Performance information

• Chloroplast translation factor EF-Tu of Arabidopsis thaliana can be inactivated via oxidation of a specific cysteine residue
  Machi Toriu; Momoka Horie; Yuka Kumaki; Taku Yoneyama; Shin Kore-eda; Susumu Mitsuyama; Keisuke Yoshida; Toru Hisabori; Yoshitaka Nishiyama
  Biochemical Journal, Volume：480, Number：5, First page：307, Last page：318, Feb. 2023, [Reviewed]
  Translational elongation factor EF-Tu, which delivers aminoacyl-tRNA to the ribosome, is susceptible to inactivation by reactive oxygen species (ROS) in the cyanobacterium Synechocystis sp. PCC 6803. However, the sensitivity to ROS of chloroplast-localized EF-Tu (cpEF-Tu) of plants remains to be elucidated. In the present study, we generated a recombinant cpEF-Tu protein of Arabidopsis thaliana and examined its sensitivity to ROS in vitro. In cpEF-Tu that lacked a bound nucleotide, one of the two cysteine residues, Cys149 and Cys451, in the mature protein was sensitive to oxidation by H2O2, with the resultant formation of sulfenic acid. The translational activity of cpEF-Tu, as determined with an in vitro translation system, derived from Escherichia coli, that had been reconstituted without EF-Tu, decreased with the oxidation of a cysteine residue. Replacement of Cys149 with an alanine residue rendered cpEF-Tu insensitive to inactivation by H2O2, indicating that Cys149 might be the target of oxidation. By contrast, cpEF-Tu that had bound either GDP or GTP was less sensitive to oxidation by H2O2 than nucleotide-free cpEF-Tu. Addition of thioredoxin f1, a major thioredoxin in the Arabidopsis chloroplast, to oxidized cpEF-Tu allowed the reduction of Cys149 and the reactivation of cpEF-Tu, suggesting that the oxidation of cpEF-Tu might be a reversible regulatory mechanism that suppresses the chloroplast translation system in a redox-dependent manner.
  Portland Press Ltd., English, Scientific journal
  DOI：https://doi.org/10.1042/bcj20220609
  DOI ID：10.1042/bcj20220609, ISSN：0264-6021, eISSN：1470-8728
• Microbial Community Structures in Terrestrial Subsurface Sediments from the Southern Kanto Plain, Japan　　　　　　　　　　　　　　　
  Satoshi Ohkubo; Takeshi Saito; Muhammad Abul Kalam Azad; Hiromitsu Kawai; Wataru Suda; Shin Kore-eda; Shoichiro Hamamoto; Hirotaka Saito; Takato Takemura; Toshiko Komatsu; Jun-ichi Ohnishi
  GEOMICROBIOLOGY JOURNAL, Volume：37, Number：7, First page：595, Last page：602, Jul. 2020, [Reviewed]
  Microbial community structure reflects the surrounding natural environment and changes to that environment. Although the subsurface at 5-100 m depth is important for human activities and there are potential risks of environmental pollution in this region, there have been only a few reports of subsurface microbial community structures in terrestrial areas. We investigated the diversity and community compositions of Bacteria and Archaea in boring cores collected from various depths at three different sites in the southern Kanto Plain, Japan. The results of 16S rRNA gene amplicon sequencing using MiSeq showed that the microbial community composition varied with the geological unit. Proteobacteria (Alphaproteobacteria and Gammaproteobacteria) were dominant members within sediments accumulated during the Pleistocene in the Musashino Upland. In contrast, Acidobacteria and Chloroflexi characteristically appeared in the Holocene layers of the Arakawa Lowland. These data suggest that the subsurface microbial composition is controlled by the geological features of the sediments.
  TAYLOR & FRANCIS INC, English, Scientific journal
  DOI：https://doi.org/10.1080/01490451.2020.1743390
  DOI ID：10.1080/01490451.2020.1743390, ISSN：0149-0451, eISSN：1521-0529, Web of Science ID：WOS:000523375400001
• Crassulacean acid metabolism induction in Mesembryanthemum crystallinum can be estimated by non-photochemical quenching upon actinic illumination during the dark period.　　　　　　　　　　　　　　　
  Matsuoka T; Onozawa A; Sonoike K; Kore-eda S
  Plant and Cell Physiology, Volume：59, Number：10, First page：1966, Last page：1975, 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcy118
  DOI ID：10.1093/pcp/pcy118
• Arabidopsis glycerol-3-phosphate permease 4 is localized in the plastids and involved in the accumulation of seed oil　　　　　　　　　　　　　　　
  Hiromitsu Kawai; Toshiki Ishikawa; Toshiaki Mitsui; Shin Kore-eda; Maki Kawai-Yamada; Jun-ichi Ohnishi
  PLANT BIOTECHNOLOGY, Volume：31, Number：2, First page：159, Last page：165, 2014, [Reviewed]
  In plant cells, glycerol 3-phosphate (G3P) is apparently able to permeate the plastid envelope, but no specific transporter has been characterized so far. The Arabidopsis five G3Pp proteins have been predicted as putative G3P permeases because of their high homologies with the prokaryotic G3P/phosphate antiporter GlpT. In the present study, G3Pp4 was characterized in detail utilizing reverse genetic approaches. Promoter analysis using GUS expression revealed that G3Pp4 was expressed strongly throughout the embryos during late developmental stages, and the seed lipid contents decreased in two g3pp4 knockout mutants. An enhanced yellow fluorescent protein-fused G3Pp4 was localized in the plastids, functioned physiologically in A. thaliana, and had G3P-transport activity in E. coli. These results suggest that Arabidopsis G3Pp4 is a plastid envelope-localized G3P transporter and involved in accumulation of storage lipids in late embryogenesis.
  JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.14.0222a
  DOI ID：10.5511/plantbiotechnology.14.0222a, ISSN：1342-4580, Web of Science ID：WOS:000339501900009
• Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  Shin Kore-eda; Akira Nozawa; Yusuke Okada; Kazuki Takashi; Muhammad Abul Kalam Azad; Jun-ichi Ohnishi; Yoshitaka Nishiyama; Yuzuru Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：77, Number：7, First page：1511, Last page：1516, Jul. 2013, [Reviewed]
  In plant Mesembryanthemum crystallinum, which has the inducible crassulacean acid metabolism (CAM), isoforms of plastidic phosphate translocators (pPTs) are categorized into three subfamilies: the triose phosphate/phosphate translocator (McTPT1), the phosphoenolpyruvate/phosphate translocator (McPPT1), and the glucose 6-phosphate/phosphate translocator (McGPT1 and McGPT2). In order to elucidate the physiological roles of these pPTs in M. crystallinum, we determined the substrate specificity of each pPT isoform. The substrate specificities of McTPT1, McPPT1, and McGPT1 showed overall similarities to those of orthologs that have been characterized. In contrast, for glucose 6-phosphate, McGPT2 showed higher selectivity than McGPT1 and other GPT orthologs. Because the expression of McGTP2 is specific to CAM while that of McGTP1 is constitutively expressed in both the C-3- and the CAM-state in M. crystallinum, we propose that McGPT2 functions as a CAM system-specific GPT in this plant.
  TAYLOR & FRANCIS LTD, English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.130174
  DOI ID：10.1271/bbb.130174, ISSN：0916-8451, eISSN：1347-6947, Web of Science ID：WOS:000323533800026
• Isolation and characterization of a polyubiquitin gene and its promoter region from mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  Muhammad Abul Kalam Azad; Kunio Morita; Jun-Ichi Ohnishi; Kore-Eda Shin
  Bioscience, Biotechnology and Biochemistry, Volume：77, Number：3, First page：551, Last page：559, 2013, [Reviewed]
  Transcript levels of the polyubiquitin gene McUBI1 had been reported to be constant during Crassulacean acid metabolism (CAM) induction in the facultative CAM plant, Mesembryanthemum crystallinum. Here, we report the sequences of the full-length cDNA of McUBI1 and its promoter, and validation of the McUBI1 promoter as an internal control driving constitutive expression in transient assays using the dual-luciferase system to investigate the regulation of CAM-related gene expression. The McUBI1 promoter drove strong, constitutive expression during CAM induction. We compared the activities of this promoter with those of the cauliflower mosaic virus (CaMV) 35S promoter in detached C3- and CAM-performing M. crystallinum and tobacco leaves. We confirmed stable expression of the genes controlled by the McUBI1 promoter with far less variability than under the CaMV 35S promoter in M. crystallinum, whereas both promoters worked well in tobacco. We found the McUBI1 promoter more suitable than the CaMV 35S promoter as an internal control for transient expression assays in M. crystallinum.
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.120807
  DOI ID：10.1271/bbb.120807, ISSN：0916-8451, PubMed ID：23470760, SCOPUS ID：84876351231
• Transcriptional profiles of organellar metabolite transporters during induction of crassulacean acid metabolism in Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  S Kore-eda; C Noake; M Ohishi; J Ohnishi; JC Cushman
  FUNCTIONAL PLANT BIOLOGY, Volume：32, Number：5, First page：451, Last page：466, 2005, [Reviewed]
  Metabolite transport across multiple organellar compartments is essential for the operation of crassulacean acid metabolism ( CAM). To investigate potential circadian regulation of inter-organellar metabolite transport processes, we have identified eight full-length cDNAs encoding an organellar triose phosphate / P-i translocator (McTPT1), a phosphoenolpyruvate / P-i translocator ( McPPT1), two glucose-6-phosphate /P-i translocators (McGPT1, 2), two plastidic P-i translocator-like proteins (McPTL1, 2), two adenylate transporters (McANT1, 2), a dicarboxylate transporter (McDCT2), and a partial cDNA encoding a second dicarboxylate transporter (McDCT1) in the model CAM plant, Mesembryanthemum crystallinum L. We next investigated day / night changes in steady-state transcript abundance of each of these transporters in plants performing either C-3 photosynthesis or CAM induced by salinity or water-deficit stress. We observed that the expression of both isogenes of the glucose-6-phosphate / P-i translocator ( McGPT1, 2) was enhanced by CAM induction, with McGPT2 transcripts exhibiting much more pronounced diurnal changes in transcript abundance than McGPT1. Transcripts for McTPT1, McPPT1, and McDCT1 also exhibited more pronounced diurnal changes in abundance in the CAM mode relative to the C-3 mode. McGPT2 and McDCT1 transcripts exhibited sustained oscillations for at least 3 d under constant light and temperature conditions suggesting their expression is under circadian clock control. McTPT1 and McGPT2 transcripts were preferentially expressed in leaf tissues in either C-3 or CAM modes. The leaf-specific and / or circadian controlled gene expression patterns are consistent with McTPT1, McGPT2 and McDCT1 playing CAM-specific metabolite transport roles.
  C S I R O PUBLISHING, English, Scientific journal
  DOI：https://doi.org/10.1071/FP04188
  DOI ID：10.1071/FP04188, ISSN：1445-4408, Web of Science ID：WOS:000229373900008
• Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  S Kore-eda; MA Cushman; Akselrod, I; D Bufford; M Fredrickson; E Clark; JC Cushman
  GENE, Volume：341, First page：83, Last page：92, Oct. 2004, [Reviewed]
  The common ice plant, Mesembryanthemum crystallinum, is a halophytic (salt-loving) member of the Aizoaccae, which switches from C-3 photosynthesis to Crassulacean acid metabolism (CAM) when exposed to salinity or water-deficit stress. CAM is a metabolic adaptation of photosynthetic carbon fixation that improves water use efficiency by shifting net CO2 uptake to the night, thereby reducing transpirational water loss. To improve our understanding of the molecular genetic underpinnings and control mechanisms for Crassulacean acid metabolism (CAM) and other salinity stress response adaptations, a total of 9733 expressed sequence tags (ESTs) from cDNAs derived from leaf tissues of well-watered and salinity-stressed (0.5 M NaCl for 30 and 48 h) were characterized. Clustering and assembly of these ESTs resulted in the identification of a total of 3676 tentative unique gene sequences (1249 tentative consensus sequences and 2427 singleton ESTs) expressed in leaves of ice plant under unstressed and salinity stressed conditions. The same number (2782) of ESTs from each library (total=8346 ESTS) were randomly selected and analyzed to compare expression profiles among the control and salt stressed leaf tissues. EST frequencies for transcripts encoding CAM-related enzymes, pathogenesis-related, senescence-associated, cell death-related, and stress-related proteins such as heat shock proteins (HSPs), chaperones, early light-inducible proteins, ion homeostasis, antioxidative stress, detoxification, and biosynthetic enzymes for osmoprotectants increased 2-12-fold in cDNA libraries constructed from salt stressed plants. In contrast, the frequency of ESTs encoding light-harvesting and photosystem complexes and C3 photosynthetic enzymes decreased 4-fold overall following salinity stress with transcripts for ribulose bisphosphate carboxylase/oxygenase (RuBisCO) subunits decreasing 7-fold. Moreover, stressed plants contained a higher percentage of ESTs encoding novel and/or functionally unknown proteins. The rapid discovery of both known and unknown genes related to stress responses in M. crystallinum demonstrates the great utility of EST analysis in unraveling the complex set of adaptive mechanisms contributing to water use efficiency (CAM) and salinity tolerance. (C) 2004 Elsevier B.V. All right reserved.
  ELSEVIER SCIENCE BV, English, Scientific journal
  DOI：https://doi.org/10.1016/j.gene.2004.06.037
  DOI ID：10.1016/j.gene.2004.06.037, ISSN：0378-1119, Web of Science ID：WOS:000224765100008
• Genes that are uniquely stress regulated in salt overly sensitive (sos) mutants　　　　　　　　　　　　　　　
  ZZ Gong; H Koiwa; MA Cushman; A Ray; D Bufford; S Kore-eda; TK Matsumoto; JH Zhu; JC Cushman; RA Bressan; PM Hasegawa
  PLANT PHYSIOLOGY, Volume：126, Number：1, First page：363, Last page：375, May 2001, [Reviewed]
  Repetitive rounds of differential subtraction screening, followed by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mM NaCl for 4 h) genes in Arabidopsis wild-type (Col-0 gl1) seedlings. Probes corresponding to these 84 genes and ACP1, RD22BP1, MYB2, STZ, and PAL were included in an analysis of salt responsive gene expression profiles in gl1 and the salt-hypersensitive mutant sos3. Six of 89 genes were expressed differentially in wild-type and sos3 seedlings; steady-state mRNA abundance of five genes (AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2], AD05B11/S-adenosyl-L-Met:salicylic acid carboxyl methyltransferase [SAMT], AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger [STZ]) was induced and the abundance of one gene (AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt treatment. The expression of CCR1, SAMT, COR6.6/KIN2, and STZ was higher in sos3 than in wild type, and VSP2 and AD06C08/unknown was lower in the mutant. Salt-induced expression of VSP2 in sos1 was similar to wild type, and AD06C08/unknown, CCR1, SAMT, COR6.6/KIN2, and STZ were similar to sos3. VSP2 is regulated presumably by SOS2/3 independent of SOS1, whereas the expression of the others is SOS1 dependent. AD06C08/unknown and VSP2 are postulated to be effecters of salt tolerance whereas CCR1, SAMT, COR6.6/KIN2, and STZ are determinants that must be negatively regulated during salt adaptation. The pivotal function of the SOS signal pathway to mediate ion homeostasis and salt tolerance implicates AD06C08/unknown, VSP2, SAMT, 6.6/KIN2, STZ, and CCR1 as determinates that are involved in salt adaptation.
  AMER SOC PLANT PHYSIOLOGISTS, English, Scientific journal
  DOI：https://doi.org/10.1104/pp.126.1.363
  DOI ID：10.1104/pp.126.1.363, ISSN：0032-0889, Web of Science ID：WOS:000168692300042
• A genomics approach towards salt stress tolerance　　　　　　　　　　　　　　　
  HJ Bohnert; P Ayoubi; C Borchert; RA Bressan; RL Burnap; JC Cushman; MA Cushman; M Deyholos; R Fischer; DW Galbraith; PM Hasegawa; M Jenks; S Kawasaki; H Koiwa; S Kore-eda; BH Lee; CB Michalowski; E Misawa; M Nomura; N Ozturk; B Postier; R Prade; CP Song; Y Tanaka; H Wang; JK Zhu
  PLANT PHYSIOLOGY AND BIOCHEMISTRY, Volume：39, Number：3-4, First page：295, Last page：311, Mar. 2001, [Reviewed]
  Abiotic stresses reduce plant productivity. We focus on gene expression analysis following exposure of plants to high salinity, using salt-shock experiments to mimic stresses that affect hydration and ion homeostasis. The approach includes parallel molecular and genetic experimentation. Comparative analysis is employed to identify functional isoforms and genetic orthologs of stress-regulated genes common to cyanobacteria, fungi, algae and higher plants. We analyze global gene expression profiles monitored under salt stress conditions through abundance profiles in several species: in the cyanobacterium Synechocystis PCC6803, in unicellular (Saccharomyces cerevisiae) and multicellular (Aspergillus nidulans) fungi, the eukaryotic alga Dunaliella salina, the halophytic land plant Mesembryanthemum crystallinum, the glycophytic Oryza sativa and the genetic model Arabidopsis thaliana. Expanding the gene count, stress brings about a significant increase of transcripts for which no function is known. Also, we generate insertional mutants that affect stress tolerance in several organisms. More than 400 000 T-DNA tagged lines of A. thaliana have been generated, and lines with altered salt stress responses have been obtained. Integration of these approaches defines stress phenotypes, catalogs of transcripts and a global representation of gene expression induced by salt stress. Determining evolutionary relationships among these genes, mutants and transcription profiles will provide categories and gene clusters, which reveal ubiquitous cellular aspects of salinity tolerance and unique solutions in multicellular species. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
  ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, English, Scientific journal
  DOI：https://doi.org/10.1016/S0981-9428(00)01237-7
  DOI ID：10.1016/S0981-9428(00)01237-7, ISSN：0981-9428, Web of Science ID：WOS:000168295300011
• Induction of glucose 6-phosphate transport activity in chloroplasts of Mesembryanthemum crystallinum by the C-3-CAM transition　　　　　　　　　　　　　　　
  S Kore-eda; R Kanai
  PLANT AND CELL PHYSIOLOGY, Volume：38, Number：8, First page：895, Last page：901, Aug. 1997, [Reviewed]
  To study possible changes in the transport metabolites between chloroplasts and cytoplasm during CAM induction of Mesembryanthemum crystallinum, we compared substrate specificity of P-i translocator(s) in isolated chloroplasts from the C-3 and CAM-induced plants. The [C-14]glucose 6-phosphate (G6P) transport activity was significant only in the chloroplasts of CAM-mode plants and not detectable in those of C-3-mode, while a similar high rate of [P-32]P-i uptake was observed with both types of chloroplasts. Kinetic analysis of G6P uptake in the CAM chloroplasts showed a high V-max [10.6 mu mol (mg Chl)(-1) h(-1)] and a comparatively low K-m value (0.41 mM); the latter was similar to K-i values of P-i, 3-phosphoglycerate and phosphoenolpyruvate, 0.30, 0.34 and 0.47 mM; respectively. On the other hand, [P-32]P-i uptake in the CAM chloroplasts was inhibited competitively by G6P with a K-i value (8.4 mM) 20-fold higher than the K-m value for G6P uptake, while that in C-3 chloroplasts was not inhibited at all. These results suggest that a new G6P/P-i counterexchange mechanism is induced in the chloroplast envelope of CAM-induced M. crystallinum in addition to the ordinary type of P-i translocator, that cannot transport G6P, already present in the C-3-type chloroplasts.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/oxfordjournals.pcp.a029249
  DOI ID：10.1093/oxfordjournals.pcp.a029249, ISSN：0032-0781, Web of Science ID：WOS:A1997XR15800002
• Induction of light dependent pyruvate transport into chloroplasts of Mesembryanthemum crystallinum by salt stress　　　　　　　　　　　　　　　
  S Kore-eda; T Yamashita; R Kanai
  PLANT AND CELL PHYSIOLOGY, Volume：37, Number：3, First page：257, Last page：262, Apr. 1996, [Reviewed]
  Mode of photosynthesis in Mesembryanthemum crystallinum changes from C-3 to Crassulacean acid metabolism (CAM) when the plants were stressed with high salinity. [C-14]Pyruvate uptake for 30 s into intact chloroplasts isolated from leaves of the CAM mode of M. crystallinum was enhanced more than 5-fold in the light compared with that in the dark. The stromal concentration of pyruvate in the light reached to more than 2.5 times of the medium. In contrast, little or no pyruvate uptake occurred in chloroplasts from C-3 leaves in either light or dark condition, The initial uptake rate (10 s incubation at 4 degrees C) into the CAM chloroplasts in the light was about 3-fold higher than the rate in the dark. K-m and V-max of the initial uptake in the light were 0.54 mM and 8.5 mu mol (mg Chl)(-1) h(-1), respectively. These suggest that pyruvate was actively incorporated into the CAM chloroplasts against its concentration gradient across the envelope in the light, When hydroponically grown M. crystallinum were stressed by 350 mM NaCl, the capacity of chloroplasts for pyruvate uptake was induced in 6 d corresponding to the induction of the activities of PEP-carboxylase and NAD(P)(+)-malic enzymes in response to salt stress.
  OXFORD UNIV PRESS, English, Scientific journal
  DOI：https://doi.org/10.1093/oxfordjournals.pcp.a028940
  DOI ID：10.1093/oxfordjournals.pcp.a028940, ISSN：0032-0781, Web of Science ID：WOS:A1996UJ28900003
• ISOLATION AND CHARACTERIZATION OF THE ADENYLATE-CYCLASE STRUCTURAL GENE OF NEUROSPORA-CRASSA　　　　　　　　　　　　　　　
  S Kore-eda; T Murayama; I Uno
  JAPANESE JOURNAL OF GENETICS, Volume：66, Number：3, First page：317, Last page：334, Jun. 1991, [Reviewed]
  A single gene (nac) encoding an adenylate cyclase was cloned from the genomic DNA library of Neurospora crassa, using the DNA fragment encoding the catalytic domain of adenylate cyclase of Saccharomyces cerevisiae as a probe. The open reading frame of this gene (6900 base pairs) was interrupted three time by introns. The protein encoded consists of 2300 amino acids and has adenylate cyclase activity. N. crassa adenylate cyclase has a high degree of homology with the catalytic domains of yeast and bovine brain adenylate cyclases.
  GENETICS SOC JAPAN, English, Scientific journal
  DOI：https://doi.org/10.1266/jjg.66.317
  DOI ID：10.1266/jjg.66.317, ISSN：0021-504X, Web of Science ID：WOS:A1991GA11600010
• SUPPRESSION OF THE CR-1 MUTATION IN NEUROSPORA-CRASSA　　　　　　　　　　　　　　　
  S Kore-eda; T Murayama; I Uno
  JAPANESE JOURNAL OF GENETICS, Volume：66, Number：1, First page：77, Last page：83, Feb. 1991, [Reviewed]
  We have cloned a DNA fragment, which hybridized with the adenylate cyclase gene (CYR1) of Saccharomyces cerevisiae, from genomic DNA libraries of Neurospora crassa. The cr-1 mutation was able to be suppressed by introducing this DNA fragment on a cosmid vector, judging from recovery of the adenylate cyclase activity and the abnormal morphology.
  GENETICS SOC JAPAN, English, Scientific journal
  DOI：https://doi.org/10.1266/jjg.66.77
  DOI ID：10.1266/jjg.66.77, ISSN：0021-504X, Web of Science ID：WOS:A1991FF42100008

• Subfamilies of mitochondrial pyruvate carrier genes were identified in Poaceae species through deep curation of the genomic sequences　　　　　　　　　　　　　　　
  Kore-eda, Shin; Mitsuyama, Susumu
  IIBMP2020, Sep. 2020, [Lead]
  English, Summary national conference
• 関東南部地下帯水層土壌に含まれる真正細菌叢の網羅的解析　　　　　　　　　　　　　　　
  AZAD Muhammad Abul Kalam; AZAD Muhammad Abul Kalam; 是枝晋; 是枝晋; 斎藤広隆; 斎藤広隆; 竹村貴人; 竹村貴人; 濱本昌一郎; 濱本昌一郎; 大西純一; 大西純一; 大西純一
  Volume：10th, 2013
  J-Global ID：201402248762287017
• 戸田オリンピックボートコースの水質浄化を目指して　　　　　　　　　　　　　　　
  藤原隆司; 是枝晋
  MaLS Forum, Volume：7, Number：7, First page：16, Last page：17, Jan. 2010
  Japanese
  J-Global ID：201002232844173000, CiNii Articles ID：120002081331
• 戸田オリンピックボートコースの水質浄化を目指して　　　　　　　　　　　　　　　
  円谷陽一; 藤原隆司; 是枝晋; 三田和義; 大西純一; 永澤明
  埼玉大学地域オープンイノベーションセンター紀要, Volume：1, Number：1, First page：139, Last page：140, Jun. 2009
  This article describes the attempt to control water pollution in the Toda Olympic boat course using bivalves (Hyriopsis schlegelii). The inspection of the water was carried out and the ability of Hyriopsis schlegelii to filter plankton in the boat course is investigated.
  Japanese
  ISSN：1883-8278, J-Global ID：200902255014487000, CiNii Articles ID：120001491446
• 通性CAM植物アイスプラントの水ストレス応答に必要な転写調節領域の決定　　　　　　　　　　　　　　　
  是枝晋
  Volume：6 (平成19年度), First page：238, Last page：239, 2008
• Structure of glucose-6-Phosphate/Phosphate translocator genes from a facultative CAM plant, Mesembryanthemum crystallinum.　　　　　　　　　　　　　　　
  Shin Kore-eda; Kunio Morita; Kousuke Nakamata; Jun-ichi Ohnishi
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S175, Last page：S175, 2007
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922701178
• 通性ＣＡＭ植物アイスプラントの乾燥耐性獲得に伴う葉緑体輸送体の発現制御　　　　　　　　　　　　　　　
  是枝晋
  Volume：5 (平成18年度), First page：695, Last page：696, 2007
• 通性ＣＡＭ植物アイスプラントの乾燥耐性獲得に伴う葉緑体機能の転換の分子機構　　　　　　　　　　　　　　　
  是枝晋
  Volume：4 (平成17年度), 2006
• Transport characteristics of phosphate translocator proteins in Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  C Noake; S Kore-eda; J Ohnishi
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S183, Last page：S183, 2005
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101234
• Characterization of a membrane protein sharing homology with mammalian Na+/bile acid transporter in ice plant　　　　　　　　　　　　　　　
  M Ohishi; S Kore-eda; J Ohnishi
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S183, Last page：S183, 2005
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101235
• CAM植物・プラスチド型リン酸輸送体遺伝子の発見調節機構　　　　　　　　　　　　　　　
  是枝晋
  Volume：3(平成16年度), 2005
• Differential expression of chloroplastic phosphate translocators in the facultative CAM plant, Mesembryanthemum crystallinum
  是枝晋
  旭硝子財団助成研究成果報告(Web), Volume：2004, First page：04A-C16-P077 (WEB ONLY), 20 Dec. 2004
  Japanese
  J-Global ID：200902200059753000
• Characterization of a cDNA encoding a homologue of mammalian Na+/bile acid transporter from Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  M Ohishi; S Kore-eda
  PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S181, Last page：S181, 2003
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000181914300718
• Tissue specific expression of glucose 6-phosphate/phosphate translocator Isozymes in a facultative CAM plant, Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  S Kore-eda; C Noake; JC Cushman
  PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S64, Last page：S64, 2003
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000181914300253
• ゲノムサイエンスと遺伝子研究　　　　　　　　　　　　　　　
  朝井計; 日原由香子; 太田にじ; 是枝晋; 定家義人
  生物科学, Volume：54, Number：4, First page：229, Last page：237, 2003
  Japanese
  ISSN：0045-2033, CiNii Articles ID：40005787337, CiNii Books ID：AN00129365
• Transcriptional regulation of plastidic phosphate translocators in Mesembryanthemum crystallinum during CAM induction　　　　　　　　　　　　　　　
  S Kore-eda; JC Cushman
  PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S69, Last page：S69, 2002
  OXFORD UNIV PRESS, English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000174726400248
• INHIBITION OF GLUCOSE 6-PHOSPHATE TRANSPORT INTO CHLOROPLASTS FROM THE CAM-MODE OF M. crystallinum BY PYRIDOXAL 5'-PHOSPHATE　　　　　　　　　　　　　　　
  KORE-EDA Shin; KANAI Ryuzi
  Volume：40, First page：s117, Last page：s117, Mar. 1999
  English
  ISSN：0032-0781, CiNii Articles ID：10003758194, CiNii Books ID：AA0077511X
• Na^+-INDUCED ENHANCEMENT OF PYRUVATE TRANSPORT INTO THE CHLOROPLASTS ISOLATED FROM THE CAM MODE OF Mesembryanthemum crystallinum.　　　　　　　　　　　　　　　
  KORE-EDA Shin; YAMASHITA Takashi; KANAI Ryuzi
  Volume：37, First page：39, Last page：39, Mar. 1996
  English
  ISSN：0032-0781, CiNii Articles ID：10002707935, CiNii Books ID：AA0077511X
• COMPARISON OF PYRUVATE UPTAKE INTOMESOPHYLL CHLOROPLAST BETWEEN C_3 - AND CAM-FORM OFMESEMBRYANTHEMUM CRYSTALLINUM　　　　　　　　　　　　　　　
  YAMASHITA Takashi; KORE-EDA Shin; KANAI Ryuzi
  Volume：36, First page：S135, Mar. 1995
  English
  ISSN：0032-0781, CiNii Articles ID：10004344422, CiNii Books ID：AA0077511X

• 植物学の百科事典　　　　　　　　　　　　　　　
  日本植物学会, [Contributor]
  Jun. 2016
• 光合成事典（Web版）　　　　　　　　　　　　　　　
  光合成学会, [Contributor]
  2015
• 光合成事典　　　　　　　　　　　　　　　
  日本光合成研究会, [Contributor]
  Nov. 2003

• Phylogenetic analysis of mitochondrial pyruvate carrier homologues in Panicum miliaceum　　　　　　　　　　　　　　　
  Shin Kore-eda
  The 61st annual meeting of JSPP, Mar. 2020, [Domestic conference]
  Japanese, Poster presentation
• 夜間の励起光照射によりCAM植物葉で高い非光化学消光が誘導されるしくみ　　　　　　　　　　　　　　　
  安藤友樹; 氏家晃弥; 是枝晋
  日本植物生理学会第56回年会, Mar. 2015
  Japanese, Oral presentation, 東京農業大学
• CAM植物に特徴的な夜間の高い非光化学消光　　　　　　　　　　　　　　　
  森谷瑞季; 安藤友樹; 是枝晋
  日本植物学会第78回大会, Sep. 2014
  Japanese, Oral presentation, 明治大学
• アイスプラントのCAM化過程のクロロフィル蛍光測定による解析　　　　　　　　　　　　　　　
  松岡達也; 是枝晋; 園池公毅
  日本植物生理学会第55回年会, Mar. 2014
  Japanese, Oral presentation, 富山大学
• アイスプラントのCAM化で促進されるプロモーター活性の日周制御　　　　　　　　　　　　　　　
  AZAD Muhammad; Abul Kalam; 永田健斗; 大西純一; 是枝晋
  日本植物生理学会第55回年会, Mar. 2014
  Japanese, Poster presentation, 富山大学
• 通性CAM植物アイスプラントのCAM化における下位葉からの転流の役割　　　　　　　　　　　　　　　
  平塚岳; 永田健斗; 大西純一; 是枝晋
  日本植物学会第77回大会, Sep. 2013
  Japanese, Oral presentation, 北海道大学
• シロイヌナズナ種子で貯蔵脂質の蓄積に関与するG3Pp4はプラスチド局在型グリセロール3‐リン酸輸送体である　　　　　　　　　　　　　　　
  河合博光; 石川寿樹; 是枝晋; 川合真紀; 大西純一
  日本植物学会第77回大会, Sep. 2013
  Japanese, Oral presentation, 北海道大学
• シロイヌナズナのグリセロール3‐リン酸輸送体候補タンパクG3Pp4は種子貯蔵脂質の蓄積に関与する　　　　　　　　　　　　　　　
  河合博光; 石川寿樹; 是枝晋; 川合真紀; 大西純一
  日本植物生理学会第54回年会, Mar. 2013
  Japanese, Poster presentation, 岩手大学
• 植物オルガネラ膜輸送体蛋白質の完全インビトロ合成・再構成系による機能解析　　　　　　　　　　　　　　　
  野澤彰; 是枝晋; 隆一輝; 岡田有右; 藤本竜治; 坪井敬文; 戸澤譲
  日本農芸化学会2012年大会, Mar. 2012
  Japanese, Oral presentation, ウェスティン都ホテル京都
• 通性CAM植物アイスプラント・プラスチド型リン酸輸送体の基質特異性　　　　　　　　　　　　　　　
  是枝晋; 野澤彰; 岡田有右; 隆一輝; 西山佳孝; 大西純一; 戸澤譲
  日本植物生理学会第53回年会, Mar. 2012
  Japanese, Oral presentation, 京都産業大学
• The reverse genetic analysis of Arabidopsis G3Pp4, a putative glycerol-3-phosphate (G3P) permease　　　　　　　　　　　　　　　
  Hiromitsu Kawai; Toshiki Ishikawa; Teruaki Sakuma; Maki Kawai; Yasuko Kaneko; Shin Kore-eda; Jun-ichi Ohnishi
  日本植物生理学会第53回年会, Mar. 2012
  Japanese, Poster presentation, 京都産業大学
• 生育と環境に依存したアイスプラントのCAM化　　　　　　　　　　　　　　　
  北原英明; AZAD Muhammad; Abul Kalam; 平塚岳; 大西純一; 是枝晋
  日本植物学会第75回大会, Sep. 2011
  Japanese, Poster presentation, 東京大学
• シロイヌナズナのグリセロール‐3リン酸輸送体ホモログ遺伝子破壊株の表現型の解析　　　　　　　　　　　　　　　
  河合博光; 遠藤雄治; 佐久間輝明; 是枝晋; 大西純一
  日本植物学会第75回大会, Sep. 2011
  Japanese, Poster presentation, 東京大学
• アイスプラントのCAM化で誘導されるプロモーターの植物体の生育と環境に依存した活性化　　　　　　　　　　　　　　　
  ABUL KALAM AZAD Muhammad; 北原英明; 森田邦男; 大西純一; 是枝晋
  日本植物生理学会第52回年会（震災のため中止）, Mar. 2011
  Japanese, Oral presentation, 東北大学
• アイスプラントのCAM化に伴い誘導されるプロモーター領域の機能解析　　　　　　　　　　　　　　　
  AZAD Muhammad; Abul Kalam; 北原英明; 森田邦男; 大西純一; 是枝晋
  日本植物学会第74回大会, Sep. 2010
  Japanese, Oral presentation, 中部大学
• シロイヌナズナのグリセロール‐3‐リン酸輸送体ホモログ破壊株の解析　　　　　　　　　　　　　　　
  遠藤雄治; 佐久間輝明; 大西純一; 是枝晋
  日本植物生理学会第51回年会, Mar. 2010
  Japanese, Poster presentation, 熊本大学
• 通性CAM植物アイスプラント・プラスチド型リン酸輸送体遺伝子ファミリーのプロモーター単離と比較　　　　　　　　　　　　　　　
  AZAD Muhammad; Abul Kalam; 森田邦男; 北原英明; 大西純一; 是枝晋
  日本植物学会第73回大会, Sep. 2009
  Japanese, Oral presentation, 山形大学
• 通性CAM植物アイスプラントの葉組織を使った一過的遺伝子発現系　　　　　　　　　　　　　　　
  森田邦男; 是枝晋; AZAD Muhammad; Abul Kalam; 北原英明; 大西純一
  日本植物学会第72回大会, Sep. 2008
  Japanese, Oral presentation, 高知大学
• アラビドプシス・グリセロール‐3‐リン酸輸送体ホモログ破壊株の性質　　　　　　　　　　　　　　　
  佐久間輝明; 是枝晋; 大西純一
  日本植物生理学会第49回年会, Mar. 2008
  Japanese, Poster presentation, 札幌コンベンションセンター
• 通性CAM植物アイスプラント・グルコース‐6‐リン酸/リン酸輸送体遺伝子の構造　　　　　　　　　　　　　　　
  是枝晋; 森田邦男; 中俣浩介; 大西純一
  日本植物生理学会第48回年会, Mar. 2007
  Japanese, Poster presentation, 愛媛大学
• 通性CAM植物アイスプラントにおけるリン酸輸送体の輸送活性　　　　　　　　　　　　　　　
  野明千雪; 是枝晋; 大西純一
  日本植物生理学会第46回年会, Mar. 2005
  Japanese, Poster presentation, 新潟コンベンションセンター
• ほ乳類Na⁺/胆汁酸輸送体と相同性を持つアイスプラント膜タンパク質の解析　　　　　　　　　　　　　　　
  大石真久; 是枝晋; 大西純一
  日本植物生理学会第46回年会, Mar. 2005
  Japanese, Poster presentation, 新潟コンベンションセンター
• Transcriptional regulation of plastidic metabolite transporters during CAM induction in Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  koreeda shin
  IVth International Congress of Crassulacean Acid Metabolism, Aug. 2004, [International conference]
  English, Poster presentation
• 通性CAM植物アイスプラントにおけるグルコース‐6‐リン酸/リン酸輸送体アイソザイムの発現の組織特異性　　　　　　　　　　　　　　　
  野明千雪; 是枝晋; CUSHMAN J C
  日本植物生理学会第43回年会, Mar. 2003
  Japanese, Oral presentation, 近畿大学
• アイスプラントのCAM化に伴うプラスチドリン酸輸送体の転写産物量の変化　　　　　　　　　　　　　　　
  是枝晋; CUSHMAN J C
  日本植物生理学会第42回年会, Mar. 2002
  Japanese, Oral presentation, 岡山大学
• EST塩基配列情報を利用した通性CAM植物アイスプラントにおける塩ストレス応答の解析　　　　　　　　　　　　　　　
  是枝晋; CUSHMAN M A; 東江栄; CLARK E; CUSHMAN J C
  日本植物生理学会第41回年会, Mar. 2001
  Japanese, Oral presentation, 九州産業大学
• 通性CAM植物・アイスプラントの葉で塩ストレスにより誘導される転写産物のEST解析　　　　　　　　　　　　　　　
  是枝晋; CUSHMAN M A; 東江栄; BUFFORD D; FREDRICKSON M; RAY A; AKSELROD I; LANDRITH D; CUSHMAN J C
  日本植物生理学会第40回年会, Mar. 2000
  Japanese, Oral presentation, 椙山女学園大学
• ピリドキサール5′‐リン酸によるCAM型アイスプラント葉緑体のG6P輸送活性の阻害　　　　　　　　　　　　　　　
  是枝晋; 金井龍二
  日本植物生理学会第39回年会, Mar. 1999
  Japanese, Oral presentation, 東北大学
• Property of G6P transportation activity induced in chloroplast with CAM of ice plant.　　　　　　　　　　　　　　　
  是枝晋; 金井龍二
  日本植物生理学会第37回年会, Mar. 1997
  Japanese, Oral presentation, 京都大学
• Effect of Na+ on pyruvinic acid transport of CAM type ice plant chloroplast.　　　　　　　　　　　　　　　
  是枝晋; 山下卓; 金井龍二
  日本植物生理学会第36回年会, Mar. 1996
  Japanese, Oral presentation, 鹿児島大学
• 通性CAM植物Mesembryanthemum crystalinumのC₃型とCAM誘導植物における葉肉細胞葉緑体ピルビン酸取り込み活性の比較　　　　　　　　　　　　　　　
  山下卓; 是枝晋; 金井龍二
  日本植物生理学会第35回年会, Mar. 1995
  Japanese, Oral presentation, 島根大学

• JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY
• THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH
• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS
• THE BOTANICAL SOCIETY OF JAPAN

• 通性CAM植物アイスプラントの葉緑体リン酸輸送体の発現調節　　　　　　　　　　　　　　　
  Apr. 2002 - Mar. 2004
  Principal investigator
  Grant amount(Total)：2000000
  Competitive research funding
• 葉緑体包膜における代謝産物の輸送機構の解明　　　　　　　　　　　　　　　
  Apr. 1998 - Mar. 1999
  Principal investigator
  Grant amount(Total)：2946000
  Competitive research funding
• Change in the pathway of carbon-fixation from C_3 to C_4 by some amphibious Cyperacean plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for General Scientific Research (C), 1993 - 1994
  KANAI Ryuzi; KORE-EDA Shin, Saitama University
  Grant amount(Total)：2100000, Direct funding：2100000
  An Cyperacean plant, Eleocharis vivipara, was reported by Ueno and coworkers (1988) to have C_4 and C_3 mode of photosynthetic carbon pathway in its terrestrial (T) and submersed (S) form of growth, respectively. For further characterization of this and other ambphibious Eleocharis species, we developed in vitro culture system to remove contaminating algae attached to S from of plants in nature and to control the environmental conditions precisely.
  Transferring E.vivipara from T to S from, enzyme activities of C_4 pathway, PEP carboxylase and NAD-malic enzyme, decreased remarkably but those of Calvin cycle, RuBP carboxylase and NADP-GA3P dehydrogenase increased in some extent. The former activities also decreased when T form was grown in the air containing 1% CO_2. In E.retroflexa, however, enzymes of C_4 pathway kept their high activity in both T and S froms. Enzyme activities of photorespiratory glycolate pathway, such as glycolate oxidase, catalase, serine hydroxymethyltransferase and hydroxypyruvate reductase, were very low in T form of both Eleocharis species, and no increase was obseved by transferring to S from. Thus, S form of E.vivipara seems to be a C_3 plant having low photorespiratory activity, which is similar to some of submersed macrophyte (SAM) species ; while E.retroflexa retains capacity of C_4 pathway even in its S form. Carbonic anhydrase activity did not change much by transfer from T to S from in both species.
  Grant number：05640724
• CAM植物の組織培養を用いた酸代謝誘導系の確立　　　　　　　　　　　　　　　
  Apr. 1992 - Mar. 1993
  Principal investigator
  Grant amount(Total)：900000
  Competitive research funding

• 戸田ボートコース水質調査　　　　　　　　　　　　　　　
  Academic research planning
  Apr. 2009 - Present
  Academic research
• 「戸田オリンピックボートコースの水質浄化を目指して」に伴う水質調査　　　　　　　　　　　　　　　
  Academic research planning
  Oct. 2008 - Mar. 2009
  Academic research