Individual and combined effects of ionophore antibiotics monensin and lasalocid on growth, oxidative stress and antioxidant responses of Microcystis aeruginosaBehen Manawadu; Mudalige Don Hiranya Jayasanka Senavirathna; Takeshi Fujino
Water Science & Technology,
巻：90,
号：9,
開始ページ：2585,
終了ページ：2601, 2024年10月,
［査読有り］,
［責任著者］ABSTRACT
To assess the impact of monensin, lasalocid, and a 1:1 (w/w) mixture of monensin and lasalocid on the formation of Microcystis aeruginosa-based harmful algal blooms (HABs), laboratory experiments were conducted [temperature 20 ± 1°C, illumination PAR 30 ± 4 μmol/m2 ·s (12-h light/dark cycle), growth medium BG-11] at concentrations of 100, 200, 500, 1,000, and 2,000 μg/L. Measurements included optical density, cell protein, chlorophyll ‘a’ (Chl. a) content, oxidative stress, catalase activity (CAT), and guaiacol peroxidase activity (GPX). Monensin treatment showed a dose-dependent positive effect on M. aeruginosa growth at concentrations ≤500 μg/L and a gradual dose-dependent growth inhibition at concentrations of 1,000 and 2,000 μg/L. The results indicated that monensin has a significant positive effect on the formation of HABs by M. aeruginosa. Lasalocid treatment showed a growth reduction of M. aeruginosa at concentrations ≤500 μg/L and a growth increase at higher concentrations (1,000 and 2,000 μg/L). The 1:1 (w/w) mixture test showed an intermediate response, as indicated by the individual treatments signifying the potential interactive effects of these antibiotics on M. aeruginosa. Furthermore, alterations in Chl. ‘a’, oxidative stress, CAT, and GPX measurements provided evidence of the impact of these antibiotics on the existence of M. aeruginosa.
IWA Publishing, 研究論文（学術雑誌）
DOI：https://doi.org/10.2166/wst.2024.356DOI ID：10.2166/wst.2024.356,
ISSN：0273-1223,
eISSN：1996-9732 Assessing the biochemical and genotoxic effects of low intensity 2.45GHz microwave exposure on Arabidopsis thaliana plantsMudalige Don Hiranya Jayasanka Senavirathna; Zumulati Maimaiti
Electromagnetic Biology and Medicine,
巻：43,
号：4,
開始ページ：303,
終了ページ：311, 2024年10月,
［査読有り］,
［筆頭著者, 責任著者］Informa UK Limited, 研究論文（学術雑誌）
DOI：https://doi.org/10.1080/15368378.2024.2411629DOI ID：10.1080/15368378.2024.2411629,
ISSN：1536-8378,
eISSN：1536-8386 Urban Planning with Rational Green Infrastructure Placement Using a Critical Area Detection MethodHerath Mudiyanselage Malhamige Sonali Dinesha Herath; Takeshi Fujino; Mudalige Don Hiranya Jayasanka Senavirathna
Geomatics,
巻：4,
号：3,
開始ページ：253,
終了ページ：270, 2024年07月,
［査読有り］,
［最終著者］In an era of intense urban development and climate extremes, green infrastructure (GI) has become crucial for creating sustainable, livable, and resilient cities. However, the efficacy of GI is frequently undermined by haphazard implementation and resource misallocation that disregards appropriate spatial scales. This study develops a geographic information system (GIS)-based critical area detection model (CADM) to identify priority areas for the strategic placement of GI, incorporating four main indices—spatial form, green cover, gray cover, and land use change—and utilizing the digital elevation model (DEM), normalized difference vegetation index (NDVI), urban density index (UDI), and up-to-date land use data. By employing the developed method, the study successfully locates priority zones for GI implementation in Saitama City, Japan, effectively pinpointing areas that require immediate attention. This approach not only guarantees efficient resource allocation and maximizes the multifunctional benefits of GI but also highlights the importance of a flexible, all-encompassing GI network to address urbanization and environmental challenges. The findings offer policymakers a powerful tool with which to optimize GI placement, enhancing urban resilience and supporting sustainable development.
MDPI AG, 研究論文（学術雑誌）
DOI：https://doi.org/10.3390/geomatics4030014DOI ID：10.3390/geomatics4030014,
eISSN：2673-7418 The Vertical Migration Dynamic of Microcystis aeruginosa in a Water ColumnHongyu Yan; Mudalige Don Hiranya Jayasanka Senavirathna; Takeshi Fujino
Journal of Applied Phycology,
巻：36,
号：4,
開始ページ：1921,
終了ページ：1937, 2024年04月,
［査読有り］,
［責任著者］Springer Science and Business Media LLC, 研究論文（学術雑誌）
DOI：https://doi.org/10.1007/s10811-024-03241-xDOI ID：10.1007/s10811-024-03241-x,
ISSN：0921-8971,
eISSN：1573-5176 Growth and physiological responses of Myriophyllum sp. “Roraima” growing in co-cultivation with Microcystis aeruginosa under the influence of microplastics Zhaozhi Liu; Mudalige Don Hiranya Jayasanka Senavirathna; Takeshi Fujino
Environmental and Experimental Botany,
巻：222,
開始ページ：105745,
終了ページ：105745, 2024年03月,
［査読有り］,
［責任著者］Elsevier BV, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.envexpbot.2024.105745DOI ID：10.1016/j.envexpbot.2024.105745,
ISSN：0098-8472 Flow velocity and light intensity combination is important for Microcystis aeruginosa physical suppressionSenavirathna, M.D.H.J., Yan, H.
Water Environment Researchs,
巻：96,
号：2,
開始ページ：Article e10991, 2024年01月,
［査読有り］,
［責任著者］Abstract
The cyanobacterial response to flow velocity or light intensity deviates from the combined effect of both factors. The responses of Microcystis aeruginosa to different combinations of flow velocities and light intensities were tested. Growth (OD
730 and protein), stress (catalase, ascorbate peroxidase, and glutathione peroxidase), and photosynthetic ability (chlorophyll‐a and fluorescence) parameters of M. aeruginosa were measured to evaluate the effects of different combinations. Exposure to different flow velocity–light combinations significantly affected the growth and physiology of M. aeruginosa. Flow velocities of 0.4 m s
−1 showed a prominent influence on most of the measured parameters compared with no flow velocity or higher flow velocity conditions. The 1.2‐m s
−1 flow velocity and high light intensity (1200 μmol m
−2 s
−1) exposure caused a significant elevation in oxidative stress. Lower velocities are beneficial for M. aeruginosa at light stress, whereas extreme velocities are adverse and elevate the stress. Two categories of light–velocity combinations were identified as preferred and extreme categories, depending on whether they suppressed or supported M. aeruginosa growth. In controlling cyanobacteria blooms using flow or high‐intensity light, it is imperative to consider the interaction of these two factors, as their combined effects can significantly vary the stress levels in cyanobacteria. A new system, designed to minimize mechanical damage on M. aeruginosa, was used to generate flow velocities. Additionally, the combined effects of flow velocities and light intensities have been considered for the first time.
Practitioner Points
Flow velocity can influence the effect of light on Microcystis aeruginosa.
High light exposure effect on Microcystis aeruginosa can be reduced by low flow velocity.
High flow velocity and high light exposure increase the stress on Microcystis aeruginosa.
Different light intensities and flow velocity combinations changed Microcystis aeruginosa stress physiology.
Wiley, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/wer.10991DOI ID：10.1002/wer.10991,
ISSN：1061-4303,
eISSN：1554-7531 Egeria densa organic extracts: an eco-friendly approach to suppress Microcystis aeruginosa growth through allelopathyAshika Wijesinghe; Mudalige Don Hiranya Jayasanka Senavirathna; Takeshi Fujino
Water Science & Technology,
巻：88,
号：11,
開始ページ：2955,
終了ページ：2973, 2023年11月,
［査読有り］,
［責任著者］Abstract
Macrophytes play a significant role in shaping plankton communities by shading, controlling water turbulence, and nutrient availability, while also producing allelochemicals causing varying effects on different organisms. Many researchers have shown that when live macrophytes are present, they inhibit cyanobacteria. However, their widespread use is often limited due to numerous concerns, including invasive characteristics. This study focused on the applicability of Egeria densa extracts to suppress Microcystis aeruginosa. We employed pure water and dimethyl sulfoxide, to obtain compounds from E. densa. The results revealed that E. densa aqueous extracts stimulated M. aeruginosa growth, whereas organic extracts exhibited suppression. Specifically, at low concentrations of organics extracts (0.5 and 1 g/L), after day 4, the growth inhibition was confirmed by significantly higher (p < 0.05) stress levels shown in cells treated with low concentrations. The highest inhibition rate of 32% was observed at 0.5 g/L. However, high concentrations of organic extracts (3 and 6 g/L), showed increased growth compared with control. These results suggest that high concentrations of organic extracts from E. densa potentially suppress allelochemical-induced M. aeruginosa inhibition due to high nutrient availability. In comparison with an aqueous solvent, the use of organic solvent seems to be more effective in efficiently extracting allelochemicals from E. densa.
IWA Publishing, 研究論文（学術雑誌）
DOI：https://doi.org/10.2166/wst.2023.387DOI ID：10.2166/wst.2023.387,
ISSN：0273-1223,
eISSN：1996-9732 A Review of Emerging Scientific Discussions on Green Infrastructure (GI)‑Prospects towards Effective Use of Urban Flood Plains
Herath, H.M.M.S.D., Fujino, T., Senavirathna, M.D.H.J.
Sustainability, 巻：15, 開始ページ：1227, 2023年, ［査読有り］
Effects of rainfall on fluvial discharge and suspended sediment transport in the Central Himalayan region, Nepal
Baniya, M.B., Asaeda, T., Fujino, T., Talchabhadel, R., Baniya, R., Shivaram, K. C., Sharma, N. R., Senavirathna M.D.H.J.
Theoretical and Applied Climatology, 巻：155, 開始ページ：1553, 終了ページ：1572, 2023年
Inhibitory Effect of Aqueous Extracts from Egeria densa Planch. on Cyanobacteria Microcystis aeruginosa (Kützing) Lemmer-mann Growth Wijesinghe, N.A., Senavirathna, M.D.H.J., Fujino, T.,
Hydrobiology,
巻：2,
開始ページ：491,
終了ページ：506, 2023年,
［査読有り］,
［責任著者］DOI：https://doi.org/10.3390/hydrobiology2030033DOI ID：10.3390/hydrobiology2030033 Microwave exposure added characteristics to the wounding-induced variation potential of Aloe arborescens leaves
Senavirathna, M.D.H.J.
Sensors International, 巻：4, 開始ページ：Article 100241, 2023年
Recoverability of Microcystis aeruginosa and Pseudanabaena foetida Exposed to a Year-Long Dark Treatment
Yan, H., Senavirathna, M.D.H.J.
Microorganisms, 巻：11, 開始ページ：Article 2760, 2023年
Root Adsorption of Microplastic Particles Affects the Submerged Freshwater Macrophyte Egeria densa
Mudalige Don Hiranya Jayasanka Senavirathna, Liu Zhaozhi, Takeshi Fujino
Water, Air, & Soil Pollution, 巻：233, 号：80, 2022年
Short-duration exposure of 3-µm polystyrene microplastics affected morphology and physiology of watermilfoil (sp. roraima)
Mudalige Don Hiranya Jayasanka Senavirathna, Liu Zhaozhi, Takeshi Fujino
Environmental Science and Pollution Research, 巻：29, 開始ページ：34475, 終了ページ：34485, 2022年
Temporal variation of 2‐MIB and Geosmin production by Pseudanabaena galeata and Phormidium ambiguum exposed to high intensity light
Mudalige Don Hiranya Jayasanka Senavirathna, Mohoppu Arachchillage Dasun Dineka Jayasekara
Water Environment Research, 巻：95, 号：1, 開始ページ：e10834, 2022年, ［査読有り］
Egeria densa Allelopathy on Microcystis aeruginosa Under Different Light Intensities and Preliminary Insight into Inter-Parameter Relationships
Senavirathna, M.D.H.J., Muhetaer, G., Atapaththu, K.S.S., Fujino, T.
Water Air and soil pollution, 巻：232, 号：135, 2021年
Stress and Recovery Responses of Microcystis aeruginosa Exposed to Extreme Light for Different Durations
Senavirathna, M.D.H.J., Muhetaer, G., Yan, H., Aihemaiti, B., Fujino, T.
Water Air and soil pollution, 巻：229, 号：232, 開始ページ：253, 2021年
Visualization and Quantification of the Impact of Humic Acid on Zinc Accumulation in Aquatic Plants Using a Low-Molecular-Weight Fluorescent Probe
Meetiyagoda, T.A.O.K., Fedilah, K., Hagimori, M., Senavirathna, M.D.H.J., Fujino, T.
Journal of Water and Environment Technology, 巻：19, 開始ページ：49, 終了ページ：63, 2021年
Effects of short-term exposure to different salinity levels on Myriophyllum spicatum and Ceratophyllum demersum and suitability of biomarkers to evaluate macrophyte responses to salinity stress
Senavirathna, M.D.H.J., Wijesinghe, N.A., Zhaozhi, L., Fujino, T.
International Journal of Limnology, 巻：56, 号：Article 23, 2020年
Oxidative stress and antioxidant responses of Phormidium ambiguum and Microcystis aeruginosa, under diurnally varying light conditions
Muhetaer, G., Senavirathna, M.D.H. J., Fujino, T.
Microorganisms, 巻：8, 号：6, 2020年
Evaluation of habitat preferences of invasive macrophyte Egeria densa in different channel slopes using hydrogen peroxide as an indicator
Asaeda, T., Senavirathna M.D.H. J., Krishna, L.V.
Frontiers in Plant Science, 巻：11, 号：Article 422, 2020年
Effects of light intensity and exposure period on the growth and stress responses of two cyanobacteria species: Pseudanabaena galeata and Microcystis aeruginosa
Muhetaer, G., Asaeda, T., Senavirathna M.D.H.J., Baniya, M.B., Abeynayaka, H.D.L., Rashid, M.R., Yan, H.
Water, 巻：12, 号：2, 2020年
Stimulatory effect of exposure to low power density 2.45 GHz microwaves on Arabidopsis thaliana seedlings in vitro
Senavirathna, M.D.H. J., Nagahage, I.S.P., Muhetaer, G.
Brazilian Journal of Botany, 巻：43, 開始ページ：459, 終了ページ：467, 2020年
Allelopathic influence of low concentration Microcystis aeruginosa on Egeria densa under different light intensities
Senavirathna, M.D.H.J., Muhetaer, G, Zhaozhi, L., Fujino, T
Chemistry and Ecology, 巻：36, 号：10, 開始ページ：903, 終了ページ：921, 2020年
Riparian vegetation classification using the dynamic riparian vegetation model
Asaeda, T., Nallaperuma, B., Baniya, M.B., Senavirathna, M.D.H.J.
Proceedings 38th IAHR World Congress, 巻：1-1, 2019年
Mechanism of riparian vegetation growth and sediment transport interaction in floodplain: a dynamic riparian vegetation model (DRIPVEM) approach
Baniya, M.B., Asaeda, T., Fujino, T., Senavirathna M.D.H.J., Muhetaer, G., Li, J.
Water, 巻：12-2, 2019年
Hydraulic parameters for sediment transport and prediction of suspended sediment for Kali Gandaki river basin, Himalaya, Nepal.
Baniya, M.B., Asaeda, T., Shivaram, K.C., Senavirathna M.D.H.J.
Water, 巻：11-6, 2019年
THE ELECTRICAL POTENTIAL OF NICOTIANA BENTHAMIANA AFFECTED BY MICROWAVE EXPOSURE
Senavirathna, M.D.H.J., Asaeda, T.
Plant Functioning Under Environmental Stress, 巻：11, 開始ページ：42, 終了ページ：47, 2018年
Microwave radiation alters burn injury-evoked electric potential in Nicotiana benthamiana
Senavirathna, M.D.H.J., Asaeda, T.
Plant Signaling and Behavior, 巻：13, 開始ページ：5, 2018年
Microwave radiation alters burn injury-evoked electric potential in Nicotiana benthamiana
Senavirathna, M.D.H.J., Asaeda, T.
Plant Signaling and Behavior, 巻：13, 開始ページ：5, 2018年
Application of Hydrogen Peroxide as an Environmental Stress Indicator for Vegetation Management
Asaeda, T., Senavirathna, M.D.H.J,, Xia, L., Barnueo, A.
Engineering, 巻：4-5, 開始ページ：610, 終了ページ：616, 2018年
Impact of regulated water levels on willows (Salix subfragilis) at a flood-control dam, and the use of hydrogen peroxide as an indicator of environmental stress
Asaeda, T., Senavirathna, M.D.H.J, krishnaNaoya, L.V., Yoshida, N.
Ecological Engineering, 巻：127, 開始ページ：96, 終了ページ：102, 2018年
Microwave radiation alters burn injury-evoked electric potential in Nicotiana benthamiana
Senavirathna, M.D.H.J., Asaeda, T.
Plant Signaling and Behavior, 巻：13, 号：6, 開始ページ：e1486145, 2018年
Microwaves affect Myriophyllum aquaticum plants differently depending on the wave polarization
Senavirathna, M.D.H.J., Asaeda, T.
Biologia Plantarum, 巻：61-2, 開始ページ：378, 終了ページ：384, 2017年
Nanometer-scale elongation rate fluctuations in the Myriophyllum aquaticum (Parrot feather) stem were altered by radio-frequency electromagnetic radiation
Senavirathna, M.D.H.J., Asaeda, T., Thilakarathne, B.L.S., Kadono, H.
Plant Signaling and Behavior, 巻：9, 号：4, 2014年
Effect of calcium and magnesium on the growth and calcite encrustation of Chara fibrosa
Asaeda, T., Senavirathna, M.D.H.J., Kaneko, Y., Rashid, M.H.
Aquatic Botany, 巻：113, 開始ページ：100, 終了ページ：106, 2014年
The significance of microwaves in the environment and its effect on plants
Senavirathna, M.D.H.J., Asaeda, T.
Environmental Reviews, 巻：22, 号：3, 開始ページ：220, 終了ページ：228, 2014年
Short-duration exposure to radiofrequency electromagnetic radiation alters the chlorophyll fluorescence of Duckweeds (Lemna minor)
Senavirathna, M.D.H.J., Asaeda, T., Kimura, Y.
Electromagnetic Biology and Medicine, 巻：33, 号：4, 開始ページ：327, 終了ページ：334, 2013年
Radio-frequency electromagnetic radiation alters the electric potential of Parrot Feather (Myriophyllum aquaticum) plants
Senavirathna, M.D.H.J., Asaeda, T.
Biologia Plantarum, 巻：58, 号：2, 開始ページ：352, 終了ページ：362, 2013年
Modification of solar distillation unit to increase efficiency in drinking water production from saline water
Senavirathna, M.D.H.J., Weerasinghe, K.D.N., Maier, M., Rosemann, H.
Tropical Agricultural Research & Extension, 巻：12, 号：2, 開始ページ：67, 終了ページ：70, 2009年
Responses of freshwater macrophytes and cyanobacteria to microplastic exposure
Senavirathna, M.D.H.J., Liu, Z., Aihemaiti, B., Takano, H.
2023年03月, ［国際会議］
Advanced Technologies and Training and Research for Optimizing Agricultural Production
Mudalige Don Hiranya Jayasanka Senavirathna
Proceedings of the International Symposium on Agriculture and Environment 2022, 2022年06月, ［国際会議］
Riparian vegetation classification using the dynamic riparian vegetation model
Asaeda, T., Nallaperuma, B., Baniya, M.B., Senavirathna, M.D.H.J.
Proceedings 38th IAHR World Congress, 2019年09月, ［国際会議］
The electric potential of Nicotiana Benthamiana affected by microwave exposure.
Senavirathna, M.D.H.J., Asaeda, T.
http://en.ifr-pan.krakow.pl/stress-conference, 2018年09月, ［国際会議］
HYDROGEN PEROXIDE AS AN INDICATOR OF ENVIRONMENTAL STRESS INTENSITY OF AQUATIC MACROPHYTES
Asaeda, T., Senavirathna, M.D.H.J.
http://ise2018.com/, 2018年08月, ［国際会議］
EGERIA DENSA DENSITY VS LIGHT INTENSITY: PHOTOSYNTHETIC PIGMENT RESPONSE
Ranawakage, V.P., Senavirathna, M.D.H.J., Asaeda, T.
http://ise2018.com/, 2018年08月, ［国際会議］
EFFECT OF LIGHT INTENSITY ON MYRIOPHYLLUM SPICATUM
Senavirathna, M.D.H.J., Ranawakage, V.P., Asaeda, T.
http://ise2018.com/, 2018年08月, ［国際会議］
EFFECTS OF DIFFERENT LIGHT INTENSITY ON THE GROWTH AND STRESS RESPONSES OF PAEUDANABAENA GALEATA (CYANOBACTERIA)
Muhetare, G., Asaeda, T., Senavirathna, M.D.H.J., Lakmali, H.D.A.
http://ise2018.com/, 2018年08月, ［国際会議］
PALAEOHYDRAULIC PARAMETERS FOR INITIATION SEDIMENT TRANSPORT IN KALIGANDAKI RIVER, NEPAL
Baniya, M.B., Asaeda, T., Senavirathna, M.D.H.J.
http://ise2018.com/, 2018年08月, ［国際会議］
Effects of short duration exposure of different salinity levels on Ceratophyllum demersum
Senavirathna, MDHJ
1911年09月, ［国際会議］
