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SAITO Shingo
| Material Science Division | Professor |
| Department of Applied Chemistry |
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Researcher information
■ Degree■ Research Keyword
- gel electrophoresis
- fluorescence probe
- humic substances
- Actinide
- Metalloprotein
- DNA aptamer
- Kinetics
- Metal complex
- Capillary electrophoresis
- HPLC
- Fluorescent probe
- Capillary electrophresis
■ Career
- Apr. 2024 - Present, Rikkyo University
- Mar. 2017 - Present, Saitama University, Graduate School of Science and Technology, Professor
- Apr. 2007 - Mar. 2017, Saitama University, Graduate School of Science and Engineering
- Sep. 2010 - Aug. 2011, Wake Forest University
- 2001 - Mar. 2007, Kitami Institute of Technology, Faculty of Engineering
- 2001, Tohoku University, Japan
- 1998, Tohoku University, Japan
- 1996, Tohoku University, Faculty of Engineering, Japan
- Apr. 2025 - Present
The Japan Society for Analytical Chemistry, Vice Editor in Chief, Analytical Sciences, Society - Apr. 2023 - Present
- 2022 - Present
- Apr. 2021 - Present
- 2018 - Present
- 2015 - Present
- 2019 - 2023
- 2017 - 2022
- 2019
- 2017
- 2013 - 2015
- 2015
- 2009 - 2011
- 2004 - 2006
- 2005
- 2004
- Feb. 2024, Hot Article Award, Holo/apo conversion two-dimensional urea PAGE for speciation of Fe3+-bound transferrin in serum, Analytical Sciences
Shingo Saito;Junko Ishikawa;Moe Ono;Yuiko Tasaki-Handa;Masami Shibukawa - Nov. 2023, 寺部賞
- Jul. 2013, 堀場賞 特別賞
- Jul. 2013, 先行基礎工学研究 表彰
- Jun. 2011, Poster award, Highly Emissive Metal Probes Suitable for Ultratrace Detection of Lanthanide and Actinide Ions by Capillary Electrophoresis-Laser-Induced Fluorescence, 36th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC2011)
SAITO Shingo - 2008, 日本分析化学会奨励賞
Japan - Feb. 2005, 2004年度北海道分析化学奨励賞
Japan
Performance information
■ Paper- Reliable Post-SELEX Design of Promising Bivalent Aptamers through the Systematic Capillary Electrophoresis Exploration of Ternary DNA–Protein Complexes
Yota Suzuki; Hirochika Tsunehara; Saori Miyauchi; Masato Miyakawa; Kai Sugiyama; Masahiko Ishimitsu; Yuiko Tasaki-Handa; Keitaro Yoshimoto; Shingo Saito
Analytical Chemistry, Volume：97, Number：21, First page：10978, Last page：10987, May 2025, [Reviewed], [Last, Corresponding]
American Chemical Society (ACS), Scientific journal
DOI：https://doi.org/10.1021/acs.analchem.4c04825
DOI ID：10.1021/acs.analchem.4c04825, ISSN：0003-2700, eISSN：1520-6882 - キャピラリー電気泳動1 ラウンド選抜法によるDNA アプタマー配列データのAI解析
齋藤伸吾
Volume：11, First page：404, Last page：412, Nov. 2024, [Reviewed], [Lead, Corresponding] - Cooperativity between coordinative unsaturated Fe(
iii ) and aryl-π electrons in MIL-100(Fe) for adsorption of small molecules
Yuiko Tasaki-Handa; Kazuki Shibuya; Shingo Saito
New Journal of Chemistry, Volume：48, Number：24, First page：11107, Last page：11113, May 2024, [Reviewed]
Coordinatively unsaturated sites and aryl-π electrons coorperatively affect the adsorption of aromatics with polar groups.
Royal Society of Chemistry (RSC), Scientific journal
DOI：https://doi.org/10.1039/d4nj01385a
DOI ID：10.1039/d4nj01385a, ISSN：1144-0546, eISSN：1369-9261 - Multistep pH-peak-focusing liquid chromatography with a hydrophilic polymer gel column for separation of rare earth elements
Masami Shibukawa; Yosuke Onoyama; Yuiko Handa-Tasaki; Shingo Saito
Journal of Chromatography A, Volume：1721, First page：464829, Last page：464829, Apr. 2024, [Reviewed]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2024.464829
DOI ID：10.1016/j.chroma.2024.464829, ISSN：0021-9673 - Determination of 90Sr in highly radioactive aqueous samples via conversion to a kinetically stable 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex followed by concentration–separation–fractionation based on capillary electrophoresis–liquid scintillation
Kazuki Ouchi; Tomoko Haraga; Kazuki Hirose; Yuika Kurosawa; Yoshiyuki Sato; Masami Shibukawa; Shingo Saito
Analytica Chimica Acta, Volume：1298, First page：342399, Last page：342399, Apr. 2024, [Reviewed], [Last, Corresponding]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.aca.2024.342399
DOI ID：10.1016/j.aca.2024.342399, ISSN：0003-2670 - Structure of polyethylene glycol/water mixtures as a separation medium of aqueous two-phase extraction systems studied by equilibrium analysis of thiocyanato complexation of Cobalt(II)
Masami Shibukawa; Tomoyasu Hirasawa; Shingo Saito
Polymer, First page：126658, Last page：126658, Jan. 2024, [Reviewed]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.polymer.2023.126658
DOI ID：10.1016/j.polymer.2023.126658, ISSN：0032-3861 - Holo/apo conversion two-dimensional urea PAGE for speciation of Fe3+-bound transferrin in serum
Shingo Saito; Junko Ishikawa; Moe Ono; Yuiko Tasaki-Handa; Masami Shibukawa
Analytical Sciences, Nov. 2023, [Reviewed], [Lead, Corresponding]
Springer Science and Business Media LLC, Scientific journal
DOI：https://doi.org/10.1007/s44211-023-00460-z
DOI ID：10.1007/s44211-023-00460-z, ISSN：0910-6340, eISSN：1348-2246 - Capillary Electrophoresisi
Shingo Saito
Number：10, First page：390, Last page：397, Oct. 2023, [Reviewed], [Invited], [Lead, Corresponding] - Americium(III)/Curium(III) Complete Separation and Sensitive Fluorescence Detection by Capillary and Gel Electrophoresis Using Emissive Hexadentate/Octadentate Polyaminocarboxylate Ligands
Shingo Saito; Tomoko Haraga; Kazuki Marumo; Yoshiyuki Sato; Yuta Nakano; Yuiko Tasaki-Handa; Masami Shibukawa
Bulletin of the Chemical Society of Japan, Volume：96, Number：3, First page：223, Last page：225, Mar. 2023, [Reviewed], [Lead, Corresponding]
The Chemical Society of Japan, Scientific journal
DOI：https://doi.org/10.1246/bcsj.20220346
DOI ID：10.1246/bcsj.20220346, ISSN：0009-2673, eISSN：1348-0634 - Unusually Kinetically Inert Monocationic Neptunyl Complex with a Fluorescein-Modified 1,10-Phenanthroline-2,9-dicarboxylate Ligand: Specific Separation and Detection in Gel Electrophoresis
Kazuhito Yamagata; Kazuki Ouchi; Kazuki Marumo; Yuiko Tasaki-Handa; Tomoko Haraga; Shingo Saito
Inorganic Chemistry, Volume：62, Number：2, First page：730, Last page：738, Jan. 2023, [Reviewed], [Last, Corresponding]
American Chemical Society (ACS), Scientific journal
DOI：https://doi.org/10.1021/acs.inorgchem.2c02908
DOI ID：10.1021/acs.inorgchem.2c02908, ISSN：0020-1669, eISSN：1520-510X - Lanthanide ion exchange modulated via crystalline phase transition of a mixed-metal coordination polymer based on bis(4-nitrophenyl) phosphate
Yuiko Tasaki-Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
Inorganic Chemistry Communications, Volume：140, First page：109428, Last page：109428, Jun. 2022, [Reviewed], [Last]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.inoche.2022.109428
DOI ID：10.1016/j.inoche.2022.109428, ISSN：1387-7003 - Characterization of the Interfacial Liquid Layer Formed on Hydrophobic Packing Material Surfaces by Liquid Chromatographic Analysis of the Distribution of Ions and Molecules
Masami Shibukawa; Hiroki Okutsu; Shingo Saito
ACS Omega, Apr. 2022, [Reviewed]
American Chemical Society (ACS), Scientific journal
DOI：https://doi.org/10.1021/acsomega.2c01184
DOI ID：10.1021/acsomega.2c01184, ISSN：2470-1343, eISSN：2470-1343 - Separation approaches towards understanding supramolecular aggregate formation of humic acid
Shingo Saito
Analytical Sciences, Volume：38, Number：2, First page：233, Last page：234, Feb. 2022, [Reviewed], [Lead, Last, Corresponding]
Springer Science and Business Media LLC, Scientific journal
DOI：https://doi.org/10.1007/s44211-021-00020-3
DOI ID：10.1007/s44211-021-00020-3, ISSN：0910-6340, eISSN：1348-2246 - A chromatographic approach for studying the adsorption of polar small molecules on tetrabutylammonium bromide semiclathrate hydrate
Yuiko Tasaki-Handa; Atsuto Iwashita; Masami Shibukawa; Shingo Saito
Analytical Sciences, Volume：38, Number：1, First page：85, Last page：90, Jan. 2022, [Reviewed], [Last]
Springer Science and Business Media LLC, Scientific journal
DOI：https://doi.org/10.2116/analsci.21p182
DOI ID：10.2116/analsci.21p182, ISSN：0910-6340, eISSN：1348-2246 - Quantitation of Trace Lanthanide and Actinide Ions in Radioactive Samples by Capillary Electrophoresis-Laser-Induced Fluorescence Detection
Tomoko HARAGA; Shingo SAITO
BUNSEKI KAGAKU, Volume：70, Number：12, First page：671, Last page：679, Dec. 2021, [Reviewed], [Invited], [Last, Corresponding]
Japan Society for Analytical Chemistry, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.70.671
DOI ID：10.2116/bunsekikagaku.70.671, ISSN：0525-1931 - Alkali Metal Ion-exchange in a Metal-Organic Framework Based on Lanthanum and 1,4-Phenylenebis(methylidyne)tetrakis(phosphonic acid)
Yuiko Tasaki-Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
Analytical Sciences, Volume：37, Number：12, First page：1835, Last page：1837, Dec. 2021, [Reviewed], [Last]
Springer Science and Business Media LLC, Scientific journal
DOI：https://doi.org/10.2116/analsci.21n022
DOI ID：10.2116/analsci.21n022, ISSN：0910-6340, eISSN：1348-2246 - Stoichiometry between Humate Unit Molecules and Metal Ions in Supramolecular Assembly Induced by Cu2+ and Tb3+ Measured by Gel Electrophoresis Techniques
Sumika Nakano; Kazuki Marumo; Rintaro Kazami; Takumi Saito; Tomoko Haraga; Yuiko Tasaki-Handa; Shingo Saito
Environmental Science & Technology, Volume：55, Number：22, First page：15172, Last page：15180, Nov. 2021, [Reviewed], [Last, Corresponding]
American Chemical Society (ACS), Scientific journal
DOI：https://doi.org/10.1021/acs.est.1c03993
DOI ID：10.1021/acs.est.1c03993, ISSN：0013-936X, eISSN：1520-5851 - Single‐Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein‐Binding DNA Aptamers
Shingo Saito; Toshiki Sakamoto; Naoki Tanaka; Ryo Watanabe; Takuya Kamimura; Kazuki Ota; Kathryn R. Riley; Keitaro Yoshimoto; Yuiko Tasaki‐Handa; Masami Shibukawa
Chemistry – A European Journal, Volume：27, Number：39, First page：10058, Last page：10067, Jul. 2021, [Reviewed], [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1002/chem.202100177
DOI ID：10.1002/chem.202100177, ISSN：0947-6539, eISSN：1521-3765, Web of Science ID：WOS:000659714000001 - Effect of coexisting alkali metal ions on the variation in the coordination mode of 1,4-phenylenbis(methylidyne)tetrakis(phosphonic acid) in a lanthanum(III) metal–organic framework
Yuiko Tasaki-Handa; Masami Shibukawa; Shingo Saito
Inorganic Chemistry Communications, Volume：128, First page：108560, Last page：108560, Jun. 2021, [Reviewed], [Last]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.inoche.2021.108560
DOI ID：10.1016/j.inoche.2021.108560, ISSN：1387-7003 - SELEX-based DNA Aptamer Selection: A Perspective from the Advancement of Separation Techniques
Shingo SAITO
Analytical Sciences, Volume：37, Number：1, First page：17, Last page：26, Jan. 2021, [Reviewed], [Lead, Corresponding]
Japan Society for Analytical Chemistry, Scientific journal
DOI：https://doi.org/10.2116/analsci.20sar18
DOI ID：10.2116/analsci.20sar18, ISSN：0910-6340, eISSN：1348-2246 - Intrinsic difference between phenyl hexyl- and octadecyl-bonded silicas in the solute retention selectivity in reversed-phase liquid chromatography with aqueous mobile phase
Keisuke Nakamura; Shingo Saito; Masami Shibukawa
Journal of Chromatography A, Volume：1628, First page：461450, Last page：461450, Sep. 2020, [Reviewed]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2020.461450
DOI ID：10.1016/j.chroma.2020.461450, ISSN：0021-9673 - Transmetalation in a Ce(III)‐phosphoester Crystalline Coordination Polymer with an Exceptionally High Selectivity for Yb(III) and Lu(III)
Yuiko Tasaki‐Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
Chemistry – An Asian Journal, Volume：15, Number：17, First page：2653, Last page：2659, Jul. 2020, [Reviewed], ［International magazine］
A novel crystalline coordination polymer containing Ce3+ and bis(4-nitrophenyl) phosphate (L), CeL3 , was synthesized and its unique transmetalation selectivities toward Yb3+ and Lu3+ in the lanthanide series were evaluated. The relatively large difference in transmetalation selectivity between the neighboring Tm3+ and Yb3+ species is noteworthy because the reactivities of heavy lanthanides are generally considerably similar. The structural strain of the polymeric framework is likely responsible for this unusual trend. Powder X-ray diffraction analysis indicated that, in the cases of only Yb3+ and Lu3+ , large differences in their ionic sizes compared to that of Ce3+ in the parent framework may induce a structural strain after solid solution formation, while cleavage of the relatively weak Ce-O bond allows the formation of new Yb-O and Lu-O bonds with the incoming Yb3+ and Lu3+ , respectively. Structural phase transitions likely caused by the heterogeneous nucleation of the Yb- (or Lu-) type phase were also observed.
Wiley, English, Scientific journal
DOI：https://doi.org/10.1002/asia.202000502
DOI ID：10.1002/asia.202000502, ISSN：1861-4728, eISSN：1861-471X, PubMed ID：32502320 - Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis
Tomoko Haraga; Hiroto Tsujimura; Saori Miyauchi; Takuya Kamimura; Masami Shibukawa; Shingo Saito
ELECTROPHORESIS, Volume：41, Number：13-14, First page：1152, Last page：1159, Jul. 2020, [Reviewed], [Last, Corresponding]
Wiley, Scientific journal
DOI：https://doi.org/10.1002/elps.201900399
DOI ID：10.1002/elps.201900399, ISSN：0173-0835, eISSN：1522-2683 - Advanced Gel Electrophoresis Techniques Reveal Heterogeneity of Humic Acids Based on Molecular Weight Distributions of Kinetically Inert Cu2+–Humate Complexes
Kazuki Marumo; Atsumasa Matsumoto; Sumika Nakano; Masami Shibukawa; Takumi Saito; Tomoko Haraga; Shingo Saito
Environmental Science & Technology, Volume：53, Number：24, First page：14507, Last page：14515, Nov. 2019, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1021/acs.est.9b05169
DOI ID：10.1021/acs.est.9b05169 - Two-Dimensional Polyacrylamide Gel Electrophoresis for Metalloprotein Analysis Based on Differential Chemical Structure Recognition by CBB Dye
Junko Ishikawa; Akinori Maeshima; Allyson Mellinger; Anne Durand; Marie-Line Bourbon; Daichi Higo; Christa L Colyer; Masami Shibukawa; Soufian Ouchane; Shingo Saito
Scientific Reports, Volume：9, Number：1, First page：10566, Jul. 2019, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1038/s41598-019-46955-6
DOI ID：10.1038/s41598-019-46955-6 - Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX
Koji Wakui; Toru Yoshitomi; Akane Yamaguchi; Maho Tsuchida; Shingo Saito; Masami Shibukawa; Hitoshi Furusho; Keitaro Yoshimoto
Molecular Therapy-Nucleic Acids, Volume：16, First page：348, Last page：359, Jun. 2019, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1016/j.omtn.2019.03.002
DOI ID：10.1016/j.omtn.2019.03.002, ISSN：2162-2531, Web of Science ID：WOS:000470250900031 - Facilitated Dehydration of Rb + Ions in Cation‐Exchange Resin when Surrounded by Cs + Ions: A Marked Phenomenon in Superheated Water
Makoto Harada; Tetsuo Okada; Keisuke Nakamura; Shingo Saito; Masami Shibukawa
ChemistrySelect, Volume：4, Number：16, First page：4718, Last page：4725, Apr. 2019, [Reviewed]
Wiley, English, Scientific journal
DOI：https://doi.org/10.1002/slct.201900388
DOI ID：10.1002/slct.201900388, ISSN：2365-6549, eISSN：2365-6549 - Excess adsorption of acetonitrile and water on MIL-100 (Fe) and its potential application in mixed-mode chromatography
Yuiko Tasaki-Handa; Maho Yoshikawa; Shingo Saito; Masami Shibukawa
New Journal of Chemistry, Volume：43, Number：42, First page：16566, Last page：16571, 2019, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1039/c9nj03981c
DOI ID：10.1039/c9nj03981c, ISSN：1144-0546, eISSN：1369-9261, Web of Science ID：WOS:000493080000016 - Carbon Dot-Mediated Capillary Electrophoresis Separations of Metallated and Demetallated Forms of Transferrin Protein
Leona R. Sirkisoon; Honest C. Makamba; Shingo Saito; Christa L. Colyer
Molecules, Volume：24, Number：10, First page：1916, Jan. 2019, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.3390/molecules24101916
DOI ID：10.3390/molecules24101916 - Effect of Acetonitrile on the Solute Distribution at the Heterogeneous Interface Region between Water and Hydrocarbonaceous Silica Revealed by Surface-Bubble-Modulated Liquid Chromatography
Keisuke Nakamura; Ryusuke Ubukata; Hisashi Mizuno; Shingo Saito; Masami Shibukawa
Journal of Physical Chemistry C, Volume：122, Number：50, First page：28674, Last page：28683, Nov. 2018, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpcc.8b08435
DOI ID：10.1021/acs.jpcc.8b08435 - Adsorption at the Water/Hydrophobe Interface versus Partition into the Interior of the Hydrophobe: Quantitative Evaluation of the Solute Retention Selectivity at the Water/Hydrocarbon Interface (vol 122, pg 4409, 2018)
Nakamura Keisuke; Saito Shingo; Shibukawa Masami
JOURNAL OF PHYSICAL CHEMISTRY C, Volume：122, Number：38, First page：22223, Sep. 2018, [Reviewed]
DOI：https://doi.org/10.1021/acs.jpcc.8b08438
DOI ID：10.1021/acs.jpcc.8b08438, ISSN：1932-7447, Web of Science ID：WOS:000446141900058 - Identification of new classes of thrombin-binding aptamers by microbeads-assisted capillary electrophoresis (MACE) SELEX
Wakui Koji; Yoshitomi Toru; Tsuchida Maho; Saito Shingo; Shibukawa Masami; Furusho Hitoshi; Yoshimoto Keitaro
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Volume：255, Mar. 2018, [Reviewed]
English
ISSN：0065-7727, Web of Science ID：WOS:000435537700330 - Adsorption at the Water/Hydrophobe Interface versus Partition into the Interior of the Hydrophobe: Quantitative Evaluation of the Solute Retention Selectivity at the Water/Hydrocarbon Interface
Keisuke Nakamura; Shingo Saito; Masami Shibukawa
Journal of Physical Chemistry C, Volume：122, Number：8, First page：4409, Last page：4418, Mar. 2018, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/acs.jpcc.7b12413
DOI ID：10.1021/acs.jpcc.7b12413, ISSN：1932-7455, SCOPUS ID：85042746291, Web of Science ID：WOS:000426802500035 - Determination of Free Magnesium Oxide in Steelmaking Slags by Microwave-Assisted-Hydration/Thermogravimetry
Misa Kato; Kenta Tsukagoshi; Michihiro Aimoto; Shingo Saito; Masami Shibukawa
ISIJ International, Volume：58, Number：10, First page：1832, Last page：1837, 2018, [Reviewed]
English, Scientific journal - Partition/Ion-exclusion chromatographic ion stacking for the analysis of trace anions in water and salt samples by ion chromatography
Fouzia Akter; Shingo Saito; Yuiko Tasaki-Handa; Masami Shibukawa
Analytical Sciences, Volume：34, Number：3, First page：369, Last page：373, 2018, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.34.369
DOI ID：10.2116/analsci.34.369, ISSN：1348-2246, SCOPUS ID：85043362628, Web of Science ID：WOS:000429634200018 - Safe and rapid development of capillary electrophoresis for ultratrace uranyl ions in radioactive samples by way of fluorescent probe selection for actinide ions from a chemical library
Tomoko Haraga; Kazuki Ouchi; Yoshiyuki Sato; Hitoshi Hoshino; Rei Tanaka; Takashi Fujihara; Hideki Kurokawa; Masami Shibukawa; Ken-ichiro Ishimori; Yutaka Kameo; Shingo Saito
Analytica Chimica Acta, Volume：1032, First page：188, Last page：196, 2018, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1016/j.aca.2018.05.077
DOI ID：10.1016/j.aca.2018.05.077, ISSN：1873-4324, SCOPUS ID：85048477881, Web of Science ID：WOS:000443002100021 - Synergistic effect of temperature and background counterions on ion-exchange equilibria
Shibukawa Masami; Yanagisawa Masaru; Morinaga Ryota; Shimasaki Tomomi; Saito Shingo; Wang Shao-ting; Feng Yu-qi
RSC ADVANCES, Volume：8, Number：47, First page：26849, Last page：26856, 2018, [Reviewed]
DOI：https://doi.org/10.1039/c8ra03309a
DOI ID：10.1039/c8ra03309a, ISSN：2046-2069, Web of Science ID：WOS:000445745800046 - A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis
Kazuki Hirose; Maho Tsuchida; Hinako Asakura; Koji Wakui; Keitaro Yoshimoto; Keisuke Iida; Makoto Sato; Masami Shibukawa; Masami Suganuma; Shingo Saito
ANALYST, Volume：142, Number：21, First page：4030, Last page：4038, Nov. 2017, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1039/c7an00909g
DOI ID：10.1039/c7an00909g, ISSN：0003-2654, eISSN：1364-5528, Web of Science ID：WOS:000413562100003 - Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract
Takeharu Wada; I. Wayan Sumardika; Shingo Saito; I. Made Winarsa Ruma; Eisaku Kondo; Masami Shibukawa; Masakiyo Sakaguchi
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Volume：1061, First page：209, Last page：219, Sep. 2017, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1016/j.jchromb.2017.07.022
DOI ID：10.1016/j.jchromb.2017.07.022, ISSN：1570-0232, eISSN：1873-376X, Web of Science ID：WOS:000411542000029 - Mechanism of ion stacking in aqueous partition chromatographic processes
Fouzia Akter; Yawara Ogiyama; Shingo Saito; Masami Shibukawa
JOURNAL OF SEPARATION SCIENCE, Volume：40, Number：16, First page：3205, Last page：3213, Aug. 2017, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1002/jssc.201700081
DOI ID：10.1002/jssc.201700081, ISSN：1615-9306, eISSN：1615-9314, Web of Science ID：WOS:000409434100003 - Development of simple and rapid analytical method for radioactive samples by capillary electrophoresis
原賀 智子; 佐藤 義行; 亀尾 裕; 齋藤 伸吾
Dekomisshoningu Giho, Number：55, First page：22, Last page：27, Mar. 2017, [Reviewed], [Last]
Japanese
ISSN：1343-3881, CiNii Articles ID：40021176640, CiNii Books ID：AA11695205 - Development and inter-laboratory study of a method for quantifying ochratoxin A in pet foods
T. Wada; H. Saito; K. Aoyama; S. Saito; M. Shibukawa
WORLD MYCOTOXIN JOURNAL, Volume：10, Number：1, First page：53, Last page：61, 2017, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.3920/WMJ2015.1953
DOI ID：10.3920/WMJ2015.1953, ISSN：1875-0710, eISSN：1875-0796, Web of Science ID：WOS:000396027500005 - Superheated Water Ion-exchange Chromatography
Masami Shibukawa; Ryota Morinaga; Shingo Saito
BUNSEKI KAGAKU, Volume：65, Number：11, First page：615, Last page：623, Nov. 2016, [Reviewed]
Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.65.615
DOI ID：10.2116/bunsekikagaku.65.615, ISSN：0525-1931, Web of Science ID：WOS:000389009900001 - Comparative analysis of distributions of radioactive cesium and potassium and stable cesium, potassium, and strontium in brown rice grains contaminated with radioactive materials released by the Fukushima Daiichi Nuclear Power Plant accident
M. Kato; Y. Okada; S. Hirai; Y. Minai; S. Saito; M. Shibukawa
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, Volume：310, Number：1, First page：247, Last page：252, Oct. 2016, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1007/s10967-016-4824-3
DOI ID：10.1007/s10967-016-4824-3, ISSN：0236-5731, eISSN：1588-2780, Web of Science ID：WOS:000383579900025 - Selective Spectrophotometric Determination of Trace Amounts of Cadmium in Soil and Sediment Samples Using a Green Aqueous Biphasic Extraction
Masami Shibukawa; Daiki Nobushima; Shoko Sakuma; Mari Sasaki; Keisuke Nakamura; Yuma Matsutani; Kazunori Saitoh; Shingo Saito
ANALYTICAL SCIENCES, Volume：32, Number：10, First page：1095, Last page：1100, Oct. 2016, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.32.1095
DOI ID：10.2116/analsci.32.1095, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000386423300011 - Erratum: Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces (Anal. Chem. (2015) 87(1180-1187)DOI: 10.1021/ac503802q)
Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
Analytical Chemistry, Volume：88, First page：1065, Jan. 2016
DOI：https://doi.org/10.1021/acs.analchem.5b04447
DOI ID：10.1021/acs.analchem.5b04447, ISSN：0003-2700, SCOPUS ID：84953439586 - Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis
Shingo Saito; Kazuki Hirose; Maho Tsuchida; Koji Wakui; Keitaro Yoshimoto; Yoshitaka Nishiyama; Masami Shibukawa
CHEMICAL COMMUNICATIONS, Volume：52, Number：3, First page：461, Last page：464, 2016, [Reviewed], [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1039/c5cc07268a
DOI ID：10.1039/c5cc07268a, ISSN：1359-7345, eISSN：1364-548X, Web of Science ID：WOS:000367259200005 - Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces (vol 87, pg 1180, 2015)
Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
ANALYTICAL CHEMISTRY, Volume：88, Number：1, First page：1065, Last page：1065, Jan. 2016, [Reviewed]
English
DOI：https://doi.org/10.1021/acs.analchem.5b04447
DOI ID：10.1021/acs.analchem.5b04447, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000367866100097 - Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes
Daiki Goto; Kazuki Ouchi; Masami Shibukawa; Shingo Saito
ANALYTICAL SCIENCES, Volume：31, Number：11, First page：1143, Last page：1149, Nov. 2015, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.31.1143
DOI ID：10.2116/analsci.31.1143, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000365375700007 - Determination of the cis-trans lsomerization Barriers of L-Alanyl-L-proline in Aqueous Solutions and at Water/Hydrophobic Interfaces by On-Line Temperature-Jump Relaxation HPLC and Dynamic On-Column Reaction HPLC
Masami Shibukawa; Ayaka Miyake; Sayaka Eda; Shingo Saito
ANALYTICAL CHEMISTRY, Volume：87, Number：18, First page：9280, Last page：9287, Sep. 2015, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/acs.analchem.5b02488
DOI ID：10.1021/acs.analchem.5b02488, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000361416000025 - c-Type Cytochrome Assembly Is a Key Target of Copper Toxicity within the Bacterial Periplasm
Anne Durand; Asma Azzouzi; Marie-Line Bourbon; Anne-Soisig Steunou; Sylviane Liotenberg; Akinori Maeshima; Chantal Astier; Manuela Argentini; Shingo Saito; Soufian Ouchane
MBIO, Volume：6, Number：5, Sep. 2015, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1128/mBio.01007-15
DOI ID：10.1128/mBio.01007-15, ISSN：2150-7511, Web of Science ID：WOS:000364523100013 - Molecular Design of Boronic Acid-Functionalized Squarylium Cyanine Dyes for Multiple Discriminant Analysis of Sialic Acid in Biological Samples: Selectivity toward Monosaccharides Controlled by Different Alkyl Side Chain Lengths
Kazuki Ouchi; Christa L. Colyer; Mahmoud Sebaiy; Jin Zhou; Takeshi Maeda; Hiroyuki Nakazumi; Masami Shibukawa; Shingo Saito
ANALYTICAL CHEMISTRY, Volume：87, Number：3, First page：1933, Last page：1940, Feb. 2015, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1021/ac504201b
DOI ID：10.1021/ac504201b, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000349059000071 - Combining capillary electrophoresis and next-generation sequencing for aptamer selection
Kathryn R. Riley; Jason Gagliano; Jiajie Xiao; Kara Libby; Shingo Saito; Guo Yu; Roger Cubicciotti; Jed Macosko; Christa L. Colyer; Martin Guthold; Keith Bonin
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Volume：407, Number：6, First page：1527, Last page：1532, Feb. 2015, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1007/s00216-014-8427-y
DOI ID：10.1007/s00216-014-8427-y, ISSN：1618-2642, eISSN：1618-2650, Web of Science ID：WOS:000349337100001 - Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces
Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
ANALYTICAL CHEMISTRY, Volume：87, Number：2, First page：1180, Last page：1187, Jan. 2015, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/ac503802q
DOI ID：10.1021/ac503802q, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000348332300049 - Facilitating aptamer selection and collection by capillary transient isotachophoresis with laser-induced fluorescence detection
Kathryn R. Riley; Shingo Saito; Jason Gagliano; Christa L. Colyer
JOURNAL OF CHROMATOGRAPHY A, Volume：1368, First page：183, Last page：189, Nov. 2014, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2014.09.062
DOI ID：10.1016/j.chroma.2014.09.062, ISSN：0021-9673, eISSN：1873-3778, Web of Science ID：WOS:000344437500020 - Application of Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Trace Neodymium in Spent Nuclear Fuel Using Complexation with an Emissive Macrocyclic Polyaminocarboxylate Probe
Tomoko Haraga; Shingo Saito; Yoshiyuki Sato; Shiho Asai; Yukiko Hanzawa; Hitoshi Hoshino; Masami Shibukawa; Ken-ichiro Ishimori; Kuniaki Takahashi
ANALYTICAL SCIENCES, Volume：30, Number：7, First page：773, Last page：776, Jul. 2014, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.30.773
DOI ID：10.2116/analsci.30.773, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000339461400011 - Mapping of protein-bound metal ions using novel polyacrylamide electrophoresis
Saito Shingo
SEIBUTSU BUTSURI KAGAKU, Volume：58, Number：2, First page：24, Last page：26, 2014
Japanese Electrophoresis Society, Japanese
DOI：https://doi.org/10.2198/sbk.58.24
DOI ID：10.2198/sbk.58.24, ISSN：0031-9082, CiNii Articles ID：130004704677, CiNii Books ID：AN00129729 - Determination of Free Lime in Steelmaking Slags by Use of Ethylene Glycol Extraction/ICP-AES and Thermogravimetry
Misa Kato; Tatsuya Hari; Shingo Saito; Masami Shibukawa
TETSU TO HAGANE-JOURNAL OF THE IRON AND STEEL INSTITUTE OF JAPAN, Volume：100, Number：3, First page：340, Last page：345, 2014, [Reviewed]
Japanese, Scientific journal
DOI：https://doi.org/10.2355/tetsutohagane.100.340
DOI ID：10.2355/tetsutohagane.100.340, ISSN：0021-1575, eISSN：1883-2954, Web of Science ID：WOS:000332682600002 - Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: Distribution of copper in human serum
Shingo Saito; Mitsuyoshi Kawashima; Hiroki Ohshima; Kazuki Enomoto; Makoto Sato; Hajime Yoshimura; Keitaro Yoshimoto; Mizuo Maeda; Masami Shibukawa
Analyst, Volume：138, Number：20, First page：6097, Last page：6105, Oct. 2013, [Reviewed], [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1039/c3an01107k
DOI ID：10.1039/c3an01107k, ISSN：0003-2654, PubMed ID：23964357, SCOPUS ID：84884222364, Web of Science ID：WOS:000324545000031 - New molecular motif for recognizing sialic acid using emissive lanthanide-macrocyclic polyazacarboxylate complexes: Deprotonation of a coordinated water molecule controls specific binding
Kazuki Ouchi; Shingo Saito; Masami Shibukawa
Inorganic Chemistry, Volume：52, Number：11, First page：6239, Last page：6241, Jun. 2013, [Reviewed], [Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1021/ic400725a
DOI ID：10.1021/ic400725a, ISSN：0020-1669, PubMed ID：23697912, SCOPUS ID：84878636933, Web of Science ID：WOS:000320015700003 - An Application of Polymer-Enhanced Capillary Transient Isotachophoresis with an Emissive Boronic Acid Functionalized Squarylium Dye as an On-Capillary Labeling Agent for Gram-positive Bacteria
Shingo Saito; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
ANALYTICAL SCIENCES, Volume：29, Number：1, First page：157, Last page：159, Jan. 2013, [Reviewed], [Lead]
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.29.157
DOI ID：10.2116/analsci.29.157, ISSN：0910-6340, Web of Science ID：WOS:000314332200029 - Multistep pH-Peak-Focusing Countercurrent Chromatography with a Polyethylene Glycol-Na2SO4 Aqueous Two Phase System for Separation and Enrichment of Rare Earth Elements
Kohei Shimizu; Hiroaki Kuribayashi; Haruna Watanabe; Tomomi Shimasaki; Kenzaburo Azuma; Yohei Horie; Kazunori Saitoh; Shingo Saito; Masami Shibukawa
ANALYTICAL CHEMISTRY, Volume：85, Number：2, First page：978, Last page：984, Jan. 2013, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1021/ac302546s
DOI ID：10.1021/ac302546s, ISSN：0003-2700, Web of Science ID：WOS:000313668400047 - A Long-Wavelength Fluorescent Squarylium Cyanine Dye Possessing Boronic Acid for Sensing Monosaccharides and Glycoproteins with High Enhancement in Aqueous Solution
Shingo Saito; Tara L. Massie; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
SENSORS, Volume：12, Number：5, First page：5420, Last page：5431, May 2012, [Reviewed], [Lead]
English, Scientific journal
DOI：https://doi.org/10.3390/s120505420
DOI ID：10.3390/s120505420, ISSN：1424-8220, Web of Science ID：WOS:000304539200013 - Highly sensitive detection of neodymium ion in small amount of spent nuclear fuel samples using novel fluorescent macrocyclic hexadentate polyaminocarboxylate probe in capillary electrophoresis-laser-induced fluorescence detection
Shingo Saito; Yoshiyuki Sato; Tomoko Haraga; Yuta Nakano; Shiho Asai; Yutaka Kameo; Kuniaki Takahashi; Masami Shibukawa
JOURNAL OF CHROMATOGRAPHY A, Volume：1232, First page：152, Last page：157, Apr. 2012, [Reviewed], [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2011.11.032
DOI ID：10.1016/j.chroma.2011.11.032, ISSN：0021-9673, Web of Science ID：WOS:000302423000021 - On-Column Labeling of Gram-Positive Bacteria with a Boronic Acid Functionalized Squarylium Cyanine Dye for Analysis by Polymer-Enhanced Capillary Transient Isotachophoresis
Shingo Saito; Tara L. Massie; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
ANALYTICAL CHEMISTRY, Volume：84, Number：5, First page：2452, Last page：2458, Mar. 2012, [Reviewed], [Lead]
English, Scientific journal
DOI：https://doi.org/10.1021/ac2031145
DOI ID：10.1021/ac2031145, ISSN：0003-2700, Web of Science ID：WOS:000301021400050 - Capillary electrophoresis with laser-induced fluorescent detection method using highly emissive probes for analysis of uranium in radioactive wastes
HARAGA TOMOKO; SATO YOSHIYUKI; SHIBUKAWA MASAMI; KAMEO YUTAKA; TAKAHASHI KUNIAKI; SAITO SHINGO
Proceedings of Annual / Fall Meetings of Atomic Energy Society of Japan, Volume：2012, First page：458, Last page：458, 2012
放射性廃棄物の処分に向けて、廃棄物試料中のアクチニド（Th、U、Np、Pu、Am、Cm）を簡易かつ高感度に分析する方法を開発することを目的として、キャピラリー電気泳動－レーザー励起蛍光検出法（CE-LIF）の適用性について検討している。本検討では、ウランを分析対象として、ウラニルイオンと安定な錯体を形成する配位骨格を有する蛍光プローブを新規に合成し、CE-LIFによりウラニルイオンの分離検出を試みた。その結果、溶液試料において、従来のCE法の検出限界（数十ppb－ppmレベル）を大幅に改善した数十pptレベルの検出限界を達成できた。
Atomic Energy Society of Japan, Japanese
DOI：https://doi.org/10.11561/aesj.2012f.0.458.0
DOI ID：10.11561/aesj.2012f.0.458.0, CiNii Articles ID：130005051757 - XAFS Study of Thermal Effect on Hydration Structures of Inorganic Ions
Masami Shibukawa; Yawara Ogiyama; Tomomi Shimasaki; Yoshiki Kondo; Asako Inoue; Shingo Saito
Photon Factory Activity Report, 2012 - X-Ray absorption fine structure spectroscopy studies of thermal effects on ion-exchange equilibria
Masami Shibukawa; Makoto Harada; Tetsuo Okada; Yawara Ogiyama; Tomomi Shimasaki; Yoshiki Kondo; Asako Inoue; Shingo Saito
RSC ADVANCES, Volume：2, Number：24, First page：8985, Last page：8991, 2012, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1039/c2ra21278a
DOI ID：10.1039/c2ra21278a, ISSN：2046-2069, Web of Science ID：WOS:000308824600018 - Determination of Trace Amount of Cobalt in a Steel Sample by Two-dimensional On-line Redox Derivatization Liquid Chromatography
Masami Shibukawa; Akira Yamazaki; Shingo Saito; Koichi Saitoh
ISIJ INTERNATIONAL, Volume：52, Number：9, First page：1622, Last page：1626, 2012, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.2355/isijinternational.52.1622
DOI ID：10.2355/isijinternational.52.1622, ISSN：0915-1559, Web of Science ID：WOS:000309297800015 - Total Design of Novel Fluorescent Probes and Kinetically Integrated Chemical Systems for the Separation and Detection of Metal Ions
Shingo Saito
BUNSEKI KAGAKU, Volume：60, Number：10, First page：773, Last page：784, Oct. 2011, [Reviewed], [Lead, Corresponding]
Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.60.773
DOI ID：10.2116/bunsekikagaku.60.773, ISSN：0525-1931, Web of Science ID：WOS:000296316500001 - Reversed-phase ion-pair liquid chromatographic method for determination of reaction equilibria involving ionic species: Exemplification of the method using ligand substitution reactions of ethylenediaminetetraacetatochromium(III) ion with acetate and phosphate ions
Emiko Sato; Seiko Miya; Kazunori Saitoh; Shingo Saito; Masami Shibukawa
JOURNAL OF CHROMATOGRAPHY A, Volume：1218, Number：7, First page：922, Last page：928, Feb. 2011, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2010.12.063
DOI ID：10.1016/j.chroma.2010.12.063, ISSN：0021-9673, Web of Science ID：WOS:000287281200006 - Capillary electrophoresis with laser-induced fluorescent detection method using highly emissive probes for analysis of actinides in radioactive wastes
Tomoko Haraga; Yuta Nakano; Masami Shibukawa; Yutaka Kameo; Kuniaki Takahashi; Shingo Saito
Proceedings of the International Conference on Radioactive Waste Management and Environmental Remediation, ICEM, First page：1461, Last page：1465, 2011, [Reviewed], [Corresponding]
English, International conference proceedings
DOI：https://doi.org/10.1115/ICEM2011-59092
DOI ID：10.1115/ICEM2011-59092, SCOPUS ID：84882942012 - Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers
Shingo Saito; Yuta Nakano; Atsushi Hikichi; Ryouji Suzuki; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama; Masami Shibukawa
ANALYST, Volume：136, Number：13, First page：2697, Last page：2705, 2011, [Reviewed], [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1039/c0an00777c
DOI ID：10.1039/c0an00777c, ISSN：0003-2654, eISSN：1364-5528, Web of Science ID：WOS:000291611800004 - Interfacial water on hydrophobic surfaces recognized by ions and molecules
Masami Shibukawa; Yoshiki Kondo; Yawara Ogiyama; Keita Osuga; Shingo Saito
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Volume：13, Number：35, First page：15925, Last page：15935, 2011, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1039/c1cp20704k
DOI ID：10.1039/c1cp20704k, ISSN：1463-9076, eISSN：1463-9084, Web of Science ID：WOS:000294167700024 - Removal of Cr(VI) from aqueous solutions by the culm of bamboo grass treated with concentrated sulfuric acid
M. Koroki; S. Saito; H. Hashimoto; T. Yamada; Masakazu Aoyama
ENVIRONMENTAL CHEMISTRY LETTERS, Volume：8, Number：1, First page：59, Last page：61, Mar. 2010, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1007/s10311-008-0191-9
DOI ID：10.1007/s10311-008-0191-9, ISSN：1610-3653, Web of Science ID：WOS:000275166200008 - Simple spectrophotometric determination of trace amounts of zinc in environmental water samples using aqueous biphasic extraction
Masami Shibukawa; Daisuke Shirota; Shingo Saito; Shin Nagasawa; Kazunori Saitoh; Hiroaki Minamisawa
Bunseki Kagaku, Volume：59, Number：10, First page：847, Last page：854, 2010
Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.59.847
DOI ID：10.2116/bunsekikagaku.59.847, ISSN：0525-1931, SCOPUS ID：78650457630 - Superheated Water Ion-Exchange Chromatography: An Experimental Approach for Interpretation of Separation Selectivity in Ion-Exchange Processes
Masami Shibukawa; Tomomi Shimasaki; Shingo Saito; Takashi Yarita
ANALYTICAL CHEMISTRY, Volume：81, Number：19, First page：8025, Last page：8032, Oct. 2009
English, Scientific journal
DOI：https://doi.org/10.1021/ac9011864
DOI ID：10.1021/ac9011864, ISSN：0003-2700, CiNii Articles ID：80020661337, PubMed ID：19743878, Web of Science ID：WOS:000270361100026 - Superheated Water Ion-Exchange Chromatography: An Experimental Approach for Interpretation of Separation Selectivity in Ion-Exchange Processes
Masami Shibukawa; Tomomi Shimasaki; Shingo Saito; Takashi Yarita
ANALYTICAL CHEMISTRY, Volume：81, Number：19, First page：8025, Last page：8032, Oct. 2009
English, Scientific journal
DOI：https://doi.org/10.1021/ac9011864
DOI ID：10.1021/ac9011864, ISSN：0003-2700, Web of Science ID：WOS:000270361100026 - Recognition of Monosaccharides with Energy-transfer Luminescence Using Residual Coordination Sites of Lanthanide(III)-4-Aminobenzyl-EDTA Complex in Aqueous Solution
Shingo Saito; Atsushi Hikichi; Takao Kamura; Kazuyuki Hattori; Masakazu Aoyama; Masami Shibukawa
CHEMISTRY LETTERS, Volume：38, Number：5, First page：412, Last page：413, May 2009, [Lead, Corresponding]
English, Scientific journal
DOI：https://doi.org/10.1246/cl.2009.412
DOI ID：10.1246/cl.2009.412, ISSN：0366-7022, eISSN：1348-0715, CiNii Articles ID：10025121487, Web of Science ID：WOS:000267362100012 - Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex
Hiroshi Atsumi; Keitaro Yoshimoto; Shingo Saito; Moriya Ohkuma; Mizuo Maeda; Yukio Nagasaki
TETRAHEDRON LETTERS, Volume：50, Number：19, First page：2177, Last page：2180, May 2009
English, Scientific journal
DOI：https://doi.org/10.1016/j.tetlet.2009.02.152
DOI ID：10.1016/j.tetlet.2009.02.152, ISSN：0040-4039, eISSN：1873-3581, CiNii Articles ID：120001300353, Web of Science ID：WOS:000265136000004 - Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex
Hiroshi Atsumi; Keitaro Yoshimoto; Shingo Saito; Moriya Ohkuma; Mizuo Maeda; Yukio Nagasaki
TETRAHEDRON LETTERS, Volume：50, Number：19, First page：2177, Last page：2180, May 2009, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1016/j.tetlet.2009.02.152
DOI ID：10.1016/j.tetlet.2009.02.152, ISSN：0040-4039, eISSN：1873-3581, Web of Science ID：WOS:000265136000004 - Separation selectivity of aqueous polyethylene glycol-based separation systems: DSC, LC and aqueous two-phase extraction studies
Masami Shibukawa; Ryoichi Ichikawa; Takayuki Baba; Ryosaku Sakamoto; Shingo Saito; Koichi Oguma
POLYMER, Volume：49, Number：19, First page：4168, Last page：4173, Sep. 2008
English, Scientific journal
DOI：https://doi.org/10.1016/j.polymer.2008.07.042
DOI ID：10.1016/j.polymer.2008.07.042, ISSN：0032-3861, eISSN：1873-2291, CiNii Articles ID：80019775078, Web of Science ID：WOS:000259303900019 - Control of the contaminant level for determination of Al3+ using 8-quinolinol by high-performance liquid chromatography with fluorescence detection
Shingo Saito; Jun-ichi Tamura; Makoto Sato; Masakazu Aoyama
JOURNAL OF CHROMATOGRAPHY A, Volume：1190, Number：1-2, First page：198, Last page：203, May 2008
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2008.03.018
DOI ID：10.1016/j.chroma.2008.03.018, ISSN：0021-9673, CiNii Articles ID：80019615886, Web of Science ID：WOS:000255848600024 - Solubilization of polystyrene into monoterpenes
Kazuyuki Hattori; Satofumi Naito; Kenji Yamauchi; Hisayuki Nakatani; Takashi Yoshida; Shingo Saito; Masakazu Aoyama; Tetsuo Miyakoshi
Advances in Polymer Technology, Volume：27, Number：1, First page：35, Last page：39, 2008
English, Scientific journal
DOI：https://doi.org/10.1002/adv.20115
DOI ID：10.1002/adv.20115, ISSN：0730-6679, CiNii Articles ID：80020036030, SCOPUS ID：57549113705 - Inhibitory effects of L-pipecolic acid from the edible mushroom, Sarcodon aspratus, on angiotensin I-converting enzyme
Masaaki Kiyoto; Shingo Saito; Kazuyuki Hattori; Nam-Seok Cho; Takaaki Hara; Yuzo Yagi; Masakazu Aoyama
JOURNAL OF WOOD SCIENCE, Volume：54, Number：2, First page：179, Last page：181, 2008
English, Scientific journal
DOI：https://doi.org/10.1007/s10086-007-0923-7
DOI ID：10.1007/s10086-007-0923-7, ISSN：1435-0211, Web of Science ID：WOS:000256405100014 - Removal of Cr(VI) by the bark of Quercus dentate treated with concentrated sulfuric acid
Motoki Koroki; Yukihiro Hashimoto; Shingo Saito; Tetuo Yamada; Harumi Hashimoto; Masakazu Aoyama
Wood Carbonization Research, Volume：4, Number：2, First page：55, Last page：61, 2008 - Sorption of Cr(VI) on the wood of Japanese larch treated with concentrated sulfuric acid
Masakazu Aoyama; Shingo Saito; Masaaki Tagami
JOURNAL OF WOOD SCIENCE, Volume：53, Number：6, First page：545, Last page：549, Dec. 2007
English, Scientific journal
DOI：https://doi.org/10.1007/s10086-007-0898-4
DOI ID：10.1007/s10086-007-0898-4, ISSN：1435-0211, CiNii Articles ID：10022025247, Web of Science ID：WOS:000253284300015 - Direct fluorometric detection of paramagnetic and heavy metal ions at sub-amol level using an aromatic polyaminocarboxylate by CZE: Combination of pre- and on-capillary complexation technique
Shingo Saito; Ryouji Suzuki; Naokj Danzaka; Atsushi Hikichi; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
ELECTROPHORESIS, Volume：28, Number：14, First page：2448, Last page：2457, Jul. 2007
English, Scientific journal
DOI：https://doi.org/10.1002/elps.200600516
DOI ID：10.1002/elps.200600516, ISSN：0173-0835, eISSN：1522-2683, CiNii Articles ID：120001370544, PubMed ID：17577884, Web of Science ID：WOS:000248390900016 - Fluorescence-based affinity labeling of nucleobase by hydrogen-bond forming metal complex
Keitaro Yoshimoto; Hiroshi Atsumi; Shingo Saito; Moriya Okuma; Mizuo Maeda; Yukio Nagasaki
Nucleic Acids Symp. Ser., Volume：51, First page：303, Last page：304, 2007 - Synthesis of bis(alk-3-en-1-ynyl)benzene with either E- or Z-configuration via a one-pot three-component coupling reaction and its optical properties
Masayuki Hoshi; Souichi Suzuki; Shingo Saitoh; Mitsuhiro Okimoto; Kazuya Shirakawa
TETRAHEDRON LETTERS, Volume：48, Number：1, First page：119, Last page：124, Jan. 2007
English, Scientific journal
DOI：https://doi.org/10.1016/j.tetlet.2006.10.162
DOI ID：10.1016/j.tetlet.2006.10.162, ISSN：0040-4039, CiNii Articles ID：80018734376, Web of Science ID：WOS:000243630300025 - Antihypertensive Components in the Fruit Body of Ganoderma lucidum
Masakazu Aoyama; Shingo Saito; Masaaki Kiyoto; Iwao Togashi; Takaaki Hara; Yuzo Yagi; Teruhisa Kimura; Shozo Yoneyama
Mushroom Sci. Biotechnol., Volume：15, Number：2, First page：91, Last page：96, 2007
DOI：https://doi.org/10.24465/msb.15.2_91
DOI ID：10.24465/msb.15.2_91 - Direct fluorescence detection of ultratrace lanthanide(III) ions complexed with aromatic polyaminocarboxylate, avoiding quenching of ligand-centered emission, using capillary zone electrophoresis with a ternary complexing technique
Shingo Saito; Shingo Takeuchi; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
ANALYST, Volume：132, Number：3, First page：237, Last page：241, 2007
English, Scientific journal
DOI：https://doi.org/10.1039/b613534j
DOI ID：10.1039/b613534j, ISSN：0003-2654, eISSN：1364-5528, CiNii Articles ID：120001370745, PubMed ID：17325757, Web of Science ID：WOS:000244487700009 - Selective ultratrace detection of Al(III) and Ga(III) complexed with a calcein isomer by capillary zone electrophoresis with laser-induced fluorescence detection - Comparison of emissive polyaminocarboxylates as derivatizing ligands
Shingo Saito; Jun-ichi Shimidzu; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
JOURNAL OF CHROMATOGRAPHY A, Volume：1140, Number：1-2, First page：230, Last page：235, Jan. 2007
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2006.12.001
DOI ID：10.1016/j.chroma.2006.12.001, ISSN：0021-9673, CiNii Articles ID：120000786948, PubMed ID：17178417, Web of Science ID：WOS:000243953300032 - Fluorescent-based SNP typing using hydrogen bond-forming small ligand and its lanthanide(III) complex
Keitaxo Yoshimoto; Hiroshi Atsumi; Seiichi Nishizawa; Shingo Saito; Hiroyuki Koshino; Moriya Okuma; Mizuo Maeda; Norio Teramae; Yukio Nagasaki
PROGRESS ON POST-GENOME TECHNOLOGIES, First page：22, Last page：23, 2007, [Reviewed]
English, International conference proceedings
Web of Science ID：WOS:000251162700016 - Highly sensitive determination of lanthanides by capillary electrophoresis with direct visible detection after precapillary complexation with aromatic polyaminocarboxylate and additionally applying dynamic ternary complexation with nitrilotriacetic acid
Shingo Saito; Naoki Danzaka; Suwaru Hoshi
ELECTROPHORESIS, Volume：27, Number：15, First page：3093, Last page：3100, Aug. 2006
English, Scientific journal
DOI：https://doi.org/10.1002/elps.200500828
DOI ID：10.1002/elps.200500828, ISSN：0173-0835, CiNii Articles ID：120001370629, PubMed ID：16800028, Web of Science ID：WOS:000239912600012 - Direct fluorescence detection of Pb2+ and Cd2+ by high-performance liquid chromatography using 1-(4-aminobenzyl)ethylenediamine-N,N,N ',N-tetraacetate as a pre-column derivatizing agent
S Saito; N Danzaka; S Hoshi
JOURNAL OF CHROMATOGRAPHY A, Volume：1104, Number：1-2, First page：140, Last page：144, Feb. 2006
English, Scientific journal
DOI：https://doi.org/10.1016/j.chroma.2005.11.085
DOI ID：10.1016/j.chroma.2005.11.085, ISSN：0021-9673, CiNii Articles ID：80017642843, PubMed ID：16343515, Web of Science ID：WOS:000235100300019 - Chemical suppression of contaminant metal ions using a metastable state in precolumn derivatizing HPLC: An ultratrace fluorometric detection of Al(III)
S Saito; T Anada; S Noshi; K Akatsuka
ANALYTICAL CHEMISTRY, Volume：77, Number：16, First page：5332, Last page：5338, Aug. 2005
English, Scientific journal
DOI：https://doi.org/10.1021/ac050380c
DOI ID：10.1021/ac050380c, ISSN：0003-2700, CiNii Articles ID：80017543888, PubMed ID：16097776, Web of Science ID：WOS:000231236300035 - Simultaneous detection of [metal(II)-tpen](2+) as kinetically inert cationic complexes using pre-capillary derivatization electrophoresis: an application to biological samples
S Saito; S Sasamura; S Hoshi
ANALYST, Volume：130, Number：5, First page：659, Last page：663, 2005
English, Scientific journal
DOI：https://doi.org/10.1039/b417394e
DOI ID：10.1039/b417394e, ISSN：0003-2654, CiNii Articles ID：120001370814, PubMed ID：15852134, Web of Science ID：WOS:000228689600008 - Highly-sensitive simultaneous detection of lanthanide(III) ions as kinetically stable aromatic polyaminocarboxylato complexes via capillary electrophoresis using resolution enhancement with carbonate ion
S Saito; H Hoshino
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Volume：378, Number：6, First page：1644, Last page：1647, Mar. 2004, [Reviewed]
English, Scientific journal
DOI：https://doi.org/10.1007/s00216-003-2454-4
DOI ID：10.1007/s00216-003-2454-4, ISSN：1618-2642, PubMed ID：15214429, Web of Science ID：WOS:000220661800031 - Formation reactions and photophysical parameters of highly luminescent lanthanoids(III) complexes with 4-hydroxypyridine-2,6-dicarboxylic acid
N Takahashi; S Saito; H Hoshino
BUNSEKI KAGAKU, Volume：52, Number：9, First page：713, Last page：718, Sep. 2003, [Reviewed]
Japanese, Scientific journal
ISSN：0525-1931, Web of Science ID：WOS:000185447700010 - 4-ヒドロキシピリジン-2,6-ジカルボン酸-ランタノイド（III）錯体の生成反応とエネルギー移動発光挙動
高橋 伸幸; 斎藤 伸吾; 星野 仁
分析化学, Volume：52, Number：9, First page：713, Last page：718, 2003
DOI：https://doi.org/10.2116/bunsekikagaku.52.713
DOI ID：10.2116/bunsekikagaku.52.713, ISSN：0525-1931, CiNii Articles ID：110002905278 - 解離不活性ランタノイド－ポリアミノカルボン酸錯体を用いる高性能分析法の開発
齋藤伸吾
分析化学, Volume：52, Number：1, First page：81, Last page：82, 2003
Analytical methods of Ln^III were developed in which the kinetic inertness of Ln^III complexes with acyclic polyaminocarboxylate (PAC) on the dissociation reaction was the key to establishing detection systems. First, a pre-derivatization HPLC method with fluorescent detection was developed for closedshell Ln^III using an aromatic octadentate PAC as the derivatizing reagent. Selective detection at ppb levels was achieved. Second, the factors to form inert Ln-pac complexes were investigated on the dissociation reaction. The rate constants and activation parameters were determined for a series of Ln^III-hexadentate and octadentate PAC complexes. Interestingly, an unusually large negative activation entropy for solvolysis governed the kinetic inertness, and was probably derived from metal envelopment in the complex and hydration on the activation state. Third, a novel measurement method of the dissociation rate was developed using capillary electrophoresis (CE). The dissociation rate constant for the Ce^III-PAC complex was determined for the first time. Fourth, based on the result of the second study, a sensitive pre-derivatizing CE method for Ln^III at the fmol level was developed using an aromatic EDTA derivative. In this method, a high resolution was achieved by introducing a ternary complex equilibrium in the carrier. Finally, the energy-transfer luminescence of a Tb^III complex with a PAC possessing phenol function was studied. The detection limit at the ppt level was achieved, and the photophysics was also investigated.
Japanese
CiNii Articles ID：110002905162, CiNii Books ID：AN00222633 - Visual fluorimetry of trace lead using ion-pair extraction onto an octadecylsilanized silica disk
J Isoe; Y Sakai; E Kaneko; S Saito; S Hoshi; K Akatsuka
BUNSEKI KAGAKU, Volume：51, Number：11, First page：1037, Last page：1040, Nov. 2002, [Reviewed]
Chinese, Scientific journal
ISSN：0525-1931, Web of Science ID：WOS:000179235800006 - Thin layer chromatographic behavior of a lanthanum-alizarin complexone-fluoride ternary complex
S Arasaka; E Kaneko; S Saito; S Hoshi; K Akatsuka
BUNSEKI KAGAKU, Volume：51, Number：9, First page：837, Last page：839, Sep. 2002, [Reviewed]
Japanese, Scientific journal
ISSN：0525-1931, Web of Science ID：WOS:000178045100028 - Retention behavior of cationic aluminium chelate with o,o '-dihydroxyazobenzene in HPLC using the C-18-bonded silica stationary phase
T Kurasawa; T Yotsuyanagi; E Kaneko; S Saito; S Hoshi; K Akatsuka
BUNSEKI KAGAKU, Volume：51, Number：9, First page：833, Last page：836, Sep. 2002, [Reviewed]
Japanese, Scientific journal
ISSN：0525-1931, Web of Science ID：WOS:000178045100027 - Visual fluorimetry of trace lead using ion-pair extraction onto an octadecylsilanized silica disk
Junichi Isoe; Yasuhiro Sakai; Emiko Kaneko; Shingo Saito; Suwaru Hoshi; Kunihiko Akatsuka
Bunseki Kagaku, Volume：51, Number：11, First page：1037, Last page：1040, 2002
Japan Society for Analytical Chemistry, Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.51.1037
DOI ID：10.2116/bunsekikagaku.51.1037, ISSN：0525-1931, SCOPUS ID：0036440272 - Retention behavior of cationic aluminium chelate with o,o′- dihydroxyazobenzene in HPLC using the C18-bonded silica stationary phase
Takashi Kurasawa; Takao Yotsuyanagi; Emiko Kaneko; Shingo Saito; Suwaru Hoshi; Kunihiko Akatsuka
Bunseki Kagaku, Volume：51, Number：9, First page：833, Last page：836, 2002
Japan Society for Analytical Chemistry, Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.51.833
DOI ID：10.2116/bunsekikagaku.51.833, ISSN：0525-1931, SCOPUS ID：0036391532 - Thin layer chromatographic behavior of a lanthanum-alizarin complexone-fluoride ternary complex
Shingo Arasaka; Emiko Kaneko; Shingo Saito; Suwaru Hoshi; Kunihiko Akatsuka
Bunseki Kagaku, Volume：51, Number：9, First page：837, Last page：839, 2002
Japan Society for Analytical Chemistry, Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.51.837
DOI ID：10.2116/bunsekikagaku.51.837, ISSN：0525-1931, CiNii Articles ID：110002905144, SCOPUS ID：4143081672 - Entropy-controlled solvolytic dissociation kinetics of lanthanide(III) complexes with polyaminocarboxylates in aqueous solutions
S. Saito; H. Hoshino; T. Yotsuyanagi
Inorganic Chemistry, Volume：40, Number：15, First page：3819, Last page：3823, Jul. 2001
English, Scientific journal
DOI：https://doi.org/10.1021/ic001053k
DOI ID：10.1021/ic001053k, ISSN：0020-1669, CiNii Articles ID：80012522035, PubMed ID：11442383, SCOPUS ID：0035898073 - Fluorescence determination of closed-shell rare earth metal ions by reversed-phase HPLC with precolumn derivatization using 8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N ',N '-tetraacetate
S Saito; WZ Xu; H Hoshino; T Yotsuyanagi
BUNSEKI KAGAKU, Volume：50, Number：2, First page：113, Last page：117, Feb. 2001, [Reviewed]
Japanese, Scientific journal
ISSN：0525-1931, Web of Science ID：WOS:000167071300003 - Fluorescence determination of closed-shell rare earth metal ions by reversed-phase HPLC with precolumn derivatization using 8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetate
Shingo Saito; Wen-Zhe Xu; Hitoshi Hoshino; Takao Yotsuyanagi
Bunseki Kagaku, Volume：50, Number：2, First page：113, Last page：117, 2001
Japan Society for Analytical Chemistry, Japanese, Scientific journal
DOI：https://doi.org/10.2116/bunsekikagaku.50.113
DOI ID：10.2116/bunsekikagaku.50.113, ISSN：0525-1931, CiNii Articles ID：110002907394, SCOPUS ID：0035586503 - Dissociation rate constant estimation for the cerium(III)-O,O '-bis(2-aminoethyl)-ethyleneglycol-N,N,N ',N '-tetraacetate system by capillary electrophoresis
S Saito; H Hoshino; T Yotsuyanagi
ANALYTICAL SCIENCES, Volume：16, Number：10, First page：1095, Last page：1097, Oct. 2000
English, Scientific journal
DOI：https://doi.org/10.2116/analsci.16.1095
DOI ID：10.2116/analsci.16.1095, ISSN：0910-6340, CiNii Articles ID：10006675735, Web of Science ID：WOS:000090111000020 - Highly sensitive energy-transfer luminescence of the N,N '-bis(2-hydroxybenzyl)-ethylenediamine-N,N '-diacetatoterbium(III) complex in aqueous solution
S Saito; H Hoshino; T Yotsuyanagi
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, Volume：73, Number：8, First page：1817, Last page：1821, Aug. 2000
English, Scientific journal
DOI：https://doi.org/10.1246/bcsj.73.1817
DOI ID：10.1246/bcsj.73.1817, ISSN：0009-2673, eISSN：1348-0634, CiNii Articles ID：10009152815, Web of Science ID：WOS:000089388600014
- Supplementary Cover: Reliable Post-SELEX Design of Promising Bivalent Aptamers through the Systematic Capillary Electrophoresis Exploration of Ternary DNA–Protein Complexes
Yota Suzuki; Hirochika Tsunehara; Saori Miyauchi; Masato Miyakawa; Kai Sugiyama; Masahiko Ishimitsu; Yuiko Tasaki-Handa; Keitaro Yoshimoto; Shingo Saito
May 2025 - Front Cover (Hot Article): Holo/apo conversion two-dimensional urea PAGE for speciation of Fe3+-bound transferrin in serum
Analytical Sciences, Feb. 2024 - Inside Cover (selected article): Americium(III)/Curium(III) Complete Separation and Sensitive Fluorescence Detection by Capillary and Gel Electrophoresis Using Emissive Hexadentate/Octadentate Polyaminocarboxylate Ligands
Shingo Saito
Bulletin of the Chemical Society of Japan, Mar. 2023, [Lead, Corresponding]
The Chemical Society of Japan
ISSN：0009-2673, eISSN：1348-0634 - Supplemental Cover: Unusually kinetically inert monocationic neptunyl complex with a fluorescein-modified 1,10-phenanthroline-2,9-dicarboxylate ligand: Specific separation and detection in gel electrophoresis
Kazuhito Yamagata; Kazuki Marumo; Yuiko Tasaki-Handa; Tomoko Haraga; Shingo Saito
Inorganic Chemistry, Jan. 2023, [Corresponding]
American Chemical Society (ACS)
DOI：https://doi.org/10.1021/acs.inorgchem.2c02908
DOI ID：10.1021/acs.inorgchem.2c02908, ISSN：0020-1669, eISSN：1520-510X - Cover Feature: Single‐Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein‐Binding DNA Aptamers (Chem. Eur. J. 39/2021)
Shingo Saito; Toshiki Sakamoto; Naoki Tanaka; Ryo Watanabe; Takuya Kamimura; Kazuki Ota; Kathryn R. Riley; Keitaro Yoshimoto; Yuiko Tasaki‐Handa; Masami Shibukawa
Chemistry – A European Journal, Volume：27, Number：39, First page：9963, Last page：9963, 12 Jul. 2021, [Corresponding]
Wiley
DOI：https://doi.org/10.1002/chem.202102064
DOI ID：10.1002/chem.202102064, ISSN：0947-6539, eISSN：1521-3765 - Front cover: Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis
Tomoko Haraga; Hiroto Tsujimura; Saori Miyauchi; Takuya Kamimura; Masami Shibukawa; Shingo Saito
ELECTROPHORESIS, Volume：41, Number：13-14, First page：1152, Last page：1159, Jul. 2020, [Corresponding]
Wiley
DOI：https://doi.org/10.1002/elps.202070071
DOI ID：10.1002/elps.202070071, ISSN：0173-0835, eISSN：1522-2683 - 超高温水におけるイオンの水和とイオン交換の選択性
原田誠; 岡田哲男; 中村圭介; 齋藤伸吾; 渋川雅美
Volume：34th, 2019
J-Global ID：201902275737674036 - 表面気泡変調液体クロマトグラフィーによるアセトニトリル水溶液/C18シリカ界面における物質分離機構の解析-界面における物質分布におよぼす溶媒組成の影響の解明-
中村圭介; 生形竜介; 水野恒; 齋藤伸吾; 渋川雅美
Volume：79th, 2019
J-Global ID：201902224568622010 - Front cover: A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis
The Analyst, Volume：142, Number：21, First page：3997, Last page：3997, 2017, [Corresponding]
Royal Society of Chemistry (RSC)
DOI：https://doi.org/10.1039/c7an90081c
DOI ID：10.1039/c7an90081c, ISSN：0003-2654, eISSN：1364-5528 - 表面気泡変調液体クロマトグラフィーによる逆相分離系の溶質分離選択性におよぼす移動相組成の影響の解明
中村圭介; 生形竜介; 齋藤伸吾; 渋川雅美
Volume：66th, 2017
J-Global ID：201702223555721156 - Rapid Selection System for DNA Aptamers Selectively Binding Microbe Cell Surface by Polymer-Enhanced Capillary Transient Isotachophoresis
齋藤 伸吾
Volume：67, Number：7, First page：505, Last page：512, Jul. 2016
Japanese
ISSN：0451-2014, CiNii Articles ID：40020880448, CiNii Books ID：AN00037245 - Inside back cover: Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis
Chemical Communications, Volume：52, Number：3, First page：622, Last page：622, 2016, [Corresponding]
Royal Society of Chemistry (RSC)
DOI：https://doi.org/10.1039/c6cc90014c
DOI ID：10.1039/c6cc90014c, ISSN：1359-7345, eISSN：1364-548X - 表面気泡変調液体クロマトグラフィーによるアルキル結合型シリカを用いた逆相分離系における溶質保持に対する水/疎水界面およびアルキル結合層の寄与の評価
中村圭介; 齋藤伸吾; 渋川雅美
Volume：76th, 2016
J-Global ID：201602229547081494 - 表面気泡変調液体クロマトグラフィーによる逆相分離系における溶質分離選択性に及ぼす充填剤表面化学構造の影響の解明
中村圭介; 齋藤伸吾; 渋川雅美
Volume：65th, 2016
J-Global ID：201602249663885518 - Measurement of Distribution of Protein-Bound Metal Ions in Biological Samples by Gel Electrophoresis
齋藤 伸吾
Volume：66, Number：1, First page：21, Last page：27, Jan. 2015
Japanese
ISSN：0451-2014, CiNii Articles ID：40020308059, CiNii Books ID：AN00037245 - 表面気泡変調液体クロマトグラフィーによる疎水性表面における水の分離機能の解析
中村圭介; 齋藤伸吾; 渋川雅美
Volume：75th, 2015
J-Global ID：201502219832148964 - 玄米中の各部位における放射性および非放射性セシウムの分析
加藤美佐; 岡田往子; 齋藤伸吾; 渋川雅美
日本分析化学会年会講演要旨集, Volume：63rd, First page：47, Last page：47, 03 Sep. 2014
(公社)日本分析化学会, Japanese
医中誌Web ID：2016161152, J-Global ID：201402204280764465 - 気液ハイブリッド固定相HPLCシステムの開発-分離機構の解明と分離能の評価-
中村圭介; 齋藤伸吾; 渋川雅美
Volume：74th, 2014
J-Global ID：201402225589948568 - 気液ハイブリッド固定相分離システム構築のための気相形成方法の検討
中村圭介; 仲村大樹; 齋藤伸吾; 渋川雅美
Volume：73rd, 2013
J-Global ID：201302229858922536 - フッ化炭素修飾シリカカラムを用いた高性能気液ハイブリッド分離システムの開発
中村圭介; 仲村大樹; 齋藤伸吾; 渋川雅美
Volume：61st, 2012
J-Global ID：201202234079560217 - Front cover: Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers
The Analyst, Volume：136, Number：13, First page：2657, Last page：2657, 2011, [Corresponding]
Royal Society of Chemistry (RSC)
DOI：https://doi.org/10.1039/c1an90042k
DOI ID：10.1039/c1an90042k, ISSN：0003-2654, eISSN：1364-5528 - 剛性の異なる配位骨格を有する八座蛍光配位子錯体のPAGEにおける泳動挙動の調査
榎本七基; 野村高弘; 吉本敬太郎; 佐藤誠; 齋藤伸吾; 前田瑞夫; 渋川雅美
日本分析化学会年会講演要旨集, Volume：57th, First page：353, 27 Aug. 2008
Japanese
J-Global ID：200902244874961904 - ポリアクリルアミドゲル電気泳動による金属イオンの高感度蛍光検出システムの開発
齋藤伸吾; 榎本七基; 野村高弘; 吉本敬太郎; 佐藤誠; 前田瑞夫; 渋川雅美
分析化学討論会講演要旨集, Volume：69th, First page：33, 01 May 2008
Japanese
J-Global ID：200902228170266377 - 水素結合性ランタノイド錯体を用いた新規核酸蛍光ラベリング法の構築
厚見宙志; 吉本敬太郎; 斎藤伸吾; 大熊盛也; 前田瑞夫; 長崎幸夫
生体機能関連化学シンポジウム講演要旨集, Volume：22nd, First page：516, Last page：517, 10 Sep. 2007
Japanese
J-Global ID：200902277266247846 - 水素結合性ランタノイド錯体の合成と核酸塩基認識能の評価
厚見宙志; 吉本敬太郎; 齋藤伸吾; 大熊盛也; 前田瑞夫; 長崎幸夫
日本分析化学会年会講演要旨集, Volume：56th, First page：378, 05 Sep. 2007
Japanese
J-Global ID：200902261161655880 - キャピラリー電気泳動法における重金属イオンの直接蛍光検出:分子およびシステム設計
竹内慎吾; 鈴木良治; 吉本敬太郎; 齋藤伸吾; 前田瑞夫; 青山政和
日本分析化学会年会講演要旨集, Volume：55th, First page：294, 06 Sep. 2006
Japanese
J-Global ID：200902247333460251
- 改訂6版 分析化学データブック
齋藤伸吾, [Joint editor]
Nov. 2021
Total pages：260
ISBN：4621306529, ASIN：4621306529, EAN：9784621306529 - ベーシックマスター 分析化学
齋藤伸吾(分担執筆), [Joint work]
Aug. 2013
Japanese, Total pages：450
CiNii Books：http://ci.nii.ac.jp/ncid/BB13305748
ISBN：4274214257, ASIN：4274214257, EAN：9784274214257, CiNii Books ID：BB13305748
- DNAアプタマー選抜を科学する
齋藤伸吾
Nov. 2023, [Invited] - CE と機械学習による DNA アプタマー選抜
齋藤伸吾
Oct. 2022, [Invited] - Single-round DNA aptamer selection by means of capillary electrophoresis combined with next generation sequencing: could unique functional aptamers be acquired by sequence data mining?
Shingo Saito
The 2021 International Chemical Congress of Pacific Basin Societies (PacifiChem2021), Dec. 2021, [Invited]
English, Invited oral presentation - キャピラリー電気泳動法によるDNAアプタマー-トロンビン三元複合体の結合サイト、平衡および速度パラメーターの測定
齋藤伸吾
Jul. 2021, [Invited]
Invited oral presentation - CE分離と機械学習によるDNAアプタマー選抜
齋藤伸吾
Jun. 2021, [Invited]
Invited oral presentation - アクチノイドイオン分析への電気泳動法の適用
齋藤伸吾; 原賀智子
May 2021, [Invited]
Invited oral presentation - Multi-Dimensional PAGE for Isolation and Identification of Metalloproteins in Biological Samples
Shingo Saito
Dec. 2020, [Invited]
English, Invited oral presentation - ゲル電気泳動を用いるCu2+配位を介したHA超分子の化学量論組成の推定
中野純佳,斉藤拓巳,半田友衣子,齋藤伸吾
Nov. 2020 - CE による生体分子錯体の結合サイト、平衡および速度パラメーターの測定
齋藤伸吾
Nov. 2020, [Invited] - ゲル電気泳動を用いるCu2+を介したHA超分子の由来が異なることによる超分子化挙動の比較
中野純佳; 松本篤正; 丸茂和樹; 斉藤拓巳; 半田友衣子; 齋藤伸吾
Sep. 2020 - シングルラウンドCE選抜を用いる巨大官能基修飾型新奇DNAアプタマーの創製
太田和希; 渡辺崚; 半田友衣子; 齋藤伸吾
Sep. 2020 - CE による機能性 DNA アプタマーの 1 ラウ ンド選抜
齋藤伸吾
Jun. 2020, [Invited] - ゲル電気泳動分離を用いる地下水および土壌フミン酸のCu2+およびTb3+による超分子化挙動の解析
中野純佳; 風見綸太郎; 丸茂和樹; 斉藤拓巳; 半田友衣子; 齋藤伸吾
May 2020
Others - 蛍光性フェナントロリンジカルボン酸誘導体を用いるネプツニルイオンの特異的な電気泳動分離検出法とその錯体化学特性
山縣和仁; 丸茂和樹; 大内和希; 渋川雅美; 半田友衣子; 原賀智子; 齋藤伸吾
May 2020
Others - 電気泳動法を基盤とした生体試料中微量金属イオンおよび金属タンパク質の分離検出法
齋藤伸吾
Feb. 2020, [Invited] - 1ラウンド電気泳動選抜と大規模配列解析に基づくタンパク質結合型DNAアプタマーの獲得
齋藤伸吾
Jul. 2019, [Invited] - 新規分子と出会うための特異な電気泳動分離
齋藤伸吾
Jul. 2019, [Invited] - 固定化DNAを介して核酸アプタマーを修飾した金ナノ粒子によるトロンビンタンパク質の検出
吉村健; 矢野湧暉; 矢野雄暉; 小川敦司; 前田瑞夫; 朝日剛; 古性均; 齋藤伸吾; 吉本敬太郎; 座古保
May 2019, [Domestic conference]
Japanese, Oral presentation - 金属ー有機構造体[Fe3OF(H2O)2(C9H3O6)2] を固定相とする液体クロマトグラフィーにおける保持機構のスイッチング
吉川真帆; 半田友衣子; 齋藤伸吾; 渋川雅美
May 2019, [Domestic conference]
Japanese, Oral presentation - 表面気泡変調液体クロマトグラフィーによるアセトニトリル水溶液／C18シリカ界面における物質分離機構の解析 －界面における物質分布におよぼす溶媒組成の影響の解明－
中村圭介; 生形竜介; 水野恒; 齋藤伸吾; 渋川雅美
May 2019, [Domestic conference]
Japanese, Oral presentation - 立体構造の異なるランダムDNAサブライブラリーを用いるトロンビン結合型アプタマー選抜
坂本寿樹; 吉本敬太郎; 渋川雅美; 齋藤伸吾
May 2019, [Domestic conference]
Japanese, Oral presentation - アプタマーペアの電気泳動選抜と高性能多価アプタマーの開発
齋藤 伸吾
Mar. 2019, [Invited], [Domestic conference]
Japanese, Invited oral presentation - トロンビン結合型DNAアプタマーの1ラウンドCE選抜
齋藤 伸吾
Dec. 2018, [Invited], [Domestic conference]
Japanese, Invited oral presentation - 立体構造の異なるランダムDNAライブラリーを用いる機能別アプタマー選抜
坂本寿樹; 渋川雅美; 齋藤伸吾
Dec. 2018, [Domestic conference]
Japanese, Poster presentation - 二次元ゲル電気泳動法を用いる金属-フミン酸超分子錯体の凝集機構の解明
丸茂和樹; 斉藤拓巳; 原賀智子; 渋川雅美; 齋藤伸吾
Nov. 2018, [Domestic conference]
Japanese, Poster presentation - アプタマー修飾金ナノ粒子を用いたトロンビン検出におけるアプタマー配列の検討
吉村健; 矢野湧暉; 矢野雄暉; 小川敦司; 前田瑞夫; 古性均; 齋藤伸吾; 吉本敬太郎; 座古保
Nov. 2018, [Domestic conference]
Japanese - キャピラリー電気泳動を用いるDNAアプタマー選抜と機能判別
齋藤 伸吾
Nov. 2018, [Invited], [Domestic conference]
Japanese, Invited oral presentation - メタロタンパク質の分離・同定に特化したホロ/アポ変換二次元PAGE：紅色非硫黄光合成細菌中の銅結合性タンパクの同定
比護大地; 石川順子; Anne Durand; Soufian Ouchane; 渋川雅美; 齋藤伸吾
Nov. 2018, [Domestic conference]
Japanese, Poster presentation - DNAアプタマーペアの電気泳動的選抜と高機能多価アプタマーの合理的設計
齋藤伸吾; 吉本敬太郎
Sep. 2018, [Invited], [Domestic conference]
Invited oral presentation - DNAアプタマー選抜における分離・洗浄工程の重要性：微粒子導入型キャピラリー電気泳動の導入効果
吉本敬太郎; 齋藤伸吾
Sep. 2018, [Invited], [Domestic conference]
Japanese, Invited oral presentation - メタロタンパク質マッピング法としてのMICS-BN-PAGEに関する最新トピック
齋藤 伸吾
Aug. 2018, [Invited], [Domestic conference]
Invited oral presentation - 新しい分子系の発見のための電気泳動的アプローチ
齋藤 伸吾
Sep. 2017, [Invited], [Domestic conference]
Invited oral presentation - キャピラリー電気泳動法を用いる細菌および動物細胞を認識するDNAアプタマーの新規獲得法
齋藤 伸吾
Aug. 2016, [Invited], [Domestic conference]
Japanese, Invited oral presentation - 細胞のキャピラリー電気泳動とDNAアプタマー選抜
齋藤 伸吾
May 2016, [Invited], [Domestic conference]
Invited oral presentation - 発見を目指す電気泳動分離分析
齋藤 伸吾
Jan. 2016, [Invited]
Invited oral presentation - Fluorescence sensing of saccharides using boronic acid functionalized squarylium dyes and their applications to analytical methods
SAITO Shingo
Sep. 2015, [Invited], [International conference]
English, Invited oral presentation - 電気泳動分離場で機能する蛍光プローブ
齋藤 伸吾
Mar. 2015, [Invited], [Domestic conference]
Invited oral presentation - キャピラリー電気泳動を用いる細菌に特異的なDNAアプタマーの高速選抜法の開発
齋藤 伸吾
Nov. 2014, [Invited], [Domestic conference]
Japanese, Invited oral presentation - キャピラリー電気泳動を用いる濃縮―分離―分取法の開発とその応用
齋藤 伸吾
Oct. 2014, [Invited], [Domestic conference]
Invited oral presentation - タンパク質結合型金属イオンのポリアクリルアミドゲル電気泳動によるマッピング
齋藤 伸吾
Oct. 2014, [Invited]
Invited oral presentation - Selection of DNA Aptamer for Bacterial Cell using Polymer-enhanced Capillary Transient Isotachophoresis
SAITO Shingo
Sep. 2014, [Invited], [International conference]
English, Invited oral presentation - 超微量重金属イオンのCE蛍光検出のための試薬および分離システム設計
齋藤 伸吾
Dec. 2009, [Invited]
Invited oral presentation - Superheated Water Ion-Exchange Chromatography: A New Approach to Alteration of Selectivity in Ion-Exchange Separation
2009 - Polyacrylamide gel electrophoresis of trace metal ions bounded to proteins in gel fraction using novel fluorescent probes: Fluorescent detection of trace Fe(III) in transferrin
2009
Poster presentation - 疎水性ナノ空間水が示す無機イオンの分離
2009 - ポリエチレングリコール水溶液におけるCo(II)チオシアナト錯体生成反応 -高分子水溶液中の水の状態とその錯形成反応への影響-
2009
Poster presentation - 水性ニ相抽出を用いた環境水中の低濃度亜鉛の簡易比色分析法の開発
2009
Poster presentation - 八座発蛍光性配位子を用いる金属イオンのCE-LIF分離検出 -カチオン性ポリマーとのイオン会合による配位構造認識-
2009
Poster presentation - C18結合型シリカゲル充填剤内の疎水性微小空間における水の状態変化と分離に及ぼす効果
2009 - PAGEをプラットフォームとするタンパク質結合型金属イオンの検出法の開発：トランスフェリン結合型Fe(III)の検出
2009 - 分離分析システムにおけるランタノイドイオンの高感度検出試薬の開発
齋藤伸吾
2009, [Invited]
Invited oral presentation - 配位子交換反応に基づく固相抽出による合成樹脂中のイミダゾールの分離濃縮
2009
Poster presentation - 水性ニ相抽出法を用いた亜鉛の簡易比色分析法の開発
2009
Poster presentation - 配位構造の異なる金属プローブ錯体のPAGEにおける分離検出特性の調査
2009
Poster presentation - 剛性の高い八座非環状蛍光配位子を用いる金属イオンのキャピラリー電気泳動分離
2009
Poster presentation - C18結合型シリカ充填剤表面に形成する溶媒和液相とその溶質保持に及ぼす影響
2009
Poster presentation - 純水を移動相とする逆相HPLCによる無機イオンの分離：疎水性ナノ空間水の分離機能
2009
Poster presentation - C18結合型シリカ充填剤表面に形成する溶媒和液相とその分離選択性
2009 - Superheated Water Ion-Exchange Chromatography: A New Approach to Alteration of Selectivity in Ion-Exchange Separation
2009 - Polyacrylamide gel electrophoresis of trace metal ions bounded to proteins in gel fraction using novel fluorescent probes: Fluorescent detection of trace Fe(III) in transferrin
2009
Poster presentation - 疎水性ナノ空間水が示す無機イオンの分離
第28回溶媒抽出討論会 講演要旨集, 2009 - ポリエチレングリコール水溶液におけるCo(II)チオシアナト錯体生成反応 -高分子水溶液中の水の状態とその錯形成反応への影響-
日本分析化学会第59年会 講演要旨集, 2009
Poster presentation - 水性ニ相抽出を用いた環境水中の低濃度亜鉛の簡易比色分析法の開発
日本分析化学会第58年会 講演要旨集, 2009
Poster presentation - 八座発蛍光性配位子を用いる金属イオンのCE-LIF分離検出 -カチオン性ポリマーとのイオン会合による配位構造認識-
日本分析化学会第58年会 講演要旨集, 2009
Poster presentation - C18結合型シリカゲル充填剤内の疎水性微小空間における水の状態変化と分離に及ぼす効果
日本分析化学会第58年会 講演要旨集, 2009 - PAGEをプラットフォームとするタンパク質結合型金属イオンの検出法の開発：トランスフェリン結合型Fe(III)の検出
東京コンファレンス2009 講演要旨集, 2009 - 配位子交換反応に基づく固相抽出による合成樹脂中のイミダゾールの分離濃縮
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - 水性ニ相抽出法を用いた亜鉛の簡易比色分析法の開発
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - 配位構造の異なる金属プローブ錯体のPAGEにおける分離検出特性の調査
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - 剛性の高い八座非環状蛍光配位子を用いる金属イオンのキャピラリー電気泳動分離
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - C18結合型シリカ充填剤表面に形成する溶媒和液相とその溶質保持に及ぼす影響
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - 純水を移動相とする逆相HPLCによる無機イオンの分離：疎水性ナノ空間水の分離機能
分離技術会 年会2009 講演要旨集, 2009
Poster presentation - C18結合型シリカ充填剤表面に形成する溶媒和液相とその分離選択性
第70回分析化学討論会 講演要旨集, 2009 - Dissociation Process Kinetics of Metal Chelates. -A Key Factor in the HPLC/HPCE Application-
1998 - Dissociation Process Kinetics of Metal Chelates. -A Key Factor in the HPLC/HPCE Application-
Proceedings of International Trace Analysis Symposium '98 (ITAS '98), 1998 - 二次元ゲル電気泳動法によるランタノイドおよびアクチノイド-フミン酸超分子錯体の凝集反応追跡
丸茂和樹; 斉藤拓巳; 原賀智子; 渋川雅美; 齋藤伸吾
[Domestic conference]
Japanese, Poster presentation
- 2023 - Present
分析化学I（埼玉大学） - 2015 - Present
分析化学II - 2007 - Present
分析化学演習 - 2007 - Present
分析化学特論I - 2007 - Present
応用化学実験IV - 2007 - Present
ナノ化学特論II - Aug. 2024 - Aug. 2024
特別講義5 - 2022 - 2022
最先端生命科学研究特論（分担，集中講義）（千葉工業大学） - 2007 - 2017
応用化学実験II - 2010 - 2013
分析化学III - 2007 - 2012
確率・統計 - 2006
無機分析化学演習 - 2006
無機分析化学 - 2006
無機分析化学実験
■ Research projects
- DNAアプタオミクスに基づく物質暗号化と機能性分子創製
Apr. 2025 - Mar. 2029
Grant amount(Total)：18720000, Direct funding：14400000, Indirect funding：4320000
Grant number：25K01795 - Development of SPF-EEM spectroscopy for non-extraction and non-destructive analysis of natural organic matter
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), 28 Jun. 2024 - 31 Mar. 2028
Hokkaido University
Grant amount(Total)：25870000, Direct funding：19900000, Indirect funding：5970000
Grant number：24K21328 - Innovations in immature water purification process through novel microbial monitoring technologies
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), 01 Apr. 2024 - 31 Mar. 2028
Hokkaido University
Grant amount(Total)：47060000, Direct funding：36200000, Indirect funding：10860000
Grant number：24H00325 - 分離化学とAI解析の融合による分子認識科学としてのDNAアプタオミクス
Apr. 2022 - Mar. 2026
Principal investigator
Grant amount(Total)：17290000, Direct funding：13300000, Indirect funding：3990000
Grant number：22H02104 - Development of pathogen sensors based on the detection of host recognition molecules for the post-PCR era
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), 30 Jun. 2023 - 31 Mar. 2025
Hokkaido University
Grant amount(Total)：6500000, Direct funding：5000000, Indirect funding：1500000
Grant number：23K17767 - 電気泳動法を基盤とするアクチノイドに対する簡易・迅速スクリーニング分析法の開発
01 Apr. 2022 - 31 Mar. 2025
Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
Grant number：22K05180 - Development of new water quality sensing technology for realization of sustainable small-scale water supply systems
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), 05 Apr. 2021 - 31 Mar. 2025
Hokkaido University
Grant amount(Total)：39780000, Direct funding：30600000, Indirect funding：9180000
Grant number：21H04568 - Development and application of innovative monitoring technologies of microorganisms: Challenge to eradication of waterborne infectious diseases in Asia
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), 27 Oct. 2020 - 31 Mar. 2024
Hokkaido University
Grant amount(Total)：18720000, Direct funding：14400000, Indirect funding：4320000
Grant number：20KK0090 - Development of high-performance multi-functional reversed-phase HPLC with the stationary phase containing gas and supercritical fluid
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Apr. 2019 - Mar. 2022
Shibukawa Masami, Saitama University, Coinvestigator
Grant amount(Total)：18070000, Direct funding：13900000, Indirect funding：4170000
This study aims to investigate the function of the gas phase and the supercritical fluid incorporated in porous hydrophobic packing materials in the columns of surface bubble-modulated liquid chromatography and supercritical fluid stationary phase liquid chromatography and develop unprecedented separation technologies. The effects of the structure of the interfacial liquid layer formed on the surface of the hydrophobic packing materials and the chemical structure of the surface of the hydrophobic materials on the separation selectivity were clarified. We also carried out the research with the aim of developing a method for determining volatile organic compounds in an aqueous solution and a high-speed analysis method for inert gases that does not require pretreatment by gas-liquid separation.
Grant number：19H02741 - 機能発現・判別を指向するDNAアプタマーの電気泳動選抜
2019 - 2022
Principal investigator
Competitive research funding - Development of simple, rapid and low-cost onsite simultaneous measurement technique of indicator bacteria based on fluorescence probes
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), 28 Jun. 2019 - 31 Mar. 2021
Satoh Hisashi, Hokkaido University
Grant amount(Total)：6500000, Direct funding：5000000, Indirect funding：1500000
We developed a simple, rapid, and low-cost simultaneous on-site indicator bacteria measurement technique using fluorescent probes. A sample with 40 cfu/L of E. coli could be detected in about 7 hours, and a sample with 4 cfu/L in about 13 hours. The operation is extremely simple: even with untreated sewage, the sample water is placed in a liquid medium or agar medium without any pretreatment, and the fluorescence intensity is automatically measured once every 10 minutes using a microplate reader set at 37°C. Since the number of coliform bacteria can be measured in about three hours for normal treated sewage water, the number of coliform bacteria can be analyzed with high frequency in both time and space, and deterioration of treatment efficiency can be quickly identified. Since the measurement medium is about 0.02 mL, the cost per sample is about 2 Japanese yen.
Grant number：19K21979 - Introduction and evaluation of micro beads-assisted capillary electrophoresis for nucleic acid aptamer selection systems
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Apr. 2018 - Mar. 2021
Yoshimoto Keitaro, The University of Tokyo, Coinvestigator
Grant amount(Total)：17160000, Direct funding：13200000, Indirect funding：3960000
In this research project, the micro-particle-assisted capillary electrophoresis (MACE) system discovered by the applicant was introduced into a nucleic acid aptamer selection experiment (SELEX) system for various target molecules to demonstrate that there is still a large room for growth in the SELEX system, which has been attempted to be improved using a synthetic organic chemistry approach. The objective of this study was to demonstrate that the SELEX method can be significantly improved by using an "analytical chemistry" approach. As a result, we demonstrated that the MACE-SELEX method works on a wide range of targets, including soluble proteins, membrane proteins, and small molecules, and that it is possible to obtain DNA aptamers with high binding affinity.
Grant number：18H02002 - Development of highly sensitive and rapid analytical method for ultratrace actinide ions in radioactive samples by capillary electrophoresis
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), 01 Apr. 2016 - 31 Mar. 2019
Haraga Tomoko; SAITO Shingo; OUCHI Kazuki; SATO Yoshiyuki; HOSHINO Hitoshi; FUJIHARA Takashi; KUROKAWA Hideki; SHIBUKAWA Masami; KAMEO Yutaka; ISHIMORI Ken-ichiro, Japan Atomic Energy Agency
Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
Highly sensitive and rapid analytical method for ultratrace actinide ions in radioactive samples by capillary electrophoresis-laser-induced fluorescence detection (CE-LIF) was developed. We designed emissive probes for the detection of actinide ions with ppt levels using CE-LIF. This method was successfully applied to real radioactive liquid samples collected from nuclear facilities, including the Fukushima Daiichi Nuclear Power Plant.
Grant number：16K17926 - Development and functionalization of hybrid separation systems containing nano-bubbles and nano-supercritical fluid
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Apr. 2016 - Mar. 2019
Shibukawa Masami, Saitama University, Coinvestigator
Grant amount(Total)：17940000, Direct funding：13800000, Indirect funding：4140000
We developed surface-bubble-modulated liquid chromatography (SBMLC), which has a hybrid stationary phase consisting of gas phase as well as the hydrophobic material and the water/hydrophobe interface and showed that this chromatographic method can be performed with the columns packed with various types of hydrophobic porous materials. We determined the distribution coefficients of various organic compounds referring to accumulations onto the water/alkyl chain interface and into the alkyl chain layer from the bulk water by SBMLC. The experimental data provide a picture of the spatial distribution of organic molecules in alkyl bonded silica particles exposed to water. We also developed a liquid chromatography with supercritical fluid stationary phase, which has the hybrid stationary phase consisting of the supercritical carbon dioxide phase, the interfacial liquid phase and the hydrophobic moiety.
Grant number：16H04161 - 高い細胞認識能を有するDNAアプタマーの高速な電気泳動選抜法の開発
Apr. 2016 - Mar. 2019
Principal investigator
Competitive research funding - 多次元電気泳動法を用いるアクチノイドイオンの腐植物質による保持挙動の解明
2017 - 2018
Principal investigator
Competitive research funding - Development of the hybrid separation system containing nano-bubbles as key components
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Apr. 2013 - Mar. 2016
SHIBUKAWA Masami; SAITO SHINGO, Saitama University, Coinvestigator
Grant amount(Total)：18460000, Direct funding：14200000, Indirect funding：4260000
A new chromatographic method termed surface-bubble-modulated liquid chromatography, that has a hybrid separation medium incorporated with surface nanobubbles, was developed. Nanobubbles can be fixed at the interface between water and a hydrophobic material by delivering water into a dry column packed with a nanoporous material. The incorporation of a gas phase leads to the formation of the hybrid separation system consisting of the gas phase, hydrophobic moieties, and the water/hydrophobic interface or the interfacial water. One can change the volume of the gas phase by pressure applied to the column, which in turn alters the area of water/hydrophobic interface, while the amount of the hydrophobic moiety remains constant. Therefore this strategy provides a novel technique not only for manipulating the separation selectivity by pressure but also for elucidating the mechanism of accumulation or retention of solute compounds in aqueous solutions by a hydrophobic material.
Grant number：25288062 - 金属タンパク質の分布測定を指向した多次元ポリアクリルアミドゲル電気泳動法の開発
Apr. 2013 - Mar. 2016
Principal investigator
Competitive research funding - ゲル電気泳動法を基盤とする環境中腐食物質-重金属錯体のスペシエーション
2015 - 2015
Principal investigator
Competitive research funding - Multi-dimension polyacrylamide gel electrophoresis for detection of metal ions bound to metalloproteins
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Apr. 2011 - Mar. 2013
SAITO Shingo, Saitama University, Principal investigator
A polyacrylamide gel electrophoresis (PAGE)-based method has been developed, consisting of two gel electrophoresis, to obtain distribution of protein-bound metal ions in biological samples. The former is blue-native (BN) PAGE to separate proteins without dissociation during migration. The other is a PAGE for the separation and detection of protein-bound metal ions in small volume samples with high sensitivity in the ppt range using fluorescence metal probe. The probes have a chelating moiety (multidentate polyaminocarboxylate), a fluorophore (fluorescein) and a spacer (aminobenzyl group) to form emissive and kinetic inert complexes with metal ions. Some metal ions, Mg^<2+>, Cu^<2+>, Fe^<2+>/^<3+>, Ni^<2+>, Co^<2+>, Mn^<2+>, Cd^<2+> and Hg^<2+>, were successfully detected by this new PAGE. After separation of the proteins by BN-PAGE, the metal ions in the gel fractions were eluted, followed by derivatization of copper ions into the metal probe complexes to be separated and determined by fluorescence detection in the second PAGE. In this PAGE-based method, the copper ions bound to ceruloplasmin and superoxidedismutase, and iron ions bound to transferrin and hemopexin were quantitatively determined.
Competitive research funding, Grant number：23750077 - アクチノイドイオン適合型キャピラリー電気泳動用蛍光プローブおよびプローブ錯体の精密分離検出技術の開発
2010 - 2012
Principal investigator
Competitive research funding - Electrophoresis for metal ion-protein mapping
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Apr. 2009 - Mar. 2011
SAITO Shingo, Saitama University, Principal investigator
Novel fluorescent probes for detecting metal ions working in capillary electrophoresi-laser-induced fluorescence detection (CE-LIF) and polyacrylamide gel electrophoresis (PAGE) have been developed. The detection limits for several transition metal ions of tens pM were successfully obtained. In CE-LIF, the addition of cationic polymer provided high resolution among metal-probe complexes with new separation principle. The new separation mode was named as "Ion association complex separation mode." In PAGE, Fe and Cu ions which existed in the gel fractions of transferrin and ceruloplasmin were successfully determined.
Competitive research funding, Grant number：21750072 - Development of reaction-selective separation systems with superheated water and analysis of chemical reactions in aqueous solutions at elevated temperature
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), 2008 - 2011
SHIBUKAWA Masami; SAITO Shingo, Saitama University, Coinvestigator
Grant amount(Total)：20020000, Direct funding：15400000, Indirect funding：4620000
Ion-exchange chromatography with superheated water, i.e., liquid water under pressure at temperatures above 100 °C (SW-IEC) was developed and the mechanism of ion-exchange separation processes was investigated using SW-IEC. Based on the results obtained, it was demonstrated that SW-IEC is a powerful tool for changing separation selectivity for ionic solutes drastically by controlling temperature. The states and separation function of the interfacial water on hydrophobic surfaces were clarified and an HPLC method was presented for determination of equilibrium constants and kinetic constants of chemical reactions proceeding on the hydrophobic surfaces as well as in the bulk solution phases. Furthermore redox derivatization two-dimensional HPLC system was developed using catalytic redox activity of porous graphitic carbon and it was shown that this separation technique is very useful for selective separation and determination of trace amounts of reducible and oxidizable compounds.
Grant number：20350034 - Fluorescent detection of emissive heavy metal complexes at sub ppt levels with capillary electrophoresis using dynamic ternary complex equilibrium
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Apr. 2006 - Mar. 2009
SAITO Shingo, Principal investigator
Competitive research funding, Grant number：18750057 - ゲル電気泳動をプラットフォームとするメタローム解析技術の開発
2008 - 2009
Principal investigator
Competitive research funding - 簡易なメタローム解析技術の開発
2007 - 2007
Principal investigator
Competitive research funding - 血清中ｐｐｔレベル重金属イオンの一斉検出システムのキット化
2005 - 2005
Principal investigator
Competitive research funding - Development of the Methodology for Dissociation Kinetic Analysis of Biomolecular Complexes with Capillary Electrophotetic Reactor
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 2003 - 2004
TAKAHASHI Toru; IKI Nobuhiko; SAITOH Shingo, Tohoku University
Grant amount(Total)：13100000, Direct funding：13100000
A microchip capillary electrophoretic reactor (MCER), a practical and powerful tool for dissociation kinetic analysis of metal complexes, has been proposed. In the dissociation kinetic analysis of metal complexes using conventional batch reaction techniques, owing to the difficulty in dividing between reactant (metal complex) and products (metal ion and free ligand), the direct measurement of the dissociation kinetics had not been achieved. On the contrary, MCER revolutionized the measurement of the dissociation rate constant of metal complexes since its accomplishment of the direct measurement. The basic idea of a MCER was provoked by the concentration jump character of the sharp band profiles in microchip capillary electrophoresis (MCE) ; i.e. metal complexes during the MCE separation processes, in which there had been no ligand in the electrophoretic buffer solution, were exposed to overwhelming force to dissociation caused by a steep concentration jump of the ligand. The time depending dissociation reaction profile of metal complex of Ce^<3+> with a polyaminocarboxylic ligand, 8-amino-2-[(2-amino-5-methylphenoxyl)methyl]-6-methoxyquinoline-N,N,N',N',-tetraacetic acid ([CeL]^-) was described using both internal and external standard. The solvolytic dissociation rate constant of [CeL]^-have been determined directly as 6.2 X 10^<-3> s^<-1> at 303 K with MCER.
Capillary electrophoretic reactor (CER) was applied to the determination of dissociation reaction rate constant of bio-molecular complexes. Both an immunocomplex of anti digoxin IgG with digoxin and a complex double stranded DNA with ethidium bromide were chosen as analytical model compounds. Unfortunately, determination of dissociation reaction rate constants could not be achieved with CER in both systems, since their properties of small electrophoretic mobility and lower kinetically inertness.
Grant number：15350039
- アプタマーの選抜方法
Patent right
Patent/Registration no：特許6781883 - アメリシウム及びキュリウム測定用蛍光プローブ、並びにアメリシウム及びキュリウムの分雛及び定量分析方法
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Patent/Registration no：特許6332669 - 核酸アプタマー
Patent right - MEASUREMENT METHOD OF EQUILIBRIUM DISSOCIATION CONSTANT AND ALLOSTERIC EFFECT
Patent right - キャピラリー等速電気泳動法を用いる複数回大容量注入－濃縮－分離－分取精製法
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Patent/Registration no：特許6028997 - 蛍光性ウラン錯体を形成する化合物、その合成方法、ウラン測定用蛍光プローグ及びウランの測定方法
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Patent/Registration no：特許5834274 - COMPPOUND FOR FORMING FLUORESCENT URANIUM COMPLEX, METHOD FOR SYNTHESIZING THEREOF, FLUORESCENT PROBE FOR DETECTING URANIUM AND METHOD FOR ANALYZING URANIUM
Tomoko Haraga; Shingo Saito; Yoshiyuki Sato, Patent right - 試料中の金属の測定方法
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Patent/Registration no：特許5145511 - 金属測定用蛍光プローブ
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Patent/Registration no：特許5055595 - 流れ分離分析法による金属測定に使用する試薬及び測定方法
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Patent/Registration no：特許4571379
- Single-round DNA aptamer selection by combined use of CE and next generation sequencing
Other than myself, https://analyticalscience.wiley.com/do/10.1002/was.0110292, Jun. 2021, [Internet] - 合成DNA薬 治療効果20倍
Other than myself, 18 Oct. 2018, [Paper] - 15種のアミノ酸，溶離液１つで分解 埼玉大が精製技術
Other than myself, 24 May 2018, [Paper] - 堀場賞受賞
18 Oct. 2013, [Paper] - 堀場賞受賞
Other than myself, 18 Oct. 2013, [Paper] - All in the sugar coating: Developing a CE method for detecting pathogenic bacteria
Other than myself, SeparationNOW.com, 19 Mar. 2012, [Internet] - アルミニウムブランク値の化学的提言
Other than myself, 2006, [Pr]