業績情報

• キャピラリー電気泳動1 ラウンド選抜法によるDNA アプタマー配列データのAI解析　　　　　　　　　　　　　　　
  齋藤伸吾
  ぶんせき, 巻：11, 開始ページ：404, 終了ページ：412, 2024年11月, ［査読有り］, ［筆頭著者, 責任著者］
• Cooperativity between coordinative unsaturated Fe(iii) and aryl-π electrons in MIL-100(Fe) for adsorption of small molecules
  Yuiko Tasaki-Handa; Kazuki Shibuya; Shingo Saito
  New Journal of Chemistry, 巻：48, 号：24, 開始ページ：11107, 終了ページ：11113, 2024年05月, ［査読有り］
  Coordinatively unsaturated sites and aryl-π electrons coorperatively affect the adsorption of aromatics with polar groups.
  Royal Society of Chemistry (RSC), 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/d4nj01385a
  DOI ID：10.1039/d4nj01385a, ISSN：1144-0546, eISSN：1369-9261
• Multistep pH-peak-focusing liquid chromatography with a hydrophilic polymer gel column for separation of rare earth elements　　　　　　　　　　　　　　　
  Masami Shibukawa; Yosuke Onoyama; Yuiko Handa-Tasaki; Shingo Saito
  Journal of Chromatography A, 巻：1721, 開始ページ：464829, 終了ページ：464829, 2024年04月, ［査読有り］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2024.464829
  DOI ID：10.1016/j.chroma.2024.464829, ISSN：0021-9673
• Determination of 90Sr in highly radioactive aqueous samples via conversion to a kinetically stable 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex followed by concentration–separation–fractionation based on capillary electrophoresis–liquid scintillation　　　　　　　　　　　　　　　
  Kazuki Ouchi; Tomoko Haraga; Kazuki Hirose; Yuika Kurosawa; Yoshiyuki Sato; Masami Shibukawa; Shingo Saito
  Analytica Chimica Acta, 巻：1298, 開始ページ：342399, 終了ページ：342399, 2024年04月, ［査読有り］, ［最終著者, 責任著者］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.aca.2024.342399
  DOI ID：10.1016/j.aca.2024.342399, ISSN：0003-2670
• Structure of polyethylene glycol/water mixtures as a separation medium of aqueous two-phase extraction systems studied by equilibrium analysis of thiocyanato complexation of Cobalt(II)　　　　　　　　　　　　　　　
  Masami Shibukawa; Tomoyasu Hirasawa; Shingo Saito
  Polymer, 開始ページ：126658, 終了ページ：126658, 2024年01月, ［査読有り］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.polymer.2023.126658
  DOI ID：10.1016/j.polymer.2023.126658, ISSN：0032-3861
• Holo/apo conversion two-dimensional urea PAGE for speciation of Fe3+-bound transferrin in serum
  Shingo Saito; Junko Ishikawa; Moe Ono; Yuiko Tasaki-Handa; Masami Shibukawa
  Analytical Sciences, 2023年11月, ［査読有り］, ［筆頭著者, 責任著者］
  Springer Science and Business Media LLC, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s44211-023-00460-z
  DOI ID：10.1007/s44211-023-00460-z, ISSN：0910-6340, eISSN：1348-2246
• キャピラリー電気泳動法　　　　　　　　　　　　　　　
  齋藤伸吾
  ぶんせき, 号：10, 開始ページ：390, 終了ページ：397, 2023年10月, ［査読有り］, ［招待有り］, ［筆頭著者, 責任著者］
• Americium(III)/Curium(III) Complete Separation and Sensitive Fluorescence Detection by Capillary and Gel Electrophoresis Using Emissive Hexadentate/Octadentate Polyaminocarboxylate Ligands
  Shingo Saito; Tomoko Haraga; Kazuki Marumo; Yoshiyuki Sato; Yuta Nakano; Yuiko Tasaki-Handa; Masami Shibukawa
  Bulletin of the Chemical Society of Japan, 巻：96, 号：3, 開始ページ：223, 終了ページ：225, 2023年03月, ［査読有り］, ［筆頭著者, 責任著者］
  The Chemical Society of Japan, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1246/bcsj.20220346
  DOI ID：10.1246/bcsj.20220346, ISSN：0009-2673, eISSN：1348-0634
• Unusually Kinetically Inert Monocationic Neptunyl Complex with a Fluorescein-Modified 1,10-Phenanthroline-2,9-dicarboxylate Ligand: Specific Separation and Detection in Gel Electrophoresis
  Kazuhito Yamagata; Kazuki Ouchi; Kazuki Marumo; Yuiko Tasaki-Handa; Tomoko Haraga; Shingo Saito
  Inorganic Chemistry, 巻：62, 号：2, 開始ページ：730, 終了ページ：738, 2023年01月, ［査読有り］, ［最終著者, 責任著者］
  American Chemical Society (ACS), 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.inorgchem.2c02908
  DOI ID：10.1021/acs.inorgchem.2c02908, ISSN：0020-1669, eISSN：1520-510X
• Lanthanide ion exchange modulated via crystalline phase transition of a mixed-metal coordination polymer based on bis(4-nitrophenyl) phosphate　　　　　　　　　　　　　　　
  Yuiko Tasaki-Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
  Inorganic Chemistry Communications, 巻：140, 開始ページ：109428, 終了ページ：109428, 2022年06月, ［査読有り］, ［最終著者］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.inoche.2022.109428
  DOI ID：10.1016/j.inoche.2022.109428, ISSN：1387-7003
• Characterization of the Interfacial Liquid Layer Formed on Hydrophobic Packing Material Surfaces by Liquid Chromatographic Analysis of the Distribution of Ions and Molecules
  Masami Shibukawa; Hiroki Okutsu; Shingo Saito
  ACS Omega, 2022年04月, ［査読有り］
  American Chemical Society (ACS), 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acsomega.2c01184
  DOI ID：10.1021/acsomega.2c01184, ISSN：2470-1343, eISSN：2470-1343
• Separation approaches towards understanding supramolecular aggregate formation of humic acid
  Shingo Saito
  Analytical Sciences, 巻：38, 号：2, 開始ページ：233, 終了ページ：234, 2022年02月, ［査読有り］, ［筆頭著者, 最終著者, 責任著者］
  Springer Science and Business Media LLC, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s44211-021-00020-3
  DOI ID：10.1007/s44211-021-00020-3, ISSN：0910-6340, eISSN：1348-2246
• A chromatographic approach for studying the adsorption of polar small molecules on tetrabutylammonium bromide semiclathrate hydrate
  Yuiko Tasaki-Handa; Atsuto Iwashita; Masami Shibukawa; Shingo Saito
  Analytical Sciences, 巻：38, 号：1, 開始ページ：85, 終了ページ：90, 2022年01月, ［査読有り］, ［最終著者］
  Springer Science and Business Media LLC, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.21p182
  DOI ID：10.2116/analsci.21p182, ISSN：0910-6340, eISSN：1348-2246
• Quantitation of Trace Lanthanide and Actinide Ions in Radioactive Samples by Capillary Electrophoresis-Laser-Induced Fluorescence Detection
  Tomoko HARAGA; Shingo SAITO
  BUNSEKI KAGAKU, 巻：70, 号：12, 開始ページ：671, 終了ページ：679, 2021年12月, ［査読有り］, ［招待有り］, ［最終著者, 責任著者］
  Japan Society for Analytical Chemistry, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.70.671
  DOI ID：10.2116/bunsekikagaku.70.671, ISSN：0525-1931
• Alkali Metal Ion-exchange in a Metal-Organic Framework Based on Lanthanum and 1,4-Phenylenebis(methylidyne)tetrakis(phosphonic acid)
  Yuiko Tasaki-Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
  Analytical Sciences, 巻：37, 号：12, 開始ページ：1835, 終了ページ：1837, 2021年12月, ［査読有り］, ［最終著者］
  Springer Science and Business Media LLC, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.21n022
  DOI ID：10.2116/analsci.21n022, ISSN：0910-6340, eISSN：1348-2246
• Stoichiometry between Humate Unit Molecules and Metal Ions in Supramolecular Assembly Induced by Cu2+ and Tb3+ Measured by Gel Electrophoresis Techniques
  Sumika Nakano; Kazuki Marumo; Rintaro Kazami; Takumi Saito; Tomoko Haraga; Yuiko Tasaki-Handa; Shingo Saito
  Environmental Science & Technology, 巻：55, 号：22, 開始ページ：15172, 終了ページ：15180, 2021年11月, ［査読有り］, ［最終著者, 責任著者］
  American Chemical Society (ACS), 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.est.1c03993
  DOI ID：10.1021/acs.est.1c03993, ISSN：0013-936X, eISSN：1520-5851
• Single‐Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein‐Binding DNA Aptamers　　　　　　　　　　　　　　　
  Shingo Saito; Toshiki Sakamoto; Naoki Tanaka; Ryo Watanabe; Takuya Kamimura; Kazuki Ota; Kathryn R. Riley; Keitaro Yoshimoto; Yuiko Tasaki‐Handa; Masami Shibukawa
  Chemistry – A European Journal, 巻：27, 号：39, 開始ページ：10058, 終了ページ：10067, 2021年07月, ［査読有り］, ［筆頭著者, 責任著者］
  In DNA aptamer selection, existing methods do not discriminate aptamer sequences based on their binding affinity and function and the reproducibility of the selection is often poor, even for the selection of well-known aptamers like those that bind the commonly used model protein thrombin. In the present study, a novel single-round selection method (SR-CE selection) was developed by combining capillary electrophoresis (CE) with next generation sequencing. Using SR-CE selection, a successful semi-quantitative and semi-comprehensive aptamer selection for thrombin was demonstrated with high reproducibility for the first time. Selection rules based on dissociation equilibria and kinetics were devised to obtain families of analogous sequences. Selected sequences of the same family were shown to bind thrombin with high affinity. Furthermore, data acquired from SR-CE selection was mined by creating sub-libraries that were categorized by the functionality of the aptamers (e. g., pre-organized aptamers versus structure-induced aptamers). Using this approach, a novel fluorescent molecular recognition sensor for thrombin with nanomolar detection limits was discovered. Thus, in this proof-of-concept report, we have demonstrated the potential of a "DNA Aptaomics" approach to systematically design functional aptamers as well as to obtain high affinity aptamers.
  Wiley, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/chem.202100177
  DOI ID：10.1002/chem.202100177, ISSN：0947-6539, eISSN：1521-3765, Web of Science ID：WOS:000659714000001
• Effect of coexisting alkali metal ions on the variation in the coordination mode of 1,4-phenylenbis(methylidyne)tetrakis(phosphonic acid) in a lanthanum(III) metal–organic framework　　　　　　　　　　　　　　　
  Yuiko Tasaki-Handa; Masami Shibukawa; Shingo Saito
  Inorganic Chemistry Communications, 巻：128, 開始ページ：108560, 終了ページ：108560, 2021年06月, ［査読有り］, ［最終著者］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.inoche.2021.108560
  DOI ID：10.1016/j.inoche.2021.108560, ISSN：1387-7003
• SELEX-based DNA Aptamer Selection: A Perspective from the Advancement of Separation Techniques
  Shingo SAITO
  Analytical Sciences, 巻：37, 号：1, 開始ページ：17, 終了ページ：26, 2021年01月, ［査読有り］, ［筆頭著者, 責任著者］
  Japan Society for Analytical Chemistry, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.20sar18
  DOI ID：10.2116/analsci.20sar18, ISSN：0910-6340, eISSN：1348-2246
• Intrinsic difference between phenyl hexyl- and octadecyl-bonded silicas in the solute retention selectivity in reversed-phase liquid chromatography with aqueous mobile phase　　　　　　　　　　　　　　　
  Keisuke Nakamura; Shingo Saito; Masami Shibukawa
  Journal of Chromatography A, 巻：1628, 開始ページ：461450, 終了ページ：461450, 2020年09月, ［査読有り］
  Elsevier BV, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2020.461450
  DOI ID：10.1016/j.chroma.2020.461450, ISSN：0021-9673
• Transmetalation in a Ce(III)‐phosphoester Crystalline Coordination Polymer with an Exceptionally High Selectivity for Yb(III) and Lu(III)　　　　　　　　　　　　　　　
  Yuiko Tasaki‐Handa; Shiori Tsuda; Masami Shibukawa; Shingo Saito
  Chemistry – An Asian Journal, 巻：15, 号：17, 開始ページ：2653, 終了ページ：2659, 2020年07月, ［査読有り］, ［国際誌］
  A novel crystalline coordination polymer containing Ce3+ and bis(4-nitrophenyl) phosphate (L), CeL3 , was synthesized and its unique transmetalation selectivities toward Yb3+ and Lu3+ in the lanthanide series were evaluated. The relatively large difference in transmetalation selectivity between the neighboring Tm3+ and Yb3+ species is noteworthy because the reactivities of heavy lanthanides are generally considerably similar. The structural strain of the polymeric framework is likely responsible for this unusual trend. Powder X-ray diffraction analysis indicated that, in the cases of only Yb3+ and Lu3+ , large differences in their ionic sizes compared to that of Ce3+ in the parent framework may induce a structural strain after solid solution formation, while cleavage of the relatively weak Ce-O bond allows the formation of new Yb-O and Lu-O bonds with the incoming Yb3+ and Lu3+ , respectively. Structural phase transitions likely caused by the heterogeneous nucleation of the Yb- (or Lu-) type phase were also observed.
  Wiley, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/asia.202000502
  DOI ID：10.1002/asia.202000502, ISSN：1861-4728, eISSN：1861-471X, PubMed ID：32502320
• Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis
  Tomoko Haraga; Hiroto Tsujimura; Saori Miyauchi; Takuya Kamimura; Masami Shibukawa; Shingo Saito
  ELECTROPHORESIS, 巻：41, 号：13-14, 開始ページ：1152, 終了ページ：1159, 2020年07月, ［査読有り］, ［最終著者, 責任著者］
  Wiley, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/elps.201900399
  DOI ID：10.1002/elps.201900399, ISSN：0173-0835, eISSN：1522-2683
• Advanced Gel Electrophoresis Techniques Reveal Heterogeneity of Humic Acids Based on Molecular Weight Distributions of Kinetically Inert Cu2+–Humate Complexes　　　　　　　　　　　　　　　
  Kazuki Marumo; Atsumasa Matsumoto; Sumika Nakano; Masami Shibukawa; Takumi Saito; Tomoko Haraga; Shingo Saito
  Environmental Science & Technology, 巻：53, 号：24, 開始ページ：14507, 終了ページ：14515, 2019年11月, ［査読有り］, ［責任著者］
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.est.9b05169
  DOI ID：10.1021/acs.est.9b05169
• Two-Dimensional Polyacrylamide Gel Electrophoresis for Metalloprotein Analysis Based on Differential Chemical Structure Recognition by CBB Dye　　　　　　　　　　　　　　　
  Junko Ishikawa; Akinori Maeshima; Allyson Mellinger; Anne Durand; Marie-Line Bourbon; Daichi Higo; Christa L Colyer; Masami Shibukawa; Soufian Ouchane; Shingo Saito
  Scientific Reports, 巻：9, 号：1, 開始ページ：10566, 2019年07月, ［査読有り］, ［責任著者］
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1038/s41598-019-46955-6
  DOI ID：10.1038/s41598-019-46955-6
• Rapidly Neutralizable and Highly Anticoagulant Thrombin-Binding DNA Aptamer Discovered by MACE SELEX　　　　　　　　　　　　　　　
  Koji Wakui; Toru Yoshitomi; Akane Yamaguchi; Maho Tsuchida; Shingo Saito; Masami Shibukawa; Hitoshi Furusho; Keitaro Yoshimoto
  Molecular Therapy-Nucleic Acids, 巻：16, 開始ページ：348, 終了ページ：359, 2019年06月, ［査読有り］
  We present a rapidly neutralizable and highly anticoagulant thrombin-binding aptamer with a short toehold sequence, originally discovered by systematic evolution of ligands by exponential enrichment (SELEX) with microbead-assisted capillary electrophoresis (MACE). MACE is a novel CE-partitioning method for SELEX and able to separate aptamers from a library of unbound nucleic acids, where the aptamer and target complexes can be detected reliably and partitioned with high purity even in the first selection cycle. Three selection rounds of MACE-SELEX discovered several TBAs with a nanomolar affinity (K-d = 4.5-8.2 nM) that surpasses previously reported TBAs such as HD1, HD22, and NU172 (K-d = 118, 13, and 12 nM, respectively). One of the obtained aptamers, M08, showed a 10- to 20-fold longer prolonged clotting time than other anticoagulant TBAs, such as HD1, NU172, RE31, and RA36. Analyses of the aptamer and thrombin complexes using both bare and coated capillaries suggested that a large number of efficient aptamers are missed in conventional CE-SELEX because of increased interaction between the complex and the capillary. In addition, the toehold-mediated rapid antidote was designed for safe administration. The efficient aptamer and antidote system developed in the present study could serve as a new candidate for anticoagulant therapy.
  CELL PRESS, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.omtn.2019.03.002
  DOI ID：10.1016/j.omtn.2019.03.002, ISSN：2162-2531, Web of Science ID：WOS:000470250900031
• Facilitated Dehydration of Rb+ Ions in Cation‐Exchange Resin when Surrounded by Cs+ Ions: A Marked Phenomenon in Superheated Water
  M. Harada; T. Okada; K. Nakamura; S. Saito; M. Shibukawa
  Chemistry Select, 巻：4, 号：16, 開始ページ：4718, 終了ページ：4725, 2019年04月, ［査読有り］
  Wiley, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/slct.201900388
  DOI ID：10.1002/slct.201900388, ISSN：2365-6549, eISSN：2365-6549
• Excess adsorption of acetonitrile and water on MIL-100 (Fe) and its potential application in mixed-mode chromatography　　　　　　　　　　　　　　　
  Yuiko Tasaki-Handa; Maho Yoshikawa; Shingo Saito; Masami Shibukawa
  New Journal of Chemistry, 巻：43, 号：42, 開始ページ：16566, 終了ページ：16571, 2019年, ［査読有り］
  The excess adsorption isotherm of acetonitrile (ACN) on a metal-organic framework constructed from Fe(iii) and 1,3,5-benzenetricarboxylic acid (MIL-100(Fe)) in ACN/water solution was measured and converted to adsorption isotherms of ACN and water. Excess ACN was adsorbed on MIL-100(Fe) when the volume fraction of ACN (x(A)) was smaller than similar to 0.55, while excess water was adsorbed when x(A) >= 0.55. Water should adsorb on Fe(iii), while ACN has hydrophobic interactions with the aromatic rings of 1,3,5-benzenetricarboxylic acid. The adsorption isotherms indicate that water and ACN adsorption were comparable, indicating that MIL-100(Fe) may be applicable as the stationary phase in mixed-mode liquid chromatography. Investigation of solute retention revealed that the order of solute retention is switched depending on x(A). At x(A) <= 0.7, solute retention may be dominated by hydrophobic interactions between the solute and the aromatic rings of the MIL-100(Fe) framework. At x(A) >= 0.7, solute retention cannot be explained simply by the normal-phase or reversed-phase mode. Interaction with Fe(iii) should be taken into account in addition to hydrophobic interactions. Therefore, MIL-100(Fe) is a potential candidate for the stationary phase in mixed mode liquid chromatography.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c9nj03981c
  DOI ID：10.1039/c9nj03981c, ISSN：1144-0546, eISSN：1369-9261, Web of Science ID：WOS:000493080000016
• Carbon Dot-Mediated Capillary Electrophoresis Separations of Metallated and Demetallated Forms of Transferrin Protein　　　　　　　　　　　　　　　
  Leona R. Sirkisoon; Honest C. Makamba; Shingo Saito; Christa L. Colyer
  Molecules, 巻：24, 号：10, 開始ページ：1916, 2019年01月, ［査読有り］
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.3390/molecules24101916
  DOI ID：10.3390/molecules24101916
• Effect of Acetonitrile on the Solute Distribution at the Heterogeneous Interface Region between Water and Hydrocarbonaceous Silica Revealed by Surface-Bubble-Modulated Liquid Chromatography　　　　　　　　　　　　　　　
  Keisuke Nakamura; Ryusuke Ubukata; Hisashi Mizuno; Shingo Saito; Masami Shibukawa
  Journal of Physical Chemistry C, 巻：122, 号：50, 開始ページ：28674, 終了ページ：28683, 2018年11月, ［査読有り］
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.jpcc.8b08435
  DOI ID：10.1021/acs.jpcc.8b08435
• Adsorption at the Water/Hydrophobe Interface versus Partition into the Interior of the Hydrophobe: Quantitative Evaluation of the Solute Retention Selectivity at the Water/Hydrocarbon Interface (vol 122, pg 4409, 2018)　　　　　　　　　　　　　　　
  Nakamura Keisuke; Saito Shingo; Shibukawa Masami
  JOURNAL OF PHYSICAL CHEMISTRY C, 巻：122, 号：38, 開始ページ：22223, 2018年09月, ［査読有り］
  DOI：https://doi.org/10.1021/acs.jpcc.8b08438
  DOI ID：10.1021/acs.jpcc.8b08438, ISSN：1932-7447, Web of Science ID：WOS:000446141900058
• Identification of new classes of thrombin-binding aptamers by microbeads-assisted capillary electrophoresis (MACE) SELEX　　　　　　　　　　　　　　　
  Wakui Koji; Yoshitomi Toru; Tsuchida Maho; Saito Shingo; Shibukawa Masami; Furusho Hitoshi; Yoshimoto Keitaro
  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 巻：255, 2018年03月, ［査読有り］
  AMER CHEMICAL SOC, 英語
  ISSN：0065-7727, Web of Science ID：WOS:000435537700330
• Adsorption at the Water/Hydrophobe Interface versus Partition into the Interior of the Hydrophobe: Quantitative Evaluation of the Solute Retention Selectivity at the Water/Hydrocarbon Interface　　　　　　　　　　　　　　　
  Keisuke Nakamura; Shingo Saito; Masami Shibukawa
  Journal of Physical Chemistry C, 巻：122, 号：8, 開始ページ：4409, 終了ページ：4418, 2018年03月, ［査読有り］
  Molecular level understanding of the chemistry at the water/hydrophobe interface is crucial to chemical separation processes in aqueous media, such as reversed-phase liquid chromatography and solid-phase extraction. However, direct observation of the behavior of molecules and ions at the interface in reversed-phase separation systems still remains a major challenge, and probing techniques that provide the spatial information of the distribution of molecules and ions are required. In this paper, the molecular distribution between the aqueous solution and alkyl-bonded silica particles is studied by surface-bubble-modulated liquid chromatography (SBMLC). We determine the distribution coefficients of various organic compounds referring to their accumulations onto the water/alkyl chain interface and into the alkyl chain layer from the bulk water by SBMLC. The bulk water-to-alkyl chain layer distribution coefficient is corrected for the contribution of the end-capped silica surface to the solute retention using end-capped C8- and C18-bonded silica columns. The experimental data provide a picture of the spatial distribution of organic molecules in alkyl-bonded silica particles exposed to water. It has been revealed that the water/alkyl chain interface exhibits an accumulation selectivity for organic compounds which is quite different from that of the interior of the alkyl chain layer and the overall separation selectivity of the reversed-phase systems is determined by the relative sizes of the aqueous/hydrophobe interface and the hydrophobe.
  American Chemical Society, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.jpcc.7b12413
  DOI ID：10.1021/acs.jpcc.7b12413, ISSN：1932-7455, SCOPUS ID：85042746291, Web of Science ID：WOS:000426802500035
• Determination of Free Magnesium Oxide in Steelmaking Slags by Microwave-Assisted-Hydration/Thermogravimetry　　　　　　　　　　　　　　　
  Misa Kato; Kenta Tsukagoshi; Michihiro Aimoto; Shingo Saito; Masami Shibukawa
  ISIJ International, 巻：58, 号：10, 開始ページ：1832, 終了ページ：1837, 2018年, ［査読有り］
  英語, 研究論文（学術雑誌）
• Partition/Ion-exclusion chromatographic ion stacking for the analysis of trace anions in water and salt samples by ion chromatography　　　　　　　　　　　　　　　
  Fouzia Akter; Shingo Saito; Yuiko Tasaki-Handa; Masami Shibukawa
  Analytical Sciences, 巻：34, 号：3, 開始ページ：369, 終了ページ：373, 2018年, ［査読有り］
  A new analytical methodology for a simple and efficient on-line preconcentration of trace inorganic anions in water and salt samples prior to ion chromatographic determination is proposed. The preconcentration method is based on partition/ ion-exclusion chromatographic ion stacking (PIEC ion stacking) with a hydrophilic polymer gel column containing a small amount of fixed anionic charges. The developed on-line PIEC ion stacking-ion chromatography method was validated by recovery experiments for the determination of nitrate in tap water in terms of both accuracy and precision, and the results showed the reliability of the method. The method proposed was also successfully applied to the determination of trace impurity nitrite and nitrate in reagent-grade salts of sodium sulfate. A low background level can be achieved since pure water is used as the eluant for the PIEC ion stacking. It is possible to reach sensitive detection at sub-μg L-1 levels by on-line PIEC ion stacking-ion chromatography.
  Japan Society for Analytical Chemistry, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.34.369
  DOI ID：10.2116/analsci.34.369, ISSN：1348-2246, SCOPUS ID：85043362628, Web of Science ID：WOS:000429634200018
• Safe and rapid development of capillary electrophoresis for ultratrace uranyl ions in radioactive samples by way of fluorescent probe selection for actinide ions from a chemical library　　　　　　　　　　　　　　　
  Tomoko Haraga; Kazuki Ouchi; Yoshiyuki Sato; Hitoshi Hoshino; Rei Tanaka; Takashi Fujihara; Hideki Kurokawa; Masami Shibukawa; Ken-ichiro Ishimori; Yutaka Kameo; Shingo Saito
  Analytica Chimica Acta, 巻：1032, 開始ページ：188, 終了ページ：196, 2018年, ［査読有り］, ［責任著者］
  After the serious nuclear accident at the Fukushima Daiichi Nuclear Power Plant caused by the Great East Japan Earthquake in 2011, the development of feasible, safe, and highly sensitive analytical methods (in terms of low levels of radiation exposure and radioactive waste generation) for radioactive samples, especially actinide (An) ions, represents an important challenge. Here we propose a methodology for selecting appropriate emissive probes for An ions with very low consumption and emission of radioactivity by capillary electrophoresis–laser-induced fluorescence detection (CE-LIF), using a small chemical library of probes with eight different chelating moieties. It was found that the emissive probe L1, which possesses the tetradentate chelating moiety 1,10-phenanthroline-2,9-dicarboxylic acid (PDA), was suitable for detecting uranyl ions. The detection limit for the uranyl–L1 complex using CE-LIF combined with dynamic ternary complexation and on-capillary concentration techniques was determined to be 2.9 × 10−12 M (0.7 ppt). No interference from the large excess of matrix metal ions was observed. This method was successfully applied to real radioactive liquid samples collected from nuclear facilities, including the Fukushima Daiichi Nuclear Power Plant. This strategy not only permitted the development of a safe and rapid analytical method but also provided insight into the coordination chemistry of An ion complexes. Specifically, the PDA structure provided substantial kinetic inertness to its uranyl complex
  the formation of a ternary complex between uranyl–L1 and carbonate was revealed
  and unusual interactions were observed between the π-electron systems of uranyl and the phenanthroline ring, which stabilized the uranyl–PDA interaction.
  Elsevier B.V., 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.aca.2018.05.077
  DOI ID：10.1016/j.aca.2018.05.077, ISSN：1873-4324, SCOPUS ID：85048477881, Web of Science ID：WOS:000443002100021
• Synergistic effect of temperature and background counterions on ion-exchange equilibria　　　　　　　　　　　　　　　
  Shibukawa Masami; Yanagisawa Masaru; Morinaga Ryota; Shimasaki Tomomi; Saito Shingo; Wang Shao-ting; Feng Yu-qi
  RSC ADVANCES, 巻：8, 号：47, 開始ページ：26849, 終了ページ：26856, 2018年, ［査読有り］
  DOI：https://doi.org/10.1039/c8ra03309a
  DOI ID：10.1039/c8ra03309a, ISSN：2046-2069, Web of Science ID：WOS:000445745800046
• A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis　　　　　　　　　　　　　　　
  Kazuki Hirose; Maho Tsuchida; Hinako Asakura; Koji Wakui; Keitaro Yoshimoto; Keisuke Iida; Makoto Sato; Masami Shibukawa; Masami Suganuma; Shingo Saito
  ANALYST, 巻：142, 号：21, 開始ページ：4030, 終了ページ：4038, 2017年11月, ［査読有り］, ［責任著者］
  A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology called polymer-enhanced capillary transient isotachophoresis (PectI). The PectI separation yielded a single peak for the human lung cancer cell line (PC-9) complexed with DNA aptamer candidates, which was effectively separated from a free randomized DNA library peak, ensuring no contamination from free DNA in the PC-9-DNA aptamer complex fraction. The DNA aptamer candidates obtained after a single-round selection employing counter selection with HL-60 were proven to bind selectively and form kinetically stable complexes with PC-9 cells. Interestingly, most aptamer candidates showed high binding ability (K-d = 70-350 nM) with different extents of binding on the cell surface. These facts proved that a single-round selection for mammalian cells by PectI is feasible to obtain various types of aptamer candidates, which have high-affinity even for non-overexpressed but unique targets on the cell surface in addition to overexpressed targets.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c7an00909g
  DOI ID：10.1039/c7an00909g, ISSN：0003-2654, eISSN：1364-5528, Web of Science ID：WOS:000413562100003
• Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract　　　　　　　　　　　　　　　
  Takeharu Wada; I. Wayan Sumardika; Shingo Saito; I. Made Winarsa Ruma; Eisaku Kondo; Masami Shibukawa; Masakiyo Sakaguchi
  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 巻：1061, 開始ページ：209, 終了ページ：219, 2017年09月, ［査読有り］
  In accordance with our previous study that was carried out to identify novel anti-tumor ingredients, chromatographic separation in combination with an anti-tumor activity assay was used for analysis of Cordyceps militaris extract in this study. Various modes of chromatography including reversed-phase, cation-exchange and anion exchange were used to separate components of Cordyceps militaris, which showed various chemical properties. Anti-tumor activity of each fraction was assessed by a Hoechst staining-based apoptosis assay using malignant melanoma MeWo cells. By these repeated approaches through chromatographic segregation and cell biological assay, we finally succeeded in identifying the target substance from a certain fraction that included neutral hydrophilic components using a pre-column and post-column chlorine adduct ionization LC APCI MS method. The target substance was a mono-carbohydrate, xylitol, that induced apoptotic cell death in MeWo cells but not in normal human OUMS-24 fibroblasts. This is the first study showing that Cordyceps militaris extract contains a large amount of xylitol. Thus, our results will contribute greatly to uncovering the mysterious multifunctional herbal drug Cordyceps militaris as an anti-tumor agent.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.jchromb.2017.07.022
  DOI ID：10.1016/j.jchromb.2017.07.022, ISSN：1570-0232, eISSN：1873-376X, Web of Science ID：WOS:000411542000029
• Mechanism of ion stacking in aqueous partition chromatographic processes　　　　　　　　　　　　　　　
  Fouzia Akter; Yawara Ogiyama; Shingo Saito; Masami Shibukawa
  JOURNAL OF SEPARATION SCIENCE, 巻：40, 号：16, 開始ページ：3205, 終了ページ：3213, 2017年08月, ［査読有り］
  It has been reported that ion enrichment phenomena are observed in liquid chromatographic processes with an aqueous mobile phase on the columns packed with nonionic materials. However, the mechanism of the ion enrichment is not at all well understood. In this study, we investigated the retention and enrichment behaviors of simple inorganic anions on a C18-bonded silica column and a cross-linked hydroxylated methacrylic polymer gel column with pure aqueous mobile phases containing various electrolytes. We show that the stacking of ionic solutes can successfully be accounted for by the ion partition model, and it takes place due to the effect of the background coion in the eluent and/or sample solution on the distribution of the ions between the bulk water and the water incorporated in the packing material, which acts as the stationary phase. Using the ion exclusion effect of fixed anionic charges on a packing material as well as the ion stacking by partition, we developed a simple and versatile method for effective enrichment of anionic solutes in aqueous solutions. The enrichment factor and the elution time of the stacked ion zone can be predicted by the ion partition model.
  WILEY-V C H VERLAG GMBH, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/jssc.201700081
  DOI ID：10.1002/jssc.201700081, ISSN：1615-9306, eISSN：1615-9314, Web of Science ID：WOS:000409434100003
• キャピラリー電気泳動法を用いた放射性試料に対する簡易迅速分析法の開発　　　　　　　　　　　　　　　
  原賀 智子; 佐藤 義行; 亀尾 裕; 齋藤 伸吾
  デコミッショニング技報, 号：55, 開始ページ：22, 終了ページ：27, 2017年03月, ［査読有り］, ［最終著者］
  原子力バックエンド推進センター, 日本語
  ISSN：1343-3881, CiNii Articles ID：40021176640, CiNii Books ID：AA11695205
• Development and inter-laboratory study of a method for quantifying ochratoxin A in pet foods　　　　　　　　　　　　　　　
  T. Wada; H. Saito; K. Aoyama; S. Saito; M. Shibukawa
  WORLD MYCOTOXIN JOURNAL, 巻：10, 号：1, 開始ページ：53, 終了ページ：61, 2017年, ［査読有り］
  An analytical method for quantifying ochratoxin A (OTA) in pet foods using high-performance liquid chromatography was developed, and an inter-laboratory study was conducted. OTA was extracted from samples with aqueous acetonitrile. The extract was purified by an immunoaffinity column, OCHRAKING, and analysed by high performance liquid chromatography with fluorescence detection. The limits of quantification by this method were 2 mu g/kg for dry and semidry pet food and 1 mu g/kg for wet type pet food. The calibration curve showed linearity in the range of 0.550 ng/ml (equivalent to 1-100 mu g/kg for wet type pet food). The mean recoveries of OTA spiked at 1-5 mu g/kg were in the range of 83.0-106% and relative standard deviations of the in-house method validation were 2.6-6.8%. The mean recoveries, repeatability, reproducibility and the Horwitz ratios for OTA from the inter-laboratory validation study were 75.6-83.1%, 3.5-6.1%, 5.0-15.0% and 0.23-0.68, respectively.
  WAGENINGEN ACADEMIC PUBLISHERS, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.3920/WMJ2015.1953
  DOI ID：10.3920/WMJ2015.1953, ISSN：1875-0710, eISSN：1875-0796, Web of Science ID：WOS:000396027500005
• Superheated Water Ion-exchange Chromatography　　　　　　　　　　　　　　　
  Masami Shibukawa; Ryota Morinaga; Shingo Saito
  BUNSEKI KAGAKU, 巻：65, 号：11, 開始ページ：615, 終了ページ：623, 2016年11月, ［査読有り］
  Thermal effect on the separation selectivity of cation-exchange and anion-exchange processes was studied on a strongly acidic cation-exchange resin and a strongly basic anion-exchange resin using superheated water chromatography. The selectivity coefficient for a pair of identically charged inorganic ions, such as alkali metal ions and halide ions, approaches unity as the temperature increases, provided that the ions have the same effective size, such that the retention of an analyte ion decreases with an increase in temperature when the analyte ion has stronger affinity for the ion-exchanger than that of the eluent counter-ion, whereas it increases when it has weaker affinity. XAFS measurements on aqueous solutions containing the inorganic ions in the temperature range from ambient temperature to 175 degrees C at 3 MPa indicate that the number of coordinated first shell water molecules decreases with an increase in temperature. At elevated temperatures, electrostatic charge screening no longer effectively occurs, which may lead to smaller ion-exchange separation selectivity for different ions having an identical charge. Consequently, the electrostatic interaction or association of the ions with the fixed ion in the resin phase becomes predominant factors, resulting in a different separation selectivity from that obtained at ambient temperature.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.65.615
  DOI ID：10.2116/bunsekikagaku.65.615, ISSN：0525-1931, Web of Science ID：WOS:000389009900001
• Comparative analysis of distributions of radioactive cesium and potassium and stable cesium, potassium, and strontium in brown rice grains contaminated with radioactive materials released by the Fukushima Daiichi Nuclear Power Plant accident　　　　　　　　　　　　　　　
  M. Kato; Y. Okada; S. Hirai; Y. Minai; S. Saito; M. Shibukawa
  JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 巻：310, 号：1, 開始ページ：247, 終了ページ：252, 2016年10月, ［査読有り］
  The distributions of Cs-137, Cs-134, and K-40 as well as stable Cs, K, and Sr in bran, germ, and endosperm of brown rice contaminated by fallout from the Fukushima Daiichi Nuclear Power Plant were studied. It was found that Cs-137, Cs-134, and K-40 were accumulated in germ at the highest concentration and the concentration ratios of Cs and K in bran, germ, and endosperm were approximately identical to one another, while the highest concentration of Sr was detected for bran. Possible chemical forms of the metal elements in rice grains were discussed based on their extraction behavior with water and MgCl2 aqueous solution.
  SPRINGER, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s10967-016-4824-3
  DOI ID：10.1007/s10967-016-4824-3, ISSN：0236-5731, eISSN：1588-2780, Web of Science ID：WOS:000383579900025
• Selective Spectrophotometric Determination of Trace Amounts of Cadmium in Soil and Sediment Samples Using a Green Aqueous Biphasic Extraction　　　　　　　　　　　　　　　
  Masami Shibukawa; Daiki Nobushima; Shoko Sakuma; Mari Sasaki; Keisuke Nakamura; Yuma Matsutani; Kazunori Saitoh; Shingo Saito
  ANALYTICAL SCIENCES, 巻：32, 号：10, 開始ページ：1095, 終了ページ：1100, 2016年10月, ［査読有り］
  A green extraction spectrophotometric method was presented for the determination of trace amounts of cadmium in soil and sediment samples. This method is based on the selective extraction of cadmium as its iodide complex by aqueous biphasic extraction composed of polyethylene glycol (PEG) and sodium sulfate, and a subsequent sensitive determination by spectrophotometry using 2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol. This extraction method is simple and environmentally benign, since the organic solvents used for the traditional liquid-liquid extractions are replaced by the non-toxic polymer and inorganic salt. Cadmium can be selectively extracted from soil and sediment samples into the PEG-rich upper phase by an aqueous biphasic system containing potassium iodide and sulfuric acid. The proposed method was applied to the determination of cadmium in certified reference materials for soil and river sediment. The obtained results were in good agreement with the certified values.
  JAPAN SOC ANALYTICAL CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.32.1095
  DOI ID：10.2116/analsci.32.1095, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000386423300011
• Erratum: Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces (Anal. Chem. (2015) 87(1180-1187)DOI: 10.1021/ac503802q)
  Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
  Analytical Chemistry, 巻：88, 開始ページ：1065, 2016年01月
  DOI：https://doi.org/10.1021/acs.analchem.5b04447
  DOI ID：10.1021/acs.analchem.5b04447, ISSN：0003-2700, SCOPUS ID：84953439586
• Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis　　　　　　　　　　　　　　　
  Shingo Saito; Kazuki Hirose; Maho Tsuchida; Koji Wakui; Keitaro Yoshimoto; Yoshitaka Nishiyama; Masami Shibukawa
  CHEMICAL COMMUNICATIONS, 巻：52, 号：3, 開始ページ：461, 終了ページ：464, 2016年, ［査読有り］, ［筆頭著者, 責任著者］
  We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity (kinetically inert) DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles (free cells and cells bound with aptamers) and molecules (unbound or dissociated DNA) in free solution.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c5cc07268a
  DOI ID：10.1039/c5cc07268a, ISSN：1359-7345, eISSN：1364-548X, Web of Science ID：WOS:000367259200005
• Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces (vol 87, pg 1180, 2015)　　　　　　　　　　　　　　　
  Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
  ANALYTICAL CHEMISTRY, 巻：88, 号：1, 開始ページ：1065, 終了ページ：1065, 2016年01月, ［査読有り］
  AMER CHEMICAL SOC, 英語
  DOI：https://doi.org/10.1021/acs.analchem.5b04447
  DOI ID：10.1021/acs.analchem.5b04447, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000367866100097
• Affinity Capillary Electrophoresis for Selective Control of Electrophoretic Mobility of Sialic Acid Using Lanthanide-Hexadentate Macrocyclic Polyazacarboxylate Complexes　　　　　　　　　　　　　　　
  Daiki Goto; Kazuki Ouchi; Masami Shibukawa; Shingo Saito
  ANALYTICAL SCIENCES, 巻：31, 号：11, 開始ページ：1143, 終了ページ：1149, 2015年11月, ［査読有り］, ［責任著者］
  It is difficult to control the electrophoretic mobility in order to obtain high resolution among saccharides in complex samples. We report herein on a new affinity capillary electrophoresis (ACE) method for an anionic monosaccharide, N-acetylneuraminic acid (Neu5Ac), which is important in terms of pathological diagnosis, using lanthanide-hexadentate macrocyclic polyazacarboxylate complexes (Ln-NOTA) as affinity reagents. It was shown that Ln-NOTA complexes increased the anionic mobility of Neu5Ac by approximately 40% through selective complexation with Neu5Ac. The extent of change in the mobility strongly depended on the type of central metal ion of Ln-NOTA. The stability constant (K) of Lu-NOTA with Neu5Ac was determined by ACE to be log K-b = 3.62 +/- 0.04, which is the highest value among artificial receptors for Neu5Ac reported so far. Using this ACE, the Neu5Ac content in a glycoprotein sample, alpha 1-acid glycoprotein (AGP), was determined after acid hydrolysis. Complete separation between Neu5Ac and hydrolysis products was successful by controlling the mobility to determine the concentration of Neu5Ac.
  JAPAN SOC ANALYTICAL CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.31.1143
  DOI ID：10.2116/analsci.31.1143, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000365375700007
• Determination of the cis-trans lsomerization Barriers of L-Alanyl-L-proline in Aqueous Solutions and at Water/Hydrophobic Interfaces by On-Line Temperature-Jump Relaxation HPLC and Dynamic On-Column Reaction HPLC　　　　　　　　　　　　　　　
  Masami Shibukawa; Ayaka Miyake; Sayaka Eda; Shingo Saito
  ANALYTICAL CHEMISTRY, 巻：87, 号：18, 開始ページ：9280, 終了ページ：9287, 2015年09月, ［査読有り］
  Praline cis-trans isomerization is known to play a key role in the rate-determining steps of protein folding. It is thus very important to understand the influence of environments, not only bulk solutions but also microenvironments such as interfaces, on the isomerization reaction of proline peptides. Here we present two HPLC methods for measurements of kinetic and equilibrium parameters for the isomerization reactions in bulk solutions and at liquid/solid interfaces. On-line temperature-jump relaxation HPLC (T-jump HPLC) allows the determination of forward and reverse rate constants of the isomerization in a bulk solution by monitoring the whole time course of conversion of pure isomers from both sides of the reaction, in contrast to other HPLC and capillary zone electrophoresis as well as spectrometric and calorimetric methods, which use a mixture of the isomers. We can then determine cis-trans isomerization barriers of the peptide at liquid/solid interfaces from the kinetic data obtained by dynamic on-column reaction HPLC and T-jump HPLC. We observed that the interconversion around the peptide bond for L-alanyl-L-proline (Ala-Pro) in water is accelerated at the surfaces of an alkyl-bonded silica and a poly(styrene divinylbenzene) copolymer resin, and this is caused by a remarkable decrease in the enthalpy of activation. The molecular structures of the cis and trans forms of Ala-Pro estimated by quantum mechanics calculation reveal that an equilibrium shift toward the cis form as well as the rapid isomerization of Ala-Pro at the water/hydrophobic interfaces can be attributed to the lower polarity of the interfacial water at the surfaces of the hydrophobic materials compared to that of bulk water.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/acs.analchem.5b02488
  DOI ID：10.1021/acs.analchem.5b02488, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000361416000025
• c-Type Cytochrome Assembly Is a Key Target of Copper Toxicity within the Bacterial Periplasm　　　　　　　　　　　　　　　
  Anne Durand; Asma Azzouzi; Marie-Line Bourbon; Anne-Soisig Steunou; Sylviane Liotenberg; Akinori Maeshima; Chantal Astier; Manuela Argentini; Shingo Saito; Soufian Ouchane
  MBIO, 巻：6, 号：5, 2015年09月, ［査読有り］
  In the absence of a tight control of copper entrance into cells, bacteria have evolved different systems to control copper concentration within the cytoplasm and the periplasm. Central to these systems, the Cu+ ATPase CopA plays a major role in copper tolerance and translocates copper from the cytoplasm to the periplasm. The fate of copper in the periplasm varies among species. Copper can be sequestered, oxidized, or released outside the cells. Here we describe the identification of CopI, a periplasmic protein present in many proteo bacteria, and show its requirement for copper tolerance in Rubrivivax gelatinosus. The Delta copI mutant is more susceptible to copper than the Cu+ ATPase copA mutant. CopI is induced by copper, localized in the periplasm and could bind copper. Interestingly, copper affects cytochrome c membrane complexes (cbb(3) oxidase and photosystem) in both Delta copI and copA-null mutants, but the causes are different. In the copA mutant, heme and chlorophyll synthesis are affected, whereas in Delta copI mutant, the decrease is a consequence of impaired cytochrome c assembly. This impact on c-type cytochromes would contribute also to the copper toxicity in the periplasm of the wild-type cells when they are exposed to high copper concentrations.
  IMPORTANCE Copper is an essential cation required as a cofactor in enzymes involved in vital processes such as respiration, photosynthesis, free radical scavenging, and pathogenesis. However, copper is highly toxic and has been implicated in disorders in all organisms, including humans, because it can catalyze the production of toxic reactive oxygen species and targets various biosynthesis pathways. Identifying copper targets, provides insights into copper toxicity and homeostatic mechanisms for copper tolerance. In this work, we describe for the first time a direct effect of excess copper on cytochrome c assembly. We show that excess copper specifically affects periplasmic and membrane cytochromes c, thus suggesting that the copper toxicity targets c-type cytochrome biogenesis.
  AMER SOC MICROBIOLOGY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1128/mBio.01007-15
  DOI ID：10.1128/mBio.01007-15, ISSN：2150-7511, Web of Science ID：WOS:000364523100013
• Molecular Design of Boronic Acid-Functionalized Squarylium Cyanine Dyes for Multiple Discriminant Analysis of Sialic Acid in Biological Samples: Selectivity toward Monosaccharides Controlled by Different Alkyl Side Chain Lengths　　　　　　　　　　　　　　　
  Kazuki Ouchi; Christa L. Colyer; Mahmoud Sebaiy; Jin Zhou; Takeshi Maeda; Hiroyuki Nakazumi; Masami Shibukawa; Shingo Saito
  ANALYTICAL CHEMISTRY, 巻：87, 号：3, 開始ページ：1933, 終了ページ：1940, 2015年02月, ［査読有り］, ［責任著者］
  We designed a new series of boronic acid-functionalized squarylium cyanine dyes (SQ-BA) with different lengths of alkyl chain residues, suitable for multiple discriminant analysis (MDA) of sialic acid (Neu5Ac) in biological samples. The SQ-BA dyes form aggregates based on hydrophobic interactions, which result in quenched fluorescence in aqueous solutions. When the boronic acid binds with saccharides, the fluorescence intensity increases as a result of dissociation to the emissive monomeric complex. We inferred that different dye aggregate structures (H-aggregates and J-aggregates) were induced depending on the alkyl chain length, so that monosaccharides would be recognized in different ways (especially, multipoint interaction with J-aggregates). A distinctive emission enhancement of SQ-BA dyes with shorter-alkyl-chains in the presence of Neu5Ac was observed (2.4-fold fluorescence enhancement; with formation constant 10(1.7) M-1), with no such enhancement for SQ-BA dyes with longer-alkyl-chain. In addition, various enhancement factors for other monosaccharides were observed depending on the alkyl chain length. Detailed thermodynamic and NMR studies of the SQ-BA complexes revealed the unique recognition mechanism: the dye aggregate with a shorter-alkyl-chain causes the slipped parallel structure and forms a stable 2:1 complex with Neu5Ac, as distinct from longer-alkyl-chain dyes, which form a 1:1 monomeric complex. MDA using the four SQ-BA dyes was performed for human urine samples, resulting in the successful discrimination between normal and abnormal Neu5Ac levels characteristic of disease. Thus, we successfully controlled various responses to similar monosaccharides with a novel approach that chemically modified not the boronic acid moiety itself but the length of the alkyl chain residue attached to the dye in order to generate specificity.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac504201b
  DOI ID：10.1021/ac504201b, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000349059000071
• Combining capillary electrophoresis and next-generation sequencing for aptamer selection　　　　　　　　　　　　　　　
  Kathryn R. Riley; Jason Gagliano; Jiajie Xiao; Kara Libby; Shingo Saito; Guo Yu; Roger Cubicciotti; Jed Macosko; Christa L. Colyer; Martin Guthold; Keith Bonin
  ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 巻：407, 号：6, 開始ページ：1527, 終了ページ：1532, 2015年02月, ［査読有り］
  Next-generation sequencing (NGS) machines can sequence millions of DNA strands in a single run, such as oligonucleotide (oligo) libraries comprising millions to trillions of discrete oligo sequences. Capillary electrophoresis is an attractive technique to select tight binding oligos or "aptamers" because it requires minimal sample volumes (e.g., 100 nL) and offers a solution-phase selection environment through which enrichment of target-binding oligos can be determined quantitatively. We describe here experiments using capillary transient isotachophoresis (ctITP)-based nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) as a method for selecting aptamers from a randomized library containing a known (29mer) thrombin-binding aptamer. Our capillary electrophoresis (CE)-selected samples were sequenced by the Ion Torrent Personal Genome Machine (PGM) and analyzed for selection efficiency. We show that a single round of CE selection can enrich a randomer synthetic DNA oligo mixture for thrombin-binding activity from 0.4 % aptamer content before selection to > 15 % aptamer content.
  SPRINGER HEIDELBERG, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s00216-014-8427-y
  DOI ID：10.1007/s00216-014-8427-y, ISSN：1618-2642, eISSN：1618-2650, Web of Science ID：WOS:000349337100001
• Surface-Bubble-Modulated Liquid Chromatography: A New Approach for Manipulation of Chromatographic Retention and Investigation of Solute Distribution at Water/Hydrophobic Interfaces　　　　　　　　　　　　　　　
  Keisuke Nakamura; Hiroki Nakamura; Shingo Saito; Masami Shibukawa
  ANALYTICAL CHEMISTRY, 巻：87, 号：2, 開始ページ：1180, 終了ページ：1187, 2015年01月, ［査読有り］
  In this paper, we present a new chromatographic method termed surface-bubble-modulated liquid chromatography (SBMLC), that has a hybrid separation medium incorporated with surface nanobubbles. Nanobubbles or nanoscale gas phases can be fixed at the interface between water and a hydrophobic material by delivering water into a dry column packed with a nanoporous material. The incorporation of a gas phase at the hydrophobic surface leads to the formation of the hybrid separation system consisting of the gas phase, hydrophobic moieties, and the water/hydrophobic interface or the interfacial water. One can change the volume of the gas phase by pressure applied to the column, which in turn alters the area of water/hydrophobic interface or the volume of the interfacial water, while the amount of the hydrophobic moiety remains constant. Therefore, this strategy provides a novel technique not only for manipulating the separation selectivity by pressure but also for elucidating the mechanism of accumulation or retention of solute compounds in aqueous solutions by a hydrophobic material. We evaluate the contributions of the interfacial water at the surface of an octadecyl bonded silica and the bonded layer itself to the retention of various solute compounds in aqueous solutions on the column packed with the material by SBMLC. The results show that the interfacial water formed at the hydrophobic surface has a key role in retention even though its volume is rather small. The manipulation of the separation selectivity of SBMLC for some organic compounds by pressure is demonstrated.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac503802q
  DOI ID：10.1021/ac503802q, ISSN：0003-2700, eISSN：1520-6882, Web of Science ID：WOS:000348332300049
• Facilitating aptamer selection and collection by capillary transient isotachophoresis with laser-induced fluorescence detection　　　　　　　　　　　　　　　
  Kathryn R. Riley; Shingo Saito; Jason Gagliano; Christa L. Colyer
  JOURNAL OF CHROMATOGRAPHY A, 巻：1368, 開始ページ：183, 終了ページ：189, 2014年11月, ［査読有り］
  An efficient separation method that utilizes capillary transient isotachophoresis (ctITP) was developed for the preselection of binding ligands. With the ultimate goal of providing enriched fractions from vast libraries for drug discovery, the preselection process described herein entails three distinct elements, which have been validated using a model thrombin protein (target) and thrombin aptamer (ligand) system. First, a high fidelity, on-column labeling scheme employing the noncovalent, fluorescent reagent SYBR Gold was demonstrated for single-stranded DNA with an 11-fold greater sensitivity than pre-column labeling procedures. Second, this on-column labeling was incorporated into a new ctITP method with laser-induced fluorescence (LIF) detection, which provided greatly enhanced resolution of protein-aptamer complex and free aptamer (in comparison to traditional capillary zone electrophoresis (CZE) methods). Third, this enhanced resolution permitted the subsequent accumulation of bound aptamer fractions via an automated collection method, with the establishment of quantitative measures of DNA accumulation. Preselected aptamer or ligand samples such as these can serve as inputs for subsequent lab-on-bead or next-generation-sequencing technologies, enabling accelerated drug discovery. (C) 2014 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2014.09.062
  DOI ID：10.1016/j.chroma.2014.09.062, ISSN：0021-9673, eISSN：1873-3778, Web of Science ID：WOS:000344437500020
• Application of Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Trace Neodymium in Spent Nuclear Fuel Using Complexation with an Emissive Macrocyclic Polyaminocarboxylate Probe　　　　　　　　　　　　　　　
  Tomoko Haraga; Shingo Saito; Yoshiyuki Sato; Shiho Asai; Yukiko Hanzawa; Hitoshi Hoshino; Masami Shibukawa; Ken-ichiro Ishimori; Kuniaki Takahashi
  ANALYTICAL SCIENCES, 巻：30, 号：7, 開始ページ：773, 終了ページ：776, 2014年07月, ［査読有り］, ［責任著者］
  A simple and rapid method with low radiation exposure risk was developed for the determination of neodymium in spent nuclear fuel by capillary electrophoresis with laser-induced fluorescence detection using a fluorescent probe having a macrocyclic hexadentate polyaminocarboxylate structure. The concentration of Nd(III) in a spent nuclear fuel sample was determined with no interference from various matrix elements, including lanthanides and uranium (at a 200-fold excess), with 92 +/- 3% recovery. This is due to high resolution based on establishing a ternary complex equilibrium during migration in which the hydroxyl ion plays an auxiliary role (log KLn-L-OH = 3.9 - 5.3).
  JAPAN SOC ANALYTICAL CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.30.773
  DOI ID：10.2116/analsci.30.773, ISSN：0910-6340, eISSN：1348-2246, Web of Science ID：WOS:000339461400011
• タンパク質結合型金属イオンのポリアクリルアミドゲル電気泳動によるマッピング　　　　　　　　　　　　　　　
  齋藤 伸吾
  生物物理化学, 巻：58, 号：2, 開始ページ：24, 終了ページ：26, 2014年
  Japanese Electrophoresis Society, 日本語
  DOI：https://doi.org/10.2198/sbk.58.24
  DOI ID：10.2198/sbk.58.24, ISSN：0031-9082, CiNii Articles ID：130004704677, CiNii Books ID：AN00129729
• Determination of Free Lime in Steelmaking Slags by Use of Ethylene Glycol Extraction/ICP-AES and Thermogravimetry　　　　　　　　　　　　　　　
  Misa Kato; Tatsuya Hari; Shingo Saito; Masami Shibukawa
  TETSU TO HAGANE-JOURNAL OF THE IRON AND STEEL INSTITUTE OF JAPAN, 巻：100, 号：3, 開始ページ：340, 終了ページ：345, 2014年, ［査読有り］
  Steelmaking slags are mainly used as aggregates for road and civil constructions and slag products successfully replace natural sand and macadam. However, the slags may contain appreciable amounts of free lime (CaO) that can cause expansive self-destruction by reactions with water and carbon dioxide in the air. It is thus very important in evaluating the quality of the slags as raw materials to develop an accurate and precise method for determination of free lime in the slags. In the present work we developed a method for determination of free lime in the slags that consists of extraction with ethylene glycol followed by inductively coupled plasma atomic emission spectrometry (ICP-AES) and thermogravimetry (TG). The ethylene glycol extraction method (EG method) has been used as a conventional method for analysis of free lime in steelmaking slags. It has been known, however, that the analytical results obtained by the EG method are not reproducible and ethylene glycol dissolves not only CaO but also Ca(OH)(2). We re-examined the EG method and optimized the conditions for complete extraction of both CaO and Ca(OH)(2) in the slags. We also found that TG enables one to determine Ca(OH)(2) in steelmaking slags. The content of CaO in a slag sample is calculated from the amount of calcium determined by the improved EG method and that of Ca(OH)(2) obtained by TG.
  IRON STEEL INST JAPAN KEIDANREN KAIKAN, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2355/tetsutohagane.100.340
  DOI ID：10.2355/tetsutohagane.100.340, ISSN：0021-1575, eISSN：1883-2954, Web of Science ID：WOS:000332682600002
• Separation of metalloproteins using a novel metal ion contaminant sweeping technique and detection of protein-bound copper by a metal ion probe in polyacrylamide gel electrophoresis: Distribution of copper in human serum　　　　　　　　　　　　　　　
  Shingo Saito; Mitsuyoshi Kawashima; Hiroki Ohshima; Kazuki Enomoto; Makoto Sato; Hajime Yoshimura; Keitaro Yoshimoto; Mizuo Maeda; Masami Shibukawa
  Analyst, 巻：138, 号：20, 開始ページ：6097, 終了ページ：6105, 2013年10月, ［査読有り］, ［筆頭著者, 責任著者］
  A polyacrylamide gel electrophoresis (PAGE)-based method has been developed, consisting of two types of gel electrophoresis, to obtain an accurate distribution of protein-bound metal ions in biological samples. First, proteins are separated by PAGE without the uptake of contaminant metal ions in the separation field and dissociation of metal ions from the proteins. This is followed by another PAGE for the separation and detection of protein-bound metal ions in small volume samples with high sensitivity in the ppt range using a fluorescent metal probe. The former is a new technique using blue-native (BN) PAGE to electrophoretically sweep all metal contaminants by employing two kinds of chelating agents. These agents form complexes with contaminants in the gel and the separation buffer solution, which migrate towards opposite pole directions, thus lowering the contaminants to below the ppt level during separation. This is termed "Metal Ion Contaminant Sweeping BN-PAGE (MICS-BN-PAGE)". After the separation of proteins under these first metal-free conditions, the metal ions in the gel fractions are eluted, followed by derivatization of copper ions into the metal probe complexes to be separated and determined by fluorescence detection in the second PAGE. In this PAGE-based method, the copper ions bound to ceruloplasmin and superoxide dismutase were quantitatively determined, in addition to the exchangeable albumin-bound copper ions. This system successfully provided distribution maps of protein-copper in human serum. The precise distribution of copper in human serum was investigated, and found to be different from that which is widely accepted. © 2013 The Royal Society of Chemistry.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c3an01107k
  DOI ID：10.1039/c3an01107k, ISSN：0003-2654, PubMed ID：23964357, SCOPUS ID：84884222364, Web of Science ID：WOS:000324545000031
• New molecular motif for recognizing sialic acid using emissive lanthanide-macrocyclic polyazacarboxylate complexes: Deprotonation of a coordinated water molecule controls specific binding　　　　　　　　　　　　　　　
  Kazuki Ouchi; Shingo Saito; Masami Shibukawa
  Inorganic Chemistry, 巻：52, 号：11, 開始ページ：6239, 終了ページ：6241, 2013年06月, ［査読有り］, ［責任著者］
  A new molecular motif -lanthanide-macrocyclic polyazacarboxylate hexadentate complexes, Ln3+-ABNOTA -was found to specifically bind to sialic acid with strong emission enhancement and high affinity. The selectivity toward sialic acid over other monosaccharides was one of the highest among artificial receptors. Also, the novel binding mechanism was investigated in detail
  binding selectivity is controlled by interactions between sialic acid and both the central metal and a hydroxyl group produced by deprotonation of a coordinated water molecule in the Ln3+ complex. © 2013 American Chemical Society.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ic400725a
  DOI ID：10.1021/ic400725a, ISSN：0020-1669, PubMed ID：23697912, SCOPUS ID：84878636933, Web of Science ID：WOS:000320015700003
• An Application of Polymer-Enhanced Capillary Transient Isotachophoresis with an Emissive Boronic Acid Functionalized Squarylium Dye as an On-Capillary Labeling Agent for Gram-positive Bacteria　　　　　　　　　　　　　　　
  Shingo Saito; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
  ANALYTICAL SCIENCES, 巻：29, 号：1, 開始ページ：157, 終了ページ：159, 2013年01月, ［査読有り］, ［筆頭著者］
  In this paper, the characterization and application of the "PeaI" (polymer-enhanced capillary transient isotachophoresis) technique for the separation and detection of same genus, gram-positive bacteria, Bacillus globigii (Bg) and Bacillus subtilis, is demonstrated by employing a boronic acid-functionalized squarylium dye (SQ-BA) as an on-capillary labeling agent, including the quantitative performance and applicability to crude samples. The effect of borate in the separation buffer was also investigated, which revealed that borate strongly affects the separation behavior of bacteria.
  JAPAN SOC ANALYTICAL CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.29.157
  DOI ID：10.2116/analsci.29.157, ISSN：0910-6340, Web of Science ID：WOS:000314332200029
• Multistep pH-Peak-Focusing Countercurrent Chromatography with a Polyethylene Glycol-Na2SO4 Aqueous Two Phase System for Separation and Enrichment of Rare Earth Elements　　　　　　　　　　　　　　　
  Kohei Shimizu; Hiroaki Kuribayashi; Haruna Watanabe; Tomomi Shimasaki; Kenzaburo Azuma; Yohei Horie; Kazunori Saitoh; Shingo Saito; Masami Shibukawa
  ANALYTICAL CHEMISTRY, 巻：85, 号：2, 開始ページ：978, 終了ページ：984, 2013年01月, ［査読有り］
  Multistep pH-peak-focusing countercurrent chromatography was developed for separation and enrichment of rare earth metal ions using a polyethylene glycol-Na2SO4 aqueous two phase system (ATPS) and pH stepwise gradient elution. Metal ions in a sample solution are chromatographically extracted in a basic stationary phase (polymer-rich phase of the ATPS) containing a complexation ligand such as acetylacetone at the top of the countercurrent chromatography (CCC) column. After the sample solution is introduced, the mobile phases of which the pH values have been adjusted with buffer reagents are delivered into the column by stepwise gradient elution in order of decreasing pH. Each metal ion is concentrated at a pH border formed between the zones of different pH in the CCC column through extraction with a complexing agent into the stationary phase at the front side of the border (basic region) and back extraction into the mobile phase at the back side of the border (acidic region), moving toward the outlet of the column with the pH border. Mutual separations of La(III), Ce(III), Nd(III), Yb(III), and Sc(III) were achieved by the present method using five step pH gradient elution, and each rare earth metal ion was effectively enriched at each of the five pH borders. The mechanism for formation of pH profile of the column effluent and the potential of this technique for preparative scale separation are also discussed.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac302546s
  DOI ID：10.1021/ac302546s, ISSN：0003-2700, Web of Science ID：WOS:000313668400047
• A Long-Wavelength Fluorescent Squarylium Cyanine Dye Possessing Boronic Acid for Sensing Monosaccharides and Glycoproteins with High Enhancement in Aqueous Solution　　　　　　　　　　　　　　　
  Shingo Saito; Tara L. Massie; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
  SENSORS, 巻：12, 号：5, 開始ページ：5420, 終了ページ：5431, 2012年05月, ［査読有り］, ［筆頭著者］
  Fluorescence sensing of saccharides and glycoproteins using a boronic acid functionalized squarylium cyanine dye ("SQ-BA") is characterized in terms of synthetic, fluorometric, thermodynamic and kinetic parameters. In our previous work, this newly synthesized dye was successfully applied to the separation and quantification of Gram-positive bacteria by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF); however, the fundamental properties of the dye and its saccharide complexes still required elucidation, as presented in this paper. The dye itself forms nonemissive, soluble aggregates in aqueous solution. With the addition of a monosaccharide, the dye aggregate dissociates to form an emissive monomer accompanied by the formation of a cyclic cis-diol ester with long-wavelength emission (lambda(ex) = 630 nm, lambda(em) = 660 nm). A very large fluorescence enhancement factor of 18x was observed for the sensing dye as a fructose complex at pH 10, yielding a limit of detection of 10 mu M fructose. The relative order of fluorescence enhancement of SQ-BA with other monosaccharides was found to be: fructose > ribose > arabinose approximate to galactose > xylose > mannose > rhamnose > fucose approximate to glucose; and apparent affinity constants of 10(2.80), 10(2.08) and 10(0.86) M-1 were determined for fructose, ribose and glucose, respectively. Formation of the emissive complexes occurred within minutes, proving the kinetics of the sugar-dye interactions to be suitable for on-column labeling methods in CE-LIF. Furthermore, the sensing dye was successfully applied to glycoproteins, mucin type I-S and type III, which were detected with high sensitivity in batch aqueous solution as a result of the sugar-selective boronic acid-diol esterification as well as hydrophobic interactions.
  MDPI AG, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.3390/s120505420
  DOI ID：10.3390/s120505420, ISSN：1424-8220, Web of Science ID：WOS:000304539200013
• Highly sensitive detection of neodymium ion in small amount of spent nuclear fuel samples using novel fluorescent macrocyclic hexadentate polyaminocarboxylate probe in capillary electrophoresis-laser-induced fluorescence detection　　　　　　　　　　　　　　　
  Shingo Saito; Yoshiyuki Sato; Tomoko Haraga; Yuta Nakano; Shiho Asai; Yutaka Kameo; Kuniaki Takahashi; Masami Shibukawa
  JOURNAL OF CHROMATOGRAPHY A, 巻：1232, 開始ページ：152, 終了ページ：157, 2012年04月, ［査読有り］, ［筆頭著者, 責任著者］
  A rapid and high-sensitive detection method for the total concentration of Nd ion (total Nd) in a small amount of a spent nuclear fuel sample is urgently required since the precise quantification of total Nd ion makes it possible for burnup (degree of fuel consumption) to be determined. In this work, a capillary electrophoresis-laser-induced fluorescent detection method (CE-LIF) is proposed for the analysis of total Nd in a spent fuel sample solution, with the use of a newly synthesized metal fluorescent probe which has a fluorescein and a macrocylic hexadentate chelating group, FTC-ABNOTA, for lanthanide (Ln) ions. Ln ions were derivatized to form a strongly fluorescent complex with the probe to suppress the quenching of the ligand-centered emission. The detection of Ln complexes in the CE-LIF indicated that the interaction between Ln ions and the FTC-ABNOTA was strong enough not to dissociate during migration. The mutual separation among the Ln-FTC-ABNOTA complexes in CE-LIF was achieved by pH control providing a dynamic ternary complexation (DTC) with hydroxide ions. Using the DTC separation mode, a high resolution of Nd from other Ln ions with high resolution of 1.3-1.9 and a theoretical plate number of 68,000, and a very low detection limit of 22 pM (3.2 ppt, 0.11 attomole amount basis) were successfully obtained. A simulated spent fuel sample containing various metal ions was examined in this method with a good quantification result of 102.1% recovery obtained even with a large excess of U. (C) 2011 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2011.11.032
  DOI ID：10.1016/j.chroma.2011.11.032, ISSN：0021-9673, Web of Science ID：WOS:000302423000021
• On-Column Labeling of Gram-Positive Bacteria with a Boronic Acid Functionalized Squarylium Cyanine Dye for Analysis by Polymer-Enhanced Capillary Transient Isotachophoresis　　　　　　　　　　　　　　　
  Shingo Saito; Tara L. Massie; Takeshi Maeda; Hiroyuki Nakazumi; Christa L. Colyer
  ANALYTICAL CHEMISTRY, 巻：84, 号：5, 開始ページ：2452, 終了ページ：2458, 2012年03月, ［査読有り］, ［筆頭著者］
  A new asymmetric, squarylium cyanine dye functionalized by boronic acid ("SQ-BA") was designed and synthesized for on-capillary labeling of gram-positive bacteria to provide for high sensitivity detection by way of a modified form of capillary electrophoresis with laser induced fluorescence detection (CE-LIF). The CE-based separation employed a polymer-enhanced buffer with capillary transient isotachophoresis in a new hybrid method dubbed "PectI." It was found that the addition of various monosaccharides to SQ-BA in a batch aqueous solution greatly enhanced the emission of the boronic acid functionalized dye by a factor of up to 18.3 at a long wavelength (lambda(ex) = 630 nm, lambda(em) = 660 nm) with a high affinity constant (K = similar to 10(2.80) M-1) superior to other sugar probes. Semiempirical quantum mechanics calculations suggest that the mechanism for this high enhancement may involve the dissociation of initially nonemissive dye associates (stabilized by an intramolecular hydrogen bond) upon complex formation with sugars. The fluorescence emission of SQ-BA was also significantly enhanced in the presence of a gram-positive bacterial spore, Bacillus globigii (Bg), which serves as a simulant of B. anthracis (or anthrax) and which possesses a peptidoglycan (sugar)-rich spore coat to provide ample sites for interaction with the dye. Several peaks were observed for a pure Bg sample even with polyethyleneoxide (PEO) present in the CE separation buffer, despite the polymer's previously demonstrated ability to focus microoorganisms to a single peak during migration. Likewise, several peaks were observed for a Bg sample when capillary transient isotachophoresis (ctITP) alone was employed. However, the new combination of these techniques as "PectI" dramatically and reproducibly focused the bacteria to a single peak with no staining procedure. Using PectI, the trace detection of Bg spores (corresponding to approximately three cells per injection) along with separation efficiency enough to separate Bg from another gram-positive bacteria, Saccharomyces cerevisiae (resolution, R-s = 6.09, and apparent plate number, N = 2.7-3.3 x 10(5)), were successfully achieved.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac2031145
  DOI ID：10.1021/ac2031145, ISSN：0003-2700, Web of Science ID：WOS:000301021400050
• 放射性廃棄物中のウランの分析のための高感度蛍光プローブを用いたキャピラリー電気泳動－レーザー励起蛍光検出法　　　　　　　　　　　　　　　
  原賀 智子; 佐藤 義行; 渋川 雅美; 亀尾 裕; 高橋 邦明; 齋藤 伸吾
  日本原子力学会 年会・大会予稿集, 巻：2012, 開始ページ：458, 終了ページ：458, 2012年
  放射性廃棄物の処分に向けて、廃棄物試料中のアクチニド（Th、U、Np、Pu、Am、Cm）を簡易かつ高感度に分析する方法を開発することを目的として、キャピラリー電気泳動－レーザー励起蛍光検出法（CE-LIF）の適用性について検討している。本検討では、ウランを分析対象として、ウラニルイオンと安定な錯体を形成する配位骨格を有する蛍光プローブを新規に合成し、CE-LIFによりウラニルイオンの分離検出を試みた。その結果、溶液試料において、従来のCE法の検出限界（数十ppb－ppmレベル）を大幅に改善した数十pptレベルの検出限界を達成できた。
  一般社団法人 日本原子力学会, 日本語
  DOI：https://doi.org/10.11561/aesj.2012f.0.458.0
  DOI ID：10.11561/aesj.2012f.0.458.0, CiNii Articles ID：130005051757
• XAFS Study of Thermal Effect on Hydration Structures of Inorganic Ions　　　　　　　　　　　　　　　
  Masami Shibukawa; Yawara Ogiyama; Tomomi Shimasaki; Yoshiki Kondo; Asako Inoue; Shingo Saito
  Photon Factory Activity Report, 2012年
• X-Ray absorption fine structure spectroscopy studies of thermal effects on ion-exchange equilibria　　　　　　　　　　　　　　　
  Masami Shibukawa; Makoto Harada; Tetsuo Okada; Yawara Ogiyama; Tomomi Shimasaki; Yoshiki Kondo; Asako Inoue; Shingo Saito
  RSC ADVANCES, 巻：2, 号：24, 開始ページ：8985, 終了ページ：8991, 2012年, ［査読有り］
  The effect of temperature on the first-shell coordination structures for Rb+, Sr2+, Br-, and I- in aqueous solution and those for Rb+ and Sr2+ in a sulfonated styrene-divinylbenzene copolymer cation-exchange resin have been evaluated by X-ray absorption fine structure (XAFS) spectroscopy in order to elucidate the origin of the effect of temperature on ion-exchange equilibria. XAFS measurements on aqueous solutions containing the inorganic ions in the temperature range from ambient temperature to 175 degrees C at 3 MPa indicate that the number of coordinated first shell water molecules decreases with an increase in temperature, while the first shell ion-oxygen (water) bond length remains almost unchanged (Sr2+ and I-) or slightly decreases (Rb+ and Br-). The spectral changes observed for Rb+ and Sr2+ in the hydrated cation-exchange resin were nearly the same as those for the ions in aqueous solution, though there is an indication for the contact ion pairs with the fixed sulfonate groups at high temperatures. These data represent the direct spectroscopic confirmation of structural change around inorganic ions in an ion-exchange resin as well as in aqueous solutions. The effect of temperature on separation selectivity in ion-exchange processes can be interpreted by the change in hydration of ions clarified in the present study.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c2ra21278a
  DOI ID：10.1039/c2ra21278a, ISSN：2046-2069, Web of Science ID：WOS:000308824600018
• Determination of Trace Amount of Cobalt in a Steel Sample by Two-dimensional On-line Redox Derivatization Liquid Chromatography　　　　　　　　　　　　　　　
  Masami Shibukawa; Akira Yamazaki; Shingo Saito; Koichi Saitoh
  ISIJ INTERNATIONAL, 巻：52, 号：9, 開始ページ：1622, 終了ページ：1626, 2012年, ［査読有り］
  A two-dimensional on-line redox derivatization HPLC system was developed where "heart-cutting" chromatography, in conjunction with on-line redox derivatizaion, was used to isolate specific analytes in complex matrix samples. Cobalt in a stainless steel sample was used as a model analyte to evaluate the performance of the HPLC system developed. We adopted a small column packed with porous graphitic carbon (PGC) treated with hydrogen peroxide as an oxidation derivatization unit and two C-18 silica columns which had adsorbed hexadecyltrimethylammonium ion as the first and second separation columns. After complexation of metal components with ethylenediaminetetraacetate (edta), the sample was directly submitted to the analysis by the HPLC system without any preseparation steps. In the first dimension separation cobalt was eluted as Co(II)-edta and was separated from trivalent metal complexes such as Fe(III) and Cr(III), while it was selectively oxidized to Co(III)-edta in the PGC column and then completely separated from the divalent metal complexes such as Ni(II) on the second column. A successful application of this method to accurate and precise determination of trace amount of cobalt in a stainless steel is demonstrated.
  IRON STEEL INST JAPAN KEIDANREN KAIKAN, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2355/isijinternational.52.1622
  DOI ID：10.2355/isijinternational.52.1622, ISSN：0915-1559, Web of Science ID：WOS:000309297800015
• Total Design of Novel Fluorescent Probes and Kinetically Integrated Chemical Systems for the Separation and Detection of Metal Ions　　　　　　　　　　　　　　　
  Shingo Saito
  BUNSEKI KAGAKU, 巻：60, 号：10, 開始ページ：773, 終了ページ：784, 2011年10月, ［査読有り］, ［筆頭著者, 責任著者］
  The total design of novel metal fluorescent probes for capillary electrophoresis-laser-induced fluorescence detection (CE-LIF), and along with a systematic study for high resolution by combination of various chemical parameters, including fluorometric properties, thermodynamics, and kinetics, were conducted. In these studies, sub-ppt sensitivity levels, high resolution among metal probe complexes, and robust applications to real samples were successfully obtained. In probe design, the quenching effect by heavy and paramagnetic metal ions was effectively controlled by adjusting the distance between the fluorophore and chelating moiety. Chelating moieties suitable to yield kinetic inertness were investigated amongst acyclic or macrocyclic hexa- or octadentates. In addition to the probe design, two new separation modes were developed, including : (i) a dynamic ternary complexing separation mode, in which the charge of an inert mother metal-probe complex is controlled by adding auxiliary ligands to form a ternary complex; (ii) an ion association complex separation mode, in which the addition of a polycation gives different sizes or shapes of polycation-probe association complexes, which, in turn, lead to different mobilities. Furthermore, a chemical suppression technique of metal-ion contaminants was developed, since the problem of contamination is of great importance for ultratrace detection. The contamination suppression system was achieved by integrating kinetics, and thermodynamics of the probe and the suppression reagent complexes with metal ions. This system successfully provided a contamination-free and -stable system for ppt level detection.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.60.773
  DOI ID：10.2116/bunsekikagaku.60.773, ISSN：0525-1931, Web of Science ID：WOS:000296316500001
• Reversed-phase ion-pair liquid chromatographic method for determination of reaction equilibria involving ionic species: Exemplification of the method using ligand substitution reactions of ethylenediaminetetraacetatochromium(III) ion with acetate and phosphate ions　　　　　　　　　　　　　　　
  Emiko Sato; Seiko Miya; Kazunori Saitoh; Shingo Saito; Masami Shibukawa
  JOURNAL OF CHROMATOGRAPHY A, 巻：1218, 号：7, 開始ページ：922, 終了ページ：928, 2011年02月, ［査読有り］
  A reversed-phase ion-pair liquid chromatographic method is presented for the determination of reaction equilibria involving ionic species of the same charge sign as reactant and product compounds. It has been demonstrated that ion-exchange chromatography or reversed-phase ion-pair chromatography is a useful tool for the determination of equilibrium constants of chemical reactions involving ionic species such as metal complexation reactions. Previous work with these methods has been based on the assumption that the limiting retention factors of the reactant and product species are constant independent of concentration of the chemical species (X) in the mobile phase, which reacts with the analyte compound. However, when all the reactant and product species are ions of the same charge sign as that of the species X, it is virtually impossible to apply these methods to the equilibrium constant determination because the retention factors of both the reactant and product species may depend on the concentration of X. In this study, an alternative approach was developed that estimates the limiting retention factors of ionic species from the dependence of the retention factor on the ionic strength of the mobile phase. Ligand substitution reactions of ethylenediaminetetraacetatochromium(III) ion with acetate and phosphate ions were used as model reactions to test this method. The equilibrium constants determined by this method are in good agreement with those obtained by a UV-visible spectrophotometric method. (C) 2010 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2010.12.063
  DOI ID：10.1016/j.chroma.2010.12.063, ISSN：0021-9673, Web of Science ID：WOS:000287281200006
• Capillary electrophoresis with laser-induced fluorescent detection method using highly emissive probes for analysis of actinides in radioactive wastes　　　　　　　　　　　　　　　
  Tomoko Haraga; Yuta Nakano; Masami Shibukawa; Yutaka Kameo; Kuniaki Takahashi; Shingo Saito
  Proceedings of the International Conference on Radioactive Waste Management and Environmental Remediation, ICEM, 開始ページ：1461, 終了ページ：1465, 2011年, ［査読有り］, ［責任著者］
  Actinides are important nuclides for the analysis of radioactive wastes arising from nuclear fuel cycle facilities. In order to achieve simple and rapid analysis of actinides, capillary electrophoresis-laser-induced fluorescent detection method (CE-LIF) is one of the potential candidates. In this study, new emissive probes of actinide ions suitable for CE-LIF were developed for the first time. The detection and separation of americium and neptunium ions as model nuclides were examined using several new emissive complexing probes, each of which possessed a fluorophore and a different chelating moiety. With a pre-capillary complexation technique without addition of the probe to separation buffer electrolyte, the highly sensitive fluorescent detection of Am and Np was successfully achieved using acyclic and macrocyclic multidentate probes. The results suggests that the probe with an acyclic hexadentate chelating moiety is suitable for detection and separation of Am and Np. The detection limit of mid-ppt levels was determined. Copyright © 2011 by ASME.
  英語, 研究論文（国際会議プロシーディングス）
  DOI：https://doi.org/10.1115/ICEM2011-59092
  DOI ID：10.1115/ICEM2011-59092, SCOPUS ID：84882942012
• Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers　　　　　　　　　　　　　　　
  Shingo Saito; Yuta Nakano; Atsushi Hikichi; Ryouji Suzuki; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama; Masami Shibukawa
  ANALYST, 巻：136, 号：13, 開始ページ：2697, 終了ページ：2705, 2011年, ［査読有り］, ［筆頭著者, 責任著者］
  Novel fluorescent probes have been developed for the ultratrace detection of heavy metal ions by capillary electrophoresis using laser-induced fluorescence detection. Based on a molecular design, the probes are composed of an octadentate chelating moiety, a macrocyclic DOTA (tetraazacyclododecanetetraacetic acid) and an acyclic DTPA (diethylenetriaminepentaacetic acid) frame, a spacer and a fluorophore (fluorescein). These were chosen on the basis of their ability to form kinetically inert and highly emissive complexes, and to prevent a quenching effect even with heavy and paramagnetic metal ions. Addition of a cationic polymer, polybrene, in the separation buffer provided high resolution and simultaneous detection of Ca(2+), Mg(2+), Cu(2+), Zn(2+), Ni(2+), Co(2+), Mn(2+), Cd(2+) and Pb(2+). The direct fluorescence detection of these metal ions with high sensitivity at lower ppt levels, typically 2-7 x 10(-11) M (potentially sub-ppt), was successfully achieved. While separation of anionic compounds using a counter cation ("Ion Association (IA)" mode) is typically controlled by the ion association equilibrium constants, K(ass), it was found that differences in the mobilities, mu(CP(IAC)), of the ion association complexes formed between the probe complexes and counter cations are the driving forces for separation in this new method. This suggests that each of the polybrene-probe complexes has different chemical structures among metal ions, which were able to be determined by CD spectra in this investigation. This novel separation mode was termed the "Ion Association Complex (IAC)" mode, distinct from the IA mode.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c0an00777c
  DOI ID：10.1039/c0an00777c, ISSN：0003-2654, eISSN：1364-5528, Web of Science ID：WOS:000291611800004
• Interfacial water on hydrophobic surfaces recognized by ions and molecules　　　　　　　　　　　　　　　
  Masami Shibukawa; Yoshiki Kondo; Yawara Ogiyama; Keita Osuga; Shingo Saito
  PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 巻：13, 号：35, 開始ページ：15925, 終了ページ：15935, 2011年, ［査読有り］
  Recent spectrophotometric and molecular dynamics simulation studies have shown that the physicochemical properties and structures of water in the vicinity of hydrophobic surfaces differ from those of the bulk water. However, the interfacial water acting as a separation medium on hydrophobic surfaces has never been detected and quantified experimentally. In this study, we show that small inorganic ions and organic molecules differentiate the interfacial water formed on the surfaces of octadecyl-bonded (C-18) silica particles from the bulk water and the chemical separation of these solutes in aqueous media with hydrophobic materials can be interpreted with a consistent mechanism, partition between the bulk water phase and the interfacial water formed on the hydrophobic surface. Thermal transition behaviour of the interfacial water incorporated in the nanopores of the C-18 silica materials and the solubility parameter of the water calculated from the distribution coefficients of organic compounds have indicated that the interfacial water may have a structure of disrupted hydrogen bonding. The thickness of the interfacial water or the limit of distance from the hydrophobic surface at which molecules and ions can sense the surface was estimated to be 1.25 +/- 0.13 nm from the volume of the interfacial water obtained by a liquid chromatographic method and the surface area, suggesting that the hydrophobic effect may extend beyond the first solvation shell of water molecules directly surrounding the surfaces.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/c1cp20704k
  DOI ID：10.1039/c1cp20704k, ISSN：1463-9076, eISSN：1463-9084, Web of Science ID：WOS:000294167700024
• Removal of Cr(VI) from aqueous solutions by the culm of bamboo grass treated with concentrated sulfuric acid　　　　　　　　　　　　　　　
  M. Koroki; S. Saito; H. Hashimoto; T. Yamada; Masakazu Aoyama
  ENVIRONMENTAL CHEMISTRY LETTERS, 巻：8, 号：1, 開始ページ：59, 終了ページ：61, 2010年03月, ［査読有り］
  We prepared a carbonaceous sorbent for Cr(VI) from the culm of Sasa kurilensis by dehydration with concentrated H(2)SO(4). The removal of Cr(VI) by the sorbent was highly solution pH dependent and mainly governed by physicochemical sorption. The equilibrium data fit well in the Langmuir isotherm model and indicate the endothermic nature of the Cr(VI) sorption. The desorption experiments suggest that the Cr(VI) sorption is generally irreversible, owing to strong interaction of HCrO(4)(-) with the active sites of the sorbent.
  SPRINGER HEIDELBERG, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s10311-008-0191-9
  DOI ID：10.1007/s10311-008-0191-9, ISSN：1610-3653, Web of Science ID：WOS:000275166200008
• Simple spectrophotometric determination of trace amounts of zinc in environmental water samples using aqueous biphasic extraction
  Masami Shibukawa; Daisuke Shirota; Shingo Saito; Shin Nagasawa; Kazunori Saitoh; Hiroaki Minamisawa
  Bunseki Kagaku, 巻：59, 号：10, 開始ページ：847, 終了ページ：854, 2010年
  A simple and highly selective and sensitive extraction spectrophotometric method is presented for the determination of trace amounts of zinc in environmental water samples. The method is based on the separation and preconcentration of zinc as its thiocyanate complex by aqueous biphasic extraction and subsequent determination by spectrophotometry. As a chromogenic reagent for the spectrophotometric determination of zinc, 5-(5-Bromo-2-pyridylazo)-5-[N-npropyl-N-(3-sulfopropyl)amino]-phenol (5-Br-PAPS) was used. Aqueous biphasic extraction can be performed by dissolving polyethylene glycol and sodium sulfate in a water sample. A rapid phase separation technique for this aqueous biphasic system was developed using thermal convection so that the extraction of zinc can be accomplished within 7 minutes without mechanical centrifugation. The calibration curve is linear in the concentration range of 0 ~ 200 μg L -1 and the determination limit calculated with ten-times the standard deviation of the reagent blank value is 1.6 μg L -1. Interferences from foreign metal ions were removed by separation with aqueous biphasic extraction and masking with suitable reagents for individual metal ions. The proposed method was applied to the determination of zinc in tap water and river water samples. The obtained results were in good agreement with those obtained by inductively coupled plasma atomic emission spectrometry. © 2010 The Japan Society for Analytical Chemistry.
  日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.59.847
  DOI ID：10.2116/bunsekikagaku.59.847, ISSN：0525-1931, SCOPUS ID：78650457630
• Superheated Water Ion-Exchange Chromatography: An Experimental Approach for Interpretation of Separation Selectivity in Ion-Exchange Processes　　　　　　　　　　　　　　　
  Masami Shibukawa; Tomomi Shimasaki; Shingo Saito; Takashi Yarita
  ANALYTICAL CHEMISTRY, 巻：81, 号：19, 開始ページ：8025, 終了ページ：8032, 2009年10月
  Cation-exchange selectivity for alkali and alkaline-earth metal ions and tetraalkylammonium ions on a strongly acidic sulfonic acid cation-exchange resin has been investigated in the temperature range of 40-175 degrees C using superheated water chromatography. Dependence of the distribution coefficient (In K(D)) on the reciprocal of temperature (1/T) is not linear for most of the ions studied, and the selectivity coefficient for a pair of alkali metal ions or that of alkaline-earth metal ions approaches unity as temperature increases. On the other hand, the retention order of tetraalkylammonium ions is reversed at 1.60 degrees C or above when eluted with Na(2)SO(4) aqueous solution and the larger ions are eluted faster than the smaller ones contrary to the retention order obtained at ambient temperature. The change in ion-exchange selectivity with temperature observed with superheated water chromatography has been discussed on the basis of the effect of temperature on hydration of the ions and specific adsorption or distribution of ionic species between the external solution and ion-exchange resin. In superheated water, the electrostatic interaction or association of the ions with the fixed ion becomes a predominant mechanism resulting in different separation selectivity from that obtained at ambient temperature.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac9011864
  DOI ID：10.1021/ac9011864, ISSN：0003-2700, CiNii Articles ID：80020661337, PubMed ID：19743878, Web of Science ID：WOS:000270361100026
• Superheated Water Ion-Exchange Chromatography: An Experimental Approach for Interpretation of Separation Selectivity in Ion-Exchange Processes　　　　　　　　　　　　　　　
  Masami Shibukawa; Tomomi Shimasaki; Shingo Saito; Takashi Yarita
  ANALYTICAL CHEMISTRY, 巻：81, 号：19, 開始ページ：8025, 終了ページ：8032, 2009年10月
  Cation-exchange selectivity for alkali and alkaline-earth metal ions and tetraalkylammonium ions on a strongly acidic sulfonic acid cation-exchange resin has been investigated in the temperature range of 40-175 degrees C using superheated water chromatography. Dependence of the distribution coefficient (In K(D)) on the reciprocal of temperature (1/T) is not linear for most of the ions studied, and the selectivity coefficient for a pair of alkali metal ions or that of alkaline-earth metal ions approaches unity as temperature increases. On the other hand, the retention order of tetraalkylammonium ions is reversed at 1.60 degrees C or above when eluted with Na(2)SO(4) aqueous solution and the larger ions are eluted faster than the smaller ones contrary to the retention order obtained at ambient temperature. The change in ion-exchange selectivity with temperature observed with superheated water chromatography has been discussed on the basis of the effect of temperature on hydration of the ions and specific adsorption or distribution of ionic species between the external solution and ion-exchange resin. In superheated water, the electrostatic interaction or association of the ions with the fixed ion becomes a predominant mechanism resulting in different separation selectivity from that obtained at ambient temperature.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac9011864
  DOI ID：10.1021/ac9011864, ISSN：0003-2700, Web of Science ID：WOS:000270361100026
• Recognition of Monosaccharides with Energy-transfer Luminescence Using Residual Coordination Sites of Lanthanide(III)-4-Aminobenzyl-EDTA Complex in Aqueous Solution　　　　　　　　　　　　　　　
  Shingo Saito; Atsushi Hikichi; Takao Kamura; Kazuyuki Hattori; Masakazu Aoyama; Masami Shibukawa
  CHEMISTRY LETTERS, 巻：38, 号：5, 開始ページ：412, 終了ページ：413, 2009年05月, ［筆頭著者, 責任著者］
  In this paper, we present the luminescent lanthanide complexes, Tb3+- and Dy3+-4-aminobenzyl-EDTA as receptors for monosaccharides. The monosaccharides are recognized through their coordinate bonds and hydration bonds in alkaline aqueous solution by distinctive energy-transfer luminescence enhancement of the center metal ions. The stability constants of the ternary complexes formed with several monosaccharides, which are particularly sensitive in the case of N-acetylneuraminic acid (Neu5Ac), were measured. A high stability constant for Neu5Ac was observed with an affinity of about 5000.
  CHEMICAL SOC JAPAN, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1246/cl.2009.412
  DOI ID：10.1246/cl.2009.412, ISSN：0366-7022, eISSN：1348-0715, CiNii Articles ID：10025121487, Web of Science ID：WOS:000267362100012
• Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex　　　　　　　　　　　　　　　
  Hiroshi Atsumi; Keitaro Yoshimoto; Shingo Saito; Moriya Ohkuma; Mizuo Maeda; Yukio Nagasaki
  TETRAHEDRON LETTERS, 巻：50, 号：19, 開始ページ：2177, 終了ページ：2180, 2009年05月
  We newly synthesized a nucleobase-binding ligand, ND-DOTA, in which 2-amino-5,7-dimethyl-1,8-naphthyridine (ND) was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (ND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb(3+). The blue emission of ND-DOTA was selectively quenched by adding abasic site-containing DNA duplexes that have pyrimidine bases opposite to the abasic site. In contrast, at the same excitation wavelength, ND-DOTA-Tb showed green emission independently of the bases opposite to the abasic site. Thus, a mixed solution of ND-DOTA and ND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength. (C) 2009 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.tetlet.2009.02.152
  DOI ID：10.1016/j.tetlet.2009.02.152, ISSN：0040-4039, eISSN：1873-3581, CiNii Articles ID：120001300353, Web of Science ID：WOS:000265136000004
• Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex　　　　　　　　　　　　　　　
  Hiroshi Atsumi; Keitaro Yoshimoto; Shingo Saito; Moriya Ohkuma; Mizuo Maeda; Yukio Nagasaki
  TETRAHEDRON LETTERS, 巻：50, 号：19, 開始ページ：2177, 終了ページ：2180, 2009年05月, ［査読有り］
  We newly synthesized a nucleobase-binding ligand, ND-DOTA, in which 2-amino-5,7-dimethyl-1,8-naphthyridine (ND) was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (ND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb(3+). The blue emission of ND-DOTA was selectively quenched by adding abasic site-containing DNA duplexes that have pyrimidine bases opposite to the abasic site. In contrast, at the same excitation wavelength, ND-DOTA-Tb showed green emission independently of the bases opposite to the abasic site. Thus, a mixed solution of ND-DOTA and ND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength. (C) 2009 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.tetlet.2009.02.152
  DOI ID：10.1016/j.tetlet.2009.02.152, ISSN：0040-4039, eISSN：1873-3581, Web of Science ID：WOS:000265136000004
• Separation selectivity of aqueous polyethylene glycol-based separation systems: DSC, LC and aqueous two-phase extraction studies　　　　　　　　　　　　　　　
  Masami Shibukawa; Ryoichi Ichikawa; Takayuki Baba; Ryosaku Sakamoto; Shingo Saito; Koichi Oguma
  POLYMER, 巻：49, 号：19, 開始ページ：4168, 終了ページ：4173, 2008年09月
  The distribution behavior of n-alcohols, ketones and nitroalkanes in aqueous liquid chromatography with a column packed with polyethylene glycol (PEG) gel, TSKgel Ether-250, was compared with that in aqueous two-phase systems (ATPSs) formed from PEG and Na2SO4 or (NH4)(2)SO4. The plots of the distribution data obtained for the PEG gel system against those for the ATPS reveal that the separation selectivities exerted by the PEG gel system and the PEG-based ATPS are approximately the same. Differential scanning calorimetry studies on aqueous PEG solutions suggest that PEG polymer forms a hydration structure of which the composition is 50% (w/w) PEG or the hydration number per ethylene oxide is 2.4 and the separation selectivity of the PEG-water systems can be attributed to partition of solute compounds into the hydrated PEG polymer structure. (C) 2008 Elsevier Ltd. All rights reserved.
  ELSEVIER SCI LTD, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.polymer.2008.07.042
  DOI ID：10.1016/j.polymer.2008.07.042, ISSN：0032-3861, eISSN：1873-2291, CiNii Articles ID：80019775078, Web of Science ID：WOS:000259303900019
• Control of the contaminant level for determination of Al3+ using 8-quinolinol by high-performance liquid chromatography with fluorescence detection　　　　　　　　　　　　　　　
  Shingo Saito; Jun-ichi Tamura; Makoto Sato; Masakazu Aoyama
  JOURNAL OF CHROMATOGRAPHY A, 巻：1190, 号：1-2, 開始ページ：198, 終了ページ：203, 2008年05月
  We developed a technique controlling contaminant Al3+ level using a combination of kinetics and thermodynamics at a pre-column derivatizing step in HPLC. The technique involves modifying a conventional HPLC of 8-quinolinol (Ox) complex with Al3+. The contaminant suppressing reagents, dihydroxyazobenzene (DHAB) and 1,2,3-trihydroxybenzene (pyrogallol) were added to the Ox and pH buffer solutions to convert contaminant Al3+ in these solutions to inactive complexes. The Al3+ in the sample selectively formed Ox complexes with fast kinetics. After that, the labeled fluorescent complexes in the resultant metastable state were separated in the HPLC. This technique successfully suppressed contamination by a factor of 17. This method allowed for an improvement in the detection limits and also provided a stable blank. (C) 2008 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2008.03.018
  DOI ID：10.1016/j.chroma.2008.03.018, ISSN：0021-9673, CiNii Articles ID：80019615886, Web of Science ID：WOS:000255848600024
• Solubilization of polystyrene into monoterpenes　　　　　　　　　　　　　　　
  Kazuyuki Hattori; Satofumi Naito; Kenji Yamauchi; Hisayuki Nakatani; Takashi Yoshida; Shingo Saito; Masakazu Aoyama; Tetsuo Miyakoshi
  Advances in Polymer Technology, 巻：27, 号：1, 開始ページ：35, 終了ページ：39, 2008年
  The ability of certain monoterpenes from the essential oils of Abies sachalinensis and Eucalyptus species to dissolve polystyrene (PS) was studied. These two essential oils themselves were also examihed. The aim of this study is to recycle PS without a melting process and without using petroleum-derived solvents. The relationship between chemical structure of the terpenes and their dissolving power for PS is investigated through the solubility parameter and apparent activation energy for dissolution. α-Terpinene and its positional isomers on a C=C bond have high solvent powers for PS, whereas bicyclic terpenes are inferior in this regard even though they have similar solubility parameters to that of α-terpinene. It is suggested that the bulky and/or hydrophilic structures in the solvent molecule prevent the dissolution of PS. Simple steam distillation of solutions of PS in the terpenes gave recovery of more than 97% of the PS and terpenes. The present solvent systems cause little degradation to PS and are promising for the recycling of PS using sustainable solvents. © 2008 Wiley Periodicals, Inc.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/adv.20115
  DOI ID：10.1002/adv.20115, ISSN：0730-6679, CiNii Articles ID：80020036030, SCOPUS ID：57549113705
• Inhibitory effects of L-pipecolic acid from the edible mushroom, Sarcodon aspratus, on angiotensin I-converting enzyme　　　　　　　　　　　　　　　
  Masaaki Kiyoto; Shingo Saito; Kazuyuki Hattori; Nam-Seok Cho; Takaaki Hara; Yuzo Yagi; Masakazu Aoyama
  JOURNAL OF WOOD SCIENCE, 巻：54, 号：2, 開始ページ：179, 終了ページ：181, 2008年
  The aqueous extract of the edible mushroom Sarcodon aspratus showed inhibitory effects against angiotensin I-converting enzyme (ACE). L-Pipecolic acid (L-piperidine-2-carboxylic acid) was isolated from a hot-water extract in a 0.02% yield as an active principle. The mode of inhibition of L-pipecolic acid was found to be competitive, whereas its D-isomer showed no significant inhibitory effects against ACE, suggesting that the configuration of the carboxyl group in the molecule plays an important role in the enzyme inhibition.
  SPRINGER TOKYO, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s10086-007-0923-7
  DOI ID：10.1007/s10086-007-0923-7, ISSN：1435-0211, Web of Science ID：WOS:000256405100014
• Removal of Cr(VI) by the bark of Quercus dentate treated with concentrated sulfuric acid　　　　　　　　　　　　　　　
  Motoki Koroki; Yukihiro Hashimoto; Shingo Saito; Tetuo Yamada; Harumi Hashimoto; Masakazu Aoyama
  Wood Carbonization Research, 巻：4, 号：2, 開始ページ：55, 終了ページ：61, 2008年
• Sorption of Cr(VI) on the wood of Japanese larch treated with concentrated sulfuric acid　　　　　　　　　　　　　　　
  Masakazu Aoyama; Shingo Saito; Masaaki Tagami
  JOURNAL OF WOOD SCIENCE, 巻：53, 号：6, 開始ページ：545, 終了ページ：549, 2007年12月
  A carbonaceous sorbent was prepared from the wood of Japanese larch (Larix leptolepis) by dehydration with concentrated sulfuric acid in a 69% yield. The abilities of the sorbent to remove Cr(VI) from aqueous solutions were investigated. Research parameters included the initial solution pH, temperature, and initial concentration of Cr(VI) in solution. The removal of Cr(VI) was highly solution pH dependent and was mainly governed by physicochemical sorption under weak acidic conditions. The equilibrium data fit well in the Langmuir isotherm model. The Langmuir constants were calculated at different temperatures, and the sorption capacity increased with rising temperature, indicating the endothermic nature of the Cr(VI) sorption onto the sorbent. The desorption experiments suggest that the Cr(VI) sorption is generally irreversible, owing to strong interaction of HCrO4- with the active sites of the sorbent.
  SPRINGER TOKYO, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s10086-007-0898-4
  DOI ID：10.1007/s10086-007-0898-4, ISSN：1435-0211, CiNii Articles ID：10022025247, Web of Science ID：WOS:000253284300015
• Direct fluorometric detection of paramagnetic and heavy metal ions at sub-amol level using an aromatic polyaminocarboxylate by CZE: Combination of pre- and on-capillary complexation technique　　　　　　　　　　　　　　　
  Shingo Saito; Ryouji Suzuki; Naokj Danzaka; Atsushi Hikichi; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
  ELECTROPHORESIS, 巻：28, 号：14, 開始ページ：2448, 終了ページ：2457, 2007年07月
  The low sensitivity of simple CZE for detecting metal ions is a long-standing problem even when an LIF detection system is employed. We have successfully achieved an ultrasensitive CE-LIF using a simple CZE mode (typical detection limit: 10(-11)-10(-10) mol/dm(3)). Both the design of a newly synthesized ligand and the combination of a precapillary derivatizing technique with an on-capillary ternary complexing technique have enabled us to achieve this extremely low LOD and high resolution of large metal complexes. The direct fluorescent detection of the paramagnetic metal ions was achieved for the first time despite their intrinsic fluorescent quenching nature. The fluorescent ligand (L) consists of a poly-aminocarboxylate chelating moiety, a strongly emissive fluorescein moiety and a spacer connecting the two portions. The migration behavior of various metal-L complexes was investigated. The resolution among the complexes was improved by the introduction of a ternary complex equilibrium of the kinetically stable mother complexes with OH-ion. The analytical potential of our simple system was examined, and it was proved that the system was one of the most sensitive methods without the need for any preconcentration process.
  WILEY-V C H VERLAG GMBH, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/elps.200600516
  DOI ID：10.1002/elps.200600516, ISSN：0173-0835, eISSN：1522-2683, CiNii Articles ID：120001370544, PubMed ID：17577884, Web of Science ID：WOS:000248390900016
• Fluorescence-based affinity labeling of nucleobase by hydrogen-bond forming metal complex　　　　　　　　　　　　　　　
  Keitaro Yoshimoto; Hiroshi Atsumi; Shingo Saito; Moriya Okuma; Mizuo Maeda; Yukio Nagasaki
  Nucleic Acids Symp. Ser., 巻：51, 開始ページ：303, 終了ページ：304, 2007年
• Synthesis of bis(alk-3-en-1-ynyl)benzene with either E- or Z-configuration via a one-pot three-component coupling reaction and its optical properties　　　　　　　　　　　　　　　
  Masayuki Hoshi; Souichi Suzuki; Shingo Saitoh; Mitsuhiro Okimoto; Kazuya Shirakawa
  TETRAHEDRON LETTERS, 巻：48, 号：1, 開始ページ：119, 終了ページ：124, 2007年01月
  A convenient, efficient and stereoselective synthesis of a range of bis(alk-3-en-1-ynyl)benzenes with E- and Z-configuration is described. The protocol involves Cu-mediated cross-coupling reaction of (E)- and (Z)-alk-1-enyldisiamylboranes with (trimethylsilyl)ethynyl bromide and Pd/Cu-catalyzed cross-coupling reaction with diiodobenzene. The sequential cross-coupling reaction proceeds readily under extremely mild conditions in a one-pot manner to afford bis(alk-3-en-1-ynyl) benzenes in good to excellent yields. The optical properties of the products are also described. (c) 2006 Elsevier Ltd. All rights reserved.
  PERGAMON-ELSEVIER SCIENCE LTD, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.tetlet.2006.10.162
  DOI ID：10.1016/j.tetlet.2006.10.162, ISSN：0040-4039, CiNii Articles ID：80018734376, Web of Science ID：WOS:000243630300025
• Antihypertensive Components in the Fruit Body of Ganoderma lucidum　　　　　　　　　　　　　　　
  Masakazu Aoyama; Shingo Saito; Masaaki Kiyoto; Iwao Togashi; Takaaki Hara; Yuzo Yagi; Teruhisa Kimura; Shozo Yoneyama
  Mushroom Sci. Biotechnol., 巻：15, 号：2, 開始ページ：91, 終了ページ：96, 2007年
  DOI：https://doi.org/10.24465/msb.15.2_91
  DOI ID：10.24465/msb.15.2_91
• Direct fluorescence detection of ultratrace lanthanide(III) ions complexed with aromatic polyaminocarboxylate, avoiding quenching of ligand-centered emission, using capillary zone electrophoresis with a ternary complexing technique　　　　　　　　　　　　　　　
  Shingo Saito; Shingo Takeuchi; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
  ANALYST, 巻：132, 号：3, 開始ページ：237, 終了ページ：241, 2007年
  The ultratrace level detection and the separation of lanthanide ions (Ln(3+)) were achieved using capillary zone electrophoresis with laser induced fluorescent detection (CZE-LIF) using an aromatic polyaminocarboxylate ligand synthesized in our previous work. The ligand forms kinetically stable Ln complexes at the pre-capillary derivatizing step. It effectively avoids quenching processes of the ligand-centered fluorescence through complexation with Ln(3+) without paramagnetic and heavy atom effects because of the distance between the chelating and the antenna moieties. During the on-capillary separation step, the mother Ln complexes competitively form ternary complexes with the auxiliary ligands, iminodiacetate and citrate, which provide different mobilities for each of the Ln(3+) complexes. The emissively labeled Ln(3+) complexes were efficiently separated, based on the ternary complex equilibrium. Since the carrier buffer employed was free from emissive ligands, a high signal to noise ratio was obtained. A lower detection limit of 9.1 x 10(-11) mol dm(-3) (15.6 ng dm(-3), 0.46 attomole as an amount basis) was successfully achieved typically for Lu(3+) with a simple CZE mode. We propose a combination of a pre-capillary and an on-capillary complexing technique as a method that provides both high sensitivity and high resolution.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/b613534j
  DOI ID：10.1039/b613534j, ISSN：0003-2654, eISSN：1364-5528, CiNii Articles ID：120001370745, PubMed ID：17325757, Web of Science ID：WOS:000244487700009
• Selective ultratrace detection of Al(III) and Ga(III) complexed with a calcein isomer by capillary zone electrophoresis with laser-induced fluorescence detection - Comparison of emissive polyaminocarboxylates as derivatizing ligands　　　　　　　　　　　　　　　
  Shingo Saito; Jun-ichi Shimidzu; Keitaro Yoshimoto; Mizuo Maeda; Masakazu Aoyama
  JOURNAL OF CHROMATOGRAPHY A, 巻：1140, 号：1-2, 開始ページ：230, 終了ページ：235, 2007年01月
  A highly sensitive and selective capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF) for Al(3+) and Ga(3+) was developed using a calcein 4',5'-isomer as a pre-capillary complexing agent. Direct fluorescent detection without an agent added to the carrier buffer provided the notable detection limits of 3.9 x 10(-10) and 4.6 x 10(-10) mol dm(-3) for Al and Ga, respectively. By applying this system successfully to a river water sample, its potential for practical applications was ascertained. A comparison of three aromatic polyaminocarboxylate ligands related to the coordination structure and the distance between the fluorophore and the chelating moiety, revealed some interesting factors regarding their selectivity. (c) 2006 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2006.12.001
  DOI ID：10.1016/j.chroma.2006.12.001, ISSN：0021-9673, CiNii Articles ID：120000786948, PubMed ID：17178417, Web of Science ID：WOS:000243953300032
• Fluorescent-based SNP typing using hydrogen bond-forming small ligand and its lanthanide(III) complex　　　　　　　　　　　　　　　
  Keitaxo Yoshimoto; Hiroshi Atsumi; Seiichi Nishizawa; Shingo Saito; Hiroyuki Koshino; Moriya Okuma; Mizuo Maeda; Norio Teramae; Yukio Nagasaki
  PROGRESS ON POST-GENOME TECHNOLOGIES, 開始ページ：22, 終了ページ：23, 2007年, ［査読有り］
  PHOENIX PUBL & MEDIA NETWORK, 英語, 研究論文（国際会議プロシーディングス）
  Web of Science ID：WOS:000251162700016
• Highly sensitive determination of lanthanides by capillary electrophoresis with direct visible detection after precapillary complexation with aromatic polyaminocarboxylate and additionally applying dynamic ternary complexation with nitrilotriacetic acid　　　　　　　　　　　　　　　
  Shingo Saito; Naoki Danzaka; Suwaru Hoshi
  ELECTROPHORESIS, 巻：27, 号：15, 開始ページ：3093, 終了ページ：3100, 2006年08月
  A newly synthesized aromatic polyarninocarboxylate (NBD-ABEDTA, H4L) was applied to precapillary derivatizing capillary electrophoresis as a chelating reagent for lanthanide ions (Ln(3+)). The Ln-L complexes provide both kinetic stability on dissociation due to their methyl-EDTA coordinating structure, and high light absorptivity (epsilon(max) = 2.4 x 10(4) cm(-1) mol(-1) dm(3)) in the visible region at 469 nm thanks to their nitrobenzofurazan moiety. A ligand was employed for capillary zone electrophoresis based on a unique concept: both precapillary and dynamic on-capillary complexation were carried out on one center-metal ion to achieve high resolution. As a ternary complex-formation agent, iminodiacetate (IDA), bound to the mother complex (Ln-L), was added to the carrier buffer solution. The carrier buffer solution of 9.5 mmol(.)dm(-3) (pH 9.45) borate and 33.5 mmol(.)dm(-3) IDA, drastically improved the resolution among Ln(3+) ions. Each of the Ln complexes was effectively separated, except for Pr-Sm. Furthermore, the absence of L from the carrier solution, which stabilizes the baseline fluctuation, provided low LOD (typically 4.2 x 10(-7) mol(.)dm(-3)). This strongly suggests that Ln-L complexes are kinetically stable even with a large excess of IDA. Quite unexpectedly, the order of migration differs from that of the atomic number, inverting at Nd. This is due to the effect of the cavity size of the residual coordination sites on the ternary complexation and the electronic density of Ln(3+).
  WILEY-V C H VERLAG GMBH, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1002/elps.200500828
  DOI ID：10.1002/elps.200500828, ISSN：0173-0835, CiNii Articles ID：120001370629, PubMed ID：16800028, Web of Science ID：WOS:000239912600012
• Direct fluorescence detection of Pb_2^+ and Cd_2^+ by high performance liquid chromatography using 1-(4-Aminobenzyl)ethylenediamine-N,N,N’,N’-tetraacetate as a pre-column derivatizing agent　　　　　　　　　　　　　　　
  Shingo Saito; Naoki Danzaka; Suwaru Hoshi
  Journal of Chromatography A,, 巻：1104, 号：1-2, 開始ページ：140, 終了ページ：144, 2006年02月
  A highly sensitive HPLC with direct fluorescent detection (lambda(ex) = 235 nm, lambda(em) = 355 mn) was developed for Pb2+ and Cd2+ complexes with an aromatic polyaminocarboxylate, 1-(4-aminobenzyl)ethylenediantine-N,N,N',N'-tetraacetate as a pre-column derivatizing agent. A reversed phase partition column pretreated by a cationic surfactant was employed. Although this ligand forms thermodynamically stable complexes with various metal ions, only peaks of Ph2+ and Cd2+ were detected with the ligand-centered emission in the HPLC due to the emissive activity and kinetic stability in the dissociation reaction. The detection limits obtained were 1.5 x 10(-8) and 3.3 x 10(-9) mol l(-1) for Pb2+ and Cd2+, respectively. (c) 2005 Elsevier B.V. All rights reserved.
  ELSEVIER SCIENCE BV, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1016/j.chroma.2005.11.085
  DOI ID：10.1016/j.chroma.2005.11.085, ISSN：0021-9673, CiNii Articles ID：80017642843, PubMed ID：16343515, Web of Science ID：WOS:000235100300019
• Chemical suppression of contaminant metal ions using a metastable state in precolumn derivatizing HPLC: An ultratrace fluorometric detection of Al(III)　　　　　　　　　　　　　　　
  S Saito; T Anada; S Noshi; K Akatsuka
  ANALYTICAL CHEMISTRY, 巻：77, 号：16, 開始ページ：5332, 終了ページ：5338, 2005年08月
  The contamination of metal ions from reagents used frequently restricts the practical detection limit of the metal ion, which itself is a source of contamination. We have found a novel solution to this problem, a chemical-suppressing method of contaminant metal ions on a reversed-phase HPLC for Al3+ with a detection limit of 7.6 X 10(-11) mol dm(-3) (2.1 ng dm-3) by only adding a certain agent into all stock solutions without any preconcentration or purification steps. This technique decreases the concentration of the contaminant Al3+ originating from the reagents by more than 1 order of magnitude using selective derivatization of sample Al3+ ions to a powerful fluorescent complex at a metastable state in the precolumn chelation processes. Meanwhile, the contaminant Al3+ remains as a nonfluorescent complex with a blocking reagent in order to suppress the contamination. This selective derivatization is achieved by the accumulation of several complexation processes based on the difference of formation, dissociation, and ligand-exchange kinetics and the thermodynamics between the derivatizing reagent, the 4',5'-geometorical isomer of calcein, and the blocking reagent, o,o'-dihydroxyazobenzene. This simple and smart HPLC system was validated through recovery tests of environmental and biological samples.
  AMER CHEMICAL SOC, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ac050380c
  DOI ID：10.1021/ac050380c, ISSN：0003-2700, CiNii Articles ID：80017543888, PubMed ID：16097776, Web of Science ID：WOS:000231236300035
• Simultaneous detection of [metal(II)-tpen](2+) as kinetically inert cationic complexes using pre-capillary derivatization electrophoresis: an application to biological samples　　　　　　　　　　　　　　　
  S Saito; S Sasamura; S Hoshi
  ANALYST, 巻：130, 号：5, 開始ページ：659, 終了ページ：663, 2005年
  A high resolution of doubly charged first row transition (Fe, Cu, Zn, Ni, Co, Mn) and heavy metal (Pb, Cd, Hg) ions was achieved in capillary electrophoresis (CE) with high sensitivity (sub-micromol dm(-3) level), using NN,N' N'-tetrakis(2- pyridylmethyl)ethylenediamine ( TPEN) as a pre-capillary derivatizing agent. The non-charged reagent, TPEN, was applied to capillary zone electrophoresis (CZE) for the first time. Since complete spatial separation between the complexes and the ligand was carried out in a carrier buffer, which was free of TPEN, kinetic inertness of metal complexes was necessary for the detection in this pre-capillary method. All the nine listed metal complexes were detected: Ca2+, Mg2+, Al3+, Fe3+, and Co3+ complexes were undetectable. This, interestingly, suggests that those nine cations form kinetically inert tpen complexes without strong charge-charge interactions between the metal ion and the ligand. It is expected that the hard-soft-acid-base ( HSAB) principle governed the kinetics selectivity. With respect to the electrophoretic behavior, the addition of chloride ion and methanol to the carrier significantly improved the resolution. This is due to the formation of ternary complexes or ion aggregates and the solvation effect, respectively. These effects provided a satisfactory baseline resolution among the nine metal ions. An application to biological samples was demonstrated. Some metal ions in human serum and urine were successfully detected in a simple process without the need for deproteinization using a non-coated fused-silica capillary because of the differenciation in the direction of migration between organic matter and complexes.
  ROYAL SOC CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1039/b417394e
  DOI ID：10.1039/b417394e, ISSN：0003-2654, CiNii Articles ID：120001370814, PubMed ID：15852134, Web of Science ID：WOS:000228689600008
• Highly-sensitive simultaneous detection of lanthanide(III) ions as kinetically stable aromatic polyaminocarboxylato complexes via capillary electrophoresis using resolution enhancement with carbonate ion　　　　　　　　　　　　　　　
  S Saito; H Hoshino
  ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 巻：378, 号：6, 開始ページ：1644, 終了ページ：1647, 2004年03月, ［査読有り］
  An aromatic polyaminocarboxylate ligand, 1-(4-aminobenzyl)ethylenediamine-N,N,N'N'-tetraacetate (ABEDTA), is proposed as a complexing reagent in the pre-capillary mode so as to form kinetically inert Ln(III) complexes, meaning that no added ligand is necessary in alkaline carrier buffer solutions. In addition, highly-sensitive detection is possible through a light-absorbing moiety of an aminobenzyl group in the ligand. The fine-tuning of the electrophoretic mobilities of the Ln-abedta complexes is successfully achieved by adding an auxiliary carbonate ion ligand which alters the charge-to-size ratio of the complexes through fast exchange equilibria in a carrier buffer. In fact, all of the complexes are detectable with very similar analytical sensitivity and acceptable resolution (except for Ln=Sm, Eu, Gd) by using NaOH-borate carrier buffer solution at pH 12.35 with 20 mM of Na2CO3. A typical detection limit for Tb(III) ion (to 3sigma) is as low as 0.94 muM, which translates to an absolute amount of 9.4 fmol in a 1.0 x 10(-8) dm(-3) (10 nL) injection.
  SPRINGER-VERLAG HEIDELBERG, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1007/s00216-003-2454-4
  DOI ID：10.1007/s00216-003-2454-4, ISSN：1618-2642, PubMed ID：15214429, Web of Science ID：WOS:000220661800031
• Formation reactions and photophysical parameters of highly luminescent lanthanoids(III) complexes with 4-hydroxypyridine-2,6-dicarboxylic acid　　　　　　　　　　　　　　　
  N Takahashi; S Saito; H Hoshino
  BUNSEKI KAGAKU, 巻：52, 号：9, 開始ページ：713, 終了ページ：718, 2003年09月, ［査読有り］
  For the purpose of finding ligand designing strategy for lanthanoids energy-transfer luminescence, formation reactions of 4-hydroxypyridine-2,6-dicarboxylic acid (chelidamic acid, CDA) with terbium (111) and europium (111) ions in aqueous solution were investigated. A luminescent complex species having I : 3 stoichiometry was formed at pH 8.4 similar to 11.6. Those complexes were hydrolyzed by more alkaline solution. The relative quantum yield of Tb(III)-GDA complex at pH 11.6 was 0.636 (lambda(em) = 544 nm) on a quinine sulfate basis. The luminescence lifetimes of Tb(III)-GDA were calculated as 1.02 ms at pH 8.4 and 1.55 ms at pH 11.6. The luminescence intensities of Tb(III) and Eu(Ill)-CDA complexes increased from pH 9 to 11 with the deprotonation degree of the 4-hydroxyl group on the complexes. The deprotonated Ln(Ill)CDA complex species possibly has a resonance structure of the pyridone type, which would contribute to the highly enhanced luminescence intensity. Detailed photophysical data in terms of photoexcitation and the triplet energy level (low temperature phosphorescence) are given.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  ISSN：0525-1931, Web of Science ID：WOS:000185447700010
• 4-ヒドロキシピリジン-2,6-ジカルボン酸-ランタノイド（III）錯体の生成反応とエネルギー移動発光挙動　　　　　　　　　　　　　　　
  高橋 伸幸; 斎藤 伸吾; 星野 仁
  分析化学, 巻：52, 号：9, 開始ページ：713, 終了ページ：718, 2003年
  DOI：https://doi.org/10.2116/bunsekikagaku.52.713
  DOI ID：10.2116/bunsekikagaku.52.713, ISSN：0525-1931, CiNii Articles ID：110002905278
• 解離不活性ランタノイド－ポリアミノカルボン酸錯体を用いる高性能分析法の開発　　　　　　　　　　　　　　　
  齋藤伸吾
  分析化学, 巻：52, 号：1, 開始ページ：81, 終了ページ：82, 2003年
  Analytical methods of Ln^III were developed in which the kinetic inertness of Ln^III complexes with acyclic polyaminocarboxylate (PAC) on the dissociation reaction was the key to establishing detection systems. First, a pre-derivatization HPLC method with fluorescent detection was developed for closedshell Ln^III using an aromatic octadentate PAC as the derivatizing reagent. Selective detection at ppb levels was achieved. Second, the factors to form inert Ln-pac complexes were investigated on the dissociation reaction. The rate constants and activation parameters were determined for a series of Ln^III-hexadentate and octadentate PAC complexes. Interestingly, an unusually large negative activation entropy for solvolysis governed the kinetic inertness, and was probably derived from metal envelopment in the complex and hydration on the activation state. Third, a novel measurement method of the dissociation rate was developed using capillary electrophoresis (CE). The dissociation rate constant for the Ce^III-PAC complex was determined for the first time. Fourth, based on the result of the second study, a sensitive pre-derivatizing CE method for Ln^III at the fmol level was developed using an aromatic EDTA derivative. In this method, a high resolution was achieved by introducing a ternary complex equilibrium in the carrier. Finally, the energy-transfer luminescence of a Tb^III complex with a PAC possessing phenol function was studied. The detection limit at the ppt level was achieved, and the photophysics was also investigated.
  日本分析化学会, 日本語
  CiNii Articles ID：110002905162, CiNii Books ID：AN00222633
• Visual fluorimetry of trace lead using ion-pair extraction onto an octadecylsilanized silica disk　　　　　　　　　　　　　　　
  J Isoe; Y Sakai; E Kaneko; S Saito; S Hoshi; K Akatsuka
  BUNSEKI KAGAKU, 巻：51, 号：11, 開始ページ：1037, 終了ページ：1040, 2002年11月, ［査読有り］
  A rapid and simple method has been developed for the determination of trace lead ion using an octadecylsilanized (ODS) silica disk cartridge. The ion-pair of a lead complex with 5,614,15-dibenzo-4,7,13,16,21,24-hexaoxa-1.10- diazabicyclohexacosane (DBC 2.2.2) and tetrabromofluorescein (Eosin Y) was extracted from a sample solution onto an ODS filter at pH 7.0. It was found that the addition of 20 wt% ethanol to the sample solution significantly enhanced the solid phase extraction. The lead concentration was determined by visual fluorimetry under ultraviolet light in the dark. The ion-pair has excitation and emission maxima at 530 mn and 560 nm in the solid phase, respectively. When measured with a densitometer, the linear range of this method is up to 2.9 X 10(-8) mol dm(-3) (6 ppb). The detection limit is 5.0 X 10(-10) mol dm(-3) (100 ppt) by visual determination and 3.4 X 10(-9) mol dm(-3) (700 ppt) by densitometry. The relative standard deviation for 1.2 X 10(-3) mol dm(-3) (2.3 ppb) of lead is 5.6% (n = 7). The method is essentially free from matrix interference. The proposed method can easily be performed and provides good precision and accuracy.
  JAPAN SOC ANALYTICAL CHEMISTRY, 中国語, 研究論文（学術雑誌）
  ISSN：0525-1931, Web of Science ID：WOS:000179235800006
• Thin layer chromatographic behavior of a lanthanum-alizarin complexone-fluoride ternary complex　　　　　　　　　　　　　　　
  S Arasaka; E Kaneko; S Saito; S Hoshi; K Akatsuka
  BUNSEKI KAGAKU, 巻：51, 号：9, 開始ページ：837, 終了ページ：839, 2002年09月, ［査読有り］
  The thin-layer chromatographic behavior of a lanthanum-alizarin complexone (ALC)-fluoride complex was investigated with particular interest being paid to the separation of the ternary complex from La-ALC. Although the spectral shift of the La-ALC chelate upon binding to the fluoride ion is widely used for the spectrophotometric determination of fluoride ion, there do not appear to be any reports on thin-layer chromatography (TLC) for the ternary complex (La-ALC-F). In this study, it was found that when developed with 60% ethanol on a C-18-bonded silica thin-layer plate, the ternary complex was easily eluted, forming a sharp band. Conversely, the La-ALC chelate was immobilized at the spotting position as a fleck. Furthermore, when a bluish-purple band was separated from a reddish-purple fleck, the ternary complex faded in a few minutes, whereas, when the band remained inside the fleck, no fading took place. These results are interpreted in terms of the dissociation of the labile complex caused by separation from the excess reagent. Consequently, the concentration of the La-ALC-F band across the center of the fleck provides a sensitive visualization of the fluoride ion down to 0.1 ppm.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  ISSN：0525-1931, Web of Science ID：WOS:000178045100028
• Retention behavior of cationic aluminium chelate with o,o '-dihydroxyazobenzene in HPLC using the C-18-bonded silica stationary phase　　　　　　　　　　　　　　　
  T Kurasawa; T Yotsuyanagi; E Kaneko; S Saito; S Hoshi; K Akatsuka
  BUNSEKI KAGAKU, 巻：51, 号：9, 開始ページ：833, 終了ページ：836, 2002年09月, ［査読有り］
  The retention behavior of cationic aluminium chelate with o,o'-dihydroxyazobenzene (DHAB or H2L), [AIL](+), in high-performance liquid chromatography (HPLC) was investigated using the C-18-bonded silica stationary phase. In this study, it was found that when the pH of the mobile phase was 5.5, a significant tailing of the alminium chelate was observed, whereas when the pH was 2, a marked improvement of the elution was attained. These results were interpreted in terms of a silanol masking effect: when an acidic mobile phase is employed, protonation of the silanol groups on the surface is maintained, while eliminating the strong interaction between the cationic chelate and the stationary phase. The addition of sodium chloride in the eluent can also be used to eliminate peak tailing of the cationic chelate. Based on these results, the underlying immobilization mechanism of the aluminium chelate on the C-18-bonded silica thin layer is explained by an electrostatic interaction between the cationic aluminium chelate and deprotonated silanol groups on the solid surface. The example in this study should provide valuable information to improve the separation quality of cationic solutes in HPLC.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  ISSN：0525-1931, Web of Science ID：WOS:000178045100027
• オクタデシルシリル化シリカディスクを用いる微量鉛のイオン対抽出/蛍光目視定量　　　　　　　　　　　　　　　
  磯江 準一; 酒井 康裕; 金子 恵美子; 斎藤 伸吾; 星 座; 赤塚 邦彦
  分析化学, 巻：51, 号：11, 開始ページ：1037, 終了ページ：1040, 2002年
  A rapid and simple method has been developed for the determination of trace lead ion using an octadecylsilanized (ODS) silica disk cartridge. The ion-pair of a lead complex with 5,6-14,15-dibenzo-4,7,13,16,21,24-hexaoxa-1.10- diazabicyclohexacosane (DBC 2.2.2) and tetrabromofluorescein (Eosin Y) was extracted from a sample solution onto an ODS filter at pH 7.0. It was found that the addition of 20 wt% ethanol to the sample solution significantly enhanced the solid phase extraction. The lead concentration was determined by visual fluorimetry under ultraviolet light in the dark. The ion-pair has excitation and emission maxima at 530 nm and 560 nm in the solid phase, respectively. When measured with a densitometer, the linear range of this method is up to 2.9 × 10-8 mol dm-3 (6 ppb). The detection limit is 5.0 × 10-10 mol dm-3 (100 ppt) by visual determination and 3.4 × 10-9 mol dm-3 (700 ppt) by densitometry. The relative standard deviation for 1.2 × 10-3 mol dm -3 (2.3 ppb) of lead is 5.6% (n = 7). The method is essentially free from matrix interference. The proposed method can easily be performed and provides good precision and accuracy.
  Japan Society for Analytical Chemistry, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.51.1037
  DOI ID：10.2116/bunsekikagaku.51.1037, ISSN：0525-1931, SCOPUS ID：0036440272
• C_18修飾シリカ固定相を用いる高速液体クロマトグラフィーにおけるアルミニウム : ο,ο'-ジヒドロキシアゾベンゼン陽イオン性錯体の保持挙動　　　　　　　　　　　　　　　
  倉澤 隆; 四ツ柳 隆夫; 金子 恵美子; 斎藤 伸吾; 星 座; 赤塚 邦彦
  分析化学, 巻：51, 号：9, 開始ページ：833, 終了ページ：836, 2002年
  The retention behavior of cationic aluminium chelate with o,o′-dihydroxyazobenzene (DHAB or H2L), [AlL]+, in high-performance liquid chromatography (HPLC) was investigated using the C 18-bonded silica stationary phase. In this study, it was found that when the pH of the mobile phase was 5.5, a significant tailing of the alminium chelate was observed, whereas when the pH was 2, a marked improvement of the elution was attained. These results were interpreted in terms of a silanol masking effect: when an acidic mobile phase is employed, protonation of the silanol groups on the surface is maintained, while eliminating the strong interaction between the cationic chelate and the stationary phase. The addition of sodium chloride in the eluent can also be used to eliminate peak tailing of the cationic chelate. Based on these results, the underlying immobilization mechanism of the aluminium chelate on the C18-bonded silica thin layer is explained by an electrostatic interaction between the cationic aluminium chelate and deprotonated silanol groups on the solid surface. The example in this study should provide valuable information to improve the separation quality of cationic solutes in HPLC.
  Japan Society for Analytical Chemistry, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.51.833
  DOI ID：10.2116/bunsekikagaku.51.833, ISSN：0525-1931, SCOPUS ID：0036391532
• ランタン―アリザリンコンプレクソン―フッ素三元錯体の薄層クロマトグラフィー　　　　　　　　　　　　　　　
  荒坂真吾; 金子恵美子; 斎藤伸吾; 星 座; 赤塚邦彦
  分析化学, 巻：51, 号：9, 開始ページ：837, 終了ページ：839, 2002年
  The thin-layer chromatographic behavior of a lanthanum-alizarin complexone (ALC)-fluoride complex was investigated with particular interest being paid to the separation of the ternary complex from La-ALC. Although the spectral shift of the La-ALC chelate upon binding to the fluoride ion is widely used for the spectrophotometric determination of fluoride ion, there do not appear to be any reports on thin-layer chromatography (TLC) for the ternary complex (La-ALC-F). In this study, it was found that when developed with 60% ethanol on a C 18-bonded silica thin-layer plate, the ternary complex was easily eluted, forming a sharp band. Conversely, the La-ALC chelate was immobilized at the spotting position as a fleck. Furthermore, when a bluish-purple band was separated from a reddish-purple fleck, the ternary complex faded in a few minutes, whereas, when the band remained inside the fleck, no fading took place. These results are interpreted in terms of the dissociation of the labile complex caused by separation from the excess reagent. Consequently, the concentration of the La-ALC-F band across the center of the fleck provides a sensitive visualization of the fluoride ion down to 0.1 ppm.
  Japan Society for Analytical Chemistry, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.51.837
  DOI ID：10.2116/bunsekikagaku.51.837, ISSN：0525-1931, CiNii Articles ID：110002905144, SCOPUS ID：4143081672
• Entropy-controlled solvolytic dissociation kinetics of lanthanide(III) complexes with polyaminocarboxylates in aqueous solutions　　　　　　　　　　　　　　　
  S. Saito; H. Hoshino; T. Yotsuyanagi
  Inorganic Chemistry, 巻：40, 号：15, 開始ページ：3819, 終了ページ：3823, 2001年07月
  The factors involved in the formation of an inert complex in terms of solvolysis reaction have been studied for lanthanide (III)-acyclic polyaminocarboxylate complexes, as the basis for kinetically controlled selectivity used in analytical methodologies such as HPLC and HPCE. The rate constants for solvolysis and acid-assisted dissociation processes of the lanthanide complexes were determined in a batch system through metal-and ligand-exchange reactions. The reagents used were 8-amino-2-[(2-amino-5-methylphenoxy) methyl]-6-methoxyquinoline-N,N,N′,N′-tetraacetic acid (Quin2) and O,O′-bis(2-aminophenyl)ethylene glycol-N,N,N′,N′-tetraacetic acid (BAPTA) as octadentate ligands and trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CyDTA) as a hexadentate ligand. It has been found that the rate constants for solvolysis vary from 5.7 × 10-3 s-1 (La3+) to 1.7 × 10-6 s-1 (LU3+) depending on the ionic radii of Ln(III) ions for the Quin2 complexes, while no such monotonic dependence was observed for the BAPTA complexes. Among the parameters of activation, it is worth noting that there is a considerably large negative entropy of activation, of up to -250 J mol-1 K-1, and it is this which is responsible for the inertness of the Ln-polyaminocarboxylate complexes. Our data suggest that multiple ligation of the ligand in favor of the large coordination number of Ln(III) ions is of key importance for formation of the negative entropy of activation, in addition to the basicity of the ligand which also plays a significant role in the slow dissociation kinetics of the Ln(III) complexes.
  英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1021/ic001053k
  DOI ID：10.1021/ic001053k, ISSN：0020-1669, CiNii Articles ID：80012522035, PubMed ID：11442383, SCOPUS ID：0035898073
• Fluorescence determination of closed-shell rare earth metal ions by reversed-phase HPLC with precolumn derivatization using 8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N ',N '-tetraacetate　　　　　　　　　　　　　　　
  S Saito; WZ Xu; H Hoshino; T Yotsuyanagi
  BUNSEKI KAGAKU, 巻：50, 号：2, 開始ページ：113, 終了ページ：117, 2001年02月, ［査読有り］
  A sensitive and selective determination of closed-shell rare earth metal ions (La3+, Lu3+, and Y3+) has been demonstrated using a pre-column chelating reagent {8-amino-2-[(2-amino-5-methylphenoxy) methyl]-6-methoxvquinoline-N,N,N',N'-tetraacetatic acid (Quin2)}, in reversed-phase HPLC. Although the resolution is still unsatisfactory in this HPLC system, rare- earth metal chelates with this octadentate aromatic polyaminocarboxylate can be detected. This result suggests that these chelates are unexpectedly inert for the dissociation process during elution. The order of elution is quite different from that of the conventional ion-exchange mode separation. Middle-lanthanide ion-Quin2 chelates were most rapidly eluted, followed by the heavy ones; the light ones were the most strongly retained. Since ligand-centered fluorescence was observed for only La3+, Lu3+, Y3+, and Gd3+-Quin2 chelates, and other fare earth metal ion chelates were sufficiently quenched through a paramagnetic deactivation process, these four metal ions were selectively detected by fluorimetric detection (lambda (ex) = 335 nm, lambda (em) = 500 nm). The detection limits of La3+, Lu3+, and Y3+ were 2.2, 4.0, and 1.9 ppb, respectively, on a 3 sigma basis No interference from each 10-fold excess of other rare-earth metal ions was observed. The success of this HPLC system for rare-earth ions is derived from the combined properties of the Quin2 chelates in terms of inertness, chromatographic retention and fluorescence detection.
  JAPAN SOC ANALYTICAL CHEMISTRY, 日本語, 研究論文（学術雑誌）
  ISSN：0525-1931, Web of Science ID：WOS:000167071300003
• 8-アミノ-2-[(2-アミノ-5-メチルフェノキシ)メチル]-6-メトキシキノリン- N,N,N',N'-四酢酸を用いる閉殻希土類金属イオンのプレカラム誘導体化蛍光検出逆相分配高速液体クロマトグラフィー　　　　　　　　　　　　　　　
  齋藤 伸吾; 許 文哲; 星野 仁; 四ツ柳 隆夫
  分析化学, 巻：50, 号：2, 開始ページ：113, 終了ページ：117, 2001年
  A sensitive and selective determination of closed-shell rare earth metal ions (La3+, Lu3+, and Y3+) has been demonstrated using a pre-column chelating reagent {8-amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N′, N′-tetraacetatic acid (Quin2)}, in reversed-phase HPLC. Although the resolution is still unsatisfactory in this HPLC system, rare- earth metal chelates with this octadentate aromatic polyaminocarboxylate can be detected. This result suggests that these chelates are unexpectedly inert for the dissociation process during elution. The order of elution is quite different from that of the conventional ion-exchange mode separation. Middle-lanthanide ion-Quin2 chelates were most rapidly eluted, followed by the heavy ones
  the light ones were the most strongly retained. Since ligand-centered fluorescence was observed for only La3+, Lu3+, Y3+, and Gd3+-Quin2 chelates, and other rare earth metal ion chelates were sufficiently quenched through a paramagnetic deactivation process, these four metal ions were selectively detected by fluorimetric detection (λex= 335 nm, λem= 500 nm). The detection limits of La3+, Lu3+, and Y3+ were 2.2, 4.0, and 1.9 ppb, respectively, on a 3σ basis. No interference from each 10-fold excess of other rare-earth metal ions was observed. The success of this HPLC system for rare- earth ions is derived from the combined properties of the Quin2 chelates in terms of inertness, Chromatographic retention and fluorescence detection. © 2001 The Japan Society for Analytical Chemistry.
  Japan Society for Analytical Chemistry, 日本語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/bunsekikagaku.50.113
  DOI ID：10.2116/bunsekikagaku.50.113, ISSN：0525-1931, CiNii Articles ID：110002907394, SCOPUS ID：0035586503
• Dissociation rate constant estimation for the cerium(III)-O,O '-bis(2-aminoethyl)-ethyleneglycol-N,N,N ',N '-tetraacetate system by capillary electrophoresis　　　　　　　　　　　　　　　
  S Saito; H Hoshino; T Yotsuyanagi
  ANALYTICAL SCIENCES, 巻：16, 号：10, 開始ページ：1095, 終了ページ：1097, 2000年10月
  JAPAN SOC ANALYTICAL CHEMISTRY, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.2116/analsci.16.1095
  DOI ID：10.2116/analsci.16.1095, ISSN：0910-6340, CiNii Articles ID：10006675735, Web of Science ID：WOS:000090111000020
• Highly sensitive energy-transfer luminescence of the N,N '-bis(2-hydroxybenzyl)-ethylenediamine-N,N '-diacetatoterbium(III) complex in aqueous solution　　　　　　　　　　　　　　　
  S Saito; H Hoshino; T Yotsuyanagi
  BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 巻：73, 号：8, 開始ページ：1817, 終了ページ：1821, 2000年08月
  The luminescent properties of a terbium complex with a hexadentate ligand of N,N'-Bis(2-hydroxybenzyl)ethylenediamine-N,N'-diacetic acid (HBED) having a phenolate functional group as antenna moiety, have been investigated. The complex exhibits a high emission quantum yield (phi = 0.222, ex. 288 nm, em. 546 nm) with a long decay lifetime (tau H2O = 1.2 ms) in aqueous solution, which indicates energy-transfer type emission. The number of metal-bound water molecules in the complex was determined to be q = 1.8. The triplet energy level was estimated based on the data of its low-temperature phosphorescence. The calibration graph for Tb(III) was linear over the range from 3x10(-11) to 1x10(-8) mol dm(-3). A 3 sigma-blank detection limit of 3 ppt was achieved.
  CHEMICAL SOC JAPAN, 英語, 研究論文（学術雑誌）
  DOI：https://doi.org/10.1246/bcsj.73.1817
  DOI ID：10.1246/bcsj.73.1817, ISSN：0009-2673, eISSN：1348-0634, CiNii Articles ID：10009152815, Web of Science ID：WOS:000089388600014

• Front Cover (Hot Article): Holo/apo conversion two-dimensional urea PAGE for speciation of Fe3+-bound transferrin in serum　　　　　　　　　　　　　　　
  2024年02月
• Inside Cover (selected article): Americium(III)/Curium(III) Complete Separation and Sensitive Fluorescence Detection by Capillary and Gel Electrophoresis Using Emissive Hexadentate/Octadentate Polyaminocarboxylate Ligands　　　　　　　　　　　　　　　
  Shingo Saito
  2023年03月, ［筆頭著者, 責任著者］
  ISSN：0009-2673, eISSN：1348-0634
• Supplemental Cover: Unusually kinetically inert monocationic neptunyl complex with a fluorescein-modified 1,10-phenanthroline-2,9-dicarboxylate ligand: Specific separation and detection in gel electrophoresis
  Kazuhito Yamagata; Kazuki Marumo; Yuiko Tasaki-Handa; Tomoko Haraga; Shingo Saito
  2023年01月, ［責任著者］
  DOI：https://doi.org/10.1021/acs.inorgchem.2c02908
  DOI ID：10.1021/acs.inorgchem.2c02908, ISSN：0020-1669, eISSN：1520-510X
• Cover Feature: Single‐Round DNA Aptamer Selection by Combined Use of Capillary Electrophoresis and Next Generation Sequencing: An Aptaomics Approach for Identifying Unique Functional Protein‐Binding DNA Aptamers (Chem. Eur. J. 39/2021)
  Shingo Saito; Toshiki Sakamoto; Naoki Tanaka; Ryo Watanabe; Takuya Kamimura; Kazuki Ota; Kathryn R. Riley; Keitaro Yoshimoto; Yuiko Tasaki‐Handa; Masami Shibukawa
  巻：27, 号：39, 開始ページ：9963, 終了ページ：9963, 2021年07月12日, ［責任著者］
  DOI：https://doi.org/10.1002/chem.202102064
  DOI ID：10.1002/chem.202102064, ISSN：0947-6539, eISSN：1521-3765
• Front cover: Purification of anionic fluorescent probes through precise fraction collection with a two‐point detection system using multiple‐stacking preparative capillary transient isotachophoresis
  Tomoko Haraga; Hiroto Tsujimura; Saori Miyauchi; Takuya Kamimura; Masami Shibukawa; Shingo Saito
  巻：41, 号：13-14, 開始ページ：1152, 終了ページ：1159, 2020年07月, ［責任著者］
  DOI：https://doi.org/10.1002/elps.202070071
  DOI ID：10.1002/elps.202070071, ISSN：0173-0835, eISSN：1522-2683
• 超高温水におけるイオンの水和とイオン交換の選択性　　　　　　　　　　　　　　　
  原田誠; 岡田哲男; 中村圭介; 齋藤伸吾; 渋川雅美
  日本イオン交換研究発表会講演要旨集, 巻：34th, 2019年
  J-Global ID：201902275737674036
• 表面気泡変調液体クロマトグラフィーによるアセトニトリル水溶液/C₁₈シリカ界面における物質分離機構の解析-界面における物質分布におよぼす溶媒組成の影響の解明-　　　　　　　　　　　　　　　
  中村圭介; 生形竜介; 水野恒; 齋藤伸吾; 渋川雅美
  分析化学討論会講演要旨集, 巻：79th, 2019年
  J-Global ID：201902224568622010
• Front cover: A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis
  巻：142, 号：21, 開始ページ：3997, 終了ページ：3997, 2017年, ［責任著者］
  DOI：https://doi.org/10.1039/c7an90081c
  DOI ID：10.1039/c7an90081c, ISSN：0003-2654, eISSN：1364-5528
• 表面気泡変調液体クロマトグラフィーによる逆相分離系の溶質分離選択性におよぼす移動相組成の影響の解明　　　　　　　　　　　　　　　
  中村圭介; 生形竜介; 齋藤伸吾; 渋川雅美
  日本分析化学会年会講演要旨集, 巻：66th, 2017年
  J-Global ID：201702223555721156
• キャピラリー電気泳動法を用いる細菌細胞を認識するDNAアプタマーの高速選抜システム (特集 革新的ライフサイエンスの展望)　　　　　　　　　　　　　　　
  齋藤 伸吾
  化學工業, 巻：67, 号：7, 開始ページ：505, 終了ページ：512, 2016年07月
  小峰工業出版, 日本語
  ISSN：0451-2014, CiNii Articles ID：40020880448, CiNii Books ID：AN00037245
• Inside back cover: Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis
  巻：52, 号：3, 開始ページ：622, 終了ページ：622, 2016年, ［責任著者］
  DOI：https://doi.org/10.1039/c6cc90014c
  DOI ID：10.1039/c6cc90014c, ISSN：1359-7345, eISSN：1364-548X
• 表面気泡変調液体クロマトグラフィーによるアルキル結合型シリカを用いた逆相分離系における溶質保持に対する水/疎水界面およびアルキル結合層の寄与の評価　　　　　　　　　　　　　　　
  中村圭介; 齋藤伸吾; 渋川雅美
  分析化学討論会講演要旨集, 巻：76th, 2016年
  J-Global ID：201602229547081494
• 表面気泡変調液体クロマトグラフィーによる逆相分離系における溶質分離選択性に及ぼす充填剤表面化学構造の影響の解明　　　　　　　　　　　　　　　
  中村圭介; 齋藤伸吾; 渋川雅美
  日本分析化学会年会講演要旨集, 巻：65th, 2016年
  J-Global ID：201602249663885518
• 電気泳動法をプラットフォームとするタンパク質結合型金属イオンの分布測定法 (特集 次世代ライフサイエンスの展望)　　　　　　　　　　　　　　　
  齋藤 伸吾
  化學工業, 巻：66, 号：1, 開始ページ：21, 終了ページ：27, 2015年01月
  小峰工業出版, 日本語
  ISSN：0451-2014, CiNii Articles ID：40020308059, CiNii Books ID：AN00037245
• 表面気泡変調液体クロマトグラフィーによる疎水性表面における水の分離機能の解析　　　　　　　　　　　　　　　
  中村圭介; 齋藤伸吾; 渋川雅美
  分析化学討論会講演要旨集, 巻：75th, 2015年
  J-Global ID：201502219832148964
• 玄米中の各部位における放射性および非放射性セシウムの分析　　　　　　　　　　　　　　　
  加藤美佐; 岡田往子; 齋藤伸吾; 渋川雅美
  日本分析化学会年会講演要旨集, 巻：63rd, 開始ページ：47, 終了ページ：47, 2014年09月03日
  (公社)日本分析化学会, 日本語
  医中誌Web ID：2016161152, J-Global ID：201402204280764465
• 気液ハイブリッド固定相HPLCシステムの開発-分離機構の解明と分離能の評価-　　　　　　　　　　　　　　　
  中村圭介; 齋藤伸吾; 渋川雅美
  分析化学討論会講演要旨集, 巻：74th, 2014年
  J-Global ID：201402225589948568
• 気液ハイブリッド固定相分離システム構築のための気相形成方法の検討　　　　　　　　　　　　　　　
  中村圭介; 仲村大樹; 齋藤伸吾; 渋川雅美
  分析化学討論会講演要旨集, 巻：73rd, 2013年
  J-Global ID：201302229858922536
• フッ化炭素修飾シリカカラムを用いた高性能気液ハイブリッド分離システムの開発　　　　　　　　　　　　　　　
  中村圭介; 仲村大樹; 齋藤伸吾; 渋川雅美
  日本分析化学会年会講演要旨集, 巻：61st, 2012年
  J-Global ID：201202234079560217
• Front cover: Ultrasensitive CE for heavy metal ions using the variations in the chemical structures formed from new octadentate fluorescent probes and cationic polymers　　　　　　　　　　　　　　　
  巻：136, 号：13, 開始ページ：2657, 終了ページ：2657, 2011年, ［責任著者］
  DOI：https://doi.org/10.1039/c1an90042k
  DOI ID：10.1039/c1an90042k, ISSN：0003-2654, eISSN：1364-5528
• 剛性の異なる配位骨格を有する八座蛍光配位子錯体のPAGEにおける泳動挙動の調査
  榎本七基; 野村高弘; 吉本敬太郎; 佐藤誠; 齋藤伸吾; 前田瑞夫; 渋川雅美
  日本分析化学会年会講演要旨集, 巻：57th, 開始ページ：353, 2008年08月27日
  日本語
  J-Global ID：200902244874961904
• ポリアクリルアミドゲル電気泳動による金属イオンの高感度蛍光検出システムの開発
  齋藤伸吾; 榎本七基; 野村高弘; 吉本敬太郎; 佐藤誠; 前田瑞夫; 渋川雅美
  分析化学討論会講演要旨集, 巻：69th, 開始ページ：33, 2008年05月01日
  日本語
  J-Global ID：200902228170266377
• 水素結合性ランタノイド錯体を用いた新規核酸蛍光ラベリング法の構築
  厚見宙志; 吉本敬太郎; 斎藤伸吾; 大熊盛也; 前田瑞夫; 長崎幸夫
  生体機能関連化学シンポジウム講演要旨集, 巻：22nd, 開始ページ：516, 終了ページ：517, 2007年09月10日
  日本語
  J-Global ID：200902277266247846
• 水素結合性ランタノイド錯体の合成と核酸塩基認識能の評価
  厚見宙志; 吉本敬太郎; 齋藤伸吾; 大熊盛也; 前田瑞夫; 長崎幸夫
  日本分析化学会年会講演要旨集, 巻：56th, 開始ページ：378, 2007年09月05日
  日本語
  J-Global ID：200902261161655880
• キャピラリー電気泳動法における重金属イオンの直接蛍光検出:分子およびシステム設計
  竹内慎吾; 鈴木良治; 吉本敬太郎; 齋藤伸吾; 前田瑞夫; 青山政和
  日本分析化学会年会講演要旨集, 巻：55th, 開始ページ：294, 2006年09月06日
  日本語
  J-Global ID：200902247333460251

• 改訂6版 分析化学データブック　　　　　　　　　　　　　　　
  齋藤伸吾, ［共編者(共編著者)］
  丸善出版, 2021年11月
  総ページ数：260
  ISBN：4621306529, ASIN：4621306529, EAN：9784621306529
• ベーシックマスター 分析化学　　　　　　　　　　　　　　　
  齋藤伸吾(分担執筆), ［共著］
  オーム社, 2013年08月
  日本語, 総ページ数：450
  CiNii Books：http://ci.nii.ac.jp/ncid/BB13305748
  ISBN：4274214257, ASIN：4274214257, EAN：9784274214257, CiNii Books ID：BB13305748

• DNAアプタマー選抜を科学する　　　　　　　　　　　　　　　
  齋藤伸吾
  化学とマイクロ・ナノシステム学会 第48回研究会（CHEMINAS 48）, 2023年11月, ［招待有り］
• CE と機械学習による DNA アプタマー選抜　　　　　　　　　　　　　　　
  齋藤伸吾
  第42回キャピラリー電気泳動シンポジウム（SCE2022）, 2022年10月, ［招待有り］
• Single-round DNA aptamer selection by means of capillary electrophoresis combined with next generation sequencing: could unique functional aptamers be acquired by sequence data mining?　　　　　　　　　　　　　　　
  Shingo Saito
  The 2021 International Chemical Congress of Pacific Basin Societies (PacifiChem2021), 2021年12月, ［招待有り］
  英語, 口頭発表（招待・特別）
• キャピラリー電気泳動法によるDNAアプタマー-トロンビン三元複合体の結合サイト、平衡および速度パラメーターの測定　　　　　　　　　　　　　　　
  齋藤伸吾
  第72回日本電気泳動学会総会, 2021年07月, ［招待有り］
  口頭発表（招待・特別）
• CE分離と機械学習によるDNAアプタマー選抜　　　　　　　　　　　　　　　
  齋藤伸吾
  第28回クロマトグラフィーシンポジウム（CS28）, 2021年06月, ［招待有り］
  口頭発表（招待・特別）
• アクチノイドイオン分析への電気泳動法の適用　　　　　　　　　　　　　　　
  齋藤伸吾; 原賀智子
  第81回分析化学討論会, 2021年05月, ［招待有り］
  口頭発表（招待・特別）
• Multi-Dimensional PAGE for Isolation and Identification of Metalloproteins in Biological Samples　　　　　　　　　　　　　　　
  齋藤伸吾
  第43回日本分子生物学会年会 国際ワークショップ, 2020年12月, ［招待有り］
  英語, 口頭発表（招待・特別）
• ゲル電気泳動を用いるCu2+配位を介したHA超分子の化学量論組成の推定　　　　　　　　　　　　　　　
  中野純佳,斉藤拓巳,半田友衣子,齋藤伸吾
  日本腐植物質学会第36回講演会, 2020年11月
• CE による生体分子錯体の結合サイト、平衡および速度パラメーターの測定　　　　　　　　　　　　　　　
  齋藤伸吾
  第40回キャピラリー電気泳動シンポジウム（SCE2020）, 2020年11月, ［招待有り］
• ゲル電気泳動を用いるCu2+を介したHA超分子の由来が異なることによる超分子化挙動の比較　　　　　　　　　　　　　　　
  中野純佳; 松本篤正; 丸茂和樹; 斉藤拓巳; 半田友衣子; 齋藤伸吾
  第69回日本分析化学年会, 2020年09月
• シングルラウンドCE選抜を用いる巨大官能基修飾型新奇DNAアプタマーの創製　　　　　　　　　　　　　　　
  太田和希; 渡辺崚; 半田友衣子; 齋藤伸吾
  第69回日本分析化学年会, 2020年09月
• CE による機能性 DNA アプタマーの 1 ラウ ンド選抜　　　　　　　　　　　　　　　
  齋藤伸吾
  第 27 回クロマトグラフィーシンポジウム, 2020年06月, ［招待有り］
• ゲル電気泳動分離を用いる地下水および土壌フミン酸のCu2+およびTb3+による超分子化挙動の解析　　　　　　　　　　　　　　　
  中野純佳; 風見綸太郎; 丸茂和樹; 斉藤拓巳; 半田友衣子; 齋藤伸吾
  第80回分析化学討論会, 2020年05月
  その他
• 蛍光性フェナントロリンジカルボン酸誘導体を用いるネプツニルイオンの特異的な電気泳動分離検出法とその錯体化学特性　　　　　　　　　　　　　　　
  山縣和仁; 丸茂和樹; 大内和希; 渋川雅美; 半田友衣子; 原賀智子; 齋藤伸吾
  第80回分析化学討論会, 2020年05月
  その他
• 電気泳動法を基盤とした生体試料中微量金属イオンおよび金属タンパク質の分離検出法　　　　　　　　　　　　　　　
  齋藤伸吾
  第20回日本亜鉛栄養治療研究会, 2020年02月, ［招待有り］
• 1ラウンド電気泳動選抜と大規模配列解析に基づくタンパク質結合型DNAアプタマーの獲得　　　　　　　　　　　　　　　
  齋藤伸吾
  日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会, 2019年07月, ［招待有り］
• 新規分子と出会うための特異な電気泳動分離　　　　　　　　　　　　　　　
  齋藤伸吾
  日本分析化学会関東支部若手交流会, 2019年07月, ［招待有り］
• 固定化DNAを介して核酸アプタマーを修飾した金ナノ粒子によるトロンビンタンパク質の検出　　　　　　　　　　　　　　　
  吉村健; 矢野湧暉; 矢野雄暉; 小川敦司; 前田瑞夫; 朝日剛; 古性均; 齋藤伸吾; 吉本敬太郎; 座古保
  第79回分析化学討論会, 2019年05月, ［国内会議］
  日本語, 口頭発表（一般）
• 金属ー有機構造体[Fe3OF(H2O)2(C9H3O6)2] を固定相とする液体クロマトグラフィーにおける保持機構のスイッチング　　　　　　　　　　　　　　　
  吉川真帆; 半田友衣子; 齋藤伸吾; 渋川雅美
  第79回分析化学討論会, 2019年05月, ［国内会議］
  日本語, 口頭発表（一般）
• 表面気泡変調液体クロマトグラフィーによるアセトニトリル水溶液／C18シリカ界面における物質分離機構の解析 －界面における物質分布におよぼす溶媒組成の影響の解明－　　　　　　　　　　　　　　　
  中村圭介; 生形竜介; 水野恒; 齋藤伸吾; 渋川雅美
  第79回分析化学討論会, 2019年05月, ［国内会議］
  日本語, 口頭発表（一般）
• 立体構造の異なるランダムDNAサブライブラリーを用いるトロンビン結合型アプタマー選抜　　　　　　　　　　　　　　　
  坂本寿樹; 吉本敬太郎; 渋川雅美; 齋藤伸吾
  第79回分析化学討論会, 2019年05月, ［国内会議］
  日本語, 口頭発表（一般）
• アプタマーペアの電気泳動選抜と高性能多価アプタマーの開発　　　　　　　　　　　　　　　
  齋藤 伸吾
  日本化学会 第99春季年会, 2019年03月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• トロンビン結合型DNAアプタマーの1ラウンドCE選抜　　　　　　　　　　　　　　　
  齋藤 伸吾
  キャピラリー電気泳動シンポジウム（SCE2018）, 2018年12月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• 立体構造の異なるランダムDNAライブラリーを用いる機能別アプタマー選抜　　　　　　　　　　　　　　　
  坂本寿樹; 渋川雅美; 齋藤伸吾
  キャピラリー電気泳動シンポジウム（SCE2018）, 2018年12月, ［国内会議］
  日本語, ポスター発表
• 二次元ゲル電気泳動法を用いる金属-フミン酸超分子錯体の凝集機構の解明　　　　　　　　　　　　　　　
  丸茂和樹; 斉藤拓巳; 原賀智子; 渋川雅美; 齋藤伸吾
  第34回日本腐植物質学会講演会, 2018年11月, ［国内会議］
  日本語, ポスター発表
• アプタマー修飾金ナノ粒子を用いたトロンビン検出におけるアプタマー配列の検討　　　　　　　　　　　　　　　
  吉村健; 矢野湧暉; 矢野雄暉; 小川敦司; 前田瑞夫; 古性均; 齋藤伸吾; 吉本敬太郎; 座古保
  日本化学会中国四国支部大会, 2018年11月, ［国内会議］
  日本語
• キャピラリー電気泳動を用いるDNAアプタマー選抜と機能判別　　　　　　　　　　　　　　　
  齋藤 伸吾
  日本分析化学会中部支部岐阜地区講演会, 2018年11月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• メタロタンパク質の分離・同定に特化したホロ/アポ変換二次元PAGE：紅色非硫黄光合成細菌中の銅結合性タンパクの同定　　　　　　　　　　　　　　　
  比護大地; 石川順子; Anne Durand; Soufian Ouchane; 渋川雅美; 齋藤伸吾
  第6回メタロミクス研究フォーラム, 2018年11月, ［国内会議］
  日本語, ポスター発表
• DNAアプタマーペアの電気泳動的選抜と高機能多価アプタマーの合理的設計　　　　　　　　　　　　　　　
  齋藤伸吾; 吉本敬太郎
  日本分析化学会第67年会, 2018年09月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• DNAアプタマー選抜における分離・洗浄工程の重要性：微粒子導入型キャピラリー電気泳動の導入効果　　　　　　　　　　　　　　　
  吉本敬太郎; 齋藤伸吾
  日本分析化学会第67年会, 2018年09月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• メタロタンパク質マッピング法としてのMICS-BN-PAGEに関する最新トピック　　　　　　　　　　　　　　　
  齋藤 伸吾
  第69回日本電気泳動学会総会, 2018年08月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• 新しい分子系の発見のための電気泳動的アプローチ　　　　　　　　　　　　　　　
  齋藤 伸吾
  日本分析化学会第66年会, 2017年09月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• キャピラリー電気泳動法を用いる細菌および動物細胞を認識するDNAアプタマーの新規獲得法　　　　　　　　　　　　　　　
  齋藤 伸吾
  第67回日本電気泳動学会総会, 2016年08月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• 細胞のキャピラリー電気泳動とDNAアプタマー選抜　　　　　　　　　　　　　　　
  齋藤 伸吾
  第76回分析化学討論会, 2016年05月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• 発見を目指す電気泳動分離分析　　　　　　　　　　　　　　　
  齋藤 伸吾
  日本化学会北海道支部北見地区化学講演会, 2016年01月, ［招待有り］
  口頭発表（招待・特別）
• Fluorescence sensing of saccharides using boronic acid functionalized squarylium dyes and their applications to analytical methods　　　　　　　　　　　　　　　
  齋藤 伸吾
  The 7th East Asia Symposium on Functional Dyes and Advanced Materials (EAS7), 2015年09月, ［招待有り］, ［国際会議］
  英語, 口頭発表（招待・特別）
• 電気泳動分離場で機能する蛍光プローブ　　　　　　　　　　　　　　　
  齋藤 伸吾
  「分離機能とセンシング機能の化学」セミナー, 2015年03月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• キャピラリー電気泳動を用いる細菌に特異的なDNAアプタマーの高速選抜法の開発　　　　　　　　　　　　　　　
  齋藤 伸吾
  第２回 混相流に関する最先端科学技術シンポジウム, 2014年11月, ［招待有り］, ［国内会議］
  日本語, 口頭発表（招待・特別）
• キャピラリー電気泳動を用いる濃縮―分離―分取法の開発とその応用　　　　　　　　　　　　　　　
  齋藤 伸吾
  第65回日本電気泳動学会総会, 2014年10月, ［招待有り］, ［国内会議］
  口頭発表（招待・特別）
• タンパク質結合型金属イオンのポリアクリルアミドゲル電気泳動によるマッピング　　　　　　　　　　　　　　　
  齋藤 伸吾
  第65回日本電気泳動学会総会, 2014年10月, ［招待有り］
  口頭発表（招待・特別）
• Selection of DNA Aptamer for Bacterial Cell using Polymer-enhanced Capillary Transient Isotachophoresis　　　　　　　　　　　　　　　
  齋藤 伸吾
  1st Asian Symposium on Analytical Sciences, 2014年09月, ［招待有り］, ［国際会議］
  英語, 口頭発表（招待・特別）
• 超微量重金属イオンのCE蛍光検出のための試薬および分離システム設計　　　　　　　　　　　　　　　
  齋藤 伸吾
  第26回イオンクロマトグラフィー討論会, 2009年12月, ［招待有り］
  口頭発表（招待・特別）
• Superheated Water Ion-Exchange Chromatography: A New Approach to Alteration of Selectivity in Ion-Exchange Separation　　　　　　　　　　　　　　　
  2009年
• Polyacrylamide gel electrophoresis of trace metal ions bounded to proteins in gel fraction using novel fluorescent probes: Fluorescent detection of trace Fe(III) in transferrin　　　　　　　　　　　　　　　
  2009年
  ポスター発表
• 疎水性ナノ空間水が示す無機イオンの分離　　　　　　　　　　　　　　　
  第28回溶媒抽出討論会 講演要旨集, 2009年
• ポリエチレングリコール水溶液におけるCo(II)チオシアナト錯体生成反応 -高分子水溶液中の水の状態とその錯形成反応への影響-　　　　　　　　　　　　　　　
  日本分析化学会第59年会 講演要旨集, 2009年
  ポスター発表
• 水性ニ相抽出を用いた環境水中の低濃度亜鉛の簡易比色分析法の開発　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
  ポスター発表
• 八座発蛍光性配位子を用いる金属イオンのCE-LIF分離検出 -カチオン性ポリマーとのイオン会合による配位構造認識-　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカゲル充填剤内の疎水性微小空間における水の状態変化と分離に及ぼす効果　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
• PAGEをプラットフォームとするタンパク質結合型金属イオンの検出法の開発：トランスフェリン結合型Fe(III)の検出　　　　　　　　　　　　　　　
  東京コンファレンス2009 講演要旨集, 2009年
• 分離分析システムにおけるランタノイドイオンの高感度検出試薬の開発　　　　　　　　　　　　　　　
  齋藤伸吾
  「核燃料サイクルの物質利用」研究専門委員会, 2009年, ［招待有り］
  口頭発表（招待・特別）
• 配位子交換反応に基づく固相抽出による合成樹脂中のイミダゾールの分離濃縮　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 水性ニ相抽出法を用いた亜鉛の簡易比色分析法の開発　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 配位構造の異なる金属プローブ錯体のPAGEにおける分離検出特性の調査　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 剛性の高い八座非環状蛍光配位子を用いる金属イオンのキャピラリー電気泳動分離　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカ充填剤表面に形成する溶媒和液相とその溶質保持に及ぼす影響　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 純水を移動相とする逆相HPLCによる無機イオンの分離：疎水性ナノ空間水の分離機能　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカ充填剤表面に形成する溶媒和液相とその分離選択性　　　　　　　　　　　　　　　
  第70回分析化学討論会 講演要旨集, 2009年
• Superheated Water Ion-Exchange Chromatography: A New Approach to Alteration of Selectivity in Ion-Exchange Separation　　　　　　　　　　　　　　　
  2009年
• Polyacrylamide gel electrophoresis of trace metal ions bounded to proteins in gel fraction using novel fluorescent probes: Fluorescent detection of trace Fe(III) in transferrin　　　　　　　　　　　　　　　
  2009年
  ポスター発表
• 疎水性ナノ空間水が示す無機イオンの分離　　　　　　　　　　　　　　　
  第28回溶媒抽出討論会 講演要旨集, 2009年
• ポリエチレングリコール水溶液におけるCo(II)チオシアナト錯体生成反応 -高分子水溶液中の水の状態とその錯形成反応への影響-　　　　　　　　　　　　　　　
  日本分析化学会第59年会 講演要旨集, 2009年
  ポスター発表
• 水性ニ相抽出を用いた環境水中の低濃度亜鉛の簡易比色分析法の開発　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
  ポスター発表
• 八座発蛍光性配位子を用いる金属イオンのCE-LIF分離検出 -カチオン性ポリマーとのイオン会合による配位構造認識-　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカゲル充填剤内の疎水性微小空間における水の状態変化と分離に及ぼす効果　　　　　　　　　　　　　　　
  日本分析化学会第58年会 講演要旨集, 2009年
• PAGEをプラットフォームとするタンパク質結合型金属イオンの検出法の開発：トランスフェリン結合型Fe(III)の検出　　　　　　　　　　　　　　　
  東京コンファレンス2009 講演要旨集, 2009年
• 配位子交換反応に基づく固相抽出による合成樹脂中のイミダゾールの分離濃縮　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 水性ニ相抽出法を用いた亜鉛の簡易比色分析法の開発　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 配位構造の異なる金属プローブ錯体のPAGEにおける分離検出特性の調査　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 剛性の高い八座非環状蛍光配位子を用いる金属イオンのキャピラリー電気泳動分離　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカ充填剤表面に形成する溶媒和液相とその溶質保持に及ぼす影響　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• 純水を移動相とする逆相HPLCによる無機イオンの分離：疎水性ナノ空間水の分離機能　　　　　　　　　　　　　　　
  分離技術会 年会2009 講演要旨集, 2009年
  ポスター発表
• C18結合型シリカ充填剤表面に形成する溶媒和液相とその分離選択性　　　　　　　　　　　　　　　
  第70回分析化学討論会 講演要旨集, 2009年
• Dissociation Process Kinetics of Metal Chelates. -A Key Factor in the HPLC/HPCE Application-　　　　　　　　　　　　　　　
  Proceedings of International Trace Analysis Symposium '98 (ITAS '98), 1998年
• Dissociation Process Kinetics of Metal Chelates. -A Key Factor in the HPLC/HPCE Application-　　　　　　　　　　　　　　　
  Proceedings of International Trace Analysis Symposium '98 (ITAS '98), 1998年
• 二次元ゲル電気泳動法によるランタノイドおよびアクチノイド-フミン酸超分子錯体の凝集反応追跡　　　　　　　　　　　　　　　
  丸茂和樹; 斉藤拓巳; 原賀智子; 渋川雅美; 齋藤伸吾
  キャピラリー電気泳動シンポジウム（SCE2018）, ［国内会議］
  日本語, ポスター発表

• 2023年 - 現在
  分析化学I（埼玉大学）
• 2015年 - 現在
  分析化学II, 埼玉大学
• 2007年 - 現在
  分析化学演習, 埼玉大学
• 2007年 - 現在
  分析化学特論I, 埼玉大学
• 2007年 - 現在
  応用化学実験IV, 埼玉大学
• 2007年 - 現在
  ナノ化学特論II, 埼玉大学
• 2022年 - 2022年
  最先端生命科学研究特論（分担，集中講義）（千葉工業大学）
• 2007年 - 2017年
  応用化学実験II, 埼玉大学
• 2010年 - 2013年
  分析化学III, 埼玉大学
• 2007年 - 2012年
  確率・統計, 埼玉大学
• 2006年
  無機分析化学演習, 北見工業大学
• 2006年
  無機分析化学, 北見工業大学
• 2006年
  無機分析化学実験, 北見工業大学

• 日本電気泳動学会
• 日本イオン交換学会
• 日本腐植物質学会
• 日本分析化学会
• 日本化学会

• 天然有機物の非抽出・非破壊分析を可能にする固体EEM分光法の新規開拓　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 挑戦的研究(開拓), 2024年06月28日 - 2028年03月31日
  中屋 佑紀; 山村 寛; 藤嶽 暢英; 癸生川 陽子; 齋藤 伸吾, 北海道大学
  配分額（総額）：25870000, 配分額（直接経費）：19900000, 配分額（間接経費）：5970000
  課題番号：24K21328
• 新規の微生物モニタリング技術による未成熟な浄水プロセスの革新　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 基盤研究(A), 2024年04月01日 - 2028年03月31日
  佐藤 久; 山田 俊郎; 山村 寛; 黒田 恭平; 齋藤 伸吾; 中屋 佑紀, 北海道大学
  配分額（総額）：47060000, 配分額（直接経費）：36200000, 配分額（間接経費）：10860000
  課題番号：24H00325
• 分離化学とAI解析の融合による分子認識科学としてのDNAアプタオミクス　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2022年04月 - 2026年03月
  齋藤 伸吾, 埼玉大学, 研究代表者
  配分額（総額）：17290000, 配分額（直接経費）：13300000, 配分額（間接経費）：3990000
  課題番号：22H02104
• ポストPCR時代を見据えた宿主認識分子の検出に基づく病原体センサーの開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 2023年06月30日 - 2025年03月31日
  佐藤 久; 齋藤 伸吾; 中屋 佑紀, 北海道大学
  配分額（総額）：6500000, 配分額（直接経費）：5000000, 配分額（間接経費）：1500000
  課題番号：23K17767
• 電気泳動法を基盤とするアクチノイドに対する簡易・迅速スクリーニング分析法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 基盤研究(C), 2022年04月01日 - 2025年03月31日
  原賀 智子; 齋藤 伸吾, 国立研究開発法人日本原子力研究開発機構
  配分額（総額）：4290000, 配分額（直接経費）：3300000, 配分額（間接経費）：990000
  課題番号：22K05180
• 持続可能な小規模水道システム実現のための新しい水質センシング技術の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 基盤研究(A), 2021年04月05日 - 2025年03月31日
  佐藤 久; 山田 俊郎; 山村 寛; 黒田 恭平; 齋藤 伸吾, 北海道大学
  配分額（総額）：39780000, 配分額（直接経費）：30600000, 配分額（間接経費）：9180000
  Legionella pneumophilaと結合できるDNAアプタマーを選抜した。選抜は、ポリマー増強キャピラリー過渡的等速電気泳動法（PectI）を用いておこなった。PectIでは、微生物の細胞は移動の初期に過渡的等速電気泳動（tITP）の原理に基づき、狭い単一ピークとなる。tITP積層モードが完了した後プロセスはキャピラリーゾーン電気泳動（CZE）モードに移行し、PectIにおけるピークの分離を行う。分離された単一ピークは、泳動バッファー中に添加されたポリエチレンオキサイド（600,000）（PEO）の効果により、CZEモードの間ずっと維持される。このようにして特異的な結合親和性を有するDNAアプタマーを獲得できる。
  今年度は新規のLegionella pneumophila結合DNAアプタマーについて、PectI選抜法によるシングルラウンド選抜と、類似配列群に分けるクラスタリング法とディープラーニングを用いた定量的処理に基づく大規模NGS解析によって選抜することができた。この選抜システムで獲得できたDNAアプタマーの配列は、先行研究における、従来のCell-SELEXで選抜されたDNAアプタマーと比較して低い解離定数および速度定数を持っており、平衡論的、反応速度論的にLegionella pneumophilaと高い結合能を持ったDNAアプタマーを獲得できた。すなわち、先行研究で選抜されたDNAアプタマーよりもLegionella pneumophilaと特異的に結合し、安定した複合体を形成するアプタマーであると考えられ、我々が目標としているバイオセンサーの開発に適したDNAアプタマーを選抜できたと言える。
  課題番号：21H04568
• 革新的微生物モニタリング技術の開発と適用：アジアの水系感染症根絶への挑戦　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B)), 国際共同研究加速基金(国際共同研究強化(B)), 2020年10月27日 - 2024年03月31日
  佐藤 久; 齋藤 伸吾; 山村 寛; 丁 青, 北海道大学
  配分額（総額）：18720000, 配分額（直接経費）：14400000, 配分額（間接経費）：4320000
  本年度は、マイクロプレートリーダーを用いた簡易微生物測定法を開発した。測定手順は以下の通りである。市販の一般細菌用培地にサンプル水180μLを添加した。これを6ウェルマイクロプレートの各ウェルに添加した。マイクロプレートを37℃に設定したマイクロプレートリーダーにセットし、10分毎にウェルの波長630nmの吸光度を分析しながら24時間培養した。
  一般細菌数の異なる2つのサンプルをn=5でマイクロプレートリーダーにより吸光度の経時変化を測定した。一般細菌数77 CFU/mLのサンプルでは、大体10から20時間で対数増殖によるサンプルの濁りが開始した。これに対し、15 CFU/mLのサンプルでは、10から40時間で対数増殖が見られた。n=5のとき、サンプル量は合わせて900 μLであり、平均すると1ウェルに入っている細菌数は2から3個である。必ずしも均等にウェルに入っているとは限らないため、0または1個しか細菌が存在しないウェルが生じると考えられる。これにより、細菌濃度が低いサンプルでは誤差が大きくなると考えられる。そこで、0から5時間で対数増殖が見られたら10点、5から10時間で対数増殖が見られたら9点という様にデータをスコア化し、細菌数と比較した。1~100CFU/mLにおいて、スコアと細菌数との間に相関関係が見られた。公定法では陰性であったにもかかわらず、本法で陽性だったサンプルも存在した。これは寒天培地上ではコロニーを作らないが、液体培地中では増殖する細菌種がいることが理由と考えられる。すなわち本法では、公定法では見落とす飲料水の細菌汚染をも検出できることが示唆された。
  課題番号：20KK0090
• 気体及び超臨界流体を固定化した分離場を用いる高性能多機能型逆相HPLCの開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2019年04月 - 2022年03月
  渋川 雅美; 半田 友衣子; 齋藤 伸吾, 埼玉大学, 研究分担者
  配分額（総額）：18070000, 配分額（直接経費）：13900000, 配分額（間接経費）：4170000
  本研究は，異なる表面構造をもつ多孔質材料の細孔内に気体および超臨界流体を固定化し，これを分離場とする表面気泡変調液体クロマトグラフィーおよび超臨界流体固定化液体クロマトグラフィーシステムを構築して，疎水性ナノ空間における気体と超臨界流体の分離媒体としての機能を解明するとともに，従来にない分離技術を生み出すことを目的として行った。最初に疎水性物質表面に形成される界面液相の構造と疎水性物質表面の化学構造が分離選択性に与える影響の解明を行った。ついで，気液分離による前処理を必要としない水中の揮発性有機化合物の完全回収分析法と不活性ガスの高速分析法の開発を目的として研究を展開した。
  課題番号：19H02741
• 機能発現・判別を指向するDNAアプタマーの電気泳動選抜　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 基盤研究B, 2019年 - 2022年
  齋藤 伸吾, 研究代表者
  競争的資金
• 蛍光プローブを用いた簡易・迅速・低コストのオンサイト指標細菌一斉計測技術の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), 2019年06月28日 - 2021年03月31日
  佐藤 久; 羽深 昭; 齋藤 伸吾, 北海道大学
  配分額（総額）：6500000, 配分額（直接経費）：5000000, 配分額（間接経費）：1500000
  蛍光プローブを用いた簡易・迅速・低コストのオンサイト指標細菌一斉計測技術を開発した。大腸菌数40cfu/Lのサンプルは7時間程度で、4cfu/Lのサンプルでも13時間程度で検出できた。操作は未処理下水であってもいかなる前処理もなしに液体培地または寒天培地にサンプル水を供し、37℃に設定したマイクロプレートリーダーで蛍光強度を10分に一回自動で測定するのみと極めて簡便である。通常の下廃水処理水であれば3時間程度で大腸菌数を測定できるので、時間的にも空間的にも高頻度に大腸菌数を分析でき、処理効率の悪化を迅速に把握できる。測定培地は0.02mL程度であるので1サンプルあたりのコストは2円程度である。
  課題番号：19K21979
• 核酸アプタマー選抜系への粒子支援型キャピラリー電気泳動の導入と検証　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2018年04月 - 2021年03月
  吉本 敬太郎; 吉冨 徹; 齋藤 伸吾, 東京大学, 研究分担者
  配分額（総額）：17160000, 配分額（直接経費）：13200000, 配分額（間接経費）：3960000
  申請者は現在までに可溶性タンパク質であるトロンビンを標的とし、MACE を分離・洗浄工程として導入した核酸アプタマー選抜（MACE-SELEX）に成功している。本研究課題では、MACE-SELEX を用いて低分子量化合物、中分子量化合物（短鎖ペプチド）、非可溶性膜タンパク質（プロテオリポソーム）を標的分子としてMACE-SELEX を実行し、各標的分子に対する核酸アプタマーを獲得することを目的としている。本年度は、本研究目的を遂行する過程で、以下の５つの研究成果が得られた。
  【１】獲得した核酸アプタマーの高次構造解析（パラレル型Gカルテット構造の同定）を、従来法の放射性同位体標識を用いる手法を用いずに、分光学的手法のみで測定できる手法を考案した（Analyst, 143, 4022, 2018）。
  【２】標的分子と結合すると大きな蛍光強度変化を示す蛍光修飾DNAアプタマーのスクリーニング法を確立した（Anal.Sci.,35,113, 2019）。
  【３】蛍光性小分子に結合する RNA アプタマーの構造最適化に成功した。塩基変異を導入した三又構造が、RNAセンシングに有益な高次構造であることを明らかとした（RNA, 25, 590, 2019）。
  【４】MACE-SELEXで獲得した DNA アプタマーを用いる血液凝固制御/中和アプタマーシステムを構築した。獲得したアプタマーとtoeholdを導入した中和剤配列を併用し、血液凝固抑制を迅速に解除・制御するアプタマーシステムを構築した（Mol. Ther. Nucleic Acids, 16, 348, 2019）。
  【５】非天然型修飾核酸ライブラリーに対する MACE-SELEX に成功した。（Anal. Sci., 35, 585, 2019）。
  課題番号：18H02002
• ナノ気泡およびナノ超臨界流体を構成要素とする複合分離場の創製と高機能化　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2016年04月 - 2019年03月
  渋川 雅美; 齋藤 伸吾; 半田 友衣子, 埼玉大学, 研究分担者
  配分額（総額）：17940000, 配分額（直接経費）：13800000, 配分額（間接経費）：4140000
  水溶液中で気体が疎水性ナノ細孔に安定に固定化される現象に基づいて，気相，水／疎水性物質界面，および疎水性物質相が複合的に分離場として作用する表面気泡変調液体クロマトグラフィーを開発した。この技術は，アルキル結合型シリカだけでなく，他の疎水性多孔質材料を充填したカラムでも実施可能であることを示すとともに，これを用いて，水溶液中の化合物の分離分析に最も多用されている逆相液体クロマトグラフィーの分離機構を定量的に解明することに成功した。また，この技術に基づいて超臨界二酸化炭素を疎水性ナノ細孔に固定化し，これを固定相とする超臨界流体固定化液体クロマトグラフィーを開発した。
  課題番号：16H04161
• 高い細胞認識能を有するDNAアプタマーの高速な電気泳動選抜法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 基盤研究C, 2016年04月 - 2019年03月
  齋藤 伸吾, 研究代表者
  競争的資金
• 多次元電気泳動法を用いるアクチノイドイオンの腐植物質による保持挙動の解明　　　　　　　　　　　　　　　
  住友財団, 環境研究助成, 2017年 - 2018年
  齋藤 伸吾, 研究代表者
  競争的資金
• ナノバブルをプラットホームとするハイブリッド分離システムの開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2013年04月 - 2016年03月
  渋川 雅美; 齋藤 伸吾, 埼玉大学, 研究分担者
  配分額（総額）：18460000, 配分額（直接経費）：14200000, 配分額（間接経費）：4260000
  疎水性ナノ細孔が水溶液中でナノバブルを安定に保持できることを利用して，気相空間を含む複数の分離場を組み込んだハイブリッド固定相をもつHPLCを開発し，表面気泡変調液体クロマトグラフィー(SBMLC)と名づけた。細孔内への気相の導入により，固定相として機能する水／疎水性材料界面と疎水性材料自身とが分割される。ナノバブルはその体積を圧力によって変えることができるので，水／疎水性材料界面の面積を圧力によって制御することができ，分離選択性を変換することができる。さらにSBMLCにより，これまでに成し得なかった逆相系分離における疎水性材料自身と水／疎水性材料界面の寄与の定量的評価を初めて達成した。
  課題番号：25288062
• 金属タンパク質の分布測定を指向した多次元ポリアクリルアミドゲル電気泳動法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 基盤研究C, 2013年04月 - 2016年03月
  齋藤 伸吾, 研究代表者
  競争的資金
• ゲル電気泳動法を基盤とする環境中腐食物質-重金属錯体のスペシエーション　　　　　　　　　　　　　　　
  鉄鋼環境基金, 第36回環境助成研究（水質・一般）, 2015年 - 2015年
  齋藤 伸吾, 研究代表者
  競争的資金
• 金属イオン検出を新たな次元とする多次元PAGEによるメタロタンパク質分析法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 若手研究B, 2011年04月 - 2013年03月
  齋藤 伸吾, 研究代表者
  競争的資金
• アクチノイドイオン適合型キャピラリー電気泳動用蛍光プローブおよびプローブ錯体の精密分離検出技術の開発　　　　　　　　　　　　　　　
  日本原子力研究開発機構, 先行基礎工学研究, 2010年 - 2012年
  齋藤 伸吾, 研究代表者
  競争的資金
• 金属イオン-タンパク質マッピング電気泳動法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 若手研究B, 2009年04月 - 2011年03月
  齋藤 伸吾, 研究代表者
  競争的資金
• 高温高圧水を用いた反応選択的分離分析システムの開発と高温水化学反応解析への展開　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業 基盤研究(B), 基盤研究(B), 2008年 - 2011年
  渋川 雅美; 齋藤 伸吾, 埼玉大学, 研究分担者
  配分額（総額）：20020000, 配分額（直接経費）：15400000, 配分額（間接経費）：4620000
  高温高圧水を移動相とするクロマトグラフィー(SWC)を用いて高温高圧下での水溶液系におけるイオン交換分離機構の解明を行い,新しい分離選択性を生み出す温度制御型イオン交換SWCを開発した。また,疎水表面における水の構造変化とその分離機能を明らかにするとともに,反応解析のためのHPLC法を開発して水を分離媒体ならびに反応媒体として利用するシステム構築のための基礎研究を行った。さらに反応選択的高速分離分析システムとしての酸化還元化学種変換二次元HPLCを開発し,その有用性を実証した。
  課題番号：20350034
• 発蛍光性重金属錯体の動的三元錯平衡を用いるサブpptレベルキャピラリー電気泳動法　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費補助金 若手研究B, 2006年04月 - 2009年03月
  齋藤 伸吾, 研究代表者
  競争的資金
• ゲル電気泳動をプラットフォームとするメタローム解析技術の開発　　　　　　　　　　　　　　　
  旭硝子財団, 研究助成プログラム（第１分野・研究奨励）, 2008年 - 2009年
  齋藤 伸吾, 研究代表者
  競争的資金
• 簡易なメタローム解析技術の開発　　　　　　　　　　　　　　　
  長瀬科学技術振興財団, 研究助成（生化学）, 2007年 - 2007年
  齋藤 伸吾, 研究代表者
  競争的資金
• 血清中ｐｐｔレベル重金属イオンの一斉検出システムのキット化　　　　　　　　　　　　　　　
  JST, シーズ育成試験, 2005年 - 2005年
  齋藤 伸吾, 研究代表者
  競争的資金
• キャピラリー電気泳動流動反応器による生体分子コンプレックスの動的挙動解析法の開発　　　　　　　　　　　　　　　
  日本学術振興会, 科学研究費助成事業, 基盤研究(B), 2003年 - 2004年
  高橋 透; 星野 仁; 壹岐 伸彦; 齋藤 伸吾; 高橋 透, 東北大学
  配分額（総額）：13100000, 配分額（直接経費）：13100000
  CERの概念をマイクロチップキャピラリー電気泳動法に応用して,新しい手法を開発した.すなわち,金属錯体の解離反応速度論解析法の新しい手法としてMicrochip Capillary Electrophoretic Reactor (μCER)を開発することに成功した.ポリアミノカルボン酸の一種であるQuin-2を配位子として選択し,そのCe(III)錯体(CeL)をモデル化合物として解離反応速度論解析を行った.通常,CEの分離時間は数分〜数十分であるのに対して,マイクロチップCEは数秒〜数十秒と分離時間が短いため,CERでは観測できないようなより早い反応系の解離反応速度論解析がμCERを用いることで可能となる.また,μCERでは,分離チャンネル全長の時間分解イメージ(時間分解電気泳動図)が取得できるため,一回のマイクロチップCE実験を行うだけで速度解析データの取得が可能である.この様な,データ取得,解析のハイスループット化もμCERの特徴である.
  生体分子コンプレックスの動的挙動解析の新しい手法としてキャピラリー電気泳動反応器(Capillary Electrophoretic Reactor : CER)を利用することを試みた.モデル系として,抗ジゴキシン抗体(aDiglgG)-ジゴキシン(Dig)の抗原-抗体複合体系,および二本鎖DNA(dsDNAS)-エチジウムブロマイド(EtBr)のインターカレーション複合体を選び,CERを用いて各複合体の自己解離反応速度定数(k_d)の算出を試みた.dsDNA-EtBr系では,CE分離のタイムスケールに比べィンターカレーション複合体の解離反応が早いため,CERによるk_dは不可能であった.一方,aDiglgG-Dig系では,抗体(lgG)自体の電気泳動移動度が極めて小さい事に加え,またその分子量(約150,000)が抗原のDigのそれ(約800)に対して極めて大きく,CEにおけるaDiglgG-Dig複合体とフリーのaDiglgGとの相互分離を達成することができず,CERでの速度解析を行うことができなかった.
  課題番号：15350039

• アプタマーの選抜方法　　　　　　　　　　　　　　　
  齋藤伸吾; 廣瀬和生; 土田真帆; 渋川雅美; 佐藤誠, 特許権
  特許番号・登録番号：特許6781883
• アメリシウム及びキュリウム測定用蛍光プローブ、並びにアメリシウム及びキュリウムの分雛及び定量分析方法　　　　　　　　　　　　　　　
  原賀智子; 佐藤義行; 齋藤伸吾, 特許権
  特許番号・登録番号：特許6332669
• 核酸アプタマー　　　　　　　　　　　　　　　
  吉本敬太郎; 吉冨徹; 和久井幸二; 山口茜; 古庄均; 齋藤伸吾; 宮内さおり, 特許権
• タンパク質結合型核酸アプタマーの結合部位判別およびアプタマーペアの解離平衡定数とアロステリック効果測定法　　　　　　　　　　　　　　　
  齋藤伸吾; 宮内さおり; 吉本敬太郎, 特許権
• キャピラリー等速電気泳動法を用いる複数回大容量注入－濃縮－分離－分取精製法　　　　　　　　　　　　　　　
  齋藤伸吾; 辻村大翔; 原賀智子, 特許権
  特許番号・登録番号：特許6028997
• 蛍光性ウラン錯体を形成する化合物、その合成方法、ウラン測定用蛍光プローグ及びウランの測定方法　　　　　　　　　　　　　　　
  原賀智子; 齋藤伸吾; 佐藤義行, 特許権
  特許番号・登録番号：特許5834274
• COMPPOUND FOR FORMING FLUORESCENT URANIUM COMPLEX, METHOD FOR SYNTHESIZING THEREOF, FLUORESCENT PROBE FOR DETECTING URANIUM AND METHOD FOR ANALYZING URANIUM　　　　　　　　　　　　　　　
  Tomoko Haraga; Shingo Saito; Yoshiyuki Sato, 特許権
• 試料中の金属の測定方法　　　　　　　　　　　　　　　
  齋藤伸吾; 佐藤誠, 特許権
  特許番号・登録番号：特許5145511
• 金属測定用蛍光プローブ　　　　　　　　　　　　　　　
  齋藤伸吾; 鈴木良治; 引地篤; 佐藤誠, 特許権
  特許番号・登録番号：特許5055595
• 流れ分離分析法による金属測定に使用する試薬及び測定方法　　　　　　　　　　　　　　　
  齋藤伸吾; 穴田哲也; 星座, 特許権
  特許番号・登録番号：特許4571379

• Single-round DNA aptamer selection by combined use of CE and next generation sequencing　　　　　　　　　　　　　　　
  本人以外, Wiley Analytical Science MAGAZINE, 2021年06月, ［インターネットメディア］
• 合成DNA薬 治療効果20倍　　　　　　　　　　　　　　　
  本人以外, 日経産業新聞, 2018年10月18日, ［新聞・雑誌］
• 15種のアミノ酸，溶離液１つで分解 埼玉大が精製技術　　　　　　　　　　　　　　　
  本人以外, 日刊工業新聞, 2018年05月24日, ［新聞・雑誌］
• 堀場賞受賞　　　　　　　　　　　　　　　
  日本経済新聞（関西版）地方経済面, 2013年10月18日, ［新聞・雑誌］
• 堀場賞受賞　　　　　　　　　　　　　　　
  本人以外, 京都新聞, 2013年10月18日, ［新聞・雑誌］
• All in the sugar coating: Developing a CE method for detecting pathogenic bacteria　　　　　　　　　　　　　　　
  本人以外, 2012年03月19日, ［インターネットメディア］
• アルミニウムブランク値の化学的提言　　　　　　　　　　　　　　　
  本人以外, ぶんせき, 2006年, ［会誌・広報誌］