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SUZUKI Miho
| Material Science Division | Associate Professor |
| Department of Applied Chemistry |
Performance information
■ MISC- Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination
Miho Suzuki; Yuzuru Husimi; Hirokazu Komatsu; Koji Suzuki; Kenneth T. Douglas
Journal of the American Chemical Society, Volume：130, Number：17, First page：5720, Last page：5725, 30 Apr. 2008
Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease. © 2008 American Chemical Society.
English
DOI：https://doi.org/10.1021/ja710870e
DOI ID：10.1021/ja710870e, ISSN：0002-7863, CiNii Articles ID：80019516196, PubMed ID：18393422, SCOPUS ID：42649141969 - Quantum dot FRET Biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination
Miho Suzuki; Yuzuru Husimi; Hirokazu Komatsu; Koji Suzuki; Kenneth T. Douglas
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Volume：130, Number：17, First page：5720, Last page：5725, Apr. 2008
Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease.
AMER CHEMICAL SOC, English
DOI：https://doi.org/10.1021/ja710870e
DOI ID：10.1021/ja710870e, ISSN：0002-7863, CiNii Articles ID：80019516196, PubMed ID：18393422, Web of Science ID：WOS:000255360100038 - 生体内反応モニターバイオプローブの開発と病態診断チップ及びドラッグデリバリーナノデバイスへの応用
鈴木美穂
Volume：5 (18年度), First page：191, Last page：192, 2007 - 生体内反応モニターバイオプローブの開発と病態診断チップ及びドラッグデリバリーナノデバイスへの応用
鈴木美穂
総合研究機構研究プロジェクト研究成果報告書, Volume：5 (18年度), First page：191, Last page：192, 2007 - Creation of Highly Functional Biomolecules by Evolutionary Molecular Engineering (Saitama-Bio Project)
Md. Salimullah
Volume：7, First page：53, Last page：53, 2006
Japanese
ISSN：1347-4758, CiNii Articles ID：120001371310 - 高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）
西垣功一; 吉田昼也; 田山貴紘; 木下保則; 鈴木美穂; 内田秀和; 勝部昭明; 北村幸一郎; 高橋陽子; Md. Salimullah; 門脇知子; 山本健二
埼玉大学地域共同研究センター紀要, Volume：7, First page：53, Last page：53, 2006
Japanese
ISSN：1347-4758, CiNii Articles ID：120001371310 - 生体内、生体外共用生命現象可視化バイオプローブの開発と病態診断システムへの応用
鈴木美穂
Volume：16年度, 2005 - Generation of Functional Biomolecules By Evolutionary Molecular Engineering
MD. Salimullah
Volume：6, First page：83, Last page：83, 2005
Japanese
ISSN：1347-4758, CiNii Articles ID：120001371347 - 生体内、生体外共用生命現象可視化バイオプローブの開発と病態診断システムへの応用
鈴木美穂
総合研究機構研究プロジェクト研究成果報告書, Volume：16年度, 2005 - 高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）
西垣功一; 北村幸一郎; 高橋―本多陽子; 木下保則; 吉田昼也; MD. Salimullah; 鈴木美穂; 内田秀和; 勝部昭明
埼玉大学地域共同研究センター紀要, Volume：6, First page：83, Last page：83, 2005
Japanese
ISSN：1347-4758, CiNii Articles ID：120001371347 - A T-extended vector using a green fluorescent protein as an indicator
Yoichiro Ito; Miho Suzuki; Yuzuru Husimi
Gene, Volume：245, Number：1, First page：59, Last page：63, 07 Mar. 2000
T-extended vector (T-vector) is a useful tool for cloning PCR products directly. We exploited a novel T-vector using a green fluorescent protein (GFP) as an indicator based on insertional inactivation. The brightest GFP mutant was used for easy detection even under daylight. The 100 bp and 0.9 kb of PCR products were cloned, and the transformant colonies with inserts were adjudged by the fluorescent green-white screening. The GFP system was more sensitive to insertional inactivation than the β-galactosidase system at the conventional insertion sites. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
English
DOI：https://doi.org/10.1016/S0378-1119(00)00039-1
DOI ID：10.1016/S0378-1119(00)00039-1, ISSN：0378-1119, CiNii Articles ID：80011621544, PubMed ID：10713445, SCOPUS ID：0034615667 - A T-extended vector using a green fluorescent protein as an indicator
Y Ito; M Suzuki; Y Husimi
GENE, Volume：245, Number：1, First page：59, Last page：63, Mar. 2000
T-extended vector (T-vector) is a useful tool for cloning PCR products directly. We exploited a novel T-vector using a green fluorescent protein (GFP) as an indicator based on insertional inactivation. The brightest GFP mutant was used for easy detection even under daylight. The 100 bp and 0.9 kb of PCR products were cloned, and the transformant colonies with inserts were adjudged by the fluorescent green-white screening. The GFP system was more sensitive to insertional inactivation than the P-galactosidase system at the conventional insertion sites. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
ELSEVIER SCIENCE BV, English
DOI：https://doi.org/10.1016/S0378-1119(00)00039-1
DOI ID：10.1016/S0378-1119(00)00039-1, ISSN：0378-1119, CiNii Articles ID：80011621544, PubMed ID：10713445, Web of Science ID：WOS:000085869700007 - A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation
Yoichiro Ito; Miho Suzuki; Yuzuru Husimi
Biochemical and Biophysical Research Communications, Volume：264, Number：2, First page：556, Last page：560, 22 Oct. 1999
Green fluorescent protein (GFP) has been utilized as a powerful reporter of gene expression and protein localization in cells. We discovered a mutant carrying point mutation S208L from a UV-excitable GFP (F99S/ M153T/V163A). It had the enhanced fluorescence intensity. Introduction of the red-shifted mutations (F64L/S65T) to this mutant led to the GFP having the brightest mutants reported which were expressed in Escherichia coli and excited at 488 nm. The relative fluorescence intensities to that of wild-type GFP and GFPuv were increased about 120- and 10-fold, respectively. It was shown that the S208L mutation contributes to both a higher intrinsic brightness of GFP and a higher expression level in E. coli.
Academic Press Inc., English
DOI：https://doi.org/10.1006/bbrc.1999.1541
DOI ID：10.1006/bbrc.1999.1541, ISSN：0006-291X, PubMed ID：10529401, SCOPUS ID：0033595785 - A novel green fluorescence protein mutant with enhanced sensitivity at 488nm excitation for microanalysis
Volume：264, First page：556, Last page：560, 1999
DOI：https://doi.org/10.1006/bbrc.1999.1541
DOI ID：10.1006/bbrc.1999.1541 - Production of 1, 5-anhydroglucitol from 1, 5-anhydrofructose in erythroleukemia cell
SUZUKI M; KAMETANI S; UCHIDA K; AKANUMA H
Volume：240, Number：1, First page：23, Last page：29, 1996
DOI：https://doi.org/10.1111/j.1432-1033.1996.0023h.x
DOI ID：10.1111/j.1432-1033.1996.0023h.x, ISSN：0014-2956, CiNii Articles ID：80009151760, PubMed ID：8797831 - Production of 1, 5-anhydroglucitol from 1, 5-anhydrofructose in erythroleukemia cell
Eur.J.Biochem., Volume：240, Number：1, First page：23, Last page：29, 1996
DOI：https://doi.org/10.1111/j.1432-1033.1996.0023h.x
DOI ID：10.1111/j.1432-1033.1996.0023h.x, ISSN：0014-2956, CiNii Articles ID：80009151760, PubMed ID：8797831