Performance information

• Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels
  Saku T. Kijima; Takema Sasaki; Yuichiro Kikushima; Daisuke Inoue; Shingo Sakamoto; Yuki Kondo; Soichi Inagaki; Masatoshi Yamaguchi; Nobutaka Mitsuda; Yoshihisa Oda
  Nature Communications, Volume：16, Number：1, Feb. 2025, [Reviewed]
  Springer Science and Business Media LLC, English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-025-56866-y
  DOI ID：10.1038/s41467-025-56866-y, eISSN：2041-1723
• The deficiency of methylglyoxal synthase promotes cell proliferation in Synechocystis sp. PCC 6803 under mixotrophic conditions　　　　　　　　　　　　　　　
  Aikeranmu Kadeer; Yuuma Ishikawa; Kaushalya Dayarathne; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Plant Biotechnology, Volume：41, Number：4, First page：393, Last page：399, Dec. 2024, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.24.0718a
  DOI ID：10.5511/plantbiotechnology.24.0718a
• The effect of light availability and light wavelength on growth, 2-MIB biosynthesis, and 2-MIB-related gene expression in Pseudanabaena foetida var. intermedia.　　　　　　　　　　　　　　　
  Kaushalya Dayarathne; Toshiki Ishikawa; Aikeranmu Kadeer; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Archives of microbiology, Volume：206, Number：9, First page：367, Last page：367, Aug. 2024, [Reviewed], ［International magazine］
  2-methylisoborneol (2-MIB) is an odiferous metabolite mainly produced by cyanobacteria, contributing to taste and odor problems in drinking water. The mechanisms involved in 2-MIB biosynthesis in cyanobacteria are not yet completely understood. This study investigated the effect of light availability and wavelength on growth, 2-MIB synthesis, and related gene expression in Pseudanabaena foetida var. intermedia. A significantly lower 2-MIB production was observed in P. foetida var. intermedia during the dark period of a 12-h photoperiod. Exposure to green light resulted in a significant decrease in 2-MIB production compared to white light and red light. The relative expression levels of 2-MIB-related genes in P. foetida var. intermedia were significantly lower during the dark period of a 12-h photoperiod and when cultured under green light. The expression of 2-MIB-related genes in cyanobacteria appears to be light-dependent. This study suggests that the demand for photopigment synthesis under unfavorable light conditions affects the 2-MIB synthesis in cyanobacteria.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00203-024-04099-w
  DOI ID：10.1007/s00203-024-04099-w, PubMed ID：39105810
• Heterologous expression of mtf and mtc genes of Pseudanabaena foetida var. intermedia is sufficient to produce 2-methylisoborneol in Escherichia coli.　　　　　　　　　　　　　　　
  Kaushalya Dayarathne; Toshiki Ishikawa; Satoru Watanabe; Yuuma Ishikawa; Kadeer Aikeranmu; Hina Kitagawaa; Natsumi Komatsubara; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Microbiology spectrum, Volume：11, Number：5, First page：e0256123, Sep. 2023, [Reviewed], ［International magazine］
  Microbial volatile metabolite 2-methylisoborneol (2-MIB) causes odor and taste issues in drinking water, making it unappealing for human consumption. It has been suggested that 2-MIB biosynthesis consists of two main steps, namely, methylation of geranyl diphosphate into 2-methyl geranyl diphosphate by geranyl diphosphate methyl transferase (GPPMT) and subsequent cyclization into 2-MIB by 2-MIB synthase (MIBS). Pseudanabaena foetida var. intermedia is a 2-MIB-producing cyanobacterium whose GPPMT and MIBS enzymes are encoded by adjacent mtf and mtc genes. The present study identified a 2-MIB-related gene cluster composed of cnbA, mtf, mtc, and cnbB genes in P. foetida var. intermedia. The two homologous cyclic nucleotide-binding protein genes, cnbA and cnbB, were detected adjacent to the mtf and mtc genes, respectively. The nucleotide sequence of the cnbA-mtf-mtc-cnbB gene cluster showed 99.55% identity with 2-MIB synthesis-associated gene cluster of Pseudanabaena sp. dqh15. RT-PCR results revealed that mtf and mtc genes are co-expressed, while cnbA and cnbB genes are expressed independently in P. foetida var. intermedia. To investigate whether only mtf and mtc genes are sufficient for 2-MIB synthesis, the two-gene unit (mtf-mtc) was introduced into Escherichia coli strain JM109 via overexpression vector pYS1C. Gas chromatograph-mass spectrometry results showed that the E. coli strain transformed with mtf-mtc was able to produce 2-MIB. The intracellular 2-MIB level in P. foetida var. intermedia was higher than the extracellular 2-MIB level, while the transformed E. coli strain showed an opposite trend. Growth inhibition was observed in the 2-MIB-producing transformed E. coli strain. IMPORTANCE Contamination of drinking water with odiferous microbial metabolite 2-MIB is a worldwide concern. Removal of 2-MIB from drinking water burdens the water purification process. Therefore, it is important to search for alternative methods, such as suppressing the production of 2-MIB by aquatic microorganisms. For that, it is necessary to expand the current knowledge about the mechanism of 2-MIB synthesis at the genetic level. This study revealed that mtf and mtc genes of the 2-MIB-related gene cluster are transcribed as a single unit in P. foetida var. intermedia, and the expression of both mtf and mtc genes is essential and sufficient for 2-MIB synthesis in E. coli heterologous gene expression system.
  English, Scientific journal
  DOI：https://doi.org/10.1128/spectrum.02561-23
  DOI ID：10.1128/spectrum.02561-23, PubMed ID：37732762, PubMed Central ID：PMC10580876
• NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence
  Isura Sumeda Priyadarshana Nagahage; Kohei Matsuda; Kyoko Miyashita; Sumire Fujiwara; Chanaka Mannapperuma; Takuya Yamada; Shingo Sakamoto; Toshiki Ishikawa; Minoru Nagano; Misato Ohtani; Ko Kato; Hirofumi Uchimiya; Nobutaka Mitsuda; Maki Kawai‐Yamada; Taku Demura; Masatoshi Yamaguchi
  Plant Direct, Volume：7, Number：9, Sep. 2023, [Reviewed], [Last, Corresponding]
  Abstract
  
  The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two‐hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1002/pld3.529
  DOI ID：10.1002/pld3.529, ISSN：2475-4455, eISSN：2475-4455
• Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants　　　　　　　　　　　　　　　
  Chaomurilege; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Hideki Murayama; Maki Kawai-Yamada
  Plant Signaling and Behavior, Volume：18, Number：1, First page：e2215618, May 2023, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1080/15592324.2023.2215618
  DOI ID：10.1080/15592324.2023.2215618
• Loss of peroxisomal NAD kinase 3 (NADK3) affects photorespiration metabolism in Arabidopsis　　　　　　　　　　　　　　　
  Shota Suzuki; Daimu Tanaka; Atsuko Miyagi; Kentaro Takahara; Masaru Kono; Chaomurilege; Ko Noguchi; Toshiki Ishikawa; Minoru Nagano; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Journal of Plant Physiology, Volume：283, First page：153950, Mar. 2023, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2023.153950
  DOI ID：10.1016/j.jplph.2023.153950
• Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ
  Atsuko Miyagi; Kazuhisa Mori; Toshiki Ishikawa; Satoshi Ohkubo; Shunsuke Adachi; Masatoshi Yamaguchi; Taiichiro Ookawa; Toshihisa Kotake; Maki Kawai-Yamada
  Metabolomics, Volume：18, Number：12, Nov. 2022, [Reviewed]
  Springer Science and Business Media LLC, Scientific journal
  DOI：https://doi.org/10.1007/s11306-022-01958-9
  DOI ID：10.1007/s11306-022-01958-9, eISSN：1573-3890
• Loss of chloroplast-localized NAD kinase causes ROS stress in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Chaomurilege; Yanhui Zu; Atsuko Miyagi; Shin-Nosuke Hashida; Toshiki Ishikawa; Masatoshi Yamaguchi; Maki Kawai-Yamada
  Journal of Plant Research, Volume：in press, Nov. 2022, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-022-01420-w
  DOI ID：10.1007/s10265-022-01420-w
• Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst
  Tomomi Ukawa; Fumihiko Banno; Toshiki Ishikawa; Kota Kasahara; Yuuta Nishina; Rika Inoue; Keigo Tsujii; Masatoshi Yamaguchi; Takuya Takahashi; Yoichiro Fukao; Maki Kawai-Yamada; Minoru Nagano
  Plant Physiology, Volume：189, Number：2, First page：839, Last page：857, Jun. 2022, [Reviewed]
  Abstract
  
  Plant sphingolipids mostly possess 2-hydroxy fatty acids (HFA), the synthesis of which is catalyzed by FA 2-hydroxylases (FAHs). In Arabidopsis (Arabidopsis thaliana), two FAHs (FAH1 and FAH2) have been identified. However, the functions of FAHs and sphingolipids with HFAs (2-hydroxy sphingolipids) are still unknown because of the lack of Arabidopsis lines with the complete deletion of FAH1. In this study, we generated a FAH1 mutant (fah1c) using CRISPR/Cas9-based genome editing. Sphingolipid analysis of fah1c, fah2, and fah1cfah2 mutants revealed that FAH1 hydroxylates very long-chain FAs (VLCFAs), whereas the substrates of FAH2 are VLCFAs and palmitic acid. However, 2-hydroxy sphingolipids are not completely lost in the fah1cfah2 double mutant, suggesting the existence of other enzymes catalyzing the hydroxylation of sphingolipid FAs. Plasma membrane (PM) analysis and molecular dynamics simulations revealed that hydroxyl groups of sphingolipid acyl chains play a crucial role in the organization of nanodomains, which are nanoscale liquid-ordered domains mainly formed by sphingolipids and sterols in the PM, through hydrogen bonds. In the PM of the fah1cfah2 mutant, the expression levels of 26.7% of the proteins, including defense-related proteins such as the pattern recognition receptors (PRRs) brassinosteroid insensitive 1-associated receptor kinase 1 and chitin elicitor receptor kinase 1, NADPH oxidase respiratory burst oxidase homolog D (RBOHD), and heterotrimeric G proteins, were lower than that in the wild-type. In addition, reactive oxygen species (ROS) burst was suppressed in the fah1cfah2 mutant after treatment with the pathogen-associated molecular patterns flg22 and chitin. These results indicated that 2-hydroxy sphingolipids are necessary for the organization of PM nanodomains and ROS burst through RBOHD and PRRs during pattern-triggered immunity.
  Oxford University Press (OUP), English, Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiac134
  DOI ID：10.1093/plphys/kiac134, ISSN：0032-0889, eISSN：1532-2548
• VNI2 effectively inhibits transcriptional activities of VND7 through a conserved sequence　　　　　　　　　　　　　　　
  Aili Ailizati; Isura Sumeda Priyadarshana Nagahage; Atsuko Miyagi; Toshiki Ishikawa; Maki Kawai-Yamada; Taku Demura; Masatoshi Yamaguchi
  Plant Biotechnology, Volume：39, Number：2, First page：147, Last page：153, Jun. 2022, [Reviewed], [Last, Corresponding], ［Internationally co-authored］
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.22.0122a
  DOI ID：10.5511/plantbiotechnology.22.0122a
• An Arabidopsis NAC domain transcriptional activator VND7 negatively regulates VNI2 expression.　　　　　　　　　　　　　　　
  Ailizati A; Nagahage ISP; Miyagi A; Ishikawa T; Kawai-Yamada M; Demura T; Yamaguchi M
  Plant Biotechnology, First page：in press, Oct. 2021, [Reviewed], [Last, Corresponding]
• Change in expression levels of NAD kinase-encoding genes in Flaveria species　　　　　　　　　　　　　　　
  Masami Tanaka; Yuuma Ishikawa; Sayaka Suzuki; Takako Ogawa; Yukimi Y. Taniguchi; Atsuko Miyagi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yuri N. Munekage; Maki Kawai-Yamada
  Journal of Plant Physiology, Volume：265, First page：153495, Last page：153495, Oct. 2021, [Reviewed], ［International magazine］
  Nicotinamide adenine dinucleotides (NAD(H)) and NAD phosphates (NADP(H)) are electron carriers involved in redox reactions and metabolic processes in all organisms. NAD kinase (NADK) is the only enzyme that phosphorylates NAD+ into NADP+, using ATP as a phosphate donor. In NADP-dependent malic enzyme (NADP-ME)-type C4 photosynthesis, NADP(H) are required for dehydrogenation by NADP-dependent malate dehydrogenase (NADP-MDH) in mesophyll cells, and decarboxylation by NADP-ME in bundle sheath cells. In this study, we identified five NADK genes (FbNADK1a, 1b, 2a, 2b, and 3) from the C4 model species Flaveria bidentis. RNA-Seq database analysis revealed higher transcript abundance in one of the chloroplast-type NADK2 genes of C4F. bidentis (FbNADK2a). Comparative analysis of NADK activity in leaves of C3, C3-C4, and C4Flaveria showed that C4Flaveria (F. bidentis and F. trinervia) had higher NADK activity than the other photosynthetic-types of Flaveria. Taken together, our results suggest that chloroplastic NAD kinase appeared to increase in importance as C3 plants evolved into C4 plants in the genus Flaveria.
  Elsevier BV, English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2021.153495
  DOI ID：10.1016/j.jplph.2021.153495, ISSN：0176-1617, PubMed ID：34411985
• The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Cedric Cassan; Aikeranmu Kadeer; Koki Yuasa; Nozomu Sato; Kintake  Sonoike; Yasuko Kaneko; Atsuko Miyagi; Hiroko Takahashi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yoshitaka Nishiyama; Yukako Hihara; Yves Gibon; Maki Kawai-Yamada
  Plant and Cell Physiology, Volume：62, Number：4, First page：668, Last page：677, Sep. 2021, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab023
  DOI ID：10.1093/pcp/pcab023, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：33560438, Web of Science ID：WOS:000728395500015
• The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Cedric Cassan; Aikeranmu Kadeer; Koki Yuasa; Nozomu Sato; Kintake  Sonoike; Yasuko Kaneko; Atsuko Miyagi; Hiroko Takahashi; Toshiki Ishikawa; Masatoshi Yamaguchi; Yoshitaka Nishiyama; Yukako Hihara; Yves Gibon; Maki Kawai-Yamada
  Plant and Cell Physiology, Volume：62, Number：4, First page：668, Last page：677, Sep. 2021, [Reviewed]
  
  NADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in the unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the Photosystem II (PSII) photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain the photosynthetic capacity.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcab023
  DOI ID：10.1093/pcp/pcab023, ISSN：0032-0781, eISSN：1471-9053, PubMed ID：33210985, PubMed Central ID：PMC7781788
• Altered metabolism of chloroplastic NAD kinase-overexpressing Arabidopsis in response to magnesium sulfate supplementation
  Maki Kawai-Yamada; Atsuko Miyagi; Yuki Sato; Yuki Hosoi; Shin-Nosuke Hashida; Toshiki Ishikawa; Masatoshi Yamaguchi
  Plant Signaling & Behavior, Volume：16, Number：1, First page：1844509, Last page：1844509, Jan. 2021, [Reviewed]
  Informa UK Limited, Scientific journal
  DOI：https://doi.org/10.1080/15592324.2020.1844509
  DOI ID：10.1080/15592324.2020.1844509, eISSN：1559-2324
• An Arabidopsis NAC domain transcription factor, ATAF2 , promotes age‐dependent and dark‐induced leaf senescence　　　　　　　　　　　　　　　
  Isura Sumeda Priyadarshana Nagahage; Shingo Sakamoto; Minoru Nagano; Toshiki Ishikawa; Nobutaka Mitsuda; Maki Kawai‐Yamada; Masatoshi Yamaguchi
  Physiologia Plantarum, Volume：170, Number：2, First page：299, Last page：308, Oct. 2020, [Reviewed], [Last, Corresponding], ［International magazine］
  Leaf senescence is controlled developmentally and environmentally and is affected by numerous genes, including transcription factors. An Arabidopsis NAC domain transcription factor, ATAF2, is known to regulate biotic stress responses. Recently, we have demonstrated that ATAF2 upregulates ORE1, a key regulator of leaf senescence. Here, to investigate the function of ATAF2 in leaf senescence further, we generated and analyzed overexpressing transgenic and T-DNA inserted mutant lines. Transient expression analysis indicated that ATAF2 upregulates several NAC domain transcription factors that regulate senescence. Indeed, ATAF2 overexpression induced the expression of senescence-related genes, thereby accelerating leaf senescence, whereas the expression of such genes in ataf2 mutants was lower than that of wild-type plants. Furthermore, the ataf2 mutants exhibited significant delays in dark-induced leaf senescence. It was also found that ATAF2 induces the expression of transcription factors, which both promotes and represses leaf senescence. The present study demonstrates that ATAF2 promotes leaf senescence in response to developmental and environmental signals.
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/ppl.13156
  DOI ID：10.1111/ppl.13156, ISSN：0031-9317, eISSN：1399-3054, PubMed ID：32579231, 共同研究・競争的資金等ID：12839792;12839799
• Plant-unique cis/trans isomerism of long-chain base unsaturation is selectively required for Aluminum tolerance resulting from glucosylceramide-dependent plasma membrane fluidity　　　　　　　　　　　　　　　
  Sato M; Nagano M; Jin S; Miyagi A; Yamaguchi M; Kawai-Yamada M; Ishikawa T
  Plants, Volume：9, Number：1, First page：19, 2020, [Reviewed], [Invited], ［International magazine］
  Cis/trans isomerism of the Δ8 unsaturation of long-chain base (LCB) is found only in plant sphingolipids. This unique geometry is generated by sphingolipid LCB Δ8 desaturase SLD which produces both isomers at various ratios, resulting in diverse cis/trans ratios in plants. However, the biological significance of this isomeric diversity remains controversial. Here, we show that the plant-specific cis unsaturation of LCB selectively contributes to glucosylceramide (GlcCer)-dependent tolerance to aluminum toxicity. We established three transgenic rice lines with altered LCB unsaturation profiles. Overexpression of SLD from rice (OsSLD-OX), which preferentially exhibits cis-activity, or Arabidopsis (AtSLD-OX), showing preference for trans-activity, facilitated Δ8 unsaturation in different manners: a slight increase of cis-unsaturated glycosylinositolphosphoceramide (GIPC) in OsSLD-OX, and a drastic increase of trans-unsaturated GlcCer and GIPC in AtSLD-OX. Disruption of LCB Δ4 desaturase (des) significantly decreased the content of GlcCer. Fluorescence imaging analysis revealed that OsSLD-OX and AtSLD-OX showed increased plasma membrane fluidity, whereas des had less fluidity, demonstrating that the isomers universally contributed to increasing membrane fluidity. However, the results of a hydroponic assay showed decreased aluminum tolerance in AtSLD-OX and des compared to OsSLD-OX and the control plants, which did not correlate with membrane fluidity. These results suggest that cis-unsaturated GlcCer, not GIPC, selectively serves to maintain the membrane fluidity specifically associated with aluminum tolerance.
  English, Scientific journal
  DOI：https://doi.org/10.3390/plants9010019
  DOI ID：10.3390/plants9010019, PubMed ID：31877922, PubMed Central ID：PMC7020186
• The Class II KNOX genes KNAT3 and KNAT7 work cooperatively to influence deposition of secondary cell walls that provide mechanical support to Arabidopsis stems
  Shumin Wang; Masatoshi Yamaguchi; Etienne Grienenberger; Patrick T. Martone; A. Lacey Samuels; Shawn D. Mansfield
  The Plant Journal, Volume：101, Number：2, First page：293, Last page：309, Jan. 2020, [Reviewed]
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.14541
  DOI ID：10.1111/tpj.14541, ISSN：0960-7412, eISSN：1365-313X
• Affinity-based high-resolution analysis of DNA binding by VASCULAR-RELATED NAC-DOMAIN7 via fluorescence correlation spectroscopy　　　　　　　　　　　　　　　
  Taizo Tamura; Hitoshi Endo; Atsunobu Suzuki; Yutaka Sato; Ko Kato; Misato Ohtani; Masatoshi Yamaguchi; Taku Demura
  Plant Journal, Volume：100, Number：2, First page：298, Last page：313, Oct. 2019, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.14443
  Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85070718967&origin=inward
  Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85070718967&origin=inward
  DOI ID：10.1111/tpj.14443, ISSN：0960-7412, eISSN：1365-313X, PubMed ID：31313414, SCOPUS ID：85070718967
• One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803.　　　　　　　　　　　　　　　
  Ishikawa Y; Miyagi A; Ishikawa T; Nagano M; Yamaguchi M; Hihara Y; Kaneko Y; Kawai-Yamada M
  Plant Journal, Volume：98, Number：4, First page：654, Last page：666, May 2019, [Reviewed], ［International magazine］
  Pyridine nucleotides (NAD(P)(H)) are electron carriers that are the driving forces in various metabolic pathways. Phosphorylation of NAD(H) to NADP(H) is performed by the enzyme NAD kinase (NADK). Synechocystis sp. PCC 6803 harbors two genes (sll1415 and slr0400) that encode proteins with NADK homology. When genetic mutants for sll1415 and slr0400 (Δ1415 and Δ0400, respectively) were cultured under photoheterotrophic growth conditions only the Δ1415 cells showed a growth defect. In wild-type cells, the sll1415 transcript accumulated after the cells were transferred to photoheterotrophic conditions. Furthermore, NAD(P)(H) measurements demonstrated that a dynamic metabolic conversion was implemented during the adaptation from photoautotrophic to photoheterotrophic conditions. Electron microscopy observation and biochemistry quantification demonstrated the accumulation of glycogen in the Δ1415 cells under photoheterotrophic conditions at 96 h. Quantitative real-time reverse transcription PCR (qRT-PCR) demonstrated the accumulation of mRNAs that encoded glycogen biosynthesis-related enzymes in photoheterotrophic Δ1415 cells. At 96 h, enzyme activity measurement in the photoheterotrophic Δ1415 cells demonstrated that the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were decreased, but the activities of glucose dehydrogenase were increased. Furthermore, metabolomics analysis demonstrated that the Δ1415 cells showed increased glucose-6-phosphate and 6-phosphogluconate content at 96 h. Therefore, sll1415 has a significant function in the oxidative pentose phosphate (OPP) pathway for catabolism of glucose under photoheterotrophic conditions. Additionally, it is presumed that the slr0400 had a different role in glucose catabolism during growth. These results suggest that the two Synechocystis sp. PCC 6803 NADKs (Sll1415 and Slr0400) have distinct functions in photoheterotrophic cyanobacterial metabolism.
  English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.14262
  DOI ID：10.1111/tpj.14262, PubMed ID：30693583
• Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes.　　　　　　　　　　　　　　　
  Laddawan Saelim; Nobuhiro Akiyoshi; Tian Tian Tan; Ayumi Ihara; Masatoshi Yamaguchi; Ko Hirano; Makoto Matsuoka; Taku Demura; Misato Ohtani
  Journal of plant research, Volume：132, Number：1, First page：117, Last page：129, Jan. 2019, [Reviewed], ［Domestic magazine］
  The cell wall determines morphology and the environmental responses of plant cells. The primary cell wall (PCW) is produced during cell division and expansion, determining the cell shape and volume. After cell expansion, specific types of plant cells produce a lignified wall, known as a secondary cell wall (SCW). We functionally analyzed Group IIId Arabidopsis AP2/EREBP genes, namely ERF34, ERF35, ERF38, and ERF39, which are homologs of a rice ERF gene previously proposed to be related to SCW biosynthesis. Expression analysis revealed that these four genes are expressed in regions related to cell division and/or cell differentiation in seedlings (i.e., shoot apical meristems, the primordia of leaves and lateral roots, trichomes, and central cylinder of primary roots) and flowers (i.e., vascular tissues of floral organs and replums and/or valve margins of pistils). Overexpression of ERF genes significantly upregulated PCW-type, but not SCW-type, CESA genes encoding cellulose synthase catalytic subunits in Arabidopsis seedlings. Transient co-expression reporter analysis indicated that ERF35, ERF38, and ERF39 possess transcriptional activator activity, and that ERF34, ERF35, ERF38, and ERF39 upregulated the promoter activity of CESA1, a PCW-type CESA gene, through the DRECRTCOREAT elements, the core cis-acting elements known to be recognized by AP2/ERF proteins. Together, our findings show that Group IIId ERF genes are positive transcriptional regulators of PCW-type CESA genes in Arabidopsis and are possibly involved in modulating cellulose biosynthesis in response to developmental requirements and environmental stimuli.
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-018-1074-1
  DOI ID：10.1007/s10265-018-1074-1, PubMed ID：30478480
• An NAC domain transcription factor ATAF2 acts as transcriptional activator or repressor dependent on promoter context　　　　　　　　　　　　　　　
  Isura Sumeda Priyadarshana Nagahage; Shingo Sakamoto; Minoru Nagano; Toshiki Ishikawa; Maki Kawai-Yamada; Nobutaka Mitsuda; Masatoshi Yamaguchi
  Plant Biotechnology, Volume：35, Number：3, First page：285, Last page：289, Sep. 2018, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.18.0507a
  DOI ID：10.5511/plantbiotechnology.18.0507a
• Characterization of rice KT/HAK/KUP potassium transporters and K⁺ uptake by HAK1 from oryza sativa　　　　　　　　　　　　　　　
  Tomoyuki Okada; Sousuke Yamane; Masatoshi Yamaguchi; Ko Kato; Atsuhiko Shinmyo; Yuta Tsunemitsu; Kozo Iwasaki; Daisei Ueno; Taku Demura
  Plant Biotechnology, Volume：35, Number：2, First page：101, Last page：111, 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.18.0308a
  Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050662140&origin=inward
  Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85050662140&origin=inward
  DOI ID：10.5511/plantbiotechnology.18.0308a, ISSN：1342-4580, eISSN：1347-6114, SCOPUS ID：85050662140
• Transcription Factors VND1-VND3 Contribute to Cotyledon Xylem Vessel Formation.　　　　　　　　　　　　　　　
  Tian Tian Tan; Hitoshi Endo; Ryosuke Sano; Tetsuya Kurata; Masatoshi Yamaguchi; Misato Ohtani; Taku Demura
  Plant physiology, Volume：176, Number：1, First page：773, Last page：789, Jan. 2018, [Reviewed], ［International magazine］
  English
  DOI：https://doi.org/10.1104/pp.17.00461
  DOI ID：10.1104/pp.17.00461, ORCID：42523540, PubMed ID：29133368, PubMed Central ID：PMC5761765, Web of Science ID：WOS:000419675300060
• Evaluation of metabolic changes in oxalate-rich plant Rumex obtusifolius L. caused by ion beam irradiation.　　　　　　　　　　　　　　　
  Atsuko Miyagi; Sayaka Kitano; Yutaka Oono; Yoshihiro Hase; Issay Narumi; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  Plant physiology and biochemistry : PPB, Volume：122, First page：40, Last page：45, Jan. 2018, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.plaphy.2017.11.001
  DOI ID：10.1016/j.plaphy.2017.11.001, ORCID：42523539, PubMed ID：29172104, Web of Science ID：WOS:000423895600005
• Heterologous expression and characterization of an Arabidopsis beta-L-arabinopyranosidase and alpha-D-galactosidases acting on beta-L-arabinopyranosyl residues　　　　　　　　　　　　　　　
  Chiemi Imaizumi; Harumi Tomatsu; Kiminari Kitazawa; Yoshihisa Yoshimi; Seiji Shibano; Kaoru Kikuchi; Masatoshi Yamaguchi; Satoshi Kaneko; Yoichi Tsumuraya; Toshihisa Kotake
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：68, Number：16, First page：4651, Last page：4661, Jul. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/erx279
  DOI ID：10.1093/jxb/erx279, ISSN：0022-0957, eISSN：1460-2431, ORCID：39147297, Web of Science ID：WOS:000412100500020
• Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp PCC 6803　　　　　　　　　　　　　　　
  Yuuma Ishikawa; Atsuko Miyagi; Yuto Haishima; Toshiki Ishikawa; Minoru Nagano; Masatoshi Yamaguchi; Yukako Hihara; Maki Kawai-Yamada
  JOURNAL OF PLANT PHYSIOLOGY, Volume：205, First page：105, Last page：112, Oct. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2016.09.002
  DOI ID：10.1016/j.jplph.2016.09.002, ISSN：0176-1617, eISSN：1618-1328, ORCID：39147298, Web of Science ID：WOS:000385858600014
• The expression of a rice secondary wall-specific cellulose synthase gene, OsCesA7, is directly regulated by a rice transcription factor, OsMYB58/63　　　　　　　　　　　　　　　
  Soichiro Noda; Taichi Koshiba; Takefumi Hattori; Masatoshi Yamaguchi; Shiro Suzuki; Toshiaki Umezawa
  PLANTA, Volume：242, Number：3, First page：589, Last page：600, Sep. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-015-2343-z
  DOI ID：10.1007/s00425-015-2343-z, ISSN：0032-0935, eISSN：1432-2048, ORCID：39147303, Web of Science ID：WOS:000359831900008
• Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Isura Sumeda Priyadarshana Nagahage; Misato Ohtani; Toshiki Ishikawa; Hirofumi Uchimiya; Maki Kawai-Yamada; Taku Demura
  PLANT BIOTECHNOLOGY, Volume：32, Number：2, First page：119, Last page：U14, Jun. 2015, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.15.0208a
  DOI ID：10.5511/plantbiotechnology.15.0208a, ISSN：1342-4580, ORCID：39147299, Web of Science ID：WOS:000356970600002
• NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants　　　　　　　　　　　　　　　
  Yoshimi Nakano; Masatoshi Yamaguchiz; Hitoshi Endo; Nur Ardiyana Rejab; Misato Ohtani
  FRONTIERS IN PLANT SCIENCE, Volume：6, May 2015, [Reviewed]
  English
  DOI：https://doi.org/10.3389/fpls.2015.00288
  DOI ID：10.3389/fpls.2015.00288, ISSN：1664-462X, ORCID：39147302, Web of Science ID：WOS:000356900500001
• Multiple Classes of Transcription Factors Regulate the Expression of VASCULAR-RELATED NAC-DOMAIN7, a Master Switch of Xylem Vessel Differentiation　　　　　　　　　　　　　　　
  Hitoshi Endo; Masatoshi Yamaguchi; Taizo Tamura; Yoshimi Nakano; Nobuyuki Nishikubo; Arata Yoneda; Ko Kato; Minoru Kubo; Shinya Kajita; Yoshihiro Katayama; Misato Ohtani; Taku Demura
  PLANT AND CELL PHYSIOLOGY, Volume：56, Number：2, First page：242, Last page：254, Feb. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcu134
  DOI ID：10.1093/pcp/pcu134, ISSN：0032-0781, eISSN：1471-9053, ORCID：39147301, Web of Science ID：WOS:000351514000007
• Contribution of NAC Transcription Factors to Plant Adaptation to Land　　　　　　　　　　　　　　　
  Bo Xu; Misato Ohtani; Masatoshi Yamaguchi; Kiminori Toyooka; Mayumi Wakazaki; Mayuko Sato; Minoru Kubo; Yoshimi Nakano; Ryosuke Sano; Yuji Hiwatashi; Takashi Murata; Tetsuya Kurata; Arata Yoneda; Ko Kato; Mitsuyasu Hasebe; Taku Demura
  SCIENCE, Volume：343, Number：6178, First page：1505, Last page：1508, Mar. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1126/science.1248417
  DOI ID：10.1126/science.1248417, ISSN：0036-8075, eISSN：1095-9203, ORCID：39147304, Web of Science ID：WOS:000333471000046
• Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata　　　　　　　　　　　　　　　
  Masayuki Kakimoto; Toshiki Ishikawa; Atsuko Miyagi; Kazuaki Saito; Motonobu Miyazaki; Takashi Asaeda; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  JOURNAL OF PLANT PHYSIOLOGY, Volume：171, Number：3-4, First page：292, Last page：300, Feb. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jplph.2013.09.005
  DOI ID：10.1016/j.jplph.2013.09.005, ISSN：0176-1617, ORCID：39147305, Web of Science ID：WOS:000332052200013
• Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays　　　　　　　　　　　　　　　
  Sayaka Kitano; Atsuko Miyagi; Yutaka Oono; Yoshihiro Hase; Issay Narumi; Masatoshi Yamaguchi; Hirofumi Uchimiya; Maki Kawai-Yamada
  Metabolomics, Volume：11, Number：1, First page：134, Last page：142, 2014, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1007/s11306-014-0684-4
  Scopus：https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939895666&origin=inward
  Scopus Citedby：https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84939895666&origin=inward
  DOI ID：10.1007/s11306-014-0684-4, ISSN：1573-3882, eISSN：1573-3890, ORCID：39147300, SCOPUS ID：84939895666, Web of Science ID：WOS:000348343300013
• A Computational and Experimental Approach Reveals that the 5 '-Proximal Region of the 5 '-UTR has a Cis-Regulatory Signature Responsible for Heat Stress-Regulated mRNA Translation in Arabidopsis　　　　　　　　　　　　　　　
  Hideyuki Matsuura; Shinya Takenami; Yuki Kubo; Kiyotaka Ueda; Aiko Ueda; Masatoshi Yamaguchi; Kazumasa Hirata; Taku Demura; Shigehiko Kanaya; Ko Kato
  PLANT AND CELL PHYSIOLOGY, Volume：54, Number：4, First page：474, Last page：483, Apr. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcs189
  DOI ID：10.1093/pcp/pcs189, ISSN：0032-0781, ORCID：39147306, PubMed ID：23314753, Web of Science ID：WOS:000317611700004
• ATL54, a ubiquitin ligase gene related to secondary cell wall formation, is transcriptionally regulated by MYB46　　　　　　　　　　　　　　　
  Soichiro Noda; Masatoshi Yamaguchi; Yuta Tsurumaki; Yoshinori Takahashi; Nobuyuki Nishikubo; Takefumi Hattori; Taku Demura; Shiro Suzuki; Toshiaki Umezawa
  PLANT BIOTECHNOLOGY, Volume：30, Number：5, First page：503, Last page：U120, 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.13.0905b
  DOI ID：10.5511/plantbiotechnology.13.0905b, ISSN：1342-4580, ORCID：39147308, Web of Science ID：WOS:000330032000010
• ATL54, a RING-H2 domain protein selected by a gene co-expression network analysis, is associated with secondary cell wall formation in Arabidopsis　　　　　　　　　　　　　　　
  Soichiro Noda; Yoshinori Takahashi; Yuta Tsurumaki; Masaomi Yamamura; Nobuyuki Nishikubo; Masatoshi Yamaguchi; Nozomu Sakurai; Takefumi Hattori; Hideyuki Suzuki; Taku Demura; Daisuke Shibata; Shiro Suzuki; Toshiaki Umezawa
  PLANT BIOTECHNOLOGY, Volume：30, Number：2, First page：169, Last page：U174, 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.13.0304a
  DOI ID：10.5511/plantbiotechnology.13.0304a, ISSN：1342-4580, ORCID：39147307, Web of Science ID：WOS:000322867600009
• Genome-Wide Analyses of Changes in Translation State Caused by Elevated Temperature in Oryza sativa　　　　　　　　　　　　　　　
  Kiyotaka Ueda; Hideyuki Matsuura; Masatoshi Yamaguchi; Taku Demura; Ko Kato
  PLANT AND CELL PHYSIOLOGY, Volume：53, Number：8, First page：1481, Last page：1491, Aug. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcs092
  DOI ID：10.1093/pcp/pcs092, ISSN：0032-0781, CiNii Articles ID：40019389679, ORCID：39147309, PubMed ID：22722767, Web of Science ID：WOS:000307834200011
• High level expression of transgenes by use of 5 '-untranslated region of the Arabidopsis thaliana arabinogalactan-protein 21 gene in dicotyledons　　　　　　　　　　　　　　　
  Takeshi Matsui; Hideyuki Matsuura; Kazutoshi Sawada; Eiji Takita; Satoko Kinjo; Shinya Takenami; Kiyotaka Ueda; Naoya Nishigaki; Shotaro Yamasaki; Kensuke Hata; Masatoshi Yamaguchi; Taku Demura; Ko Kato
  PLANT BIOTECHNOLOGY, Volume：29, Number：3, First page：319, Last page：322, 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.12.0322a
  DOI ID：10.5511/plantbiotechnology.12.0322a, ISSN：1342-4580, ORCID：39147310, Web of Science ID：WOS:000307241600020
• A NAC domain protein family contributing to the regulation of wood formation in poplar　　　　　　　　　　　　　　　
  Misato Ohtani; Nobuyuki Nishikubo; Bo Xu; Masatoshi Yamaguchi; Nobutaka Mitsuda; Nadia Goue; Fusun Shi; Masaru Ohme-Takagi; Taku Demura
  PLANT JOURNAL, Volume：67, Number：3, First page：499, Last page：512, Aug. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2011.04614.x
  DOI ID：10.1111/j.1365-313X.2011.04614.x, ISSN：0960-7412, ORCID：39147311, PubMed ID：21649762, Web of Science ID：WOS:000293176600010
• VASCULAR-RELATED NAC-DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Nobutaka Mitsuda; Misato Ohtani; Masaru Ohme-Takagi; Ko Kato; Taku Demura
  Plant Journal, Volume：66, Number：4, First page：579, Last page：590, May 2011, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2011.04514.x
  DOI ID：10.1111/j.1365-313X.2011.04514.x, ISSN：0960-7412, ORCID：39147312, PubMed ID：21284754, SCOPUS ID：79955852269, Web of Science ID：WOS:000290456400013
• Identification and Analysis of transcription factors regulating expression of the master gene for xylem vessel formation　　　　　　　　　　　　　　　
  Endo Hitoshi; Yamaguchi Masatoshi; Nakano Yoshimi; Nishikubo Nobuyuki; Ohtani Misato; Kato Ko; Katayama Yoshihiro; Kajita Shinya; Demura Taku
  Plant and Cell Physiology Supplement, Volume：2011, First page：0480, Last page：0480, 2011
  We established the in vitro system for vessel element transdifferentiation in Arabidopsis Col-0 suspension cells. Through a microarray analysis of the system we identified a number of genes with drastic changes in expression during the transdifferentiation. Moreover, we revealed that VASCULAR-RELATED NAC-DOMIN7 (VND7) encoding a NAC-domain transcription factor functions as a master regulator for vessel formation. However, the regulatory mechanism of VND7 expression is still largely unknown. Hence, in this study we aimed to identify transcription factors that regulate VND7 expression. So we first selected transcription factors up-regulated at the phase that most of the cells were transdifferentiated into vessel elements. Then we performed the particle bombardment-based transient assays to evaluate whether each candidates can induce VND7 expression using VND7pro:Luciferase construct as a reporter. As a result, we found that several genes could up-regulate VND7 expression including all VND family members. So now we are further analyzing these transcription factors to reveal its function during vessel formation and try to clarify the transcriptional relationships among these factors.
  The Japanese Society of Plant Physiologists
  DOI：https://doi.org/10.14841/jspp.2011.0.0480.0
  DOI ID：10.14841/jspp.2011.0.0480.0, CiNii Articles ID：130006996826
• 植物二次細胞壁形成の転写制御機構　　　　　　　　　　　　　　　
  Masatoshi YAMAGUCHI; Misato OHTANI; Taku DEMURA
  KAGAKU TO SEIBUTSU, Volume：49, Number：11, First page：770, Last page：777, 2011, [Reviewed], [Lead]
  Masatoshi YAMAGUCHI, Misato OHTANI, Taku DEMURA, 2011, '植物二次細胞壁形成の転写制御機構', <i>KAGAKU TO SEIBUTSU</i>, vol. 49, no. 11, pp. 770-777
  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Japanese
  DOI：https://doi.org/10.1271/kagakutoseibutsu.49.770
  DOI ID：10.1271/kagakutoseibutsu.49.770, ISSN：0453-073X, CiNii Articles ID：10029873610, CiNii Books ID：AN00037573, ORCID：9105799
• A Short Period of Mannitol Stress but Not LiCl Stress Led to Global Translational Repression in Plants　　　　　　　　　　　　　　　
  Hideyuki Matsuura; Ueda Kiyotaka; Yu Ishibashi; Yuki Kubo; Masatoshi Yamaguchi; Kazumasa Hirata; Taku Demura; Ko Kato
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：74, Number：10, First page：2110, Last page：2112, Oct. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.100330
  DOI ID：10.1271/bbb.100330, ISSN：0916-8451, eISSN：1347-6947, CiNii Articles ID：10027560810, ORCID：39147313, Web of Science ID：WOS:000283624900025
• VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 Effectively Induce Transdifferentiation into Xylem Vessel Elements under Control of an Induction System　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Nadia Goue; Hisako Igarashi; Misato Ohtani; Yoshimi Nakano; Jennifer C. Mortimer; Nobuyuki Nishikubo; Minoru Kubo; Yoshihiro Katayama; Koichi Kakegawa; Paul Dupree; Taku Demura
  PLANT PHYSIOLOGY, Volume：153, Number：3, First page：906, Last page：914, Jul. 2010, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.110.154013
  DOI ID：10.1104/pp.110.154013, ISSN：0032-0889, eISSN：1532-2548, CiNii Articles ID：80021158683, ORCID：39147316, PubMed ID：20488898, Web of Science ID：WOS:000279400200002
• VND-INTERACTING2, a NAC Domain Transcription Factor, Negatively Regulates Xylem Vessel Formation in Arabidopsis　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Misato Ohtani; Nobutaka Mitsuda; Minoru Kubo; Masaru Ohme-Takagi; Hiroo Fukuda; Taku Demura
  PLANT CELL, Volume：22, Number：4, First page：1249, Last page：1263, Apr. 2010, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.108.064048
  DOI ID：10.1105/tpc.108.064048, ISSN：1040-4651, CiNii Articles ID：80021071347, ORCID：39147317, PubMed ID：20388856, Web of Science ID：WOS:000278414900022
• Transcriptional regulation of secondary wall formation controlled by NAC domain proteins　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Taku Demura
  PLANT BIOTECHNOLOGY, Volume：27, Number：3, First page：237, Last page：242, 2010, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.27.237
  DOI ID：10.5511/plantbiotechnology.27.237, ISSN：1342-4580, ORCID：39147315, Web of Science ID：WOS:000280085800004
• MYB transcription factors orchestrating the developmental program of xylem vessels in Arabidopsis roots　　　　　　　　　　　　　　　
  Yoshimi Nakano; Nobuyuki Nishikubo; Nadia Goue; Misato Ohtani; Masatoshi Yamaguchi; Yoshihiro Katayama; Taku Demura
  PLANT BIOTECHNOLOGY, Volume：27, Number：3, First page：267, Last page：272, 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.27.267
  DOI ID：10.5511/plantbiotechnology.27.267, ISSN：1342-4580, ORCID：39147314, Web of Science ID：WOS:000280085800008
• Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis　　　　　　　　　　　　　　　
  Satoshi Endo; Edouard Pesquet; Masatoshi Yamaguchi; Gen Tashiro; Mayuko Sato; Kiminori Toyooka; Nobuyuki Nishikubo; Makiko Udagawa-Motose; Minoru Kubo; Hiroo Fukuda; Taku Demura
  PLANT CELL, Volume：21, Number：4, First page：1155, Last page：1165, Apr. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.108.059154
  DOI ID：10.1105/tpc.108.059154, ISSN：1040-4651, CiNii Articles ID：80020348025, ORCID：39147318, PubMed ID：19383897, Web of Science ID：WOS:000266295800015
• An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region　　　　　　　　　　　　　　　
  Kohsuke Hashimoto; Hisako Igarashi; Shoji Mano; Chikako Takenaka; Takashi Shiina; Masatoshi Yamaguchi; Taku Demura; Mikio Nishimura; Teruo Shimmen; Etsuo Yokota
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：59, Number：13, First page：3523, Last page：3531, Oct. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/jxb/ern202
  DOI ID：10.1093/jxb/ern202, ISSN：0022-0957, eISSN：1460-2431, CiNii Articles ID：80019971620, ORCID：39147319, PubMed ID：18703495, Web of Science ID：WOS:000259973800003
• VASCULAR-RELATED NAC-DOMAIN7 is involved in the differentiation of all types of xylem vessels in Arabidopsis roots and shoots　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi; Minoru Kubo; Hiroo Fukuda; Taku Demura
  PLANT JOURNAL, Volume：55, Number：4, First page：652, Last page：664, Aug. 2008, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2008.03533.x
  DOI ID：10.1111/j.1365-313X.2008.03533.x, ISSN：0960-7412, ORCID：39147320, PubMed ID：18445131, Web of Science ID：WOS:000258287700010
• Control of cell division and transcription by cyclin-dependent kinase-activating kinases in plants　　　　　　　　　　　　　　　
  M Umeda; A Shimotohno; M Yamaguchi
  PLANT AND CELL PHYSIOLOGY, Volume：46, Number：9, First page：1437, Last page：1442, Sep. 2005, [Reviewed]
  English
  DOI：https://doi.org/10.1093/pcp/pci170
  DOI ID：10.1093/pcp/pci170, ISSN：0032-0781, CiNii Articles ID：40006875958, Web of Science ID：WOS:000232223100001
• Transcription switches for protoxylem and metaxylem vessel formation　　　　　　　　　　　　　　　
  M Kubo; M Udagawa; N Nishikubo; G Horiguchi; M Yamaguchi; J Ito; T Mimura; H Fukuda; T Demura
  GENES & DEVELOPMENT, Volume：19, Number：16, First page：1855, Last page：1860, Aug. 2005, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1101/gad.1331305
  DOI ID：10.1101/gad.1331305, ISSN：0890-9369, CiNii Articles ID：30018919280, PubMed ID：16103214, Web of Science ID：WOS:000231277600005
• Control of in vitro organogenesis by cyclin-dependent kinase activities in plants　　　　　　　　　　　　　　　
  M Yamaguchi; H Kato; S Yoshida; S Yamamura; H Uchimiya; M Umeda
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：100, Number：13, First page：8019, Last page：8023, Jun. 2003, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1332637100
  DOI ID：10.1073/pnas.1332637100, ISSN：0027-8424, CiNii Articles ID：80016025487, PubMed ID：12799469, Web of Science ID：WOS:000183845800108
• Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase　　　　　　　　　　　　　　　
  J Lee; A Das; M Yamaguchi; J Hashimoto; N Tsutsumi; H Uchimiya; M Umeda
  PLANT JOURNAL, Volume：34, Number：4, First page：417, Last page：425, May 2003, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1046/j.1365-313X.2003.01736.x
  DOI ID：10.1046/j.1365-313X.2003.01736.x, ISSN：0960-7412, PubMed ID：12753582, Web of Science ID：WOS:000182785300003
• Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana　　　　　　　　　　　　　　　
  A Shimotohno; S Matsubayashi; M Yamaguchi; H Uchimiya; M Umeda
  FEBS LETTERS, Volume：534, Number：1-3, First page：69, Last page：74, Jan. 2003, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/S0014-5793(02)03780-8
  DOI ID：10.1016/S0014-5793(02)03780-8, ISSN：0014-5793, CiNii Articles ID：80015743159, PubMed ID：12527363, Web of Science ID：WOS:000180502100012
• Activation of CDK-activating kinase is dependent on interaction with H-type cyclins in plants　　　　　　　　　　　　　　　
  M Yamaguchi; T Fabian; M Sauter; RP Bhalerao; J Schrader; G Sandberg; M Umeda; H Uchimiya
  PLANT JOURNAL, Volume：24, Number：1, First page：11, Last page：20, Oct. 2000, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1046/j.1365-313X.2000.00846.x
  DOI：https://doi.org/10.1046/j.1365-313x.2000.00846.x
  DOI ID：10.1046/j.1365-313X.2000.00846.x, ISSN：0960-7412, ORCID：39147327, Web of Science ID：WOS:000089935800002
• A rice homolog of Cdk7/MO15 phosphorylates both cyclin-dependent protein kinases and the carboxy-terminal domain of RNA polymerase II　　　　　　　　　　　　　　　
  M Yamaguchi; M Umeda; H Uchimiya
  PLANT JOURNAL, Volume：16, Number：5, First page：613, Last page：619, Dec. 1998, [Reviewed], [Lead]
  English, Scientific journal
  DOI：https://doi.org/10.1046/j.1365-313X.1998.00338.x
  DOI：https://doi.org/10.1046/j.1365-313x.1998.00338.x
  DOI ID：10.1046/j.1365-313X.1998.00338.x, ISSN：0960-7412, ORCID：39147330, Web of Science ID：WOS:000077866600010

• シロイヌナズナVND遺伝子群の遺伝学的解析
  伊藤敦也; 久保稔; 大谷美沙都; 石川寿樹; 川合真紀; 出村拓; 山口雅利
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：36th, First page：153, 15 Aug. 2018
  Japanese
  J-Global ID：201802264094945466
• イオンビームを照射した高シュウ酸植物エゾノギシギシの代謝解析
  宮城敦子; 北野沙也佳; 長谷純宏; 大野豊; 山口雅利; 川合真紀
  日本植物学会大会研究発表記録, Volume：79th, First page：134, 01 Sep. 2015
  Japanese
  J-Global ID：201502214850620962
• NAD(P)代謝改変シロイヌナズナの作出および代謝解析　　　　　　　　　　　　　　　
  鈴木渉太; 宮城敦子; 石川寿樹; 刑部敬史; 長野稔; 山口雅利; 川合真紀
  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, Volume：33rd, First page：140, 20 Jul. 2015
  Japanese
  J-Global ID：201502202919376033
• An E3 ubiquitin ligase involved in secondary wall formation　　　　　　　　　　　　　　　
  Soichiro Noda; Masatoshi Yamaguchi; Nobuyuki Nishikubo; Nozomu Sakurai; Masaomi Yamamura; Takefumi Hattori; Hideyuki Suzuki; Daisuke Shibata; Taku Demura; Shiro Suzuki; Toshiaki Umezawa
  Mar. 2015
• Functional characterization of rice MYB transcription factors involved in secondary cell wall biosynthesis　　　　　　　　　　　　　　　
  Noda, S; Koshiba, T; Yamaguchi, M; Hattori, T; Suzuki, S; Umezawa, T
  XXVIIth International Conference on Polyphenols, Sep. 2014, [Invited]
• 道管細胞と繊維細胞を作り分けるシス配列-転写因子結合制御の分子基盤の解明　　　　　　　　　　　　　　　
  田村泰造; 山口雅利; 遠藤仁; 米田新; 久保稔; 加藤晃; 大谷美沙都; 出村拓; 出村拓
  Volume：37th, 2014
  J-Global ID：201502208023422025
• 道管細胞分化マスター制御因子VND7はタイトにその発現が制御される　　　　　　　　　　　　　　　
  遠藤仁; 山口雅利; TAN Tian Tian; 田村泰造; 久保稔; 大谷美沙都; 出村拓; 出村拓
  Volume：37th, 2014
  J-Global ID：201502260216732643
• 道管細胞分化におけるタンパク質S-ニトロシル化修飾の役割の解明　　　　　　　　　　　　　　　
  川邊陽文; 大谷美沙都; 山口雅利; 久保稔; 倉田哲也; 坂本智昭; 米田新; 加藤晃; 出村拓; 出村拓
  Volume：78th, 2014
  J-Global ID：201402276448409810
• ガンマ線が高シュウ酸植物の代謝に及ぼす影響
  北野沙也佳; 宮城敦子; 大野豊; 長谷純弘; 鳴海一成; 山口雅利; 内宮博文; 川合真紀
  日本植物学会大会研究発表記録, Volume：77th, First page：185, 20 Aug. 2013
  Japanese
  J-Global ID：201302255221317192
• VND7による道管要素分化誘導系を用いたキシランキシロシルトランスフェラーゼの生化学的解析　　　　　　　　　　　　　　　
  深水祐一郎; 中野仁美; 山口雅利; 石水毅; 出村拓; 出村拓
  Volume：53rd, 2012
  J-Global ID：201202213414100741
• タバコBY-2細胞を用いた道管要素分化誘導システムの構築-二次壁形成における分子機構の解明に向けて-　　　　　　　　　　　　　　　
  中野仁美; 深水祐一郎; 伊藤由希子; グエ ナディア; 五十嵐久子; 山口雅利; 石水毅; 出村拓; 出村拓
  Volume：53rd, 2012
  J-Global ID：201202283424277095
• 道管分化マスター因子の発現制御機構の解明　　　　　　　　　　　　　　　
  遠藤仁; 山口雅利; 中野仁美; 米田新; 加藤晃; 出村拓
  Volume：30th, 2012
  J-Global ID：201302254240186272
• 二次細胞壁形成の時空間的制御 (特集 植物細胞壁研究の新局面 : サステナブル社会構築の基盤となる循環型炭素資源)　　　　　　　　　　　　　　　
  大谷 美沙都; 山口 雅利; 出村 拓
  Volume：66, Number：1, First page：40, Last page：46, Jan. 2012
  Japanese
  ISSN：0387-0022, CiNii Articles ID：40019142214, CiNii Books ID：AN00015468
• 道管分化マスター因子の発現を制御する転写因子の探索とその解析　　　　　　　　　　　　　　　
  遠藤仁; 遠藤仁; 山口雅利; 中野仁美; 西窪伸之; 大谷美沙都; 加藤晃; 片山義博; 梶田真也; 出村拓
  Volume：52nd, 2011
  J-Global ID：201102292421319805
• 非モデル樹木ヤトロファの次世代トランスクリプトーム解析　　　　　　　　　　　　　　　
  中野仁美; 坂本智昭; 大谷美沙都; 山口雅利; 倉田哲也; 出村拓; 出村拓
  Volume：75th, 2011
  J-Global ID：201102231679331797
• 植物細胞壁形成を司る遺伝子発現制御機構　　　　　　　　　　　　　　　
  山口雅利; 大谷美沙都; 中野仁美; 出村拓; 出村拓
  Volume：75th, 2011
  J-Global ID：201102236607613137
• 二次細胞壁形成の生化学的解析　　　　　　　　　　　　　　　
  山口雅利; 中野仁美; 武藤裕貴; 伊藤由希子; 深水祐一郎; 加藤晃; 石水毅; 出村拓; 出村拓
  2011
  ISSN：0037-1017, J-Global ID：201202226282928090
• 二次壁形成に関わるRING fingerタンパク質の局在と転写因子による発現制御　　　　　　　　　　　　　　　
  野田壮一郎; 鈴木史朗; 山口雅利; 服部武文; 西窪伸之; 出村拓; 梅澤俊明
  2011
• 道管分化マスター因子の発現を制御する転写因子の探索とその解析　　　　　　　　　　　　　　　
  遠藤仁; 遠藤仁; 山口雅利; 中野仁美; 西窪伸之; 大谷美沙都; 片山義博; 出村拓; 出村拓
  Volume：51st, 2010
  J-Global ID：201002286014242795
• 転写因子を用いた道管分化誘導システム　　　　　　　　　　　　　　　
  山口雅利; 山口雅利; NADIA Goue; 五十嵐久子; 大谷美沙都; 大谷美沙都; 中野仁美; 中野仁美; MORTIMER Jennifer C; 西窪伸之; 久保稔; 片山義博; 掛川弘一; DUPREE Paul; 出村拓; 出村拓; 出村拓
  Volume：28th, 2010
  J-Global ID：201102284406482470
• シロイヌナズナの二次壁形成に関与するRING fingerタンパク質の機能解析　　　　　　　　　　　　　　　
  野田壮一郎; 鈴木史朗; 西窪伸之; 山口雅利; 出村拓; 服部武文; 梅澤俊明
  2010
• 木化初期に発現するRING fingerタンパク質の機能解析　　　　　　　　　　　　　　　
  野田壮一郎; 鈴木史朗; 西窪伸之; 山口雅利; 出村拓; 服部武文; 梅澤俊明
  2010
• 機能未知遺伝子TED6・TED7は道管細胞壁形成の新規コンポーネントである　　　　　　　　　　　　　　　
  遠藤暁詩; 遠藤暁詩; 遠藤暁詩; PESQUET Edouard; 山口雅利; 田代玄; 佐藤繭子; 豊岡公徳; 西窪伸之; 本瀬真樹子; 久保稔; 福田裕穂; 福田裕穂; 出村拓
  Volume：73rd, 2009
  J-Global ID：200902280934707308
• 転写因子の活性化による効率的な道管要素分化誘導システムの開発　　　　　　　　　　　　　　　
  山口雅利; 五十嵐久子; 大谷美沙都; GOUE Nadia; 中野仁美; 中野仁美; 西窪伸之; 出村拓
  Volume：49th, 2008
  J-Global ID：200902256767883338
• FUNCTIONAL ANALYSIS OF NAC-DOMAIN PROTEINS THAT REGULATE XYLEM VESSEL DIFFERENTIATION　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; KUBO Minoru; FUKUDA Hiroo; DEMURA Taku
  Volume：119, First page：164, Last page：164, 01 Dec. 2006
  English
  ISSN：0918-9440, CiNii Articles ID：10019309878, CiNii Books ID：AA10900218
• 高等植物における道管形成のマスター制御因子であるVND6とVND7
  久保稔; 宇田川真理子; 西窪伸之; 堀口吾朗; 山口雅利; 井藤純; 三村徹郎; 福田裕穂; 出村拓
  日本分子生物学会年会講演要旨集, Volume：28th, First page：718, 25 Nov. 2005
  Japanese
  J-Global ID：200902242102699632
• Control of cell division and transcription by cyclin-dependent kinase-activating kinases in plants　　　　　　　　　　　　　　　
  M Umeda; A Shimotohno; M Yamaguchi
  PLANT AND CELL PHYSIOLOGY, Volume：46, Number：9, First page：1437, Last page：1442, Sep. 2005
  English, Book review
  DOI：https://doi.org/10.1093/pcp/pci170
  DOI ID：10.1093/pcp/pci170, ISSN：0032-0781, Web of Science ID：WOS:000232223100001
• Transcription switches for protoxylem and metaxylem vessel formation　　　　　　　　　　　　　　　
  M Kubo; M Udagawa; N Nishikubo; G Horiguchi; M Yamaguchi; J Ito; T Mimura; H Fukuda; T Demura
  GENES & DEVELOPMENT, Volume：19, Number：16, First page：1855, Last page：1860, Aug. 2005
  English
  DOI：https://doi.org/10.1101/gad.1331305
  DOI ID：10.1101/gad.1331305, ISSN：0890-9369, Web of Science ID：WOS:000231277600005
• Characterication of novel NAC-domain proteins interacting with VND7　　　　　　　　　　　　　　　
  M Yamaguchi; M Kubo; H Fukuda; T Demura
  PLANT AND CELL PHYSIOLOGY, Volume：46, First page：S196, Last page：S196, 2005
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000228104101288
• Functional analyses of VND genes of Arabidopsis for vascular development　　　　　　　　　　　　　　　
  M Kubo; M Yamaguchi; H Fukuda; T Demura
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S61, Last page：S61, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700240
• Cell cycle function of a rice B2-type cyclin interacting with a B-type cyclin-dependent kinase　　　　　　　　　　　　　　　
  J Lee; A Das; M Yamaguchi; J Hashimoto; N Tsutsumi; H Uchimiya; M Umeda
  PLANT JOURNAL, Volume：34, Number：4, First page：417, Last page：425, May 2003
  English
  DOI：https://doi.org/10.1046/j.1365-313X.2003.01736.x
  DOI ID：10.1046/j.1365-313X.2003.01736.x, ISSN：0960-7412, Web of Science ID：WOS:000182785300003
• Differential phosphorylation activities of CDK-activating kinases in Arabidopsis thaliana　　　　　　　　　　　　　　　
  A Shimotohno; S Matsubayashi; M Yamaguchi; H Uchimiya; M Umeda
  FEBS LETTERS, Volume：534, Number：1-3, First page：69, Last page：74, Jan. 2003
  English
  DOI：https://doi.org/10.1016/S0014-5793(02)03780-8
  DOI ID：10.1016/S0014-5793(02)03780-8, ISSN：0014-5793, Web of Science ID：WOS:000180502100012
• Analysis of transgenic tobacco plants expressing rice CDK-activating kinase (CAK)　　　　　　　　　　　　　　　
  M Yamaguchi; S Yamamura; M Umeda; H Uchimiya
  PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S213, Last page：S213, 2002
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000174726400752
• Ara6, a plant-unique novel type Rab GTPase, functions in the endocytic pathway of Arabidopsis thaliana　　　　　　　　　　　　　　　
  T Ueda; M Yamaguchi; H Uchimiya; A Nakano
  EMBO JOURNAL, Volume：20, Number：17, First page：4730, Last page：4741, Sep. 2001
  English
  DOI：https://doi.org/10.1093/emboj/20.17.4730
  DOI ID：10.1093/emboj/20.17.4730, ISSN：0261-4189, Web of Science ID：WOS:000170907900012
• ANALYSIS OF THE REGULATORY MECHANISM ON CDK-ACTVATING KINASE (CAK) :　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; YAMAMURA Saburo; UMEDA Masaaki; UCHIMIYA Hirofumi
  Plant and cell physiology, Volume：42, First page：s46, 2001
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003715320, CiNii Books ID：AA0077511X
• MECHANISM OF ENDOSOMAL LOCALIZATION AND GTPase CYCLE REGULATION OF Ara6,A NOVEL PLANT-SPECIFIC Rab/Ypt GTPase :　　　　　　　　　　　　　　　
  UEDA Takashi; YAMAGUCHI Masatoshi; UCHIMIYA Hirofumi; NAKANO Akihiko
  Plant and cell physiology, Volume：42, First page：s219, 2001
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003715997, CiNii Books ID：AA0077511X
• Molecular dissection of cell death in rice (共著)　　　　　　　　　　　　　　　
  IRRI: Rice Genetics Ⅳ, Volume：pp.365-376, 2001
• CYCLIN H INTERACTS WITH AND ACTIVATES CDK-ACTIVATING KINASE IN RICE :　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; UMEDA Masaaki; UCHIMIYA Hirofumi
  Plant and cell physiology, Volume：41, First page：s134, 2000
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003722413, CiNii Books ID：AA0077511X
• Ara6,A NOVEL PLANT-SPECIFIC Rab/Ypt GTPase, RESIDES ON ENDOCYTIC MEMBRANES :　　　　　　　　　　　　　　　
  UEDA Takashi; YAMAGUCHI Masatoshi; UCHIMIYA Hirofumi; NAKANO Akihiko
  Plant and cell physiology, Volume：41, First page：s31, 2000
  Japanese Society of Plant Physiologists, English
  ISSN：0032-0781, CiNii Articles ID：110003722056, CiNii Books ID：AA0077511X
• Molecular characterization of mitotic cyclins in rice plants　　　　　　　　　　　　　　　
  M Umeda; N Iwamoto; C Umeda-Hara; M Yamaguchi; J Hashimoto; H Uchimiya
  MOLECULAR AND GENERAL GENETICS, Volume：262, Number：2, First page：230, Last page：238, Sep. 1999
  English
  DOI：https://doi.org/10.1007/s004380051079
  DOI ID：10.1007/s004380051079, ISSN：0026-8925, CiNii Articles ID：30009502304, PubMed ID：10517318, Web of Science ID：WOS:000082852000004
• CHARACTERIZATION OF FACTORS INTERACTING WITH CDK-ACTIVATING KINASE OF RICE　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; UMEDA Masaaki; HASHIMOTO Junji; UCHIMIYA Hirofumi
  Volume：40, First page：s162, Last page：s162, Mar. 1999
  English
  ISSN：0032-0781, CiNii Articles ID：10003758381, CiNii Books ID：AA0077511X
• Differential expression of genes for cyclin-dependent protein kinases in rice plants　　　　　　　　　　　　　　　
  M Umeda; C Umeda-Hara; M Yamaguchi; J Hashimoto; H Uchimiya
  PLANT PHYSIOLOGY, Volume：119, Number：1, First page：31, Last page：40, Jan. 1999
  English
  DOI：https://doi.org/10.1104/pp.119.1.31
  DOI ID：10.1104/pp.119.1.31, ISSN：0032-0889, CiNii Articles ID：30019360023, PubMed ID：9880343, Web of Science ID：WOS:000078048300005
• Functional analysis of rice CDK-activating kinase　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; UMEDA Masaaki; HASHIMOTO Junji; UCHIMIYA Hirofumi
  Volume：21, First page：507, Last page：507, 01 Dec. 1998
  Japanese
  CiNii Articles ID：10002919420, CiNii Books ID：AN10468414
• FUNCTIONAL ANALYSIS OF RICE CYCLIN-DEPENDENT KINASES　　　　　　　　　　　　　　　
  YAMAGUCHI Masatoshi; UMEDA Masaaki; UMEDA-HARA Chikage; HASHIMOTO Junji; UCHIMIYA Hirofumi
  Volume：39, First page：S53, Last page：S53, May 1998
  English
  ISSN：0032-0781, CiNii Articles ID：10003751680, CiNii Books ID：AA0077511X
• DIFFERENTIAL EXPRESSION OF cdc2-RELATED GENES IN RICE　　　　　　　　　　　　　　　
  UMEDA Masaaki; UMEDA-HARA Chikage; YAMAGUCHI Masatoshi; HASHIMOTO Junji; UCHIMIYA Hirofumi
  Volume：38, First page：s81, Mar. 1997
  English
  ISSN：0032-0781, CiNii Articles ID：10004345700, CiNii Books ID：AA0077511X
• ANALYSIS OF AERENCHYMA FORMATION CAUSED BY CELL DEATH IN RICE ROOT　　　　　　　　　　　　　　　
  KAWAI Maki; SAMARAJEEWA P. K.; YAMAGUCHI Masatoshi; UCHIMIYA Hirofumi
  Volume：37, First page：131, Last page：131, Mar. 1996
  English
  ISSN：0032-0781, CiNii Articles ID：10002708306, CiNii Books ID：AA0077511X
• RING fingerタンパク質ATL54の機能解析　　　　　　　　　　　　　　　
  野田壮一郎; 鈴木史朗; 山口雅利; 西窪伸之; 櫻井望; 服部武文; 鈴木秀幸; 出村拓; 柴田大輔; 梅澤俊明

• 二次細胞壁マスター因子の転写制御機構　　　　　　　　　　　　　　　
  向井陸馬; 清水悠裕; 藤澤りみり; 満山進; 坂本真吾; 光田展隆; 石川寿樹; 川合真紀; 山口雅利
  Sep. 2024
  Aug. 2024 - Sep. 2024, Japanese, Oral presentation
  共同研究・競争的資金等ID：48984418;48984419
• Master regulators of formation of secondary cell wall, main target of the woody biomass　　　　　　　　　　　　　　　
  Masatoshi Yamaguchi
  Genomic Big Bang-Evolution, Biodiversity, and Sustainable Bio-inovation-, May 2024, [Invited]
  May 2024 - May 2024, English, Invited oral presentation
  共同研究・競争的資金等ID：48984418;48984419

• 2025 - Present
  バイオテクノロジー特論, Postgraduate courses
• 2025 - Present
  生命化学, Undergraduate special subjects
• 2016 - Present
  生物学基礎, Undergraduate special subjects
• 2016 - Present
  生物代謝工学特論, Postgraduate courses
• 2018 - 2021
  分子細胞学, Undergraduate special subjects
• 2012 - 2021
  生物代謝工学, Postgraduate courses
• 2014 - 2020
  バイオテクノロジー
• 2012 - 2020
  環境共生学実験
• 2017 - 2017
  生物学特講, Undergraduate special subjects
• 2013 - 2014
  生物学序説

• Canadian Society of Plant Biologist

• 二次細胞壁を形成する道管要素と繊維細胞分化制御機構の解明　　　　　　　　　　　　　　　
  01 Apr. 2024 - 31 Mar. 2027
  Grant amount(Total)：4680000, Direct funding：3600000, Indirect funding：1080000
  Grant number：24K08823
  講演・口頭発表等ID：50178821
• Molecular mechanism for synthesis of acetylated glucomannan　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2023 - 31 Mar. 2027
  Saitama University
  Grant amount(Total)：18720000, Direct funding：14400000, Indirect funding：4320000
  Grant number：23K26827
  講演・口頭発表等ID：50178821
• Multidimensional Instantaneous and noninvasive monitoring of environmental stress and activities of plant using highly sensitive interferometric techniques　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2019 - 31 Mar. 2022
  Saitama University
  Grant amount(Total)：17420000, Direct funding：13400000, Indirect funding：4020000
  Grant number：19H04289
• Biological roles of VNI2 forming complexes with various NAC domain transcription factors　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Apr. 2018 - Mar. 2021
  Yamaguchi Masatoshi, Saitama University, Principal investigator
  A NAC domain transcription factor, VNI2, is isolated as an interacting factor with another NAC domain transcription factor, VND7, which is a key regulator of xylem vessel formation. In addition, we have found that VNI2 also interacts with a number of NAC domain transcription factors. Here, we tried to understand biological meaning of interaction of VNI2 with various types of NAC domain transcription factors. It was found that VNI2 plays roles in leaf senescence and phloem differentiation by forming distinct NAC domain transcription factors. It was also found that a conserved amino acid region of VNI2 confers effective inhibition of VND7 function.
  Competitive research funding, Grant number：18K06277
  論文ID：31422729
• カビ臭産出関連遺伝子の分析を用いた次世代型貯水池カビ臭対策技術の開発　　　　　　　　　　　　　　　
  Apr. 2015 - Mar. 2018
  Competitive research funding
• 単一種のマングローブの植林による生態系と景観の改変の実態把握と改善法の開発　　　　　　　　　　　　　　　
  Apr. 2015 - Mar. 2018
  Competitive research funding
• NACドメイン転写因子へテロ複合体の生物学的役割　　　　　　　　　　　　　　　
  Apr. 2015 - Mar. 2018
  Principal investigator
  Competitive research funding
  論文ID：31422729
• Comprehensive omics analysis of plant cell wall formation　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 28 Jun. 2012 - 31 Mar. 2017
  DEMURA Taku; YONEDA Arata; YAMAGUCHI Masatoshi; NARAMOTO Satoshi, Nara Institute of Science and Technology
  Grant amount(Total)：111670000, Direct funding：85900000, Indirect funding：25770000
  We performed a series of omics analyses on plant cell wall formation. Transcriptome/metabolome analysis revealed that the primary metabolism is strongly activated during secondary cell wall formation. Also, proteome analysis showed that the amount of several enzymes related to lignin biosynthesis and pectin degradation is drastically changed during secondary cell wall formation. On the analysis of primary cell wall formation, we established a system in which primary cell wall regeneration is induced from protoplasts of Arabidopsis cultured cells and mesophyll cells, using which we showed that KORRIGAN2 protein is closely associated with primary cell wall regeneration through transcriptome analysis and mutant analysis. Furthermore, we succeeded in indicating that in a moss plant, Physcomitrella patens, differentiation of water conducting cells (hydrioids) and supporting cells (stereids) is positively controlled by VNS genes, P. patens homologues of VND7.
  Grant number：24114002
• 転写抑制因子を活用したリグノセルロース点含有植物の作出　　　　　　　　　　　　　　　
  Dec. 2011 - Mar. 2015
  Principal investigator
  Grant amount(Total)：52000000, Direct funding：40000000, Indirect funding：12000000
  Competitive research funding
• Study on protein complexes of NAC transcription factor family　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), 2013 - 2014
  YAMAGUCHI Masatoshi, Saitama University, Principal investigator
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  NAC transcription factor family has been known to be involved in various biological regulations during plant development. Previously, we isolated NAC transcription factors, VNI1 and VNI2, as interacting factors with another NAC transcription factor VND7, a key regulator of xylem vessel formation. Here, we demonstrated that NAC domain transcription factors VNI1 and its closest homolog, ANAC103, are able to form protein complexes with several NAC domain transcription factors. In addition, by using a cDNA library for yeast two-hybrid screening containing full-length transcription factors, we succeeded in isolation of 30 members of NAC transcription factors as interacting factors with VNI2. Some of them are known to be involved in stress responses or senescence, suggesting that protein complexes of NAC transcription factor may play pivotal roles in various biological events as well as xylem vessel formation.
  Competitive research funding, Grant number：25840098
• Elucidation of regulatory system for secondary cell wall formation　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 2010 - 2012
  DEMURA Taku; YAMAGUCHI Masatoshi, Nara Institute of Science and Technology
  Grant amount(Total)：18980000, Direct funding：14600000, Indirect funding：4380000
  Secondary cell walls (SCWs) of plants are important because they are major components of woody biomass. However, little is known about mechanisms underlying SCW formation. In this study, we revealed several new aspects of the mechanisms through the analysis of biosynthesis of cellulose and xylan, major constituents of SCWs, and the analysis of regulatory system of VND7, the master regulatory switch of SCW formation, by using a new in vitro SCW induction experimental system that was established with the VND7 gene.
  Grant number：22370020
• Analysis of downstream genes regulated by a master gene of xylem vessel differentiation　　　　　　　　　　　　　　　
  Ministry of Education, Culture, Sports, Science and Technology, Grants-in-Aid for Scientific Research(若手研究(B)), 若手研究(B), 2008 - 2009
  Masatoshi YAMAGUCHI, 独立行政法人理化学研究所->奈良先端科学技術大学院大学, Principal investigator
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  In order to understand a molecular mechanism underlying xylem vessel differentiation, I characterized VASCULAR-RELATED NAC-DOMAIN7 (VND7) which plays a master regulator of xylem vessel differentiation.
  Competitive research funding, Grant number：20770041
• 維管束メリステムからの細胞運命決定機構　　　　　　　　　　　　　　　
  2008 - 2008
  Grant amount(Total)：3000000, Direct funding：3000000
  Grant number：20061029
• 維管束分化を制御するタンパク質-タンパク質相互作用因子の探索と作用機構の解析　　　　　　　　　　　　　　　
  2006 - 2007
  Principal investigator
  Grant amount(Total)：3700000, Direct funding：3700000
  Competitive research funding, Grant number：18770045
• 植物における細胞の分裂と分化を制御する分子機構の解析　　　　　　　　　　　　　　　
  2004 - 2005
  Principal investigator
  Grant amount(Total)：3700000, Direct funding：3700000
  Competitive research funding, Grant number：16770047
• 植物細胞に特徴的な分裂期を制御する細胞周期関連因子に関する解析　　　　　　　　　　　　　　　
  2000 - 2001
  Grant amount(Total)：2000000, Direct funding：2000000
  Grant number：00J08639

• 転写抑制因子を利用した植物の二次細胞壁の量的形質を改変する方法　　　　　　　　　　　　　　　
  Patent right