Performance information

• Emergence of the sex difference in calbindin D-28K neurons and the role of calbindin D-28K in clustering neurons of the preoptic area in mice.　　　　　　　　　　　　　　　
  Ryoma Koiso; Chihiro Kushida; Moeko Kanaya; Shinji Tsukahara
  Cell and tissue research, May 2025, [Reviewed], ［International magazine］
  Neurons expressing Calb1 that encodes calbindin D-28K (Calb), a calcium-binding protein, are significant components of the sexually dimorphic nucleus (SDN) of the preoptic area in mice. Calb is therefore used as a marker to study the SDN. The number of Calb neurons in the SDN is greater in males than in females, and this sex difference emerges before puberty. However, the timing of emergence of this difference and the role of Calb1 in the SDN remain unclear. In this study, we investigated when the sex difference in Calb neurons appears and whether Calb1 is required for organizing the SDN. Profiling the temporal changes in Calb neurons revealed that these neurons dramatically increased in the SDN during postnatal days 15 to 20 in males but not in females, resulting in a significant difference between sexes. However, the Calb1 mRNA in the SDN remained unchanged during the late postnatal period and did not differ between sexes. Calb1 knockdown (KD) was performed by injecting an adeno-associated virus vector into the preoptic area of neonatal mice. The analysis of postnatal Calb1-KD mice revealed that Calb1 KD reduced not only Calb1 expression but also the number of neurons comprising the SDN. These findings suggest that compared with the female SDN, more Calb neurons migrate to and are incorporated into the male SDN, thereby yielding the sex difference in the number of Calb neurons at the late postnatal period, and that Calb1 plays a significant role in clustering neurons in the SDN.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00441-025-03981-3
  DOI ID：10.1007/s00441-025-03981-3, PubMed ID：40397161
• Distinct nociception processing in the dysgranular and barrel regions of the mouse somatosensory cortex.　　　　　　　　　　　　　　　
  Hironobu Osaki; Moeko Kanaya; Yoshifumi Ueta; Mariko Miyata
  Nature communications, Volume：13, Number：1, First page：3622, Last page：3622, Jun. 2022, [Reviewed], ［International magazine］
  Nociception, a somatic discriminative aspect of pain, is, like touch, represented in the primary somatosensory cortex (S1), but the separation and interaction of the two modalities within S1 remain unclear. Here, we show spatially distinct tactile and nociceptive processing in the granular barrel field (BF) and adjacent dysgranular region (Dys) in mouse S1. Simultaneous recordings of the multiunit activity across subregions revealed that Dys neurons are more responsive to noxious input, whereas BF neurons prefer tactile input. At the single neuron level, nociceptive information is represented separately from the tactile information in Dys layer 2/3. In contrast, both modalities seem to converge on individual layer 5 neurons of each region, but to a different extent. Overall, these findings show layer-specific processing of nociceptive and tactile information between Dys and BF. We further demonstrated that Dys activity, but not BF activity, is critically involved in pain-like behavior. These findings provide new insights into the role of pain processing in S1.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-022-31272-w
  DOI ID：10.1038/s41467-022-31272-w, PubMed ID：35768422
• Optimization of immunohistochemical detection of rat ESR2 proteins with well-validated monoclonal antibody PPZ0506.　　　　　　　　　　　　　　　
  Yujiro Hattori; Hirotaka Ishii; Shimpei Higo; Mai Otsuka; Moeko Kanaya; Keisuke Matsumoto; Mina Ozawa; Hitoshi Ozawa
  Molecular and cellular endocrinology, Volume：523, First page：111145, Last page：111145, Mar. 2021, [Reviewed], ［International magazine］
  Although there are few well-validated antibodies against ESR2 proteins, a recent validation assessment identified a specific monoclonal antibody against human ESR2 proteins (PPZ0506). Furthermore, our previous study confirmed its cross-reactivity and specificity against rodent ESR2 proteins, enabling the determination of true ESR2 distribution profiles in rodents. Therefore, we aimed to determine optimal conditions for ESR2 detection by PPZ0506 immunostaining and analyze ESR2 distribution in rats. We evaluated several staining conditions using paraffin-embedded and frozen ovary sections. Immunohistochemical staining with PPZ0506 antibody required strong antigen retrieval and appropriate antibody dilution. Subsequent immunohistochemical analysis in multiple tissues under optimized conditions revealed that rat ESR2 proteins are expressed in a more localized manner than previously assumed. Our results suggest that previous immunohistochemical studies using inadequately validated antibodies against ESR2 proteins overestimated their distribution profiles. We expect that optimized immunohistochemical detection with PPZ0506 antibody can help researchers solve several conflicting problems in ESR2 research.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.mce.2020.111145
  DOI ID：10.1016/j.mce.2020.111145, PubMed ID：33400952
• Expression analysis of neuropeptide FF receptors on neuroendocrine-related neurons in the rat brain using highly sensitive in situ hybridization.　　　　　　　　　　　　　　　
  Shimpei Higo; Moeko Kanaya; Hitoshi Ozawa
  Histochemistry and cell biology, Jan. 2021, [Reviewed], ［International magazine］
  RF-amide peptides, a family of peptides characterized by a common carboxy-terminal Arg-Phe-NH2 motif, play various physiological roles in the brain including the modulation of neuroendocrine signaling. Neuropeptide FF (NPFF) receptors exhibit a high affinity for all RF-amide peptides, which suggests that the neurons expressing these NPFF receptors may have multiple functions in the brain. However, the distribution of the neurons expressing NPFF receptors in the rat brain remains poorly understood. This study aimed to determine the detailed histological distribution of mRNA that encodes the neuropeptide FF receptors (Npffr1 and Npffr2) in the rat brain using in situ hybridization. Neurons with strong Npffr1 expression were observed in the lateral septal nucleus and several hypothalamic areas related to neuroendocrine functions, including the paraventricular nucleus (PVN) and arcuate nucleus, whereas Npffr2-expressing neurons were observed mainly in brain regions involved in somatosensory pathways, such as several subnuclei of the thalamus. Npffr1 expression was observed in 70% of corticotropin-releasing hormone neurons, but in only a small population of oxytocin and vasopressin neurons in the PVN. Npffr1 expression was also observed in the dopaminergic neurons in the periventricular nucleus and the dorsal arcuate nucleus, and in the kisspeptin neurons in the anteroventral periventricular nucleus. These results suggest that NPFFR1-mediated signaling may be involved in neuroendocrine functions, such as in reproduction and stress response. In conjunction with a detailed histological map of NPFFRs, this study provides useful data for future neuroendocrine research.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00418-020-01956-9
  DOI ID：10.1007/s00418-020-01956-9, PubMed ID：33398437
• Neurochemical Characterization of Neurons Expressing Estrogen Receptor β in the Hypothalamic Nuclei of Rats Using in Situ Hybridization and Immunofluorescence.　　　　　　　　　　　　　　　
  Moeko Kanaya; Shimpei Higo; Hitoshi Ozawa
  International journal of molecular sciences, Volume：21, Number：1, Dec. 2019, [Reviewed], [Lead], ［International magazine］
  Estrogens play an essential role in multiple physiological functions in the brain, including reproductive neuroendocrine, learning and memory, and anxiety-related behaviors. To determine these estrogen functions, many studies have tried to characterize neurons expressing estrogen receptors known as ERα and ERβ. However, the characteristics of ERβ-expressing neurons in the rat brain still remain poorly understood compared to that of ERα-expressing neurons. The main aim of this study is to determine the neurochemical characteristics of ERβ-expressing neurons in the rat hypothalamus using RNAscope in situ hybridization (ISH) combined with immunofluorescence. Strong Esr2 signals were observed especially in the anteroventral periventricular nucleus (AVPV), bed nucleus of stria terminalis, hypothalamic paraventricular nucleus (PVN), supraoptic nucleus, and medial amygdala, as previously reported. RNAscope ISH with immunofluorescence revealed that more than half of kisspeptin neurons in female AVPV expressed Esr2, whereas few kisspeptin neurons were found to co-express Esr2 in the arcuate nucleus. In the PVN, we observed a high ratio of Esr2 co-expression in arginine-vasopressin neurons and a low ratio in oxytocin and corticotropin-releasing factor neurons. The detailed neurochemical characteristics of ERβ-expressing neurons identified in the current study can be very essential to understand the estrogen signaling via ERβ.
  English
  DOI：https://doi.org/10.3390/ijms21010115
  DOI ID：10.3390/ijms21010115, PubMed ID：31877966, PubMed Central ID：PMC6981915
• Applicability of Anti-Human Estrogen Receptor β Antibody PPZ0506 for the Immunodetection of Rodent Estrogen Receptor β Proteins.　　　　　　　　　　　　　　　
  Hirotaka Ishii; Mai Otsuka; Moeko Kanaya; Shimpei Higo; Yujiro Hattori; Hitoshi Ozawa
  International journal of molecular sciences, Volume：20, Number：24, Dec. 2019, [Reviewed], ［International magazine］
  Several lines of controversial evidence concerning estrogen receptor β (ERβ) remain to be solved because of the unavailability of specific antibodies against ERβ. The recent validation analysis identified a monoclonal antibody (PPZ0506) with sufficient specificity against human ERβ. However, the specificity and cross-reactivity of PPZ0506 antibody against ERβ proteins from laboratory animals have not been confirmed. In the present study, we aimed to validate the applicability of PPZ0506 to rodent studies. The antibody exhibited specific cross-reactivity against mouse and rat ERβ proteins in immunoblot and immunocytochemical experiments using transfected cells. In immunohistochemistry for rat tissue sections, PPZ0506 showed immunoreactive signals in the ovary, prostate, and brain. These immunohistochemical profiles of rat ERβ proteins in rat tissues accord well with its mRNA expression patterns. Although the antibody was reported to show the moderate signals in human testis, no immunoreactive signals were observed in rat testis. Subsequent RT-PCR analysis revealed that this species difference in ERβ expression resulted from different expression profiles related to the alternative promoter usage between humans and rats. In conclusion, we confirmed applicability of PPZ0506 for rodent ERβ studies, and our results provide a fundamental basis for further examination of ERβ functions.
  English, Scientific journal
  DOI：https://doi.org/10.3390/ijms20246312
  DOI ID：10.3390/ijms20246312, PubMed ID：31847265, PubMed Central ID：PMC6941125
• Neonatal septal lesions prevent behavioral defeminization caused by neonatal treatment with estradiol in female rats.　　　　　　　　　　　　　　　
  Kanaya M; Tsukahara S; Yamanouchi K
  Neuroscience letters, Volume：694, First page：80, Last page：85, Feb. 2019, [Reviewed], [Lead, Corresponding]
  DOI：https://doi.org/10.1016/j.neulet.2018.11.032
  DOI ID：10.1016/j.neulet.2018.11.032, ISSN：0304-3940, PubMed ID：30472357
• Temporal expression patterns of genes related to sex steroid action in sexually dimorphic nuclei during puberty　　　　　　　　　　　　　　　
  Moeko Kanaya; Masahiro Morishita; Shinji Tsukahara
  Frontiers in Endocrinology, Volume：9, Number：MAY, First page：213, May 2018, [Reviewed], [Lead]
  Frontiers Media S.A., English, Scientific journal
  DOI：https://doi.org/10.3389/fendo.2018.00213
  DOI ID：10.3389/fendo.2018.00213, ISSN：1664-2392, PubMed ID：29770127, SCOPUS ID：85046672261
• Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Kinuyo Iwata; Hitoshi Ozawa
  BIOLOGY OF REPRODUCTION, Volume：97, Number：5, First page：709, Last page：718, Nov. 2017, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1093/biolre/iox131
  DOI ID：10.1093/biolre/iox131, ISSN：0006-3363, eISSN：1529-7268, PubMed ID：29069289, Web of Science ID：WOS:000418963100006
• Regional Difference in Sex Steroid Action on Formation of Morphological Sex Differences in the Anteroventral Periventricular Nucleus and Principal Nucleus of the Bed Nucleus of the Stria Terminalis　　　　　　　　　　　　　　　
  Moeko Kanaya; Mumeko C. Tsuda; Shoko Sagoshi; Kazuyo Nagata; Chihiro Morimoto; Chaw Kyi Tha Thu; Katsumi Toda; Shigeaki Kato; Sonoko Ogawa; Shinji Tsukahara
  PLOS ONE, Volume：9, Number：11, First page：e112616, Nov. 2014, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0112616
  DOI ID：10.1371/journal.pone.0112616, ISSN：1932-6203, PubMed ID：25398007, Web of Science ID：WOS:000345558500066
• Neuroanatorny and sex differences of the lordosis-inhibiting system in the lateral septum　　　　　　　　　　　　　　　
  Shinji Tsukahara; Moeko Kanaya; Korehito Yamanouchi
  FRONTIERS IN NEUROSCIENCE, Volume：8, First page：299, Sep. 2014, [Reviewed]
  English
  DOI：https://doi.org/10.3389/fnins.2014.00299
  DOI ID：10.3389/fnins.2014.00299, ISSN：1662-453X, Web of Science ID：WOS:000346527100001
• Involvement of hemeoxygenase-1 in di(2-ethylhexyl) phthalate (DEHP)-induced apoptosis of Neuro-2a cells　　　　　　　　　　　　　　　
  Kyaw Htet Aung; Tin-Tin Win-Shwe; Moeko Kanaya; Hirohisa Takano; Shinji Tsukahara
  JOURNAL OF TOXICOLOGICAL SCIENCES, Volume：39, Number：2, First page：217, Last page：229, Apr. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.2131/jts.39.217
  DOI ID：10.2131/jts.39.217, ISSN：0388-1350, eISSN：1880-3989, PubMed ID：24646702, Web of Science ID：WOS:000334013300005
• Defeminization of brain functions by a single injection of estrogen receptor α or β agonist in neonatal female rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Korehito Yamanouchi
  Neuroendocrinology, Volume：95, Number：4, First page：297, Last page：304, Jun. 2012, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1159/000332128
  DOI ID：10.1159/000332128, ISSN：0028-3835, PubMed ID：22327340, SCOPUS ID：84862775880

• 【形態学の逆襲:形態学教室の扉をたたいてみてください】In situ hybridization法の高感度化手法とその神経科学未解明領域への応用　　　　　　　　　　　　　　　
  肥後 心平; 金谷 萌子; 水野 友喜; 小澤 一史; 坂本 篤裕; 石井 寛高
  Volume：19, Number：2, First page：84, Last page：89, Apr. 2023
  Japanese
  ISSN：1349-8975, eISSN：1880-2877, 医中誌Web ID：X526360003

• 慢性疼痛モデルマウスの免疫細胞遺伝子発現に対する性ホルモンの影響　　　　　　　　　　　　　　　
  金谷萌子; 髙橋友紀乃
  Oct. 2024
• 痛み行動の性差に対する新生期の性ステロイド処置の影響　　　　　　　　　　　　　　　
  金谷萌子; 宮田麻理子
  Jul. 2021, [Domestic conference]
  Japanese, Poster presentation, Japan
• 抗ヒトERβ特異的モノクローナル抗体(PPZ0506)を用いたラット組織に対する免疫組 織化学的検出方法の最適化　　　　　　　　　　　　　　　
  服裕次郎; 石井寛高; 肥後心平; 大塚真衣; 金谷萌子; 松本恵介; 呉立洋; 小澤実那; 小澤一史
  Dec. 2020, [Domestic conference]
  Japanese, Poster presentation, Japan
• 齧歯類における抗ヒトERβ抗体(PPZ0506)の交差性・特異性検証とERβ発現分布解析　　　　　　　　　　　　　　　
  大塚真衣; 石井寛高; 金谷萌子; 肥後心平; 小澤一史
  Mar. 2020, [Domestic conference]
  Japanese, Poster presentation, Japan
• 抗ヒトERβ抗体（PPZ0506）のマウス・ラットERβに対する交差性・特異性の検証とERβ発現プロファイルの同定　　　　　　　　　　　　　　　
  大塚真衣; 石井寛高; 金谷萌子; 肥後心平; 小澤一史
  Sep. 2019, [Domestic conference]
  Japanese, Poster presentation, Japan
• 女性研究者セッション～組織細胞化学研究の魅力を語る～生殖機能制御を中心としたこれまでの研究について　　　　　　　　　　　　　　　
  岩田衣世; 金谷萌子; 國村有弓; 村川裕子; 小澤一史
  Sep. 2019, [Domestic conference]
  Japanese, Nominated symposium, Japan
• ラットの視床下部神経核におけるエストロゲン受容体βの神経化学特性の同定　　　　　　　　　　　　　　　
  金谷萌子; 肥後心平; 小澤一史
  Mar. 2019, [Domestic conference]
  Japanese, Poster presentation, Japan
• Neurochemical characterization of neurons expressing estrogen receptor-beta in the hypothalamus of rats using RNAscope in situ hybridization and immunofluoresecence　　　　　　　　　　　　　　　
  Moeko Kanaya; Shimpei Higo; Hitoshi Ozawa
  10th Internationalmeeting Steroids and Nervous System, Feb. 2019, [International conference]
  English, Poster presentation, Torino, Italy, Italy
• 新たなin situ hybridization法によるエストロゲン受容体βの発現解析　　　　　　　　　　　　　　　
  金谷萌子; 肥後心平; 小澤一史
  Sep. 2018, [Domestic conference]
  Japanese, Oral presentation, Japan
• 性的二型核の性差形成における性ステロイドの作用機構　　　　　　　　　　　　　　　
  金谷萌子
  Sep. 2018, [Invited], [Domestic conference]
  Japanese, Invited oral presentation, Japan
• エストロゲンの濃度変化における雌ラット弓状核dynorphinの発現変動　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  Mar. 2017, [Domestic conference]
  Japanese, Poster presentation, Japan
• 雌ラット弓状核のdynorphin発現に対するエストロゲンのネガティブフィードバック制御　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  Oct. 2016, [Domestic conference]
  Japanese, Oral presentation, Japan
• エストロゲンの濃度変化による弓状核dynorphinの発現変動　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  Sep. 2016, [Domestic conference]
  Japanese, Oral presentation, Japan
• 性的二型核の性差形成における周生期と思春期の性ステロイド作用　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; Chaw Kyi Tha Thu; 戸田勝巳; 加藤茂明; 小川園子; 塚原伸治
  Sep. 2015, [Domestic conference]
  Japanese, Oral presentation, Japan
• 性行動と脳構造の性分化における性ステロイドホルモンの役割　　　　　　　　　　　　　　　
  金谷萌子
  Dec. 2014, [Invited], [Domestic conference]
  Japanese, Invited oral presentation, Japan
• 前腹側脳室周囲核と分界条床核主核の性差形成に対する性ステロイド作用の相違性と類似性　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; Chaw Kyi Tha Thu; 戸田勝巳; 加藤茂明; 小川園子; 塚原伸治
  Sep. 2014, [Domestic conference]
  Japanese, Poster presentation, Japan
• Regional differences in sexual steroid action on formation of sexually dimorphic nuclei in mice　　　　　　　　　　　　　　　
  Moeko Kanaya; Mumeko C. Tsuda; Shoko Sagoshi; Kazuyo Nagata; Chihiro Morimoto; Chaw Kyi Tha Thu; Katsumi Toda; Shigeaki Kato; Sonoko Ogawa; Shinji Tsukahara
  The 8th International Congress of Neuroendocrinology 2014, Aug. 2014, [International conference]
  English, Poster presentation, Sydney, Australia, Australia
• マウスの性的二型核形成に関与する性ステロイド作用の部位差　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; 栗原良平; 加藤茂明; 小川園子; 塚原伸治
  Sep. 2013, [Domestic conference]
  Japanese, Oral presentation, Japan
• Involvement of the septum on neonatal estrogen-induced behavioral defeminization in rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Shinji Tsukahara; Korehito Yamanouchi
  Neuroscience 2012, Oct. 2012, [International conference]
  English, Poster presentation, New Orleans, USA, United States
• 雌ラット新生期中隔破壊は新生期エストロゲン投与による行動の脱雌性化を阻止するが無排卵症候群は阻止しない　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  Sep. 2012, [Domestic conference]
  Japanese, Poster presentation, Japan
• 雄ラットのロードーシス発現に対する新生期中隔破壊効果　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  Sep. 2012, [Domestic conference]
  Japanese, Oral presentation, Japan
• 性行動の脱雌性化に対する新生期中隔破壊の効果　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  Aug. 2012, [Domestic conference]
  Japanese, Oral presentation, Japan
• 雌ラットの脳機能の雄性化に対するエストロゲンα, β受容体作動剤新生期投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  Nov. 2011, [Domestic conference]
  Japanese, Oral presentation, Japan
• 雌ラット視床下部ERα免疫陽性細胞数へのERα, β作動剤, 性ステロイド投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  Sep. 2011, [Domestic conference]
  Japanese, Poster presentation, Japan
• Effects of ERα and β agonists, estrogen and androgen on number of ERα-immunoreactive cells in the hypothalamus of female rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Ayako Ogata; Korehito Yamanouchi
  8th IBRO World Congress of Neuroscience, Jul. 2011, [International conference]
  English, Poster presentation, Florence, Italy, Italy
• 雌ラットの性周期とロードーシスに対するエストロゲンα, β受容体作動剤新生期投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  Jun. 2011, [Domestic conference]
  Japanese, Oral presentation, Japan
• 雌ラットにおける性周期とロードーシスに対するエストロゲンα, β受容体作動剤投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  Sep. 2010, [Domestic conference]
  Japanese, Poster presentation, Japan
• 雌ラット発情周期とロードーシスに対するエストロゲン受容体α, β作動剤新生期投与　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  Sep. 2010, [Domestic conference]
  Japanese, Oral presentation, Japan
• Defeminizing and masculinizing effects of single injection with estrogen receptor α or β agonist in neonatal female rat　　　　　　　　　　　　　　　
  Moeko Kanaya; Korehito Yamanouchi
  The 7th International Congress of Neuroendocrinology, Jul. 2010, [International conference]
  English, Poster presentation, Rouen, France, France

• JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
• THE JAPAN NEUROSCIENCE SOCIETY
• JAPAN NEUROENDOCRINE SOCIETY

• 痛みの性差を引き起こす神経回路に関する研究　　　　　　　　　　　　　　　
  Jul. 2024 - Jun. 2025
  Principal investigator
• 痛みの性差をもたらす痛覚の神経回路メカニズムの解明　　　　　　　　　　　　　　　
  Apr. 2021 - Mar. 2024
  Grant amount(Total)：4680000, Direct funding：3600000, Indirect funding：1080000
  Grant number：21K15345
• Science in gender difference of brain functions　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), 30 Jul. 2020 - 31 Mar. 2023
  Tokyo Women's Medical University
  Grant amount(Total)：6500000, Direct funding：5000000, Indirect funding：1500000
  Grant number：20K21508
• Molecular and morphofunctional study on the novel regulation system for reproduction based on the input of various enviromental information　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2018 - 31 Mar. 2022
  Ozawa Hitoshi, Nippon Medical School
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  We have been studying the function and morphology of kisspeptin, a new "main player" in the reproductive regulatory system, and its containing neurons, using histochemical and molecular biological techniques. The results include the relationship between energy metabolism and the reproductive regulatory axis, subtypes of sex hormones and their functions, distribution of kisspeptin-related peptides in the brain and their functions, effects of the maternal-fetal environment on postnatal development based on the Dohad theory, changes in reproductive regulatory mechanisms in animal models of diabetes mellitus, and the effects of kisspeptin on kisspeptin neurons. We have achieved a wide range of research accomplishments.
  Grant number：18K06860
• 発達期の性ステロイドホルモンによって規定される脳機能の性差に関する研究　　　　　　　　　　　　　　　
  Apr. 2019 - Mar. 2022
  Grant amount(Total)：4030000, Direct funding：3100000, Indirect funding：930000
  Grant number：19J00621
• Effect of genetic background on the brain regions associated with depression in postpartum depression models　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Apr. 2018 - Mar. 2021
  Kanaya Moeko
  Grant amount(Total)：3250000, Direct funding：2500000, Indirect funding：750000
  Grant number：18K16818