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小川 哲史(オガワ サトシ)
理工学研究科 生命科学部門 | 准教授(特定プロジェクト教員) |
理学部 分子生物学科 |
- プロフィール:
2011年東京大学農学部卒業。2017年東京大学大学院にて博士(農学)取得。2017年より理化学研究所にて特別研究員、2021年より同訪問研究員、2023年より2024年まで同学振特別研究員PD。2021年より2024年まで日本学術振興会特別研究員、2022年より2024年までカリフォルニア大学リバーサイド校客員研究員を兼任。2024年より京都大学にて特定助教。2025年より現職。一貫して植物が外部のシグナルに応答する分子機構の研究に従事。2023年、理研研究奨励賞(桜舞賞)受賞。
研究者情報
■ 学位■ 研究キーワード
■ 研究分野
■ 経歴
- 2025年04月 - 現在, 埼玉大学, 大学院理工学研究科, 准教授, 日本国
- 2024年04月 - 2025年03月, 京都大学, 化学研究所, 特定助教
- 2023年10月 - 2024年03月, 特定国立研究開発法人理化学研究所, 環境資源科学研究センター, 学振特別研究員PD
- 2022年08月 - 2024年03月, カリフォルニア大学リバーサイド校, 客員研究員, アメリカ合衆国
- 2021年04月 - 2024年03月, 独立行政法人日本学術振興会, 特別研究員, 日本国
- 2021年04月 - 2023年09月, 特定国立研究開発法人理化学研究所, 環境資源科学研究センター, 訪問研究員, 日本国
- 2017年04月 - 2021年03月, 特定国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員, 日本国
- 2011年04月 - 2017年03月, 東京大学, 大学院農学生命科学研究科, 応用生命工学専攻, 日本国
- 2009年04月 - 2011年03月, 東京大学, 農学部, 応用生命科学課程
- 2007年04月 - 2009年03月, 東京大学, 教養学部, 理科二類
- 2023年03月, 第14回 理研研究奨励賞(桜舞賞), 寄生植物が宿主に接近するための分子機構の解明, 国立研究開発法人理化学研究所
小川 哲史
35768353 - 2018年11月, Best Poster Award in FY2018 CSRS Interim Progress Report, Expression dynamics of subtilases in the haustorium of the parasitic plant Phtheirospermum japonicum, 理化学研究所 環境資源科学研究センター
Satoshi Ogawa;Takanori Wakatake;Juliane K. Ishida;Satoko Yoshida;Yasunori Ichihashi;Ken Shirasu
業績情報
■ 論文- Ethylene promotes SMAX1 accumulation to inhibit arbuscular mycorrhiza symbiosis.
Debatosh Das; Kartikye Varshney; Satoshi Ogawa; Salar Torabi; Regine Hüttl; David C Nelson; Caroline Gutjahr
Nature Communications, 巻:16, 号:1, 開始ページ:2025, 終了ページ:2025, 2025年02月, [査読有り], [国際誌]
Most land plants engage in arbuscular mycorrhiza (AM) symbiosis with Glomeromycotina fungi for better access to mineral nutrients. The plant hormone ethylene suppresses AM development, but a molecular explanation for this phenomenon is lacking. Here we show that ethylene inhibits the expression of many genes required for AM formation in Lotus japonicus. These genes include strigolactone biosynthesis genes, which are needed for fungal activation, and Common Symbiosis genes, which are required for fungal entry into the root. Application of strigolactone analogs and ectopic expression of the Common Symbiosis gene Calcium Calmodulin-dependent Kinase (CCaMK) counteracts the effect of ethylene. Therefore, ethylene likely inhibits AM development by suppressing expression of these genes rather than by inducing defense responses. These same genes are regulated by SUPPRESSOR OF MAX2 1 (SMAX1), a transcriptional repressor that is proteolyzed during karrikin signaling. SMAX1 is required for suppression of AM by ethylene, and SMAX1 abundance in nuclei increases after ethylene application. We conclude that ethylene suppresses AM by promoting accumulation of SMAX1. SMAX1 emerges as a signaling hub that integrates karrikin and ethylene signaling, thereby orchestrating development of a major plant symbiosis with a plant's physiological state.
英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1038/s41467-025-57222-w
DOI ID:10.1038/s41467-025-57222-w, PubMed ID:40016206, 共同研究・競争的資金等ID:35768353 - Dominant-negative KAI2d paralogs putatively attenuate strigolactone responses in root parasitic plants
Alexandra R.F White; Annalise Kane; Satoshi Ogawa; Ken Shirasu; David C Nelson
Plant And Cell Physiology, 2024年09月, [査読有り]
ABSTRACT
Many root parasitic plants in the Orobanchaceae use host-derived strigolactones as germination cues. This adaptation facilitates attachment to a host and is particularly important for the success of obligate parasitic weeds that cause substantial crop losses globally. Parasite seeds sense strigolactones through “divergent” KARRIKIN INSENSITIVE2 (KAI2d)/HYPOSENSITIVE TO LIGHT (HTL) α/β-hydrolases that have undergone substantial duplication and diversification in Orobanchaceae genomes. After germination, chemotropic growth of parasite roots toward a strigolactone source also occurs in some species. We investigated which of the seven KAI2d genes found in a facultative hemiparasite, Phtheirospermum japonicum, may enable chemotropic responses to strigolactones. To do so, we developed a triple mutant Nbd14a,b kai2i line of Nicotiana benthamiana in which strigolactone-induced degradation of SMAX1, an immediate downstream target of KAI2 signaling, is disrupted. In combination with a transiently expressed, ratiometric reporter of SMAX1 protein abundance, this mutant forms a system for the functional analysis of parasite KAI2d proteins in a plant cellular context. Using this system, we unexpectedly found three PjKAI2d proteins that do not trigger SMAX1 degradation in the presence of strigolactones. Instead, these PjKAI2d inhibit the perception of low strigolactone concentrations by strigolactone-responsive PjKAI2d in a dominant-negative manner that depends upon an active catalytic triad. Similar dominant-negative KAI2d paralogs were identified in an obligate hemiparasitic weed, Striga hermonthica. These proteins suggest a mechanism for attenuating strigolactone signaling in parasites, which might be used to enhance the perception of shallow strigolactone gradients during root growth toward a host or to restrict germination responses to specific strigolactones.
Oxford University Press (OUP), 研究論文(学術雑誌)
DOI:https://doi.org/10.1093/pcp/pcae106
DOI ID:10.1093/pcp/pcae106, ISSN:0032-0781, eISSN:1471-9053, 共同研究・競争的資金等ID:35768353 - Strigolactones in Rhizosphere Communication: Multiple Molecules With Diverse Functions
Yee Jia Kee; Satoshi Ogawa; Yasunori Ichihashi; Ken Shirasu; Satoko Yoshida
Plant And Cell Physiology, 2023年06月, [査読有り], [筆頭著者]
Abstract
Strigolactones (SLs) are root-secreted small molecules that influence organisms living in the rhizosphere. While SLs are known as germination stimulants for root parasitic plants and as hyphal branching factors for arbuscular mycorrhizal fungi, recent studies have also identified them as chemoattractants for parasitic plants, sensors of neighboring plants and key players in shaping the microbiome community. Furthermore, the discovery of structurally diverged SLs, including so-called canonical and non-canonical SLs in various plant species, raises the question of whether the same SLs are responsible for their diverse functions ‘in planta’ and the rhizosphere or whether different molecules play different roles. Emerging evidence supports the latter, with each SL exhibiting different activities as rhizosphere signals and plant hormones. The evolution of D14/KAI2 receptors has enabled the perception of various SLs or SL-like compounds to control downstream signaling, highlighting the complex interplay between plants and their rhizosphere environment. This review summarizes the recent advances in our understanding of the diverse functions of SLs in the rhizosphere.
Oxford University Press (OUP), 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1093/pcp/pcad055
DOI ID:10.1093/pcp/pcad055, ISSN:0032-0781, eISSN:1471-9053, 共同研究・競争的資金等ID:35768353 - Strigol induces germination of the facultative parasitic plant Phtheirospermum japonicum in the absence of nitrate ions.
Satoshi Ogawa; Ken Shirasu
Plant Signaling & Behavior, 巻:17, 号:1, 開始ページ:2114647, 2022年12月, [査読有り], [筆頭著者, 責任著者], [国際誌]
Root parasitic plants in the family Orobanchaceae, such as Striga and Orobanche spp., infest major crops worldwide, leading to a multibillion-dollar loss annually. Host-derived strigolactones (SLs), recognized by a group of α/β hydrolase receptors (KAI2d) in these parasites, are important determinants for germinating root parasitic plants near the roots of host plants. Phtheirospermum japonicum, a facultative hemiparasitic Orobanchaceae plant, can germinate and grow in the presence or absence of the host and can also exhibit root chemotropism to host-derived SLs that are perceived via KAI2d. However, the importance of SLs in P. japonicum germination remains unclear. In this study, we found that germination of P. japonicum was suppressed in the absence of nitrate ions and that germination of P. japonicum was promoted by exogenous strigol, an SL, under such conditions. We propose a model in which P. japonicum may select either independent living or parasitism in response to ambient nitrogen conditions and host presence.
英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1080/15592324.2022.2114647
DOI ID:10.1080/15592324.2022.2114647, PubMed ID:35993137, 共同研究・競争的資金等ID:35768353 - Strigolactones are chemoattractants for host tropism in Orobanchaceae parasitic plants.
Satoshi Ogawa; Songkui Cui; Alexandra R F White; David C Nelson; Satoko Yoshida; Ken Shirasu
Nature Communications, 巻:13, 号:1, 開始ページ:4653, 終了ページ:4653, 2022年08月, [査読有り], [筆頭著者], [国際誌]
Parasitic plants are worldwide threats that damage major agricultural crops. To initiate infection, parasitic plants have developed the ability to locate hosts and grow towards them. This ability, called host tropism, is critical for parasite survival, but its underlying mechanism remains mostly unresolved. To characterise host tropism, we used the model facultative root parasite Phtheirospermum japonicum, a member of the Orobanchaceae. Here, we show that strigolactones (SLs) function as host-derived chemoattractants. Chemotropism to SLs is also found in Striga hermonthica, a parasitic member of the Orobanchaceae, but not in non-parasites. Intriguingly, chemotropism to SLs in P. japonicum is attenuated in ammonium ion-rich conditions, where SLs are perceived, but the resulting asymmetrical accumulation of the auxin transporter PIN2 is diminished. P. japonicum encodes putative receptors that sense exogenous SLs, whereas expression of a dominant-negative form reduces its chemotropic ability. We propose a function for SLs as navigators for parasite roots.
英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1038/s41467-022-32314-z
DOI ID:10.1038/s41467-022-32314-z, PubMed ID:35970835, PubMed Central ID:PMC9378612, 共同研究・競争的資金等ID:35768353 - Subtilase activity in intrusive cells mediates haustorium maturation in parasitic plants
Satoshi Ogawa; Takanori Wakatake; Thomas Spallek; Juliane K. Ishida; Ryosuke Sano; Tetsuya Kurata; Taku Demura; Satoko Yoshida; Yasunori Ichihashi; Andreas Schaller; Ken Shirasu
Plant Physiology, 巻:185, 号:4, 開始ページ:1381, 終了ページ:1394, 2020年12月, [査読有り], [筆頭著者], [国際誌]ABSTRACT Parasitic plants that infect crops are devastating to agriculture throughout the world. They develop a unique inducible organ called the haustorium, which connects the vascular systems of the parasite and host to establish a flow of water and nutrients. Upon contact with the host, the haustorial epidermal cells at the interface with the host differentiate into specific cells called intrusive cells that grow endophytically towards the host vasculature. Then, some of the intrusive cells re-differentiate to form a xylem bridge that connects the vasculatures of the parasite and host. Despite the prominent role of intrusive cells in host infection, the molecular mechanisms mediating parasitism in the intrusive cells are unknown. In this study, we investigated differential gene expression in the intrusive cells of the facultative parasitePhtheirospermum japonicum in the family Orobanchaceae by RNA-Sequencing of laser-microdissected haustoria. We then used promoter analyses to identify genes that are specifically induced in intrusive cells, and used promoter fusions with genes encoding fluorescent proteins to develop intrusive cell-specific markers. Four of the intrusive cell-specific genes encode subtilisin-like serine proteases (SBTs), whose biological functions in parasitic plants are unknown. Expression of an SBT inhibitor in the intrusive cells inhibited their development, inhibited the development of the xylem bridge, and reduced auxin response levels near the site where the xylem bridge normally develops. Therefore, we propose that subtilase activity plays an important role in haustorium development in this parasitic plant.One sentence summary Tissue-specific analysis showed that the subtilases specifically expressed in intrusive cells regulate auxin-mediated host-parasite connections in the parasitic plantPhtheirospermum japonicum .
Cold Spring Harbor Laboratory, 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1101/2020.03.30.015149
DOI ID:10.1101/2020.03.30.015149, PubMed ID:33793894, PubMed Central ID:PMC8133603 - Host-parasite tissue adhesion by a secreted type of β-1,4-glucanase in the parasitic plant Phtheirospermum japonicum
Ken-ichi Kurotani; Takanori Wakatake; Yasunori Ichihashi; Koji Okayasu; Yu Sawai; Satoshi Ogawa; Songkui Cui; Takamasa Suzuki; Ken Shirasu; Michitaka Notaguchi
Communications Biology, 巻:3, 号:1, 2020年12月, [査読有り]
Tissue adhesion between plant species occurs both naturally and artificially. Parasitic plants establish intimate relationship with host plants by adhering tissues at roots or stems. Plant grafting, on the other hand, is a widely used technique in agriculture to adhere tissues of two stems. Here we found that the model Orobanchaceae parasitic plant Phtheirospermum japonicum can be grafted on to interfamily species. To understand molecular basis of tissue adhesion between distant plant species, we conducted comparative transcriptome analyses on both infection and grafting by P. japonicum on Arabidopsis. Despite different organs, we identified the shared gene expression profile, where cell proliferation- and cell wall modification-related genes are up-regulated. Among genes commonly induced in tissue adhesion between distant species, we showed a gene encoding a secreted type of beta -1,4-glucanase plays an important role for plant parasitism. Our data provide insights into the molecular commonality between parasitism and grafting in plants. Ken-ichi Kurotani, Takanori Wakatake et al. study tissue adhesion in plants. They found that the model Orobanchaceae parasitic plant Phtheirospermum japonicum can be grafted on to interfamily species. Using transcriptome profiling, the identify genes involved in tissue adhesion between distant species and demonstrate that beta -1,4-glucanase plays an important role in plant parasitism.
Springer Science and Business Media LLC, 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1038/s42003-020-01143-5
DOI ID:10.1038/s42003-020-01143-5, eISSN:2399-3642, Web of Science ID:WOS:000559022600005 - Auxin transport network underlies xylem bridge formation between the hemi-parasitic plant Phtheirospermum japonicum and host Arabidopsis.
Takanori Wakatake; Satoshi Ogawa; Satoko Yoshida; Ken Shirasu
Development (Cambridge, England), 巻:147, 号:14, 2020年06月, [査読有り], [国際誌]
Parasitic plants form vascular connections to host plants for efficient material transport. The haustorium is the responsible organ for host invasion and subsequent vascular connection. After invasion of host tissues, vascular meristem-like cells emerge in the central region of the haustorium, differentiate into tracheary elements, and establish a connection, known as a xylem bridge, between parasite and host xylem systems. Despite the importance of this parasitic connection, the regulatory mechanisms of xylem bridge formation are unknown. Here we show the role of auxin and auxin transporters during the process of xylem bridge formation using an Orobanchaceae hemiparasitic plant, Phtheirospermum japonicum The auxin response marker DR5 has a similar expression pattern to tracheary element differentiation genes in haustoria. Auxin transport inhibitors alter tracheary element differentiation in haustoria, but biosynthesis inhibitors do not, demonstrating the importance of auxin transport during xylem bridge formation. The expression patterns and subcellular localization of PIN family auxin efflux carriers and AUX1/LAX influx carriers correlate with DR5 expression patterns. The cooperative action of auxin transporters is therefore responsible for controlling xylem vessel connections between parasite and host.
英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1242/dev.187781
DOI ID:10.1242/dev.187781, PubMed ID:32586973 - OsMYC2 mediates numerous defence-related transcriptional changes via jasmonic acid signalling in rice
Satoshi Ogawa; Ryouka Kawahara-Miki; Koji Miyamoto; Hisakazu Yamane; Hideaki Nojiri; Yoshimasa Tsujii; Kazunori Okada
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 巻:486, 号:3, 開始ページ:796, 終了ページ:803, 2017年05月, [査読有り], [筆頭著者]
Jasmonic acid (JA) plays central roles in various events in plants, especially defence against pathogens and insects. The basic helix-loop-helix (bHLH) transcription factor MYC2 has attracted attention as a master regulator of JA signalling in dicotyledonous plants. However, how MYC2 functions in monocotyledonous plants, including agriculturally important crops such as cultivated rice, has been poorly understood. To elucidate the comprehensive effects of rice MYC2 (OsMYC2) on the JA-inducible transcriptional modifications, we performed RNA-sequencing by using OsMYC2-knockdown plants (osmyc2RNAi). In osmyc2RNAi, JA-inducible expression of many defence-related genes, for example chitinases and proteinase inhibitors, was compromised. Decrease in JA-dependent activation of the biosynthetic pathways of specialised metabolites, especially defence compounds, was also evident in the osmyc2RNAi line. Furthermore, a substantial change was noted in the expression of distinct types of transcription factors, such as MYB-type factors, likely depicting the importance of OsMYC2 in not only defence responses but also other morphogenetic events. Our findings provide fundamental information to understand the overall functions of MYC2 in JA signalling in monocotyledonous plants, which might yield agricultural benefits. (C) 2017 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1016/j.bbrc.2017.03.125
DOI ID:10.1016/j.bbrc.2017.03.125, ISSN:0006-291X, eISSN:1090-2104, Web of Science ID:WOS:000399966700030 - OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability
Satoshi Ogawa; Koji Miyamoto; Keiichirou Nemoto; Tatsuya Sawasaki; Hisakazu Yamane; Hideaki Nojiri; Kazunori Okada
SCIENTIFIC REPORTS, 巻:7, 2017年01月, [査読有り], [筆頭著者]
Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice.
NATURE PUBLISHING GROUP, 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1038/srep40175
DOI ID:10.1038/srep40175, ISSN:2045-2322, Web of Science ID:WOS:000391759200001 - Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice
Tetsuya Chujo; Koji Miyamoto; Satoshi Ogawa; Yuka Masuda; Takafumi Shimizu; Mitsuko Kishi-Kaboshi; Akira Takahashi; Yoko Nishizawa; Eiichi Minami; Hideaki Nojiri; Hisakazu Yamane; Kazunori Okada
PLOS ONE, 巻:9, 号:6, 2014年06月, [査読有り]
WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.
PUBLIC LIBRARY SCIENCE, 英語, 研究論文(学術雑誌)
DOI:https://doi.org/10.1371/journal.pone.0098737
DOI ID:10.1371/journal.pone.0098737, ISSN:1932-6203, Web of Science ID:WOS:000336911400087
- Identification of key genetic player candidates in the biosynthesis of a putative plant hormone in Arabidopsis thaliana
Satoshi Ogawa; Kiyoshi Mashiguchi; Shinjiro Yamaguchi; David C. Nelson
第66回日本植物生理学会年会, 開始ページ:2P107, 2025年03月15日, [筆頭著者]
英語, 会議報告等
共同研究・競争的資金等ID:47156465 - KAI2を介して信号伝達を行う未知植物ホルモンの生合成遺伝子候補の解析
徐千帆; 小川哲史; 増口潔; 山口信次郎; David C. Nelson
第66回日本植物生理学会年会, 開始ページ:2P108, 2025年03月15日
英語, 会議報告等
共同研究・競争的資金等ID:47156465 - SMAX1 is a Signaling Hub That Integrates Karrikin- and Ethylene-mediated Regulation of Seedling Photomorphogenesis in Arabidopsis thaliana
Satoshi Ogawa; Caroline Gutjahr; David C. Nelson
日本植物生理学会年会(Web) 65th, 開始ページ:2P50, 2024年03月18日, [筆頭著者]
英語, 会議報告等
共同研究・競争的資金等ID:35768353 - Interaction of phytohormone signaling pathways regulating root colonization by arbuscular mycorrhizal fungi
Debatosh Das; Kartikye Varshney; Satoshi Ogawa; Salar Torabi; Regina Hüttl; David Nelson; Caroline Gutjahr
2023年07月, [招待有り]
英語
共同研究・競争的資金等ID:35768353 - ハマウツボ科寄生植物の宿主屈性に関する分子機構の解明
小川哲史; Songkui Cui; 吉田 聡子; 白須賢
日本植物生理学会年会(Web), 巻:63rd, 2022年03月, [筆頭著者]
共同研究・競争的資金等ID:35768353 - 寄生植物コシオガマの吸器形成におけるsubtilaseの役割
Satoshi Ogawa; Takanori Wakatake; Thomas SPALLEK; Juliane K. Ishida; Ryosuke Sano; Tetsuya Kurata; Taku Demura; Satoko Yoshida; Yasunori Ichihashi; Andreas Schaller; Ken Shirasu
日本植物生理学会年会(Web), 巻:62nd, 2021年, [筆頭著者]
J-Global ID:202102283398226677 - Parasitism-inducible proteases and their putative peptide hormone in the haustorium of the parasitic plant Phtheirospermum japonicum
Satoshi Ogawa; Takanori Wakatake; Juliane K. Ishida; Satoko Yoshida; Thomas Spallek; Yasunori Ichihashi; Ken Shirasu
2019年06月, [査読有り], [筆頭著者] - 寄生植物コシオガマの吸器におけるsubtilaseの発現動態解析
OGAWA Satoshi; WAKATAKE Takanori; ISHIDA Juliane K; YOSHIDA Satoko; ICHIHASHI Yasunori; SHIRASU Ken
日本植物生理学会年会(Web), 巻:60th, 2019年, [筆頭著者]
J-Global ID:201902282247993674 - 寄生植物コシオガマにおけるsubtilaseの分類と発現動態解析
OGAWA Satoshi; WAKATAKE Takanori; YOSHIDA Satoko; ICHIHASHI Yasunori; SHIRASU Ken
日本植物生理学会年会(Web), 巻:59th, 2018年, [筆頭著者]
J-Global ID:201802249331828856 - イネのフラボノイド化合物サクラネチンの生産誘導におけるジャスモン酸とフォスファチジン酸の協調作用
石田明大; 小川哲史; 西澤洋子; 南栄一; 山根久和; 有村源一郎; 野尻秀昭; 岡田憲典
日本農芸化学会大会講演要旨集(Web), 巻:2017, 2017年
ISSN:2186-7976, J-Global ID:201702250042792762 - イネのサクラネチン生合成酵素遺伝子を制御する転写因子の探索
小川哲史; 宮本皓司; 山根久和; 野尻秀昭; 岡田憲典
日本植物生理学会年会要旨集, 巻:56th, 2015年, [筆頭著者]
J-Global ID:201502298418204255 - イネのサクラネチン生合成酵素遺伝子OsNOMTの転写を制御する因子の探索
小川哲史; 宮本皓司; 山根久和; 野尻秀昭; 岡田憲典
日本農芸化学会大会講演要旨集(Web), 巻:2015, 2015年, [筆頭著者]
ISSN:2186-7976, J-Global ID:201502255531516483 - イネのフラボノイド型ファイトアレキシン生合成酵素遺伝子の発現制御機構の解析
小川哲史; 宮本皓司; 山根久和; 野尻秀昭; 岡田憲典
植物化学調節学会第49回大会, 号:Supplement, 開始ページ:56, 2014年10月, [筆頭著者]
日本語, 会議報告等
ISSN:1346-5406, J-Global ID:201402228794551599 - Biological roles of sakuranetin, a flavonoid specialized metabolite inductively produced in rice
Satoshi Ogawa; Chie Yoshiga; Gen-ichiro Arimura; Takafumi Shimizu; Koji Miyamoto; Koichi Hamada; Yoko Nishizawa; Eiichi Minami; Hisakazu Yamane; Hideaki Nojiri; Kazunori Okada
The 41st Annual Meeting of the Plant Growth Regulation Society of America, 開始ページ:8, 2014年07月, [査読有り], [筆頭著者]
英語, 会議報告等 - イネのサクラネチン生合成酵素遺伝子OsNOMTのジャスモン酸依存的な発現機構の解析
小川哲史; 野尻秀昭; 岡田憲典
第55回日本植物生理学会, 開始ページ:PF154 (0571), 2014年03月, [筆頭著者]
日本語, 会議報告等
J-Global ID:201402221595190558 - イネにおけるサクラネチン生合成酵素遺伝子のジャスモン酸依存的な発現制御機構の解明
小川哲史; 清水崇史; 山根久和; 山根久和; 野尻秀昭; 岡田憲典
日本農芸化学会大会講演要旨集(Web), 巻:2014, 開始ページ:2C04a10, 2014年03月, [筆頭著者]
日本語, 会議報告等
ISSN:2186-7976, J-Global ID:201402232787456731 - イネの病害抵抗性を制御する転写因子OsWRKY53の標的遺伝子の同定
小川哲史; 宮本皓司; 清水崇史; 増田優花; 中条哲也; 西澤洋子; 南栄一; 山根久和; 野尻秀昭; 岡田憲典
日本農芸化学会大会講演要旨集(Web), 巻:2013, 開始ページ:2A44p04, 2013年03月, [筆頭著者]
日本語, 会議報告等
ISSN:2186-7976, J-Global ID:201302296458587141 - イネの病害抵抗性を制御する転写因子OsWRKY53の相互作用因子の探索と機能解析
小川哲史; 宮本皓司; 清水崇史; 増田優花; 中条哲也; 西澤洋子; 南栄一; 野尻秀昭; 山根久和; 岡田憲典
植物の生長調節, 巻:47, 号:Supplement, 開始ページ:P92, 2012年10月, [筆頭著者]
日本語, 会議報告等
ISSN:1346-5406, J-Global ID:201202292509203316 - Molecular mechanisms for disease resistance in rice that is regulated by the transcriptional activator OsWRKY53
Satoshi Ogawa; Koji Miyamoto; Takafumi Shimizu; Yuka Masuda; Tetsuya Chujo; Yoko Nishizawa; Eiichi Minami; Hideaki Nojiri; Hisakazu Yamane; Kazunori Okada
XV International Congress on Molecular Plant-Microbe Interactions, 開始ページ:PS13-485, 2012年07月, [査読有り], [筆頭著者]
英語, 会議報告等 - イネの病害抵抗性を制御する転写因子OsWRKY53の機能発現機構の解析
小川哲史; 清水崇史; 宮本皓司; 西澤洋子; 南栄一; 増田優花; 中条哲也; 岡田憲典; 野尻秀昭; 山根久和
日本農芸化学会大会講演要旨集(Web), 巻:2012, 開始ページ:3A30a01, 2012年03月, [筆頭著者]
日本語, 会議報告等
ISSN:2186-7976, J-Global ID:201302273439890283 - イネの病害抵抗性に関与する転写因子OsWRKY53の翻訳後修飾機構の解明
増田優花; 小川哲史; 中条哲也; 宮本皓司; 岡田憲典; 加星光子; 高橋章; 西澤洋子; 南栄一; 野尻秀昭; 山根久和
日本農芸化学会大会講演要旨集, 巻:2011, 開始ページ:3A35p14, 2011年03月
J-Global ID:201102222746394201
- 植物の生長調節 58 (2)
小川哲史; 白須賢, [分担執筆], ストリゴラクトンの新奇生理機能 - ハマウツボ科寄生植物における宿主屈性の誘導
一般社団法人 植物化学調節学会, 2023年12月
共同研究・競争的資金等ID:35768353 - 月刊アグリバイオ 7 (11)
小川哲史, [分担執筆], 特集「寄生植物の宿主植物への接近メカニズム」
北隆館, 2023年09月 - 生物の科学 遺伝 74(5)
小川哲史; 白須賢, [分担執筆], 植物がハイジャックされたとき -寄生植物の感染機構
エヌ・ティー・エス, 2020年09月
- チャンスは一瞬。必ず掴もう!
小川哲史
第42回植物生理若手の会, 2025年03月, [招待有り]
2025年03月 - 2025年03月, 日本語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:47156465 - 植物の生理現象を調節する低分子化合物-日米で学んだこと
小川哲史
第6回植物生長調節物質若手研究会, 2024年09月, [招待有り]
2024年09月 - 2024年09月, 日本語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:47156465 - ハマウツボ科寄生植物が宿主に接近する分子メカニズム
小川哲史
東北大学セミナー, 2023年11月, [招待有り]
英語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:35768353 - Molecular basis for host tropism in Orobanchaceae parasitic plants
Satoshi Ogawa
KU-ICR Seminar, 2023年11月, [招待有り]
英語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:35768353 - ハマウツボ科寄生植物における宿主への接近メカニズム
小川 哲史
明治大学 植物科学セミナー, 2023年04月, [招待有り]
日本語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:35768353 - Molecular basis underlying host tropism in Orobanchaceae parasitic plants
Satoshi Ogawa
ITbM-GTR Seminar, 2023年04月, [招待有り]
英語, 公開講演,セミナー,チュートリアル,講習,講義等
共同研究・競争的資金等ID:35768353 - Orobanchaceae parasitic plants use strigolactones as chemoattractants for host tropism
Satoshi Ogawa
The International Parasitic Plant Society Online Seminar Series #11, 2022年04月, [招待有り]
英語, 公開講演,セミナー,チュートリアル,講習,講義等
- 2019年06月 - 現在
- 2014年01月 - 現在, 日本植物生理学会
- 2012年10月 - 現在, 植物化学調節学会
- 2010年12月 - 現在, 日本農芸化学会
- 2022年04月 - 2023年03月
- KAI2 ligandシグナルが関与する植物ホルモン間クロストークの解明
日本学術振興会, 科学研究費助成事業, 基盤研究(C), 2025年04月 - 2028年03月
小川 哲史, 京都大学, 研究代表者
配分額(総額):6370000, 配分額(直接経費):4900000, 配分額(間接経費):1470000
課題番号:25K09038 - Identification of biosynthetic genes for a novel signaling molecule that modulates plant development
Institute for Chemical Research, Kyoto University, International Collaborative Research Program of Institute for Chemical Research, International Collaborative Research, 2025年04月 - 2026年03月
David C. Nelson; Shinjiro Yamaguchi; Qianfan Xu; Kiyoshi Mashiguchi; Satoshi Ogawa, Kyoto University, 研究分担者
課題番号:2025-107 - Identification of biosynthetic genes for a novel signaling molecule that modulates plant development
2024年04月 - 2025年03月
研究分担者
課題番号:2024-102
MISC ID:49430771, 講演・口頭発表等ID:49430596 - 寄生植物-宿主間と植物-菌根菌間における他者認識の分子機構
日本学術振興会, 科学研究費助成事業 特別研究員奨励費, 特別研究員奨励費, 2021年04月 - 2024年03月
小川 哲史, 国立研究開発法人理化学研究所, 研究代表者
配分額(総額):4810000, 配分額(直接経費):3700000, 配分額(間接経費):1110000
本研究では、植物が菌根菌を認識するメカニズムと寄生植物が宿主植物を認識するメカニズムにおける共通点を分子レベルで解明することを目指している。今年度は寄生植物が宿主植物を認識し接近する屈性のメカニズムに着目し、主にハマウツボ科の半寄生植物であるコシオガマを材料として研究を進めた。
研究開始段階までにコシオガマにおける屈性を評価するシステム立ち上げ、宿主の根から放出される植物ホルモンのストリゴラクトン(SL)に対してコシオガマの根が屈性を示すことを明らかにしてきた。今年度はイネに対する寄生実験を行い、コシオガマはSL生合成変異株よりも野生型のイネに対して屈性を示す傾向があることを示した。またコシオガマ以外の植物を用いた実験により、SLに対する屈性がハマウツボ科寄生植物のストライガでも見られる一方で非寄生植物では見られないことを示し、SLへの屈性がハマウツボ科寄生植物に特異的な戦略である可能性を提唱した。コシオガマの根ではSLの左右非対称な認識が行われるが、培地中にアンモニウムイオンが存在するとSLの認識はそのままに屈性が妨げられることを示した。一方SLの左右非対称な認識と同様の局在パターンを示すオーキシン輸送体PIN2について、アンモニウムの存在下では局在が見られなくなることを示し、SLの認識からPIN2の蓄積に至るまでのシグナル伝達がアンモニウムイオンにより阻害されることを示唆した。さらに、外部のSLを認識する受容体の候補を同定し、受容体の機能を阻害すると屈性が抑制されることを明らかにした。
以上のように、ハマウツボ科寄生植物はSLを受容することで宿主植物を認識し屈性を示すこと、PIN2の局在を介したSLへの屈性はアンモニウムイオンにより抑制されることを明らかにした。
課題番号:21J00718
論文ID:49275740, MISC ID:45863776, 書籍等出版物ID:44529284, 講演・口頭発表等ID:44143625, 受賞ID:41802386