業績情報

• Distinct nociception processing in the dysgranular and barrel regions of the mouse somatosensory cortex.　　　　　　　　　　　　　　　
  Hironobu Osaki; Moeko Kanaya; Yoshifumi Ueta; Mariko Miyata
  Nature communications, 巻：13, 号：1, 開始ページ：3622, 終了ページ：3622, 2022年06月, ［査読有り］, ［国際誌］
  Nociception, a somatic discriminative aspect of pain, is, like touch, represented in the primary somatosensory cortex (S1), but the separation and interaction of the two modalities within S1 remain unclear. Here, we show spatially distinct tactile and nociceptive processing in the granular barrel field (BF) and adjacent dysgranular region (Dys) in mouse S1. Simultaneous recordings of the multiunit activity across subregions revealed that Dys neurons are more responsive to noxious input, whereas BF neurons prefer tactile input. At the single neuron level, nociceptive information is represented separately from the tactile information in Dys layer 2/3. In contrast, both modalities seem to converge on individual layer 5 neurons of each region, but to a different extent. Overall, these findings show layer-specific processing of nociceptive and tactile information between Dys and BF. We further demonstrated that Dys activity, but not BF activity, is critically involved in pain-like behavior. These findings provide new insights into the role of pain processing in S1.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1038/s41467-022-31272-w
  DOI ID：10.1038/s41467-022-31272-w, PubMed ID：35768422
• Optimization of immunohistochemical detection of rat ESR2 proteins with well-validated monoclonal antibody PPZ0506.　　　　　　　　　　　　　　　
  Yujiro Hattori; Hirotaka Ishii; Shimpei Higo; Mai Otsuka; Moeko Kanaya; Keisuke Matsumoto; Mina Ozawa; Hitoshi Ozawa
  Molecular and cellular endocrinology, 巻：523, 開始ページ：111145, 終了ページ：111145, 2021年03月, ［査読有り］, ［国際誌］
  Although there are few well-validated antibodies against ESR2 proteins, a recent validation assessment identified a specific monoclonal antibody against human ESR2 proteins (PPZ0506). Furthermore, our previous study confirmed its cross-reactivity and specificity against rodent ESR2 proteins, enabling the determination of true ESR2 distribution profiles in rodents. Therefore, we aimed to determine optimal conditions for ESR2 detection by PPZ0506 immunostaining and analyze ESR2 distribution in rats. We evaluated several staining conditions using paraffin-embedded and frozen ovary sections. Immunohistochemical staining with PPZ0506 antibody required strong antigen retrieval and appropriate antibody dilution. Subsequent immunohistochemical analysis in multiple tissues under optimized conditions revealed that rat ESR2 proteins are expressed in a more localized manner than previously assumed. Our results suggest that previous immunohistochemical studies using inadequately validated antibodies against ESR2 proteins overestimated their distribution profiles. We expect that optimized immunohistochemical detection with PPZ0506 antibody can help researchers solve several conflicting problems in ESR2 research.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.mce.2020.111145
  DOI ID：10.1016/j.mce.2020.111145, PubMed ID：33400952
• Expression analysis of neuropeptide FF receptors on neuroendocrine-related neurons in the rat brain using highly sensitive in situ hybridization.　　　　　　　　　　　　　　　
  Shimpei Higo; Moeko Kanaya; Hitoshi Ozawa
  Histochemistry and cell biology, 2021年01月, ［査読有り］, ［国際誌］
  RF-amide peptides, a family of peptides characterized by a common carboxy-terminal Arg-Phe-NH2 motif, play various physiological roles in the brain including the modulation of neuroendocrine signaling. Neuropeptide FF (NPFF) receptors exhibit a high affinity for all RF-amide peptides, which suggests that the neurons expressing these NPFF receptors may have multiple functions in the brain. However, the distribution of the neurons expressing NPFF receptors in the rat brain remains poorly understood. This study aimed to determine the detailed histological distribution of mRNA that encodes the neuropeptide FF receptors (Npffr1 and Npffr2) in the rat brain using in situ hybridization. Neurons with strong Npffr1 expression were observed in the lateral septal nucleus and several hypothalamic areas related to neuroendocrine functions, including the paraventricular nucleus (PVN) and arcuate nucleus, whereas Npffr2-expressing neurons were observed mainly in brain regions involved in somatosensory pathways, such as several subnuclei of the thalamus. Npffr1 expression was observed in 70% of corticotropin-releasing hormone neurons, but in only a small population of oxytocin and vasopressin neurons in the PVN. Npffr1 expression was also observed in the dopaminergic neurons in the periventricular nucleus and the dorsal arcuate nucleus, and in the kisspeptin neurons in the anteroventral periventricular nucleus. These results suggest that NPFFR1-mediated signaling may be involved in neuroendocrine functions, such as in reproduction and stress response. In conjunction with a detailed histological map of NPFFRs, this study provides useful data for future neuroendocrine research.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s00418-020-01956-9
  DOI ID：10.1007/s00418-020-01956-9, PubMed ID：33398437
• Neurochemical Characterization of Neurons Expressing Estrogen Receptor β in the Hypothalamic Nuclei of Rats Using in Situ Hybridization and Immunofluorescence.　　　　　　　　　　　　　　　
  Moeko Kanaya; Shimpei Higo; Hitoshi Ozawa
  International journal of molecular sciences, 巻：21, 号：1, 2019年12月, ［査読有り］, ［国際誌］
  Estrogens play an essential role in multiple physiological functions in the brain, including reproductive neuroendocrine, learning and memory, and anxiety-related behaviors. To determine these estrogen functions, many studies have tried to characterize neurons expressing estrogen receptors known as ERα and ERβ. However, the characteristics of ERβ-expressing neurons in the rat brain still remain poorly understood compared to that of ERα-expressing neurons. The main aim of this study is to determine the neurochemical characteristics of ERβ-expressing neurons in the rat hypothalamus using RNAscope in situ hybridization (ISH) combined with immunofluorescence. Strong Esr2 signals were observed especially in the anteroventral periventricular nucleus (AVPV), bed nucleus of stria terminalis, hypothalamic paraventricular nucleus (PVN), supraoptic nucleus, and medial amygdala, as previously reported. RNAscope ISH with immunofluorescence revealed that more than half of kisspeptin neurons in female AVPV expressed Esr2, whereas few kisspeptin neurons were found to co-express Esr2 in the arcuate nucleus. In the PVN, we observed a high ratio of Esr2 co-expression in arginine-vasopressin neurons and a low ratio in oxytocin and corticotropin-releasing factor neurons. The detailed neurochemical characteristics of ERβ-expressing neurons identified in the current study can be very essential to understand the estrogen signaling via ERβ.
  英語
  DOI：https://doi.org/10.3390/ijms21010115
  DOI ID：10.3390/ijms21010115, PubMed ID：31877966, PubMed Central ID：PMC6981915
• Applicability of Anti-Human Estrogen Receptor β Antibody PPZ0506 for the Immunodetection of Rodent Estrogen Receptor β Proteins.　　　　　　　　　　　　　　　
  Hirotaka Ishii; Mai Otsuka; Moeko Kanaya; Shimpei Higo; Yujiro Hattori; Hitoshi Ozawa
  International journal of molecular sciences, 巻：20, 号：24, 2019年12月, ［査読有り］, ［国際誌］
  Several lines of controversial evidence concerning estrogen receptor β (ERβ) remain to be solved because of the unavailability of specific antibodies against ERβ. The recent validation analysis identified a monoclonal antibody (PPZ0506) with sufficient specificity against human ERβ. However, the specificity and cross-reactivity of PPZ0506 antibody against ERβ proteins from laboratory animals have not been confirmed. In the present study, we aimed to validate the applicability of PPZ0506 to rodent studies. The antibody exhibited specific cross-reactivity against mouse and rat ERβ proteins in immunoblot and immunocytochemical experiments using transfected cells. In immunohistochemistry for rat tissue sections, PPZ0506 showed immunoreactive signals in the ovary, prostate, and brain. These immunohistochemical profiles of rat ERβ proteins in rat tissues accord well with its mRNA expression patterns. Although the antibody was reported to show the moderate signals in human testis, no immunoreactive signals were observed in rat testis. Subsequent RT-PCR analysis revealed that this species difference in ERβ expression resulted from different expression profiles related to the alternative promoter usage between humans and rats. In conclusion, we confirmed applicability of PPZ0506 for rodent ERβ studies, and our results provide a fundamental basis for further examination of ERβ functions.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.3390/ijms20246312
  DOI ID：10.3390/ijms20246312, PubMed ID：31847265, PubMed Central ID：PMC6941125
• Neonatal septal lesions prevent behavioral defeminization caused by neonatal treatment with estradiol in female rats.　　　　　　　　　　　　　　　
  Kanaya M; Tsukahara S; Yamanouchi K
  Neuroscience letters, 巻：694, 開始ページ：80, 終了ページ：85, 2019年02月, ［査読有り］
  DOI：https://doi.org/10.1016/j.neulet.2018.11.032
  DOI ID：10.1016/j.neulet.2018.11.032, ISSN：0304-3940, PubMed ID：30472357
• Temporal expression patterns of genes related to sex steroid action in sexually dimorphic nuclei during puberty　　　　　　　　　　　　　　　
  Moeko Kanaya; Masahiro Morishita; Shinji Tsukahara
  Frontiers in Endocrinology, 巻：9, 号：MAY, 開始ページ：213, 2018年05月, ［査読有り］
  Sex steroids play a major role in sexually dimorphic brain development during not only the perinatal period but also the pubertal period. We previously showed that, in male mice, the estrogen receptor-a (Esr1) and aromatase (Cyp19α1) genes are essential to the sexually dimorphic formation of the anteroventral periventricular nucleus (AVPV) and the principal nucleus of the bed nucleus of the stria terminalis (BNSTp), but the estrogen receptor-β (Esr2) gene is not necessary. We also showed that the androgen receptor (Ar) gene is essential to the sexually dimorphic formation of the BNSTp. These genes are expressed in the AVPV and BNSTp of perinatal mice. However, it remains unknown whether these genes are expressed in the AVPV and BNSTp during puberty, and whether the expression, if any, differs by sex, age, and brain region. Here, we dissected the AVPV and BNSTp from Nissl-stained brain sections of male and female mice on postnatal day (PD) 20 (prepuberty), PD30 (puberty onset in females), PD40 (puberty onset in males), and PD60 (young adult) using a laser microdissection system. We then examined the mRNA levels of Esr1, Esr2, Cyp19α1, and Ar in these brain regions. In the AVPV, Esr1 mRNA levels were greater in females than males during PD20-60. Esr2 and Ar mRNA expressions did not differ between sexes. Ar mRNA levels were higher at PD30 than PD20. Cyp19α1 mRNA was not detected in the AVPV at PD20-60. In the BNSTp, Esr1 and Esr2 mRNA levels were higher in females than in males during PD20-60, although the mRNA levels of Cyp19α1 and Ar did not differ between sexes. Additionally, we revealed that orchiectomy at PD20 induced a failure of normal formation of the male BNSTp and testosterone replacement in the prepubertal period rescued the effect of orchiectomy at PD20. Taken together, it is suggested that pubertal testosterone transported to the AVPV is not converted to estradiol there and does not act via ESR1 and ESR2. By contrast, the formation of the male BNSTp may be affected by testicular testosterone during puberty via AR and/or via ESR1 after conversion to estradiol by CYP19A1.
  Frontiers Media S.A., 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.3389/fendo.2018.00213
  DOI ID：10.3389/fendo.2018.00213, ISSN：1664-2392, PubMed ID：29770127, SCOPUS ID：85046672261
• Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Kinuyo Iwata; Hitoshi Ozawa
  BIOLOGY OF REPRODUCTION, 巻：97, 号：5, 開始ページ：709, 終了ページ：718, 2017年11月, ［査読有り］
  Kisspeptin (KISS1; encoded by Kiss1) neurons in the arcuate nucleus (ARC) coexpress tachykinin 3 (TAC3; also known as neurokinin B) and dynorphin A (PDYN). Accordingly, they are termed KNDy neurons and considered to be crucial in generating pulsatile release of gonadotropin-releasing hormone. Accumulating evidence suggests that Kiss1 and Tac3 are negatively regulated by estrogen. However, it has not been fully determined whether and how estrogen modulates Pdyn and PDYN. Here, we examined the expression of Pdyn mRNA and PDYN by in situ hybridization and immunohistochemistry, respectively, in the ARC of female rats after ovariectomy (OVX) and OVX plus low-or high-dose beta-estradiol (E-2) replacement. We also investigated the effect of E-2 on expression of Kiss1, KISS1, Tac3, and TAC3. Furthermore, colocalization of PDYN and estrogen receptor alpha (ESR1) was determined. Subsequently, we found that low-dose E-2 treatment had no effect on Pdyn mRNA-expressing cells, but increased PDYN-immunoreactive (ir) cell numbers. In contrast, high-dose E-2 treatment resulted in prominent reductions in both Pdyn mRNA-expressing and PDYN-ir cell numbers. Changes induced by low or high doses of E-2 were similarly observed in the expression of Kiss1, KISS1, Tac3, and TAC3. The majority of PDYN-ir neurons coexpressed ESR1 in all groups. Our results indicate that E-2 regulates the expression of PDYN, as well as KISS1 and TAC3, with regulation by E-2 differing according to its levels.
  Summary Sentence
  Estrogen regulates dynorphin expression in the arcuate nucleus of female rats with low-dose estrogen suppressing dynorphin release and high-dose estrogen inhibiting dynorphin mRNA expression.
  OXFORD UNIV PRESS INC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1093/biolre/iox131
  DOI ID：10.1093/biolre/iox131, ISSN：0006-3363, eISSN：1529-7268, PubMed ID：29069289, Web of Science ID：WOS:000418963100006
• Regional Difference in Sex Steroid Action on Formation of Morphological Sex Differences in the Anteroventral Periventricular Nucleus and Principal Nucleus of the Bed Nucleus of the Stria Terminalis　　　　　　　　　　　　　　　
  Moeko Kanaya; Mumeko C. Tsuda; Shoko Sagoshi; Kazuyo Nagata; Chihiro Morimoto; Chaw Kyi Tha Thu; Katsumi Toda; Shigeaki Kato; Sonoko Ogawa; Shinji Tsukahara
  PLOS ONE, 巻：9, 号：11, 開始ページ：e112616, 2014年11月, ［査読有り］
  Sex steroid action is critical to form sexually dimorphic nuclei, although it is not fully understood. We previously reported that masculinization of the principal nucleus of the bed nucleus of the stria terminalis (BNSTp), which is larger and has more neurons in males than in females, involves aromatized testosterone that acts via estrogen receptor-alpha (ER alpha), but not estrogen receptor-beta (ER beta). Here, we examined sex steroid action on the formation of the anteroventral periventricular nucleus (AVPV) that is larger and has more neurons in females. Morphometrical analysis of transgenic mice lacking aromatase, ER alpha, or ER beta genes revealed that the volume and neuron number of the male AVPV were significantly increased by deletion of aromatase and ER alpha genes, but not the ERb gene. We further examined the AVPV and BNSTp of androgen receptor knockout (ARKO) mice. The volume and neuron number of the male BNSTp were smaller in ARKO mice than those in wild-type mice, while no significant effect of ARKO was found on the AVPV and female BNSTp. We also examined aromatase, ER alpha, and AR mRNA levels in the AVPV and BNSTp of wild-type and ARKO mice on embryonic day (ED) 18 and postnatal day (PD) 4. AR mRNA in the BNSTp and AVPV of wild-type mice was not expressed on ED18 and emerged on PD4. In the AVPV, the aromatase mRNA level was higher on ED18, although the ER alpha mRNA level was higher on PD4 without any effect of AR gene deletion. Aromatase and ER alpha mRNA levels in the male BNSTp were significantly increased on PD4 by AR gene deletion. These results suggest that estradiol signaling via ERa during the perinatal period and testosterone signaling via AR during the postnatal period are required for masculinization of the BNSTp, whereas the former is sufficient to defeminize the AVPV.
  PUBLIC LIBRARY SCIENCE, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1371/journal.pone.0112616
  DOI ID：10.1371/journal.pone.0112616, ISSN：1932-6203, PubMed ID：25398007, Web of Science ID：WOS:000345558500066
• Neuroanatorny and sex differences of the lordosis-inhibiting system in the lateral septum　　　　　　　　　　　　　　　
  Shinji Tsukahara; Moeko Kanaya; Korehito Yamanouchi
  FRONTIERS IN NEUROSCIENCE, 巻：8, 開始ページ：299, 2014年09月, ［査読有り］
  Female sexual behavior in rodents, termed lordosis, is controlled by facilitatory and inhibitory systems in the brain. It has been well demonstrated that a neural pathway from the ventromedial hypothalamic nucleus (VMN) to the midbrain central gray (MCG) is essential for facilitatory regulation of lordosis. The neural pathway from the arcuate nucleus to the VMN, via the medial preoptic nucleus, in female rats mediates transient suppression of lordosis, until female sexual receptivity is induced. In addition to this pathway, other regions are involved in inhibitory regulation of lordosis in female rats. The lordosis-inhibiting systems exist not only in the female brain but also in the male brain. The systems contribute to suppression of heterotypical sexual behavior in male rats, although they have the potential ability to display lordosis. The lateral septum (LS) exerts an inhibitory influence on lordosis in both female and male rats. This review focuses on the neuroanatomy and sex differences of the lordosis-inhibiting system in the LS. The LS functionally and anatomically links to the MCG to exert suppression of lordosis. Neurons of the intermediate part of the LS (LSi) serve as lordosis-inhibiting neurons and project axons to the MCG. The LSi-MCG neural connection is sexually dimorphic, and formation of the male-like LSi-MCG neural connection is affected by aromatized testosterone originating from the testes in the postnatal period. The sexually dimorphic LSi-MCG neural connection may reflect the morphological basis of sex differences in the inhibitory regulation of lordosis in rats.
  FRONTIERS RESEARCH FOUNDATION, 英語
  DOI：https://doi.org/10.3389/fnins.2014.00299
  DOI ID：10.3389/fnins.2014.00299, ISSN：1662-453X, Web of Science ID：WOS:000346527100001
• Involvement of hemeoxygenase-1 in di(2-ethylhexyl) phthalate (DEHP)-induced apoptosis of Neuro-2a cells　　　　　　　　　　　　　　　
  Kyaw Htet Aung; Tin-Tin Win-Shwe; Moeko Kanaya; Hirohisa Takano; Shinji Tsukahara
  JOURNAL OF TOXICOLOGICAL SCIENCES, 巻：39, 号：2, 開始ページ：217, 終了ページ：229, 2014年04月, ［査読有り］
  A widely-used plasticizer di(2-ethylhexyl) phthalate (DEHP) is known to induce apoptosis in neurons, although the mechanisms responsible for DEHP-induced apoptosis is not well explored yet. We recently showed that exposure to DEHP increases the expression of hemeoxygenase (HO)-1, an oxidative stress related enzyme, in the mice brain. In this study, we investigated whether HO-1 is involved in DEHP-induced apoptosis using a mouse neuroblastoma cell line Neuro-2a, which forcibly express SCAT3, a fluorescent indicator of caspase-3 activity. The doses of DEHP at 1, 10 or 100 mu M were used in the present study to mimic the level of human exposure to DEHP. Live image analysis of SCAT3-expressing Neuro-2a cells revealed that caspase-3 activity in the cells was significantly increased by DEHP at 100 mu M but not 1 or 10 mu M. We measured HO-1 mRNA level in Neuro-2a cells exposed to DEHP and found significant increase in HO-1 mRNA level by DEHP at 100 mu M but not 1 or 10 mu M. Live image analysis of SCAT3-expresisng Neuro-2a cells was further performed to determine the effects of HO-1 siRNA in DEHP-induced apoptosis via caspase-3 activation. We found that knockdown of HO-1 gene nullifies the effects of DEBT to activate caspase-3. These results suggest that HO-1 is involved in DEHP-induced apoptosis. Moreover, this study demonstrates that high-dose DEHP exposure induces caspase-3-dependent apoptosis, which is at least partially mediated by the up-regulation of HO-1 gene, in Neuro-2a cells.
  JAPANESE SOC TOXICOLOGICAL SCIENCES, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2131/jts.39.217
  DOI ID：10.2131/jts.39.217, ISSN：0388-1350, eISSN：1880-3989, PubMed ID：24646702, Web of Science ID：WOS:000334013300005
• Defeminization of brain functions by a single injection of estrogen receptor α or β agonist in neonatal female rats.　　　　　　　　　　　　　　　
  Kanaya M; Yamanouchi K
  Neuroendocrinology, 巻：95, 号：4, 開始ページ：297, 終了ページ：304, 2012年06月, ［査読有り］
  Sexual differentiation of brain function is regulated by estrogen in the perinatal period of rodents. However, the role of the estrogen receptor subtypes ERα and ERβ is still in question. Accordingly, the effects of neonatal treatment with the ERα agonist propyl pyrazole triol (PPT) or the ERβ agonist diarylpropionitrile (DPN) on female reproductive functions were investigated in rats. Female rats were injected subcutaneously with 100-500 μg/10 g body weight (b.w.) PPT or DPN, 100 μg/10 g b.w. estradiol (E 2), or saline at day 5 (birth day = day 1), and then vaginal opening and vaginal smears were examined. On day 60, their ovaries were removed and lordosis behavior was observed after subcutaneous implantation of a silicon tube containing E 2. As a result, in most PPT and all E 2 rats, vaginal opening was advanced and an irregular estrous cycle was observed. In contrast, in most rats of the DPN groups, vaginal opening was comparable to that of the control and there was a regular estrous cycle. Lordosis tests revealed that the mean lordosis quotients (LQs) in the 250- and 500-μg PPT groups was lower than in the saline group, but higher than in the E 2 group. Mean LQs in all DPN groups were comparable to those in the saline group. These results suggest that ERα plays a major role in masculinization of the system regulating the estrous cycle in the rat brain. In behavioral defeminization of the lordosis-regulation system, ERα was also found to be the main target of estrogen. Copyright © 2012 S. Karger AG.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1159/000332128
  DOI ID：10.1159/000332128, ISSN：0028-3835, PubMed ID：22327340, SCOPUS ID：84862775880

• 【形態学の逆襲:形態学教室の扉をたたいてみてください】In situ hybridization法の高感度化手法とその神経科学未解明領域への応用　　　　　　　　　　　　　　　
  肥後 心平; 金谷 萌子; 水野 友喜; 小澤 一史; 坂本 篤裕; 石井 寛高
  日本医科大学医学会雑誌, 巻：19, 号：2, 開始ページ：84, 終了ページ：89, 2023年04月
  日本医科大学医学会, 日本語
  ISSN：1349-8975, eISSN：1880-2877, 医中誌Web ID：X526360003

• 慢性疼痛モデルマウスの免疫細胞遺伝子発現に対する性ホルモンの影響　　　　　　　　　　　　　　　
  金谷萌子; 髙橋友紀乃
  第50回日本神経内分泌学会学術集会, 2024年10月
• 痛み行動の性差に対する新生期の性ステロイド処置の影響　　　　　　　　　　　　　　　
  金谷萌子; 宮田麻理子
  第44回日本神経科学大会, 2021年07月, ［国内会議］
  日本語, ポスター発表, 神戸, 日本国
• 抗ヒトERβ特異的モノクローナル抗体(PPZ0506)を用いたラット組織に対する免疫組 織化学的検出方法の最適化　　　　　　　　　　　　　　　
  服裕次郎; 石井寛高; 肥後心平; 大塚真衣; 金谷萌子; 松本恵介; 呉立洋; 小澤実那; 小澤一史
  第61回日本組織細胞化学会総会・学術集会, 2020年12月, ［国内会議］
  日本語, ポスター発表, WEB開催, 日本国
• 齧歯類における抗ヒトERβ抗体(PPZ0506)の交差性・特異性検証とERβ発現分布解析　　　　　　　　　　　　　　　
  大塚真衣; 石井寛高; 金谷萌子; 肥後心平; 小澤一史
  第125回日本解剖学会総会・全国学術集会, 2020年03月, ［国内会議］
  日本語, ポスター発表, 誌上開催, 日本国
• 抗ヒトERβ抗体（PPZ0506）のマウス・ラットERβに対する交差性・特異性の検証とERβ発現プロファイルの同定　　　　　　　　　　　　　　　
  大塚真衣; 石井寛高; 金谷萌子; 肥後心平; 小澤一史
  第60回日本組織細胞化学会総会・学術集会, 2019年09月, ［国内会議］
  日本語, ポスター発表, 神戸, 日本国
• 女性研究者セッション～組織細胞化学研究の魅力を語る～生殖機能制御を中心としたこれまでの研究について　　　　　　　　　　　　　　　
  岩田衣世; 金谷萌子; 國村有弓; 村川裕子; 小澤一史
  第60回日本組織細胞化学会総会, 2019年09月, ［国内会議］
  日本語, シンポジウム・ワークショップパネル（指名）, 神戸, 日本国
• ラットの視床下部神経核におけるエストロゲン受容体βの神経化学特性の同定　　　　　　　　　　　　　　　
  金谷萌子; 肥後心平; 小澤一史
  第124回日本解剖学会総会・学術集会, 2019年03月, ［国内会議］
  日本語, ポスター発表, 新潟, 日本国
• Neurochemical characterization of neurons expressing estrogen receptor-beta in the hypothalamus of rats using RNAscope in situ hybridization and immunofluoresecence　　　　　　　　　　　　　　　
  Moeko Kanaya; Shimpei Higo; Hitoshi Ozawa
  10th Internationalmeeting Steroids and Nervous System, 2019年02月, ［国際会議］
  英語, ポスター発表, Torino, Italy, イタリア共和国
• 新たなin situ hybridization法によるエストロゲン受容体βの発現解析　　　　　　　　　　　　　　　
  金谷萌子; 肥後心平; 小澤一史
  第59回日本組織細胞化学会総会・学術集会, 2018年09月, ［国内会議］
  日本語, 口頭発表（一般）, 宮崎, 日本国
• 性的二型核の性差形成における性ステロイドの作用機構　　　　　　　　　　　　　　　
  金谷萌子
  第29回日本行動神経内分泌研究会, 2018年09月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）, 相模原, 日本国
• エストロゲンの濃度変化における雌ラット弓状核dynorphinの発現変動　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  第122回日本解剖学会総会・全国学術集会, 2017年03月, ［国内会議］
  日本語, ポスター発表, 長崎, 日本国
• 雌ラット弓状核のdynorphin発現に対するエストロゲンのネガティブフィードバック制御　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  第43回日本神経内分泌学会学術集会, 2016年10月, ［国内会議］
  日本語, 口頭発表（一般）, 浜松, 日本国
• エストロゲンの濃度変化による弓状核dynorphinの発現変動　　　　　　　　　　　　　　　
  金谷萌子; 岩田衣世; 小澤一史
  第25回日本行動神経内分泌研究会, 2016年09月, ［国内会議］
  日本語, 口頭発表（一般）, 熱海, 日本国
• 性的二型核の性差形成における周生期と思春期の性ステロイド作用　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; Chaw Kyi Tha Thu; 戸田勝巳; 加藤茂明; 小川園子; 塚原伸治
  第42回日本神経内分泌学会・第23回日本行動神経内分泌研究会合同学術集会, 2015年09月, ［国内会議］
  日本語, 口頭発表（一般）, 仙台, 日本国
• 性行動と脳構造の性分化における性ステロイドホルモンの役割　　　　　　　　　　　　　　　
  金谷萌子
  第20回「性と生殖」公開シンポジウム：人間科学における神経内分泌研究からの展開, 2014年12月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）, 東京, 日本国
• 前腹側脳室周囲核と分界条床核主核の性差形成に対する性ステロイド作用の相違性と類似性　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; Chaw Kyi Tha Thu; 戸田勝巳; 加藤茂明; 小川園子; 塚原伸治
  第37回日本神経科学会, 2014年09月, ［国内会議］
  日本語, ポスター発表, 横浜, 日本国
• Regional differences in sexual steroid action on formation of sexually dimorphic nuclei in mice　　　　　　　　　　　　　　　
  Moeko Kanaya; Mumeko C. Tsuda; Shoko Sagoshi; Kazuyo Nagata; Chihiro Morimoto; Chaw Kyi Tha Thu; Katsumi Toda; Shigeaki Kato; Sonoko Ogawa; Shinji Tsukahara
  The 8th International Congress of Neuroendocrinology 2014, 2014年08月, ［国際会議］
  英語, ポスター発表, Sydney, Australia, オーストラリア連邦
• マウスの性的二型核形成に関与する性ステロイド作用の部位差　　　　　　　　　　　　　　　
  金谷萌子; 津田夢芽子; 佐越祥子; 永田知代; 森本千尋; 栗原良平; 加藤茂明; 小川園子; 塚原伸治
  日本動物学会第84回大会, 2013年09月, ［国内会議］
  日本語, 口頭発表（一般）, 岡山, 日本国
• Involvement of the septum on neonatal estrogen-induced behavioral defeminization in rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Shinji Tsukahara; Korehito Yamanouchi
  Neuroscience 2012, 2012年10月, ［国際会議］
  英語, ポスター発表, New Orleans, USA, アメリカ合衆国
• 雌ラット新生期中隔破壊は新生期エストロゲン投与による行動の脱雌性化を阻止するが無排卵症候群は阻止しない　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  第35回日本神経科学会, 2012年09月, ［国内会議］
  日本語, ポスター発表, 名古屋, 日本国
• 雄ラットのロードーシス発現に対する新生期中隔破壊効果　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  日本動物学会第83回大会, 2012年09月, ［国内会議］
  日本語, 口頭発表（一般）, 大阪, 日本国
• 性行動の脱雌性化に対する新生期中隔破壊の効果　　　　　　　　　　　　　　　
  金谷萌子; 塚原伸治; 山内兄人
  第17回日本神経行動内分泌研究会, 2012年08月, ［国内会議］
  日本語, 口頭発表（一般）, 京都, 日本国
• 雌ラットの脳機能の雄性化に対するエストロゲンα, β受容体作動剤新生期投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  第16回日本生殖内分泌学会学術集会, 2011年11月, ［国内会議］
  日本語, 口頭発表（一般）, 東京, 日本国
• 雌ラット視床下部ERα免疫陽性細胞数へのERα, β作動剤, 性ステロイド投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  日本動物学会第82回大会, 2011年09月, ［国内会議］
  日本語, ポスター発表, 旭川, 日本国
• Effects of ERα and β agonists, estrogen and androgen on number of ERα-immunoreactive cells in the hypothalamus of female rats　　　　　　　　　　　　　　　
  Moeko Kanaya; Ayako Ogata; Korehito Yamanouchi
  8th IBRO World Congress of Neuroscience, 2011年07月, ［国際会議］
  英語, ポスター発表, Florence, Italy, イタリア共和国
• 雌ラットの性周期とロードーシスに対するエストロゲンα, β受容体作動剤新生期投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  第15回日本行動神経内分泌研究会, 2011年06月, ［国内会議］
  日本語, 口頭発表（一般）, 東京, 日本国
• 雌ラットにおける性周期とロードーシスに対するエストロゲンα, β受容体作動剤投与効果　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  第33回日本神経科学会, 2010年09月, ［国内会議］
  日本語, ポスター発表, 神戸, 日本国
• 雌ラット発情周期とロードーシスに対するエストロゲン受容体α, β作動剤新生期投与　　　　　　　　　　　　　　　
  金谷萌子; 山内兄人
  日本動物学会第81回大会, 2010年09月, ［国内会議］
  日本語, 口頭発表（一般）, 東京, 日本国
• Defeminizing and masculinizing effects of single injection with estrogen receptor α or β agonist in neonatal female rat　　　　　　　　　　　　　　　
  Moeko Kanaya; Korehito Yamanouchi
  The 7th International Congress of Neuroendocrinology, 2010年07月, ［国際会議］
  英語, ポスター発表, Rouen, France, フランス共和国

• 日本組織細胞化学会
• 日本神経科学学会
• 日本神経内分泌学会
• 日本生理学会

• 痛みの性差を引き起こす神経回路に関する研究　　　　　　　　　　　　　　　
  一般財団法人フォーデイズ自立支援協会研究助成金, 2024年07月 - 2025年06月
  研究代表者
• 痛みの性差をもたらす痛覚の神経回路メカニズムの解明　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 若手研究, 2021年04月 - 2024年03月
  金谷 萌子, 東京女子医科大学
  配分額（総額）：4680000, 配分額（直接経費）：3600000, 配分額（間接経費）：1080000
  課題番号：21K15345
• 「女脳」「男脳」を科学する　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 2020年07月30日 - 2023年03月31日
  宮田 麻理子; 金谷 萌子, 東京女子医科大学
  配分額（総額）：6500000, 配分額（直接経費）：5000000, 配分額（間接経費）：1500000
  課題番号：20K21508
• 環境情報入力との連関を基盤とする新規生殖機能制御系に関する分子機能形態学的解析　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 2018年04月01日 - 2022年03月31日
  小澤 一史; 岩田 衣世; 金谷 萌子; 石井 寛高; 服部 裕次郎; 肥後 心平; 渡辺 雄貴, 日本医科大学
  配分額（総額）：4420000, 配分額（直接経費）：3400000, 配分額（間接経費）：1020000
  生殖制御系の新しい「主役」であるKisspeptinとその含有ニューロンの機能形態学に関して組織細胞化学的手法、分子生物学的研究手法等を駆使して研究展開を行った。その結果、エネルギー代謝と生殖制御神経軸との関連性、性ホルモンのsubtypeとその機能、kisspeptin関連ペプチドの脳内分布とその機能、Dohad theoryに基づく母胎内環境の生後に及ぼす影響、糖尿病モデル動物における生殖制御機構の変化、kisspepニューロンへの影響など幅広い研究業績を上げることが出来た。
  課題番号：18K06860
• 発達期の性ステロイドホルモンによって規定される脳機能の性差に関する研究　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 特別研究員奨励費, 2019年04月 - 2022年03月
  金谷 萌子, 東京女子医科大学
  配分額（総額）：4030000, 配分額（直接経費）：3100000, 配分額（間接経費）：930000
  課題番号：19J00621
• 産後うつ病におけるうつ様行動関連脳領域に対する遺伝的背景の影響　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 若手研究, 2018年04月 - 2021年03月
  金谷 萌子
  配分額（総額）：3250000, 配分額（直接経費）：2500000, 配分額（間接経費）：750000
  課題番号：18K16818