業績情報

• Physical constraints on the positions and dimensions of the zebrafish swim bladder by surrounding bones.　　　　　　　　　　　　　　　
  Koumi Satoh; Akiteru Maeno; Urara Adachi; Mizuki Ishizaka; Kazuya Yamada; Rina Koita; Hidemichi Nakazawa; Sae Oikawa; Renka Fujii; Hiroyuki Furudate; Akinori Kawamura
  Journal of anatomy, 2024年11月, ［査読有り］, ［国際誌］
  Precise regulation of organ size and position is crucial for optimal organ function. Since the swim bladder is primarily responsible for buoyancy in teleosts, early development and subsequent inflation of the swim bladder should be appropriately controlled with the body growth. However, the underlying mechanism remains unclear. In this study, we show that the size and position of the swim bladder are physically constrained by the surrounding bones in zebrafish. Non-invasive micro-CT scanning revealed that the anterior edge of the swim bladder is largely attached to the os suspensorium, which is an ossicle extending medioventrally from the 4th centrum. Additionally, we observed that hoxc6a mutants, which lack the os suspensorium, exhibited an anterior projection of the swim bladder beyond the 4th vertebra. During the swim bladder development, we found that the counterclockwise rotation of the os suspensorium correlates with posterior regression of the swim bladder, suggesting that the os suspensorium pushes the swim bladder posteriorly into its proper position. Furthermore, our results revealed a close association between the posterior region of the swim bladder and the pleural ribs. In hoxaa cluster mutants with additional ribs, the swim bladder expanded posteriorly, accompanied by an enlarged body cavity. Taken together, our results demonstrate the importance of the surrounding bones in the robust regulation of swim bladder size and position in zebrafish.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1111/joa.14179
  DOI ID：10.1111/joa.14179, PubMed ID：39556020
• Teleost Hox code defines regional identities competent for the formation of dorsal and anal fins.　　　　　　　　　　　　　　　
  Urara Adachi; Rina Koita; Akira Seto; Akiteru Maeno; Atsuki Ishizu; Sae Oikawa; Taisei Tani; Mizuki Ishizaka; Kazuya Yamada; Koumi Satoh; Hidemichi Nakazawa; Hiroyuki Furudate; Koichi Kawakami; Norimasa Iwanami; Masaru Matsuda; Akinori Kawamura
  Proceedings of the National Academy of Sciences of the United States of America, 巻：121, 号：25, 開始ページ：e2403809121, 2024年06月, ［査読有り］, ［国際誌］
  The dorsal and anal fins can vary widely in position and length along the anterior-posterior axis in teleost fishes. However, the molecular mechanisms underlying the diversification of these fins remain unknown. Here, we used genetic approaches in zebrafish and medaka, in which the relative positions of the dorsal and anal fins are opposite, to demonstrate the crucial role of hox genes in the patterning of the teleost posterior body, including the dorsal and anal fins. By the CRISPR-Cas9-induced frameshift mutations and positional cloning of spontaneous dorsalfinless medaka, we show that various hox mutants exhibit the absence of dorsal or anal fins, or a stepwise posterior extension of these fins, with vertebral abnormalities. Our results indicate that multiple hox genes, primarily from hoxc-related clusters, encompass the regions responsible for the dorsal and anal fin formation along the anterior-posterior axis. These results further suggest that shifts in the anterior boundaries of hox expression which vary among fish species, lead to diversification in the position and size of the dorsal and anal fins, similar to how modulations in Hox expression can alter the number of anatomically distinct vertebrae in tetrapods. Furthermore, we show that hox genes responsible for dorsal fin formation are different between zebrafish and medaka. Our results suggest that a novel mechanism has occurred during teleost evolution, in which the gene network responsible for fin formation might have switched to the regulation downstream of other hox genes, leading to the remarkable diversity in the dorsal fin position.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1073/pnas.2403809121
  DOI ID：10.1073/pnas.2403809121, PubMed ID：38861596, PubMed Central ID：PMC11194558
• Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens.　　　　　　　　　　　　　　　
  Yoshihara R, Mitomi Y, Okada M, Shibata H, Tanokami M, Nakajima Y, Inui H, Oono Y, Furudate H, Tanaka S
  Genes Genet Syst, 巻：95(4), 開始ページ：173, 終了ページ：182, 2020年08月, ［査読有り］
• Contribution of Nucleus Accumbens Core (AcbC) to Behavior Control during a Learned Resting Period: Introduction of a Novel Task and Lesion Experiments.　　　　　　　　　　　　　　　
  Sato C, Hoshino M, Ikumi N, Oba K, Koike A, Shouno O, Sekiguchi T, Kobayashi T, Machida T, Matsumoto G, Furudate H and Kimura T
  PLoS One, 巻：9, 号：4, 開始ページ：e95941, 2014年04月, ［査読有り］
  In recent years, the study of resting state neural activity has received much attention. To better understand the roles of different brain regions in the regulation of behavioral activity in an arousing or a resting period, we developed a novel behavioral paradigm (8-arm food-foraging task; 8-arm FFT) using the radial 8-arm maze and examined how AcbC lesions affect behavioral execution and learning. Repetitive training on the 8-arm FFT facilitated motivation of normal rats to run quickly to the arm tips and to the center platform before the last-reward collection. Importantly, just after this point and before confirmation of no reward at the next arm traverse, locomotor activity decreased. This indicates that well-trained rats can predict the absence of the reward at the end of food seeking and then start another behavior, namely planned resting. Lesions of the AcbC after training selectively impaired this reduction of locomotor activity after the last-reward collection without changing activity levels before the last-reward collection. Analysis of arm-selection patterns in the lesioned animals suggests little influence of the lesion in the ability to predict the reward absence. AcbC lesions did not change exploratory locomotor activity in an open-field test in which there were no rewards. This suggests that the AcbC controls the activity level of planned resting behavior shaped by the 8-arm FFT. Rats receiving training after AcbC lesioning showed a reduction in motivation for reward seeking. Thus, the AcbC also plays important roles not only in controlling the activity level after the last-reward collection but also in motivational learning for setting the activity level of reward-seeking behavior.
  PUBLIC LIBRARY SCIENCE, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1371/journal.pone.0095941
  DOI ID：10.1371/journal.pone.0095941, ISSN：1932-6203, Web of Science ID：WOS:000346241800019
• Internal representation and memory formation of odor preference based on oscillatory activities in a terrestrial slug.　　　　　　　　　　　　　　　
  Sekiguchi, T., Furudate, H. and Kimura, T.
  Learning and Memory, 巻：17, 号：8, 開始ページ：372, 終了ページ：80, 2010年08月, ［査読有り］, ［国際誌］
  The terrestrial slug Limax exhibits a highly developed ability to learn odors with a small nervous system. When a fluorescent dye, Lucifer Yellow (LY), is injected into the slug's body cavity after odor-taste associative conditioning, a group of neurons in the procerebral (PC) lobe, an olfactory center of the slug, is labeled by LY. We examined the relationships between conditioning strategies and LY labeling. The positions of LY-labeled neurons in the PC lobe after appetitive conditioning were more apical than those after aversive conditioning and did not depend on the conditioned odor, suggesting that the biological value of odors affected the position of LY-labeled neural clusters. A simple computational model consisting of two layers of oscillators with electrical synaptic interaction was constructed based on physiological features of the PC lobe, and showed that the oscillatory phase difference between the layers contributed to determination of the positions of LY-labeled neurons, suggesting that phase difference in oscillatory activity plays a role in the association of odors and the preference for them.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1101/lm.1867110
  DOI ID：10.1101/lm.1867110, PubMed ID：20663752
• GABA(A) receptor-mediated acceleration of aging-associated memory decline in APP/PS1 mice and its pharmacological treatment by picrotoxin.　　　　　　　　　　　　　　　
  Yoshiike Y, Kimura T, Yamashita S, Furudate H, Mizoroki T, Murayama M, Takashima A.
  PLoS ONE, 巻：3, 号：8, 開始ページ：e3029, 2008年08月, ［査読有り］
  Advanced age and mutations in the genes encoding amyloid precursor protein (APP) and presenilin (PS1) are two serious risk factors for Alzheimer's disease (AD). Finding common pathogenic changes originating from these risks may lead to a new therapeutic strategy. We observed a decline in memory performance and reduction in hippocampal long-term potentiation (LTP) in both mature adult (9-15 months) transgenic APP/PS1 mice and old (19-25 months) non-transgenic (nonTg) mice. By contrast, in the presence of bicuculline, a GABA(A) receptor antagonist, LTP in adult APP/PS1 mice and old nonTg mice was larger than that in adult nonTg mice. The increased LTP levels in bicuculline-treated slices suggested that GABA(A) receptor-mediated inhibition in adult APP/PS1 and old nonTg mice was upregulated. Assuming that enhanced inhibition of LTP mediates memory decline in APP/PS1 mice, we rescued memory deficits in adult APP/PS1 mice by treating them with another GABA(A) receptor antagonist, picrotoxin (PTX), at a non-epileptic dose for 10 days. Among the saline vehicle-treated groups, substantially higher levels of synaptic proteins such as GABA(A) receptor alpha 1 subunit, PSD95, and NR2B were observed in APP/PS1 mice than in nonTg control mice. This difference was insignificant among PTX-treated groups, suggesting that memory decline in APP/PS1 mice may result from changes in synaptic protein levels through homeostatic mechanisms. Several independent studies reported previously in aged rodents both an increased level of GABA(A) receptor alpha 1 subunit and improvement of cognitive functions by long term GABA(A) receptor antagonist treatment. Therefore, reduced LTP linked to enhanced GABA(A) receptor-mediated inhibition may be triggered by aging and may be accelerated by familial AD-linked gene products like A beta and mutant PS1, leading to cognitive decline that is pharmacologically treatable at least at this stage of disease progression in mice.
  PUBLIC LIBRARY SCIENCE, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1371/journal.pone.0003029
  DOI ID：10.1371/journal.pone.0003029, ISSN：1932-6203, Web of Science ID：WOS:000264426800007
• Responses of nonspiking giant interneurons to leg movements in the crayfish, with special reference to multisensory interaction in the equibrium system.　　　　　　　　　　　　　　　
  H. Furudate; Y. Okada; T. Yamaguchi
  Journal of Comparative Physiology A, 巻：179, 開始ページ：635, 終了ページ：643, 1996年, ［査読有り］
  J. Comp. Physiol. A
• Multimodal responses of the nonspiking giant interneurons in the brain of the crayfish Procambarus clarkii.　　　　　　　　　　　　　　　
  Y Okada; H Furudate; T Yamaguchi
  Journal of Comparative Physiology A, 巻：174, 開始ページ：411, 終了ページ：419, 1994年, ［査読有り］
  J. Comp. Physiol. A

• 富永佳也編「昆虫の脳を探る」　　　　　　　　　　　　　　　
  共著
  共立出版, 1995年

• 成長遅延症マウスにおける免疫能および造血能の解析　　　　　　　　　　　　　　　
  日本動物学会関東支部大会発表演者要旨集, 2009年
• 成長遅延症マウスにおける免疫能および造血能の解析　　　　　　　　　　　　　　　
  日本動物学会関東支部大会発表演者要旨集, 2009年
• ラットの行動モード切り替えにともなう海馬場所ニューロン活動の変化　　　　　　　　　　　　　　　
  日本動物学会79回大会予稿集, 2008年
• アルツハイマー病モデルネズミのGABA依存的認知障害とピクロトキシンによるその改善　　　　　　　　　　　　　　　
  第31回日本神経科学会大会予稿集, 2008年
• ナメクジにおけるにおい嗜好性記憶の内部表現　　　　　　　　　　　　　　　
  第31回日本神経科学会大会予稿集, 2008年
• ラットの行動モード変化にともなう海馬ニューロン活動の特異的発現　　　　　　　　　　　　　　　
  日本動物学会関東支部大会第60回大会, 2008年
• ラットの行動モード切り替えにともなう海馬場所ニューロン活動の変化　　　　　　　　　　　　　　　
  日本動物学会79回大会予稿集, 2008年
• アルツハイマー病モデルネズミのGABA依存的認知障害とピクロトキシンによるその改善　　　　　　　　　　　　　　　
  第31回日本神経科学会大会予稿集, 2008年
• ナメクジにおけるにおい嗜好性記憶の内部表現　　　　　　　　　　　　　　　
  第31回日本神経科学会大会予稿集, 2008年
• ラットの行動モード変化にともなう海馬ニューロン活動の特異的発現　　　　　　　　　　　　　　　
  日本動物学会関東支部大会第60回大会, 2008年
• マウス腹内側核におけるERαとc-Fosの発現に対する甲状腺ホルモンの効果　　　　　　　　　　　　　　　
  社団法人日本動物学会第78回大会予稿集, 2007年
• アルツハイマー病モデルネズミの老化促進機構におけるGABA活性の解析　　　　　　　　　　　　　　　
  第30回日本神経科学大会, 2007年
• マウス腹内側核におけるERαとc-Fosの発現に対する甲状腺ホルモンの効果　　　　　　　　　　　　　　　
  社団法人日本動物学会第78回大会予稿集, 2007年
• アルツハイマー病モデルネズミの老化促進機構におけるGABA活性の解析　　　　　　　　　　　　　　　
  第30回日本神経科学大会, 2007年
• Restricted brain lesions affect the filial preferences in visually imprinted ducklings.　　　　　　　　　　　　　　　
  Society for Neuroscience 36th Annual Meeting (SFN-2006), 2006年
• 行動モード変化にともなう海馬ニューロン活動の特異的表現　　　　　　　　　　　　　　　
  社団法人日本動物学会第77回大会予稿集, 2006年
• 学習された行動モードに対する側坐核コア領域破壊の影響　　　　　　　　　　　　　　　
  社団法人日本動物学会第77回大会予稿集, 2006年
• Restricted brain lesions affect the filial preferences in visually imprinted ducklings.　　　　　　　　　　　　　　　
  Society for Neuroscience 36th Annual Meeting (SFN-2006), 2006年
• 行動モード変化にともなう海馬ニューロン活動の特異的表現　　　　　　　　　　　　　　　
  社団法人日本動物学会第77回大会予稿集, 2006年
• 学習された行動モードに対する側坐核コア領域破壊の影響　　　　　　　　　　　　　　　
  社団法人日本動物学会第77回大会予稿集, 2006年
• Neural Activities in the rat nucleus accumbens related to foraging behavior in an eight-arm maze task.　　　　　　　　　　　　　　　
  Society of Neuroscience 35th Annual Meeting, 2005年
• Neural Activities in the rat nucleus accumbens related to foraging behavior in an eight-arm maze task.　　　　　　　　　　　　　　　
  Society of Neuroscience 35th Annual Meeting, 2005年
• Neuronal activities related to filial imprinting in the forebrain area of duckling.　　　　　　　　　　　　　　　
  Society of Neuroscience 34th Annual Meeting, 2004年
• Neural activity in the nucleus accumbens core represents a global pattern of reward directed behavior in the rat.　　　　　　　　　　　　　　　
  Society of Neuroscience 34th Annual Meeting, 2004年
• 側坐核ニューロンにおける行動モード表現　　　　　　　　　　　　　　　
  社団法人日本動物学会第75回大会予稿集, 2004年
• Neuronal activities related to filial imprinting in the forebrain area of duckling.　　　　　　　　　　　　　　　
  Society of Neuroscience 34th Annual Meeting, 2004年
• Neural activity in the nucleus accumbens core represents a global pattern of reward directed behavior in the rat.　　　　　　　　　　　　　　　
  Society of Neuroscience 34th Annual Meeting, 2004年
• 側坐核ニューロンにおける行動モード表現　　　　　　　　　　　　　　　
  社団法人日本動物学会第75回大会予稿集, 2004年

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  競争的資金
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  競争的資金