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KAWAMURA Ryuzo
| Material Science Division | Associate Professor |
| Chemistry |
Researcher information
■ Research Keyword■ Field Of Study
■ Research group
■ Career
- Apr. 2024 - Present
- Apr. 2014 - Mar. 2023, Saitama University, Graduate School of Science and Engineering, Assistant Professor
- Apr. 2012 - Mar. 2014, AIST, Biomedical Research Institute, Cell mechanics research group, Postdoc
- Jan. 2009 - Mar. 2012, RIKEN, ASI MSLS, Postdoc
- Oct. 2008 - Dec. 2008, RIKEN, ASI, MSLS, Research Associate
- Apr. 2005 - Sep. 2008, Hokkaido University, Graduate School of Science
- Apr. 2002 - Mar. 2005, Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology
- Apr. 1998 - Mar. 2002, Tokyo Institute of Technology
■ Award
Performance information
■ Paper- Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells.
Takahisa Matsuzaki; Mai Fujii; Hayata Noro; Shodai Togo; Mami Watanabe; Masami Suganuma; Shivani Sharma; Naritaka Kobayashi; Ryuzo Kawamura; Seiichiro Nakabayashi; Hiroshi Y Yoshikawa
Proceedings of the National Academy of Sciences of the United States of America, Volume：121, Number：50, First page：e2412914121, Dec. 2024, ［International magazine］
We developed an advanced optical microscope for the simultaneous visualization of membrane fluidity and morphology to define cell adhesion signatures. This microscope combines ratiometric spectral imaging of membrane fluidity and interferometric imaging of membrane morphology. As a preliminary demonstration, we simultaneously visualized the interface between a giant unilamellar vesicle (GUV) and a glass substrate at different temperatures. We identified more fluid regions of the membrane and membrane adhesion sites (conversely, low-fluidic, ordered membrane domains correlate with nonadhered regions). This microscopic system was applied to human breast cancer cell lines with different malignancies; then, we identified adhesion signature of cancer cells: 1) low-fluidic, ordered membrane domains at the cell periphery and 2) large fluidic deviation at the nonadhered region. Inhibition of the cholesterol synthesis pathway suppresses the ordered membrane domains at the cancer cell periphery; thus, high level of cholesterol supports the appearance. Furthermore, an inhibitor of the unsaturated lipid synthesis pathway suppressed the large fluidic deviation at the nonadhered region; variation of unsaturated lipids contributes to heterogeneity of the cancer membrane. Therefore, our advanced optical microscopy enables us to couple membrane physical properties with cell adhesion, leading to definition of adhesion signatures of broad cell types, not just for cancer cells, that regulate life phenomena.
English, Scientific journal
DOI：https://doi.org/10.1073/pnas.2412914121
DOI ID：10.1073/pnas.2412914121, PubMed ID：39636859 - In-Situ Observation of DL-Alanine Crystallization from a Laser-Trapped Dense Liquid Droplet as a Heterogeneous Nucleation Site
Hiromasa Niinomi; Hiroshi Y Yoshikawa; Ryuzo Kawamura; Tomoya Yamazaki; Tomoya Oshikiri; Masaru Nakagawa
Chemistry Letters, May 2024, [Reviewed]
Abstract
Nucleation from an aqueous solution is an important step in crystallization which controls the physicochemical properties of crystalline materials. Although dense liquid droplets are considered as a precursor of a crystal in the two-step nucleation model, their actual role is unclear. Our in-situ microscopic observations of the crystallization of DL-alanine from a dense liquid droplet trapped by laser tweezers show that the liquid droplets play the role of a substrate facilitating heterogeneous nucleation rather than a precursor of a crystal.
Oxford University Press (OUP), Scientific journal
DOI：https://doi.org/10.1093/chemle/upae100
DOI ID：10.1093/chemle/upae100, ISSN：0366-7022, eISSN：1348-0715 - Label-free 3D tracking of biological micro-objects using RGB pinholes
Doyeop Kim; Takahisa Matsuzaki; Hiroshi Y. Yoshikawa; Ryuzo Kawamura
Japanese Journal of Applied Physics (Special Issues), Apr. 2024, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.35848/1347-4065/ad3969
DOI ID：10.35848/1347-4065/ad3969 - Mie-Resonant Nanophotonic-Enhancement of Asymmetry in Sodium Chlorate Chiral Crystallization.
Hiromasa Niinomi; Kazuhiro Gotoh; Naoki Takano; Miho Tagawa; Iori Morita; Akiko Onuma; Hiroshi Y Yoshikawa; Ryuzo Kawamura; Tomoya Oshikiri; Masaru Nakagawa
The journal of physical chemistry letters, First page：1564, Last page：1571, Feb. 2024, ［International magazine］
Studies on chiral spectroscopy have recently demonstrated strong enhancement of chiral light-matter interaction in the chiral near-field of Mie resonance in high-refractive-index dielectric nanostructures by studies on chiral spectroscopy. This situation has motivated researchers to demonstrate effective chiral photosynthesis under a chiral near-field beyond circularly polarized light (CPL) as a chiral source. However, the effectivity of the chiral near-field of Mie resonance for chiral photosynthesis has not been clearly demonstrated. One major challenge is the experimental difficulty in evaluating enantiomeric excess of a trace amount of chiral products synthesized in the near-field. Here, by adopting sodium chlorate chiral crystallization as a phenomenon that includes both synthesis and the amplification of chiral products, we show that crystallization on a Mie-resonant silicon metasurface excited by CPL yields a statistically significant large crystal enantiomeric excess of ∼18%, which cannot be achieved merely by CPL. This result provides implications for efficient chiral photosynthesis in a chiral near-field.
English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpclett.3c03303
DOI ID：10.1021/acs.jpclett.3c03303, PubMed ID：38316420 - Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens.
Takahisa Matsuzaki; Ryuzo Kawamura; Akihisa Yamamoto; Hozumi Takahashi; Mai Fujii; Shodai Togo; Yosuke Yoneyama; Fumihiko Hakuno; Shin-Ichiro Takahashi; Masami Suganuma; Seiichiro Nakabayashi; Shivani Sharma; James K. Gimzewski; Hiroshi Y. Yoshikawa
The Journal of Physical Chemistry Letters, Volume：jz-2023-027675 (acs.jpclett.3c02767), Jan. 2024, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpclett.3c02767
DOI ID：10.1021/acs.jpclett.3c02767 - Spatiotemporal Control of Polymorphic Phase Transition of Glycine Crystals by Three-Dimensional Femtosecond Laser Ablation Processing
Hozumi Takahashi; Yudai Yoshimura; Ryota Murai; Ryuzo Kawamura; Mihoko Maruyama; Masashi Yoshimura; Yusuke Mori; Hiroshi Y. Yoshikawa
The Journal of Physical Chemistry Letters, Volume：15, Number：1, First page：180, Last page：186, Dec. 2023
American Chemical Society (ACS), Scientific journal
DOI：https://doi.org/10.1021/acs.jpclett.3c02769
DOI ID：10.1021/acs.jpclett.3c02769, ISSN：1948-7185, eISSN：1948-7185 - Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells
Takahisa Matsuzaki; Yuma Kawano; Momoka Horikiri; Yuko Shimokawa; Takashi Yamazaki; Nao Okuma; Hiroyuki Koike; Masaki Kimura; Ryuzo Kawamura; Yosuke Yoneyama; Yasuro Furuichi; Fumihiko Hakuno; Shin-Ichiro Takahashi; Seiichiro Nakabayashi; Satoshi Okamoto; Hiromitsu Nakauchi; Hideki Taniguchi; Takanori Takebe; Hiroshi Y. Yoshikawa
STAR Protocols, Volume：4, Number：3, First page：102471, Last page：102471, Sep. 2023
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.xpro.2023.102471
DOI ID：10.1016/j.xpro.2023.102471, ISSN：2666-1667 - Surface-limited reactions for spatial control of kinesin–microtubule motility assays using indirect irradiation of an electron beam
Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biomicrofluidics, Volume：16, Number：6, First page：064105, Last page：064105, Dec. 2022
Gliding of microtubules (MTs) on kinesins has been applied to lab-on-a-chip devices, which enable autonomous transportation and detection of biomolecules in the field of bioengineering. For rapid fabrication and evaluation of the kinesin–MT based devices, optical control techniques have been developed for control of kinesin activity and density; however, use of caged molecules lacks spatial controllability for long-term experiments, and direct irradiations of UV light onto kinesin-coated surfaces are inherently damaging to MTs due to their depth limit since the heights of the kinesin–MT systems are at the tens of a nanometer scale. Considering surface electric fields in electrolytic solutions are shielded at the nanometer scale due to Debye shielding, in this study, we show that fine spatial control of kinesin density and activity is enabled using surface-limited electrochemical reactions induced by indirect irradiations of an electron beam (EB). An EB is indirectly irradiated onto the kinesins through a 100-nm-thick silicon nitride membrane, and the electrons scattered in the membrane can cause localized electrochemical effects to the kinesins. We show that these localized electrochemical effects cause both ablation of kinesins and motility control of kinesin activity by changing the EB acceleration voltage. In particular, the latter is achieved without complete ablation of MTs, though the MTs are indirectly irradiated by the EB. As a demonstration of on-demand control of gliding MTs, we show the accumulation of the MTs on a target area by scanning the EB. The proposed accumulation technique will lead to rapid prototyping of microdevices based on MT–kinesin motility assay systems.
AIP Publishing, Scientific journal
DOI：https://doi.org/10.1063/5.0124921
DOI ID：10.1063/5.0124921, eISSN：1932-1058 - Mechanical guidance of self-condensation patterns of differentiating progeny
Takahisa Matsuzaki; Yuko Shimokawa; Hiroyuki Koike; Masaki Kimura; Yuma Kawano; Nao Okuma; Ryuzo Kawamura; Yosuke Yoneyama; Yasuro Furuichi; Fumihiko Hakuno; Shin-Ichiro Takahashi; Seiichiro Nakabayashi; Satoshi Okamoto; Hiromitsu Nakauchi; Hideki Taniguchi; Takanori Takebe; Hiroshi Y. Yoshikawa
iScience, First page：105109, Last page：105109, Sep. 2022
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.isci.2022.105109
DOI ID：10.1016/j.isci.2022.105109, ISSN：2589-0042 - Intelligent gels – artificial soft tissue for the next era
Ken‐Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Polymer International, Volume：71, Number：6, First page：616, Last page：629, Jun. 2022
Wiley, Scientific journal
DOI：https://doi.org/10.1002/pi.6305
DOI ID：10.1002/pi.6305, ISSN：0959-8103, eISSN：1097-0126 - Chiral Optical Force Generated by a Superchiral Near-Field of a Plasmonic Triangle Trimer as Origin of Giant Bias in Chiral Nucleation: A Simulation Study
Hiromasa Niinomi; Teruki Sugiyama; An-Chieh Cheng; Miho Tagawa; Toru Ujihara; Hiroshi Y. Yoshikawa; Ryuzo Kawamura; Jun Nozawa; Junpei T. Okada; Satoshi Uda
The Journal of Physical Chemistry C, Volume：125, Number：11, First page：6209, Last page：6221, Mar. 2021, [Reviewed]
We previously reported that giant crystal enantiomeric excess (CEE) can be obtained when sodium chlorate (NaClO3) chiral crystallization from a solution is induced by the excitation of localized surface plasmon resonance (LSPR) of a Au triangle trimer nanostructure by a circularly polarized laser. However, the role of the LSPR excitation in the giant CEE remains unclear. In this work, we showed, by finite-difference time-domain analysis of plasmonic near-field, that the magnitude of a chiral optical gradient force originating from the strong superchiral near-field at the Au trimer nanogap on a virtual NaClO3 chiral crystalline cluster is comparable to that of the electric-field gradient force in previous laser-trapping-induced crystallization from unsaturated solution. We revealed that the giant CEE resulted from a difference in the frequency of attachment of chiral crystalline clusters to crystal nuclei or in the local concentration due to chirally biased diffusion rather than enantioselective optical trapping.
American Chemical Society (ACS), English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpcc.0c11109
DOI ID：10.1021/acs.jpcc.0c11109, ISSN：1932-7447, eISSN：1932-7455, Web of Science ID：WOS:000635441700026 - Stiffness distribution analysis in indentation depth direction reveals clear mechanical features of cells and organoids by using AFM
Naritaka Kobayashi; Shodai Togo; Takahisa Matsuzaki; Kaede Hashiseko; Ryuzo Kawamura; Masami Suganuma; Seiichiro Nakabayashi; Yosuke Yoneyama; Rie Ouchi; Takanori Takebe; Hiroshi Y Yoshikawa
Applied Physics Express, Volume：13, Number：9, First page：097001, Last page：097001, Sep. 2020, [Reviewed]
Delineating sub-cellular mechanical properties influenced by various cellular components is a challenge in biology in health and disease. We proposed a method to statistically analyze stiffness distribution in indentation depth direction from multiple force curves measured by AFM. The stiffness distributions revealed clear depth-dependent mechanical features of cancer cells and human liver organoids treated with cell/organoid stiffening reagents, which could not be distinguished by conventional analysis that yields averaged stiffness. This work demonstrates that the analytical method is promising for evaluating heterogenous mechanical properties not only of single cell but also of multicellular tissue, and understanding of underlying stiffening mechanisms.
IOP Publishing, English, Scientific journal
DOI：https://doi.org/10.35848/1882-0786/abaeb5
DOI ID：10.35848/1882-0786/abaeb5, ISSN：1882-0778, eISSN：1882-0786, Web of Science ID：WOS:000565743700001 - Plasmonic Manipulation of Sodium Chlorate Chiral Crystallization: Directed Chirality Transfer via Contact-Induced Polymorphic Transformation and Formation of Liquid Precursor
Hiromasa Niinomi; Teruki Sugiyama; Miho Tagawa; Toru Ujihara; Takashige Omatsu; Katsuhiko Miyamoto; Hiroshi Y. Yoshikawa; Ryuzo Kawamura; Jun Nozawa; Junpei T. Okada; Satoshi Uda
Crystal Growth & Design, Volume：20, Number：8, First page：5493, Last page：5507, Aug. 2020, [Reviewed]
The control of crystallization not only impacts the production of functional crystalline materials or pharmaceuticals but also provides profit to investigate the fundamental mechanism of crystallization. Recently, we have revealed that plasmonic optical tweezers (POT) can "manipulate" the crystallization of a pharmaceutical compound from its aqueous solution by optically trapping molecular clusters, offering a novel strategy to control crystallization. Here we report a variety of unique crystallization phenomena by applying POT to sodium chlorate (NaClO3) chiral crystallization from an aqueous microdroplet loaded on a plasmonic substrate. Plasmon excitation significantly promotes crystallization intermediated by an achiral metastable crystal as a precursor. Also, the direction of the creeping of the resulting chiral crystal can be controlled. By utilizing this phenomenon, we achieved the directed chirality transfer from the chiral crystal to the achiral crystal via a forced contact-induced polymorphic transformation by intentionally making creeping chiral crystal contact with an achiral crystal. Moreover, we captured, by in situ optical microscopic observation, a liquid precursor that intermediates the NaClO3 achiral crystallization for the first time. Our results highlight the plasmonic manipulation of crystallization opening a new door not only to control crystallization but also to investigate the unprecedented fundamental process of crystallization.
American Chemical Society (ACS), English, Scientific journal
DOI：https://doi.org/10.1021/acs.cgd.0c00693
DOI ID：10.1021/acs.cgd.0c00693, ISSN：1528-7483, eISSN：1528-7505, Web of Science ID：WOS:000558791100067 - Simultaneous atomic-resolution flexural and torsional imaging in liquid by frequency modulation atomic force microscopy
Megumi Umemoto; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi; Naritaka Kobayashi
JAPANESE JOURNAL OF APPLIED PHYSICS, Volume：59, Jun. 2020, [Reviewed]
Liquid-environment frequency modulation atomic force microscopy (FM-AFM) is a powerful nanotool that can investigate phenomena occurring at solid-liquid interfaces in atomic-scale. To extend its function, we have developed FM-AFM system for simultaneous detection of vertical and lateral tip-sample interaction forces in liquid. Owing to tip-sample distance regulation by using a vertical force as a z-feedback signal, we succeeded in detecting a lateral force stably without full contact of a tip to a sample surface. This enabled simultaneous atomic-resolution flexural and torsional imaging in liquid. We demonstrated that the torsional images, which show different contrast pattern with that of the conventional topographic (flexural) images, emphasize specific structures of the topmost surface such as protruding atoms. (C) 2020 The Japan Society of Applied Physics
IOP PUBLISHING LTD, English, Scientific journal
DOI：https://doi.org/10.35848/1347-4065/ab7479
DOI ID：10.35848/1347-4065/ab7479, ISSN：0021-4922, eISSN：1347-4065, Web of Science ID：WOS:000519637800011 - Quantitative evaluation of the impact of artificial cell adhesion via DNA hybridization on E-cadherin-mediated cell adhesion.
Shodai Togo; Ken Sato; Ryuzo Kawamura; Naritaka Kobayashi; Makoto Noiri; Seiichiro Nakabayashi; Yuji Teramura; Hiroshi Y Yoshikawa
APL bioengineering, Volume：4, Number：1, First page：016103, Last page：016103, Mar. 2020, [Reviewed], ［International magazine］
Programmable cell adhesion with DNA hybridization is a promising approach for fabricating various tissue architectures without sophisticated instrumentation. However, little is known about how this artificial interaction influences the binding of cell adhesion proteins, E-cadherin. In this work, we designed a planar and fluid lipid membrane displaying E-cadherin and/or single-strand DNA with well-defined densities. Visualization of cells on membranes by fluorescence and interference microscopy revealed cell adhesion to be a two-step process: artificial adhesion by DNA hybridization within a few minutes followed by biological adhesion via cadherin-cadherin binding within hours. Furthermore, we discovered that DNA hybridization can substantially facilitate E-cadherin-mediated cell adhesion. The promotive effect is probably due to the enforced binding between E-cadherin molecules in geometrical confinement between two membranes. Our in vitro model of cell adhesion can potentially be used to design functional synthetic molecules that can regulate cell adhesion via cell adhesion proteins for tissue engineering.
English
DOI：https://doi.org/10.1063/1.5123749
DOI ID：10.1063/1.5123749, PubMed ID：32002498, PubMed Central ID：PMC6984976 - Selective electrical switching of molecular motors by dynamic virtual cathode
Kenta Hatazawa; Ryuzo Kawamura; Takayuki Hoshino
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, First page：360, Last page：361, 2020
Environmental and biomedical monitoring bio-applications need the multi-step processing of the chip designs for patterning the biomolecules. This multi-step processing takes a lot of costs to change the biochip design and reconstruct them therefore optimization of the chip function is difficult. So, for reducing processing steps, we propose the on-demand and electrically switched steering of the gliding non-tagged microtubules by the virtual cathode that can generate the electrochemical reactions freely in two dimensional. This method is simpler processing of prototyping than the conventional methods and could be lead to develop the biomedical bio-applications.
International conference proceedings
Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85098277116&origin=inward
Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85098277116&origin=inward
SCOPUS ID：85098277116 - Pause of the target gliding microtuble on the virtual cathode
Kenta Hatazawa; Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biochemical and Biophysical Research Communications, Volume：514, Number：3, First page：821, Last page：825, Jun. 2019, [Reviewed]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.bbrc.2019.04.200
DOI ID：10.1016/j.bbrc.2019.04.200, ISSN：0006-291X - Start/Stop Motion Control of a Targeted Microtubule Using Virtual Cathode Display
Kenta Hatazawa; Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biochem. Biophys. Res. Comm, Volume：514, First page：821, Last page：825, 2019, [Reviewed] - Reversible surface functionalization of motor proteins for sustainable motility
Keisuke Meguriya; Shiori Kikuchi; Naritaka Kobayashi; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi; Ryuzo Kawamura
Jpn. J. Appl. Phys., Volume：58, First page：SDDI01, 2019, [Reviewed]
English, Scientific journal - Light-Powered Nanoconverters Cytotoxic to Breast Cancer Cells
Alexandre R. Loukanov; Alexei G. Basnakian; Ryuzo Kawamura; Hibiki Udono; Chavdar K. Filipov; Alena V. Savenka; Todd Fite; Seiichiro Nakabayashi
Journal of Physical Chemistry C, Volume：122, Number：14, First page：7916, Last page：7924, Apr. 2018, [Reviewed]
We designed and fabricated a light-powered nanoconverter for cytotoxicity toward human breast cancer cells. The nanoconverter was made from highly-fluorescent N-doped carbon nanodots (C-dots), which were covalently conjugated to semiconductive hematite quantum dots (Q-dots). The function of the nanoconverter was to transform the absorbed near-infrared (NIR) irradiation into reactive oxygen species (ROS) which would induce cell death. The principle of operation was based on the photosensitizing properties of C-dots, which have a two-photon absorption cross section. They absorbed NIR irradiation with wavelength in the range of 700-800 nm. The adsorbed energy was upconverted to photoluminescence that is emitted as higher-energy visible light with a maximum wavelength of ∼470 nm and transferred to the Q-dot moiety. The process was accompanied by ejection of electrons from the conduction band of Q-dots and by this mechanism, the nanoconverter produced aqueous superoxide anions, which oxidized organics and generated additional ROS. Our nanoconverter exposed in vitro to cultured human breast cancer HCC1954 cells induced light-dependent cell death as measured using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The cell death was minimal when the cells were exposed with C-dots alone or if the nanoconverter was exposed with the cells in dark.
American Chemical Society, English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpcc.7b11779
Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045390475&origin=inward
Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85045390475&origin=inward
DOI ID：10.1021/acs.jpcc.7b11779, ISSN：1932-7455, eISSN：1932-7455, SCOPUS ID：85045390475 - A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers
Ryuzo Kawamura; Minami Miyazaki; Keita Shimizu; Yuta Matsumoto; Yaron R. Silberberg; Ramachandra Rao Sathuluri; Masumi Iijima; Shun'ichi Ruroda; Futoshi Iwata; Takeshi Kobayashi; Chikashi Nakamura
NANO LETTERS, Volume：17, Number：11, First page：7117, Last page：7124, Nov. 2017, [Reviewed]
Focusing on intracellular targets, we propose a new cell separation technique based on a nano needle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nano needles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nano needles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/acs.nanolett.7b03918
DOI ID：10.1021/acs.nanolett.7b03918, ISSN：1530-6984, eISSN：1530-6992, Web of Science ID：WOS:000415029000091 - Monitoring and extraction of uranium in polluted acid mine drainage by super-paramagnetic nanoparticles coated with carbon nanodots
Alexandre Loukanov; Hibiki Udono; Ryo Takakura; Polina Mladenova; Naritaka Kobayashi; Ryuzo Kawamura; Seiichiro Nakabayashi
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, Volume：314, Number：2, First page：1149, Last page：1159, Nov. 2017, [Reviewed]
In this contribution, we designed environmentally friendly magnetic nanoparticles for simultaneously monitoring and efficiently extracting uranium (VI) from polluted acid mine drainage (> 99% of the total uranium amount). The nanoparticles consist of super-paramagnetic core made of Fe3O4, which is chemically coated with highly-fluorescent shell made of carbon nanodots. The small nanoparticle diameter enables good water dispersibility, high mobility in suspension and powerful sorbent activity to U(VI) in the presence of sulfates, bicarbonates, and pH 4.3-4.8. The measured detection limit of uranium (VI) in model solutions of acid mine drainage was found to be around 0.8 ppm.
SPRINGER, English, Scientific journal
DOI：https://doi.org/10.1007/s10967-017-5471-z
DOI ID：10.1007/s10967-017-5471-z, ISSN：0236-5731, eISSN：1588-2780, Web of Science ID：WOS:000413983300070 - Structural change of nonionic surfactant self-assembling at electrochemically controlled HOPG/electrolyte interface
Naritaka Kobayashi; Hirokazu Saitoh; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, Volume：799, First page：444, Last page：450, Aug. 2017, [Reviewed]
In this study, we have observed structural change of self-assembling nonionic pentaethylene glycol monododecyl ether (C12E5) surfactant formed on a potential-controlled highly oriented pyrolytic graphite (HOPG) surface by electrochemical frequency modulation atomic force microscopy (EC-FM-AFM). At a negative and a positive potential side with respect to the point of zero charge (pzc), a periodic hemicylindrical structure is formed on the whole surface, while a disordered single-layered thin film is formed at a potential around the pzc. This demonstrates that the electrochemical tuning of the electrode potential enables precise control of the nonionic self assembly structure. We also found that the self-assembly structure is expanded by Na+ ion contained in C12E5 solution. This can be reasonably explained by selective cation binding of a C12E5 molecule like a crown ether. In addition, we estimated a response time of phase transition between the two phases by electrochemical impedance measurement.
ELSEVIER SCIENCE SA, English, Scientific journal
DOI：https://doi.org/10.1016/j.jelechem.2017.06.046
DOI ID：10.1016/j.jelechem.2017.06.046, ISSN：1572-6657, eISSN：1873-2569, Web of Science ID：WOS:000411847300059 - Kinesin-Driven Active Substrate Giving Stochastic Mechanical Stimuli to Cells for Characterization
Ryuzo Kawamura; Daiki Uehara; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
ACS BIOMATERIALS SCIENCE & ENGINEERING, Volume：2, Number：12, First page：2333, Last page：2338, Dec. 2016, [Reviewed]
We present a new platform to give stochastic mechanical stimuli to cells for their characterization. There nano- and micrometer scaled fluctuations are generated by an engineered motor protein system of kinesin-microtubules (MTs) on a solid surface. Cells have abilities to deform in many ways during homeostatic metabolism, tissue forming processes, cancer developments, and so on. Namely, cells in biological tissues are exposed to noise-like stochastic movements at nano- and micrometer-scales, which mainly come from the mechanical environment surrounding the cells. Although cells seem to have the potential to respond to such types of mechanical stimuli, the influences on cellular behaviors are poorly understood. As a first attempt to verify an effect of noise-like mechanical stimuli in vitro, we prepared a system to give stochastic mechanical stimuli to cells using a technique of in vitro motility assay for a kinesin-MT system. An active substrate was obtained by integrating movements of MTs on a kinesin-coated glass surface via cross-linkage, and stochastic mechanical stimuli at the cell-scale were successfully applied to the seeded cells. There, traveling distances of the cells over one cell length were observed until they started to adhere. When metastatic melanoma cells were exposed to the stochastic mechanical stimuli, unusually long protrusions or extensions of cell bodies were observed. Cellular aggregations were also promoted through the movements on this active substrate which could disturb the landing and enhance the collisions of the cells. This approach giving mechanical stimuli to cells in a stochastic manner at nano- and micrometer-scales might allow us to uncover unknown behaviors of cells, which might contribute to research fields requiring our understanding on the mechanical nature of cells, such as cancer diagnosis and regenerative medicine.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/acsbiomaterials.6b00538
DOI ID：10.1021/acsbiomaterials.6b00538, ISSN：2373-9878, Web of Science ID：WOS:000389789200023 - High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells
R. Kawamura; K. Shimizu; Y. Matsumoto; A. Yamagishi; Y. R. Silberberg; M. Iijima; S. Kuroda; K. Fukazawa; K. Ishihara; C. Nakamura
JOURNAL OF NANOBIOTECHNOLOGY, Volume：14, First page：74, Nov. 2016, [Reviewed]
Background: The field of structural dynamics of cytoskeletons in living cells is gathering wide interest, since better understanding of cytoskeleton intracellular organization will provide us with not only insights into basic cell biology but may also enable development of new strategies in regenerative medicine and cancer therapy, fields in which cytoskeleton-dependent dynamics play a pivotal role. The nanoneedle technology is a powerful tool allowing for intracellular investigations, as it can be directly inserted into live cells by penetrating through the plasma membrane causing minimal damage to cells, under the precise manipulation using atomic force microscope. Modifications of the nanoneedles using antibodies have allowed for accurate mechanical detection of various cytoskeletal components, including actin, microtubules and intermediate filaments. However, successful penetration of the nanoneedle through the plasma membrane has been shown to vary greatly between different cell types and conditions. In an effort to overcome this problem and improve the success rate of nanoneedle insertion into the live cells, we have focused here on the fluidity of the membrane lipid bilayer, which may hinder nanoneedle penetration into the cytosolic environment.
Results: We aimed to reduce apparent fluidity of the membrane by either increasing the approach velocity or reducing experimental temperatures. Although changes in approach velocity did not have much effect, lowering the temperature was found to greatly improve the detection of unbinding forces, suggesting that alteration in the plasma membrane fluidity led to increase in nanoneedle penetration.
Conclusions: Operation at a lower temperature of 4 degrees C greatly improved the success rate of nanoneedle insertion to live cells at an optimized approach velocity, while it did not affect the binding of antibodies immobilized on the nanoneedle to vimentins for mechanical detection. As these experimental parameters can be applied to various cell types, these results may improve the versatility of the nanoneedle technology to other cell lines and platforms.
BIOMED CENTRAL LTD, English, Scientific journal
DOI：https://doi.org/10.1186/s12951-016-0226-5
DOI ID：10.1186/s12951-016-0226-5, ISSN：1477-3155, Web of Science ID：WOS:000387731300001 - Intelligent gels: Soft and wet nanomachine of the next era
Ken-Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Electrically Active Materials for Medical Devices, First page：253, Last page：268, Sep. 2016, [Reviewed]
We have developed water-swollen hydrogels with excellent mechanical performances and extremely low frictional properties. These gels exhibited motility under electrical stimulus capable of functioning as the artificial muscles. Novel functional gels including electro-conductive functions and tunable photonic crystal have also developed. Adenosine triphosphate (ATP)-driven biomachines made of protein gels will also be introduced.
Imperial College Press, English, In book
DOI：https://doi.org/10.1142/9781783269877_0018
DOI ID：10.1142/9781783269877_0018, SCOPUS ID：85019842820 - Investigation on nanoscale processes on the BaF2(111) surface in various solutions by frequency modulation atomic force microscopy
Naritaka Kobayashi; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi
JOURNAL OF APPLIED PHYSICS, Volume：119, Number：21, First page：214308, Jun. 2016, [Reviewed]
In this study, we have directly observed nanoscale processes that occur on BaF2(111) surfaces in various solutions using liquid-environment frequency modulation atomic force microscopy (FM-AFM) with a true atomic resolution. In addition, to investigate atomic-scale mechanisms of crystal growth process of BaF2, we determined a suitable solution for atomic-resolution FM-AFM imaging of the BaF2(111) surface. For undersaturated solutions, the surface is roughened by barium hydroxo complexes in the case of high pH, whereas by dissolution and proton or water molecule adsorption throughout the surface in the case of low pH. On the other hand, for supersaturated solutions, the surface shows two-dimensional nucleation and growth (sigma = 0.1) and three-dimensional crystal growth with tetrahedral structures (sigma = 1), where sigma is the degree of supersaturation. The atomic-resolution imaging of the BaF2(111) surface has been demonstrated in potassium fluoride (KF) and the supersaturated (sigma = 0.1 and 1) solutions, wherein atomically flat terraces are shown at least for about 30 min. Published by AIP Publishing.
AMER INST PHYSICS, English, Scientific journal
DOI：https://doi.org/10.1063/1.4953233
DOI ID：10.1063/1.4953233, ISSN：0021-8979, eISSN：1089-7550, Web of Science ID：WOS:000378923100024 - Biomimetic Functions of Synthetic Polymer Gels
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano
Hydrogels of Cytoskeletal Proteins, First page：73, Last page：79, Jan. 2016
Springer International Publishing, In book
DOI：https://doi.org/10.1007/978-3-319-27377-8_7
DOI ID：10.1007/978-3-319-27377-8_7 - Design of Polymer Networks Involving a Photoinduced Electronic Transmission Circuit toward Artificial Photosynthesis
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
LANGMUIR, Volume：32, Number：2, First page：626, Last page：631, Jan. 2016, [Reviewed]
Many strategies have been explored to achieve artificial photosynthesis utilizing mediums such as liposomes and supramolecules. Because the photochemical reaction is composed of multiple functional molecules, the surrounding microenvironment is expected to be rationally integrated as observed during photosynthesis in chloroplasts. In this study, photoinduced electronic transmission surrounding the microenvironment of Ru(bpy)3(2+) in a polymer network was investigated using poly(N-isopropylacrylamide-co-Ru(bpy)(3)), poly(acrylamide-co-Ru(bpy)(3)), and Ru(bpy)(3)-conjugated microtubules. Photoinduced energy conversion was evaluated by investigating the effects of (i) Ru(bpy)(3)(2+) immobilization, (ii) polymer type, (iii) thermal energy, and (iv) cross-linking. The microenvironment surrounding copolymerized Ru(bpy)(3)(2+) in poly(N-isopropylacrylamide) suppressed quenching and had a higher radiative process energy than others. This finding is related to the nonradiative process, i.e., photoinduced H-2 generation with significantly higher overall quantum efficiency (13%) than for the bulk solution. We envision that useful molecules will be generated by photoinduced electronic transmission in polymer networks, resulting in the development of a wide range of biomimetic functions with applications for a sustainable society.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/acs.langmuir.5b04326
DOI ID：10.1021/acs.langmuir.5b04326, ISSN：0743-7463, Web of Science ID：WOS:000368563700029 - Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells
Daisuke Matsumoto; Ramachandra Rao Sathuluri; Yoshio Kato; Yaron R. Silberberg; Ryuzo Kawamura; Futoshi Iwata; Takeshi Kobayashi; Chikashi Nakamura
SCIENTIFIC REPORTS, Volume：5, First page：15325, Oct. 2015, [Reviewed]
Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel material and device for the delivery of biomacromolecules into live cells. We attribute the successful results to the unique features of the system, which include high-aspect-ratio, uniform nanoneedles laid across a 2D array, combined with an oscillatory feature, which together allow rapid, forcible and efficient insertion and protein release into thousands of cells simultaneously.
NATURE PUBLISHING GROUP, English, Scientific journal
DOI：https://doi.org/10.1038/srep15325
DOI ID：10.1038/srep15325, ISSN：2045-2322, Web of Science ID：WOS:000362884800001 - Microtubule teardrop patterns
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
SCIENTIFIC REPORTS, Volume：5, First page：9581, Mar. 2015, [Reviewed]
Several strategies for controlling microtubule patterns are developed because of the rigidity determined from the molecular structure and the geometrical structure. In contrast to the patterns in co-operation with motor proteins or associated proteins, microtubules have a huge potential for patterns via their intrinsic flexural rigidity. We discover that a microtubule teardrop pattern emerges via self-assembly under hydrodynamic flow from the parallel bundles without motor proteins. In the growth process, the bundles ultimately bend according to the critical bending curvature. Such protein pattern formation utilizing the intrinsic flexural rigidity will provide broad understandings of self-assembly of rigid rods, not only in biomolecules, but also in supramolecules.
NATURE PUBLISHING GROUP, English, Scientific journal
DOI：https://doi.org/10.1038/srep09581
DOI ID：10.1038/srep09581, ISSN：2045-2322, Web of Science ID：WOS:000351948000001 - Specialized nanoneedles for intracellular analysis
Ryuzo Kawamura; Yaron R. Silberberg; Chikashi Nakamura
Nanobiosensors and Nanobioanalyses, First page：291, Last page：303, Jan. 2015, [Reviewed]
Here, we introduce a novel approach to the detection of intracellular molecules by measuring direct interactions with an ultrathin probe, i.e. nanoneedle, which is mounted on an atomic force microscope (AFM). Standard AFM probes were sharpened, using a focused ion beam (FIB), to form high-aspect-ratio nanoneedles, which were then specifically functionalized and inserted into live cells. The insertion could be precisely detected using the resulted force-distance AFM curves, and no effect on cell viability was observed, even after repeated insertions. In addition, thanks to the high sensitivity of the AFM, distinct intermolecular unbinding events could be analyzed, which provided real-time information on the cytoskeleton state of the live cell. Following specific coatings and functionalization of the nanoneedles, various intracellular molecules could be detected and even inserted into live cells. The results presented here demonstrate the delivery of DNA vectors and the detection of mRNA and cytoskeletal proteins in live cells. Further advances in this technology, such as new developments in molecular functionalization options and improvements in the scale and accuracy of force measurements, will open possible new fields and applications for this diverse and powerful tool.
Springer Japan, English, In book
DOI：https://doi.org/10.1007/978-4-431-55190-4_14
DOI ID：10.1007/978-4-431-55190-4_14, SCOPUS ID：84955671680 - Effect of microtubule polymerization on photoinduced hydrogen generation
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
CHEMICAL COMMUNICATIONS, Volume：51, Number：58, First page：11607, Last page：11610, 2015, [Reviewed]
Herein we report a novel reaction field for photoinduced H-2 generation by using microtubules as a medium. By controlling the tubulin/microtubule hierarchical structure, synergistic effects by which the Ru(bpy)3(2+)-conjugated microtubule network causes suppression of energy loss by collision are clarified.
ROYAL SOC CHEMISTRY, English, Scientific journal
DOI：https://doi.org/10.1039/c5cc02914g
DOI ID：10.1039/c5cc02914g, ISSN：1359-7345, eISSN：1364-548X, Web of Science ID：WOS:000357618200015 - Kosuke Okeyoshi, Ryuzo Kawamura, Ryo Yoshida, Yoshihito Osada
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
Chem. Comm., Volume：51, First page：11607, Last page：11610, 2015, [Reviewed]
English, Scientific journal - Effect of microtubules hierarchy on photoinduced hydrogen generation and application to artificial photosynthesis
Kosuke Okeyoshi; Kawamura Ryuzo; Ryo Yoshida; Yoshihito Osada
Journal of Fluid Mechanics, Volume：1621, Nov. 2014, [Reviewed]
Several strategies have been explored from viewpoint of biomimetics to accomplish artificial photosynthesis by using macromolecules as a medium such as liposomes, supramolecules, and hydrogels.1 Differing from disordered solution systems in which multiple components such as photosensitizer and catalytic nanoparticle are diffusively mixed, the photochemical reactions occur efficiently in medium due to maintenance of the dipersibility of the components and specific molecular arrangement. Here we attempt to clarify the effect of medium hierarchy for photoinduced electronic transmission among multiple components. By conjugating each component on tubulin and integrating them via self-assembly to microtubules, ideal component arrangements with optimum distance for the electronic transmission will be possible.
Cambridge University Press, English, Scientific journal
DOI：https://doi.org/10.1557/opl.2014.174
DOI ID：10.1557/opl.2014.174, ISSN：1469-7645, SCOPUS ID：84908554921 - Measurement of cell adhesion force by vertical forcible detachment using an arrowhead nanoneedle and atomic force microscopy
Seunghwan Ryu; Yui Hashizume; Mari Mishima; Ryuzo Kawamura; Masato Tamura; Hirofumi Matsui; Michiya Matsusaki; Mitsuru Akashi; Chikashi Nakamura
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume：451, Number：1, First page：107, Last page：111, Aug. 2014, [Reviewed]
The properties of substrates and extracellular matrices (ECM) are important factors governing the functions and fates of mammalian adherent cells. For example, substrate stiffness often affects cell differentiation. At focal adhesions, clustered-integrin bindings link cells mechanically to the ECM. In order to quantitate the affinity between cell and substrate, the cell adhesion force must be measured for single cells. In this study, forcible detachment of a single cell in the vertical direction using AFM was carried out, allowing breakage of the integrin-substrate bindings. An AFM tip was fabricated into an arrowhead shape to detach the cell from the substrate. Peak force observed in the recorded force curve during probe retraction was defined as the adhesion force, and was analyzed for various types of cells. Some of the cell types adhered so strongly that they could not be picked up because of plasma membrane breakage by the arrowhead probe. To address this problem, a technique to reinforce the cellular membrane with layer-by-layer nanofilms composed of fibronectin and gelatin helped to improve insertion efficiency and to prevent cell membrane rupture during the detachment process, allowing successful detachment of the cells. This method for detaching cells, involving cellular membrane reinforcement, may be beneficial for evaluating true cell adhesion forces in various cell types. (C) 2014 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, English, Scientific journal
DOI：https://doi.org/10.1016/j.bbrc.2014.07.078
DOI ID：10.1016/j.bbrc.2014.07.078, ISSN：0006-291X, eISSN：1090-2104, Web of Science ID：WOS:000340864200017 - Employing cytoskeletal treadmilling in bio-actuator
Ken-Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Soft Actuators: Materials, Modeling, Applications, and Future Perspectives, Volume：9784431547679, First page：489, Last page：497, Jul. 2014, [Reviewed]
In this chapter, we describe treadmilling bio-actuators. The principle of treadmilling actuator is, filamentous protein complex formation by actin or tubulin accompanying a sequence of nucleotide triphosphate hydrolysis is to alter the critical concentration of polymerization at the two ends of the filament. Recently, we have succeeded in the creation of hydrogels which autonomously oscillate owing to the treadmilling of actin or tubulin. These hydrogels have great potential as bio-actuators because they are easy to make on a centimeter scale.
Springer Japan, English, In book
DOI：https://doi.org/10.1007/978-4-431-54767-9_35
DOI ID：10.1007/978-4-431-54767-9_35, SCOPUS ID：84930712258 - Effect of microtubules hierarchy on photoinduced hydrogen generation and application to artificial photosynthesis
Kosuke Okeyoshi; Kawamura Ryuzo; Ryo Yoshida; Yoshihito Osada
Materials Research Society Symposium Proceedings, Volume：1621, 2014, [Reviewed]
Several strategies have been explored from viewpoint of biomimetics to accomplish artificial photosynthesis by using macromolecules as a medium such as liposomes, supramolecules, and hydrogels.1 Differing from disordered solution systems in which multiple components such as photosensitizer and catalytic nanoparticle are diffusively mixed, the photochemical reactions occur efficiently in medium due to maintenance of the dipersibility of the components and specific molecular arrangement. Here we attempt to clarify the effect of medium hierarchy for photoinduced electronic transmission among multiple components. By conjugating each component on tubulin and integrating them via self-assembly to microtubules, ideal component arrangements with optimum distance for the electronic transmission will be possible. Copyright © Materials Research Society 2014.
Materials Research Society, English, International conference proceedings
DOI：https://doi.org/10.1557/opl.2014.174
DOI ID：10.1557/opl.2014.174, ISSN：0272-9172, SCOPUS ID：84897974976 - Controlling the bias of rotational motion of ring-shaped microtubule assembly
Shoki Wada; Arif Md; Rashedul Kabir; Ryuzo Kawamura; Masaki Ito; Daisuke Inoue; Kazuki Sada; Akira Kakugo
Biomacromolecules, Volume：16, First page：374, Last page：378, 2014, [Reviewed]
English, Scientific journal - Chemically cross-linked microtubule assembly shows enhanced dynamic motions on kinesins
Ryuzo Kawamura; Ken-Ichi Sano; Kuniharu Ijiro; Yoshihito Osada
RSC ADVANCES, Volume：4, Number：62, First page：32953, Last page：32959, 2014, [Reviewed]
Biomolecular motors have cooperative functions that facilitate various important cellular functions such as signaling and deformation. However, the multiple-molecular behavior is poorly understood when compared with the well-studied behavior of single molecules. Kinesin and microtubules (MTs) are a combination of motor and rail proteins that have been reported to show cooperativity in moving patterns. Here, we report that cross-linking of isotropic MTs enhances the movement of the local network temporally, which can elicit the effective conveyance of 5 mu m diameter silica microbeads. The average velocity of the beads reached 143 nm s(-1) for over 15 min, which is 2.7 times higher than the beads on kinesin-driven MTs without cross-links. Although the velocity of the beads fluctuated over the time course, the maximum velocity calculated from 5 s displacement reached 1.2 mu m s(-1), which was ca. 3-fold higher than the intrinsic driving velocity of kinesins, i.e., the sliding velocity of the MTs without cross-links. This technique provides such an enhanced movement by simply cross-linking the isotropic MTs, but without specific control or alignment over network polarity. The enhancement in the local motion of the MTs is discussed in terms of the "mechanochemical" effect coupled between dynamically stored elastic energies in the MT network and the collective motion of MTs driven by an ATP-fueled kinesin motor.
ROYAL SOC CHEMISTRY, English, Scientific journal
DOI：https://doi.org/10.1039/c4ra04491f
DOI ID：10.1039/c4ra04491f, ISSN：2046-2069, Web of Science ID：WOS:000341083400049 - Thermo- and photo-enhanced microtubule formation from Ru(bpy)(3)(2+)-conjugated tubulin
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
JOURNAL OF MATERIALS CHEMISTRY B, Volume：2, Number：1, First page：41, Last page：45, 2014, [Reviewed]
Ru(bpy)(3)(2+)-conjugated tubulin is able to substantially enhance polymerization to form microtubules with increased rate at lower temperatures. Additionally, the polymerization is enhanced by photo-irradiation and the possible mechanism is discussed focusing on the photo-thermal energy conversion.
ROYAL SOC CHEMISTRY, English, Scientific journal
DOI：https://doi.org/10.1039/c3tb21242d
DOI ID：10.1039/c3tb21242d, ISSN：2050-750X, eISSN：2050-7518, Web of Science ID：WOS:000327607100005 - Detection of microtubules in vivo using antibody-immobilized nanoneedles
Yaron R. Silberberg; Ryuzo Kawamura; Seunghwan Ryu; Kyoko Fukazawa; Kazuhiko Ishihara; Chikashi Nakamura
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Volume：117, Number：1, First page：107, Last page：112, Jan. 2014, [Reviewed]
We present here an alternative, force-based measurement method for the detection of intracellular cytoskeletal proteins in the live cell. High aspect ratio nanoneedles of 200 nm in diameter were functionalized with anti-tubulin antibodies and inserted, using an atomic force microscope (AFM), into live NIH3T3 cells, without affecting cell viability. Force curves were recorded during insertion and evacuation of nanoneedles from the cells, and used to analyse intracellular interactions of the nanoneedles with the microtubule cytoskeleton during evacuation from the cell. Disruption of microtubules led to a correlated time-dependent decrease in the measured intracellular binding forces, pointing to the high-sensitivity and high-specificity of this detection method. This analytical technique allows for real-time evaluation of the microtubule network in the live cell, without the need to use potentially harmful molecular markers as do conventional detection methods, and may prove beneficial in the diagnosis and investigation of cytoskeleton-associated diseases. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, English, Scientific journal
DOI：https://doi.org/10.1016/j.jbiosc.2013.06.019
DOI ID：10.1016/j.jbiosc.2013.06.019, ISSN：1389-1723, eISSN：1347-4421, Web of Science ID：WOS:000333071300018 - Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA
Seunghwan Ryu; Ryuzo Kawamura; Ryohei Naka; Yaron R. Silberberg; Noriyuki Nakamura; Chikashi Nakamura
Journal of Bioscience and Bioengineering, Volume：116, Number：3, First page：391, Last page：396, Sep. 2013, [Reviewed]
An atomic force microscope probe can be formed into an ultra-sharp cylindrical shape (a nanoneedle) using micro-fabrication techniques such as focused ion beam etching. This nanoneedle can be effectively inserted through the plasma membrane of a living cell to not only access the cytosol, but also to penetrate through the nuclear membrane. This technique shows great potential as a tool for performing intranuclear measurements and manipulations. Repeated insertions of a nanoneedle into a live cell were previously shown not to affect cell viability. However, the effect of nanoneedle insertion on the nucleus and nuclear components is still unknown. DNA is the most crucial component of the nucleus for proper cell function and may be physically damaged by a nanoneedle. To investigate the integrity of DNA following nanoneedle insertion, the occurrence of DNA double-strand breaks (DSBs) was assessed. The results showed that there was no chromosomal DNA damage due to nanoneedle insertion into the nucleus, as indicated by the expression level of γ-H2AX, a molecular marker of DSBs. © 2013 The Society for Biotechnology, Japan.
English, Scientific journal
DOI：https://doi.org/10.1016/j.jbiosc.2013.03.022
DOI ID：10.1016/j.jbiosc.2013.03.022, ISSN：1389-1723, PubMed ID：23643618, SCOPUS ID：84881101246 - Controlled cell adhesion using a biocompatible anchor for membrane-conjugated bovine serum albumin/bovine serum albumin mixed layer
Ryuzo Kawamura; Mari Mishima; Seunghwan Ryu; Yu Arai; Motomu Okose; Yaron R. Silberberg; Sathuluri Ramachandra Rao; Chikashi Nakamura
Langmuir, Volume：29, Number：21, First page：6429, Last page：6433, May 2013, [Reviewed]
DOI：https://doi.org/10.1021/la4012229
DOI ID：10.1021/la4012229, ISSN：0743-7463, PubMed ID：23639009 - 2P179 Evaluation of cell adhesion force by mechanical detachment using AFM(12. Cell biology,Poster)
Mishima Mari; Kawamura Ryuzo; Okada Tomoko; Nakamura Chikashi
Seibutsu Butsuri, Volume：53, Number：1, First page：S188, 2013
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.53.S188_5
DOI ID：10.2142/biophys.53.S188_5, CiNii Articles ID：110009819668 - Microtuhular teardrop patterning and the growing process
Kosuke Okeyoshi; Kawamura Ryuzo; Yoshihito Osada
Materials Research Society Symposium Proceedings, Volume：1504, First page：22, Last page：28, 2013, [Reviewed]
Here we show that microtubular bundles bend flexibly under a hydrodynamic flow to form teardrop patterns, [n a highly concentrated microtubular solution, patterns of same-sized teardrops form according to the maximum critical curvature, which is determined by the specific rigidity of the microtubules. Our understanding is that these micropatterns grow when microtubular bundles with hydrodynamic flow energy are converted into stable teardrop patterns as a higher structure. This conversion is generated by the combined effect of multiple kinds of energy, including heat and hydrodynamic flow, as well as life systems. These self-generating patterns in a spatio-temporal stream are reminiscent of what the artist Edward Munch called a scream of nature. We also envision that microtubular pattering with hierarchical structure will broaden the potential application of these geometrical structures and guide biomimetic material engineering towards areas such as integrated energy conversion, soft material patterning, and living signal transduction. © 2013 Materials Research Society.
Materials Research Society, English, International conference proceedings
DOI：https://doi.org/10.1557/opl.2013.243
DOI ID：10.1557/opl.2013.243, ISSN：0272-9172, SCOPUS ID：84899871585 - Controlled cell adhesion using a BAM-BSA/BSA mixed layer
Ryuzo Kawamura; Mari Mishima; Seunghwan Ryu; Yu Arai; Motomu Okose; Yaron R. Silberberg; Sathuluri Ramachandra Rao; Chikashi Nakamura
Langmuir, Volume：29, First page：6429, Last page：6433, 2013, [Reviewed]
English, Scientific journal - Nanopattern Fabrication of Gold on Hydrogels and Application to Tunable Photonic Crystal
Naonobu Shimamoto; Yoshito Tanaka; Hideyuki Mitomo; Ryuzo Kawamura; Kuniharu Ijiro; Keiji Sasaki; Yoshihito Osada
ADVANCED MATERIALS, Volume：24, Number：38, First page：5243, Last page：5248, Oct. 2012, [Reviewed]
WILEY-V C H VERLAG GMBH, English, Scientific journal
DOI：https://doi.org/10.1002/adma.201201522
DOI ID：10.1002/adma.201201522, ISSN：0935-9648, eISSN：1521-4095, Web of Science ID：WOS:000309176800013 - Nematic growth of microtubules that changed into giant spiral structure through partial depolymerization and subsequent dynamic ordering
Kiyotaka Shigehara; Hiroki Kudoh; Saori Mori; Yoshiki Tamura; Akira Kakugo; Ryuzo Kawamura; Hidemitsu Furukawa; Jian Ping Gong; Hiroyasu Masunaga; Tomomi Masui; Satoshi Koizumi; Kazuhiro Shikinaka
SOFT MATTER, Volume：8, Number：45, First page：11544, Last page：11551, 2012, [Reviewed]
In a long capillary cell with temperature gradient, tubulin dimers with alpha and beta subunits polymerized according to the preferential polarity, i.e., the anisotropic spiral addition of the dimers to the beta-terminated "plus end" dominated the formation of microtubules. As the result, the helical hollow cylinders generated the oriented nematic liquid crystalline structure with centimeter-length. In the next stage, where microtubules were under the partial polymerization/depolymerization equilibrium due to the concentration fluctuation, the dynamic rearrangement of microtubules such as their shortening (depolymerization) and subsequent tilting of orientation axis caused the structural change from the oriented nematic liquid crystalline structure to some giant spiral structure which was subjected by the ordered dipole and the helical chirality of microtubules.
ROYAL SOC CHEMISTRY, English, Scientific journal
DOI：https://doi.org/10.1039/c2sm26675j
DOI ID：10.1039/c2sm26675j, ISSN：1744-683X, eISSN：1744-6848, Web of Science ID：WOS:000310829800018 - Formation of motile assembly of microtubules driven by kinesins
Ryuzo Kawamura; Akira Kakugo; Kazuhiro Shikinaka; Yoshihito Osada; Jian Ping Gong
SMART MATERIALS AND STRUCTURES, Volume：20, Number：12, First page：124007, Dec. 2011, [Reviewed]
Microtubule (MT) and kinesin are rail and motor proteins that are involved in various moving events of eukaryotic cells in natural systems. In vitro, the sliding motion of microtubules (rail) can be reproduced on a kinesin (motor protein)-coated surface coupled with adenosine triphosphate (ATP) hydrolysis, which is called a `motility assay'. Based on this technique, a method was recently established to form MT assemblies by an active self-assembly (AcSA) process, in which MTs are crosslinked during a sliding motion on a kinesin-coated surface. Streptavidin (ST) was employed as glue to crosslink biotin-labeled MTs. Various shapes, sizes, and motilities were formed with the AcSA MT assemblies, depending on the initial conditions. In this paper, we briefly review our recent work on the formation of MT assemblies on a kinesin-coated surface.
IOP PUBLISHING LTD, English, Scientific journal
DOI：https://doi.org/10.1088/0964-1726/20/12/124007
DOI ID：10.1088/0964-1726/20/12/124007, ISSN：0964-1726, eISSN：1361-665X, Web of Science ID：WOS:000298140200008 - Self-Repairing Filamentous Actin Hydrogel with Hierarchical Structure
Ken-Ichi Sano; Ryuzo Kawamura; Taiki Tominaga; Naoko Oda; Kuniharu Ijiro; Yoshihito Osada
BIOMACROMOLECULES, Volume：12, Number：12, First page：4173, Last page：4177, Dec. 2011, [Reviewed]
A chemically cross-linked filamentous actin (F-actin) gel consisting of globular actin (G-actin) as repeating units was prepared. The F-actin gel was cross-linked by covalent bonds, and the main chain is represented by the self assembly of G-actin with a high-ordered hierarchical structure. The gel. exhibited good mechanical performance with a storage modulus >1 kPa and undergoes reversible sol-gel transitions. in response to changes in the salt concentration (chemical induced sol-gel transition) as well as to shear strain (mechanical-induced sol-gel transition). Therefore, the gel exhibits self repairing ability through dynamic polymerization and depolymerization across the structure hierarchies under repeated shear stress.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/bm2009922
DOI ID：10.1021/bm2009922, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000297782100001 - Thermoresponsive Microtubule Hydrogel with High Hierarchical Structure
Ken-Ichi Sano; Ryuzo Kawamura; Taiki Tominaga; Hiromichi Nakagawa; Naoko Oda; Kuniharu Ijiro; Yoshihito Osada
BIOMACROMOLECULES, Volume：12, Number：5, First page：1409, Last page：1413, May 2011, [Reviewed]
A thermoresponsive 3D microtubule hydrogel (MT gel) was prepared by simultaneous polymerization and chemical cross-linking of tubulins. The main chain of this gel is composed of cross-linked MTs, which consists of a cylindrical assembly of tubulin covalently connected by polyethylene glycol. This gel, which contains 10 mg/mL of tubulin, exhibits a storage modulus G' as high as 1 x 10(3), which is 10 times higher than the loss modulus G '' over a wide range of frequencies. The MT gel exhibits a reversible sol gel transition by temperature changes at 4-37 degrees C via depolymerization and polymerization of the MT network. Notable effects of the presence of the cross-linkage on the process of polymerization and depolymerization of tubulin were experimentally observed, and the role of the cross-linkage was discussed.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/bm101578x
DOI ID：10.1021/bm101578x, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000290246400002 - 3J1458 Motility of chemically cross-linked microtubule network driven by kinesin(Bioengineering2,The 49th Annual Meeting of the Biophysical Society of Japan)
Kawamura Ryuzo; Sano Ken-ichi; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
Seibutsu Butsuri, Volume：51, First page：S143, 2011
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.51.S143_2
DOI ID：10.2142/biophys.51.S143_2, CiNii Articles ID：110008903569 - Dynamic self-organization and polymorphism of microtubule assembly through active interactions with kinesin
Yoshiki Tamura; Ryuzo Kawamura; Kazuhiro Shikinaka; Akira Kakugo; Yoshihito Osada; Jian Ping Gong; Hiroyuki Mayama
SOFT MATTER, Volume：7, Number：12, First page：5654, Last page：5659, 2011, [Reviewed]
In this study, we show that the energy-dissipative active self-assembly of microtubules (MTs) via a kinesin-based motility system produces various MT assemblies such as bundle-, network-, and ring-shaped structures depending on the initial conditions. Structural polymorphism of the MT assembly is depicted through phase diagrams, and morphogenesis of the MT assembly is discussed based on the following factors: binding force between MTs and motility-driving force from kinesins. This study provides new insights into the energy-dissipative dynamic self-organization of biological systems.
ROYAL SOC CHEMISTRY, English, Scientific journal
DOI：https://doi.org/10.1039/c1sm05413a
DOI ID：10.1039/c1sm05413a, ISSN：1744-683X, eISSN：1744-6848, Web of Science ID：WOS:000291354200025 - Microtubule bundle formation driven by ATP: the effect of concentrations of kinesin, streptavidin and microtubules
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong
NANOTECHNOLOGY, Volume：21, Number：14, First page：145603, Apr. 2010, [Reviewed]
Recently, a method was established for the formation of microtubule (MT) assemblies by an active self-organization (AcSO) process, in which MTs were crosslinked during sliding motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate (ATP) hydrolysis. Streptavidin (ST) was the glue used to crosslink biotin-labeled MTs. Although most of the MT assemblies were in the bundle form, they varied in size, shape and motility, depending on the initial conditions used. In this paper, we systematically examined the effects of the concentrations of kinesin, ST and MT on the formation of MT bundles under the initial conditions of the process.
IOP PUBLISHING LTD, English, Scientific journal
DOI：https://doi.org/10.1088/0957-4484/21/14/145603
DOI ID：10.1088/0957-4484/21/14/145603, ISSN：0957-4484, eISSN：1361-6528, Web of Science ID：WOS:000275652200029 - 1P155 1D1505 Autonomous oscillation in mechanical property of actin gel(Muscle,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)
Kawamura Ryuzo; Sano Ken-Ichi; Tominaga Taiki; Ijiro Kuniharu; Osada Yoshihito
Seibutsu Butsuri, Volume：50, Number：2, First page：S46, 2010
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.50.S46_5
DOI ID：10.2142/biophys.50.S46_5, CiNii Articles ID：110008102391 - 1P156 Reversible gel-sol transition of covalently cross-linked actin hydrogel(Muscle,The 48th Annual Meeting of the Biophysical Society of Japan)
Sano Ken-Ichi; Kawamura Ryuzo; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
Seibutsu Butsuri, Volume：50, Number：2, First page：S46, Last page：S47, 2010
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.50.S46_6
DOI ID：10.2142/biophys.50.S46_6, CiNii Articles ID：110008102392 - Selective Formation of a Linear-Shaped Bundle of Microtubules
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong
LANGMUIR, Volume：26, Number：1, First page：533, Last page：537, Jan. 2010, [Reviewed]
By using rigid microtubules (MTs) prepared by polymerization with guanylyl-(alpha, beta)-methylene-diphosphonate GMPCPP, giant straight-shaped MT bundles were selectively obtained through a dynamic self-assembly process. We demonstrate the effect of the rigidity oil the shape and motility of MT bundle composed of GMPCPP-polymerized MTs (GMPCPP-MTs) compared with control MTs that were polymerized with GTP and stabilized with paclitaxel.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/la902197f
DOI ID：10.1021/la902197f, ISSN：0743-7463, Web of Science ID：WOS:000272937500072 - Formation of Well-Oriented Microtubules with Preferential Polarity in a Confined Space under a Temperature Gradient
Akira Kakugo; Yoshiki Tamura; Kazuhiro Shikinaka; Momoko Yoshida; Ryuzo Kawamura; Hidemitsu Furukawa; Yoshihito Osada; Jian Ping Gong
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Volume：131, Number：50, First page：18089, Last page：18095, Dec. 2009, [Reviewed]
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/ja901538n
DOI ID：10.1021/ja901538n, ISSN：0002-7863, Web of Science ID：WOS:000273615400031 - 3P-266 Thermo-reversible hydrogel formed by microtubules(Bioengineering,The 47th Annual Meeting of the Biophysical Society of Japan)
Kawamura Ryuzo; Sano Ken-Ichi; Kunita Istuki; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
Seibutsu Butsuri, Volume：49, First page：S195, 2009
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.49.S195_3
DOI ID：10.2142/biophys.49.S195_3, CiNii Articles ID：110008102120 - (Dissertation) Study on dynamic sefl-assembly of microtubules by kinesin
Kawamura Ryuzo
Hokkaido University, Dec. 2008, [Reviewed]
English, Doctoral thesis - Ring-shaped assembly of microtubules shows preferential counterclockwise motion
Ryuzo Kawamura; Akira Kakugo; Kazuhiro Shikinaka; Yoshihito Osada; Jian Ping Gong
BIOMACROMOLECULES, Volume：9, Number：9, First page：2277, Last page：2282, Sep. 2008, [Reviewed]
In this paper, we reveal that microtubules (MTs), reconstructed from tubulin in vitro in the presence of guanosine-5'-triphosphate (GTP), have a ring or spiral shape on a motor protein-fixed surface, and these MTs show biased motion in the counterclockwise direction. By cross-linking these MTs during the sliding motion, we obtained large ring-shaped MT assemblies, 1 similar to 12.6 mu m in diameter. The ratio of the rings rotating in the counterclockwise direction to those rotating in the clockwise direction was approximately 3/1. Under optimized conditions, the ratio was as high as 14/1. Thus, We Successfully obtained aggregated MTs with a large hierarchic structure that shows a preferential motion, through a dynamic process in vitro.
AMER CHEMICAL SOC, English, Scientific journal
DOI：https://doi.org/10.1021/bm800639w
DOI ID：10.1021/bm800639w, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000259033700001 - Integration of Biomolecular Motors: Toward an ATP Fueled Soft Moving System
Kazuhiro Shikinaka; Ryuzo Kawamura; Akira Kakugo; Hidemitsu Furukawa; Jian Ping Gong; Yoshihito Osada
Polymer Preprints, Japan, Volume：57, First page：106, 2008
English, Scientific journal - 2P161 Dynamic self-assembly of microtubule complex by kinesin : toward ATP-fueled actuator(Molecular motors,Poster Presentations)
Kawamura Ryuzo; Kakugo Akira; Furukawa Hidemitsu; Gong Jian Ping; Osada Yoshihito
Seibutsu Butsuri, Volume：47, First page：S153, 2007
The Biophysical Society of Japan General Incorporated Association, English
DOI：https://doi.org/10.2142/biophys.47.S153_2
DOI ID：10.2142/biophys.47.S153_2, CiNii Articles ID：110006562252 - Transduction of NeuroD2 protein induced neural cell differentiation
Tomohide Noda; Ryuzo Kawamura; Hisakage Funabashi; Masayasu Mie; Eiry Kobatake
JOURNAL OF BIOTECHNOLOGY, Volume：126, Number：2, First page：230, Last page：236, Nov. 2006, [Reviewed]
NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta 100-115) (lack of NLS), ND2(Delta 123-134) (lack of basic) and ND2(Delta 100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein. (c) 2006 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, English, Scientific journal
DOI：https://doi.org/10.1016/j.jbiotec.2006.04.021
DOI ID：10.1016/j.jbiotec.2006.04.021, ISSN：0168-1656, eISSN：1873-4863, Web of Science ID：WOS:000241783300012 - Doxorubicin encapsulation and diffusional release from stable, polymeric, hydrogel nanoparticles
Dimitrios Missirlis; Ryuzo Kawamura; Nicola Tirelli; Jeffrey A. Hubbell
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, Volume：29, Number：2, First page：120, Last page：129, Oct. 2006, [Reviewed]
We have recently described the preparation of stable, polymeric nanoparticles, composed of poly(ethylene glycol) and poloxamer 407 (Pluronic (R) F127), prepared via inverse emulsion photopolymerization. In the present study we report on the performance of this novel colloidal system as a controlled delivery system for small hydrophobic drugs. Successful encapsulation of doxorubicin occurred through hydrophobic interactions, taking advantage of particle nanoarchitecture. Loadings of up to 8.7 wt.% were achieved with a reproducible, fast, solvent evaporation procedure. In vitro drug release, monitored by fluorescence spectrometry and HPLC, revealed a minor burst (approximately 10% at 37 degrees C) and sustained, diffusional release for over 1 week; furthermore, drug encapsulation significantly delayed doxorubicin degradation kinetics. (c) 2006 Published by Elsevier B.V.
ELSEVIER SCIENCE BV, English, Scientific journal
DOI：https://doi.org/10.1016/j.ejps.2006.06.003
DOI ID：10.1016/j.ejps.2006.06.003, ISSN：0928-0987, Web of Science ID：WOS:000241372100004
- ポリマー材料中におけるカーボンドットのメカノクロミズム機能探索
川村隆三; LOUKANOV Alexandre
Volume：31, 2019
ISSN：1344-0497, J-Global ID：201902236196531935 - バーチャル電極ディスプレイの微小管滑走アッセイでの局所性評価
畑澤研太; 宮廻裕樹; 川村隆三; 星野隆行; 星野隆行
Volume：66th, 2019
J-Global ID：201902248990834557 - DNA-PEG脂質を介した細胞接着機構の解明-細胞組織形成の自在制御に向けて-
東郷祥大; 佐藤健; 川村隆三; 小林成貴; 中林誠一郎; 寺村裕治; 吉川洋史
Volume：98th, 2018
J-Global ID：201802233641288888 - DNA hybridizationが細胞接着に与える影響の解明-細胞組織形成の人為的誘導に向けて-
佐藤健; 寺村裕治; 川村隆三; 小林成貴; 中林誠一郎; 吉川洋史
Volume：64th, 2017
J-Global ID：201702276065767041 - DNA-PEG脂質を用いた細胞接着ダイナミクスの解明
佐藤健; 寺村裕治; 川村隆三; 中林誠一郎; 吉川洋史
Volume：96th, 2016
J-Global ID：201602266545753865 - Hydrogels of cytoskeletal proteins: Preparation, structure, and emergent functions
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano
Hydrogels of Cytoskeletal Proteins: Preparation, Structure, and Emergent Functions, First page：1, Last page：79, 01 Jan. 2016
This book describes a new family of bio-polymer gels made from cytoskeletal proteins - actin, microtubule, and tropomyosin. The importance of the gel state with multi-scale hierarchical structure is emphasized to utilize emergent functions in living organisms. Detailed protocol of gel preparation, specified method of structure investigation, and dynamic studies of self-organization, self-healing, synchronized oscillating, and autonomous motility functions are introduced together with biomimetic functions of synthetic hydrogels.
Springer International Publishing, English, Others
DOI：https://doi.org/10.1007/978-3-319-27377-8
DOI ID：10.1007/978-3-319-27377-8, SCOPUS ID：84958879297 - ナノニードルアレイを用いたCreタンパク質の核内輸送
松本大亮; 齋藤恵; 加藤義雄; 川村隆三; 小林健; 岩田太; 中村史
Volume：95th, Number：3, 2015
ISSN：0285-7626, J-Global ID：201502228386765154 - ネスチンを標的とした神経幹細胞の機械的分離技術の開発
宮崎みなみ; 清水桂太; 松本大亮; 川村隆三; 小林健; 飯嶋益巳; 黒田俊一; 岩田太; 中村史; 中村史
Volume：82nd, 2015
J-Global ID：201502285332423448 - 3P-206 Mechanical cell separation of undifferentiated iPS cells targeting vimentin
Shimizu Keita; Kawamura Ryuzo; Kobayashi Takeshi; Iijima Masumi; Kuroda Shun'ichi; Iwata Futoshi; Nakamura Chikashi
Volume：67, First page：322, Last page：322, 2015
Japanese
CiNii Articles ID：110010009621, CiNii Books ID：AN10549378 - 1P-257 Vimentin detection from undifferentiated iPS cells using antibodyfunctionalized nanoneedle
Shimizu Keita; Kawamura Ryuzo; Iijima Masumi; Kuroda Shun'ichi; Fukazawa Kyoko; Ishihara Kazuhiko; Nakamura Chikashi
Volume：66, First page：82, Last page：82, 2014
Japanese
CiNii Articles ID：110009906338, CiNii Books ID：AN10549378 - Treadmill Machine of Cytoskeletal Proteins, Toward Establishment of Bio-actuator
KAWAMURA Ryuzo; SANO Ken-Ichi; OSADA Yoshihito
Volume：48, Number：1, First page：11, Last page：15, 05 Jan. 2013
Japanese
ISSN：0919-2948, CiNii Articles ID：10031146082, CiNii Books ID：AN10402165 - 2P-172 Mechanical cell separation using antibody-immobilized nanoneedle
Miyazaki Minami; Shimizu Keita; Kawamura Ryuzo; Sathuluri Ramachandra Rao; Kobayashi Takeshi; Iijima Masumi; Kuroda Shun'ichi; Fukazawa Kyoko; Ishihara Kazuhiko; Iwata Futoshi; Nakamura Chikashi
Volume：65, First page：147, Last page：147, 2013
Japanese
CiNii Articles ID：110009737838, CiNii Books ID：AN10549378 - 2P-171 Controlled cell adhesion using a BAM-BSA/BSA mixed layer
Mishima Mari; Kawamura Ryuzo; Okose Motomu; Nakamura Chikashi
Volume：65, First page：147, Last page：147, 2013
Japanese
CiNii Articles ID：110009737837, CiNii Books ID：AN10549378 - 2Bp15 Effect of nanoneedle oscillation on the insertion into living cells
Ryu Seung-Hwan; Silberberg Yaron; Kawamura Ryuzo; Nakamura Chikashi
Volume：64, First page：31, Last page：31, 2012
Japanese
CiNii Articles ID：110009609366, CiNii Books ID：AN10549378 - 2Bp14 A method to modify nanoneedle arrays with antibodies homogeneously
Shimooku Marie; Kawamura Ryuzo; Ishihara Kazuhiko; Fukazawa Kyoko; Nakamura Chikashi
Volume：64, First page：31, Last page：31, 2012
Japanese
CiNii Articles ID：110009609365, CiNii Books ID：AN10549378 - ATP駆動型ソフトアクチュエーターの創製-配向微小管の極性評価および形成メカニズムの解明-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; きょう剣萍
Volume：44th, 2010
J-Global ID：201002246797602010 - ATP駆動型アクチュエータの創製-配向微小管集合体の作製とその極性評価-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
Volume：58, Number：2 Disk1, 2009
J-Global ID：200902209211232097 - 配向微小管によるアクティブトランスポーターの創製
吉田桃子; 敷中一洋; 川村隆三; 田村圭輝; 角五彰; 角五彰; 古川英光; きょう剣萍
Volume：43rd, 2009
J-Global ID：200902241524606665 - ATP駆動型アクチュエータの創製-配向微小管集合体の作製とその運動評価-
吉田桃子; 田村圭輝; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
Volume：20th, 2009
J-Global ID：200902252143173012 - ATP駆動型ソフトアクチュエーターの創製-配向微小管集合体の作製とその運動評価-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
Volume：64, Number：1, 2009
J-Global ID：200902253094425287 - 配向微小管によるアクティブトランスポーターの創製
吉田桃子; 田村圭樹; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
Volume：58, Number：1 Disk1, 2009
J-Global ID：200902254038450402 - Integration of biomolecular motors Toward an ATP fueled soft biomachine
Akira Kakugo; Kazuhiro Shikinaka; Ryuzo Kawamura; Jianping Gong
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, Volume：151, Number：4, First page：444, Last page：444, Dec. 2008
ELSEVIER SCIENCE INC, English, Summary international conference
DOI：https://doi.org/10.1016/j.cbpb.2008.09.062
DOI ID：10.1016/j.cbpb.2008.09.062, ISSN：1096-4959, Web of Science ID：WOS:000261672100019 - 放射光小角X線散乱を用いた配向性を有する微小管集合体の構造評価
敷中一洋; 吉田桃子; 川村隆三; 角五彰; 古川英光; GONG Jian Ping; 増永啓康; 佐々木園; 佐々木園; 増井友美; 小泉智
Volume：57, Number：2 Disk1, 2008
J-Global ID：200902281758612202 - 配向性を有する微小管集合体の作製とその極性評価-ATP駆動型アクティブトランスポーターを目指して-
吉田桃子; 川村隆三; 敷中一洋; 角五彰; 古川英光; GONG Jian Ping
Volume：57, Number：1 Disk1, 2008
J-Global ID：200902299585094908 - ポリカチオンの拡散が誘起する微小管の三次元ネットワーク形成
吉田桃子; 川村隆三; 角五彰; 古川英光; きょう剣萍; きよう剣萍; 長田義仁
Volume：41st, 2007
J-Global ID：200902234616557923 - キネシンによる微小管の動的自己組織化-ATP駆動型アクチュエーターを目指して
川村隆三; 角五彰; 古川英光; GONG Jian Ping; GONG Jian Ping; 長田義仁
Volume：56, Number：1 Disk1, 2007
J-Global ID：200902255747172706
- Soft Actuators 2nd edition (Eds. Kinji Asaka & Hidenori Okuzaki)
Ryuzo Kawamura; Ken-Ichi Sano; Yoshihito Osada, [Contributor], Chapter 40 "Employing Cytoskeletal Treadmilling in Bio-actuators"
Springer, 2019 - Hydrogels of Cytoskeletal Proteins —Preparation, Structure, and Emergent Functions
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano, [Joint work]
Springer International Publishing, Switzerland, 2016 - 設計工学
川村隆三; 佐野健一; 長田義仁, [Joint work]
2013 - 未来材料 11月号
川村隆三; 龔 剣萍, [Joint work]
Nov. 2008 - Formation of motile assembly of microtubules driven by kinesins. Intelligent Materials (M Shahinpoor, H.-J Schneider)
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong, [Joint work], pp. 464–477
RSC Publishing, UK, 2007
- ATP-fueled active network of microtubules as a dynamic environemnt for cells
Ryuzo Kawamura
2nd GLowing Polymer Symposium in KANTO, Nov. 2019, [International conference]
English, Oral presentation - Active network of motor proteins as artificial dynamic microenvironment for cells
Ryuzo Kawamura; Keisuke Meguriya; Tsuyoshi Yokoyama; Daiki Uehara; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
ISAGMSM(The 3rd International Symposium for Advanced Gel Materials & Soft Matters), Jun. 2019, [International conference]
English, Invited oral presentation - Building up a Multi-Channel Flow System to Analyze Cellular
Ryuzo Kawamura; Tsuyoshi Yokoyama; Keisuke Meguriya; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
GelSympo2018, Aug. 2018, [Invited], [International conference]
English, Invited oral presentation - キネシン・微小管の運動集積化と動的細胞環境としての応用
川村 隆三
2017, [Domestic conference]
Japanese, Nominated symposium - キネシン・微小管を用いた運動界面構築と細胞培養基板としての応用
川村 隆三
2016, [Invited], [Domestic conference]
Japanese, Nominated symposium - 微小管でつくる水中駆動マイクロマシン
川村 隆三
2008, [Invited], [Domestic conference]
Japanese, Nominated symposium
■ Research projects
- レーザー技術による機能性分子の秩序構造形成制御
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Oct. 2019 - Mar. 2024
Saitama University
Grant amount(Total)：18460000, Direct funding：14200000, Indirect funding：4260000
Grant number：19KK0128 - Development of a processor with micro-fluctuations driven by motor proteins
JKA (formerly Japan Keirin Association), Research Grant (2-years), Apr. 2021 - Mar. 2023
Saitama University, Principal investigator - 光物理的アプローチによる細胞骨格形成の自在制御技術の開発
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Grant-in-Aid for Challenging Research (Exploratory), Jul. 2020 - Mar. 2023
Saitama University
Grant amount(Total)：6370000, Direct funding：4900000, Indirect funding：1470000
Grant number：20K21117 - Intelligent molecular machinery on high speed molecular display
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Apr. 2019 - Mar. 2023
Hirosaki University
Grant amount(Total)：17420000, Direct funding：13400000, Indirect funding：4020000
Grant number：19H02100 - キネシン駆動型運動界面を利用した細胞の動的力学環境構築
Oct. 2017 - Sep. 2022
Principal investigator
Competitive research funding - Development of novel index for metastatic cancer cell evaluation giving mechanical stimuli by kinesin-driven active matrix
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Apr. 2019 - Mar. 2022
Kawamura Ryuzo, Saitama University
Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
An active matrix which was obtained by crosslinking microtubules to a network and driven by kinesin motor proteins decorated on a solid substrate was developed in this research project toward realization of mechanically active matrix to predict metastatic property of cancer cells with expectation to find a new pathological index. The driving property of the active matrix was found to agitate micro-objects actively in isotropic manner and to generate force up to several nanonewtons. In addition to the generation of microscopic fluctuation by the active matrix, micro-fluidic device was established to realize automatic liquid handling and salvage of the cells exposed to mechanical stimuli on the active matrix and separate them according to the adhesion properties in such the dynamic condition. As the active matrix can induce formation of actin aggregations in metastatic cancer cells, this cell analysis method has potential to allow detection of metastatic cancer cells.
Grant number：19K12775 - 運動界面を利用した転移性がん細胞の変形応答評価系の開発
Oct. 2018 - Mar. 2020
Principal investigator
Competitive research funding - Assembly of active substrate of kinesin and networked-microtubule with ordering
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Apr. 2015 - Mar. 2017
KAWAMURA Ryuzo; NAKABAYASHI Seiichiro; YOSHIKAWAW Hiroshi; KOBAYASHI Naritaka; SUGANUMA Masami, Saitama University
Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
Using an active network of microtubules driven by kinesins on a solid surface, we explored mechanism for coordinative behavior of these motor proteins and applied the constructed system to cells as an active substrate for better understanding of cellular dynamic characters. By patterning the kinesin-coated areas at micrometer and millimeter scale on surfaces, the moving direction of microtubules and the area pattern of active network region were successfully controlled, respectively. With use of the 2D-patterning technique, areas of the active network and the adhered-cells were prepared on the same surface and mechanical stimuli of the fluctuating microtubule network was successfully applied to the neighboring cells. When cells were seeded on this active substrate, unusual deformation of cancer cells after the adhesion was observed as a response to the mechanical stimuli.
Grant number：15K17451 - ポリマー材料中におけるカーボンドットのメカノクロミズム機能探索
2017
Principal investigator
Competitive research funding - がん細胞の硬化に着目した新規転移抑制物質の探索と分子構造の解明（分担）
2016
Competitive research funding