Web of Science ID：WOS:A1996VM27500012
Calcium-dependent protein kinase genes in corn roots
D Takezawa; S Patil; A Bhatia; BW Poovaiah
JOURNAL OF PLANT PHYSIOLOGY, Volume：149, Number：3-4, First page：329, Last page：335, Aug. 1996, [Reviewed]
English, Scientific journal
ISSN：0176-1617, Web of Science ID：WOS:A1996VH80200016
A potato cDNA encoding a homologue of mammalian multidrug resistant P-glycoprotein
W Wang; D Takezawa; BW Poovaiah
PLANT MOLECULAR BIOLOGY, Volume：31, Number：3, First page：683, Last page：687, Jun. 1996, [Reviewed]
English, Scientific journal
ISSN：0167-4412, Web of Science ID：WOS:A1996VE97300021
Dual regulation of a chimeric plant serine threonine kinase by calcium and calcium calmodulin
D Takezawa; S Ramachandiran; Paranjape, V; BW Poovaiah
JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：271, Number：14, First page：8126, Last page：8132, Apr. 1996, [Reviewed]
English, Scientific journal
ISSN：0021-9258, Web of Science ID：WOS:A1996UD60000040
CHIMERIC PLANT CALCIUM/CALMODULIN-DEPENDENT PROTEIN-KINASE GENE WITH A NEURAL VISININ-LIKE CALCIUM-BINDING DOMAIN
S PATIL; D TAKEZAWA; BW POOVAIAH
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：92, Number：11, First page：4897, Last page：4901, May 1995, [Reviewed]
English, Scientific journal
ISSN：0027-8424, Web of Science ID：WOS:A1995RA15000037
The bHLH transcription factor MpHYPNOS regulates gemma dormancy in the liverwort Marchantia polymorpha Hirotaka Kato; Nami Yoshimura; Mikako Yoshikawa; Hideyuki Matsuura; Kosaku Takahashi; Daisuke Takezawa; Tomoyuki Furuya; Yuki Kondo; Hidehiro Fukaki; Tetsuro Mimura; Kimitsune Ishizaki
bioRxiv,
First page：488978, 25 Apr. 2022
DOI：https://doi.org/10.1101/2022.04.25.488978DOI ID：10.1101/2022.04.25.488978 Long- and short-term freezing induce different types of injury in Arabidopsis thaliana leaf cells M. Nagao; K. Arakawa; D. Takezawa; S. Fujikawa
PLANTA,
Volume：227,
Number：2,
First page：477,
Last page：489, Jan. 2008
English
DOI：https://doi.org/10.1007/s00425-007-0633-9DOI ID：10.1007/s00425-007-0633-9,
ISSN：0032-0935,
CiNii Articles ID：80017945202,
PubMed ID：17924137,
Web of Science ID：WOS:000251373400018 Gene expression associated with increased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi) Naoki Takata; Jun Kasuga; Daisuke Takezawa; Keita Arakawa; Seizo Fujikawa
JOURNAL OF EXPERIMENTAL BOTANY,
Volume：58,
Number：13,
First page：3731,
Last page：3742, Oct. 2007
English
DOI：https://doi.org/10.1093/jxb/erm223DOI ID：10.1093/jxb/erm223,
ISSN：0022-0957,
eISSN：1460-2431,
CiNii Articles ID：80019361940,
PubMed ID：18057043,
Web of Science ID：WOS:000251506300020 Gene expression associated with increased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi) Naoki Takata; Jun Kasuga; Daisuke Takezawa; Keita Arakawa; Seizo Fujikawa
Journal of Experimental Botany,
Volume：58,
Number：13,
First page：3731,
Last page：3742, Oct. 2007
English
DOI：https://doi.org/10.1093/jxb/erm223DOI ID：10.1093/jxb/erm223,
ISSN：0022-0957,
CiNii Articles ID：80019361940,
PubMed ID：18057043,
SCOPUS ID：36849057130 Reduced sensitivity to freezing and dehydration in the abscisic acid-insensitive mutant in mosses
Daisuke Takezawa
PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S240, Last page：S240, 2007
English, Summary international conference
ISSN：0032-0781, Web of Science ID：WOS:000245922701436
植物の脱水応答に関わるシグナル因子の探索
竹澤 大輔
Number：5(18年度), First page：653, Last page：654, 2007
植物の脱水応答に関わるシグナル因子の探索
竹澤 大輔
総合研究機構研究プロジェクト研究成果報告書, Number：5(18年度), First page：653, Last page：654, 2007
Accumulation of theanderose in association with development of freezing tolerance in the moss Physcomitrella patens M Nagao; K Oku; A Minami; K Mizuno; M Sakurai; K Arakawa; S Fujikawa; D Takezawa
PHYTOCHEMISTRY,
Volume：67,
Number：7,
First page：702,
Last page：709, Apr. 2006
English
DOI：https://doi.org/10.1016/j.phytochem.2006.01.031DOI ID：10.1016/j.phytochem.2006.01.031,
ISSN：0031-9422,
CiNii Articles ID：80019277810,
PubMed ID：16527318,
Web of Science ID：WOS:000236819800009 Altered Freezing Tolerance in the Physcomitrella patens Mutant with Reduced Sensitivity to Abscisic Acid
MINAMI Anzu; TOGAWA Saki; NAGAO Manabu; TAKEZAWA Daisuke
Cryobiology and cryotechnology, Volume：52, Number：2, First page：135, Last page：139, 2006
Many types of plant cells accumulate low molecular-weight soluble sugars when they acquire freezing tolerance in the process of cold acclimation. Protonema cells of the moss Physcomitrella patens develop freezing tolerance upon treatment with the phytohormone abscisic acid (ABA). We previously reported that the ABA-treated protonema cells accumulate low molecular-weight soluble sugars such as sucrose and theanderose. To clarify mechanisms underlying physiological processes leading to development of freezing tolerance, we isolated F patens mutant lines, AR1 to AR7, with reduced sensitivity to ABA. Among these lines, AR7 showed growth similar to wild type but did not develop freezing tolerance by ABA treatment. Analysis of low molecular-weight soluble sugars indicated that the AR7 cells accumulated sucrose upon ABA treatment but very little of theanderose.
Japanese Society for Cryobiology and Cryotechnology, English
DOI ID：10.20585/cryobolcryotechnol.52.2_135, ISSN：1340-7902, CiNii Articles ID：110006783031, CiNii Books ID：AN10448734
植物の脱水ストレス耐性機構の解明
竹澤 大輔
Number：4(17年度), 2006
Alterered Freezing tolerance in teh Physcomitrella patens mutant with reduced sensitivity to abscisic acid. Minami, A; Togawa, S; Nagao, M; Takezawa D
Cryobioloby and Cryotechnology,
Volume：52,
First page：135,
Last page：139, 2006
DOI：https://doi.org/10.20585/cryobolcryotechnol.52.2_135DOI ID：10.20585/cryobolcryotechnol.52.2_135 Physiological and morphological alteration associated with freezing tolerance in the moss Physcomitrella patens.
Minami, A; Nagao, M; Arakawa, K; Fujikawa, S; Takezawa, D
lant Cold Hardiness, CABI, First page：138, Last page：152, 2006
植物の脱水ストレス耐性機構の解明
竹澤 大輔
総合研究機構研究プロジェクト研究成果報告書, Number：4(17年度), 2006
Rapid degradation of starch in chloroplasts and concomitant accumulation of soluble sugars associated with ABA-induced freezing tolerance in the moss Physcomitrefla patens M Nagao; A Minami; K Arakawa; S Fujikawa; D Takezawa
JOURNAL OF PLANT PHYSIOLOGY,
Volume：162,
Number：2,
First page：169,
Last page：180, Feb. 2005
English
DOI：https://doi.org/10.1016/j.jplph.2004.06.012DOI ID：10.1016/j.jplph.2004.06.012,
ISSN：0176-1617,
Web of Science ID：WOS:000227721100005 Crystal structure of PR-5 protein from winter wheat Kuwabara Chikako; Kondo Hidemasa; Noro Natsuko; Takezawa Daisuke; Arakawa Keita; Tsuda Sakae
Plant and Cell Physiology Supplement,
Volume：2005,
Number：0,
First page：848,
Last page：848, 2005
WAS-3 protein, a member of PR-5 family, accumulates in an apoplastic space of winter wheat in response to cold acclimation. We previously revealed that recombinant WAS-3 protein (rWAS-3) produced using high efficiency wheat expression system inhibited hyphal growth of pink snow mold and
Fusarium oxysporum. Furthermore, rWAS-3 bound to fungal cell wall itself and β-1,3-glucan that was main components of fungal cell walls. In order to clarify the biochemical interaction of WAS-3 and β-1,3-glucan, we determined the crystal structure of rWAS-3 using X-ray crystallographic analysis. The thin plate-shaped crystals of rWAS-3 were obtained by hanging-drop vapor diffusion method. The structure of rWAS-3 was determined at 1.8 angstrom resolution by molecular replacement method. The electrostatic potential analysis revealed that rWAS-3 had an acidic cleft region. Therefore, we concluded that this region might be β-1,3-glucan binding site of rWAS-3.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2005.0.848.0DOI ID：10.14841/jspp.2005.0.848.0,
CiNii Articles ID：130006988465 Reduced tolerance to freezing and osmotic stress in Physcomitrella ABA-insensitive mutants Minami Anzu; Nagao Manabu; Arakawa Keita; Fujikawa Seizo; Takezawa Daisuke
Plant and Cell Physiology Supplement,
Volume：2005,
Number：0,
First page：222,
Last page：222, 2005
Abscisic acid (ABA) is known to play an important role in responses to environmental stresses. We are investigating mechanisms of ABA-induced freezing tolerance in the moss
Physcomitrella patens. ABA induced physiological changes such as expression of various genes, accumulation of boiling soluble proteins and increase in soluble sugar contents.
We isolated mutants with reduced ABA sensitivity by UV mutagenesis. These mutants grew normally in a medium containing ABA, whereas the wild type showed inhibited growth. Freezing and osmotic stress tolerance in these mutants was lower than that of wild type, but the extents of tolerance varied among the mutants. In these mutants, levels of expression of stress-related genes, a few boiling-soluble proteins and soluble sugars after ABA treatment were reduced as compared with those of wild-type. The degrees of freezing tolerance were correlated with levels of proteins and sugars in ABA-insensitive mutants.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2005.0.222.0DOI ID：10.14841/jspp.2005.0.222.0,
CiNii Articles ID：130006988346 Cryoprotective activity of winter- accumulating WAP27 protein from mulberry cortical parenchyma cells Nagao Manabu; Ukaji Norifumi; Takezawa Daisuke; Arakawa Keita; Fujikawa Seizo
Plant and Cell Physiology Supplement,
Volume：2005,
Number：0,
First page：221,
Last page：221, 2005
Freezing tolerance of cortical parenchyma cells of mulberry (
Morus bombycis) drastically increases in winter. 27-kDa group 3 late embryogenesis protein (WAP27) accumulated in ER of the cells in winter.
In natural conditions, plants are subjected to prolonged freezing that at high subzero temperature results in prolonged exposure of cells to concentrated solutions. We therefore examined cryoprotection of recombinant WAP27 (rWAP27) on lactate dehydrogenase (LDH) activity against prolonged freezing. The rWAP27 preserved above 80% of LDH activity during prolonged freezing at -2, -4, -10, -20
oC. The cryoprotective activity of bovine serum albumin was nearly equal to that of rWAP27 during prolonged freezing at -20
oC, but decreased with freezing time at -2 and -4
oC. The cryoprotective activity of ovalbumin was also decreased with freezing time at -2 to -20
oC. The rWAP27 has a high cryoprotective activity to freeze-labile proteins against prolonged freezing.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2005.0.221.0DOI ID：10.14841/jspp.2005.0.221.0,
CiNii Articles ID：130006988344 Cold acclimation in bryophytes: low-temperature-induced freezing tolerance in Physcomitrella patens is associated with increases in expression levels of stress-related genes but not with increase in level of endogenous abscisic acid A Minami; M Nagao; K Ikegami; T Koshiba; K Arakawa; S Fujikawa; D Takezawa
PLANTA,
Volume：220,
Number：3,
First page：414,
Last page：423, Jan. 2005
English
DOI：https://doi.org/10.1007/s00425-004-1361-zDOI ID：10.1007/s00425-004-1361-z,
ISSN：0032-0935,
CiNii Articles ID：80017121813,
PubMed ID：15349781,
Web of Science ID：WOS:000226810700006 Accumulation of pathogenesis-related (PR) 10/Bet v 1 protein homologues in mulberry (Morus bombycis Koidz.) tree during winter N Ukaji; C Kuwabara; D Takezawa; K Arakawa; S Fujikawa
PLANT CELL AND ENVIRONMENT,
Volume：27,
Number：9,
First page：1112,
Last page：1121, Sep. 2004
English
DOI：https://doi.org/10.1111/j.1365-3040.2004.01216.xDOI ID：10.1111/j.1365-3040.2004.01216.x,
ISSN：0140-7791,
eISSN：1365-3040,
Web of Science ID：WOS:000223661900004 Calmodulin-binding proteins in bryophytes: identification of abscisic acid-, cold-, and osmotic stress-induced genes encoding novel membrane-bound transporter-like proteins D Takezawa; A Minami
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,
Volume：317,
Number：2,
First page：428,
Last page：436, Apr. 2004
English
DOI：https://doi.org/10.1016/j.bbrc.2004.03.052DOI ID：10.1016/j.bbrc.2004.03.052,
ISSN：0006-291X,
CiNii Articles ID：80016610378,
PubMed ID：15063776,
Web of Science ID：WOS:000220922200020 Analysis of genes involved in acquisition of freezing tolerance in mosses Minami Anzu; Nagao Manabu; Arakawa Keita; Fujikawa Seizo; Takezawa Daisuke
Plant and Cell Physiology Supplement,
Volume：2004,
Number：0,
First page：803,
Last page：803, 2004
Bryophytes live throughout the world from the tropics to Antarctica. Many mosses can grow in extreme environment such as lands with dry and cold seasons. The moss
Physcomitrella patens acquires high freezing tolerance (FT) upon treatment with abscisic acid (ABA) and survives after cryopreservation. We demonstrated that ABA induced morphological changes in cellular organelles and accumulation of boiling-stable proteins and soluble sugars. The increase in FT was strongly inhibited by cycloheximide suggesting critical roles of synthesis of nuclear encoded protein in ABA-induced FT.
To identify genes involved in development of FT of
P. patens, we carried out differential screening. Results of analysis of more than 60 isolated clones indicated that a large proportion of the ABA-induced genes had similarity to higher plant stress-induced genes such as those encoding LEA and aquaporin, whereas several other genes encoded proteins not conserved in higher plants such as LI818 and UVI-1.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2004.0.803.0DOI ID：10.14841/jspp.2004.0.803.0,
CiNii Articles ID：130006987517 Characterization of a novel plant PP2C-like protein Ser/Thr phosphatase as a calmodulin-binding protein Daisuke Takezawa
Journal of Biological Chemistry,
Volume：278,
Number：39,
First page：38076,
Last page：38083, 26 Sep. 2003
English
DOI：https://doi.org/10.1074/jbc.M301369200DOI ID：10.1074/jbc.M301369200,
ISSN：0021-9258,
CiNii Articles ID：80016236736,
PubMed ID：12860996,
SCOPUS ID：0141620376 Characterization of a novel plant PP2C-like protein Ser/Thr phosphatase as a calmodulin-binding protein D Takezawa
JOURNAL OF BIOLOGICAL CHEMISTRY,
Volume：278,
Number：39,
First page：38076,
Last page：38083, Sep. 2003
English
DOI：https://doi.org/10.1074/jbc.M301369200DOI ID：10.1074/jbc.M301369200,
ISSN：0021-9258,
CiNii Articles ID：80016236736,
PubMed ID：12860996,
Web of Science ID：WOS:000185437200129 Abscisic acid-induced freezing tolerance in the moss Physcomitrella patens is accompanied by increased expression of stress-related genes A Minami; M Nagao; K Arakawa; S Fujikawa; D Takezawa
JOURNAL OF PLANT PHYSIOLOGY,
Volume：160,
Number：5,
First page：475,
Last page：483, May 2003
English
DOI：https://doi.org/10.1078/0176-1617-00888DOI ID：10.1078/0176-1617-00888,
ISSN：0176-1617,
Web of Science ID：WOS:000183064100006 Characterization of Physcomitrella PP2C-like protein phosphatase that binds calmodulin
D Takezawa
PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S40, Last page：S40, 2003
English, Summary international conference
ISSN：0032-0781, Web of Science ID：WOS:000181914300157
PR-10/Bet v 1 protein family localized in cytosol and nucleus Ukaji Norifumi; Takezawa Daisuke; Arakawa Keita; Fujikawa Seizo
Plant and Cell Physiology Supplement,
Volume：2003,
Number：0,
First page：308,
Last page：308, 2003
During seasonal cold acclimation, 18 kD proteins, designated WAP18, were accumulated in the soluble protein fractions in mulberry (
Morus bombycis Koidz.) tree. The purified WAP18 showed cryoprotective activity against freeze-labile lactate-dehydrogenase, LDH, suggesting that WAP18 has roles to contribute freezing tolerance in cortical parenchyma cells of mulberry tree in winter. The cDNA cloning revealed that WAP18 has high sequence similarity to pathogenesis-related (PR)-10/Bet v 1 protein family. Northern blot analysis showed that transcript levels of WAP18 increased not only by cold treatment at 4
oC but also by wounding, ethephon and salicylic acid.
It has been predicted that PR-10/Bet v 1 proteins localize in the cytosol based on the absence of apparent signal sequence. Immuno-electronmicroscpe analysis showed that gold particles against anti-WAP18 antibodies localized not only in cytosol but also in nucleus in mulberry tree. This result indicates that PR-10/Betv1 proteins localize in cytosol and nucleus.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2003.0.308.0DOI ID：10.14841/jspp.2003.0.308.0,
CiNii Articles ID：130006989896 Functional Analysis of ABA-and Cold-Responsive Protein Kinase in Physcomitrella patens Minami Anzu; Nagao Manabu; Arakawa Keita; Fujikawa Seizo; Takezawa Daisuke
Plant and Cell Physiology Supplement,
Volume：2003,
Number：0,
First page：459,
Last page：459, 2003
Protein phosphorylation and dephosphorylation are implicated in the development of freezing tolerance during cold acclimation in plants. We previously showed that freezing tolarance of moss
Physcomitrella patens protonema cells increased by treatment with ABA, cold. We isolated the
PARK gene encoding protein Ser/Thr kinase induced by ABA and cold. The PARK kinase domain was similar to S-domain receptor like protein kinase, but it did not possess the extracellular domain.
The GST-PARK fusion protein phosphorylated histon IIIS and myelin basic protein and that the kinase itself underwent rapid autophosphorylation. When the PARK-GFP fusion gene was introduced into plant cells, the fluorescence signal was observed in the plasma membrane. Gene knock-out plants of
PARK had reduced survival rates after freezing as compared to the wild type plants. These results indicate that PARK might regulate signal transduction processes of ABA- or cold-induced freezing tolerance.
The Japanese Society of Plant Physiologists
DOI：https://doi.org/10.14841/jspp.2003.0.459.0DOI ID：10.14841/jspp.2003.0.459.0,
CiNii Articles ID：130006987934 Molecular chaperone activity of ER-localized small heat shock proteins that accumulated in cortical parenchyma cells of mulberry tree during winter in vitro
N Ukaji; D Takezawa; K Arakawa; S Fujikawa
PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S116, Last page：S116, 2002
English, Summary international conference
ISSN：0032-0781, Web of Science ID：WOS:000174726400420
Analyses of calmodulin-dependent protein kinase in moss Physcomitrella patens
D Takezawa
PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S53, Last page：S53, 2002
English, Summary international conference
ISSN：0032-0781, Web of Science ID：WOS:000174726400188
ABA-induced freezing tolerance in moss Physcomitrella patens and physiological changes
M Nagao; A Minami; K Arakawa; S Fujikawa; D Takezawa
PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S168, Last page：S168, 2002
English, Summary international conference
ISSN：0032-0781, Web of Science ID：WOS:000174726400601
ABA-INDUCED FREEZING TOLERANCE IN Physcomitrella patens AND GENE EXPRESSION :
NAGAO Manabu; MINAMI Anzu; TAKEZAWA Daisuke; ARAKAWA Keita; FUJIKAWA Seizo
Plant and cell physiology, Volume：42, First page：s121, 2001
Japanese Society of Plant Physiologists, English
ISSN：0032-0781, CiNii Articles ID：110003715616, CiNii Books ID：AA0077511X
Accumulation of 18kD Intracellular Pathogenesis-Related Proteins in Cortical Parenchyma Cells of Mulberry Tree during Seasonal Cold Acclimation :
Ukaji Norifumi; Takezawa Daisuke; Arakawa Keita; Fujikawa Seizo
Plant and cell physiology, Volume：42, First page：s143, 2001
Japanese Society of Plant Physiologists, English
ISSN：0032-0781, CiNii Articles ID：110003715703, CiNii Books ID：AA0077511X
The Function of the ABA-induced Thaumatin-like Protein in Winter Wheat Cells :
KUWABARA Chikako; ARAKAWA Keita; TAKEZAWA Daisuke; FUJIKAWA Seizo
Plant and cell physiology, Volume：41, First page：s50, 2000
Japanese Society of Plant Physiologists, English
ISSN：0032-0781, CiNii Articles ID：110003722122, CiNii Books ID：AA0077511X
Gene expression of WAP20 and WAP27 :
UKAJI Norifumi; TAKEZAWA Daisuke; ARAKAWA Keita; FUJIKAWA Seizo
Plant and cell physiology, Volume：41, First page：s48, 2000
Japanese Society of Plant Physiologists, English
ISSN：0032-0781, CiNii Articles ID：110003722116, CiNii Books ID：AA0077511X
DIVERSITY OF PROTEINS EXPRESSED IN OVERWINTERING PLANTS UNDER THE LOW TEMPERATURE ENVIRONMENT :
TAKEZAWA Daisuke; ARAKAWA Keita; YOSHIDA Shizuo; FUJIKAWA Seizo
Plant and cell physiology, Volume：41, First page：s10, 2000
Japanese Society of Plant Physiologists, English
ISSN：0032-0781, CiNii Articles ID：110003721982, CiNii Books ID：AA0077511X
WAS-3, the ABA-induced Thaumatin-like Protein, in Suspension-cultured Cells of Winter Wheat
KUWABARA Chikako; ARAKAWA Keita; TAKEZAWA Daisuke; FUJIKAWA Seizo
Volume：40, First page：s75, Last page：s75, Mar. 1999
English
ISSN：0032-0781, CiNii Articles ID：10003758018, CiNii Books ID：AA0077511X
Molecular Cloning of ABA-induced Secretory Protein (WAS-2) in Winter Wheat Cultured Cells
KUWABARA Chikako; TAKEZAWA Daisuke; ARAKAWA Keita; YOSHIDA Shizuo
Volume：39, First page：S92, Last page：S92, May 1998
English
ISSN：0032-0781, CiNii Articles ID：10003751843, CiNii Books ID：AA0077511X
ABA-INDUCED ACCUMULATION OF A 19-kDa PLASMA MEMBRANE POLYPEPTIDE IN SUSPENSION CULTURED-CELLS OF WINTER WHEAT AND ITS RELATIONSHIP TO THE INDUCTION OF FREEZING TOLERANCE
KOIKE Michiya; TAKEZAWA Daisuke; ARAKAWA Keita; YOSHIDA Shizuo
Volume：38, First page：s97, Mar. 1997
English
ISSN：0032-0781, CiNii Articles ID：10004345773, CiNii Books ID：AA0077511X
Dual regulation of a chimeric plant serine/threonine kinase by calcium/calmodulin
D Takezawa; S Ramachandiran; Paranjape, V; BW Poovaiah
PLANT PHYSIOLOGY, Volume：111, Number：2, First page：155, Last page：155, Jun. 1996
English, Summary international conference
ISSN：0032-0889, Web of Science ID：WOS:A1996UR53400199
