Performance information

• The deficiency of methylglyoxal synthase promotes cell proliferation in Synechocystis sp. PCC 6803 under mixotrophic conditions　　　　　　　　　　　　　　　
  Aikeranmu Kadeer; Yuuma Ishikawa; Kaushalya Dayarathne; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Plant Biotechnology, Volume：41, Number：4, First page：393, Last page：399, Dec. 2024, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.24.0718a
  DOI ID：10.5511/plantbiotechnology.24.0718a
• Production of extracellular superoxide contributes to photosynthesis via elimination of reducing power and regeneration of NADP+ in the red-tide-forming raphidophyte Chattonella marina complex　　　　　　　　　　　　　　　
  Koki Yuasa; Takayoshi Ichikawa; Yuma Ishikawa; Haruhiko Jimbo; Maki Kawai-Yamada; Tomoyuki Shikata; Yoshitaka Nishiyama
  Harmful Algae, Volume：139, First page：102712, Last page：102712, Nov. 2024, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.hal.2024.102712
  DOI ID：10.1016/j.hal.2024.102712, ISSN：1568-9883
• Arabidopsis DREB26/ERF12 and its close relatives regulate cuticular wax biosynthesis under drought stress condition
  Kaoru Urano; Yoshimi Oshima; Toshiki Ishikawa; Takuma Kajino; Shingo Sakamoto; Mayuko Sato; Kiminori Toyooka; Miki Fujita; Maki Kawai‐Yamada; Teruaki Taji; Kyonoshin Maruyama; Kazuko Yamaguchi‐Shinozaki; Kazuo Shinozaki
  The Plant Journal, Volume：120, Number：5, First page：2057, Last page：2075, Oct. 2024, [Reviewed]
  SUMMARY
  
  Land plants have evolved a hydrophobic cuticle on the surface of aerial organs as an adaptation to ensure survival in terrestrial environments. Cuticle is mainly composed of lipids, namely cutin and intracuticular wax, with epicuticular wax deposited on plant surface. The composition and permeability of cuticle have a large influence on its ability to protect plants against drought stress. However, the regulatory mechanisms underlying cuticular wax biosynthesis in response to drought stress have not been fully elucidated. Here, we identified three AP2/ERF transcription factors (DREB26/ERF12, ERF13 and ERF14) involved in the regulation of water permeability of the plant surface. Transmission electron microscopy revealed thicker cuticle on the leaves of DREB26‐overexpressing (DREB26OX) plants, and thinner cuticle on the leaves of transgenic plants expressing SRDX repression domain‐fused DREB26 (DREB26SR). Genes involved in cuticular wax formation were upregulated in DREB26OX and downregulated in DREB26SR. The levels of very‐long chain (VLC) alkanes, which are a major wax component, increased in DREB26OX leaves and decreased in DREB26SR leaves. Under dehydration stress, water loss was reduced in DREB26OX and increased in DREB26SR. The erf12/13/14 triple mutant showed delayed growth, decreased leaf water content, and reduced drought‐inducible VLC alkane accumulation. Taken together, our results indicate that the DREB26/ERF12 and its closed family members, ERF13 and ERF14, play an important role in cuticular wax biosynthesis in response to drought stress. The complex transcriptional cascade involved in the regulation of cuticular wax biosynthesis under drought stress conditions is discussed.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1111/tpj.17100
  DOI ID：10.1111/tpj.17100, ISSN：0960-7412, eISSN：1365-313X
• The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia.　　　　　　　　　　　　　　　
  Kaushalya Dayarathne; Toshiki Ishikawa; Aikeranmu Kadeer; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Archives of microbiology, Volume：206, Number：9, First page：367, Last page：367, Aug. 2024, [Reviewed], ［International magazine］
  2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00203-024-04099-w
  DOI ID：10.1007/s00203-024-04099-w, PubMed ID：39105810
• Metabolic diversity analysis and genome wide assessment of oxalate accumulation in the leaves of rice Oryza sativa cultivars
  Atsuko Miyagi; Nobuhiro Tanaka; Matthew Shenton; Kaworu Ebana; Satoshi Ohkubo; Shunsuke Adachi; Taiichiro Ookawa; Maki Kawai-Yamada
  Plant Biotechnology, Volume：41, First page：1, Last page：7, Mar. 2024, [Reviewed]
  Japanese Society for Plant Cell and Molecular Biology, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.23.1025a
  DOI ID：10.5511/plantbiotechnology.23.1025a, ISSN：1342-4580, eISSN：1347-6114
• Adjustment of light-responsive NADP dynamics in chloroplasts by stromal pH
  Yusuke Fukuda; Chinami Ishiyama; Maki Kawai-Yamada; Shin-nosuke Hashida
  Nature Communications, Volume：14, Number：1, Nov. 2023, [Reviewed]
  Abstract
  
  Cyclic electron transfer (CET) predominates when NADP⁺ is at basal levels, early in photosynthetic induction; however, the mechanism underlying the subsequent supply of NADP⁺ to fully drive steady-state linear electron transfer remains unclear. Here, we investigated whether CET is involved in de novo NADP⁺ supply in Arabidopsis thaliana and measured chloroplastic NADP dynamics to evaluate responsiveness to variable light, photochemical inhibitors, darkness, and CET activity. The sum of oxidized and reduced forms shows that levels of NADP and NAD increase and decrease, respectively, in response to light; levels of NADP and NAD decrease and increase in the dark, respectively. Moreover, consistent with the pH change in the stroma, the pH preference of chloroplast NAD⁺ phosphorylation and NADP⁺ dephosphorylation is alkaline and weakly acidic, respectively. Furthermore, CET is correlated with upregulation of light-responsive NADP level increases and downregulation of dark-responsive NADP level reductions. These findings are consistent with CET helping to regulate NADP pool size via stromal pH regulation under fluctuating light conditions.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s41467-023-42995-9
  DOI ID：10.1038/s41467-023-42995-9, eISSN：2041-1723, ORCID：146070222
• MHP1 and MHL generate odd-chain fatty acids from 2-hydroxy fatty acids in sphingolipids and are related to immunity in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Marina Ushio; Toshiki Ishikawa; Takakazu Matsuura; Izumi C. Mori; Maki Kawai-Yamada; Yoichiro Fukao; Minoru Nagano
  Plant Science, Volume：336, First page：111840, Last page：111840, Nov. 2023, [Reviewed]
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.plantsci.2023.111840
  DOI ID：10.1016/j.plantsci.2023.111840, ISSN：0168-9452
• Heterologous expression of mtf and mtc genes of Pseudanabaena foetida var. intermedia is sufficient to produce 2-methylisoborneol in Escherichia coli.　　　　　　　　　　　　　　　
  Kaushalya Dayarathne; Toshiki Ishikawa; Satoru Watanabe; Yuuma Ishikawa; Kadeer Aikeranmu; Hina Kitagawaa; Natsumi Komatsubara; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Microbiology spectrum, Volume：11, Number：5, First page：e0256123, Sep. 2023, [Reviewed], ［International magazine］
  Microbial volatile metabolite 2-methylisoborneol (2-MIB) causes odor and taste issues in drinking water, making it unappealing for human consumption. It has been suggested that 2-MIB biosynthesis consists of two main steps, namely, methylation of geranyl diphosphate into 2-methyl geranyl diphosphate by geranyl diphosphate methyl transferase (GPPMT) and subsequent cyclization into 2-MIB by 2-MIB synthase (MIBS). Pseudanabaena foetida var. intermedia is a 2-MIB-producing cyanobacterium whose GPPMT and MIBS enzymes are encoded by adjacent mtf and mtc genes. The present study identified a 2-MIB-related gene cluster composed of cnbA, mtf, mtc, and cnbB genes in P. foetida var. intermedia. The two homologous cyclic nucleotide-binding protein genes, cnbA and cnbB, were detected adjacent to the mtf and mtc genes, respectively. The nucleotide sequence of the cnbA-mtf-mtc-cnbB gene cluster showed 99.55% identity with 2-MIB synthesis-associated gene cluster of Pseudanabaena sp. dqh15. RT-PCR results revealed that mtf and mtc genes are co-expressed, while cnbA and cnbB genes are expressed independently in P. foetida var. intermedia. To investigate whether only mtf and mtc genes are sufficient for 2-MIB synthesis, the two-gene unit (mtf-mtc) was introduced into Escherichia coli strain JM109 via overexpression vector pYS1C. Gas chromatograph-mass spectrometry results showed that the E. coli strain transformed with mtf-mtc was able to produce 2-MIB. The intracellular 2-MIB level in P. foetida var. intermedia was higher than the extracellular 2-MIB level, while the transformed E. coli strain showed an opposite trend. Growth inhibition was observed in the 2-MIB-producing transformed E. coli strain. IMPORTANCE Contamination of drinking water with odiferous microbial metabolite 2-MIB is a worldwide concern. Removal of 2-MIB from drinking water burdens the water purification process. Therefore, it is important to search for alternative methods, such as suppressing the production of 2-MIB by aquatic microorganisms. For that, it is necessary to expand the current knowledge about the mechanism of 2-MIB synthesis at the genetic level. This study revealed that mtf and mtc genes of the 2-MIB-related gene cluster are transcribed as a single unit in P. foetida var. intermedia, and the expression of both mtf and mtc genes is essential and sufficient for 2-MIB synthesis in E. coli heterologous gene expression system.
  English, Scientific journal
  DOI：https://doi.org/10.1128/spectrum.02561-23
  DOI ID：10.1128/spectrum.02561-23, PubMed ID：37732762, PubMed Central ID：PMC10580876
• NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence.　　　　　　　　　　　　　　　
  Isura Sumeda Priyadarshana Nagahage; Kohei Matsuda; Kyoko Miyashita; Sumire Fujiwara; Chanaka Mannapperuma; Takuya Yamada; Shingo Sakamoto; Toshiki Ishikawa; Minoru Nagano; Misato Ohtani; Ko Kato; Hirofumi Uchimiya; Nobutaka Mitsuda; Maki Kawai-Yamada; Taku Demura; Masatoshi Yamaguchi
  Plant direct, Volume：7, Number：9, First page：e529, Sep. 2023, [Reviewed], ［International magazine］
  The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.
  English, Scientific journal
  DOI：https://doi.org/10.1002/pld3.529
  DOI ID：10.1002/pld3.529, PubMed ID：37731912, PubMed Central ID：PMC10507225
• Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants　　　　　　　　　　　　　　　
  Chaomurilege; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Hideki Murayama; Maki Kawai-Yamada
  Plant Signaling and Behavior, Volume：18, Number：1, First page：e2215618, May 2023, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1080/15592324.2023.2215618
  DOI ID：10.1080/15592324.2023.2215618
• Chloroplastic Sec14-like proteins modulate growth and phosphate deficiency responses in Arabidopsis and rice
  Mailun Yang; Yasuhito Sakruaba; Toshiki Ishikawa; Namie Ohtsuki; Maki Kawai-Yamada; Shuichi Yanagisawa
  Plant Physiology, Apr. 2023, [Reviewed]
  Abstract
  
  Phosphorus is an essential nutrient acquired from soil as phosphate (Pi), and its deficiency severely reduces plant growth and crop yield. Here, we show that single nucleotide polymorphisms (SNPs) at the PHOSPHATIDYLINOSITOL TRANSFER PROTEIN7 (AtPITP7) locus, which encodes a chloroplastic Sec14-like protein, are associated with genetic diversity regarding Pi uptake activity in Arabidopsis (Arabidopsis thaliana). Inactivation of AtPITP7 and its rice (Oryza sativa) homolog (OsPITP6) through T-DNA insertion and CRISPR/Cas9-mediated gene editing, respectively, decreased Pi uptake and plant growth, regardless of Pi availability. By contrast, overexpression of AtPITP7 and OsPITP6 enhanced Pi uptake and plant growth, especially under limited Pi supply. Importantly, overexpression of OsPITP6 increased the tiller number and grain yield in rice. Targeted metabolome analysis of glycerolipids in leaves and chloroplasts revealed that inactivation of OsPITP6 alters phospholipid contents, independent of Pi availability, diminishing the reduction in phospholipid content and increase in glycolipid content induced by Pi deficiency; meanwhile, overexpression of OsPITP6 enhanced Pi deficiency-induced metabolic alterations. Together with transcriptome analysis of ospitp6 rice plants and phenotypic analysis of grafted Arabidopsis chimeras, these results suggest that chloroplastic Sec14-like proteins play an essential role in growth modulations in response to changes in Pi availability, although their function is critical for plant growth under any Pi condition. The superior traits of OsPITP6-overexpressing rice plants also highlight the potential of OsPITP6 and its homologs in other crops as additional tools for improving Pi uptake and plant growth in low Pi environments.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiad212
  DOI ID：10.1093/plphys/kiad212, ISSN：0032-0889, eISSN：1532-2548
• Loss of peroxisomal NAD kinase 3 (NADK3) affects photorespiration metabolism in Arabidopsis　　　　　　　　　　　　　　　
  Shota Suzuki; Daimu Tanaka; Atsuko Miyagi; Kentaro Takahara; Masaru Kono; Chaomurilege; Ko Noguchi; Toshiki Ishikawa; Minoru Nagano; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Journal of Plant Physiology, Volume：283, First page：153950, Mar. 2023, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2023.153950
  DOI ID：10.1016/j.jplph.2023.153950
• Dynamic seasonal changes in photosynthesis systems in leaves of Asarum tamaense, an evergreen understorey herbaceous species　　　　　　　　　　　　　　　
  Naoki Wada; Issei Kondo; Ryouichi Tanaka; Junko Kishimoto; Atsuko Miyagi; Maki Kawai-Yamada; Yusuke Mizokami; Ko Noguchi
  Annals of Botany, Volume：in press, Dec. 2022, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1093/aob/mcac156
  DOI ID：10.1093/aob/mcac156
• Arabidopsis nitrate-induced aspartate oxidase gene expression is necessary to maintain metabolic balance under nitrogen nutrient fluctuation
  Moriaki Saito; Mineko Konishi; Atsuko Miyagi; Yasuhito Sakuraba; Maki Kawai-Yamada; Shuichi Yanagisawa
  Communications Biology, Volume：5, Number：1, Dec. 2022, [Reviewed]
  Abstract
  
  Nitrate is a nutrient signal that regulates growth and development through NLP transcription factors in plants. Here we identify the L-aspartate oxidase gene (AO) necessary for de novo NAD⁺ biosynthesis as an NLP target in Arabidopsis. We investigated the physiological significance of nitrate-induced AO expression by expressing AO under the control of the mutant AO promoter lacking the NLP-binding site in the ao mutant. Despite morphological changes and severe reductions in fresh weight, the loss of nitrate-induced AO expression resulted in minimum effects on NAD(H) and NADP(H) contents, suggesting compensation of decreased de novo NAD⁺ biosynthesis by reducing the growth rate. Furthermore, metabolite profiling and transcriptome analysis revealed that the loss of nitrate-induced AO expression causes pronounced impacts on contents of TCA cycle- and urea cycle-related metabolites, gene expression profile, and their modifications in response to changes in the nitrogen nutrient condition. These results suggest that proper maintenance of metabolic balance requires the coordinated regulation of multiple metabolic pathways by NLP-mediated nitrate signaling in plants.
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1038/s42003-022-03399-5
  DOI ID：10.1038/s42003-022-03399-5, eISSN：2399-3642
• Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ
  Atsuko Miyagi; Kazuhisa Mori; Toshiki Ishikawa; Satoshi Ohkubo; Shunsuke Adachi; Masatoshi Yamaguchi; Taiichiro Ookawa; Toshihisa Kotake; Maki Kawai-Yamada
  Metabolomics, Volume：18, Number：12, Nov. 2022, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s11306-022-01958-9
  DOI ID：10.1007/s11306-022-01958-9, eISSN：1573-3890
• Loss of chloroplast-localized NAD kinase causes ROS stress in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Chaomurilege; Yanhui Zu; Atsuko Miyagi; Shin-Nosuke Hashida; Toshiki Ishikawa; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Journal of Plant Research, Volume：in press, Nov. 2022, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-022-01420-w
  DOI ID：10.1007/s10265-022-01420-w
• Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst
  Tomomi Ukawa; Fumihiko Banno; Toshiki Ishikawa; Kota Kasahara; Yuuta Nishina; Rika Inoue; Keigo Tsujii; Masatoshi Yamaguchi; Takuya Takahashi; Yoichiro Fukao; Maki Kawai-Yamada; Minoru Nagano
  Plant Physiology, Volume：189, Number：2, First page：839, Last page：857, Jun. 2022, [Reviewed]
  Abstract
  
  Plant sphingolipids mostly possess 2-hydroxy fatty acids (HFA), the synthesis of which is catalyzed by FA 2-hydroxylases (FAHs). In Arabidopsis (Arabidopsis thaliana), two FAHs (FAH1 and FAH2) have been identified. However, the functions of FAHs and sphingolipids with HFAs (2-hydroxy sphingolipids) are still unknown because of the lack of Arabidopsis lines with the complete deletion of FAH1. In this study, we generated a FAH1 mutant (fah1c) using CRISPR/Cas9-based genome editing. Sphingolipid analysis of fah1c, fah2, and fah1cfah2 mutants revealed that FAH1 hydroxylates very long-chain FAs (VLCFAs), whereas the substrates of FAH2 are VLCFAs and palmitic acid. However, 2-hydroxy sphingolipids are not completely lost in the fah1cfah2 double mutant, suggesting the existence of other enzymes catalyzing the hydroxylation of sphingolipid FAs. Plasma membrane (PM) analysis and molecular dynamics simulations revealed that hydroxyl groups of sphingolipid acyl chains play a crucial role in the organization of nanodomains, which are nanoscale liquid-ordered domains mainly formed by sphingolipids and sterols in the PM, through hydrogen bonds. In the PM of the fah1cfah2 mutant, the expression levels of 26.7% of the proteins, including defense-related proteins such as the pattern recognition receptors (PRRs) brassinosteroid insensitive 1-associated receptor kinase 1 and chitin elicitor receptor kinase 1, NADPH oxidase respiratory burst oxidase homolog D (RBOHD), and heterotrimeric G proteins, were lower than that in the wild-type. In addition, reactive oxygen species (ROS) burst was suppressed in the fah1cfah2 mutant after treatment with the pathogen-associated molecular patterns flg22 and chitin. These results indicated that 2-hydroxy sphingolipids are necessary for the organization of PM nanodomains and ROS burst through RBOHD and PRRs during pattern-triggered immunity.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiac134
  DOI ID：10.1093/plphys/kiac134, ISSN：0032-0889, eISSN：1532-2548
• VNI2 effectively inhibits transcriptional activities of VND7 through a conserved sequence　　　　　　　　　　　　　　　
  Aili Ailizati; Isura Sumeda Priyadarshana Nagahage; Atsuko Miyagi; Toshiki Ishikawa; Maki Kawai-Yamada; Taku Demura; Masatoshi Yamaguchi
  Plant Biotechnology, Volume：39, Number：2, First page：147, Last page：153, Jun. 2022, [Reviewed], ［Internationally co-authored］
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.22.0122a
  DOI ID：10.5511/plantbiotechnology.22.0122a
• Intraspecific interaction of host plants leads to concentrated distribution of a specialist herbivore through metabolic alterations in the leaves.
  Haruna Ohsaki; Atsuko Miyagi; Maki Kawai-Yamada; Akira Yamawo
  Functional Ecology, Volume：36, Number：3, First page：779, Last page：793, Mar. 2022, [Reviewed]
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/1365-2435.13988
  DOI ID：10.1111/1365-2435.13988, ISSN：0269-8463, eISSN：1365-2435
• An Arabidopsis NAC domain transcriptional activator VND7 negatively regulates VNI2 expression　　　　　　　　　　　　　　　
  Aili Ailizati; Isura Sumeda Priyadarshana Nagahage; Atsuko Miyagi; Toshiki Ishikawa; Maki Kawai-Yamada; Taku Demura; Masatoshi Yamaguchi
  Plant Biotechnology, Volume：38, Number：4, First page：415, Last page：420, Dec. 2021, [Reviewed], ［Internationally co-authored］
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.21.1013a
  DOI ID：10.5511/plantbiotechnology.21.1013a
• Change in expression levels of NAD kinase-encoding genes in Flaveria species　　　　　　　　　　　　　　　
  Masami Tanaka; Yuuma Ishikawa; Sayaka Suzuki; Takako Ogawa; Yukimi Y. Taniguchi; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yuri N. Munekage; Maki Kawai-Yamada
  Journal of Plant Physiology, Volume：265, First page：153495, Last page：153495, Oct. 2021
  Elsevier BV, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2021.153495
  DOI ID：10.1016/j.jplph.2021.153495, ISSN：0176-1617
• The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Cedric Cassan; Aikeranmu Kadeer; Koki Yuasa; Nozomu Sato; Kintake  Sonoike; Yasuko Kaneko; Atsuko Miyagi; Hiroko Takahashi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yoshitaka Nishiyama; Yukako Hihara; Yves Gibon; Maki Kawai-Yamada
  Plant and Cell Physiology, Volume：62, Number：4, First page：668, Last page：677, Sep. 2021, [Reviewed]
  
  NADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in the unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the Photosystem II (PSII) photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain the photosynthetic capacity.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab023
  DOI ID：10.1093/pcp/pcab023, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：33210985, PubMed Central ID：PMC7781788
• Reprogramming sphingolipid glycosylation is required for endosymbiont persistence in Medicago truncatula　　　　　　　　　　　　　　　
  William M. Moore; Candace Chan; Toshiki Ishikawa; Emilie A. Rennie; Heidi M.-L. Wipf; Veronica Benites; Maki Kawai-Yamada; Jenny C. Mortimer; Henrik V. Scheller
  Current Biology, Volume：31, Number：11, First page：2374, Last page：2385.e4, Jun. 2021
  Cell Press, English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2021.03.067
  DOI ID：10.1016/j.cub.2021.03.067, ISSN：1879-0445, PubMed ID：33857428, SCOPUS ID：85104939324
• The Arabidopsis thaliana nucleotide sugar transporter GONST2 is a functional homolog of GONST1.　　　　　　　　　　　　　　　
  Beibei Jing; Toshiki Ishikawa; Nicole Soltis; Noriko Inada; Yan Liang; Gosia Murawska; Lin Fang; Fekadu Andeberhan; Ramana Pidatala; Xiaolan Yu; Edward Baidoo; Maki Kawai-Yamada; Dominique Loque; Daniel J Kliebenstein; Paul Dupree; Jenny C Mortimer
  Plant direct, Volume：5, Number：3, First page：e00309, Mar. 2021, ［International magazine］
  Glycosylinositolphosphorylceramides (GIPCs) are the predominant lipid in the outer leaflet of the plasma membrane. Characterized GIPC glycosylation mutants have severe or lethal plant phenotypes. However, the function of the glycosylation is unclear. Previously, we characterized Arabidopsis thaliana GONST1 and showed that it was a nucleotide sugar transporter which provides GDP-mannose for GIPC glycosylation. gonst1 has a severe growth phenotype, as well as a constitutive defense response. Here, we characterize a mutant in GONST1's closest homolog, GONST2. The gonst2-1 allele has a minor change to GIPC headgroup glycosylation. Like other reported GIPC glycosylation mutants, gonst1-1gonst2-1 has reduced cellulose, a cell wall polymer that is synthesized at the plasma membrane. The gonst2-1 allele has increased resistance to a biotrophic pathogen Golovinomyces orontii but not the necrotrophic pathogen Botrytis cinerea. Expression of GONST2 under the GONST1 promoter can rescue the gonst1 phenotype, indicating that GONST2 has a similar function to GONST1 in providing GDP-D-Man for GIPC mannosylation.
  English, Scientific journal
  DOI：https://doi.org/10.1002/pld3.309
  DOI ID：10.1002/pld3.309, PubMed ID：33763627, PubMed Central ID：PMC7980081
• The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803.　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Cedric Cassan; Aikeranmu Kadeer; Koki Yuasa; Nozomu Sato; Kintake Sonoike; Yasuko Kaneko; Atsuko Miyagi; Hiroko Takahashi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yoshitaka Nishiyama; Yukako Hihara; Yves Gibon; Maki Kawai-Yamada
  Plant & cell physiology, Feb. 2021, ［Domestic magazine］
  NADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the PSII photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain photosynthetic capacity.
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab023
  DOI ID：10.1093/pcp/pcab023, PubMed ID：33560438
• Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment.　　　　　　　　　　　　　　　
  Yuichi Yokochi; Keisuke Yoshida; Florian Hahn; Atsuko Miyagi; Ken-Ichi Wakabayashi; Maki Kawai-Yamada; Andreas P M Weber; Toru Hisabori
  Proceedings of the National Academy of Sciences of the United States of America, Volume：118, Number：6, Feb. 2021, ［International magazine］
  Many enzymes involved in photosynthesis possess highly conserved cysteine residues that serve as redox switches in chloroplasts. These redox switches function to activate or deactivate enzymes during light-dark transitions and have the function of fine-tuning their activities according to the intensity of light. Accordingly, many studies on chloroplast redox regulation have been conducted under the hypothesis that "fine regulation of the activities of these enzymes is crucial for efficient photosynthesis." However, the impact of the regulatory system on plant metabolism is still unclear. To test this hypothesis, we here studied the impact of the ablation of a redox switch in chloroplast NADP-malate dehydrogenase (MDH). By genome editing, we generated a mutant plant whose MDH lacks one of its redox switches and is active even in dark conditions. Although NADPH consumption by MDH in the dark is expected to be harmful to plant growth, the mutant line did not show any phenotypic differences under standard long-day conditions. In contrast, the mutant line showed severe growth retardation under short-day or fluctuating light conditions. These results indicate that thiol-switch redox regulation of MDH activity is crucial for maintaining NADPH homeostasis in chloroplasts under these conditions.
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.2016903118
  DOI ID：10.1073/pnas.2016903118, PubMed ID：33531363
• Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation
  Kenji Nagata; Toshiki Ishikawa; Maki Kawai-Yamada; Taku Takahashi; Mitsutomo Abe
  Development, Volume：148, Number：2, First page：dev194969, Last page：dev194969, Jan. 2021
  The differentiation of distinct cell types in appropriate patterns is a fundamental process in the development of multicellular organisms. In Arabidopsis thaliana, protoderm/epidermis differentiates as a single cell layer at the outermost position. However, little is known about the molecular nature of the positional signals that achieve correct epidermal cell differentiation. Here, we propose that very-long-chain fatty acid-containing ceramides (VLCFA-Cers) mediate positional signals by stimulating the function of ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1), a master regulator of protoderm/epidermis differentiation, during lateral root development. We show that VLCFA-Cers, which are synthesized predominantly in the outermost cells, bind to the lipid-binding domain of ATML1. Importantly, this cell type-specific protein-lipid association alters the activity of ATML1 protein and consequently restricts its expression to the protoderm/epidermis through a transcriptional feedback loop. Furthermore, establishment of a compartment, enriched with VLCFA-containing sphingolipids, at the outer lateral membrane facing the external environment may function as a determinant of protodermal cell fate. Taken together, our results indicate that VLCFA-Cers play a pivotal role in directing protoderm/epidermis differentiation by mediating positional signals to ATML1.
  
  This article has an associated ‘The people behind the papers’ interview.
  The Company of Biologists, Scientific journal
  DOI：https://doi.org/10.1242/dev.194969
  DOI ID：10.1242/dev.194969, ISSN：0950-1991, eISSN：1477-9129
• Altered metabolism of chloroplastic NAD kinase-overexpressing Arabidopsis in response to magnesium sulfate supplementation.　　　　　　　　　　　　　　　
  Maki Kawai-Yamada; Atsuko Miyagi; Yuki Sato; Yuki Hosoi; Shin-Nosuke Hashida; Toshiki Ishikawa; Masatoshi Yamaguchi
  Plant signaling & behavior, Volume：16, Number：1, First page：1844509, Last page：1844509, Nov. 2020, ［International magazine］
  Nicotinamide adenine dinucleotide (NAD)/NAD phosphate (NADPH) is essential for numerous redox reactions and serve as co-factors in multiple metabolic processes in all organisms. NAD kinase (NADK) is an enzyme involved in the synthesis of NADP+ from NAD+ and ATP. Arabidopsis NADK2 (AtNADK2) is a chloroplast-localizing enzyme that provides recipients of reducing power in photosynthetic electron transfer. When Arabidopsis plants were grown on MS medium supplemented with 5 mM MgSO4, an AtNADK2-overexpressing line exhibited higher glutathione and total sulfur accumulation than control plants. Metabolomic analysis of major amino acids and organic acids using capillary electrophoresis-mass spectrometry demonstrated that overexpression of AtNADK2 affected a range of metabolic processes in response to MgSO4 supplementation.
  English, Scientific journal
  DOI：https://doi.org/10.1080/15592324.2020.1844509
  DOI ID：10.1080/15592324.2020.1844509, PubMed ID：33210985, PubMed Central ID：PMC7781788
• An Arabidopsis NAC domain transcription factor, ATAF2, promotes age-dependent and dark-induced leaf senescence.　　　　　　　　　　　　　　　
  Isura Sumeda Priyadarshana Nagahage; Shingo Sakamoto; Minoru Nagano; Toshiki Ishikawa; Nobutaka Mitsuda; Maki Kawai-Yamada; Masatoshi Yamaguchi
  Physiologia plantarum, Volume：170, Number：2, First page：299, Last page：308, Oct. 2020, ［International magazine］
  Leaf senescence is controlled developmentally and environmentally and is affected by numerous genes, including transcription factors. An Arabidopsis NAC domain transcription factor, ATAF2, is known to regulate biotic stress responses. Recently, we have demonstrated that ATAF2 upregulates ORE1, a key regulator of leaf senescence. Here, to investigate the function of ATAF2 in leaf senescence further, we generated and analyzed overexpressing transgenic and T-DNA inserted mutant lines. Transient expression analysis indicated that ATAF2 upregulates several NAC domain transcription factors that regulate senescence. Indeed, ATAF2 overexpression induced the expression of senescence-related genes, thereby accelerating leaf senescence, whereas the expression of such genes in ataf2 mutants was lower than that of wild-type plants. Furthermore, the ataf2 mutants exhibited significant delays in dark-induced leaf senescence. It was also found that ATAF2 induces the expression of transcription factors, which both promotes and represses leaf senescence. The present study demonstrates that ATAF2 promotes leaf senescence in response to developmental and environmental signals.
  English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13156
  DOI ID：10.1111/ppl.13156, PubMed ID：32579231
• Metabolome analysis of rice leaves to obtain low-oxalate strain from ion beam-mutagenised population.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Takuya Saimaru; Nozomi Harigai; Yutaka Oono; Yoshihiro Hase; Maki Kawai-Yamada
  Metabolomics : Official journal of the Metabolomic Society, Volume：16, Number：9, First page：94, Last page：94, Sep. 2020, ［International magazine］
  INTRODUCTION: Rice leaves and stems, which can be used as rice straw for livestock feed, accumulate soluble oxalate. The oxalate content often reaches 5% of the dry weight leaves. Excess uptake of oxalate-rich plants causes mineral deficiencies in vertebrates, so it is important to reduce the oxalate content in rice leaves to produce high-quality rice straw. However, the mechanism of oxalate accumulation in rice has remained unknown. OBJECTIVES: To understand metabolic networks relating oxalate accumulation in rice. METHODS: In this study, we performed metabolome analysis of rice M2 population generated by ion-beam irradiation using CE-MS. RESULTS: The result showed wide variation of oxalate contents in M2 plants compared with those of control plants. Multivariate analyses of metabolome dataset revealed that oxalate accumulation was strongly related with anionic compounds such as 2OG and succinate. For low-oxalate plants, four patterns of metabolic alterations affected oxalate contents in the M2 leaves were observed. In M3 plants, we found putative low-oxalate line obtained from low-oxalate M2 mutant. CONCLUSIONS: These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-020-01713-y
  DOI ID：10.1007/s11306-020-01713-y, PubMed ID：32894362
• Quantitative and Qualitative Analyses of Triacylglycerol Production in the Wild-Type Cyanobacterium Synechocystis sp. PCC 6803 and the Strain Expressing AtfA from Acinetobacter baylyi ADP1.　　　　　　　　　　　　　　　
  Motoki Tanaka; Toshiki Ishikawa; So Tamura; Yujiro Saito; Maki Kawai-Yamada; Yukako Hihara
  Plant & cell physiology, Volume：61, Number：9, First page：1537, Last page：1547, Sep. 2020, ［Domestic magazine］
  Although cyanobacteria do not possess wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT), the bacterial enzyme for triacylglycerol (TAG) production, there have been several studies reporting the accumulation of TAG-like compounds in cyanobacteria. In this study, we aimed to evaluate TAG productivity of the ΔrecJ::atfA strain of Synechocystis sp. PCC 6803 generated by inserting atfA encoding WS/DGAT from Acinetobacter baylyi ADP1 into recJ (sll1354), together with the wild type (WT) and the gene-disrupted strain of slr2103 having homology with eukaryotic DGAT2 gene family (Δ2103). Thin-layer chromatography (TLC) of neutral lipids or isolation of the neutral lipid-enriched fraction followed by gas chromatography or liquid chromatography-tandem mass spectrometry was employed for analyses. The ΔrecJ::atfA strain accumulated 0.508 nmol ml-1OD730-1 of TAG after a week of incubation at 100 μmol photons m-2 s-1. The saturated fatty acids C16:0 and C18:0 accounted for about 50% and 20% of the TAG fatty acids, respectively, suggesting that de novo-synthesized fatty acids were preferentially incorporated into TAG molecules. When the neutral lipid profile of the lipid extracts was examined by TLC, a spot located in a slightly lower position compared with the TAG standard was detected in WT but not in the Δ2103 strain. TAG accumulation levels of both strains was only 0.01-0.03 nmol ml-1OD730-1, but the fatty acid composition was substantially different from that of the background. These results suggest that trace amounts of TAG can be produced in Synechocystis cells by enzymes other than Slr2103, and major constituents of the TAG-like spot are unknown lipid species produced by Slr2103.
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcaa069
  DOI ID：10.1093/pcp/pcaa069, PubMed ID：32433767
• Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5 -B.　　　　　　　　　　　　　　　
  Minoru Nagano; Haruko Ueda; Yoichiro Fukao; Maki Kawai-Yamada; Ikuko Hara-Nishimura
  Plant signaling & behavior, Volume：15, Number：9, First page：1790196, Last page：1790196, Sep. 2020, ［International magazine］
  The endoplasmic reticulum (ER) is a multifunctional organelle that performs multiple cellular activities in eukaryotes. Visualizing ER using fluorescent proteins is a powerful method of analyzing its dynamics and to understand its functions. However, red fluorescent proteins with both an N-terminal signal peptide (SP) and a C-terminal ER retention tetrapeptide (HDEL) often cause mislocalization to vacuoles or extracellular spaces when they are constitutively expressed in Arabidopsis. To obtain a red fluorescent ER marker, we selected Arabidopsis cytochrome b5 -B (Cb5-B), a tail-anchored (TA) protein on the ER membrane. Its localization is determined by the transmembrane domain (TMD) and tail domain at the C-terminus. We fused the TMD and the tail domain of Cb5-B to the C-terminus of a red fluorescent protein, tdTomato (tdTomato-CTT). When tdTomato-CTT was constitutively expressed under the ubiquitin10 promoter in Arabidopsis, the fluorescent signal was exclusively detected at the ER by means of the reliable ER marker SP-GFP-HDEL. Therefore, tdTomato-CTT can accurately visualize the ER in stable Arabidopsis lines. Additionally, transient assays showed that tdTomato-CTT can also be used as an ER marker in onion, rice, and Nicotiana benthamiana. We believe that TA proteins could be used to generate various organellar membrane markers in plants.
  English, Scientific journal
  DOI：https://doi.org/10.1080/15592324.2020.1790196
  DOI ID：10.1080/15592324.2020.1790196, PubMed ID：32633191
• Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves.　　　　　　　　　　　　　　　
  Daisuke Takagi; Atsuko Miyagi; Youshi Tazoe; Mao Suganami; Maki Kawai-Yamada; Akihiro Ueda; Yuji Suzuki; Ko Noguchi; Naoki Hirotsu; Amane Makino
  Plant, cell & environment, Volume：43, Number：9, First page：2033, Last page：2053, Sep. 2020, [Reviewed], ［International magazine］
  Phosphorus (P) is an essential mineral nutrient for plants. Nevertheless, excessive P accumulation in leaf mesophyll cells causes necrotic symptoms in land plants; this phenomenon is termed P toxicity. However, the detailed mechanisms underlying P toxicity in plants have not yet been elucidated. This study aimed to investigate the molecular mechanism of P toxicity in rice. We found that under excessive inorganic P (Pi) application, Rubisco activation decreased and photosynthesis was inhibited, leading to lipid peroxidation. Although the defence systems against reactive oxygen species accumulation were activated under excessive Pi application conditions, the Cu/Zn-type superoxide dismutase activities were inhibited. A metabolic analysis revealed that excessive Pi application led to an increase in the cytosolic sugar phosphate concentration and the activation of phytic acid synthesis. These conditions induced mRNA expression of genes that are activated under metal-deficient conditions, although metals did accumulate. These results suggest that P toxicity is triggered by the attenuation of both photosynthesis and metal availability within cells mediated by phytic acid accumulation. Here, we discuss the whole phenomenon of P toxicity, beginning from the accumulation of Pi within cells to death in land plants.
  English, Scientific journal
  DOI：https://doi.org/10.1111/pce.13772
  DOI ID：10.1111/pce.13772, ORCID：71982429, PubMed ID：32281116
• Dehydroascorbate Reductases and Glutathione Set a Threshold for High-Light-Induced Ascorbate Accumulation.　　　　　　　　　　　　　　　
  Yusuke Terai; Hiromi Ueno; Takahisa Ogawa; Yoshihiro Sawa; Atsuko Miyagi; Maki Kawai-Yamada; Takahiro Ishikawa; Takanori Maruta
  Plant physiology, Volume：183, Number：1, First page：112, Last page：122, May 2020, [Reviewed], ［International magazine］
  Plants require a high concentration of ascorbate as a redox buffer for survival under stress conditions, such as high light. Dehydroascorbate reductases (DHARs) are enzymes that catalyze the reduction of DHA to ascorbate using reduced glutathione (GSH) as an electron donor, allowing rapid ascorbate recycling. However, a recent study using an Arabidopsis (Arabidopsis thaliana) triple mutant lacking all three DHAR genes (herein called ∆dhar) did not find evidence for their role in ascorbate recycling under oxidative stress. To further study the function of DHARs, we generated ∆dhar Arabidopsis plants as well as a quadruple mutant line combining ∆dhar with an additional vtc2 mutation that causes ascorbate deficiency. Measurements of ascorbate in these mutants under low- or high-light conditions indicated that DHARs have a nonnegligible impact on full ascorbate accumulation under high light, but that they are dispensable when ascorbate concentrations are low to moderate. Because GSH itself can reduce DHA nonenzymatically, we used the pad2 mutant that contains ∼30% of the wild-type GSH level. The pad2 mutant accumulated ascorbate at a wild-type level under high light; however, when the pad2 mutation was combined with ∆dhar, there was near-complete inhibition of high-light-dependent ascorbate accumulation. The lack of ascorbate accumulation was consistent with a marked increase in the ascorbate degradation product threonate. These findings indicate that ascorbate recycling capacity is limited in ∆dhar pad2 plants, and that both DHAR activity and GSH content set a threshold for high-light-induced ascorbate accumulation.
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.19.01556
  DOI ID：10.1104/pp.19.01556, PubMed ID：32205453, PubMed Central ID：PMC7210653
• Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay　　　　　　　　　　　　　　　
  Yuma Ishikawa; Maki Kawai-Yamada; Shin-nosuke Hashida
  BIO-PROTOCOL, Volume：10, Number：1, 2020
  Bio-Protocol, {LLC}, English, Scientific journal
  DOI：https://doi.org/10.21769/bioprotoc.3480
  DOI ID：10.21769/bioprotoc.3480, ISSN：2331-8325, ORCID：83058164
• Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity.　　　　　　　　　　　　　　　
  Masaya Sato; Minoru Nagano; Song Jin; Atsuko Miyagi; Masatoshi Yamaguchi; Maki Kawai-Yamada; Toshiki Ishikawa
  Plants (Basel, Switzerland), Volume：9, Number：1, First page：19, Dec. 2019, [Reviewed], ［International magazine］
  Cis/trans isomerism of the Δ8 unsaturation of long-chain base (LCB) is found only in plant sphingolipids. This unique geometry is generated by sphingolipid LCB Δ8 desaturase SLD which produces both isomers at various ratios, resulting in diverse cis/trans ratios in plants. However, the biological significance of this isomeric diversity remains controversial. Here, we show that the plant-specific cis unsaturation of LCB selectively contributes to glucosylceramide (GlcCer)-dependent tolerance to aluminum toxicity. We established three transgenic rice lines with altered LCB unsaturation profiles. Overexpression of SLD from rice (OsSLD-OX), which preferentially exhibits cis-activity, or Arabidopsis (AtSLD-OX), showing preference for trans-activity, facilitated Δ8 unsaturation in different manners: a slight increase of cis-unsaturated glycosylinositolphosphoceramide (GIPC) in OsSLD-OX, and a drastic increase of trans-unsaturated GlcCer and GIPC in AtSLD-OX. Disruption of LCB Δ4 desaturase (des) significantly decreased the content of GlcCer. Fluorescence imaging analysis revealed that OsSLD-OX and AtSLD-OX showed increased plasma membrane fluidity, whereas des had less fluidity, demonstrating that the isomers universally contributed to increasing membrane fluidity. However, the results of a hydroponic assay showed decreased aluminum tolerance in AtSLD-OX and des compared to OsSLD-OX and the control plants, which did not correlate with membrane fluidity. These results suggest that cis-unsaturated GlcCer, not GIPC, selectively serves to maintain the membrane fluidity specifically associated with aluminum tolerance.
  English, Scientific journal
  DOI：https://doi.org/10.3390/plants9010019
  DOI ID：10.3390/plants9010019, PubMed ID：31877922, PubMed Central ID：PMC7020186
• Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation.　　　　　　　　　　　　　　　
  Rumana Yesmin Hasi; Makoto Miyagi; Katsuya Morito; Toshiki Ishikawa; Maki Kawai-Yamada; Hiroyuki Imai; Tatsuya Fukuta; Kentaro Kogure; Kaori Kanemaru; Junji Hayashi; Ryushi Kawakami; Tamotsu Tanaka
  Journal of biochemistry, Volume：166, Number：5, First page：441, Last page：448, Nov. 2019, [Reviewed], ［International magazine］
  Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipid in plants and fungi. Recently, we detected GIPC-specific phospholipase D (GIPC-PLD) activity in plants. Here, we found that GIPC-PLD activity in young cabbage leaves catalyzes transphosphatidylation. The available alcohol for this reaction is a primary alcohol with a chain length below C4. Neither secondary alcohol, tertiary alcohol, choline, serine nor glycerol serves as an acceptor for transphosphatidylation of GIPC-PLD. We also found that cabbage GIPC-PLD prefers GIPC containing two sugars. Neither inositol phosphoceramide, mannosylinositol phosphoceramide nor GIPC with three sugar chains served as substrate. GIPC-PLD will become a useful catalyst for modification of polar head group of sphingophospholipid.
  English, Scientific journal
  DOI：https://doi.org/10.1093/jb/mvz056
  DOI ID：10.1093/jb/mvz056, ISSN：0021-924X, PubMed ID：31504617
• High-yielding rice Takanari has superior photosynthetic response to a commercial rice Koshihikari under fluctuating light.　　　　　　　　　　　　　　　
  Shunsuke Adachi; Yu Tanaka; Atsuko Miyagi; Makoto Kashima; Ayumi Tezuka; Yoshihiro Toya; Shunzo Kobayashi; Satoshi Ohkubo; Hiroshi Shimizu; Maki Kawai-Yamada; Rowan F Sage; Atsushi J Nagano; Wataru Yamori
  Journal of experimental botany, Volume：70, Number：19, First page：5287, Last page：5297, Oct. 2019, [Reviewed], ［International magazine］
  Leaves within crop canopies experience variable light over the course of a day, which greatly affects photosynthesis and crop productivity. Little is known about the mechanisms of the photosynthetic response to fluctuating light and their genetic control. Here, we examined gas exchange, metabolite levels, and chlorophyll fluorescence during the photosynthetic induction response in an Oryza sativa indica cultivar with high yield (Takanari) and a japonica cultivar with lower yield (Koshihikari). Takanari had a faster induction response to sudden increases in light intensity than Koshihikari, as demonstrated by faster increases in net CO2 assimilation rate, stomatal conductance, and electron transport rate. In a simulated light regime that mimicked a typical summer day, the faster induction response in Takanari increased daily CO2 assimilation by 10%. The faster response of Takanari was explained in part by its maintenance of a larger pool of Calvin-Benson cycle metabolites. Together, the rapid responses of electron transport rate, metabolic flux, and stomatal conductance in Takanari contributed to the greater daily carbon gain under fluctuating light typical of natural environments.
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erz304
  DOI ID：10.1093/jxb/erz304, ISSN：0022-0957, PubMed ID：31257443, PubMed Central ID：PMC6793460
• Excessive assimilation of ammonium by plastidic glutamine synthetase is a major cause of ammonium toxicity in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Hachiya T; Inaba J; Wakazaki M; Sato M; Toyooka K; Miyagi A; Kawai-Yamada M; Kiba T; Gojon A; Sakakibara H
  bioRxiv, Volume：https://doi.org/10.1101/764324, Sep. 2019
  English, Scientific journal
• Oxalate contents in leaves of two rice cultivars grown at a free-air CO₂ enrichment (FACE) site　　　　　　　　　　　　　　　
  Atsuko Miyagi; Ko Noguchi; Takeshi Tokida; Yasuhiro Usui; Hirofumi Nakamura; Hidemitsu Sakai; Toshihiro Hasegawa; Maki Kawai-Yamada
  Plant Production Science, Volume：22, Number：3, First page：407, Last page：411, Jul. 2019, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1080/1343943X.2019.1598272
  DOI ID：10.1080/1343943X.2019.1598272
• Mitochondrial AOX Supports Redox Balance of Photosynthetic Electron Transport, Primary Metabolite Balance, and Growth in Arabidopsis thaliana under High Light.　　　　　　　　　　　　　　　
  Jiang Z; Watanabe CKA; Miyagi A; Kawai-Yamada M; Terashima I; Noguchi K
  International journal of molecular sciences, Volume：20, Number：12, Jun. 2019, [Reviewed]
  DOI：https://doi.org/10.3390/ijms20123067
  DOI ID：10.3390/ijms20123067, PubMed ID：31234590
• One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803.　　　　　　　　　　　　　　　
  Ishikawa Y; Miyagi A; Ishikawa T; Nagano M; Yamaguchi M; Hihara Y; Kaneko Y; Kawai-Yamada M
  The Plant journal : for cell and molecular biology, Volume：98, Number：4, First page：654, Last page：666, May 2019, [Reviewed], ［International magazine］
  Pyridine nucleotides (NAD(P)(H)) are electron carriers that are the driving forces in various metabolic pathways. Phosphorylation of NAD(H) to NADP(H) is performed by the enzyme NAD kinase (NADK). Synechocystis sp. PCC 6803 harbors two genes (sll1415 and slr0400) that encode proteins with NADK homology. When genetic mutants for sll1415 and slr0400 (Δ1415 and Δ0400, respectively) were cultured under photoheterotrophic growth conditions only the Δ1415 cells showed a growth defect. In wild-type cells, the sll1415 transcript accumulated after the cells were transferred to photoheterotrophic conditions. Furthermore, NAD(P)(H) measurements demonstrated that a dynamic metabolic conversion was implemented during the adaptation from photoautotrophic to photoheterotrophic conditions. Electron microscopy observation and biochemistry quantification demonstrated the accumulation of glycogen in the Δ1415 cells under photoheterotrophic conditions at 96 h. Quantitative real-time reverse transcription PCR (qRT-PCR) demonstrated the accumulation of mRNAs that encoded glycogen biosynthesis-related enzymes in photoheterotrophic Δ1415 cells. At 96 h, enzyme activity measurement in the photoheterotrophic Δ1415 cells demonstrated that the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were decreased, but the activities of glucose dehydrogenase were increased. Furthermore, metabolomics analysis demonstrated that the Δ1415 cells showed increased glucose-6-phosphate and 6-phosphogluconate content at 96 h. Therefore, sll1415 has a significant function in the oxidative pentose phosphate (OPP) pathway for catabolism of glucose under photoheterotrophic conditions. Additionally, it is presumed that the slr0400 had a different role in glucose catabolism during growth. These results suggest that the two Synechocystis sp. PCC 6803 NADKs (Sll1415 and Slr0400) have distinct functions in photoheterotrophic cyanobacterial metabolism.
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.14262
  DOI ID：10.1111/tpj.14262, ISSN：0960-7412, PubMed ID：30693583
• Detection of Disulfides in Protein Extracts of Arabidopsis thaliana Using Monobromobimane (mBB)　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Maki Kawai-Yamada
  BIO-PROTOCOL, Volume：9, Number：5, 2019
  Bio-Protocol, {LLC}, English, Scientific journal
  DOI：https://doi.org/10.21769/bioprotoc.3183
  DOI ID：10.21769/bioprotoc.3183, ISSN：2331-8325, ORCID：83058156
• Metabolic and biochemical responses of Potamogeton anguillanus Koidz. (Potamogetonaceae) to low oxygen conditions.　　　　　　　　　　　　　　　
  Parveen M; Miyagi A; Kawai-Yamada M; Rashid MH; Asaeda T
  Journal of plant physiology, Volume：232, First page：171, Last page：179, Jan. 2019, [Reviewed]
  DOI：https://doi.org/10.1016/j.jplph.2018.11.023
  DOI ID：10.1016/j.jplph.2018.11.023, ISSN：0176-1617, PubMed ID：30537604
• Arabidopsis Bax inhibitor-1 interacts with enzymes related to very-long-chain fatty acid synthesis.　　　　　　　　　　　　　　　
  Nagano M; Kakuta C; Fukao Y; Fujiwara M; Uchimiya H; Kawai-Yamada M
  Journal of plant research, Volume：132, Number：1, First page：131, Last page：143, Jan. 2019, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s10265-018-01081-8
  DOI ID：10.1007/s10265-018-01081-8, ISSN：0918-9440, eISSN：1618-0860, PubMed ID：30604175
• Physiological Significance of NAD Kinases in Cyanobacteria.　　　　　　　　　　　　　　　
  Ishikawa Y; Kawai-Yamada M
  Frontiers in plant science, Volume：10, First page：847, 2019, [Reviewed]
  DOI：https://doi.org/10.3389/fpls.2019.00847
  DOI ID：10.3389/fpls.2019.00847, PubMed ID：31316540
• Inter-Organelle NAD Metabolism Underpinning Light Responsive NADP Dynamics in Plants.　　　　　　　　　　　　　　　
  Hashida SN; Kawai-Yamada M
  Frontiers in plant science, Volume：10, First page：960, 2019, [Reviewed]
  DOI：https://doi.org/10.3389/fpls.2019.00960
  DOI ID：10.3389/fpls.2019.00960, PubMed ID：31404160
• Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803.　　　　　　　　　　　　　　　
  Yuta Kodama; Akihito Kawahara; Atsuko Miyagi; Toshiki Ishikawa; Maki Kawai-Yamada; Yasuko Kaneko; Yasushi Takimura; Yukako Hihara
  Biotechnology and Bioengineering, Volume：115, Number：12, First page：2974, Last page：2985, Dec. 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1002/bit.26842
  DOI ID：10.1002/bit.26842, ISSN：1097-0290
• A homologue of dihydrosphingosine C4 hydroxylase gene family member, DSH5, that shows spatial expression in rice; its ectopic expression leads to a lethal phenotype.　　　　　　　　　　　　　　　
  Tomohiro Imamura; Chihiro Obata; Kazuyoshi Yoneyama; Masatoshi Ichikawa; Akane Ikura; Hiromi Mutsuro-Aoki; Toshiki Ishikawa; Maki Kawai-Yamada; Tadamasa Sasaki; Hiroaki Kusano; Hiroaki Shimada
  GENES & GENETIC SYSTEMS, Volume：93, First page：135, Last page：142, Oct. 2018, [Reviewed]
  English
  DOI：https://doi.org/10.1266/ggs/17-00054
  DOI ID：10.1266/ggs/17-00054
• An NAC domain transcription factor ATAF2 acts as transcriptional activator or repressor dependent on promoter context　　　　　　　　　　　　　　　
  Isura Sumeda Priyadarshana Nagahage; Shingo Sakamoto; Minoru Nagano; Toshiki Ishikawa; Maki Kawai-Yamada; Nobutaka Mitsuda; Masatoshi Yamaguchi
  Plant Biotechnology, Volume：35, Number：3, First page：285, Last page：289, Sep. 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.18.0507a
  DOI ID：10.5511/plantbiotechnology.18.0507a
• Ferredoxin/thioredoxin system plays an important role in the chloroplastic NADP status of Arabidopsis.　　　　　　　　　　　　　　　
  Hashida SN; Miyagi A; Nishiyama M; Yoshida K; Hisabori T; Kawai-Yamada M
  The Plant journal : for cell and molecular biology, Volume：95, Number：6, First page：947, Last page：960, Sep. 2018, [Reviewed]
  DOI：https://doi.org/10.1111/tpj.14000
  DOI ID：10.1111/tpj.14000, ISSN：0960-7412, PubMed ID：29920827
• DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically.　　　　　　　　　　　　　　　
  Imamura T; Obata C; Yoneyama K; Ichikawa M; Ikura A; Mutsuro-Aoki H; Ishikawa T; Kawai-Yamada M; Sasaki T; Kusano H; Shimada H
  Genes & genetic systems, Volume：93, Number：4, First page：135, Last page：142, Sep. 2018, [Reviewed], ［Domestic magazine］
  Dihydrosphingosine C4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively. Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species. These DSHs showed similarity in their functions and complemented the yeast sur2D mutation. In contrast, homologs of DSH3 and DSH5 were found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs. DSH3 and DSH5 showed low-level expression. DSH5 expression was specifically in vascular bundle tissues. Ectopic expression of DSH5 induced a dwarf phenotype characterized by severe growth inhibition and an increase in the thickness of the leaf body caused by enlargement of bulliform cells in the leaves. However, no significant difference was observed in the amount of sphingolipid species. DSH5 did not complement the yeast sur2D mutation, implying that DSH5 has little effect on sphingolipid metabolism. These findings suggested that DSH3 and DSH5 originated and diverged in monocot plants.
  English, Scientific journal
  DOI：https://doi.org/10.1266/ggs.17-00054
  DOI ID：10.1266/ggs.17-00054, ISSN：1341-7568, PubMed ID：30185720
• Suppression of Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan-II.　　　　　　　　　　　　　　　
  Sechet J; Htwe S; Urbanowicz B; Agyeman A; Feng W; Ishikawa T; Colomes M; Kumar KS; Kawai-Yamada M; Dinneny JR; O'Neill MA; Mortimer JC
  The Plant journal : for cell and molecular biology, Sep. 2018, [Reviewed]
  DOI：https://doi.org/10.1111/tpj.14088
  DOI ID：10.1111/tpj.14088, ISSN：0960-7412, PubMed ID：30203879
• Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803.　　　　　　　　　　　　　　　
  Kodama Y; Kawahara A; Miyagi A; Ishikawa T; Kawai-Yamada M; Kaneko Y; Takimura Y; Hihara Y
  Biotechnology and bioengineering, Sep. 2018, [Reviewed]
  DOI：https://doi.org/10.1002/bit.26842
  DOI ID：10.1002/bit.26842, ISSN：0006-3592, PubMed ID：30252943
• GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.　　　　　　　　　　　　　　　
  Ishikawa T; Fang L; Rennie EA; Sechet J; Yan J; Jing B; Moore W; Cahoon EB; Scheller HV; Kawai-Yamada M; Mortimer JC
  Plant physiology, Volume：177, Number：3, First page：938, Last page：952, Jul. 2018, [Reviewed]
  DOI：https://doi.org/10.1104/pp.18.00396
  DOI ID：10.1104/pp.18.00396, ISSN：0032-0889, PubMed ID：29760197
• Effects of Elevated Atmospheric CO 2 on Respiratory Rates in Mature Leaves of Two Rice Cultivars Grown at a Free-Air CO 2 Enrichment Site and Analyses of the Underlying Mechanisms　　　　　　　　　　　　　　　
  Ko Noguchi; Tomonori Tsunoda; Atsuko Miyagi; Maki Kawai-Yamada; Daisuke Sugiura; Shin-Ichi Miyazawa; Takeshi Tokida; Yasuhiro Usui; Hirofumi Nakamura; Hidemitsu Sakai; Toshihiro Hasegawa
  Plant and Cell Physiology, Volume：59, Number：3, First page：637, Last page：649, Mar. 2018, [Reviewed]
  Oxford University Press, English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcy017
  DOI ID：10.1093/pcp/pcy017, ISSN：1471-9053, PubMed ID：29401364, SCOPUS ID：85043253711
• Evaluation of metabolic changes in oxalate-rich plant Rumex obtusifolius L. caused by ion beam irradiation.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Sayaka Kitano; Yutaka Oono; Yoshihiro Hase; Issay Narumi; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  Plant physiology and biochemistry : PPB, Volume：122, First page：40, Last page：45, Jan. 2018, [Reviewed], ［International magazine］
  Some Rumex species such as sorrel are edible as baby leaf salad greens. On the other hand, Rumex plants accumulate soluble oxalate, a toxic metabolite which causes serious diseases such as renal syndrome. We attempted to produce low-oxalate plants of R. obtusifolius, a perennial weed which has higher vitamin C and amino acid content and higher tolerance to stress than many other Rumex species. Ion beams are ionising radiation with high linear energy transfer that causes a wide spectrum of mutations. Thus, in the present study we evaluated the effects of ion beams on oxalate and other primary metabolites in leaves of R. obtusifolius using CE-MS. The results showed that oxalate content was increased by irradiation with carbon ion beams. Metabolome analysis revealed that ion beams affected carbon flow to the isocitrate pathway, which is involved in oxalate synthesis. These observations suggested that modulation of carbon flow to the isocitrate pathway is important to regulate oxalate levels in plants.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.plaphy.2017.11.001
  DOI ID：10.1016/j.plaphy.2017.11.001, ISSN：0981-9428, PubMed ID：29172104
• Synergistic effects of light quality, carbon dioxide and nutrients on metabolite compositions of head lettuce under artificial growth conditions mimicking a plant factory　　　　　　　　　　　　　　　
  Atsuko Miyagi; Hirofumi Uchimiya; Maki Kawai-Yamada
  FOOD CHEMISTRY, Volume：218, First page：561, Last page：568, Mar. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.foodchem.2016.09.102
  DOI ID：10.1016/j.foodchem.2016.09.102, ISSN：0308-8146, eISSN：1873-7072, ORCID：45646351, Web of Science ID：WOS:000386409700073
• CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics　　　　　　　　　　　　　　　
  Mayumi Sato; Atsuko Miyagi; Shozo Yoneyama; Seiki Gisusi; Yoshihiko Tokuji; Maki Kawai-Yamada
  Bioscience, Biotechnology and Biochemistry, Volume：81, Number：12, First page：2314, Last page：2322, 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1080/09168451.2017.1387049
  DOI ID：10.1080/09168451.2017.1387049, ISSN：1347-6947, ORCID：45646350, PubMed ID：29050513, SCOPUS ID：85038389198, Web of Science ID：WOS:000423159400012
• Loss of Inositol Phosphorylceramide Sphingolipid Mannosylation Induces Plant Immune Responses and Reduces Cellulose Content in Arabidopsis　　　　　　　　　　　　　　　
  Lin Fang; Toshiki Ishikawa; Emilie A. Rennie; Gosia M. Murawska; Jeemeng Lao; Jingwei Yan; Alex Yi-Lin Tsai; Edward E. K. Baidoo; Jun Xu; Jay D. Keasling; Taku Demura; Maki Kawai-Yamada; Henrik V. Scheller; Jenny C. Mortimer
  PLANT CELL, Volume：28, Number：12, First page：2991, Last page：3004, Dec. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.16.00186
  DOI ID：10.1105/tpc.16.00186, ISSN：1040-4651, eISSN：1532-298X, ORCID：45646353, PubMed ID：27895225, Web of Science ID：WOS:000393167800009
• Molecular characterization and targeted quantitative profiling of the sphingolipidome in rice　　　　　　　　　　　　　　　
  Toshiki Ishikawa; Yukihiro Ito; Maki Kawai-Yamada
  PLANT JOURNAL, Volume：88, Number：4, First page：681, Last page：693, Nov. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.13281
  DOI ID：10.1111/tpj.13281, ISSN：0960-7412, eISSN：1365-313X, ORCID：45646355, Web of Science ID：WOS:000389928200013
• Increased Rate of NAD Metabolism Shortens Plant Longevity by Accelerating Developmental Senescence in Arabidopsis　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Taketo Itami; Kentaro Takahara; Takayuki Hirabayashi; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANT AND CELL PHYSIOLOGY, Volume：57, Number：11, First page：2427, Last page：2439, Nov. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcw155
  DOI ID：10.1093/pcp/pcw155, ISSN：0032-0781, eISSN：1471-9053, ORCID：45646352, Web of Science ID：WOS:000393158000017
• Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp PCC 6803　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Atsuko Miyagi; Yuto Haishima; Toshiki Ishikawa; Minoru Nagano; Masatoshi Yamaguchi; Yukako Hihara; Maki Kawai-Yamada
  JOURNAL OF PLANT PHYSIOLOGY, Volume：205, First page：105, Last page：112, Oct. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2016.09.002
  DOI ID：10.1016/j.jplph.2016.09.002, ISSN：0176-1617, eISSN：1618-1328, ORCID：45646354, PubMed ID：27657983, Web of Science ID：WOS:000385858600014
• Plasma Membrane Microdomains Are Essential for Rac1-RbohB/H-Mediated Immunity in Rice　　　　　　　　　　　　　　　
  Minoru Nagano; Toshiki Ishikawa; Masayuki Fujiwara; Yoichiro Fukao; Yoji Kawano; Maki Kawai-Yamada; Ko Shimamoto
  PLANT CELL, Volume：28, Number：8, First page：1966, Last page：1983, Aug. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.16.00201
  DOI ID：10.1105/tpc.16.00201, ISSN：1040-4651, eISSN：1532-298X, ORCID：45646356, Web of Science ID：WOS:000386169200016
• KONJAC1 and 2 Are Key Factors for GDP-Mannose Generation and Affect L-Ascorbic Acid and Glucomannan Biosynthesis in Arabidopsis　　　　　　　　　　　　　　　
  Shota Sawake; Noriaki Tajima; Jenny C. Mortimer; Jeemeng Lao; Toshiki Ishikawa; Xiaolan Yu; Yukiko Yamanashi; Yoshihisa Yoshimi; Maki Kawai-Yamada; Paul Dupree; Yoichi Tsumuraya; Toshihisa Kotake
  PLANT CELL, Volume：27, Number：12, First page：3397, Last page：3409, Dec. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.15.00379
  DOI ID：10.1105/tpc.15.00379, ISSN：1040-4651, eISSN：1532-298X, ORCID：45646361, Web of Science ID：WOS:000368297100010
• Glycosylated sphingolipid biosynthesis and function in Arabidopsis
  Jenny C. Mortimer; Toshiki Ishikawa; Lin Fang; Beibei Jing; Emilie Rennie; Noriko Inada; Xiaolan Yu; Jeemeng Lao; Taku Demura; Maki Kawai-Yamada; Henrik Scheller; Paul Dupree
  GLYCOBIOLOGY, Volume：25, Number：11, First page：1266, Last page：1267, Nov. 2015, [Reviewed]
  English
  ISSN：0959-6658, eISSN：1460-2423, ORCID：45646360, Web of Science ID：WOS:000362991500108
• Overexpression of BAX INHIBITOR-1 Links Plasma Membrane Microdomain Proteins to Stress　　　　　　　　　　　　　　　
  Toshiki Ishikawa; Toshihiko Aki; Shuichi Yanagisawa; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANT PHYSIOLOGY, Volume：169, Number：2, First page：1333, Last page：1343, Oct. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.15.00445
  DOI ID：10.1104/pp.15.00445, ISSN：0032-0889, eISSN：1532-2548, ORCID：45646363, Web of Science ID：WOS:000365401000038
• Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots.　　　　　　　　　　　　　　　
  Takaki Yamauchi; Katsuhiro Shiono; Minoru Nagano; Aya Fukazawa; Miho Ando; Itsuro Takamure; Hitoshi Mori; Naoko K Nishizawa; Maki Kawai-Yamada; Nobuhiro Tsutsumi; Kiyoaki Kato; Mikio Nakazono
  Plant physiology, Volume：169, Number：1, First page：180, Last page：93, Sep. 2015, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.15.00106
  DOI ID：10.1104/pp.15.00106, ORCID：45646359, PubMed ID：26036614, PubMed Central ID：PMC4577372, Web of Science ID：WOS:000360930600017
• Effects of water turbulence on variations in cell ultrastructure and metabolism of amino acids in the submersed macrophyte, Elodea nuttallii (Planch.) H. St. John　　　　　　　　　　　　　　　
  K. S. S. Atapaththu; A. Miyagi; K. Atsuzawa; Y. Kaneko; M. Kawai-Yamada; T. Asaeda
  PLANT BIOLOGY, Volume：17, Number：5, First page：997, Last page：1004, Sep. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/plb.12346
  DOI ID：10.1111/plb.12346, ISSN：1435-8603, eISSN：1438-8677, ORCID：45646358, Web of Science ID：WOS:000359604800009
• Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Isura Sumeda Priyadarshana Nagahage; Misato Ohtani; Toshiki Ishikawa; Hirofumi Uchimiya; Maki Kawai-Yamada; Taku Demura
  PLANT BIOTECHNOLOGY, Volume：32, Number：2, First page：119, Last page：U14, Jun. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.15.0208a
  DOI ID：10.5511/plantbiotechnology.15.0208a, ISSN：1342-4580, ORCID：45646357, Web of Science ID：WOS:000356970600002
• The effects of dark incubation on cellular metabolism of the wild type cyanobacterium Synechocystis sp. PCC 6803 and a mutant lacking the transcriptional regulator cyAbrB2.　　　　　　　　　　　　　　　
  Masamitsu Hanai; Yusuke Sato; Atsuko Miyagi; Maki Kawai-Yamada; Kyoko Tanaka; Yasuko Kaneko; Yoshitaka Nishiyama; Yukako Hihara
  Life, Volume：4, Number：4, First page：770, Last page：787, Nov. 2014, [Reviewed], [Invited]
  English, Scientific journal
  DOI：https://doi.org/10.3390/life4040770
  DOI ID：10.3390/life4040770
• Does the Upstream Region Possessing MULE-Like Sequence in Rice Upregulate PsbS1 Gene Expression?　　　　　　　　　　　　　　　
  Mohammed Nuruzzaman; Tatsuo Kanno; Rika Amada; Yoshiki Habu; Ichiro Kasajima; Toshiki Ishikawa; Maki Kawai-Yamada; Hirofumi Uchimiya
  PLOS ONE, Volume：9, Number：9, Sep. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0102742
  DOI ID：10.1371/journal.pone.0102742, ISSN：1932-6203, ORCID：45646368, Web of Science ID：WOS:000342685600002
• Effects of NAD kinase 2 overexpression on primary metabolite profiles in rice leaves under elevated carbon dioxide　　　　　　　　　　　　　　　
  Y. Onda; A. Miyagi; K. Takahara; H. Uchimiya; M. Kawai-Yamada
  PLANT BIOLOGY, Volume：16, Number：4, First page：819, Last page：824, Jul. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/plb.12131
  DOI ID：10.1111/plb.12131, ISSN：1435-8603, eISSN：1438-8677, ORCID：45646369, Web of Science ID：WOS:000337613000016
• Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes　　　　　　　　　　　　　　　
  Minoru Nagano; Toshiki Ishikawa; Yoshie Ogawa; Mitsuru Iwabuchi; Akari Nakasone; Ko Shimamoto; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANTA, Volume：240, Number：1, First page：77, Last page：89, Jul. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-014-2065-7
  DOI ID：10.1007/s00425-014-2065-7, ISSN：0032-0935, eISSN：1432-2048, ORCID：45646364, Web of Science ID：WOS:000338318300005
• Development of an LC-MS/MS Method for the Analysis of Free Sphingoid Bases Using 4-Fluoro-7-nitrobenzofurazan (NBD-F)　　　　　　　　　　　　　　　
  Toshiki Ishikawa; Hiroyuki Imai; Maki Kawai-Yamada
  LIPIDS, Volume：49, Number：3, First page：295, Last page：304, Mar. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11745-013-3871-6
  DOI ID：10.1007/s11745-013-3871-6, ISSN：0024-4201, eISSN：1558-9307, ORCID：45646367, Web of Science ID：WOS:000332665600009
• Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata　　　　　　　　　　　　　　　
  Masayuki Kakimoto; Toshiki Ishikawa; Atsuko Miyagi; Kazuaki Saito; Motonobu Miyazaki; Takashi Asaeda; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  JOURNAL OF PLANT PHYSIOLOGY, Volume：171, Number：3-4, First page：292, Last page：300, Feb. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2013.09.005
  DOI ID：10.1016/j.jplph.2013.09.005, ISSN：0176-1617, ORCID：45646366, Web of Science ID：WOS:000332052200013
• Advanced LC-MS/MS techniques dissecting diverse isomers of plant sphingolipid species.　　　　　　　　　　　　　　　
  Ishikawa T; Yanagawa D; Kawai-Yamada M; Imai H
  J. Anal. Bioanal. Tech., Volume：S5, First page：7, 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.4172/2155-9872.S5-007
  DOI ID：10.4172/2155-9872.S5-007
• Plastidic protein Cdf1 is essential in Arabidopsis embryogenesis　　　　　　　　　　　　　　　
  Maki Kawai-Yamada; Minoru Nagano; Masayuki Kakimoto; Hirofumi Uchimiya
  PLANTA, Volume：239, Number：1, First page：39, Last page：46, Jan. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-013-1966-1
  DOI ID：10.1007/s00425-013-1966-1, ISSN：0032-0935, eISSN：1432-2048, ORCID：45646370, Web of Science ID：WOS:000329240700003
• Arabidopsis glycerol-3-phosphate permease 4 is localized in the plastids and involved in the accumulation of seed oil　　　　　　　　　　　　　　　
  Hiromitsu Kawai; Toshiki Ishikawa; Toshiaki Mitsui; Shin Kore-eda; Maki Kawai-Yamada; Jun-ichi Ohnishi
  PLANT BIOTECHNOLOGY, Volume：31, Number：2, First page：159, Last page：U80, 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.14.0222a
  DOI ID：10.5511/plantbiotechnology.14.0222a, ISSN：1342-4580, ORCID：45646365, Web of Science ID：WOS:000339501900009
• Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays　　　　　　　　　　　　　　　
  Sayaka Kitano; Atsuko Miyagi; Yutaka Oono; Yoshihiro Hase; Issay Narumi; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  Metabolomics, Volume：11, Number：1, First page：134, Last page：142, 2014, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1007/s11306-014-0684-4
  Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939895666&origin=inward
  Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84939895666&origin=inward
  DOI ID：10.1007/s11306-014-0684-4, ISSN：1573-3882, eISSN：1573-3890, ORCID：45646362, SCOPUS ID：84939895666, Web of Science ID：WOS:000348343300013
• Rapid Induction of Lipid Droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by Brefeldin A　　　　　　　　　　　　　　　
  Sangwoo Kim; Hanul Kim; Donghwi Ko; Yasuyo Yamaoka; Masumi Otsuru; Maki Kawai-Yamada; Toshiki Ishikawa; Hee-Mock Oh; Ikuo Nishida; Yonghua Li-Beisson; Youngsook Lee
  PLOS ONE, Volume：8, Number：12, Dec. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0081978
  DOI ID：10.1371/journal.pone.0081978, ISSN：1932-6203, ORCID：45646376, Web of Science ID：WOS:000328734200021
• Metabolome analysis of food-chain between plants and insects　　　　　　　　　　　　　　　
  Atsuko Miyagi; Maki Kawai-Yamada; Minori Uchimiya; Noriyuki Ojima; Koichi Suzuki; Hirofumi Uchimiya
  METABOLOMICS, Volume：9, Number：6, First page：1254, Last page：1261, Dec. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-013-0542-9
  DOI ID：10.1007/s11306-013-0542-9, ISSN：1573-3882, eISSN：1573-3890, ORCID：45646374, Web of Science ID：WOS:000326926700012
• Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp PCC 6803　　　　　　　　　　　　　　　
  Yuki Kaniya; Ayumi Kizawa; Atsuko Miyagi; Maki Kawai-Yamada; Hirofumi Uchimiya; Yasuko Kaneko; Yoshikata Nishiyama; Yukako Hihara
  PLANT PHYSIOLOGY, Volume：162, Number：2, First page：1153, Last page：1163, Jun. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.113.218784
  DOI ID：10.1104/pp.113.218784, ISSN：0032-0889, ORCID：45646372, Web of Science ID：WOS:000319819900047
• An antagonist treatment in combination with tracer experiments revealed isocitrate pathway dominant to oxalate biosynthesis in Rumex obtusifolius L.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Minori Uchimiya; Maki Kawai-Yamada; Hirofumi Uchimiya
  METABOLOMICS, Volume：9, Number：3, First page：590, Last page：598, Jun. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-012-0486-5
  DOI ID：10.1007/s11306-012-0486-5, ISSN：1573-3882, ORCID：45646371, Web of Science ID：WOS:000318905100007
• NAD⁺ accumulation as a metabolic off switch for orthodox pollen.　　　　　　　　　　　　　　　
  Hashida SN; Kawai-Yamada M; Uchimiya H
  Plant signaling & behavior, Volume：8, Number：5, First page：e23937, May 2013, [Reviewed]
  DOI：https://doi.org/10.4161/psb.23937
  DOI ID：10.4161/psb.23937, ISSN：1559-2316, PubMed ID：23428890
• Accelerated NAD metabolism reveals the essential role of NADH/NAD ratio on onset of senescence and carbon stock redistribution in Arabidopsis
  HASHIDA Shin‐nosuke; KITAZAKI Kazuyoshi; SHOJI Kazuhiro; GOTO Fumiyuki; YOSHIHARA Toshihiro; KAWAI‐YAMADA Maki; UCHIMIYA Hirofumi
  日本植物生理学会年会要旨集, Volume：54th, First page：244, Mar. 2013
  English
  J-Global ID：201302281097335366
• Impact of aluminium stress on oxalate and other metabolites in Rumex obtusifolius　　　　　　　　　　　　　　　
  A. Miyagi; M. Uchimiya; M. Kawai-Yamada; H. Uchimiya
  WEED RESEARCH, Volume：53, Number：1, First page：30, Last page：41, Feb. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-3180.2012.00949.x
  DOI ID：10.1111/j.1365-3180.2012.00949.x, ISSN：0043-1737, ORCID：45646373, Web of Science ID：WOS:000312996900004
• Effect of gamma ray irradiation on Rumex obtusifolius L.　　　　　　　　　　　　　　　
  Sayaka Kitano; Atsuko Miyagi; Yutaka Oono; Yoshihiro Hase; Issay Narumi; Hirofumi Uchimiya; Maki Kawai-Yamada
  First page：101, Jan. 2013
• The role of plant bax inhibitor-1 in suppressing H2O 2-induced cell death　　　　　　　　　　　　　　　
  Toshiki Ishikawa; Hirofumi Uchimiya; Maki Kawai-Yamada
  Methods in Enzymology, Volume：527, First page：239, Last page：256, 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/B978-0-12-405882-8.00013-1
  DOI ID：10.1016/B978-0-12-405882-8.00013-1, ISSN：0076-6879, ORCID：45646377, PubMed ID：23830635, SCOPUS ID：84879857652, Web of Science ID：WOS:000322846400014
• NAD(+) Accumulation during Pollen Maturation in Arabidopsis Regulating Onset of Germination　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Hideyuki Takahashi; Kentaro Takahara; Maki Kawai-Yamada; Kazuyoshi Kitazaki; Kazuhiro Shoji; Fumiyuki Goto; Toshihiro Yoshihara; Hirofumi Uchimiya
  MOLECULAR PLANT, Volume：6, Number：1, First page：216, Last page：225, Jan. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/mp/sss071
  DOI ID：10.1093/mp/sss071, ISSN：1674-2052, ORCID：45646375, Web of Science ID：WOS:000314117100022
• SMALL ACICIC PROTEIN1 Acts with RUB Modification Components, the COP9 Signalosome and AXR1 to Regulate Growth and Development of Arabidopsis (vol 160, pg 93, 2012)　　　　　　　　　　　　　　　
  A. Nakasone; M. Fujiwara; Y. Fukao; K. K. Biswas; A. Rahman; M. Kawai-Yamada; Narumi, I; H. Uchimiya; Y. Oono
  PLANT PHYSIOLOGY, Volume：160, Number：3, First page：1674, Last page：1674, Nov. 2012, [Reviewed]
  English
  DOI：https://doi.org/10.1104/pp.112.900448
  DOI ID：10.1104/pp.112.900448, ISSN：0032-0889, ORCID：45646378, Web of Science ID：WOS:000310584200039
• SMALL ACIDIC PROTEIN1 acts with RUB modification components, the COP9 signalosome, and AXR1 to regulate growth and development of Arabidopsis.　　　　　　　　　　　　　　　
  Akari Nakasone; Masayuki Fujiwara; Yoichiro Fukao; Kamal Kanti Biswas; Abidur Rahman; Maki Kawai-Yamada; Issay Narumi; Hirofumi Uchimiya; Yutaka Oono
  Plant physiology, Volume：160, Number：1, First page：93, Last page：105, Sep. 2012, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.111.188409
  DOI ID：10.1104/pp.111.188409, ORCID：45646379, PubMed ID：22576848, PubMed Central ID：PMC3440233, Web of Science ID：WOS:000308675100011
• Arabidopsis Sphingolipid Fatty Acid 2-Hydroxylases (AtFAH1 and AtFAH2) Are Functionally Differentiated in Fatty Acid 2-Hydroxylation and Stress Responses　　　　　　　　　　　　　　　
  Nagano, M; Takahara, K; Fujimoto, M; Tsutsumi, N; Uchimiya, H; Kawai-Yamada, M
  PLANT PHYSIOLOGY, Volume：159, Number：3, First page：1138, Last page：1148, Jul. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.112.199547
  DOI ID：10.1104/pp.112.199547, ISSN：0032-0889, eISSN：1532-2548, Web of Science ID：WOS:000305958000022
• Gsp1 triggers the sexual developmental program including inheritance of chloroplast DNA and mitochondrial DNA in Chlamydomonas reinhardtii.　　　　　　　　　　　　　　　
  Nishimura Y; Shikanai T; Nakamura S; Kawai-Yamada M; Uchimiya H
  The Plant cell, Volume：24, Number：6, First page：2401, Last page：2414, Jun. 2012, [Reviewed]
  DOI：https://doi.org/10.1105/tpc.112.097865
  DOI ID：10.1105/tpc.112.097865, ISSN：1040-4651, PubMed ID：22715041
• Nitrate addition alleviates ammonium toxicity without lessening ammonium accumulation, organic acid depletion and inorganic cation depletion in Arabidopsis thaliana shoots.　　　　　　　　　　　　　　　
  Hachiya T; Watanabe CK; Fujimoto M; Ishikawa T; Takahara K; Kawai-Yamada M; Uchimiya H; Uesono Y; Terashima I; Noguchi K
  Plant & cell physiology, Volume：53, Number：3, First page：577, Last page：591, Mar. 2012, [Reviewed]
  English
  DOI ID：10.1093/pcp/pcs012, ISSN：0032-0781, CiNii Articles ID：10030309550, CiNii Books ID：AA0077511X, PubMed ID：22318863, Web of Science ID：WOS:000301359500008
• Plant sphingolipid fatty acid 2-hydroxylases have unique characters unlike their animal and fungus counterparts　　　　　　　　　　　　　　　
  Minoru Nagano; Hirofumi Uchimiya; Maki Kawai-Yamada
  Plant Signaling and Behavior, Volume：7, Number：11, 2012, [Reviewed]
  Landes Bioscience, English, Scientific journal
  DOI：https://doi.org/10.4161/psb.21825
  DOI ID：10.4161/psb.21825, ISSN：1559-2324, PubMed ID：22918503, SCOPUS ID：84868513722
• Fate of C-13 in metabolic pathways and effects of high CO2 on the alteration of metabolites in Rumex obtusifolius L.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Kentaro Takahara; Ichiro Kasajima; Hideyuki Takahashi; Maki Kawai-Yamada; Hirofumi Uchimiya
  METABOLOMICS, Volume：7, Number：4, First page：524, Last page：535, Dec. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-010-0272-1
  DOI ID：10.1007/s11306-010-0272-1, ISSN：1573-3882, ORCID：45646382, Web of Science ID：WOS:000295991900006
• Morphological classification of plant cell deaths　　　　　　　　　　　　　　　
  W. G. van Doorn; E. P. Beers; J. L. Dangl; V. E. Franklin-Tong; P. Gallois; I. Hara-Nishimura; A. M. Jones; M. Kawai-Yamada; E. Lam; J. Mundy; L. A. J. Mur; M. Petersen; A. Smertenko; M. Taliansky; F. Van Breusegem; T. Wolpert; E. Woltering; B. Zhivotovsky; P. V. Bozhkov
  CELL DEATH AND DIFFERENTIATION, Volume：18, Number：8, First page：1241, Last page：1246, Aug. 2011, [Reviewed]
  English
  DOI：https://doi.org/10.1038/cdd.2011.36
  DOI ID：10.1038/cdd.2011.36, ISSN：1350-9047, eISSN：1476-5403, ORCID：45646384, Web of Science ID：WOS:000292634000002
• Molecular distinction in genetic regulation of nonphotochemical quenching in rice　　　　　　　　　　　　　　　
  Ichiro Kasajima; Kaworu Ebana; Toshio Yamamoto; Kentaro Takahara; Masahiro Yano; Maki Kawai-Yamada; Hirofumi Uchimiya
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：108, Number：33, First page：13835, Last page：13840, Aug. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1104809108
  DOI ID：10.1073/pnas.1104809108, ISSN：0027-8424, ORCID：45646383, Web of Science ID：WOS:000293895100091
• Bax inhibitor-1: a highly conserved endoplasmic reticulum-resident cell death suppressor　　　　　　　　　　　　　　　
  T. Ishikawa; N. Watanabe; M. Nagano; M. Kawai-Yamada; E. Lam
  CELL DEATH AND DIFFERENTIATION, Volume：18, Number：8, First page：1271, Last page：1278, Aug. 2011, [Reviewed]
  English
  DOI：https://doi.org/10.1038/cdd.2011.59
  DOI ID：10.1038/cdd.2011.59, ISSN：1350-9047, ORCID：45646380, Web of Science ID：WOS:000292634000006
• Characterization of Glucosylceramides in the Polygonaceae, Rumex obtusifolius L. Injurious Weed　　　　　　　　　　　　　　　
  Masayuki Watanabe; Atsuko Miyagi; Minoru Nagano; Maki Kawai-Yamada; Hiroyuki Imai
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：75, Number：5, First page：877, Last page：881, May 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.100802
  DOI ID：10.1271/bbb.100802, ISSN：0916-8451, eISSN：1347-6947, ORCID：45646381, Web of Science ID：WOS:000291519100010
• Roles of Plasma Membrane Microdomain in Cell Death Regulation Modulated by Bax Inhibitor-1　　　　　　　　　　　　　　　
  Ishikawa Toshiki; Aki Toshihiko; Yanagisawa Syuichi; Uchimiya Hirofumi; Kawai-Yamada Maki
  Plant and Cell Physiology Supplement, Volume：2011, First page：0404, Last page：0404, 2011
  Bax inhibitor-1 (BI-1) is an endoplasmic reticulum-localized cell death suppressor widely conserved in animals and plants. Our recent studies have revealed that Arabidopsis BI-1 (AtBI-1) interacts with sphingolipid-metabolic enzymes, leading us to further analyses to elucidate roles of BI-1-modulated sphingolipids on cell death regulation. Sphingolipids are well-known to play an important role for formation of liquid-ordered membrane microdomain in plants as well as animals. Here we show compositional alterations of plasma membrane microdomain in AtBI-1 overexpressing rice cells. Glycosphingolipid composition in plasma membrane microdomain was elevated in AtBI-1 overexpressor. Furthermore, shotgun proteomics combined with label-free comparative analysis revealed significant decreases of microdomain-localized proteins possible to participate in cell death regulation, such as hypersensitive cell death-associated proteins and scaffolds for protein complexes in microdomain. Based on these results, we will discuss functional roles of microdomain remodeling in the cell death regulatory mechanism of BI-1.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2011.0.0404.0
  DOI ID：10.14841/jspp.2011.0.0404.0, CiNii Articles ID：130006997013
• Metabolome analysis of oxalate accumulation in Rumex obtusifolius L. using ¹³CO₂ and high CO₂　　　　　　　　　　　　　　　
  Miyagi Atsuko; Kawai-Yamada Maki; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：2011, First page：0657, Last page：0657, 2011
  Rumex obtusifolius L. (perennial weed; Polygonaceae) is one of the oxalate-rich plants. Nevertheless, the mechanism of oxalate accumulation in plants is still unclear. Recent metabolome analyses of R. obtusifolius revealed that oxalate contents were highly correlated to citrate and ascorbate (Miyagi et al, Metabolomics, 2010a, b). We reported that oxalate content in leaves was affected by carbon source (such as citrate) in stems. Using CE-MS, we analyzed the carbon effects on oxalate accumulation and other metabolite levels in leaves and stems of R. obtusifolius. The experiment using ¹³CO₂ showed that ¹³C-oxalate was highly accumulated in leaves and that ¹³C mobilization from stems to "new leaves" was observed. High CO₂ (1000 ppm) experiment showed that oxalate content was increased by high CO₂ with Hoagland's solution, although oxalate levels was not changed by CO₂ alone.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2011.0.0657.0
  DOI ID：10.14841/jspp.2011.0.0657.0, CiNii Articles ID：130006995798
• A shotgun proteomics-based approach to identify membrane microdomain proteins involved in regulation of stress-induced cell death　　　　　　　　　　　　　　　
  Ishikawa Toshiki; Aki Toshihiko; Yanagisawa Shuichi; Nagano Minoru; Uchimiya Hirofumi; Kawai-Yamada Maki
  Abstracts for Annual Meeting of Japanese Proteomics Society, Volume：2011, First page：215, Last page：215, 2011
  Japanese Proteomics Society (Japan Human Proteome Organisation), Japanese
  DOI：https://doi.org/10.14889/jhupo.2011.0.215.0
  DOI ID：10.14889/jhupo.2011.0.215.0, CiNii Articles ID：130005454359
• Targeted metabolomics in an intrusive weed, Rumex obtusifolius L., grown under different environmental conditions reveals alterations of organ related metabolite pathway　　　　　　　　　　　　　　　
  Atsuko Miyagi; Kentaro Takahara; Hideyuki Takahashi; Maki Kawai-Yamada; Hirofumi Uchimiya
  METABOLOMICS, Volume：6, Number：4, First page：497, Last page：510, Dec. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-010-0220-0
  DOI ID：10.1007/s11306-010-0220-0, ISSN：1573-3882, ORCID：45646391, Web of Science ID：WOS:000283944300004
• Ammonium-dependent respiratory increase is dependent on the cytochrome pathway in Arabidopsis thaliana shoots　　　　　　　　　　　　　　　
  Takushi Hachiya; Chihiro K. Watanabe; Carolina Boom; Danny Tholen; Kentaro Takahara; Maki Kawai-Yamada; Hirofumi Uchimiya; Yukifumi Uesono; Ichiro Terashima; Ko Noguchi
  PLANT CELL AND ENVIRONMENT, Volume：33, Number：11, First page：1888, Last page：1897, Nov. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-3040.2010.02189.x
  DOI ID：10.1111/j.1365-3040.2010.02189.x, ISSN：0140-7791, ORCID：45646385, Web of Science ID：WOS:000283375200009
• Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Taketo Itami; Hideyuki Takahashi; Kentaro Takahara; Minoru Nagano; Maki Kawai-Yamada; Kazuhiro Shoji; Fumiyuki Goto; Toshihiro Yoshihara; Hirofumi Uchimiya
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：61, Number：13, First page：3813, Last page：3825, Aug. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erq190
  DOI ID：10.1093/jxb/erq190, ISSN：0022-0957, ORCID：45646389, Web of Science ID：WOS:000280905400028
• Effects of AOX1a Deficiency on Plant Growth, Gene Expression of Respiratory Components and Metabolic Profile Under Low-Nitrogen Stress in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Chihiro K. Watanabe; Takushi Hachiya; Kentaro Takahara; Maki Kawai-Yamada; Hirofumi Uchimiya; Yukifumi Uesono; Ichiro Terashima; Ko Noguchi
  PLANT AND CELL PHYSIOLOGY, Volume：51, Number：5, First page：810, Last page：822, May 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcq033
  DOI ID：10.1093/pcp/pcq033, ISSN：0032-0781, eISSN：1471-9053, ORCID：45646386, Web of Science ID：WOS:000277734900012
• Metabolome and Photochemical Analysis of Rice Plants Overexpressing Arabidopsis NAD Kinase Gene　　　　　　　　　　　　　　　
  Kentaro Takahara; Ichiro Kasajima; Hideyuki Takahashi; Shin-nosuke Hashida; Taketo Itami; Haruko Onodera; Seiichi Toki; Shuichi Yanagisawa; Maki Kawai-Yamada; Hirofumi Uchimiya
  PLANT PHYSIOLOGY, Volume：152, Number：4, First page：1863, Last page：1873, Apr. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.110.153098
  DOI ID：10.1104/pp.110.153098, ISSN：0032-0889, eISSN：1532-2548, ORCID：45646388, Web of Science ID：WOS:000276335900011
• Principal component and hierarchical clustering analysis of metabolites in destructive weeds; polygonaceous plants　　　　　　　　　　　　　　　
  Atsuko Miyagi; Hideyuki Takahashi; Kentaro Takahara; Takayuki Hirabayashi; Yoshiki Nishimura; Takafumi Tezuka; Maki Kawai-Yamada; Hirofumi Uchimiya
  METABOLOMICS, Volume：6, Number：1, First page：146, Last page：155, Mar. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11306-009-0186-y
  DOI ID：10.1007/s11306-009-0186-y, ISSN：1573-3882, ORCID：45646390, Web of Science ID：WOS:000275444800013
• The relationship between cell death suppressor (AtBI-1) and metabolism of sphingolipid fatty acid in Arabidopsis　　　　　　　　　　　　　　　
  Nagano Minoru; Kakuta Chikako; Uchimiya Hirofumi; Kawai-Yamada Maki
  Plant and Cell Physiology Supplement, Volume：2010, First page：0545, Last page：0545, 2010
  Programmed cell death is crucial for developments and responses to various stresses in plants, and is highly regulated by a wide variety of factors. Bax Inhibitor-1 (BI-1) is a widely conserved cell-death suppressor. Arabidopsis BI-1 (AtBI-1) is a 7-times transmembrane protein localized in endoplasmic reticulum (ER), and suppresses cell death induced by oxidative stress, which generates reactive oxygen species (ROS) in plant cells. In addition, calmodulin interacts with the C-terminus of AtBI-1, which is essential for its function. However, the molecular mechanism of BI-1-mediated cell death is still unclear.
  We suggested that AtBI-1 interacts with sphingolipid fatty acid metabolic enzymes via an electron transfer protein, cytochrome b₅. Sphingolipid has a 2-hydroxylated very-long-chain fatty acid (2-hydroxy VLCFA), and it is implied that AtBI-1 interacts with sphingolipid fatty acid 2-hydroxylases (AtFAH) and VLCFA condensing enzymes (AtELO). Here we show the relationship between AtBI-1 and sphingolipid fatty acid generated by AtFAH and AtELO.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2010.0.0545.0
  DOI ID：10.14841/jspp.2010.0.0545.0, CiNii Articles ID：130006991529
• Programmed cell death in plants　　　　　　　　　　　　　　　
  Avijit Das; Maki Kawai-Yamada; Hirofumi Uchimiya
  Abiotic Stress Adaptation in Plants: Physiological, Molecular and Genomic Foundation, First page：371, Last page：383, 2010, [Reviewed]
  Springer Netherlands, English, In book
  DOI：https://doi.org/10.1007/978-90-481-3112-9_17
  DOI ID：10.1007/978-90-481-3112-9_17, SCOPUS ID：84856505544
• Metabolome Analysis of Response to Oxidative Stress in Rice Suspension Cells Overexpressing Cell Death Suppressor Bax Inhibitor-1　　　　　　　　　　　　　　　
  Toshiki Ishikawa; Kentaro Takahara; Takayuki Hirabayashi; Hideo Matsumura; Shizuko Fujisawa; Ryohei Terauchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANT AND CELL PHYSIOLOGY, Volume：51, Number：1, First page：9, Last page：20, Jan. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcp162
  DOI ID：10.1093/pcp/pcp162, ISSN：0032-0781, eISSN：1471-9053, ORCID：45646387, Web of Science ID：WOS:000273704500002
• SMAP2 protein potentially medicates the response to synthetic auxin, 2,4-D, in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Akari Nakasone; Maki Kawai-Yamada; Tomohiro Kiyosue; Issay Narumi; Hirofumi Uchimiya; Yutaka Oono
  First page：65, Dec. 2009
• A gene encoding SMALL ACIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  Akari Nakasone; Maki Kawai-Yamada; Tomohiro Kiyosue; Issay Narumi; Hirofumi Uchimiya; Yutaka Oono
  Journal of plant physiology, Volume：166, Number：12, First page：1307, Last page：13, Aug. 2009, [Reviewed], ［International magazine］
  English
  DOI：https://doi.org/10.1016/j.jplph.2009.02.005
  DOI ID：10.1016/j.jplph.2009.02.005, ORCID：45646392, PubMed ID：19307045, Web of Science ID：WOS:000268818200008
• Metabolite analysis of alien weed Rumex obtusifolius L.　　　　　　　　　　　　　　　
  Miyagi Atsuko; Hirabayashi Takayuki; Takahashi Hideyuki; Nishimura Yoshiki; Takahara Kentaro; Kawai-Yamada Maki; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：2009, First page：0315, Last page：0315, 2009
  Rumex obtusifolius L. (Polygonaceae) is one of the most formidable weeds in infesting fields, arable lands and pastures through the world. In the early 20 century, the occurrence of R. obtusifolius in Japan was first reported in the northern island, whereas today it can be found in almost all areas.
  Rumex plants contain high concentrations of oxalate. Excess uptake of this substance is toxic to animals. In plants, oxalate plays an important role as a source of hydrogen peroxide, which may act as defense molecules. Furthermore, some plants secrete oxalate to the rhizosphere, which could detoxify metallic ions.
  In this investigation, we analyzed the oxalate metabolism in R. obtusifolius. Metabolite analysis by CE-MS showed that higher level of oxalate and amino acids were accumulated in R. obtusifolius. The analysis of R. obtusifolius grown in the dark indicated that citrate in stems play an important role in oxalate accumulation in leaves.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2009.0.0315.0
  DOI ID：10.14841/jspp.2009.0.0315.0, CiNii Articles ID：130006994320
• The Relationship Between NAD Biosynthesis and Stomatal Closure　　　　　　　　　　　　　　　
  Hashida Shin-nosuke; Takahashi Hideyuki; Takahara Kentaro; Nagano Minoru; Kawai-Yamada Maki; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：2009, First page：0325, Last page：0325, 2009
  Recent research has underscored the importance of nicotinamide adenine dinucleotide (NAD) biosynthesis. In Arabidopsis, genes involved in the NAD biosynthetic pathway have not been fully determined. Previously, we identified the gene encoding NAD synthetase (NADS), which acts in the final step of NAD biosynthesis. Transcriptional analysis revealed that guard cells (GCs) have relatively high levels of NAD biosynthetic gene expression. The NAD level in the epidermis including GCs was transiently decreased by abscisic acid (ABA), whereas nicotinate mononucleotide adenyltransferase (NMNAT) activity was increase by ABA; however, the increase was significantly delayed compared to the decrease in NAD. The activity of NADS was in a steady state during NAD fluctuation. Stomatal movement and drought tolerance were correlated to the activity of NMNAT. We proposed that NAD biosynthetic activity is involved in regulation of stomatal aperture, possibly via the regulation of NMNAT activity.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2009.0.0325.0
  DOI ID：10.14841/jspp.2009.0.0325.0, CiNii Articles ID：130006994190
• Nicotinamide nucleotides enhance plant metabolic processes　　　　　　　　　　　　　　　
  Takahashi Hideyuki; Takahara Kentaro; Hashida Shin-nosuke; Kawai-Yamada Maki
  Plant and Cell Physiology Supplement, Volume：2009, First page：0174, Last page：0174, 2009
  Nicotinamide nucleotides (NAD and NADP) are important co-factors in various metabolic processes in living organisms. Although both NAD and NADP are redox regulators of living cells, these compounds likely play overlapping but different roles in other biological processes. NAD and NADP are synthesized in NAD biosynthetic pathway comprising NADS, NMNAT, and NADK. The levels of NAD and NADP appear to be regulated by these enzymes. In this study, we analyzed transgenic Arabidopsis plants, which have several levels of NAD and NADP to elucidate the regulating mechanisms of these co-factors. Our results showed that the decrease of NADP level inhibited photosynthetic electron transport. Furthermore, fluctuation of NAD and NADP levels affected several metabolite processes, indicating that NAD and NADP are key factors that control plant metabolism and also suggest the possibility that enhancement of co-factor synthesis is a workable strategy for improving performances of plant.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2009.0.0174.0
  DOI ID：10.14841/jspp.2009.0.0174.0, CiNii Articles ID：130006991067
• The roles of sphingolipid fatty acid hydroxylase in Arabidopsis　　　　　　　　　　　　　　　
  Nagano Minoru; Uchimiya Hirofumi; Kawai-Yamada Maki
  Plant and Cell Physiology Supplement, Volume：2009, First page：0216, Last page：0216, 2009
  Sphingolipids are a large class of lipids ubiquitously present in eukaryotic cell membranes and essential for various cellular reactions such as signal transduction, protein transport and programmed cell death. The structural diversity of sphingolipids derives from more than 300 distinct head groups as well as various modifications on hydrocarbon chains of the hydrophobic ceramide moiety. One of the main modifications of ceramide structure is 2-hydroxylation of the fatty acids. The 2-hydroxylation of the fatty acids is catalyzed by fatty acid hydroxylase (FAH). Two Arabidopsis FAHs (AtFAH1 and AtFAH2) are localized in endoplasmic reticulum (ER) membrane. In addition, AtFAHs were activated by ER-localized cytochrome b5 through their direct interaction. Here we show the functions of AtFAHs and the roles of 2-hydroxy fatty acids in Arabidopsis.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2009.0.0216.0
  DOI ID：10.14841/jspp.2009.0.0216.0, CiNii Articles ID：130006991164
• Chloroplast NAD kinase Enhances Carbon and Nitrogen Metabolism　　　　　　　　　　　　　　　
  Takahashi Hideyuki; Hashida Shin-nosuke; Fujimori Tamaki; Kawai-Yamada Maki; Tamoi Masahiro; Shigeoka Shigeru; Yanagisawa Shuichi; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：2008, First page：0297, Last page：0297, 2008
  Chloroplast NAD kinase enhances carbon and nitrogen metabolism
  Chloroplast NAD kinase (NADK2) has been identified as an essential enzyme for chlorophyll synthesis, photosynthetic electron transport, and xanthophyll cycle. Metabolite analysis revealed that the contents of intermediates of the Calvin cycle were increased in transgenic plants overexpressing NADK2 gene (NADK2-OX). The activities of RubisCO and glyceraldehyde-3-phosphate dehydrogenase as well as CO₂ uptake were also increased, indicating that carbon fixation was enhanced in NADK2-OX. On the other hand, the accumulation of free amino acids, especially glutamine and glutamate were observed in NADK2-OX. Furthermore, the expression levels of chloroplast glutamine synthetase and NADH-dependent glutamate synthase genes were increased. The uptake and reduction process of nitrate were also increased. It therefore seemed that the activation of GS/GOGAT pathway and nitrate reduction contributed to amino acid overproduction. These results indicate that NADK2 overexpression enhances both carbon and nitrate metabolism.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2008.0.0297.0
  DOI ID：10.14841/jspp.2008.0.0297.0, CiNii Articles ID：130006994378
• Analysis of AtBI-1-mediated cell death suppression through sphingolipid-related enzymes　　　　　　　　　　　　　　　
  Nagano Minoru; Ihara-Ohori Yuri; Kakuta Chikako; Uchimiya Hirofumi; Kawai-Yamada Maki
  Plant and Cell Physiology Supplement, Volume：2008, First page：0279, Last page：0279, 2008
  In higher plants, programmed cell death is highly regulated by various factors. Bax Inhibitor-1 (BI-1) is a widely conserved cell death suppressor. Arabidopsis BI-1 (AtBI-1) is approximately 26kDa protein with 7 transmembrane domains and localizes in ER. Overexpression of AtBI-1 suppresses H₂O₂-. SA- and elicitor-induced cell death in plant cells. Moreover, AtBI-1 possesses coiled-coil structure in the C-terminus, which is essential in AtBI-1 function and interacts with calmodulin. However, the mechanism of AtBI-1-mediated cell death suppression has not yet been elucidated.
  Recently, we revealed that AtBI-1 interacted with AtFAH (Arabidopsis fatty acid hydroxylase) via cytochrome b₅ (Cb5). Cb5 is an electron transfer protein, which is related to fatty acid synthesis, and AtFAH is a hydroxylase of sphingolipid fatty acid localizing in ER. Lately, the relationships between sphingolipids and cell death have been discussed vigorously. AtBI-1 may suppress cell death through sphingolipid regulation.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2008.0.0279.0
  DOI ID：10.14841/jspp.2008.0.0279.0, CiNii Articles ID：130006993957
• Pollen Dormancy in Nicotinate/nicotinamide Mononucleotide Adenyltransferase Mutant　　　　　　　　　　　　　　　
  Hashida Shin-nosuke; Takahara Kentaro; Takahashi Hideyuki; Kawai-Yamada Maki; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：2008, First page：0238, Last page：0238, 2008
  Nicotinamide adenine dinucleotide (NAD) coenzyme is abundant in pollen grain compared to the other inflorescent tissues of Arabidopsis. Pollen lacking a key NAD biosynthetic gene, nicotinate/nicotinamide mononucleotide adenyltransferase (NMNAT), was deficient in tube growth and dormancy. Tracer experiment revealed that the nmnat pollen incompletely eliminated the HPTS-containing moisture, suggesting that nmnat pollen was un-dormant. Under high humidity environment, nmnat/+ plants had long pollen tubes inside the anthers, namely precocious pollen tube growth. Even under dehydrated condition, round pollen with bulges can be distinguished in pollen population of nmnat/+ plants. Un-dormant pollen generally remains to be metabolically active, leading to senescence acceleration. In fact, the longevity of nmnat pollen was quite shorter than that of wild type. Thus, the NAD biosynthesis is essential for acquisition of drought tolerance and transition to pollen dormancy.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2008.0.0238.0
  DOI ID：10.14841/jspp.2008.0.0238.0, CiNii Articles ID：130006993793
• Identification of novel mitochondrial membrane protein (Cdf 3) from Arabidopsis thaliana and its functional analysis in a yeast system　　　　　　　　　　　　　　　
  Kyung-Min Kim; Do-Youn Jun; Sang-Kook Kim; Chang-Kil Kim; Byung Oh Kim; Young-Ho Kim; Wan Park; Jae-Keun Sohn; Aiko Hirata; Maki Kawai-Yamada; Hirofumi Uchimiya; Dai-Hee Kim; Ill-Whan Sul
  JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, Volume：17, Number：6, First page：891, Last page：896, Jun. 2007, [Reviewed]
  English, Scientific journal
  ISSN：1017-7825, eISSN：1738-8872, Web of Science ID：WOS:000247703500002
• Young Investigator Award - Plant cell death as an oxidative stress response　　　　　　　　　　　　　　　
  Maki Kawai-Yamada
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S16, Last page：S16, 2007, [Reviewed]
  English
  DOI：https://doi.org/10.14841/jspp.2007.0.A002.0
  DOI ID：10.14841/jspp.2007.0.A002.0, ISSN：0032-0781, ORCID：45646410, Web of Science ID：WOS:000245922700060
• Mechanism of metabolic activation through altered NAD metabolism
  Maki Kawai-Yamada; Hideyuki Takahashi; Shin-Nosuke Hashida; Hirofumi Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S9, Last page：S9, 2007, [Reviewed]
  English
  ISSN：0032-0781, ORCID：45646407, Web of Science ID：WOS:000245922700036
• Functional analysis of Arabidopsis thaliana NAD biosynthetic genes　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Taketo Itami; Hideyuki Takahashi; Maki Kawai-Yamada; Hirofumi Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S42, Last page：S42, 2007, [Reviewed]
  English
  DOI：https://doi.org/10.14841/jspp.2007.0.104.0
  DOI ID：10.14841/jspp.2007.0.104.0, ISSN：0032-0781, ORCID：45646406, Web of Science ID：WOS:000245922700166
• The mitochondrial fission regulator DRP3B does not regulate cell death in plants　　　　　　　　　　　　　　　
  Keiko Yoshinaga; Masaru Fujimoto; Shin-Ichi Arimura; Nobuhiro Tsutsumi; Hirofumi Uchimiya; Maki Kawai-Yamada
  Handbook of Environmental Chemistry, Volume 5: Water Pollution, Volume：97, Number：6, First page：1145, Last page：1149, Jun. 2006, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/aob/mcl057
  DOI ID：10.1093/aob/mcl057, ISSN：1433-6863, SCOPUS ID：33947317551
• Analysis of the Arabidopsis thaliana cell growth defect factor 2 (Cdf2) suppressing yeast cell proliferation　　　　　　　　　　　　　　　
  Kyung-Min Kim; Yong Suk Lim; Ill Whan Sul; Aiko Hirata; Maki Kawai-Yamada; Hirofumi Uchimiya
  KOREAN JOURNAL OF GENETICS, Volume：28, Number：2, First page：201, Last page：206, Jun. 2006, [Reviewed]
  English, Scientific journal
  ISSN：0254-5934, Web of Science ID：WOS:000239182700014
• NMNAT, a key enzyme in NAD biosynthesis, required for normal pollen tube elongation in Arabidopsis　　　　　　　　　　　　　　　
  SN Hashida; H Takahashi; M Kawai-Yamada; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S148, Last page：S148, 2006, [Reviewed]
  English
  DOI：https://doi.org/10.14841/jspp.2006.0.495.0
  DOI ID：10.14841/jspp.2006.0.495.0, ISSN：0032-0781, ORCID：45646417, Web of Science ID：WOS:000236401401077
• Metabolome analysis of rice cells treated with the cell wall extracts of Magnaporthe grisea
  H Takahashi; H Matsumura; M Kawai-Yamada; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S83, Last page：S83, 2006, [Reviewed]
  English
  ISSN：0032-0781, ORCID：45646416, Web of Science ID：WOS:000236401400329
• Metabolic studies of oxalate rich plants
  A Miyagi; H Takahashi; M Kawai-Yamada; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S60, Last page：S60, 2006, [Reviewed]
  English
  ISSN：0032-0781, ORCID：45646415, Web of Science ID：WOS:000236401400240
• Analysis of proteins interacting with cell death suppressor (AtBI-1)
  M Nagano; Y Ihara-Ohori; K Yoshinaga; H Uchimiya; M Kawai-Yamada
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S43, Last page：S43, 2006, [Reviewed]
  English
  ISSN：0032-0781, ORCID：45646412, Web of Science ID：WOS:000236401400171
• The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm　　　　　　　　　　　　　　　
  H Fukuzawa; LH Yu; C Umeda-Hara; M Tagawa; H Uchimiya
  PLANT CELL REPORTS, Volume：23, Number：4, First page：231, Last page：235, Oct. 2004, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00299-004-0813-z
  DOI ID：10.1007/s00299-004-0813-z, ISSN：0721-7714, Web of Science ID：WOS:000224614500008
• Isolation of a cotton CAP gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation
  M Kawai; S Aotsuka; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：39, Number：12, First page：1380, Last page：1383, Dec. 1998, [Reviewed]
  English, Scientific journal
  ISSN：0032-0781, ORCID：45646432, Web of Science ID：WOS:000077761600019
• Dissection of cell cycle and cell death in rice root.
  内宮博文; 川合真紀; 梅田(原)千景; 梅田正明
  育種学雑誌, Volume：48, First page：32, Apr. 1998
  Japanese
  ISSN：0536-3683, J-Global ID：200902180414092543
• Mechanism of aerenchyma formation by cell death.
  川合真紀; 梅田正明; 内宮博文
  月刊組織培養, Volume：22, Number：1, First page：8, Last page：12, Jan. 1996
  Japanese
  ISSN：0386-1791, J-Global ID：200902141757145191
• TISSUE-SPECIFIC LOCALIZATION OF ADENYLATE KINASE IN RICE (ORYZA-SATIVA L) PLANTS
  M KAWAI; H UCHIMIYA
  JOURNAL OF PLANT PHYSIOLOGY, Volume：146, Number：3, First page：239, Last page：242, Jun. 1995, [Reviewed]
  English, Scientific journal
  ISSN：0176-1617, ORCID：45646436, Web of Science ID：WOS:A1995RJ37600008
• Strcuture and stress response of rice adenylate kinase(AK) genes.
  川合真紀; 梅田正明; 内宮博文
  育種学雑誌, Volume：44, First page：253, 1994
  Japanese
  ISSN：0536-3683, J-Global ID：200902144795110961
• Structure and expression of rice nucleotide kinase (AK and NDK) genes.
  川合真紀; 矢野明; 梅田正明; 内宮博文
  Jpn J Breed, Volume：43, First page：39, 1993
  Japanese
  ISSN：0536-3683, J-Global ID：200902124807785403
• MOLECULAR CHARACTERIZATION OF CDNA-ENCODING FOR ADENYLATE KINASE OF RICE (ORYZA-SATIVA L)　　　　　　　　　　　　　　　
  M KAWAI; S KIDOU; A KATO; H UCHIMIYA
  PLANT JOURNAL, Volume：2, Number：6, First page：845, Last page：854, Nov. 1992, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1046/j.1365-313X.1992.t01-1-00999.x
  DOI ID：10.1046/j.1365-313X.1992.t01-1-00999.x, ISSN：0960-7412, ORCID：45646437, Web of Science ID：WOS:A1992JY25100002

• Analyses of transcriptional regulation of cuticular wax accumulation in response to dehydration　　　　　　　　　　　　　　　
  浦野薫; 圓山恭之進; 大島良美; 大島良美; 坂本真吾; 石川寿樹; 川合真紀; 佐藤繭子; 豊岡公徳; 篠崎和子; 篠崎和子; 篠崎一雄
  日本植物生理学会年会(Web), Volume：62nd, 2021
  J-Global ID：202102212550428101
• イネ品種間におけるシュウ酸蓄積の比較解析　　　　　　　　　　　　　　　
  宮城敦子; 安達俊輔; 大川泰一郎; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：37th, First page：68, 25 Aug. 2019
  Japanese
  J-Global ID：201902223570072576
• 光合成誘導反応のイネ品種間差に関わる生理的要因の解析　　　　　　　　　　　　　　　
  安達俊輔; 田中佑; 宮城敦子; 鹿島誠; 手塚あゆみ; 戸谷吉博; 小林俊造; 大久保智司; 清水浩; 川合真紀; 永野惇; 矢守航
  日本作物学会講演会要旨集, Volume：247th, First page：65, 28 Mar. 2019
  Japanese
  J-Global ID：201902277546415649
• 光合成における電子受容体NADP⁺供給システムと電子伝達の交互作用　　　　　　　　　　　　　　　
  橋田慎之介; 川合真紀
  Volume：60th, 2019
  J-Global ID：201902254199254282
• シロイヌナズナVND遺伝子群の遺伝学的解析
  伊藤敦也; 久保稔; 大谷美沙都; 石川寿樹; 川合真紀; 出村拓; 山口雅利
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：36th, First page：153, 15 Aug. 2018
  Japanese
  J-Global ID：201802264094945466
• イネにおける染色体部分置換がシュウ酸蓄積に及ぼす影響
  宮城敦子; 安達俊輔; 野口航; 常田岳志; 臼井靖浩; 中村浩史; 酒井英光; 長谷川利拡; 山本敏夫; 大川泰一郎; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：36th, First page：115, 15 Aug. 2018
  Japanese
  J-Global ID：201802258186947949
• イオンビーム照射イネ系統におけるメタボローム解析
  宮城敦子; 西丸拓也; 大野豊; 長谷純宏; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：34th, First page：77, 20 Aug. 2016
  Japanese
  J-Global ID：201602249993963032
• イオンビームを照射した高シュウ酸植物エゾノギシギシの代謝解析
  宮城敦子; 北野沙也佳; 長谷純宏; 大野豊; 山口雅利; 川合真紀
  日本植物学会大会研究発表記録, Volume：79th, First page：134, 01 Sep. 2015
  Japanese
  J-Global ID：201502214850620962
• NAD(P)代謝改変シロイヌナズナの作出および代謝解析　　　　　　　　　　　　　　　
  鈴木渉太; 宮城敦子; 石川寿樹; 刑部敬史; 長野稔; 山口雅利; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：33rd, First page：140, 20 Jul. 2015
  Japanese
  J-Global ID：201502202919376033
• Metabolome analysis of leaves of Rumex obtusifolius L. irradiated with ion beams.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Sayaka Kitano; Yutaka Oono; Yoshihiro Hase; Maki Kawai-Yamada
  JAEA Review 2014-050, First page：114, Mar. 2015
  Japanese
• 放射線照射高シュウ酸植物のメタボローム解析
  宮城敦子; 北野沙也佳; 大野豊; 長谷純宏; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：32nd, First page：100, 30 Jul. 2014
  Japanese
  J-Global ID：201402237207551572
• Effects of gamma-ray irradiation on oxalate Metabolism in Rumex obtusifolius L.　　　　　　　　　　　　　　　
  Sayaka Kitano; Atsuko Miyagi; Yutaka Ohno; Yoshihiro Hase; Issay Narumi; Hirofumi Uchimiya; Maki Kawai-Yamada
  JAEA-Review, Volume：2013-059, First page：108, Mar. 2014
• 植物のアルミニウムストレスと代謝物解析
  Atsuko Miyagi; Maki Kawai-Yamada; Hirofumi Uchimiya
  農業および園芸, Volume：89, Number：1, First page：34, Last page：39, 01 Jan. 2014
  Japanese, Introduction commerce magazine
  ISSN：0369-5247, J-Global ID：201402268976322988
• ガンマ線が高シュウ酸植物の代謝に及ぼす影響
  北野沙也佳; 宮城敦子; 大野豊; 長谷純弘; 鳴海一成; 山口雅利; 内宮博文; 川合真紀
  日本植物学会大会研究発表記録, Volume：77th, First page：185, 20 Aug. 2013
  Japanese
  J-Global ID：201302255221317192
• 雑草のメタボローム研究〔3〕—エゾノギシギシの二酸化炭素応答—　　　　　　　　　　　　　　　
  宮城敦子; 川合真紀; 内宮博文
  農業および園芸, Volume：87, Number：7, First page：694-700, Jul. 2012
  Japanese
  ISSN：0369-5247, J-Global ID：201202276171386520
• Theories in PAM chlorophyll fluorescence measurement of plant leaves　　　　　　　　　　　　　　　
  Ichiro Kasajima; Maki Kawai-Yamada; Maki Kawai-Yamada; Hirofumi Uchimiya; Hirofumi Uchimiya
  Photochemistry: UV/VIS Spectroscopy, Photochemical Reactions and Photosynthesis, First page：351, Last page：371, 01 Dec. 2011
  SCOPUS ID：84892840962
• 雑草のメタボローム研究〔2〕—環境ストレス耐性とバイオマスエネルギー素材—　　　　　　　　　　　　　　　
  宮城敦子; 川合真紀; 内宮博文
  農業および園芸, Volume：86, Number：11, First page：1153-1159, Nov. 2011
  Japanese
  ISSN：0369-5247, J-Global ID：201102253283160276
• 雑草のメタボローム研究〔1〕—タデ科植物種間比較解析—　　　　　　　　　　　　　　　
  宮城敦子; 川合真紀; 内宮博文
  農業および園芸, Volume：86, Number：10, First page：1062-1066, Oct. 2011
  Japanese
  ISSN：0369-5247, J-Global ID：201102266438000976
• Promotion of Ageing in Transgenic Arabidopsis Plants Overexpressing Nicotinamide Adenine Dinucleotide Synthetase　　　　　　　　　　　　　　　
  Hashida Shin-nosuke; Takahara Kentaro; Johkan Masafumi; Shoji Kazuhiro; Goto Fumiyuki; Yoshihara Toshihiro; Kawai-Yamada Maki; Uchimiya Hirofumi
  Plant and Cell Physiology Supplement, Volume：52nd, First page：742, Last page：742, 2011
  Modulation of cellular energy homeostasis is an attractive alternative to improve crop yield and plant performance under stressful conditions. Previously, we reported that overexpression of Arabidopsis NAD kinase2, which produces chloroplastic NADP, promoted nitrogen assimilation in Arabidopsis. Consequently, metabolites associated with the Calvin cycle were also accumulated. In this study, we generated transgenic plants overexpressing Arabidopsis NAD synthetase gene (NADS). NADS overexpressor plants did not show any remarkable changes in vegetative growth and the level of NAD(P)(H) was unexpectedly unchanged albeit enhanced NADS activity. However, NADS overexpression caused early ageing coincidentally with bolting. Then, the levels of NAD⁺ was drastically decreased and the levels of intermediates of NAD biosynthesis (NaMN and NaAD) and NAD derivatives (nicotinate and nicotinamide) were dramatically increased in NADS overexpressors after bolting. These results suggest that the levels of NAD(P)(H) could be kept properly during vegetative phase even under NADS overexpression and that bolting may act as a signal for the alteration of NAD homeostasis.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2011.0.0742.0
  DOI ID：10.14841/jspp.2011.0.0742.0, J-Global ID：201102296779639831, CiNii Articles ID：130006998144
• Loss of Calmodulin Binding to Bax Inhibitor-1 Affects Pseudomonas-mediated Hypersensitive Response-associated Cell Death in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Maki Kawai-Yamada; Zenta Hori; Taro Ogawa; Yuri Ihara-Ohori; Katsunori Tamura; Minoru Nagano; Toshiki Ishikawa; Hirofumi Uchimiya
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：284, Number：41, First page：27998, Last page：28003, Oct. 2009
  English
  DOI：https://doi.org/10.1074/jbc.M109.037234
  DOI ID：10.1074/jbc.M109.037234, ISSN：0021-9258, CiNii Articles ID：80020605891, PubMed ID：19674971, Web of Science ID：WOS:000270676300028
• Estimation of the Relative Sizes of Rate Constants for Chlorophyll De-excitation Processes Through Comparison of Inverse Fluorescence Intensities　　　　　　　　　　　　　　　
  Ichiro Kasajima; Kentaro Takahara; Maki Kawai-Yamada; Hirofumi Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：50, Number：9, First page：1600, Last page：1616, Sep. 2009
  English
  DOI：https://doi.org/10.1093/pcp/pcp102
  DOI ID：10.1093/pcp/pcp102, ISSN：0032-0781, CiNii Articles ID：10027343022, PubMed ID：19602498, Web of Science ID：WOS:000270547300003
• Pleiotropic Modulation of Carbon and Nitrogen Metabolism in Arabidopsis Plants Overexpressing the NAD kinase2 Gene　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Kentaro Takahara; Shin-nosuke Hashida; Takayuki Hirabayashi; Tamaki Fujimori; Maki Kawai-Yamada; Tomoyuki Yamaya; Shuichi Yanagisawa; Hirofumi Uchimiya
  PLANT PHYSIOLOGY, Volume：151, Number：1, First page：100, Last page：113, Sep. 2009
  English
  DOI：https://doi.org/10.1104/pp.109.140665
  DOI ID：10.1104/pp.109.140665, ISSN：0032-0889, CiNii Articles ID：80020615084, PubMed ID：19587098, Web of Science ID：WOS:000269522200009
• A gene encoding SMALL ACIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Arabidopsis thaliana.　　　　　　　　　　　　　　　
  Akari Nakasone; Maki Kawai-Yamada; Tomohiro Kiyosue; Issay Narumi; Hirofumi Uchimiya; Yutaka Oono
  Journal of plant physiology, Volume：166, Number：12, First page：1307, Last page：13, 15 Aug. 2009, [International magazine]
  The SMALL ACIDIC PROTEIN 2 (SMAP2) gene is a paralogue of the SMAP1 gene that mediates the response to the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) in the root of Arabidopsis thaliana. Their encoded proteins, SMAP1 and SMAP2, are similar in calculated molecular weight and isoelectric point, and in having a highly conserved phenylalanine and aspartic acid-rich domain. RNA expression analysis showed that SMAP1 mRNA is present throughout the plant body while SMAP2 mRNA is restricted to siliques and anthers. Over-expression of the SMAP2 gene, as well as SMAP1, by 35S cauliflower mosaic virus promoter restored sensitivity to 2,4-D in the 2,4-D-resistant mutant, aar1, which is defective in SMAP1 function. The results suggest that SMAP2 has an ability to mediate the 2,4-D response and is expressed only in restricted tissues.
  English
  DOI：https://doi.org/10.1016/j.jplph.2009.02.005
  DOI ID：10.1016/j.jplph.2009.02.005, PubMed ID：19307045
• Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)　　　　　　　　　　　　　　　
  Minoru Nagano; Yuri Ihara-Ohori; Hiroyuki Imai; Noriko Inada; Masaru Fujimoto; Nobuhiro Tsutsumi; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANT JOURNAL, Volume：58, Number：1, First page：122, Last page：134, Apr. 2009
  English
  DOI：https://doi.org/10.1111/j.1365-313X.2008.03765.x
  DOI ID：10.1111/j.1365-313X.2008.03765.x, ISSN：0960-7412, eISSN：1365-313X, Web of Science ID：WOS:000264612800010
• 2,4‐D応答に関わるタンパク質,Small Acidic Protein 1(SMAP1)はCOP9シグナロソーム(CSN)と結合する
  中曽根光; 川合真紀; 鳴海一成; 内宮博文; 大野豊
  日本植物生理学会年会要旨集, Volume：50th, First page：250, 16 Mar. 2009
  Japanese
  J-Global ID：200902220454582383
• CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells　　　　　　　　　　　　　　　
  Juntaro Negi; Osamu Matsuda; Takashi Nagasawa; Yasuhiro Oba; Hideyuki Takahashi; Maki Kawai-Yamada; Hirofumi Uchimiya; Mimi Hashimoto; Koh Iba
  NATURE, Volume：452, Number：7186, First page：483, Last page：U13, Mar. 2008
  English
  DOI：https://doi.org/10.1038/nature06720
  DOI ID：10.1038/nature06720, ISSN：0028-0836, CiNii Articles ID：80019383908, PubMed ID：18305482, Web of Science ID：WOS:000254341300034
• The cell death factor, cell wall elicitor of rice blast fungus (Magnaporthe grisea) causes metabolic alterations including GABA shunt in rice cultured cells　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Hideo Matsumura; Maki Kawai-Yamada; Hirofumi Uchimiya
  Plant Signaling and Behavior, Volume：3, Number：11, First page：945, Last page：953, 2008
  Landes Bioscience, English
  DOI：https://doi.org/10.4161/psb.6112
  DOI ID：10.4161/psb.6112, ISSN：1559-2324, SCOPUS ID：56049093502
• The bax inhibitor-1 needs a functional electron transport chain for cell death suppression　　　　　　　　　　　　　　　
  Reiko Oshima; Keiko Yoshinaga; Yuri Ihara-Hori; Ryouichi Fukuda; Akinori Ohta; Hirofumi Uchimiya; Maki Kawai-Yamada
  FEBS LETTERS, Volume：581, Number：24, First page：4627, Last page：4632, Oct. 2007
  English
  DOI：https://doi.org/10.1016/j.febslet.2007.08.054
  DOI ID：10.1016/j.febslet.2007.08.054, ISSN：0014-5793, CiNii Articles ID：80018237639, PubMed ID：17825821, Web of Science ID：WOS:000250102900014
• Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth　　　　　　　　　　　　　　　
  Shin-nosuke Hashida; Hideyuki Takahashi; Maki Kawai-Yamada; Hirofumi Uchimiya
  PLANT JOURNAL, Volume：49, Number：4, First page：694, Last page：703, Feb. 2007
  English
  DOI：https://doi.org/10.1111/j.1365-313X.2006.02989.x
  DOI ID：10.1111/j.1365-313X.2006.02989.x, ISSN：0960-7412, Web of Science ID：WOS:000244060500010
• Mutual regulation of Arabidopsis thaliana ethylene-responsive element binding protein and a plant floral homeotic gene, APETALA2　　　　　　　　　　　　　　　
  Taro Ogawa; Hirofumi Uchimiya; Maki Kawai-Yamada
  ANNALS OF BOTANY, Volume：99, Number：2, First page：239, Last page：244, Feb. 2007
  English
  DOI：https://doi.org/10.1093/aob/mcl265
  DOI ID：10.1093/aob/mcl265, ISSN：0305-7364, PubMed ID：17204538, Web of Science ID：WOS:000244566000004
• Cell death suppressor Arabidopsis Bax inhibitor-1 is associated with calmodulin binding and ion homeostasis　　　　　　　　　　　　　　　
  Yuri Ihara-Ohori; Minoru Nagano; Shoshi Muto; Hirofumi Uchimiya; Maki Kawai-Yamada
  PLANT PHYSIOLOGY, Volume：143, Number：2, First page：650, Last page：660, Feb. 2007
  English
  DOI：https://doi.org/10.1104/pp.106.090878
  DOI ID：10.1104/pp.106.090878, ISSN：0032-0889, CiNii Articles ID：80017879340, PubMed ID：17142482, Web of Science ID：WOS:000244032400011
• Chloroplast NADK is necessary for regulation of carbon and nitrate metabolism　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Sin-nosuke Hashida; Ayumi Tanaka; Kintake Sonoike; Maki Kawai; Hirofumi Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S43, Last page：S43, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922700167
• Chloroplast NAD kinase is essential for energy transduction through the xanthophyll cycle in photosynthesis　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Ayako Watanabe; Ayumi Tanaka; Shin-nosuke Hashida; Maki Kawai-Yamada; Kintake Sonoike; Hirofumi Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：47, Number：12, First page：1678, Last page：1682, Dec. 2006
  English
  DOI：https://doi.org/10.1093/pcp/pcl029
  DOI ID：10.1093/pcp/pcl029, ISSN：0032-0781, CiNii Articles ID：10018809609, PubMed ID：17082216, Web of Science ID：WOS:000243131300010
• Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Mitsunori Hayashi; Fumiyuki Goto; Shigeru Sato; Tomoyoshi Soga; Takaaki Nishioka; Masaru Tomita; Maki Kawai-Yamada; Hirofumi Uchimiya
  ANNALS OF BOTANY, Volume：98, Number：4, First page：819, Last page：825, Oct. 2006
  English
  DOI：https://doi.org/10.1093/aob/mcl162
  DOI ID：10.1093/aob/mcl162, ISSN：0305-7364, eISSN：1095-8290, CiNii Articles ID：30022963309, PubMed ID：16849376, Web of Science ID：WOS:000240925800010
• Abolition of the tapetum suicide program ruins microsporogenesis　　　　　　　　　　　　　　　
  Takahiro Kawanabe; Tohru Ariizumi; Maki Kawai-Yamada; Hirofumi Uchimiya; Kinya Toriyama
  PLANT AND CELL PHYSIOLOGY, Volume：47, Number：6, First page：784, Last page：787, Jun. 2006
  English
  DOI：https://doi.org/10.1093/pcp/pcj039
  DOI ID：10.1093/pcp/pcj039, ISSN：0032-0781, CiNii Articles ID：10017602383, PubMed ID：16565524, Web of Science ID：WOS:000238538300013
• The mitochondrial fission regulator DRP3B does not regulate cell death in plants　　　　　　　　　　　　　　　
  Keiko Yoshinaga; Masaru Fujimoto; Shin-ichi Arimura; Nobuhiro Tsutsumi; Hirofumi Uchimiya; Maki Kawai-Yamada
  ANNALS OF BOTANY, Volume：97, Number：6, First page：1145, Last page：1149, Jun. 2006
  English
  DOI：https://doi.org/10.1093/aob/mcl057
  DOI ID：10.1093/aob/mcl057, ISSN：0305-7364, eISSN：1095-8290, CiNii Articles ID：10027345732, PubMed ID：16533833, Web of Science ID：WOS:000238816800023
• Arabidopsis transcriptional factor, AtEBP inhibited the hypersensitive response mediated by Pseudomonas syringae with an avirulent gene　　　　　　　　　　　　　　　
  T Ogawa; K Tamura; M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S47, Last page：S47, 2006
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000236401400186
• Analysis of calmodulin interacting with cell death suppressor (AtBI-1)　　　　　　　　　　　　　　　
  Z Hori; Y Ohori; H Uchimiya; M Kawai
  PLANT AND CELL PHYSIOLOGY, Volume：47, First page：S43, Last page：S43, 2006
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000236401400170
• A novel Arabidopsis gene causes Bax-like lethality in Saccharomyces cerevisiae　　　　　　　　　　　　　　　
  M Kawai-Yamada; Y Saito; LH Jin; T Ogawa; KM Kim; LH Yu; Y Tone; A Hirata; M Umeda; H Uchimiya
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：280, Number：47, First page：39468, Last page：39473, Nov. 2005
  English
  DOI：https://doi.org/10.1074/jbc.M509632200
  DOI ID：10.1074/jbc.M509632200, ISSN：0021-9258, CiNii Articles ID：80017727608, PubMed ID：16192270, Web of Science ID：WOS:000233362200068
• Mammalian Bax initiates plant cell death through organelle destruction　　　　　　　　　　　　　　　
  K Yoshinaga; S Arimura; A Hirata; Y Niwa; DJ Yun; N Tsutsumi; H Uchimiya; M Kawai-Yamada
  PLANT CELL REPORTS, Volume：24, Number：7, First page：408, Last page：417, Sep. 2005
  English
  DOI：https://doi.org/10.1007/s00299-005-0948-6
  DOI ID：10.1007/s00299-005-0948-6, ISSN：0721-7714, Web of Science ID：WOS:000232223000004
• Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana　　　　　　　　　　　　　　　
  K Yoshinaga; SI Arimura; Y Niwa; N Tsutsumi; H Uchimiya; M Kawai-Yamada
  ANNALS OF BOTANY, Volume：96, Number：2, First page：337, Last page：342, Aug. 2005
  English
  DOI：https://doi.org/10.1093/aob/mci181
  DOI ID：10.1093/aob/mci181, ISSN：0305-7364, Web of Science ID：WOS:000230724400018
• Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death　　　　　　　　　　　　　　　
  T Ogawa; L Pan; M Kawai-Yamada; LH Yu; S Yamamura; T Koyama; S Kitajima; M Ohme-Takagi; F Sato; H Uchimiya
  PLANT PHYSIOLOGY, Volume：138, Number：3, First page：1436, Last page：1445, Jul. 2005
  English
  DOI：https://doi.org/10.1104/pp.105.063586
  DOI ID：10.1104/pp.105.063586, ISSN：0032-0889, CiNii Articles ID：80017583019, PubMed ID：15980186, Web of Science ID：WOS:000230414800024
• Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice　　　　　　　　　　　　　　　
  M Hayashi; H Takahashi; K Tamura; Huang, JR; LH Yu; M Kawai-Yamada; T Tezuka; H Uchimiya
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：102, Number：19, First page：7020, Last page：7025, May 2005
  English
  DOI：https://doi.org/10.1073/pnas.0502556102
  DOI ID：10.1073/pnas.0502556102, ISSN：0027-8424, CiNii Articles ID：80017290330, PubMed ID：15863611, Web of Science ID：WOS:000229048500068
• Analysis of mitochondrial changes in Arabidopsis thaliana during ROS induced cell death　　　　　　　　　　　　　　　
  K Yoshinaga; M Kawai; S Arimura; M Fujimoto; N Tsutsumi; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S200, Last page：S200, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101303
• The stress response of Arabidopsis transcriptional factor AtEBP　　　　　　　　　　　　　　　
  T Ogawa; LH Yu; M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S199, Last page：S199, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101300
• Maize plants mutated in NAD(P)H-dependent HC-toxin reductase gene (Hm1) is vulnerable to H2O2 stress　　　　　　　　　　　　　　　
  Mitsunori Hayashi; Hideyuki Takahashi; Maki Kawai-Yamada; Takafumi Tezuka; Hirofumi Uchimiya
  Plant Biotechnology, Volume：22, Number：1, First page：69, Last page：70, 2005
  Japanese Society for Plant Cell and Molecular Biology, English
  DOI：https://doi.org/10.5511/plantbiotechnology.22.69
  DOI ID：10.5511/plantbiotechnology.22.69, ISSN：1347-6114, CiNii Articles ID：10026526470, SCOPUS ID：18744402810
• High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.)　　　　　　　　　　　　　　　
  Hideyuki Takahashi; Yuji Hotta; Mitsunori Hayashi; Maki Kawai-Yamada; Setsuko Komatsu; Hirofumi Uchimiya
  Plant Biotechnology, Volume：22, Number：1, First page：47, Last page：50, 2005
  Japanese Society for Plant Cell and Molecular Biology, English
  DOI：https://doi.org/10.5511/plantbiotechnology.22.47
  DOI ID：10.5511/plantbiotechnology.22.47, ISSN：1347-6114, CiNii Articles ID：10026526399, SCOPUS ID：18744410743
• Oxidative stress and plant cell death suppressors　　　　　　　　　　　　　　　
  Maki Kawai-Yamada; Keiko Yoshinaga; Taro Ogawa; Yuri Ihara-Ohori; Hirofumi Uchimiya
  Plant Biotechnology, Volume：22, Number：5, First page：419, Last page：422, 2005
  Japanese Society for Plant Cell and Molecular Biology, English, Book review
  DOI：https://doi.org/10.5511/plantbiotechnology.22.419
  DOI ID：10.5511/plantbiotechnology.22.419, ISSN：1347-6114, CiNii Articles ID：10028054734, SCOPUS ID：30344438808
• Isolation and characterization of Arabidopsis thaliana ISU1 gene　　　　　　　　　　　　　　　
  Y Tone; M Kawai-Yamada; H Uchimiya
  BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, Volume：1680, Number：3, First page：171, Last page：175, Nov. 2004
  English
  DOI：https://doi.org/10.1016/j.bbaexp.2004.09.005
  DOI ID：10.1016/j.bbaexp.2004.09.005, ISSN：0167-4781, Web of Science ID：WOS:000224938300004
• Bax-induced cell death of Arabidopsis is meditated through reactive oxygen-dependent and -independent processes　　　　　　　　　　　　　　　
  D Baek; J Nam; YD Koo; DH Kim; J Lee; JC Jeong; SS Kwak; WS Chung; CO Lim; JD Bahk; JC Hong; SY Lee; M Kawai-Yamada; H Uchimiya; DJ Yun
  PLANT MOLECULAR BIOLOGY, Volume：56, Number：1, First page：15, Last page：27, Sep. 2004
  English
  DOI：https://doi.org/10.1007/s11103-004-3096-4
  DOI ID：10.1007/s11103-004-3096-4, ISSN：0167-4412, CiNii Articles ID：10028054735, PubMed ID：15604726, Web of Science ID：WOS:000225899900002
• Analysis of functional domains of cell death suppressor (AtBI-1)　　　　　　　　　　　　　　　
  Y Ohori; M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S62, Last page：S62, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700244
• Functional analysis of Arabidopsis AtEBP in plants and stress responses　　　　　　　　　　　　　　　
  T Ogawa; LH Yu; M Ohme-Takagi; F Sato; M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S50, Last page：S50, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700198
• Screening of Arabidopsis genes capable of inducing yeast cell death　　　　　　　　　　　　　　　
  M Kawai; Y Saitoh; K Yoshinaga; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S62, Last page：S62, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700245
• Dissection of Arabidopsis Bax inhibitor-1 suppressing Bax-, hydrogen peroxide-, and salicylic acid-induced cell death　　　　　　　　　　　　　　　
  M Kawai-Yamada; Y Ohori; H Uchimiya
  PLANT CELL, Volume：16, Number：1, First page：21, Last page：32, Jan. 2004
  English
  DOI：https://doi.org/10.1105/tpc.014613
  DOI ID：10.1105/tpc.014613, ISSN：1040-4651, CiNii Articles ID：80016434021, PubMed ID：14671021, Web of Science ID：WOS:000188229000003
• Overexpression of Bax inhibitor suppresses the fungal elicitor-induced cell death in rice (Oryza sativa L.) cells　　　　　　　　　　　　　　　
  H Matsumura; S Nirasawa; A Kiba; N Urasaki; H Saitoh; M Ito; M Kawai-Yamada; H Uchimiya; R Terauchi
  PLANT JOURNAL, Volume：33, Number：3, First page：425, Last page：434, Feb. 2003
  English
  DOI：https://doi.org/10.1046/j.1365-313X.2003.01639.x
  DOI ID：10.1046/j.1365-313X.2003.01639.x, ISSN：0960-7412, Web of Science ID：WOS:000180851300001
• A plant gene (AtBI-1) conferring elevated tolerance to ROS-induced cell death　　　　　　　　　　　　　　　
  M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S207, Last page：S207, 2003
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000181914300821
• Cellular dissection of bax-induced cell death in plants　　　　　　　　　　　　　　　
  K Yoshinaga; M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S207, Last page：S207, 2003
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000181914300820
• Glufosinate-tolerant tobacco plants directed by the promoter of adenylate kinase gene of rice　　　　　　　　　　　　　　　
  H Fukuzawa; S Arai; M Kawai-Yamada; A Das; M Tagawa; H Uchimiya
  ANNALS OF BOTANY, Volume：89, Number：3, First page：351, Last page：354, Mar. 2002
  English
  DOI：https://doi.org/10.1093/aob2002mcf041
  DOI ID：10.1093/aob2002mcf041, ISSN：0305-7364, Web of Science ID：WOS:000174774500012
• Induction of mammalian cell death by a plant Bax inhibitor　　　　　　　　　　　　　　　
  LH Yu; M Kawai-Yamada; M Naito; K Watanabe; JC Reed; H Uchimiya
  FEBS LETTERS, Volume：512, Number：1-3, First page：308, Last page：312, Feb. 2002
  English
  DOI：https://doi.org/10.1016/S0014-5793(02)02230-5
  DOI ID：10.1016/S0014-5793(02)02230-5, ISSN：0014-5793, CiNii Articles ID：80015200913, PubMed ID：11852101, Web of Science ID：WOS:000174081900061
• Down-regulation of mammalian bax-induced plant cell death by Arabidopsis Bax inhibitor-1 (AtBI-1)　　　　　　　　　　　　　　　
  M Kawai; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S215, Last page：S215, 2002
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000174726400757
• Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses　　　　　　　　　　　　　　　
  H Uchimiya; S Fujii; Huang, JR; T Fushimi; M Nishioka; KM Kim; MK Yamada; T Kurusu; K Kuchitsu; M Tagawa
  MOLECULAR BREEDING, Volume：9, Number：1, First page：25, Last page：31, 2002
  English
  DOI：https://doi.org/10.1023/A:1019275218830
  DOI ID：10.1023/A:1019275218830, ISSN：1380-3743, Web of Science ID：WOS:000176505700003
• The Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP) can function as a dominant suppressor of Bax-induced cell death of yeast　　　　　　　　　　　　　　　
  L Pan; M Kawai; LH Yu; KM Kim; A Hirata; M Umeda; H Uchimiya
  FEBS LETTERS, Volume：508, Number：3, First page：375, Last page：378, Nov. 2001
  English
  DOI：https://doi.org/10.1016/S0014-5793(01)03098-8
  DOI ID：10.1016/S0014-5793(01)03098-8, ISSN：0014-5793, CiNii Articles ID：80012784681, PubMed ID：11728455, Web of Science ID：WOS:000172416300020
• Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBl-1)　　　　　　　　　　　　　　　
  M Kawai-Yamada; LH Jin; K Yoshinaga; A Hirata; H Uchimiya
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：98, Number：21, First page：12295, Last page：12300, Oct. 2001
  English
  DOI：https://doi.org/10.1073/pnas.211423998
  DOI ID：10.1073/pnas.211423998, ISSN：0027-8424, CiNii Articles ID：80012776846, PubMed ID：11593047, Web of Science ID：WOS:000171558900085
• 植物の形態形成分子機構の研究 イオンビームによる植物細胞アポトーシス誘導体の確立
  川合真紀; 小林泰彦; 大野豊; 渡辺宏; 内宮博文
  基礎科学ノート, Volume：8, Number：1, First page：9, Last page：12, Mar. 2001
  Japanese
  ISSN：1340-3079, J-Global ID：200902118512698355
• Nucleoside diphosphate kinase required for coleoptile elongation in rice　　　　　　　　　　　　　　　
  L Pan; M Kawai; A Yano; H Uchimiya
  PLANT PHYSIOLOGY, Volume：122, Number：2, First page：447, Last page：452, Feb. 2000
  English
  DOI：https://doi.org/10.1104/pp.122.2.447
  DOI ID：10.1104/pp.122.2.447, ISSN：0032-0889, eISSN：1532-2548, CiNii Articles ID：80011516620, PubMed ID：10677437, Web of Science ID：WOS:000086903000014
• Coleoptile senescence in rice (Oryza sativa L.)　　　　　　　　　　　　　　　
  Maki Kawai; Hirofumi Uchimiya
  Annals of Botany, Volume：86, Number：2, First page：405, Last page：414, 2000
  Academic Press, English
  DOI：https://doi.org/10.1006/anbo.2000.1199
  DOI ID：10.1006/anbo.2000.1199, ISSN：0305-7364, SCOPUS ID：0033832231
• Ion beam as a noble tool to induce apoptosis-like cell death in roots of maize (Zea mays L.)　　　　　　　　　　　　　　　
  Maki Kawai; Yasuhiko Kobayashi; Aiko Hirata; Yutaka Oono; Hiroshi Watanabe; Hirofumi Uchimiya
  Plant Biotechnology, Volume：17, Number：4, First page：305, Last page：308, 2000
  DOI：https://doi.org/10.5511/plantbiotechnology.17.305
  Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0000853046&origin=inward
  Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0000853046&origin=inward
  DOI ID：10.5511/plantbiotechnology.17.305, ISSN：1342-4580, eISSN：1347-6114, CiNii Articles ID：10010147080, SCOPUS ID：0000853046
• Transverse vein differentiation associated with gas space formation - Fate of the middle cell layer in leaf sheath development of rice　　　　　　　　　　　　　　　
  C Matsukura; M Kawai; K Toyofuku; RA Barrero; H Uchimiya; J Yamaguchi
  ANNALS OF BOTANY, Volume：85, Number：1, First page：19, Last page：27, Jan. 2000
  English
  DOI：https://doi.org/10.1006/anbo.1999.0993
  DOI ID：10.1006/anbo.1999.0993, ISSN：0305-7364, Web of Science ID：WOS:000084991000004
• Evolutionally conserved plant homologue of the Bax Inhibitor-1 (BI-1) gene capable of suppressing Bax-induced cell death in yeast　　　　　　　　　　　　　　　
  M Kawai; L Pan; JC Reed; H Uchimiya
  FEBS LETTERS, Volume：464, Number：3, First page：143, Last page：147, Dec. 1999
  English
  DOI：https://doi.org/10.1016/S0014-5793(99)01695-6
  DOI ID：10.1016/S0014-5793(99)01695-6, ISSN：0014-5793, CiNii Articles ID：80011426952, PubMed ID：10618494, Web of Science ID：WOS:000084654800009
• TRANSVERSE VEIN DIFFERENTIATION ASSOCIATED WITH THE AIR SPACE FORMATION - CELL FATE OF MIDDLE LAYER IN LEAF SHEATH DEVELOPMENT OF RICE -　　　　　　　　　　　　　　　
  MATSUKURA Chiaki; KAWAI Maki; TOYOFUKU Kyoko; BARRERO Robert A.; UCHIMIYA Hirofumi; YAMAGUCHI Junji
  Volume：40, First page：s15, Last page：s15, Mar. 1999
  English
  ISSN：0032-0781, CiNii Articles ID：10003757764, CiNii Books ID：AA0077511X
• Morphological analysis of apoptosis-like cell death induced by ion beam irradiation in plant cells　　　　　　　　　　　　　　　
  First page：54, Last page：55, 1999
• Cortical cell death, cell proliferation, macromolecular movements and rTip1 expression pattern in roots of rice (Oryza sativa L,) under NaCl stress　　　　　　　　　　　　　　　
  PK Samarajeewa; RA Barrero; C Umeda-Hara; M Kawai; H Uchimiya
  PLANTA, Volume：207, Number：3, First page：354, Last page：361, Jan. 1999
  English
  DOI：https://doi.org/10.1007/s004250050492
  DOI ID：10.1007/s004250050492, ISSN：0032-0935, Web of Science ID：WOS:000078274900004
• Morphological analysis of apoptosis-like cell death induced by ion beam irradiation in plant cells　　　　　　　　　　　　　　　
  JAERI report 1999, First page：54, Last page：55, 1999
• Isolation of a cotton CAP gene: a homologue of adenylyl cyclase-associated protein highly expressed during fiber elongation　　　　　　　　　　　　　　　
  M Kawai; S Aotsuka; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：39, Number：12, First page：1380, Last page：1383, Dec. 1998
  English
  ISSN：0032-0781, Web of Science ID：WOS:000077761600019
• Stimulation of adenylate kinase in rice seedlings under submergence stress　　　　　　　　　　　　　　　
  M Kawai; M Umeda; H Uchimiya
  JOURNAL OF PLANT PHYSIOLOGY, Volume：152, Number：4-5, First page：533, Last page：539, May 1998
  English
  ISSN：0176-1617, Web of Science ID：WOS:000073921400029
• Cellular dissection of the degradation pattern of cortical cell death during aerenchyma formation of rice roots　　　　　　　　　　　　　　　
  M Kawai; PK Samarajeewa; RA Barrero; M Nishiguchi; H Uchimiya
  PLANTA, Volume：204, Number：3, First page：277, Last page：287, Mar. 1998
  English
  DOI：https://doi.org/10.1007/s004250050257
  DOI ID：10.1007/s004250050257, ISSN：0032-0935, CiNii Articles ID：10009945397, Web of Science ID：WOS:000072453800002
• Induction of apoptosis in plant cells by ion beam irradiation　　　　　　　　　　　　　　　
  First page：57, 1998
• Induction of apoptosis in plant cells by ion beam irradiation　　　　　　　　　　　　　　　
  JAERI report 1998, First page：57, 1998
• Genetic engineering for abiotic stress tolerance in plants　　　　　　　　　　　　　　　
  Harcharan S. Dhariwal; Maki Kawai; Hirofumi Uchimiya
  Plant Biotechnology, Volume：15, Number：1, First page：1, Last page：10, 1998
  Japanese Society for Plant Cell and Molecular Biology, English, Book review
  DOI：https://doi.org/10.5511/plantbiotechnology.15.1
  DOI ID：10.5511/plantbiotechnology.15.1, ISSN：1347-6114, CiNii Articles ID：10006290826, SCOPUS ID：54649083917
• ANALYSIS OF AERENCHYMA FORMATION CAUSED BY CELL DEATH IN RICE ROOT　　　　　　　　　　　　　　　
  KAWAI Maki; SAMARAJEEWA P. K.; YAMAGUCHI Masatoshi; UCHIMIYA Hirofumi
  Volume：37, First page：131, Last page：131, Mar. 1996
  English
  ISSN：0032-0781, CiNii Articles ID：10002708306, CiNii Books ID：AA0077511X
• TISSUE-SPECIFIC LOCALIZATION OF ADENYLATE KINASE IN RICE (ORYZA-SATIVA L) PLANTS　　　　　　　　　　　　　　　
  M KAWAI; H UCHIMIYA
  JOURNAL OF PLANT PHYSIOLOGY, Volume：146, Number：3, First page：239, Last page：242, Jun. 1995
  English
  ISSN：0176-1617, Web of Science ID：WOS:A1995RJ37600008
• BIOCHEMICAL-PROPERTIES OF RICE ADENYLATE KINASE AND SUBCELLULAR LOCATION IN PLANT-CELLS　　　　　　　　　　　　　　　
  M KAWAI; H UCHIMIYA
  PLANT MOLECULAR BIOLOGY, Volume：27, Number：5, First page：943, Last page：951, Mar. 1995
  English
  ISSN：0167-4412, Web of Science ID：WOS:A1995RB39500009
• Sodium Chloride Stimulates Adenylate Kinase Level in Seedlings of Salt-Sensitive Rice Varieties　　　　　　　　　　　　　　　
  Pallepitiya K. Samarajeewa; Maki Kawai; Toyoaki Anai; Atsushi Hirai; Hirofumi Uchimiya
  Journal of Plant Physiology, Volume：147, Number：2, First page：277, Last page：280, 1995
  English
  DOI：https://doi.org/10.1016/S0176-1617(11)81519-1
  DOI ID：10.1016/S0176-1617(11)81519-1, ISSN：0176-1617, SCOPUS ID：0029190285
• Tissus specific localization of adenylate kinase in rice(Oryza sativa L.) plants　　　　　　　　　　　　　　　
  J.Plant Phys., Volume：146, First page：239, Last page：243, 1995
• Molecular characterization of cDNAencoing for adenylate kinase of rice(Oryza sative L.)　　　　　　　　　　　　　　　
  Volume：2, First page：845, Last page：854, 1992
• Molecular characterization of cDNAencoing for adenylate kinase of rice(Oryza sative L.)　　　　　　　　　　　　　　　
  Plant J., Volume：2, First page：845, Last page：854, 1992

• THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
• JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY
• THE BOTANICAL SOCIETY OF JAPAN
• THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

• 光合成の多様性を支える電子受容体NADP供給系の変容　　　　　　　　　　　　　　　
  01 Apr. 2024 - 31 Mar. 2026
  Grant amount(Total)：7410000, Direct funding：5700000, Indirect funding：1710000
  Grant number：24H02065
• 植物環境応答におけるNAD(P)(H)代謝ネットワーク制御の分子基盤　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2026
  Grant amount(Total)：17160000, Direct funding：13200000, Indirect funding：3960000
  Grant number：23K23564
• 植物環境応答におけるNAD(P)(H)代謝ネットワーク制御の分子基盤　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2026
  Grant amount(Total)：17160000, Direct funding：13200000, Indirect funding：3960000
  Grant number：22H02298
• 酸化還元バランス制御を介した不均一環境への応答機構　　　　　　　　　　　　　　　
  01 Apr. 2023 - 31 Mar. 2025
  Grant amount(Total)：7800000, Direct funding：6000000, Indirect funding：1800000
  Grant number：23H04187
• 植物の不均一環境変動応答を支える細胞内酸化還元力バランス制御　　　　　　　　　　　　　　　
  10 Sep. 2021 - 31 Mar. 2023
  Grant amount(Total)：9360000, Direct funding：7200000, Indirect funding：2160000
  Grant number：21H05647
• 葉緑体機能を支えるレドックス制御システムの包括的解析　　　　　　　　　　　　　　　
  01 Apr. 2019 - 31 Mar. 2023
  Grant amount(Total)：17160000, Direct funding：13200000, Indirect funding：3960000
  Grant number：19H03241
• Research on microdomain visualization probes to elucidate plant immunity　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), 29 Jun. 2018 - 31 Mar. 2022
  Kawai-Yamada Maki, Saitama University
  Grant amount(Total)：6370000, Direct funding：4900000, Indirect funding：1470000
  In order to examine the functions of microdomains scattered on the plasma membrane, which play an important role in plant disease response, we have developed plant microdomain visualization probes targeting their major constituent lipids. We developed a visualization probe, GFP-D4L, which binds to phytosterols, and succeeded in visualizing microdomain structures on the plasma membrane. We also generated an Arabidopsis line that constitutively expresses GFP-D4L and showed that the microdomain exhibits specific dynamics during disease response.
  Grant number：18K19164
• 植物の環境ストレス応答における生体膜マイクロドメインの構築と機能　　　　　　　　　　　　　　　
  01 Apr. 2018 - 31 Mar. 2022
  Grant amount(Total)：17290000, Direct funding：13300000, Indirect funding：3990000
  Grant number：18H02165
• 葉緑体NADP供給とプロトン駆動力のバランス制御機構の解明　　　　　　　　　　　　　　　
  01 Apr. 2019 - 31 Mar. 2021
  Grant amount(Total)：7540000, Direct funding：5800000, Indirect funding：1740000
  Grant number：19H04715
• プロトン駆動力とNADP量的制御のクロストーク　　　　　　　　　　　　　　　
  01 Apr. 2017 - 31 Mar. 2019
  Grant amount(Total)：8320000, Direct funding：6400000, Indirect funding：1920000
  Grant number：17H05714
• Analysis of plant environmental response through plasmamembrane microdomain　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2014 - 31 Mar. 2019
  KAWAI-YAMADA Maki, Saitama University
  Grant amount(Total)：17160000, Direct funding：13200000, Indirect funding：3960000
  Plants respond to various environmental stresses through plasmamembrane microdomains. In our previous studied, we demonstrated that the overexpression of Arabidopsis BI-1, which localized on ER membrane, appeared enhanced tolerance to oxidative stress. In this research project, we characterized FAH, ELO, and SLD proteins, which are isolated as interacting factors to BI-1, directly or indirectly. Interestingly, these factors are related to the sphingolipid metabolism. The sphingolipid is known to be involved in the plasmamembrane microdomain. In this study, we produced transgenic plants in which these genes were over-expressing or knock-down. These plants showed modified sphingolipid profiles and different responses to oxidative stresses, suggest that the involvement of sphingolipid metabolism to the plat environmental responses.
  Grant number：26292190
• Analysis of GHG emission dynamics in hydroponics　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 2010 - 2012
  YOSHIHARA Toshihiro; HASHIDA Shin-nosuke; GOTO Fumiyuki; SHOJI Kazuhiro; UEMOTO Hiroaki; JOKAN Masafumi; KAWAI Maki, Central Research Institute of Electric Power Industry
  Grant amount(Total)：19370000, Direct funding：14900000, Indirect funding：4470000
  A rockwool based nutrient dripping culture system for tomato was used as a model to clarifychanges in GHG emissions (CO_2, N_2O, and CH_4) from the rhizosphere. The points of the results are as follows: 1. The GHG emissions were highly dependent on the environmental conditions,such as temperature, irradiation, and fertilization. 2. The changes in GHG emission duringthe culture were almost the same between the rockwool culture and a traditional soilculture, although the total GHG emission was basically larger in the soil culture thanin the rockwool culture. 3. Changes in micro floras and the amounts were not correlatedwith the changes in N_2O emission. In particular, we established a continuous and real-timemeasurement system for N_2O emission, and newly discovered a very quick response of theN_2O emission to the fertilization (i.e., The N2O emission began to increase at least within10 to 15 min and peaked around 30 to 60 min from the beginning of each nutrient supply,and backed to the background level at most within 7 h after the end of the supply).
  Grant number：22380139
• 脂肪酸代謝をエフェクターとする植物酸化ストレス応答細胞死の機構　　　　　　　　　　　　　　　
  2009 - 2010
  Grant amount(Total)：6110000, Direct funding：4700000, Indirect funding：1410000
  Grant number：21117505
• -　　　　　　　　　　　　　　　
  Competitive research funding
• -　　　　　　　　　　　　　　　
  Competitive research funding