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川村 隆三
| 理工学研究科 物質科学部門 | 准教授 |
| 理学部 基礎化学科 |
- プロフィール：ResearcherID: I-6724-2015
研究者情報
■ 学位■ 研究キーワード
■ 研究分野
■ 研究グループ
■ 経歴
- 2024年04月 - 現在, 埼玉大学, 大学院理工学研究科物質科学部門基礎化学プログラム, 准教授
- 2014年04月 - 2023年03月, 埼玉大学, 大学院理工学研究科戦略的研究部門ライフ・ナノバイオ領域（物質科学部門基礎化学コース兼任）, 助教
- 2012年04月 - 2014年03月, 産業技術総合研究所, バイオメディカル研究部門セルメカニクス研究グループ, 特別研究員
- 2009年01月 - 2012年03月, 理化学研究所, 基幹研究所分子情報生命科学特別研究ユニット, 特別研究員
- 2008年10月 - 2008年12月, 理化学研究所, 基幹研究所分子情報生命科学特別研究ユニット, リサーチアソシエイト
- 2005年04月 - 2008年09月, 北海道大学大学院, 理学研究科, 生物科学専攻
- 2002年04月 - 2005年03月, 東京工業大学大学院, 生命理工学研究科, 生命情報専攻
- 1998年04月 - 2002年03月, 東京工業大学, 生命理工学部, 生物工学科
■ 受賞
業績情報
■ 論文- Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells.
Takahisa Matsuzaki; Mai Fujii; Hayata Noro; Shodai Togo; Mami Watanabe; Masami Suganuma; Shivani Sharma; Naritaka Kobayashi; Ryuzo Kawamura; Seiichiro Nakabayashi; Hiroshi Y Yoshikawa
Proceedings of the National Academy of Sciences of the United States of America, 巻：121, 号：50, 開始ページ：e2412914121, 2024年12月, ［国際誌］
We developed an advanced optical microscope for the simultaneous visualization of membrane fluidity and morphology to define cell adhesion signatures. This microscope combines ratiometric spectral imaging of membrane fluidity and interferometric imaging of membrane morphology. As a preliminary demonstration, we simultaneously visualized the interface between a giant unilamellar vesicle (GUV) and a glass substrate at different temperatures. We identified more fluid regions of the membrane and membrane adhesion sites (conversely, low-fluidic, ordered membrane domains correlate with nonadhered regions). This microscopic system was applied to human breast cancer cell lines with different malignancies; then, we identified adhesion signature of cancer cells: 1) low-fluidic, ordered membrane domains at the cell periphery and 2) large fluidic deviation at the nonadhered region. Inhibition of the cholesterol synthesis pathway suppresses the ordered membrane domains at the cancer cell periphery; thus, high level of cholesterol supports the appearance. Furthermore, an inhibitor of the unsaturated lipid synthesis pathway suppressed the large fluidic deviation at the nonadhered region; variation of unsaturated lipids contributes to heterogeneity of the cancer membrane. Therefore, our advanced optical microscopy enables us to couple membrane physical properties with cell adhesion, leading to definition of adhesion signatures of broad cell types, not just for cancer cells, that regulate life phenomena.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1073/pnas.2412914121
DOI ID：10.1073/pnas.2412914121, PubMed ID：39636859 - In-Situ Observation of DL-Alanine Crystallization from a Laser-Trapped Dense Liquid Droplet as a Heterogeneous Nucleation Site
Hiromasa Niinomi; Hiroshi Y Yoshikawa; Ryuzo Kawamura; Tomoya Yamazaki; Tomoya Oshikiri; Masaru Nakagawa
Chemistry Letters, 2024年05月, ［査読有り］
Abstract
Nucleation from an aqueous solution is an important step in crystallization which controls the physicochemical properties of crystalline materials. Although dense liquid droplets are considered as a precursor of a crystal in the two-step nucleation model, their actual role is unclear. Our in-situ microscopic observations of the crystallization of DL-alanine from a dense liquid droplet trapped by laser tweezers show that the liquid droplets play the role of a substrate facilitating heterogeneous nucleation rather than a precursor of a crystal.
Oxford University Press (OUP), 研究論文（学術雑誌）
DOI：https://doi.org/10.1093/chemle/upae100
DOI ID：10.1093/chemle/upae100, ISSN：0366-7022, eISSN：1348-0715 - Label-free 3D tracking of biological micro-objects using RGB pinholes
Doyeop Kim; Takahisa Matsuzaki; Hiroshi Y. Yoshikawa; Ryuzo Kawamura
Japanese Journal of Applied Physics (Special Issues), 2024年04月, ［査読有り］
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.35848/1347-4065/ad3969
DOI ID：10.35848/1347-4065/ad3969 - Mie-Resonant Nanophotonic-Enhancement of Asymmetry in Sodium Chlorate Chiral Crystallization.
Hiromasa Niinomi; Kazuhiro Gotoh; Naoki Takano; Miho Tagawa; Iori Morita; Akiko Onuma; Hiroshi Y Yoshikawa; Ryuzo Kawamura; Tomoya Oshikiri; Masaru Nakagawa
The journal of physical chemistry letters, 開始ページ：1564, 終了ページ：1571, 2024年02月, ［国際誌］
Studies on chiral spectroscopy have recently demonstrated strong enhancement of chiral light-matter interaction in the chiral near-field of Mie resonance in high-refractive-index dielectric nanostructures by studies on chiral spectroscopy. This situation has motivated researchers to demonstrate effective chiral photosynthesis under a chiral near-field beyond circularly polarized light (CPL) as a chiral source. However, the effectivity of the chiral near-field of Mie resonance for chiral photosynthesis has not been clearly demonstrated. One major challenge is the experimental difficulty in evaluating enantiomeric excess of a trace amount of chiral products synthesized in the near-field. Here, by adopting sodium chlorate chiral crystallization as a phenomenon that includes both synthesis and the amplification of chiral products, we show that crystallization on a Mie-resonant silicon metasurface excited by CPL yields a statistically significant large crystal enantiomeric excess of ∼18%, which cannot be achieved merely by CPL. This result provides implications for efficient chiral photosynthesis in a chiral near-field.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.jpclett.3c03303
DOI ID：10.1021/acs.jpclett.3c03303, PubMed ID：38316420 - Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens.
Takahisa Matsuzaki; Ryuzo Kawamura; Akihisa Yamamoto; Hozumi Takahashi; Mai Fujii; Shodai Togo; Yosuke Yoneyama; Fumihiko Hakuno; Shin-Ichiro Takahashi; Masami Suganuma; Seiichiro Nakabayashi; Shivani Sharma; James K. Gimzewski; Hiroshi Y. Yoshikawa
The Journal of Physical Chemistry Letters, 巻：jz-2023-027675 (acs.jpclett.3c02767), 2024年01月, ［査読有り］
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.jpclett.3c02767
DOI ID：10.1021/acs.jpclett.3c02767 - Spatiotemporal Control of Polymorphic Phase Transition of Glycine Crystals by Three-Dimensional Femtosecond Laser Ablation Processing
Hozumi Takahashi; Yudai Yoshimura; Ryota Murai; Ryuzo Kawamura; Mihoko Maruyama; Masashi Yoshimura; Yusuke Mori; Hiroshi Y. Yoshikawa
The Journal of Physical Chemistry Letters, 巻：15, 号：1, 開始ページ：180, 終了ページ：186, 2023年12月
American Chemical Society (ACS), 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.jpclett.3c02769
DOI ID：10.1021/acs.jpclett.3c02769, ISSN：1948-7185, eISSN：1948-7185 - Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells
Takahisa Matsuzaki; Yuma Kawano; Momoka Horikiri; Yuko Shimokawa; Takashi Yamazaki; Nao Okuma; Hiroyuki Koike; Masaki Kimura; Ryuzo Kawamura; Yosuke Yoneyama; Yasuro Furuichi; Fumihiko Hakuno; Shin-Ichiro Takahashi; Seiichiro Nakabayashi; Satoshi Okamoto; Hiromitsu Nakauchi; Hideki Taniguchi; Takanori Takebe; Hiroshi Y. Yoshikawa
STAR Protocols, 巻：4, 号：3, 開始ページ：102471, 終了ページ：102471, 2023年09月
Elsevier BV, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.xpro.2023.102471
DOI ID：10.1016/j.xpro.2023.102471, ISSN：2666-1667 - Surface-limited reactions for spatial control of kinesin–microtubule motility assays using indirect irradiation of an electron beam
Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biomicrofluidics, 巻：16, 号：6, 開始ページ：064105, 終了ページ：064105, 2022年12月
Gliding of microtubules (MTs) on kinesins has been applied to lab-on-a-chip devices, which enable autonomous transportation and detection of biomolecules in the field of bioengineering. For rapid fabrication and evaluation of the kinesin–MT based devices, optical control techniques have been developed for control of kinesin activity and density; however, use of caged molecules lacks spatial controllability for long-term experiments, and direct irradiations of UV light onto kinesin-coated surfaces are inherently damaging to MTs due to their depth limit since the heights of the kinesin–MT systems are at the tens of a nanometer scale. Considering surface electric fields in electrolytic solutions are shielded at the nanometer scale due to Debye shielding, in this study, we show that fine spatial control of kinesin density and activity is enabled using surface-limited electrochemical reactions induced by indirect irradiations of an electron beam (EB). An EB is indirectly irradiated onto the kinesins through a 100-nm-thick silicon nitride membrane, and the electrons scattered in the membrane can cause localized electrochemical effects to the kinesins. We show that these localized electrochemical effects cause both ablation of kinesins and motility control of kinesin activity by changing the EB acceleration voltage. In particular, the latter is achieved without complete ablation of MTs, though the MTs are indirectly irradiated by the EB. As a demonstration of on-demand control of gliding MTs, we show the accumulation of the MTs on a target area by scanning the EB. The proposed accumulation technique will lead to rapid prototyping of microdevices based on MT–kinesin motility assay systems.
AIP Publishing, 研究論文（学術雑誌）
DOI：https://doi.org/10.1063/5.0124921
DOI ID：10.1063/5.0124921, eISSN：1932-1058 - Mechanical guidance of self-condensation patterns of differentiating progeny
Takahisa Matsuzaki; Yuko Shimokawa; Hiroyuki Koike; Masaki Kimura; Yuma Kawano; Nao Okuma; Ryuzo Kawamura; Yosuke Yoneyama; Yasuro Furuichi; Fumihiko Hakuno; Shin-Ichiro Takahashi; Seiichiro Nakabayashi; Satoshi Okamoto; Hiromitsu Nakauchi; Hideki Taniguchi; Takanori Takebe; Hiroshi Y. Yoshikawa
iScience, 開始ページ：105109, 終了ページ：105109, 2022年09月
Elsevier BV, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.isci.2022.105109
DOI ID：10.1016/j.isci.2022.105109, ISSN：2589-0042 - Intelligent gels – artificial soft tissue for the next era
Ken‐Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Polymer International, 巻：71, 号：6, 開始ページ：616, 終了ページ：629, 2022年06月
Wiley, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/pi.6305
DOI ID：10.1002/pi.6305, ISSN：0959-8103, eISSN：1097-0126 - Chiral Optical Force Generated by a Superchiral Near-Field of a Plasmonic Triangle Trimer as Origin of Giant Bias in Chiral Nucleation: A Simulation Study
Hiromasa Niinomi; Teruki Sugiyama; An-Chieh Cheng; Miho Tagawa; Toru Ujihara; Hiroshi Y. Yoshikawa; Ryuzo Kawamura; Jun Nozawa; Junpei T. Okada; Satoshi Uda
The Journal of Physical Chemistry C, 巻：125, 号：11, 開始ページ：6209, 終了ページ：6221, 2021年03月, ［査読有り］
We previously reported that giant crystal enantiomeric excess (CEE) can be obtained when sodium chlorate (NaClO3) chiral crystallization from a solution is induced by the excitation of localized surface plasmon resonance (LSPR) of a Au triangle trimer nanostructure by a circularly polarized laser. However, the role of the LSPR excitation in the giant CEE remains unclear. In this work, we showed, by finite-difference time-domain analysis of plasmonic near-field, that the magnitude of a chiral optical gradient force originating from the strong superchiral near-field at the Au trimer nanogap on a virtual NaClO3 chiral crystalline cluster is comparable to that of the electric-field gradient force in previous laser-trapping-induced crystallization from unsaturated solution. We revealed that the giant CEE resulted from a difference in the frequency of attachment of chiral crystalline clusters to crystal nuclei or in the local concentration due to chirally biased diffusion rather than enantioselective optical trapping.
American Chemical Society (ACS), 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.jpcc.0c11109
DOI ID：10.1021/acs.jpcc.0c11109, ISSN：1932-7447, eISSN：1932-7455, Web of Science ID：WOS:000635441700026 - Stiffness distribution analysis in indentation depth direction reveals clear mechanical features of cells and organoids by using AFM
Naritaka Kobayashi; Shodai Togo; Takahisa Matsuzaki; Kaede Hashiseko; Ryuzo Kawamura; Masami Suganuma; Seiichiro Nakabayashi; Yosuke Yoneyama; Rie Ouchi; Takanori Takebe; Hiroshi Y Yoshikawa
Applied Physics Express, 巻：13, 号：9, 開始ページ：097001, 終了ページ：097001, 2020年09月, ［査読有り］
Delineating sub-cellular mechanical properties influenced by various cellular components is a challenge in biology in health and disease. We proposed a method to statistically analyze stiffness distribution in indentation depth direction from multiple force curves measured by AFM. The stiffness distributions revealed clear depth-dependent mechanical features of cancer cells and human liver organoids treated with cell/organoid stiffening reagents, which could not be distinguished by conventional analysis that yields averaged stiffness. This work demonstrates that the analytical method is promising for evaluating heterogenous mechanical properties not only of single cell but also of multicellular tissue, and understanding of underlying stiffening mechanisms.
IOP Publishing, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.35848/1882-0786/abaeb5
DOI ID：10.35848/1882-0786/abaeb5, ISSN：1882-0778, eISSN：1882-0786, Web of Science ID：WOS:000565743700001 - Plasmonic Manipulation of Sodium Chlorate Chiral Crystallization: Directed Chirality Transfer via Contact-Induced Polymorphic Transformation and Formation of Liquid Precursor
Hiromasa Niinomi; Teruki Sugiyama; Miho Tagawa; Toru Ujihara; Takashige Omatsu; Katsuhiko Miyamoto; Hiroshi Y. Yoshikawa; Ryuzo Kawamura; Jun Nozawa; Junpei T. Okada; Satoshi Uda
Crystal Growth & Design, 巻：20, 号：8, 開始ページ：5493, 終了ページ：5507, 2020年08月, ［査読有り］
The control of crystallization not only impacts the production of functional crystalline materials or pharmaceuticals but also provides profit to investigate the fundamental mechanism of crystallization. Recently, we have revealed that plasmonic optical tweezers (POT) can "manipulate" the crystallization of a pharmaceutical compound from its aqueous solution by optically trapping molecular clusters, offering a novel strategy to control crystallization. Here we report a variety of unique crystallization phenomena by applying POT to sodium chlorate (NaClO3) chiral crystallization from an aqueous microdroplet loaded on a plasmonic substrate. Plasmon excitation significantly promotes crystallization intermediated by an achiral metastable crystal as a precursor. Also, the direction of the creeping of the resulting chiral crystal can be controlled. By utilizing this phenomenon, we achieved the directed chirality transfer from the chiral crystal to the achiral crystal via a forced contact-induced polymorphic transformation by intentionally making creeping chiral crystal contact with an achiral crystal. Moreover, we captured, by in situ optical microscopic observation, a liquid precursor that intermediates the NaClO3 achiral crystallization for the first time. Our results highlight the plasmonic manipulation of crystallization opening a new door not only to control crystallization but also to investigate the unprecedented fundamental process of crystallization.
American Chemical Society (ACS), 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.cgd.0c00693
DOI ID：10.1021/acs.cgd.0c00693, ISSN：1528-7483, eISSN：1528-7505, Web of Science ID：WOS:000558791100067 - Simultaneous atomic-resolution flexural and torsional imaging in liquid by frequency modulation atomic force microscopy
Megumi Umemoto; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi; Naritaka Kobayashi
JAPANESE JOURNAL OF APPLIED PHYSICS, 巻：59, 2020年06月, ［査読有り］
Liquid-environment frequency modulation atomic force microscopy (FM-AFM) is a powerful nanotool that can investigate phenomena occurring at solid-liquid interfaces in atomic-scale. To extend its function, we have developed FM-AFM system for simultaneous detection of vertical and lateral tip-sample interaction forces in liquid. Owing to tip-sample distance regulation by using a vertical force as a z-feedback signal, we succeeded in detecting a lateral force stably without full contact of a tip to a sample surface. This enabled simultaneous atomic-resolution flexural and torsional imaging in liquid. We demonstrated that the torsional images, which show different contrast pattern with that of the conventional topographic (flexural) images, emphasize specific structures of the topmost surface such as protruding atoms. (C) 2020 The Japan Society of Applied Physics
IOP PUBLISHING LTD, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.35848/1347-4065/ab7479
DOI ID：10.35848/1347-4065/ab7479, ISSN：0021-4922, eISSN：1347-4065, Web of Science ID：WOS:000519637800011 - Quantitative evaluation of the impact of artificial cell adhesion via DNA hybridization on E-cadherin-mediated cell adhesion.
Shodai Togo; Ken Sato; Ryuzo Kawamura; Naritaka Kobayashi; Makoto Noiri; Seiichiro Nakabayashi; Yuji Teramura; Hiroshi Y Yoshikawa
APL bioengineering, 巻：4, 号：1, 開始ページ：016103, 終了ページ：016103, 2020年03月, ［査読有り］, ［国際誌］
Programmable cell adhesion with DNA hybridization is a promising approach for fabricating various tissue architectures without sophisticated instrumentation. However, little is known about how this artificial interaction influences the binding of cell adhesion proteins, E-cadherin. In this work, we designed a planar and fluid lipid membrane displaying E-cadherin and/or single-strand DNA with well-defined densities. Visualization of cells on membranes by fluorescence and interference microscopy revealed cell adhesion to be a two-step process: artificial adhesion by DNA hybridization within a few minutes followed by biological adhesion via cadherin-cadherin binding within hours. Furthermore, we discovered that DNA hybridization can substantially facilitate E-cadherin-mediated cell adhesion. The promotive effect is probably due to the enforced binding between E-cadherin molecules in geometrical confinement between two membranes. Our in vitro model of cell adhesion can potentially be used to design functional synthetic molecules that can regulate cell adhesion via cell adhesion proteins for tissue engineering.
英語
DOI：https://doi.org/10.1063/1.5123749
DOI ID：10.1063/1.5123749, PubMed ID：32002498, PubMed Central ID：PMC6984976 - Selective electrical switching of molecular motors by dynamic virtual cathode
Kenta Hatazawa; Ryuzo Kawamura; Takayuki Hoshino
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 開始ページ：360, 終了ページ：361, 2020年
Environmental and biomedical monitoring bio-applications need the multi-step processing of the chip designs for patterning the biomolecules. This multi-step processing takes a lot of costs to change the biochip design and reconstruct them therefore optimization of the chip function is difficult. So, for reducing processing steps, we propose the on-demand and electrically switched steering of the gliding non-tagged microtubules by the virtual cathode that can generate the electrochemical reactions freely in two dimensional. This method is simpler processing of prototyping than the conventional methods and could be lead to develop the biomedical bio-applications.
研究論文（国際会議プロシーディングス）
Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85098277116&origin=inward
Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85098277116&origin=inward
SCOPUS ID：85098277116 - Pause of the target gliding microtuble on the virtual cathode
Kenta Hatazawa; Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biochemical and Biophysical Research Communications, 巻：514, 号：3, 開始ページ：821, 終了ページ：825, 2019年06月, ［査読有り］
Elsevier BV, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.bbrc.2019.04.200
DOI ID：10.1016/j.bbrc.2019.04.200, ISSN：0006-291X - Start/Stop Motion Control of a Targeted Microtubule Using Virtual Cathode Display
Kenta Hatazawa; Hiroki Miyazako; Ryuzo Kawamura; Takayuki Hoshino
Biochem. Biophys. Res. Comm, 巻：514, 開始ページ：821, 終了ページ：825, 2019年, ［査読有り］ - Reversible surface functionalization of motor proteins for sustainable motility
Keisuke Meguriya; Shiori Kikuchi; Naritaka Kobayashi; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi; Ryuzo Kawamura
Jpn. J. Appl. Phys., 巻：58, 開始ページ：SDDI01, 2019年, ［査読有り］
英語, 研究論文（学術雑誌） - Light-Powered Nanoconverters Cytotoxic to Breast Cancer Cells
Alexandre R. Loukanov; Alexei G. Basnakian; Ryuzo Kawamura; Hibiki Udono; Chavdar K. Filipov; Alena V. Savenka; Todd Fite; Seiichiro Nakabayashi
Journal of Physical Chemistry C, 巻：122, 号：14, 開始ページ：7916, 終了ページ：7924, 2018年04月, ［査読有り］
We designed and fabricated a light-powered nanoconverter for cytotoxicity toward human breast cancer cells. The nanoconverter was made from highly-fluorescent N-doped carbon nanodots (C-dots), which were covalently conjugated to semiconductive hematite quantum dots (Q-dots). The function of the nanoconverter was to transform the absorbed near-infrared (NIR) irradiation into reactive oxygen species (ROS) which would induce cell death. The principle of operation was based on the photosensitizing properties of C-dots, which have a two-photon absorption cross section. They absorbed NIR irradiation with wavelength in the range of 700-800 nm. The adsorbed energy was upconverted to photoluminescence that is emitted as higher-energy visible light with a maximum wavelength of ∼470 nm and transferred to the Q-dot moiety. The process was accompanied by ejection of electrons from the conduction band of Q-dots and by this mechanism, the nanoconverter produced aqueous superoxide anions, which oxidized organics and generated additional ROS. Our nanoconverter exposed in vitro to cultured human breast cancer HCC1954 cells induced light-dependent cell death as measured using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The cell death was minimal when the cells were exposed with C-dots alone or if the nanoconverter was exposed with the cells in dark.
American Chemical Society, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.jpcc.7b11779
Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045390475&origin=inward
Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85045390475&origin=inward
DOI ID：10.1021/acs.jpcc.7b11779, ISSN：1932-7455, eISSN：1932-7455, SCOPUS ID：85045390475 - A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers
Ryuzo Kawamura; Minami Miyazaki; Keita Shimizu; Yuta Matsumoto; Yaron R. Silberberg; Ramachandra Rao Sathuluri; Masumi Iijima; Shun'ichi Ruroda; Futoshi Iwata; Takeshi Kobayashi; Chikashi Nakamura
NANO LETTERS, 巻：17, 号：11, 開始ページ：7117, 終了ページ：7124, 2017年11月, ［査読有り］
Focusing on intracellular targets, we propose a new cell separation technique based on a nano needle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nano needles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nano needles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.nanolett.7b03918
DOI ID：10.1021/acs.nanolett.7b03918, ISSN：1530-6984, eISSN：1530-6992, Web of Science ID：WOS:000415029000091 - Monitoring and extraction of uranium in polluted acid mine drainage by super-paramagnetic nanoparticles coated with carbon nanodots. J. Radioanal. Nucl. Chem., 314, 1149–1159 (2017).
Alexandre Loukanov; Hibiki Udono; Ryo Takakura; Polina Mladenova; Naritaka Kobayashi; Ryuzo Kawamura; Seiichiro Nakabayashi
J. Radioanal. Nucl. Chem., 巻：314, 号：2, 開始ページ：1149, 終了ページ：1159, 2017年11月, ［査読有り］
In this contribution, we designed environmentally friendly magnetic nanoparticles for simultaneously monitoring and efficiently extracting uranium (VI) from polluted acid mine drainage (> 99% of the total uranium amount). The nanoparticles consist of super-paramagnetic core made of Fe3O4, which is chemically coated with highly-fluorescent shell made of carbon nanodots. The small nanoparticle diameter enables good water dispersibility, high mobility in suspension and powerful sorbent activity to U(VI) in the presence of sulfates, bicarbonates, and pH 4.3-4.8. The measured detection limit of uranium (VI) in model solutions of acid mine drainage was found to be around 0.8 ppm.
SPRINGER, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1007/s10967-017-5471-z
DOI ID：10.1007/s10967-017-5471-z, ISSN：0236-5731, eISSN：1588-2780, Web of Science ID：WOS:000413983300070 - Structural change of nonionic surfactant self-assembling at electrochemically controlled HOPG/electrolyte interface
Naritaka Kobayashi; Hirokazu Saitoh; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 巻：799, 開始ページ：444, 終了ページ：450, 2017年08月, ［査読有り］
In this study, we have observed structural change of self-assembling nonionic pentaethylene glycol monododecyl ether (C12E5) surfactant formed on a potential-controlled highly oriented pyrolytic graphite (HOPG) surface by electrochemical frequency modulation atomic force microscopy (EC-FM-AFM). At a negative and a positive potential side with respect to the point of zero charge (pzc), a periodic hemicylindrical structure is formed on the whole surface, while a disordered single-layered thin film is formed at a potential around the pzc. This demonstrates that the electrochemical tuning of the electrode potential enables precise control of the nonionic self assembly structure. We also found that the self-assembly structure is expanded by Na+ ion contained in C12E5 solution. This can be reasonably explained by selective cation binding of a C12E5 molecule like a crown ether. In addition, we estimated a response time of phase transition between the two phases by electrochemical impedance measurement.
ELSEVIER SCIENCE SA, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jelechem.2017.06.046
DOI ID：10.1016/j.jelechem.2017.06.046, ISSN：1572-6657, eISSN：1873-2569, Web of Science ID：WOS:000411847300059 - Kinesin-Driven Active Substrate Giving Stochastic Mechanical Stimuli to Cells for Characterization
Ryuzo Kawamura; Daiki Uehara; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
ACS BIOMATERIALS SCIENCE & ENGINEERING, 巻：2, 号：12, 開始ページ：2333, 終了ページ：2338, 2016年12月, ［査読有り］
We present a new platform to give stochastic mechanical stimuli to cells for their characterization. There nano- and micrometer scaled fluctuations are generated by an engineered motor protein system of kinesin-microtubules (MTs) on a solid surface. Cells have abilities to deform in many ways during homeostatic metabolism, tissue forming processes, cancer developments, and so on. Namely, cells in biological tissues are exposed to noise-like stochastic movements at nano- and micrometer-scales, which mainly come from the mechanical environment surrounding the cells. Although cells seem to have the potential to respond to such types of mechanical stimuli, the influences on cellular behaviors are poorly understood. As a first attempt to verify an effect of noise-like mechanical stimuli in vitro, we prepared a system to give stochastic mechanical stimuli to cells using a technique of in vitro motility assay for a kinesin-MT system. An active substrate was obtained by integrating movements of MTs on a kinesin-coated glass surface via cross-linkage, and stochastic mechanical stimuli at the cell-scale were successfully applied to the seeded cells. There, traveling distances of the cells over one cell length were observed until they started to adhere. When metastatic melanoma cells were exposed to the stochastic mechanical stimuli, unusually long protrusions or extensions of cell bodies were observed. Cellular aggregations were also promoted through the movements on this active substrate which could disturb the landing and enhance the collisions of the cells. This approach giving mechanical stimuli to cells in a stochastic manner at nano- and micrometer-scales might allow us to uncover unknown behaviors of cells, which might contribute to research fields requiring our understanding on the mechanical nature of cells, such as cancer diagnosis and regenerative medicine.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acsbiomaterials.6b00538
DOI ID：10.1021/acsbiomaterials.6b00538, ISSN：2373-9878, Web of Science ID：WOS:000389789200023 - High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells
R. Kawamura; K. Shimizu; Y. Matsumoto; A. Yamagishi; Y. R. Silberberg; M. Iijima; S. Kuroda; K. Fukazawa; K. Ishihara; C. Nakamura
JOURNAL OF NANOBIOTECHNOLOGY, 巻：14, 開始ページ：74, 2016年11月, ［査読有り］
Background: The field of structural dynamics of cytoskeletons in living cells is gathering wide interest, since better understanding of cytoskeleton intracellular organization will provide us with not only insights into basic cell biology but may also enable development of new strategies in regenerative medicine and cancer therapy, fields in which cytoskeleton-dependent dynamics play a pivotal role. The nanoneedle technology is a powerful tool allowing for intracellular investigations, as it can be directly inserted into live cells by penetrating through the plasma membrane causing minimal damage to cells, under the precise manipulation using atomic force microscope. Modifications of the nanoneedles using antibodies have allowed for accurate mechanical detection of various cytoskeletal components, including actin, microtubules and intermediate filaments. However, successful penetration of the nanoneedle through the plasma membrane has been shown to vary greatly between different cell types and conditions. In an effort to overcome this problem and improve the success rate of nanoneedle insertion into the live cells, we have focused here on the fluidity of the membrane lipid bilayer, which may hinder nanoneedle penetration into the cytosolic environment.
Results: We aimed to reduce apparent fluidity of the membrane by either increasing the approach velocity or reducing experimental temperatures. Although changes in approach velocity did not have much effect, lowering the temperature was found to greatly improve the detection of unbinding forces, suggesting that alteration in the plasma membrane fluidity led to increase in nanoneedle penetration.
Conclusions: Operation at a lower temperature of 4 degrees C greatly improved the success rate of nanoneedle insertion to live cells at an optimized approach velocity, while it did not affect the binding of antibodies immobilized on the nanoneedle to vimentins for mechanical detection. As these experimental parameters can be applied to various cell types, these results may improve the versatility of the nanoneedle technology to other cell lines and platforms.
BIOMED CENTRAL LTD, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1186/s12951-016-0226-5
DOI ID：10.1186/s12951-016-0226-5, ISSN：1477-3155, Web of Science ID：WOS:000387731300001 - Intelligent gels: Soft and wet nanomachine of the next era
Ken-Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Electrically Active Materials for Medical Devices, 開始ページ：253, 終了ページ：268, 2016年09月, ［査読有り］
We have developed water-swollen hydrogels with excellent mechanical performances and extremely low frictional properties. These gels exhibited motility under electrical stimulus capable of functioning as the artificial muscles. Novel functional gels including electro-conductive functions and tunable photonic crystal have also developed. Adenosine triphosphate (ATP)-driven biomachines made of protein gels will also be introduced.
Imperial College Press, 英語, 論文集(書籍)内論文
DOI：https://doi.org/10.1142/9781783269877_0018
DOI ID：10.1142/9781783269877_0018, SCOPUS ID：85019842820 - Investigation on nanoscale processes on the BaF2(111) surface in various solutions by frequency modulation atomic force microscopy
Naritaka Kobayashi; Ryuzo Kawamura; Hiroshi Y. Yoshikawa; Seiichiro Nakabayashi
JOURNAL OF APPLIED PHYSICS, 巻：119, 号：21, 開始ページ：214308, 2016年06月, ［査読有り］
In this study, we have directly observed nanoscale processes that occur on BaF2(111) surfaces in various solutions using liquid-environment frequency modulation atomic force microscopy (FM-AFM) with a true atomic resolution. In addition, to investigate atomic-scale mechanisms of crystal growth process of BaF2, we determined a suitable solution for atomic-resolution FM-AFM imaging of the BaF2(111) surface. For undersaturated solutions, the surface is roughened by barium hydroxo complexes in the case of high pH, whereas by dissolution and proton or water molecule adsorption throughout the surface in the case of low pH. On the other hand, for supersaturated solutions, the surface shows two-dimensional nucleation and growth (sigma = 0.1) and three-dimensional crystal growth with tetrahedral structures (sigma = 1), where sigma is the degree of supersaturation. The atomic-resolution imaging of the BaF2(111) surface has been demonstrated in potassium fluoride (KF) and the supersaturated (sigma = 0.1 and 1) solutions, wherein atomically flat terraces are shown at least for about 30 min. Published by AIP Publishing.
AMER INST PHYSICS, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1063/1.4953233
DOI ID：10.1063/1.4953233, ISSN：0021-8979, eISSN：1089-7550, Web of Science ID：WOS:000378923100024 - Biomimetic Functions of Synthetic Polymer Gels
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano
Hydrogels of Cytoskeletal Proteins, 開始ページ：73, 終了ページ：79, 2016年01月
Springer International Publishing, 論文集(書籍)内論文
DOI：https://doi.org/10.1007/978-3-319-27377-8_7
DOI ID：10.1007/978-3-319-27377-8_7 - Design of Polymer Networks Involving a Photoinduced Electronic Transmission Circuit toward Artificial Photosynthesis
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
LANGMUIR, 巻：32, 号：2, 開始ページ：626, 終了ページ：631, 2016年01月, ［査読有り］
Many strategies have been explored to achieve artificial photosynthesis utilizing mediums such as liposomes and supramolecules. Because the photochemical reaction is composed of multiple functional molecules, the surrounding microenvironment is expected to be rationally integrated as observed during photosynthesis in chloroplasts. In this study, photoinduced electronic transmission surrounding the microenvironment of Ru(bpy)3(2+) in a polymer network was investigated using poly(N-isopropylacrylamide-co-Ru(bpy)(3)), poly(acrylamide-co-Ru(bpy)(3)), and Ru(bpy)(3)-conjugated microtubules. Photoinduced energy conversion was evaluated by investigating the effects of (i) Ru(bpy)(3)(2+) immobilization, (ii) polymer type, (iii) thermal energy, and (iv) cross-linking. The microenvironment surrounding copolymerized Ru(bpy)(3)(2+) in poly(N-isopropylacrylamide) suppressed quenching and had a higher radiative process energy than others. This finding is related to the nonradiative process, i.e., photoinduced H-2 generation with significantly higher overall quantum efficiency (13%) than for the bulk solution. We envision that useful molecules will be generated by photoinduced electronic transmission in polymer networks, resulting in the development of a wide range of biomimetic functions with applications for a sustainable society.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.langmuir.5b04326
DOI ID：10.1021/acs.langmuir.5b04326, ISSN：0743-7463, Web of Science ID：WOS:000368563700029 - Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells
Daisuke Matsumoto; Ramachandra Rao Sathuluri; Yoshio Kato; Yaron R. Silberberg; Ryuzo Kawamura; Futoshi Iwata; Takeshi Kobayashi; Chikashi Nakamura
SCIENTIFIC REPORTS, 巻：5, 開始ページ：15325, 2015年10月, ［査読有り］
Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel material and device for the delivery of biomacromolecules into live cells. We attribute the successful results to the unique features of the system, which include high-aspect-ratio, uniform nanoneedles laid across a 2D array, combined with an oscillatory feature, which together allow rapid, forcible and efficient insertion and protein release into thousands of cells simultaneously.
NATURE PUBLISHING GROUP, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1038/srep15325
DOI ID：10.1038/srep15325, ISSN：2045-2322, Web of Science ID：WOS:000362884800001 - Microtubule teardrop patterns
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
SCIENTIFIC REPORTS, 巻：5, 開始ページ：9581, 2015年03月, ［査読有り］
Several strategies for controlling microtubule patterns are developed because of the rigidity determined from the molecular structure and the geometrical structure. In contrast to the patterns in co-operation with motor proteins or associated proteins, microtubules have a huge potential for patterns via their intrinsic flexural rigidity. We discover that a microtubule teardrop pattern emerges via self-assembly under hydrodynamic flow from the parallel bundles without motor proteins. In the growth process, the bundles ultimately bend according to the critical bending curvature. Such protein pattern formation utilizing the intrinsic flexural rigidity will provide broad understandings of self-assembly of rigid rods, not only in biomolecules, but also in supramolecules.
NATURE PUBLISHING GROUP, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1038/srep09581
DOI ID：10.1038/srep09581, ISSN：2045-2322, Web of Science ID：WOS:000351948000001 - Specialized nanoneedles for intracellular analysis
Ryuzo Kawamura; Yaron R. Silberberg; Chikashi Nakamura
Nanobiosensors and Nanobioanalyses, 開始ページ：291, 終了ページ：303, 2015年01月, ［査読有り］
Here, we introduce a novel approach to the detection of intracellular molecules by measuring direct interactions with an ultrathin probe, i.e. nanoneedle, which is mounted on an atomic force microscope (AFM). Standard AFM probes were sharpened, using a focused ion beam (FIB), to form high-aspect-ratio nanoneedles, which were then specifically functionalized and inserted into live cells. The insertion could be precisely detected using the resulted force-distance AFM curves, and no effect on cell viability was observed, even after repeated insertions. In addition, thanks to the high sensitivity of the AFM, distinct intermolecular unbinding events could be analyzed, which provided real-time information on the cytoskeleton state of the live cell. Following specific coatings and functionalization of the nanoneedles, various intracellular molecules could be detected and even inserted into live cells. The results presented here demonstrate the delivery of DNA vectors and the detection of mRNA and cytoskeletal proteins in live cells. Further advances in this technology, such as new developments in molecular functionalization options and improvements in the scale and accuracy of force measurements, will open possible new fields and applications for this diverse and powerful tool.
Springer Japan, 英語, 論文集(書籍)内論文
DOI：https://doi.org/10.1007/978-4-431-55190-4_14
DOI ID：10.1007/978-4-431-55190-4_14, SCOPUS ID：84955671680 - Effect of microtubule polymerization on photoinduced hydrogen generation
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
CHEMICAL COMMUNICATIONS, 巻：51, 号：58, 開始ページ：11607, 終了ページ：11610, 2015年, ［査読有り］
Herein we report a novel reaction field for photoinduced H-2 generation by using microtubules as a medium. By controlling the tubulin/microtubule hierarchical structure, synergistic effects by which the Ru(bpy)3(2+)-conjugated microtubule network causes suppression of energy loss by collision are clarified.
ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1039/c5cc02914g
DOI ID：10.1039/c5cc02914g, ISSN：1359-7345, eISSN：1364-548X, Web of Science ID：WOS:000357618200015 - Kosuke Okeyoshi, Ryuzo Kawamura, Ryo Yoshida, Yoshihito Osada
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
Chem. Comm., 巻：51, 開始ページ：11607, 終了ページ：11610, 2015年, ［査読有り］
英語, 研究論文（学術雑誌） - Effect of microtubules hierarchy on photoinduced hydrogen generation and application to artificial photosynthesis
Kosuke Okeyoshi; Kawamura Ryuzo; Ryo Yoshida; Yoshihito Osada
Journal of Fluid Mechanics, 巻：1621, 2014年11月, ［査読有り］
Several strategies have been explored from viewpoint of biomimetics to accomplish artificial photosynthesis by using macromolecules as a medium such as liposomes, supramolecules, and hydrogels.1 Differing from disordered solution systems in which multiple components such as photosensitizer and catalytic nanoparticle are diffusively mixed, the photochemical reactions occur efficiently in medium due to maintenance of the dipersibility of the components and specific molecular arrangement. Here we attempt to clarify the effect of medium hierarchy for photoinduced electronic transmission among multiple components. By conjugating each component on tubulin and integrating them via self-assembly to microtubules, ideal component arrangements with optimum distance for the electronic transmission will be possible.
Cambridge University Press, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1557/opl.2014.174
DOI ID：10.1557/opl.2014.174, ISSN：1469-7645, SCOPUS ID：84908554921 - Measurement of cell adhesion force by vertical forcible detachment using an arrowhead nanoneedle and atomic force microscopy
Seunghwan Ryu; Yui Hashizume; Mari Mishima; Ryuzo Kawamura; Masato Tamura; Hirofumi Matsui; Michiya Matsusaki; Mitsuru Akashi; Chikashi Nakamura
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 巻：451, 号：1, 開始ページ：107, 終了ページ：111, 2014年08月, ［査読有り］
The properties of substrates and extracellular matrices (ECM) are important factors governing the functions and fates of mammalian adherent cells. For example, substrate stiffness often affects cell differentiation. At focal adhesions, clustered-integrin bindings link cells mechanically to the ECM. In order to quantitate the affinity between cell and substrate, the cell adhesion force must be measured for single cells. In this study, forcible detachment of a single cell in the vertical direction using AFM was carried out, allowing breakage of the integrin-substrate bindings. An AFM tip was fabricated into an arrowhead shape to detach the cell from the substrate. Peak force observed in the recorded force curve during probe retraction was defined as the adhesion force, and was analyzed for various types of cells. Some of the cell types adhered so strongly that they could not be picked up because of plasma membrane breakage by the arrowhead probe. To address this problem, a technique to reinforce the cellular membrane with layer-by-layer nanofilms composed of fibronectin and gelatin helped to improve insertion efficiency and to prevent cell membrane rupture during the detachment process, allowing successful detachment of the cells. This method for detaching cells, involving cellular membrane reinforcement, may be beneficial for evaluating true cell adhesion forces in various cell types. (C) 2014 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.bbrc.2014.07.078
DOI ID：10.1016/j.bbrc.2014.07.078, ISSN：0006-291X, eISSN：1090-2104, Web of Science ID：WOS:000340864200017 - Employing cytoskeletal treadmilling in bio-actuator
Ken-Ichi Sano; Ryuzo Kawamura; Yoshihito Osada
Soft Actuators: Materials, Modeling, Applications, and Future Perspectives, 巻：9784431547679, 開始ページ：489, 終了ページ：497, 2014年07月, ［査読有り］
In this chapter, we describe treadmilling bio-actuators. The principle of treadmilling actuator is, filamentous protein complex formation by actin or tubulin accompanying a sequence of nucleotide triphosphate hydrolysis is to alter the critical concentration of polymerization at the two ends of the filament. Recently, we have succeeded in the creation of hydrogels which autonomously oscillate owing to the treadmilling of actin or tubulin. These hydrogels have great potential as bio-actuators because they are easy to make on a centimeter scale.
Springer Japan, 英語, 論文集(書籍)内論文
DOI：https://doi.org/10.1007/978-4-431-54767-9_35
DOI ID：10.1007/978-4-431-54767-9_35, SCOPUS ID：84930712258 - Effect of microtubules hierarchy on photoinduced hydrogen generation and application to artificial photosynthesis
Kosuke Okeyoshi; Kawamura Ryuzo; Ryo Yoshida; Yoshihito Osada
Materials Research Society Symposium Proceedings, 巻：1621, 2014年, ［査読有り］
Several strategies have been explored from viewpoint of biomimetics to accomplish artificial photosynthesis by using macromolecules as a medium such as liposomes, supramolecules, and hydrogels.1 Differing from disordered solution systems in which multiple components such as photosensitizer and catalytic nanoparticle are diffusively mixed, the photochemical reactions occur efficiently in medium due to maintenance of the dipersibility of the components and specific molecular arrangement. Here we attempt to clarify the effect of medium hierarchy for photoinduced electronic transmission among multiple components. By conjugating each component on tubulin and integrating them via self-assembly to microtubules, ideal component arrangements with optimum distance for the electronic transmission will be possible. Copyright © Materials Research Society 2014.
Materials Research Society, 英語, 研究論文（国際会議プロシーディングス）
DOI：https://doi.org/10.1557/opl.2014.174
DOI ID：10.1557/opl.2014.174, ISSN：0272-9172, SCOPUS ID：84897974976 - Controlling the bias of rotational motion of ring-shaped microtubule assembly
Shoki Wada; Arif Md; Rashedul Kabir; Ryuzo Kawamura; Masaki Ito; Daisuke Inoue; Kazuki Sada; Akira Kakugo
Biomacromolecules, 巻：16, 開始ページ：374, 終了ページ：378, 2014年, ［査読有り］
英語, 研究論文（学術雑誌） - Chemically cross-linked microtubule assembly shows enhanced dynamic motions on kinesins
Ryuzo Kawamura; Ken-Ichi Sano; Kuniharu Ijiro; Yoshihito Osada
RSC ADVANCES, 巻：4, 号：62, 開始ページ：32953, 終了ページ：32959, 2014年, ［査読有り］
Biomolecular motors have cooperative functions that facilitate various important cellular functions such as signaling and deformation. However, the multiple-molecular behavior is poorly understood when compared with the well-studied behavior of single molecules. Kinesin and microtubules (MTs) are a combination of motor and rail proteins that have been reported to show cooperativity in moving patterns. Here, we report that cross-linking of isotropic MTs enhances the movement of the local network temporally, which can elicit the effective conveyance of 5 mu m diameter silica microbeads. The average velocity of the beads reached 143 nm s(-1) for over 15 min, which is 2.7 times higher than the beads on kinesin-driven MTs without cross-links. Although the velocity of the beads fluctuated over the time course, the maximum velocity calculated from 5 s displacement reached 1.2 mu m s(-1), which was ca. 3-fold higher than the intrinsic driving velocity of kinesins, i.e., the sliding velocity of the MTs without cross-links. This technique provides such an enhanced movement by simply cross-linking the isotropic MTs, but without specific control or alignment over network polarity. The enhancement in the local motion of the MTs is discussed in terms of the "mechanochemical" effect coupled between dynamically stored elastic energies in the MT network and the collective motion of MTs driven by an ATP-fueled kinesin motor.
ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1039/c4ra04491f
DOI ID：10.1039/c4ra04491f, ISSN：2046-2069, Web of Science ID：WOS:000341083400049 - Thermo- and photo-enhanced microtubule formation from Ru(bpy)(3)(2+)-conjugated tubulin
Kosuke Okeyoshi; Ryuzo Kawamura; Ryo Yoshida; Yoshihito Osada
JOURNAL OF MATERIALS CHEMISTRY B, 巻：2, 号：1, 開始ページ：41, 終了ページ：45, 2014年, ［査読有り］
Ru(bpy)(3)(2+)-conjugated tubulin is able to substantially enhance polymerization to form microtubules with increased rate at lower temperatures. Additionally, the polymerization is enhanced by photo-irradiation and the possible mechanism is discussed focusing on the photo-thermal energy conversion.
ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1039/c3tb21242d
DOI ID：10.1039/c3tb21242d, ISSN：2050-750X, eISSN：2050-7518, Web of Science ID：WOS:000327607100005 - Detection of microtubules in vivo using antibody-immobilized nanoneedles
Yaron R. Silberberg; Ryuzo Kawamura; Seunghwan Ryu; Kyoko Fukazawa; Kazuhiko Ishihara; Chikashi Nakamura
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 巻：117, 号：1, 開始ページ：107, 終了ページ：112, 2014年01月, ［査読有り］
We present here an alternative, force-based measurement method for the detection of intracellular cytoskeletal proteins in the live cell. High aspect ratio nanoneedles of 200 nm in diameter were functionalized with anti-tubulin antibodies and inserted, using an atomic force microscope (AFM), into live NIH3T3 cells, without affecting cell viability. Force curves were recorded during insertion and evacuation of nanoneedles from the cells, and used to analyse intracellular interactions of the nanoneedles with the microtubule cytoskeleton during evacuation from the cell. Disruption of microtubules led to a correlated time-dependent decrease in the measured intracellular binding forces, pointing to the high-sensitivity and high-specificity of this detection method. This analytical technique allows for real-time evaluation of the microtubule network in the live cell, without the need to use potentially harmful molecular markers as do conventional detection methods, and may prove beneficial in the diagnosis and investigation of cytoskeleton-associated diseases. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
SOC BIOSCIENCE BIOENGINEERING JAPAN, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jbiosc.2013.06.019
DOI ID：10.1016/j.jbiosc.2013.06.019, ISSN：1389-1723, eISSN：1347-4421, Web of Science ID：WOS:000333071300018 - Nanoneedle insertion into the cell nucleus does not induce double-strand breaks in chromosomal DNA
Seunghwan Ryu; Ryuzo Kawamura; Ryohei Naka; Yaron R. Silberberg; Noriyuki Nakamura; Chikashi Nakamura
Journal of Bioscience and Bioengineering, 巻：116, 号：3, 開始ページ：391, 終了ページ：396, 2013年09月, ［査読有り］
An atomic force microscope probe can be formed into an ultra-sharp cylindrical shape (a nanoneedle) using micro-fabrication techniques such as focused ion beam etching. This nanoneedle can be effectively inserted through the plasma membrane of a living cell to not only access the cytosol, but also to penetrate through the nuclear membrane. This technique shows great potential as a tool for performing intranuclear measurements and manipulations. Repeated insertions of a nanoneedle into a live cell were previously shown not to affect cell viability. However, the effect of nanoneedle insertion on the nucleus and nuclear components is still unknown. DNA is the most crucial component of the nucleus for proper cell function and may be physically damaged by a nanoneedle. To investigate the integrity of DNA following nanoneedle insertion, the occurrence of DNA double-strand breaks (DSBs) was assessed. The results showed that there was no chromosomal DNA damage due to nanoneedle insertion into the nucleus, as indicated by the expression level of γ-H2AX, a molecular marker of DSBs. © 2013 The Society for Biotechnology, Japan.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jbiosc.2013.03.022
DOI ID：10.1016/j.jbiosc.2013.03.022, ISSN：1389-1723, PubMed ID：23643618, SCOPUS ID：84881101246 - Controlled cell adhesion using a biocompatible anchor for membrane-conjugated bovine serum albumin/bovine serum albumin mixed layer
Ryuzo Kawamura; Mari Mishima; Seunghwan Ryu; Yu Arai; Motomu Okose; Yaron R. Silberberg; Sathuluri Ramachandra Rao; Chikashi Nakamura
Langmuir, 巻：29, 号：21, 開始ページ：6429, 終了ページ：6433, 2013年05月, ［査読有り］
DOI：https://doi.org/10.1021/la4012229
DOI ID：10.1021/la4012229, ISSN：0743-7463, PubMed ID：23639009 - 2P179 AFMを用いた強制剥離による細胞接着力の評価(12.細胞生物的課題,ポスター,日本生物物理学会年会第51回(2013年度))
Mishima Mari; Kawamura Ryuzo; Okada Tomoko; Nakamura Chikashi
生物物理, 巻：53, 号：1, 開始ページ：S188, 2013年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.53.S188_5
DOI ID：10.2142/biophys.53.S188_5, CiNii Articles ID：110009819668 - Microtuhular teardrop patterning and the growing process
Kosuke Okeyoshi; Kawamura Ryuzo; Yoshihito Osada
Materials Research Society Symposium Proceedings, 巻：1504, 開始ページ：22, 終了ページ：28, 2013年, ［査読有り］
Here we show that microtubular bundles bend flexibly under a hydrodynamic flow to form teardrop patterns, [n a highly concentrated microtubular solution, patterns of same-sized teardrops form according to the maximum critical curvature, which is determined by the specific rigidity of the microtubules. Our understanding is that these micropatterns grow when microtubular bundles with hydrodynamic flow energy are converted into stable teardrop patterns as a higher structure. This conversion is generated by the combined effect of multiple kinds of energy, including heat and hydrodynamic flow, as well as life systems. These self-generating patterns in a spatio-temporal stream are reminiscent of what the artist Edward Munch called a scream of nature. We also envision that microtubular pattering with hierarchical structure will broaden the potential application of these geometrical structures and guide biomimetic material engineering towards areas such as integrated energy conversion, soft material patterning, and living signal transduction. © 2013 Materials Research Society.
Materials Research Society, 英語, 研究論文（国際会議プロシーディングス）
DOI：https://doi.org/10.1557/opl.2013.243
DOI ID：10.1557/opl.2013.243, ISSN：0272-9172, SCOPUS ID：84899871585 - Controlled cell adhesion using a BAM-BSA/BSA mixed layer
Ryuzo Kawamura; Mari Mishima; Seunghwan Ryu; Yu Arai; Motomu Okose; Yaron R. Silberberg; Sathuluri Ramachandra Rao; Chikashi Nakamura
Langmuir, 巻：29, 開始ページ：6429, 終了ページ：6433, 2013年, ［査読有り］
英語, 研究論文（学術雑誌） - Nanopattern Fabrication of Gold on Hydrogels and Application to Tunable Photonic Crystal
Naonobu Shimamoto; Yoshito Tanaka; Hideyuki Mitomo; Ryuzo Kawamura; Kuniharu Ijiro; Keiji Sasaki; Yoshihito Osada
ADVANCED MATERIALS, 巻：24, 号：38, 開始ページ：5243, 終了ページ：5248, 2012年10月, ［査読有り］
WILEY-V C H VERLAG GMBH, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/adma.201201522
DOI ID：10.1002/adma.201201522, ISSN：0935-9648, eISSN：1521-4095, Web of Science ID：WOS:000309176800013 - Nematic growth of microtubules that changed into giant spiral structure through partial depolymerization and subsequent dynamic ordering
Kiyotaka Shigehara; Hiroki Kudoh; Saori Mori; Yoshiki Tamura; Akira Kakugo; Ryuzo Kawamura; Hidemitsu Furukawa; Jian Ping Gong; Hiroyasu Masunaga; Tomomi Masui; Satoshi Koizumi; Kazuhiro Shikinaka
SOFT MATTER, 巻：8, 号：45, 開始ページ：11544, 終了ページ：11551, 2012年, ［査読有り］
In a long capillary cell with temperature gradient, tubulin dimers with alpha and beta subunits polymerized according to the preferential polarity, i.e., the anisotropic spiral addition of the dimers to the beta-terminated "plus end" dominated the formation of microtubules. As the result, the helical hollow cylinders generated the oriented nematic liquid crystalline structure with centimeter-length. In the next stage, where microtubules were under the partial polymerization/depolymerization equilibrium due to the concentration fluctuation, the dynamic rearrangement of microtubules such as their shortening (depolymerization) and subsequent tilting of orientation axis caused the structural change from the oriented nematic liquid crystalline structure to some giant spiral structure which was subjected by the ordered dipole and the helical chirality of microtubules.
ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1039/c2sm26675j
DOI ID：10.1039/c2sm26675j, ISSN：1744-683X, eISSN：1744-6848, Web of Science ID：WOS:000310829800018 - Formation of motile assembly of microtubules driven by kinesins
Ryuzo Kawamura; Akira Kakugo; Kazuhiro Shikinaka; Yoshihito Osada; Jian Ping Gong
SMART MATERIALS AND STRUCTURES, 巻：20, 号：12, 開始ページ：124007, 2011年12月, ［査読有り］
Microtubule (MT) and kinesin are rail and motor proteins that are involved in various moving events of eukaryotic cells in natural systems. In vitro, the sliding motion of microtubules (rail) can be reproduced on a kinesin (motor protein)-coated surface coupled with adenosine triphosphate (ATP) hydrolysis, which is called a `motility assay'. Based on this technique, a method was recently established to form MT assemblies by an active self-assembly (AcSA) process, in which MTs are crosslinked during a sliding motion on a kinesin-coated surface. Streptavidin (ST) was employed as glue to crosslink biotin-labeled MTs. Various shapes, sizes, and motilities were formed with the AcSA MT assemblies, depending on the initial conditions. In this paper, we briefly review our recent work on the formation of MT assemblies on a kinesin-coated surface.
IOP PUBLISHING LTD, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1088/0964-1726/20/12/124007
DOI ID：10.1088/0964-1726/20/12/124007, ISSN：0964-1726, eISSN：1361-665X, Web of Science ID：WOS:000298140200008 - Self-Repairing Filamentous Actin Hydrogel with Hierarchical Structure
Ken-Ichi Sano; Ryuzo Kawamura; Taiki Tominaga; Naoko Oda; Kuniharu Ijiro; Yoshihito Osada
BIOMACROMOLECULES, 巻：12, 号：12, 開始ページ：4173, 終了ページ：4177, 2011年12月, ［査読有り］
A chemically cross-linked filamentous actin (F-actin) gel consisting of globular actin (G-actin) as repeating units was prepared. The F-actin gel was cross-linked by covalent bonds, and the main chain is represented by the self assembly of G-actin with a high-ordered hierarchical structure. The gel. exhibited good mechanical performance with a storage modulus >1 kPa and undergoes reversible sol-gel transitions. in response to changes in the salt concentration (chemical induced sol-gel transition) as well as to shear strain (mechanical-induced sol-gel transition). Therefore, the gel exhibits self repairing ability through dynamic polymerization and depolymerization across the structure hierarchies under repeated shear stress.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/bm2009922
DOI ID：10.1021/bm2009922, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000297782100001 - Thermoresponsive Microtubule Hydrogel with High Hierarchical Structure
Ken-Ichi Sano; Ryuzo Kawamura; Taiki Tominaga; Hiromichi Nakagawa; Naoko Oda; Kuniharu Ijiro; Yoshihito Osada
BIOMACROMOLECULES, 巻：12, 号：5, 開始ページ：1409, 終了ページ：1413, 2011年05月, ［査読有り］
A thermoresponsive 3D microtubule hydrogel (MT gel) was prepared by simultaneous polymerization and chemical cross-linking of tubulins. The main chain of this gel is composed of cross-linked MTs, which consists of a cylindrical assembly of tubulin covalently connected by polyethylene glycol. This gel, which contains 10 mg/mL of tubulin, exhibits a storage modulus G' as high as 1 x 10(3), which is 10 times higher than the loss modulus G '' over a wide range of frequencies. The MT gel exhibits a reversible sol gel transition by temperature changes at 4-37 degrees C via depolymerization and polymerization of the MT network. Notable effects of the presence of the cross-linkage on the process of polymerization and depolymerization of tubulin were experimentally observed, and the role of the cross-linkage was discussed.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/bm101578x
DOI ID：10.1021/bm101578x, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000290246400002 - 3J1458 化学架橋による微小管のネットワークのキネシンによる運動性(バイオエンジニアリング2,第49回年会講演予稿集)
Kawamura Ryuzo; Sano Ken-ichi; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
生物物理, 巻：51, 開始ページ：S143, 2011年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.51.S143_2
DOI ID：10.2142/biophys.51.S143_2, CiNii Articles ID：110008903569 - Dynamic self-organization and polymorphism of microtubule assembly through active interactions with kinesin
Yoshiki Tamura; Ryuzo Kawamura; Kazuhiro Shikinaka; Akira Kakugo; Yoshihito Osada; Jian Ping Gong; Hiroyuki Mayama
SOFT MATTER, 巻：7, 号：12, 開始ページ：5654, 終了ページ：5659, 2011年, ［査読有り］
In this study, we show that the energy-dissipative active self-assembly of microtubules (MTs) via a kinesin-based motility system produces various MT assemblies such as bundle-, network-, and ring-shaped structures depending on the initial conditions. Structural polymorphism of the MT assembly is depicted through phase diagrams, and morphogenesis of the MT assembly is discussed based on the following factors: binding force between MTs and motility-driving force from kinesins. This study provides new insights into the energy-dissipative dynamic self-organization of biological systems.
ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1039/c1sm05413a
DOI ID：10.1039/c1sm05413a, ISSN：1744-683X, eISSN：1744-6848, Web of Science ID：WOS:000291354200025 - Microtubule bundle formation driven by ATP: the effect of concentrations of kinesin, streptavidin and microtubules
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong
NANOTECHNOLOGY, 巻：21, 号：14, 開始ページ：145603, 2010年04月, ［査読有り］
Recently, a method was established for the formation of microtubule (MT) assemblies by an active self-organization (AcSO) process, in which MTs were crosslinked during sliding motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate (ATP) hydrolysis. Streptavidin (ST) was the glue used to crosslink biotin-labeled MTs. Although most of the MT assemblies were in the bundle form, they varied in size, shape and motility, depending on the initial conditions used. In this paper, we systematically examined the effects of the concentrations of kinesin, ST and MT on the formation of MT bundles under the initial conditions of the process.
IOP PUBLISHING LTD, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1088/0957-4484/21/14/145603
DOI ID：10.1088/0957-4484/21/14/145603, ISSN：0957-4484, eISSN：1361-6528, Web of Science ID：WOS:000275652200029 - 1P155 1D1505 Autonomous oscillation in mechanical property of actin gel(Muscle,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)
Kawamura Ryuzo; Sano Ken-Ichi; Tominaga Taiki; Ijiro Kuniharu; Osada Yoshihito
生物物理, 巻：50, 号：2, 開始ページ：S46, 2010年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.50.S46_5
DOI ID：10.2142/biophys.50.S46_5, CiNii Articles ID：110008102391 - 1P156 Reversible gel-sol transition of covalently cross-linked actin hydrogel(Muscle,The 48th Annual Meeting of the Biophysical Society of Japan)
Sano Ken-Ichi; Kawamura Ryuzo; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
生物物理, 巻：50, 号：2, 開始ページ：S46, 終了ページ：S47, 2010年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.50.S46_6
DOI ID：10.2142/biophys.50.S46_6, CiNii Articles ID：110008102392 - Selective Formation of a Linear-Shaped Bundle of Microtubules
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong
LANGMUIR, 巻：26, 号：1, 開始ページ：533, 終了ページ：537, 2010年01月, ［査読有り］
By using rigid microtubules (MTs) prepared by polymerization with guanylyl-(alpha, beta)-methylene-diphosphonate GMPCPP, giant straight-shaped MT bundles were selectively obtained through a dynamic self-assembly process. We demonstrate the effect of the rigidity oil the shape and motility of MT bundle composed of GMPCPP-polymerized MTs (GMPCPP-MTs) compared with control MTs that were polymerized with GTP and stabilized with paclitaxel.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/la902197f
DOI ID：10.1021/la902197f, ISSN：0743-7463, Web of Science ID：WOS:000272937500072 - Formation of Well-Oriented Microtubules with Preferential Polarity in a Confined Space under a Temperature Gradient
Akira Kakugo; Yoshiki Tamura; Kazuhiro Shikinaka; Momoko Yoshida; Ryuzo Kawamura; Hidemitsu Furukawa; Yoshihito Osada; Jian Ping Gong
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 巻：131, 号：50, 開始ページ：18089, 終了ページ：18095, 2009年12月, ［査読有り］
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/ja901538n
DOI ID：10.1021/ja901538n, ISSN：0002-7863, Web of Science ID：WOS:000273615400031 - 3P-266 微小管による温度可逆的なハイドロゲルの創製(バイオエンジニアリング,第47回日本生物物理学会年会)
Kawamura Ryuzo; Sano Ken-Ichi; Kunita Istuki; Tominaga Taiki; Oda Naoko; Ijiro Kuniharu; Osada Yoshihito
生物物理, 巻：49, 開始ページ：S195, 2009年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.49.S195_3
DOI ID：10.2142/biophys.49.S195_3, CiNii Articles ID：110008102120 - (学位論文) キネシンによる微小管の動的自己組織化に関する研究
川村 隆三
北海道大学, 2008年12月, ［査読有り］
英語, 学位論文（博士） - Ring-shaped assembly of microtubules shows preferential counterclockwise motion
Ryuzo Kawamura; Akira Kakugo; Kazuhiro Shikinaka; Yoshihito Osada; Jian Ping Gong
BIOMACROMOLECULES, 巻：9, 号：9, 開始ページ：2277, 終了ページ：2282, 2008年09月, ［査読有り］
In this paper, we reveal that microtubules (MTs), reconstructed from tubulin in vitro in the presence of guanosine-5'-triphosphate (GTP), have a ring or spiral shape on a motor protein-fixed surface, and these MTs show biased motion in the counterclockwise direction. By cross-linking these MTs during the sliding motion, we obtained large ring-shaped MT assemblies, 1 similar to 12.6 mu m in diameter. The ratio of the rings rotating in the counterclockwise direction to those rotating in the clockwise direction was approximately 3/1. Under optimized conditions, the ratio was as high as 14/1. Thus, We Successfully obtained aggregated MTs with a large hierarchic structure that shows a preferential motion, through a dynamic process in vitro.
AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/bm800639w
DOI ID：10.1021/bm800639w, ISSN：1525-7797, eISSN：1526-4602, Web of Science ID：WOS:000259033700001 - Integration of Biomolecular Motors: Toward an ATP Fueled Soft Moving System
Kazuhiro Shikinaka; Ryuzo Kawamura; Akira Kakugo; Hidemitsu Furukawa; Jian Ping Gong; Yoshihito Osada
Polymer Preprints, Japan, 巻：57, 開始ページ：106, 2008年
英語, 研究論文（学術雑誌） - 2P161 キネシンによる微小菅の動的自己組織化 : ATP駆動型アクチュエーターを目指して(分子モーター,ポスター発表,第45回日本生物物理学会年会)
川村 隆三; 角五 彰; 古川 英光; Gong Jian Ping; 長田 義仁
生物物理, 巻：47, 開始ページ：S153, 2007年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.47.S153_2
DOI ID：10.2142/biophys.47.S153_2, CiNii Articles ID：110006562252 - Transduction of NeuroD2 protein induced neural cell differentiation
Tomohide Noda; Ryuzo Kawamura; Hisakage Funabashi; Masayasu Mie; Eiry Kobatake
JOURNAL OF BIOTECHNOLOGY, 巻：126, 号：2, 開始ページ：230, 終了ページ：236, 2006年11月, ［査読有り］
NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta 100-115) (lack of NLS), ND2(Delta 123-134) (lack of basic) and ND2(Delta 100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein. (c) 2006 Elsevier B.V. All rights reserved.
ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jbiotec.2006.04.021
DOI ID：10.1016/j.jbiotec.2006.04.021, ISSN：0168-1656, eISSN：1873-4863, Web of Science ID：WOS:000241783300012 - Doxorubicin encapsulation and diffusional release from stable, polymeric, hydrogel nanoparticles
Dimitrios Missirlis; Ryuzo Kawamura; Nicola Tirelli; Jeffrey A. Hubbell
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 巻：29, 号：2, 開始ページ：120, 終了ページ：129, 2006年10月, ［査読有り］
We have recently described the preparation of stable, polymeric nanoparticles, composed of poly(ethylene glycol) and poloxamer 407 (Pluronic (R) F127), prepared via inverse emulsion photopolymerization. In the present study we report on the performance of this novel colloidal system as a controlled delivery system for small hydrophobic drugs. Successful encapsulation of doxorubicin occurred through hydrophobic interactions, taking advantage of particle nanoarchitecture. Loadings of up to 8.7 wt.% were achieved with a reproducible, fast, solvent evaporation procedure. In vitro drug release, monitored by fluorescence spectrometry and HPLC, revealed a minor burst (approximately 10% at 37 degrees C) and sustained, diffusional release for over 1 week; furthermore, drug encapsulation significantly delayed doxorubicin degradation kinetics. (c) 2006 Published by Elsevier B.V.
ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.ejps.2006.06.003
DOI ID：10.1016/j.ejps.2006.06.003, ISSN：0928-0987, Web of Science ID：WOS:000241372100004
- ポリマー材料中におけるカーボンドットのメカノクロミズム機能探索
川村隆三; LOUKANOV Alexandre
マツダ財団研究報告書(科学技術振興関係), 巻：31, 2019年
ISSN：1344-0497, J-Global ID：201902236196531935 - バーチャル電極ディスプレイの微小管滑走アッセイでの局所性評価
畑澤研太; 宮廻裕樹; 川村隆三; 星野隆行; 星野隆行
応用物理学会春季学術講演会講演予稿集(CD-ROM), 巻：66th, 2019年
J-Global ID：201902248990834557 - DNA-PEG脂質を介した細胞接着機構の解明-細胞組織形成の自在制御に向けて-
東郷祥大; 佐藤健; 川村隆三; 小林成貴; 中林誠一郎; 寺村裕治; 吉川洋史
日本化学会春季年会講演予稿集(CD-ROM), 巻：98th, 2018年
J-Global ID：201802233641288888 - DNA hybridizationが細胞接着に与える影響の解明-細胞組織形成の人為的誘導に向けて-
佐藤健; 寺村裕治; 川村隆三; 小林成貴; 中林誠一郎; 吉川洋史
応用物理学会春季学術講演会講演予稿集(CD-ROM), 巻：64th, 2017年
J-Global ID：201702276065767041 - DNA-PEG脂質を用いた細胞接着ダイナミクスの解明
佐藤健; 寺村裕治; 川村隆三; 中林誠一郎; 吉川洋史
日本化学会春季年会講演予稿集(CD-ROM), 巻：96th, 2016年
J-Global ID：201602266545753865 - Hydrogels of cytoskeletal proteins: Preparation, structure, and emergent functions
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano
開始ページ：1, 終了ページ：79, 2016年01月01日
This book describes a new family of bio-polymer gels made from cytoskeletal proteins - actin, microtubule, and tropomyosin. The importance of the gel state with multi-scale hierarchical structure is emphasized to utilize emergent functions in living organisms. Detailed protocol of gel preparation, specified method of structure investigation, and dynamic studies of self-organization, self-healing, synchronized oscillating, and autonomous motility functions are introduced together with biomimetic functions of synthetic hydrogels.
英語, その他
DOI：https://doi.org/10.1007/978-3-319-27377-8
DOI ID：10.1007/978-3-319-27377-8, SCOPUS ID：84958879297 - ナノニードルアレイを用いたCreタンパク質の核内輸送
松本大亮; 齋藤恵; 加藤義雄; 川村隆三; 小林健; 岩田太; 中村史
日本化学会講演予稿集, 巻：95th, 号：3, 2015年
ISSN：0285-7626, J-Global ID：201502228386765154 - ネスチンを標的とした神経幹細胞の機械的分離技術の開発
宮崎みなみ; 清水桂太; 松本大亮; 川村隆三; 小林健; 飯嶋益巳; 黒田俊一; 岩田太; 中村史; 中村史
電気化学会大会講演要旨集(CD-ROM), 巻：82nd, 2015年
J-Global ID：201502285332423448 - 3P-206 ビメンチンを標的とした未分化ヒトiPS細胞の機械的分離(バイオプロセス,一般講演)
清水 桂太; 川村 隆三; 小林 健; 飯嶋 益巳; 黒田 俊一; 岩田 太; 中村 史
日本生物工学会大会講演要旨集, 巻：67, 開始ページ：322, 終了ページ：322, 2015年
日本生物工学会, 日本語
CiNii Articles ID：110010009621, CiNii Books ID：AN10549378 - 1P-257 未分化iPS 細胞分離に向けた免疫力学検出(バイオプロセス,一般講演)
清水 桂太; 川村 隆三; 飯嶋 益巳; 黒田 俊一; 深澤 今日子; 石原 一彦; 中村 史
日本生物工学会大会講演要旨集, 巻：66, 開始ページ：82, 終了ページ：82, 2014年
日本生物工学会, 日本語
CiNii Articles ID：110009906338, CiNii Books ID：AN10549378 - 細胞骨格トレッドミルマシン : バイオアクチュエータとしての可能性
川村 隆三; 佐野 健一; 長田 義仁
設計工学, 巻：48, 号：1, 開始ページ：11, 終了ページ：15, 2013年01月05日
日本設計工学会, 日本語
ISSN：0919-2948, CiNii Articles ID：10031146082, CiNii Books ID：AN10402165 - 2P-172 抗体修飾ナノニードルを用いた細胞の機械的分離(セル&ティッシュエンジニアリング,一般講演)
宮崎 みなみ; 清水 桂太; 川村 隆三; Sathuluri Ramachandra Rao; 小林 健; 飯嶋 益巳; 黒田 俊一; 深澤 今日子; 石原 一彦; 岩田 太; 中村 史
日本生物工学会大会講演要旨集, 巻：65, 開始ページ：147, 終了ページ：147, 2013年
日本生物工学会, 日本語
CiNii Articles ID：110009737838, CiNii Books ID：AN10549378 - 2P-171 BAM-BSA/BSA混合被膜による細胞弱接着基板の作製(セル&ティッシュエンジニアリング,一般講演)
三島 麻里; 川村 隆三; 大小瀬 求; 中村 史
日本生物工学会大会講演要旨集, 巻：65, 開始ページ：147, 終了ページ：147, 2013年
日本生物工学会, 日本語
CiNii Articles ID：110009737837, CiNii Books ID：AN10549378 - 2Bp15 ナノニードルの細胞挿入における加振の効果(バイオプロセス/センサー,計測工学/セル&ティッシュエンジニアリング,一般講演)
柳 昇桓; Silberberg Yaron; 川村 隆三; 中村 史
日本生物工学会大会講演要旨集, 巻：64, 開始ページ：31, 終了ページ：31, 2012年
日本生物工学会, 日本語
CiNii Articles ID：110009609366, CiNii Books ID：AN10549378 - 2Bp14 ナノニードルアレイの抗体修飾方法の検討(バイオプロセス/センサー,計測工学/セル&ティッシュエンジニアリング,一般講演)
下奥 万梨恵; 川村 隆三; 石原 一彦; 深沢 今日子; 中村 史
日本生物工学会大会講演要旨集, 巻：64, 開始ページ：31, 終了ページ：31, 2012年
日本生物工学会, 日本語
CiNii Articles ID：110009609365, CiNii Books ID：AN10549378 - ATP駆動型ソフトアクチュエーターの創製-配向微小管の極性評価および形成メカニズムの解明-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; きょう剣萍
高分子学会北海道支部研究発表会講演要旨集, 巻：44th, 2010年
J-Global ID：201002246797602010 - ATP駆動型アクチュエータの創製-配向微小管集合体の作製とその極性評価-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
高分子学会予稿集(CD-ROM), 巻：58, 号：2 Disk1, 2009年
J-Global ID：200902209211232097 - 配向微小管によるアクティブトランスポーターの創製
吉田桃子; 敷中一洋; 川村隆三; 田村圭輝; 角五彰; 角五彰; 古川英光; きょう剣萍
高分子学会北海道支部研究発表会講演要旨集, 巻：43rd, 2009年
J-Global ID：200902241524606665 - ATP駆動型アクチュエータの創製-配向微小管集合体の作製とその運動評価-
吉田桃子; 田村圭輝; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
高分子ゲル研究討論会講演要旨集, 巻：20th, 2009年
J-Global ID：200902252143173012 - ATP駆動型ソフトアクチュエーターの創製-配向微小管集合体の作製とその運動評価-
田村圭輝; 吉田桃子; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
繊維学会予稿集, 巻：64, 号：1, 2009年
J-Global ID：200902253094425287 - 配向微小管によるアクティブトランスポーターの創製
吉田桃子; 田村圭樹; 敷中一洋; 川村隆三; 角五彰; 角五彰; 古川英光; GONG Jian Ping
高分子学会予稿集(CD-ROM), 巻：58, 号：1 Disk1, 2009年
J-Global ID：200902254038450402 - Integration of biomolecular motors Toward an ATP fueled soft biomachine
Akira Kakugo; Kazuhiro Shikinaka; Ryuzo Kawamura; Jianping Gong
巻：151, 号：4, 開始ページ：444, 終了ページ：444, 2008年12月
英語, 研究発表ペーパー・要旨（国際会議）
DOI：https://doi.org/10.1016/j.cbpb.2008.09.062
DOI ID：10.1016/j.cbpb.2008.09.062, ISSN：1096-4959, Web of Science ID：WOS:000261672100019 - 放射光小角X線散乱を用いた配向性を有する微小管集合体の構造評価
敷中一洋; 吉田桃子; 川村隆三; 角五彰; 古川英光; GONG Jian Ping; 増永啓康; 佐々木園; 佐々木園; 増井友美; 小泉智
高分子学会予稿集(CD-ROM), 巻：57, 号：2 Disk1, 2008年
J-Global ID：200902281758612202 - 配向性を有する微小管集合体の作製とその極性評価-ATP駆動型アクティブトランスポーターを目指して-
吉田桃子; 川村隆三; 敷中一洋; 角五彰; 古川英光; GONG Jian Ping
高分子学会予稿集(CD-ROM), 巻：57, 号：1 Disk1, 2008年
J-Global ID：200902299585094908 - ポリカチオンの拡散が誘起する微小管の三次元ネットワーク形成
吉田桃子; 川村隆三; 角五彰; 古川英光; きょう剣萍; きよう剣萍; 長田義仁
高分子学会北海道支部研究発表会講演要旨集, 巻：41st, 2007年
J-Global ID：200902234616557923 - キネシンによる微小管の動的自己組織化-ATP駆動型アクチュエーターを目指して
川村隆三; 角五彰; 古川英光; GONG Jian Ping; GONG Jian Ping; 長田義仁
高分子学会予稿集(CD-ROM), 巻：56, 号：1 Disk1, 2007年
J-Global ID：200902255747172706
- Soft Actuators 2nd edition (Eds. Kinji Asaka & Hidenori Okuzaki)
Ryuzo Kawamura; Ken-Ichi Sano; Yoshihito Osada, ［分担執筆］, Chapter 40 "Employing Cytoskeletal Treadmilling in Bio-actuators"
Springer, 2019年 - Hydrogels of Cytoskeletal Proteins —Preparation, Structure, and Emergent Functions
Yoshihito Osada; Ryuzo Kawamura; Ken-Ichi Sano, ［共著］, pp 1–70
Springer International Publishing, Switzerland, 2016年 - 設計工学
川村隆三; 佐野健一; 長田義仁, ［共著］, 「細胞骨格トレッドミルマシン ―バイオアクチェータとしての可能性―」 p. 11-15
設計工学会, 2013年 - 未来材料 11月号
川村隆三; 龔 剣萍, ［共著］, 「モータータンパク質でソフトアクチュエーター ―ナノからマクロへ―」
エヌ・ティー・エス, 2008年11月 - Formation of motile assembly of microtubules driven by kinesins. Intelligent Materials (M Shahinpoor, H.-J Schneider)
Ryuzo Kawamura; Akira Kakugo; Yoshihito Osada; Jian Ping Gong, ［共著］, pp. 464–477
RSC Publishing, UK, 2007年
- ATP-fueled active network of microtubules as a dynamic environemnt for cells
Ryuzo Kawamura
2nd GLowing Polymer Symposium in KANTO, 2019年11月, ［国際会議］
英語, 口頭発表（一般） - Active network of motor proteins as artificial dynamic microenvironment for cells
Ryuzo Kawamura; Keisuke Meguriya; Tsuyoshi Yokoyama; Daiki Uehara; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
ISAGMSM(The 3rd International Symposium for Advanced Gel Materials & Soft Matters), 2019年06月, ［国際会議］
英語, 口頭発表（招待・特別） - Building up a Multi-Channel Flow System to Analyze Cellular
Ryuzo Kawamura; Tsuyoshi Yokoyama; Keisuke Meguriya; Naritaka Kobayashi; Seiichiro Nakabayashi; Hiroshi Y. Yoshikawa
GelSympo2018, 2018年08月, ［招待有り］, ［国際会議］
英語, 口頭発表（招待・特別） - キネシン・微小管の運動集積化と動的細胞環境としての応用
川村 隆三
2017年度分子モーター討論会, 2017年, ［国内会議］
日本語, シンポジウム・ワークショップパネル（指名） - キネシン・微小管を用いた運動界面構築と細胞培養基板としての応用
川村 隆三
第５回 次世代の物質科学・ナノサイエンスを探る, 2016年, ［招待有り］, ［国内会議］
日本語, シンポジウム・ワークショップパネル（指名） - 微小管でつくる水中駆動マイクロマシン
川村 隆三
第３回ＬＳＷ研究会シンポジウム, 2008年, ［招待有り］, ［国内会議］
日本語, シンポジウム・ワークショップパネル（指名）
- 物理化学Ⅲ, 埼玉大学
- 合成解析実験Ⅱ（学生実験）, 埼玉大学
- 化学基礎実験Ⅱ（学生実験）, 埼玉大学
- 化学基礎実験Ⅰ（学生実験）, 埼玉大学
- 現代の化学, 埼玉大学
- 生命工学フロンティア特論Ⅱ・Ⅴ（集中講義）, 東京農工大学
■ 共同研究・競争的資金等の研究課題
- レーザー技術による機能性分子の秩序構造形成制御
日本学術振興会, 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B)), 国際共同研究加速基金(国際共同研究強化(B)), 2019年10月 - 2024年03月
吉川 洋史; 新家 寛正; 川村 隆三, 埼玉大学
配分額（総額）：18460000, 配分額（直接経費）：14200000, 配分額（間接経費）：4260000
本国際共同研究の目的は、台湾および日本で独自開発された２種類のレーザー結晶化技術を基盤とした秩序構造形成法を開発し、従来の性能や機能を超える革新的材料を創成することにある。
まず２０１９年度は主に「レーザートラッピングとレーザーアブレーションを組み合わせた新しい結晶化制御法の開発」に取り組んだ。ここでは、海外共同研究者と研究代表者それぞれが開発してきた原理の異なる２種類のレーザー結晶化技術を組み合わせた新規手法を開発した。本手法では、光源として高繰り返しのフェムト秒レーザーを用いて対物レンズで溶液中に集光する。すると、まず最初にレーザートラッピングの効果により集光点での濃度上昇が起こり、その後に溶液のレーザーアブレーションによりキャビテーションバブルが発生する。我々は、これらの現象が相乗的に作用し、高効率で結晶化を誘起することが可能であることを見出した。また興味深いことに、結晶化後もレーザーを照射し続けると、核発生時とは異なる構造を有する結晶が生じることを発見した。条件を最適化すると、L-フェニルアラニンを用いたケースでは、準安定形から安定形だけでなく、安定形から準安定形の結晶を作製することも可能であった。このような両方向の多形制御は非常にユニークであり、レーザーを用いることで実現される新しい結晶化制御アプローチとして期待している。本成果は、国際共同研究者との共著論文としてJapanese Journal of Physical Chemistry誌に発表している。また上記の研究以外にも、レーザートラッピングによる結晶化ダイナミクスの解明（The Journal of Physical Chemistry Letters誌に掲載）や、様々な有機物・無機物の機能性材料の作製に関する研究も展開した。
課題番号：19KK0128 - モータータンパク質駆動型揺らぎプロセッサの創成
JKA, 研究補助（複数年）, 2021年04月 - 2023年03月
川村隆三, 埼玉大学, 研究代表者 - 光物理的アプローチによる細胞骨格形成の自在制御技術の開発
日本学術振興会, 科学研究費助成事業 挑戦的研究(萌芽), 挑戦的研究(萌芽), 2020年07月 - 2023年03月
吉川 洋史; 川村 隆三; 杉山 輝樹, 埼玉大学
配分額（総額）：6370000, 配分額（直接経費）：4900000, 配分額（間接経費）：1470000
本年度は、前年度までに見出したレーザーによる細胞骨格形成現象について、そのメカニズムに関する研究を実施した。そのために特に、集光レーザービームに基づく熱および電場の影響について調べた。まず熱作用の検証については、従来の水溶媒に加えて、重水を使用する実験系を立ち上げた。重水は、レーザー波長（1064 nm）における吸収係数が水より大幅に小さいため、レーザーによって発生する熱が細胞骨格形成に与える影響を調べることができる。本研究では、水と重水の混合比を変えながら細胞骨格形成現象を系統的に調べた。その結果、重水の比率が大きくなるほど、形成する細胞骨格のサイズが小さくなり、形成に要する時間も長くなることがわかった。また電場作用については、レーザーの偏光（例：直線偏光、円偏光）が細胞骨格形成現象に与える影響について検証した。その結果、細胞骨格形成が偏光方向に依存するという興味深い結果を見出した。以上の結果は、レーザーによる細胞骨格形成現象が、熱および電場の両方の作用により生じていることを示唆している。以上の成果は、コロイドおよび界面化学討論会やCSJ化学フェスタなどで学会発表した。また他にもレーザーを照射する界面状態が細胞骨格形成に与える影響についても検証し、興味深い知見を得ている。また細胞骨格形成に関する研究と並行して、本研究の基盤技術であるレーザー操作法の高度化に関する研究も実施し、学会などで成果を発表した。
課題番号：20K21117 - 超高速分子ディスプレイを用いた分子知能機械の研究
日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2019年04月 - 2023年03月
星野 隆行; 川村 隆三, 弘前大学
配分額（総額）：17420000, 配分額（直接経費）：13400000, 配分額（間接経費）：4020000
本研究は，電子線の電気的な性質を用い，誘電体膜であるSiN薄膜の裏面から電子線を照射して，仮想的な電極パターン（バーチャル電極）を誘導する技術を基盤としている．このバーチャル電極を動的に走査することにより，SiN薄膜上にある分子機能を操作することが研究の目的である．
本年度においては，バーチャル電極を動的に走査するシステムを構築し，電子線を高速に走査することにより2次元のバーチャル電極パターンを描画し，さらにそのパターンを自在に平行移動・回転・変形できることを示した．この動的に制御できるバーチャル電極を用いて，蛍光標識したdsDNA分子に対して適用したところ，dose量が同じであっても走査速度に依存して応答が変わることが分かった．バーチャル電極が投影されつディスプレイ面であるSiN薄膜の誘電特性や，固液界面に生じる電気二重層がハイパスフィルターの周波数特性を有していおり，バーチャル電極に誘電的な周波数特性を付与しているものと考えられる．これらの結果は，バーチャル電極パターンをより高速な走査により投影することにより，より広い面積をより高い効果を維持して分子機能操作を実行できることを示唆している．走査型プローブによる分子操作においては，時分割で2次元の場を提示することは，エネルギー密度の低下を招くことから大面積のパターン呈示は困難であると考えられるが，本研究のバーチャル電極ではハイパス特性によりエネルギー密度の低下が相殺されることが期待できる．
また，これまで取り組んでいた人工脂質膜の形状をバーチャル電極により制御する研究が論文化ができ，Inside Back Cover に掲載される成果をあげられた．
課題番号：19H02100 - キネシン駆動型運動界面を利用した細胞の動的力学環境構築
文部科学省, 卓越研究員事業, 2017年10月 - 2022年09月
川村 隆三, 研究代表者
競争的資金 - 運動界面によるがん細胞の転移性評価指標の導出
日本学術振興会, 科学研究費助成事業 基盤研究(C), 基盤研究(C), 2019年04月 - 2022年03月
川村 隆三, 埼玉大学
配分額（総額）：4290000, 配分額（直接経費）：3300000, 配分額（間接経費）：990000
本研究の目的は、がん細胞の潜在的な転移性を示す新しい細胞計測の指標を得ることである。研究代表者は、キネシン・微小管のモータータンパク質を用いて『運動界面』を作製し、細胞の動的力学環境へ応用する研究に取り組んでいる。転移性の高いがん細胞（マウスメラノーマ）が運動界面で揺られながら接着すると、細胞の内部に特異なアクチン凝集を形成することを発見していた。アクチン繊維を架橋して突起形成を促すタンパク質の阻害剤を投与すると、このアクチン凝集が抑制されることから、アクチン凝集はがん細胞の浸潤突起形成能を示す可能性を考えた。本研究では、このアクチン凝集形成をがん細胞の潜在的な転移性を示す指標になる可能性を調べる。このため、運動界面の設計を最適化し、揺らぎ力学刺激がアクチン凝集形成に作用するメカニズムを解明する。また、運動界面を既存の細胞挙動評価法と組み合わせて、がん細胞の転移に伴う力学的状況をより生体内に近づけて、がん細胞の潜在的な転移性を最大限に引き出して計測することを目指している。
令和元年度は、運動界面に接着する細胞の形状を評価するシステムの自動化を進め、従来よりも多検体を安定的に定量評価する技術的な開発を進めた。改良したシステムを用い、運動界面にがん細胞が接着する過程での細胞構造変化を経過時間ごとに解析することに成功している。今後は、システムを簡便に操作するための改良を加えて、運動界面の設計パラメーター変化の影響や、他の細胞株を用いた比較などのデータを蓄積し、がん細胞株の潜在的な転移性を診断する指標になるかどうかを検証していく。
主要な計画ではなかったが、運動界面の出力を直接的に力学計測する技術を実現した。これにより、運動界面の設計パラメーターに変化加えた場合などに出力特性の違いを評価できるようになった。今後は、この手法も活用した運動界面の開発が見込める。
課題番号：19K12775 - 運動界面を利用した転移性がん細胞の変形応答評価系の開発
日揮・実吉奨学会, 研究助成, 2018年10月 - 2020年03月
川村 隆三, 研究代表者
競争的資金 - 微小管の秩序的ネットワーク化による運動界面構築
日本学術振興会, 科学研究費助成事業 若手研究(B), 若手研究(B), 2015年04月 - 2017年03月
川村 隆三; 中林 誠一郎; 吉川 洋史; 小林 成貴; 菅沼 雅美, 埼玉大学
配分額（総額）：4290000, 配分額（直接経費）：3300000, 配分額（間接経費）：990000
架橋した微小管のネットワークとキネシンで構築する「運動界面」について、協同性の高い運動を発現する原理の探求と、細胞の動的力学環境としての応用に取り組んだ。モーター分子であるキネシンを基板に２次元パターン修飾することで、微小管の進行方向の制御と運動界面領域のパターン作成を実現した。接着した細胞に隣接して運動界面を配置して駆動し、力学刺激を付与することにも成功している。運動界面上に一度剥離した細胞を播種すると、再接着後の細胞形態に力学刺激への応答が観察される現象を発見した。
課題番号：15K17451 - ポリマー材料中におけるカーボンドットのメカノクロミズム機能探索
マツダ財団, 第３３回マツダ研究助成, 2017年
川村 隆三, 研究代表者
競争的資金 - がん細胞の硬化に着目した新規転移抑制物質の探索と分子構造の解明（分担）
武田科学振興財団, 特定研究助成, 2016年
菅沼 雅美
競争的資金