Synthesis of Water-Soluble Glycopolymers Bearing Porphyrin by Means of Glycopolymer Assembly and Physical Properties of Glycopolymers Including Ability for Singlet Oxygen Production. Yuta Komano; Miho Suzuki; Takahiko Matsushita; Tetsuo Koyama; Yoshihiro Ishimaru; Ken Hatano; Koji Matsuoka
Biomacromolecules,
巻：26,
号：5,
開始ページ：3021,
終了ページ：3031, 2025年05月,
［国際誌］Although photodynamic therapy (PDT) is expected to offer advantages in terms of selectivity, increased efficacy, and reduced side effects, the low solubilities of photosensitizers in aqueous media are significant issues. In this study, porphyrin-based monomers were synthesized by acryloylation of known tetraphenylporphyrin [5-(4-aminophenyl)-10,15,20-(triphenyl)porphyrin] (TPP). Simple radical polymerization of the porphyrin monomer and known glycosyl monomers in the presence of acrylamide to avoid steric hindrance yielded the corresponding polymeric photosensitizers, water-soluble glycopolymers with porphyrin moieties. The introduction of a porphyrin core gave polymer fluorescence and reactive oxygen species generation properties, and the addition of d-lactose and N-acetyl-d-glucosamine, respectively, remarkably improved solubility in water. The glycopolymers had high optical absorption, emission, and excitation in the visible light range and a singlet oxygen (SO) generation characteristic of porphyrins in aqueous solution, suggesting incorporation of the TPP into the linear polymer. The glycopolymers are promising not only for PDT but also as anticancer agents.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acs.biomac.5c00121DOI ID：10.1021/acs.biomac.5c00121,
PubMed ID：40250833,
PubMed Central ID：PMC12077403 Development of molecular sensors based on fluorescent proteins for polarized macrophages identification. Udari Kalpana Bandaranayake; Hiroki Sato; Miho Suzuki
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
巻：40,
号：12,
開始ページ：2133,
終了ページ：2145, 2024年12月,
［国際誌］Macrophages are a type of white blood cells that play key roles in innate immune responses as a part of cellular immunity for host defence and tissue homeostasis. To perform diverse functions, macrophages show high plasticity by transforming to polarized states. They are mainly identified as unpolarized, pro-inflammatory and antiinflammatory states and termed as M0, M1 and M2 macrophages respectively. Discriminating polarized states is important due to strict implication with inflammatory conditions resulting in many diseases as chronic inflammation, neurodegeneration, and cancer etc. Many polarization protein markers have been identified and applied to investigate expression profiles through PCR and other techniques with antibodies. However, they are time and cost consuming and sometimes show insufficient performances. We focused on the mannose receptor (CD206) as representative marker of M2 macrophage recognising terminal mannose. We developed dose dependent mannosylated fluorescent proteins (FPs) by conjugations with mannose derivative for around 20 modifiable sites on FPs surfaces. Maximum modifications did not spoil various features of FPs. We found further sensitive and specific discriminations among M2, M1 and M0 macrophages after treating polarized macrophages with adequately conditioned FPs compared to already established approaches using anti CD206 antibody through flow cytometric analysis. These results might be derived from direct ligand utilizations and increased avidity due to multivalent bindings with abundantly modified multimeric FPs. Our strategy is simple but addresses disadvantages of preceding methods. Moreover, this strategy is applicable to detect other cell surface receptors as FPs can be modified with ligands or recognizable aptamer like molecules.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1007/s44211-024-00649-wDOI ID：10.1007/s44211-024-00649-w,
PubMed ID：39235677 Thermoanalytical and Kinetic Studies for the Thermal Stability of Emerging Pharmaceutical Pollutants Under Different Heating Rates. Christian Ebere Enyoh; Tochukwu Oluwatosin Maduka; Miho Suzuki; Senlin Lu; Qingyue Wang
Journal of xenobiotics,
巻：14,
号：4,
開始ページ：1784,
終了ページ：1806, 2024年11月,
［国際誌］Emerging pharmaceutical pollutants like ciprofloxacin (CIP) and ibuprofen (IBU) are frequently detected in aquatic environments, posing risks to ecosystems and human health. Since pollutants rarely exist alone in the environment, understanding the thermal stability and degradation kinetics of these compounds, especially in mixtures, is crucial for developing effective removal strategies. This study therefore investigates the thermal stability and degradation kinetics of CIP and IBU, under different heating rates. Thermogravimetric analysis (TGA) and differential thermal analysis (DTA) were employed to examine the thermal behavior of these compounds individually and in mixture (CIP + IBU) at heating rates of 10, 20, and 30 °C/min. The kinetics of thermal degradation were analyzed using both model-fitting (Coats-Redfern (CR)) and model-free (Kissinger-Akahira-Sunose (KAS), Flynn-Wall-Ozawa (FWO), and Friedman (FR)) methods. The results showed distinct degradation patterns, with CIP decomposing between 280 and 550 °C and IBU between 152 and 350 °C, while the mixture exhibited multistep decomposition in the 157-500 °C range. The CR model indicated first-order kinetics as a better fit for the degradation (except for IBU). Furthermore, CIP exhibits higher thermal stability and activation energy compared to IBU, with the KAS model yielding activation energies of 58.09 kJ/mol for CIP, 11.37 kJ/mol for IBU, and 41.09 kJ/mol for CIP + IBU mixture. The CIP + IBU mixture generally showed intermediate thermal properties, suggesting synergistic and antagonistic interactions between the compounds. Thermodynamic parameters (ΔH°, ΔG°, ΔS°) were calculated, revealing non-spontaneous, endothermic processes for all samples (except in the FWO method) with a decrease in molecular disorder and positive ΔG° values across all models and heating rates. The study found that higher heating rates led to less thermodynamically favorable conditions for degradation. These findings provide important information concerning the thermal behavior of these pharmaceutical pollutants, which can inform strategies for their removal from the environment and the development of more effective waste-treatment processes.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.3390/jox14040095DOI ID：10.3390/jox14040095,
PubMed ID：39584960,
PubMed Central ID：PMC11587104 Self-Assembly of Silole-Based Aggregation-Induced Emission Compounds with Green Fluorescent Protein under Physiological Conditions for Traceable and Versatile Drug Delivery. Miho Suzuki; Fumihiro Murakami; Md Shazadur Rahman; Yusuke Akui; Ken Hatano
ACS applied bio materials,
巻：7,
号：10,
開始ページ：6477,
終了ページ：6491, 2024年10月,
［国際誌］Biomacromolecules are viewed as promising drugs due to their specific functions in biological processes, biocompatibility, and pharmacological efficacy. Injective administration, chosen to avoid intestinal barriers, may in turn lead to immediate decay in the circulation system, unreliable targeting performance, or the induction of immune responses. For some biomacromolecules, chemically modified proteins have been developed for practical use. Various cargo or carrier systems are under development but have been delayed by technical difficulties. We present self-assembled nanocapsules with diameters ranging from 100 to 500 nm that can be deployed in physiological buffers to enclose various substances present in the buffers at the same time. Our amphiphilic nanocapsule, consisting of silole-core dendrimer products as the hydrophobic part and green fluorescent protein (GFP) derivatives as the hydrophilic part, connects and assembles spontaneously when mixed in solutions while engulfing dissolved or dispersed compounds together in a dose-dependent manner and shows unique optical characteristics because the dendrimer products exhibit aggregation-induced emission. Furthermore, the emission of the dendrimer causes considerable fluorescence resonance energy transfer (FRET) to GFP derivatives upon association. We could easily monitor assemblies by FRET states and particle sizes and have confirmed a stable presence in the buffer for at least a month. Further tracking of nanocapsules by fluorescence confirmed efficient uptake into some cancer cells. Nanocapsules based on GFP variants with or without a cell-surface-specific tag demonstrated that the tag improved the potential for specific targeted delivery. There were also indications that the nanocapsules became unstable after cellular uptake in the intracellular environment. We report here the simple preparation of traceable, stable, and biocompatible self-assembled nanocapsules as the basis for a versatile drug delivery system.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acsabm.4c00639DOI ID：10.1021/acsabm.4c00639,
PubMed ID：39256188 Study on Fast Liquefaction and Characterization of Produced Polyurethane Foam Materials from Moso Bamboo. Go Masuda; Satoshi Akuta; Weiqian Wang; Miho Suzuki; Yu Honda; Qingyue Wang
Materials (Basel, Switzerland),
巻：17,
号：15, 2024年07月,
［国際誌］Although bamboo is widely distributed in Japan, its applications are very limited due to its poor combustion efficiency for fuel. In recent years, the expansion of abandoned bamboo forests has become a social issue. In this research, the possibility of a liquefaction process with fast and efficient liquefaction conditions using moso bamboo as raw material was examined. Adding 20 wt% ethylene carbonates to the conventional polyethylene glycol/glycerol mixed solvent system, the liquefaction time was successfully shortened from 120 to 60 min. At the same time, the amount of sulfuric acid used as a catalyst was reduced from 3 wt% to 2 wt%. Furthermore, polyurethane foam was prepared from the liquefied product under these conditions, and its physical properties were evaluated. In addition, the filler effects of rice husk biochar and moso bamboo fine meals for the polyurethane foams were characterized by using scanning electron microscopy (SEM) and thermogravimetry and differential thermal analysis (TG-DTA), and the water absorption and physical density were measured. As a result, the water absorption rate of bamboo fine meal-added foam and the thermal stability of rice husk biochar-added foam were improved. These results suggested that moso bamboo meals were made more hydrophilic, and the carbon content of rice husk biochar was increased.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.3390/ma17153751DOI ID：10.3390/ma17153751,
PubMed ID：39124415,
PubMed Central ID：PMC11312812 Live Cell Monitoring of Separase Activity, a Key Enzymatic Reaction for Chromosome Segregation, with Chimeric FRET-Based Molecular Sensor upon Cell Cycle Progression. Md Shazadur Rahman; Yutaka Shindo; Kotaro Oka; Wataru Ikeda; Miho Suzuki
Biosensors,
巻：14,
号：4, 2024年04月,
［国際誌］Separase is a key cysteine protease in the separation of sister chromatids through the digestion of the cohesin ring that inhibits chromosome segregation as a trigger of the metaphase-anaphase transition in eukaryotes. Its activity is highly regulated by binding with securin and cyclinB-CDK1 complex. These bindings prevent the proteolytic activity of separase until the onset of anaphase. Chromosome missegregation and aneuploidy are frequently observed in malignancies. However, there are some difficulties in biochemical examinations due to the instability of separase in vitro and the fact that few spatiotemporal resolution approaches exist for monitoring live separase activity throughout mitotic processes. Here, we have developed FRET-based molecular sensors, including GFP variants, with separase-cleavable sequences as donors and covalently attached fluorescent dyes as acceptor molecules. These are applicable to conventional live cell imaging and flow cytometric analysis because of efficient live cell uptake. We investigated the performance of equivalent molecular sensors, either localized or not localized inside the nucleus under cell cycle control, using flow cytometry. Synchronized cell cycle progression rendered significant separase activity detections in both molecular sensors. We obtained consistent outcomes with localized molecular sensor introduction and cell cycle control by fluorescent microscopic observations. We thus established live cell separase activity monitoring systems that can be used specifically or statistically, which could lead to the elucidation of separase properties in detail.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.3390/bios14040192DOI ID：10.3390/bios14040192,
PubMed ID：38667185,
PubMed Central ID：PMC11048197 Preparation of a Water-Soluble Glycopolymer Bearing Porphyrin Skeletons and Its Biological Properties. Yoshihiro Ishimaru; Tomohide Moteki; Miho Suzuki; Tetsuo Koyama; Takahiko Matsushita; Ken Hatano; Koji Matsuoka
ACS omega,
巻：8,
号：40,
開始ページ：37451,
終了ページ：37460, 2023年10月,
［国際誌］A known tetraphenyl porphyrin (TPP) having an amino functional group [5-(4-aminophenyl)-10,15,20-(triphenyl)porphyrin] was converted into the corresponding monomer by means of condensation with acryloyl chloride. Simple radical polymerization of the porphyrin monomer and a glycosyl monomer in the presence of acrylamide as a regulator monomer in order to avoid steric interference gave a water-soluble glycopolymer bearing porphyrin moieties. Spectroscopic analyses suggested incorporation of porphyrin moieties in the glycopolymer. The physical properties of the water-soluble glycopolymer bearing porphyrin moieties were examined in aqueous media, and the results also indicated the incorporation of TPP moieties in the polymer. Uptake of the polymer into HeLa cells was observed, and the cytotoxicity of the polymer was confirmed by microscopic analyses. The glycopolymer bearing porphyrin moieties is promising not only for photodynamic therapy but also as an anti-cancer reagent.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/acsomega.3c05581DOI ID：10.1021/acsomega.3c05581,
PubMed ID：37841131,
PubMed Central ID：PMC10568584 Development of Ratiometric Fluorescence Sensing Molecule for Caspase-14, a Key Enzyme of Epidermis Metabolism Miho Suzuki; Koki Taguma; Udari Kalpana Bandaranayake; Wataru Ikeda
Journal of Biomedical Research & Environmental Sciences,
巻：4,
号：4,
開始ページ：793,
終了ページ：800, 2023年04月
Skin is the vastest organ harboring principle functions to protect from external stimuli and internal moisture desorption. The loss of functions might bring about diseases and senescence and well maintained metabolisms are recognized as crucial points to prevent those situations. But due to complexities of skin tissues, there would be some difficulties in progress of elucidation for skin metabolisms in detail. Various differentiated tissues layered in order can form dermis and epidermis to exfoliate as scurf in the top layer finally. Caspase-14 categorized in cysteine-aspartic acid protease (Caspase) family that has been identified as key enzymes to promote programmed cell deaths by sequential activations is known to be specifically expressed in the upper tissues and involved in the differentiation, but not verified to have relationship with final exfoliation processes at the time of cell death. We then developed Fluorescence Resonance Energy Transfer (FRET) based sensing molecules to monitor Caspase-14 activity quantitatively. We utilized Green Fluorescent Protein (GFP) and organic fluorescent dyes because FRET phenomena could be achieved under condition with regulated interactions between at least two fluorescent molecules. The elaborately designed fluorescent complexes could render quantifiable sensing over fluorescent background but be simply introduced into cells via endocytic mechanisms to detect intracellular enzymatic activities alive. In this study, we demonstrated Caspase-14 activation during apoptosis induction in cultured normal epidermal cells that suggested possibilities of Caspase-14 contribution to programmed cell deaths.
SciRes Literature LLC, 研究論文（学術雑誌）
DOI：https://doi.org/10.37871/jbres1737DOI ID：10.37871/jbres1737,
eISSN：2766-2276 Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades. Miho Suzuki; Yutaka Shindo; Ryu Yamanaka; Kotaro Oka
Scientific reports,
巻：12,
号：1,
開始ページ：21160,
終了ページ：21160, 2022年12月,
［国際誌］Understanding cellular signaling flow is required to comprehend living organisms. Various live cell imaging tools have been developed but challenges remain due to complex cross-talk between pathways and response heterogeneities among cells. We have focused on multiplex live cell imaging for statistical analysis to address the difficulties and developed simple multiple fluorescence imaging system to quantify cell signaling at single-cell resolution using Förster Resonance Energy Transfer (FRET)-based chimeric molecular sensors comprised of fluorescent proteins and dyes. The dye-fluorescent protein conjugate is robust for a wide selection of combinations, facilitating rearrangement for coordinating emission profile of molecular sensors to adjust for visualization conditions, target phenomena, and simultaneous use. As the molecular sensor could exhibit highly sensitive in detection for protease activity, we customized molecular sensor of caspase-9 and combine the established sensor for caspase-3 to validate the system by observation of caspase-9 and -3 dynamics simultaneously, key signaling flow of apoptosis. We found cumulative caspase-9 activity rather than reaction rate inversely regulated caspase-3 execution times for apoptotic cell death. Imaging-derived statistics were thus applied to discern the dominating aspects of apoptotic signaling unavailable by common live cell imaging and proteomics protein analysis. Adopted to various visualization targets, the technique can discriminate between rivalling explanations and should help unravel other protease involved signaling pathways.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1038/s41598-022-25286-zDOI ID：10.1038/s41598-022-25286-z,
PubMed ID：36476686,
PubMed Central ID：PMC9729311 Evaluation of Caspase-3 Activity During Apoptosis with Fluorescence Lifetime-Based Cytometry Measurements and Phasor Analyses. Kapil Nichani; Jianzhi Li; Miho Suzuki; Jessica P Houston
Cytometry. Part A : the journal of the International Society for Analytical Cytology,
巻：97,
号：12,
開始ページ：1265,
終了ページ：1275, 2020年12月,
［国際誌］Caspase-3 is a well-described protease with many roles that impact the fate of a cell. During apoptosis, caspase-3 acts as an executioner caspase with important proteolytic functions that lead to the final stages of programmed cell death. Owing to this key role, caspase-3 is exploited intracellularly as a target of control of apoptosis for therapeutic outcomes. Yet the activation of caspase-3 during apoptosis is challenged by other roles and functions (e.g., paracrine signaling). This brief report presents a way to track caspase-3 levels using a flow cytometer that measures excited state fluorescence lifetimes and a signal processing approach that leads to a graphical phasor-based interpretation. An established Förster resonance energy transfer (FRET) bioprobe was used for this test; the connected donor and acceptor fluorophore is cleavable by caspase-3 during apoptosis induction. With the cell-by-cell decay kinetic data and phasor analyses we generate a caspase activation trajectory, which is used to interpret activation throughout apoptosis. When lifetime-based cytometry is combined with a FRET bioprobe and phasor analyses, enzyme activation can be simplified and quantified with phase and modulation data. We envision extrapolating this approach to high content screening, and reinforce the power of phasor approaches with cytometric data. Analyses such as these can be used to cluster cells by their phase and modulation "lifetime fingerprint" when the intracellular fluorescent probe is utilized as a sensor of enzyme activity. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/cyto.a.24207DOI ID：10.1002/cyto.a.24207,
PubMed ID：32790129,
PubMed Central ID：PMC7738394 Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy. Takayuki Umakoshi; Hikari Udaka; Takayuki Uchihashi; Toshio Ando; Miho Suzuki; Takeshi Fukuda
Colloids and surfaces. B, Biointerfaces,
巻：167,
開始ページ：267,
終了ページ：274, 2018年07月,
［国際誌］Conjugates of semiconductor quantum dots (QDs) and antibodies have emerged as a promising bioprobes due to their great combination of QD's efficient fluorescence and the high specificity of antigen-antibody reactions. For further developments in this field, it is essential to understand the molecular conformation of the QD-antibody conjugates at the single-molecule scale. Here, we report on the direct imaging of QD-antibody conjugates at the single-molecule scale by using high-speed atomic force microscopy (HS-AFM). Owing to the high spatiotemporal resolution of HS-AFM, we observed the dynamic splitting of individual antibodies during the conjugation process. QD-antibody conjugates were also clearly visualized at the single-molecule scale details. Several important features were even discovered through dynamic observation of the QD-antibody conjugates. We observed an intermediate state of conjugation, where the antibodies attached and detached to QDs repeatedly. We also revealed that the attached antibodies were not steady but drastically fluctuated in their recognition areas due to the Brownian motion. We also demonstrated that HS-AFM observation is useful for the quantitative analysis of fabricated conjugates.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.colsurfb.2018.04.015DOI ID：10.1016/j.colsurfb.2018.04.015,
PubMed ID：29677598 Quantum dot-linked immunosorbent assay (QLISA) using orientation-directed antibodies. Miho Suzuki; Hikari Udaka; Takeshi Fukuda
Journal of pharmaceutical and biomedical analysis,
巻：143,
開始ページ：110,
終了ページ：115, 2017年09月,
［国際誌］An approach similar to the enzyme-linked immunosorbent assay (ELISA), with the advantage of saving time and effort but exhibiting high performance, was developed using orientation-directed half-part antibodies immobilized on CdSe/ZnS quantum dots. ELISA is a widely accepted assay used to detect the presence of a target substance. However, it takes time to quantify the target with specificity and sensitivity owing to signal amplification. In this study, CdSe/ZnS quantum dots are introduced as bright and photobleaching-tolerant fluorescent materials. Since hydrophilic surface coating of quantum dots rendered biocompatibility and functional groups for chemical reactions, the quantum dots were modified with half-sized antibodies after partial reduction. The half-sized antibody could be bound to a quantum dot through a unique thiol site to properly display the recognition domain for the core process of ELISA, which is an antigen-antibody interaction. The reducing conditions were investigated to generate efficient conjugates of quantum dots and half-sized antibodies. This was applied to IL-6 detection, as the quantification of IL-6 is significant owing to its close relationships with various biomedical phenomena that cause different diseases. An ELISA-like assay with CdSe/ZnS quantum dot institution (QLISA; Quantum dot-linked immunosorbent assay) was developed to detect 0.05ng/mL IL-6, which makes it sufficiently sensitive as an immunosorbent assay.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jpba.2017.05.014DOI ID：10.1016/j.jpba.2017.05.014,
PubMed ID：28582666 Size distribution and sources of 37 toxic species of particulate polycyclic aromatic hydrocarbons during summer and winter in Baoshan suburban area of Shanghai, China. Qingyue Wang; Keisuke Kobayashi; Weiqian Wang; Jie Ruan; Daisuke Nakajima; Mayuko Yagishita; Senlin Lu; Wenchao Zhang; Miho Suzuki; Tomoya Saitou; Kazuhiko Sekiguchi; Kenshi Sankoda; Yuji Takao; Masaki Nagae; Masanori Terasaki
The Science of the total environment,
巻：566-567,
開始ページ：1519,
終了ページ：1534, 2016年10月,
［国際誌］The objectives of this study were to assess the size-segregated distribution and sources of 37 different species of particulate polycyclic aromatic hydrocarbons (PAHs) in a suburban area of Shanghai metropolitan City, China. The ambient particulate sampling was carried out on the rooftop of a five-stories building in Baoshan campus of Shanghai University. An Andersen high-volume air sampler was employed to collect ambient size-segregated particulate matter during summer of August to September and winter of November to December 2015. The high toxic PAHs were determined by a gas chromatography mass spectrometry. The concentrations of total PAHs in suspended particulate matter (SPM) and PM1.1 (suspended particulate matter below 1.1μm in diameter) in the suburban area of Shanghai were 4.58-14.5ng/m(3) and 1.82-8.56ng/m(3), respectively in summer, and 43.6-160ng/m(3) and 23.2-121ng/m(3), separately in winter. 1,8-Naphthalic anhydride (1,8-NA) showed the highest concentration among 37 different species of PAHs in the suburban area of Shanghai. The concentrations of high molecular PAHs (e.g. 5-6 ring PAHs) followed a nearly unimodal size distribution with the highest peaks in PM1.1. The diagnostic ration qualitatively indicated that PAHs in SPM of Shanghai were mainly derived from motor-vehicle or petroleum combustion in summer and from coal and biomass combustion in winter. According to the calculated toxicity equivalency factors based on the methods of Nisbet and Lagoy and the potency equivalency factors (PEF) recommended by U.S. EPA, the highest contributors in the total carcinogenicity of the PAHs in SPM and PM1.1 were dibenzo[a,h]pyrene (46.2% and 45.0% in summer), benzo[a]pyrene (44.4% and 43.8% in winter) and benz[j]aceanthrylene (80.2% and 83.1% in summer and 83.1% and 84.0% in winter), respectively. Therefore, benzo[a]pyrene seemed to be a lower contributor than other carcinogenic PAHs.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.scitotenv.2016.06.039DOI ID：10.1016/j.scitotenv.2016.06.039,
PubMed ID：27320739 Precise Fractionation of CdSe/ZnS Quantum Dot-Organic-Dye Conjugates Using a Gel Filtration Column. Takeshi Fukuda; Tomokazu Kurabayashi; Nayuta Funaki; Hikari Udaka; Miho Suzuki
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
巻：32,
号：5,
開始ページ：529,
終了ページ：34, 2016年,
［国際誌］Conjugates of semiconductor quantum dots (QDs) and organic dyes have been receiving attention as fluorescence biological sensing materials. In designing such sensors, a most important parameter is the number of organic-dye molecules that conjugate to a QD. If a precise separation method was developed, it might be possible to control conjugation without knowing the exact number of conjugated dye molecules per QD. In this study, the difference in linear velocities in a gel filtration column between CdSe/ZnS QDs and 5-(and 6)-carboxynaphthofluorescein succinimidyl ester is used. The velocities differ because the hydrophilicity of CdSe/ZnS QDs is much higher than that of the organic dye; hence, CdSe/ZnS-organic-dye conjugation can be controlled by changing the fraction number. Furthermore, the concentrations of CdSe/ZnS QDs and organic dye in fractionated solutions can be determined by measuring fluorescence spectra, and we demonstrate a fluorescence-type pH sensor based on the conjugate, which has a pH-sensitivity range from 7.5 - 9.5.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.2116/analsci.32.529DOI ID：10.2116/analsci.32.529,
PubMed ID：27169652 A high-throughput direct fluorescence resonance energy transfer-based assay for analyzing apoptotic proteases using flow cytometry and fluorescence lifetime measurements. Miho Suzuki; Ichiro Sakata; Takafumi Sakai; Hiroaki Tomioka; Koichi Nishigaki; Marc Tramier; Maïté Coppey-Moisan
Analytical biochemistry,
巻：491,
開始ページ：10,
終了ページ：7, 2015年12月,
［国際誌］Cytometry is a versatile and powerful method applicable to different fields, particularly pharmacology and biomedical studies. Based on the data obtained, cytometric studies are classified into high-throughput (HTP) or high-content screening (HCS) groups. However, assays combining the advantages of both are required to facilitate research. In this study, we developed a high-throughput system to profile cellular populations in terms of time- or dose-dependent responses to apoptotic stimulations because apoptotic inducers are potent anticancer drugs. We previously established assay systems involving protease to monitor live cells for apoptosis using tunable fluorescence resonance energy transfer (FRET)-based bioprobes. These assays can be used for microscopic analyses or fluorescence-activated cell sorting. In this study, we developed FRET-based bioprobes to detect the activity of the apoptotic markers caspase-3 and caspase-9 via changes in bioprobe fluorescence lifetimes using a flow cytometer for direct estimation of FRET efficiencies. Different patterns of changes in the fluorescence lifetimes of these markers during apoptosis were observed, indicating a relationship between discrete steps in the apoptosis process. The findings demonstrate the feasibility of evaluating collective cellular dynamics during apoptosis.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.ab.2015.08.022DOI ID：10.1016/j.ab.2015.08.022,
PubMed ID：26334608 Chemical Reaction in Microdroplets with Different Sizes Containing CdSe/ZnS Quantum Dot and Organic Dye. Takeshi Fukuda; Tomokazu Kurabayashi; Hikari Udaka; Nayuta Funaki; Miho Suzuki; Dong Hyun Yoon; Asahi Nakahara; Tetsushi Sekiguchi; Shuichi Shoji
IEICE Transactions on Electronics,
巻：98-C,
号：2,
開始ページ：123,
終了ページ：126, 2015年
研究論文（学術雑誌）
DOI：https://doi.org/10.1587/transele.E98.C.123DOI ID：10.1587/transele.E98.C.123,
DBLP ID：journals/ieicet/FukudaKUFSYNSS15 Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interactions. Naoto Nemoto; Takayuki Fukushima; Shigefumi Kumachi; Miho Suzuki; Koichi Nishigaki; Tai Kubo
Analytical chemistry,
巻：86,
号：17,
開始ページ：8535,
終了ページ：40, 2014年09月,
［国際誌］Immobilization of a protein in a functionally active form and correct orientation for high-throughput analysis is crucial for surface-based protein-molecular interaction studies and should aid progress in associated nanotechnologies. Here, we present a general method for controlled and oriented immobilization of proteins by a puromycin-linker for cDNA display technology. The utility and potential of this method was demonstrated by examining the interaction between the B domain of protein A and immunoglobulin G (IgG) by surface plasmon resonance. This study revealed that the mRNA fragment of the mRNA-protein fusion (i.e., mRNA display) interferes with the interaction between the protein (B domain) and its target molecule (IgG). This results in a reduction of the apparent affinity by ~10-fold. This method is expected to find wide appeal in the fields of surface-based studies of protein-protein interactions, drug screening, and single molecule analysis that require only a small amount of protein sample.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/ac501601gDOI ID：10.1021/ac501601g,
PubMed ID：25079196 Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies. Harshita Sharma; Yasunori Kinoshita; Seiichi Fujiu; Shota Nomura; Mizuho Sawada; Shamim Ahmed; Masaki Shibuya; Kosaku Shirai; Syota Takamatsu; Tsuyoshi Watanabe; Hitoshi Yamazaki; Ryohei Kamiyama; Tetsuya Kobayashi; Hidenao Arai; Miho Suzuki; Naoto Nemoto; Ki Ando; Hidekazu Uchida; Koichiro Kitamura; Osamu Takei; Koichi Nishigaki
BMC biotechnology,
巻：14,
開始ページ：78,
終了ページ：78, 2014年08月,
［国際誌］BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1186/1472-6750-14-78DOI ID：10.1186/1472-6750-14-78,
PubMed ID：25141858,
PubMed Central ID：PMC4153446 Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method. Harshita Sharma; Fumihito Ohtani; Parmila Kumari; Deepti Diwan; Naoko Ohara; Tetsuya Kobayashi; Miho Suzuki; Naoto Nemoto; Yoshibumi Matsushima; Koichi Nishigaki
Biophysics (Nagoya-shi, Japan),
巻：10,
開始ページ：55,
終了ページ：62, 2014年,
［国内誌］Familial clustering without any prerequisite knowledge becomes often necessary in Behavioral Science, and forensic studies in case of great disasters like Tsunami and earthquake requiring body-identification without any usable information. However, there has been no well-established method for this purpose although conventional ones such as short tandem repeats (STR) and single nucleotide polymorphism (SNP), which might be applied with toil and moil to some extent. In this situation, we could find that the universal genome distance-measuring method genome profiling (GP), which is made up of three elemental techniques; random PCR, micro-temperature gradient gel electrophoresis (μTGGE), and computer processing for normalization, can do this purpose with ease when applied to mouse families. We also confirmed that the sequencing approach based on the ccgf (commonly conserved genetic fragment appearing in the genome profile) was not completely discriminative in this case. This is the first demonstration that the familial clustering can be attained without a priori sequence information to the level of discriminating strains and sibling relationships. This method can complement the conventional approaches in preliminary familial clustering.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.2142/biophysics.10.55DOI ID：10.2142/biophysics.10.55,
PubMed ID：27493499,
PubMed Central ID：PMC4629661 CdSe/ZnS quantum dots conjugated with a fluorescein derivative: a FRET-based pH sensor for physiological alkaline conditions.
Tomokazu Kurabayashi; Nayuta Funaki; Takeshi Fukuda; Shinnosuke Akiyama; Miho Suzuki
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 巻：30, 号：5, 開始ページ：545, 終了ページ：50, 2014年, ［国際誌］
Dual pH-dependent fluorescence peaks from a semiconductor quantum dot (QD) and a pH-dependent fluorescent dye can be measured by irradiating with a single wavelength light, and the pH can be estimated from the ratio of the fluorescent intensity of the two peaks. In this work, ratiometric pH sensing was achieved in an aqueous environment by a fluorescent CdSe/ZnS QD appended with a pH-sensitive organic dye, based on fluorescence resonance energy transfer (FRET). By functionalizing the CdSe/ZnS QD with 5-(and 6)-carboxynaphthofluorescein succinimidyl ester as a pH-dependent fluorescent dye, we succeeded in fabricating sensitive nanocomplexes with a linear response to a broad range of physiological pH levels (7.5-9.5) when excited at 450 nm. We found that a purification process is important for increasing the high-fluorescence intensity ratio of a ratiometric fluorescence pH-sensor, and the fluorescence intensity ratio was improved up to 1.0 at pH 8.0 after the purification process to remove unreacted CdSe/ZnS QDs even though the fluorescence of the dye could not be observed without the purification process. The fluorescence intensity ratio corresponds to the fluorescence intensity of the dye, and this fluorescent dye exhibited pH-dependent fluorescence intensity changes. These facts indicate that the fluorescence intensity ratio linearly increased with increasing pH value of the buffer solution containing the QD and the dye. The FRET efficiencies changed from 0.3 (pH 7.5) to 6.2 (pH 9.5).
英語, 研究論文（学術雑誌）
eISSN：1348-2246, PubMed ID：24813952
2P261 ノロウイルスRNA複製酵素を用いた試験管内RNA淘汰実験から、新奇なdsRNA複製機構が示唆された(20. 生命の起源・進化,ポスター,第52回日本生物物理学会年会(2014年度)) Arai Hidenao; Nishigaki Koichi; Nemoto Naoto; Suzuki Miho; Husimi Yuzuru
生物物理,
巻：54,
号：1,
開始ページ：S238, 2014年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.54.S238_3DOI ID：10.2142/biophys.54.S238_3,
CiNii Articles ID：110009932807 Optimal terminal sequences for continuous or serial isothermal amplification of dsRNA with norovirus RNA replicase Hidenao Arai; Koichi Nishigaki; Naoto Nemoto; Miho Suzuki; HUSIMI Yuzuru
BIOPHYSICS,
巻：10,
開始ページ：15,
終了ページ：23, 2014年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.2142/biophysics.10.15DOI ID：10.2142/biophysics.10.15 Simple preparation of green fluorescent protein conjugated with β-cyclodextrin in a site specific manner.
Miho Suzuki; Yoshihiro Ishimaru; Ayumu Saito; Koichi Nishigaki
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 巻：29, 号：8, 開始ページ：811, 終了ページ：4, 2013年, ［国際誌］
We have site-directedly linked a green fluorescent protein (GFP) variant and a β-cyclodextrin (β-CD) with a simple method to develop a basic complex for sophisticated supramolecules. We have confirmed β-CD grafting on GFP with several methods including matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry (MALDI-TOF MS) without protease digestion and characterized the complex as well. In consideration of the resulting properties, the product we plainly and efficiently obtained could have applications related to sensing devices and drug delivery systems.
英語, 研究論文（学術雑誌）
eISSN：1348-2246, PubMed ID：23934562
1P263 In vitro selection of the preferable 3'-terminal sequences of the template for norovirus RNA replicase(20. Origin of life & Evolution,Poster) Arai Hidenao; Suzuki Miho; Nemoto Naoto; Nishigaki Koichi; Husimi Yuzuru
生物物理,
巻：53,
号：1,
開始ページ：S149, 2013年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.53.S149_4DOI ID：10.2142/biophys.53.S149_4,
CiNii Articles ID：110009819435 Characterization of Norovirus RNA replicase for in vitro amplification of RNA Hidenao Arai; Koichi Nishigaki; Naoto Nemoto; Miho Suzuki; HUSIMI Yuzuru
BMC Biotechnology,
巻：13,
開始ページ：85, 2013年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1186/1472-6750-13-85DOI ID：10.1186/1472-6750-13-85 Simple and tunable Förster resonance energy transfer-based bioprobes for high-throughput monitoring of caspase-3 activation in living cells by using flow cytometry Miho Suzuki; Satoshi Tanaka; Yoichiro Ito; Makiko Inoue; Takafumi Sakai; Koichi Nishigaki
Biochimica et Biophysica Acta - Molecular Cell Research,
巻：1823,
号：2,
開始ページ：215,
終了ページ：226, 2012年02月,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.bbamcr.2011.07.006DOI ID：10.1016/j.bbamcr.2011.07.006,
ISSN：0167-4889,
PubMed ID：21791227,
SCOPUS ID：84862793409 2PT204 cDNA display法による4種類のアミノ酸からなる機能ペプチドの創出(日本生物物理学会第50回年会(2012年度)) Kumachi Shigefumi; Suzuki Miho; Nishigaki Koichi; Husimi Yuzuru; Nemoto Naoto
生物物理,
巻：52,
開始ページ：S139, 2012年
一般社団法人 日本生物物理学会, 英語
DOI：https://doi.org/10.2142/biophys.52.S139_4DOI ID：10.2142/biophys.52.S139_4,
CiNii Articles ID：110009585229 In Vitro Selection of Cathepsin E-Activity-Enhancing Peptide Aptamers at Neutral pH. Madhu Biyani; Masae Futakami; Koichiro Kitamura; Tomoyo Kawakubo; Miho Suzuki; Kenji Yamamoto; Koichi Nishigaki
International journal of peptides,
巻：2011,
開始ページ：834525,
終了ページ：834525, 2011年,
［国際誌］The aspartic protease cathepsin E has been shown to induce apoptosis in cancer cells under physiological conditions. Therefore, cathepsin E-activity-enhancing peptides functioning in the physiological pH range are valuable potential cancer therapeutic candidates. Here, we have used a general in vitro selection method (evolutionary rapid panning analysis system (eRAPANSY)), based on inverse substrate-function link (SF-link) selection to successfully identify cathepsin E-activity-enhancing peptide aptamers at neutral pH. A successive enrichment of peptide activators was attained in the course of selection. One such peptide activated cathepsin E up to 260%, had a high affinity (K(D); ∼300 nM), and had physiological activity as demonstrated by its apoptosis-inducing reaction in cancerous cells. This method is expected to be widely applicable for the identification of protease-activity-enhancing peptide aptamers.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1155/2011/834525DOI ID：10.1155/2011/834525,
PubMed ID：21527983,
PubMed Central ID：PMC3064998 Genome profiling (GP) method based classification of insects: congruence with that of classical phenotype-based one. Shamim Ahmed; Manabu Komori; Sachika Tsuji-Ueno; Miho Suzuki; Akinori Kosaku; Kiyoshi Miyamoto; Koichi Nishigaki
PloS one,
巻：6,
号：8,
開始ページ：e23963, 2011年,
［国際誌］BACKGROUND: Ribosomal RNAs have been widely used for identification and classification of species, and have produced data giving new insights into phylogenetic relationships. Recently, multilocus genotyping and even whole genome sequencing-based technologies have been adopted in ambitious comparative biology studies. However, such technologies are still far from routine-use in species classification studies due to their high costs in terms of labor, equipment and consumables. METHODOLOGY/PRINCIPAL FINDINGS: Here, we describe a simple and powerful approach for species classification called genome profiling (GP). The GP method composed of random PCR, temperature gradient gel electrophoresis (TGGE) and computer-aided gel image processing is highly informative and less laborious. For demonstration, we classified 26 species of insects using GP and 18S rDNA-sequencing approaches. The GP method was found to give a better correspondence to the classical phenotype-based approach than did 18S rDNA sequencing employing a congruence value. To our surprise, use of a single probe in GP was sufficient to identify the relationships between the insect species, making this approach more straightforward. CONCLUSION/SIGNIFICANCE: The data gathered here, together with those of previous studies show that GP is a simple and powerful method that can be applied for actually universally identifying and classifying species. The current success supported our previous proposal that GP-based web database can be constructible and effective for the global identification/classification of species.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1371/journal.pone.0023963DOI ID：10.1371/journal.pone.0023963,
PubMed ID：21912611,
PubMed Central ID：PMC3166070 Novel High-affinity Aβ-binding peptides identified by an advanced in vitro evolution, progressive library method Sachika Tsuji-Ueno; Masayuki Komatsu; Kakeru Iguchi; Masahiro Takahashi; Syuhei Yoshino; Miho Suzuki; Naoto Nemoto; Koichi Nishigaki
Protein and Peptide Letters,
巻：18,
号：6,
開始ページ：642,
終了ページ：650, 2011年,
［査読有り］Bentham Science Publishers B.V., 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.2174/092986611795222678DOI ID：10.2174/092986611795222678,
ISSN：0929-8665,
SCOPUS ID：79953298973 One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA Mochizuki Y; Biyani M; Tsuji-Ueno S; Suzuki M; Nishigaki K; Husimi Y; Nemoto N
ACS Comb.Sci.,
巻：13,
開始ページ：478,
終了ページ：485, 2011年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1021/co2000295DOI ID：10.1021/co2000295 Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer Yasunori Kinoshita; Takahiro Tayama; Koichiro Kitamura; Md Salimullah; Hidekazu Uchida; Miho Suzuki; Yuzuru Husimi; Koichi Nishigaki
BMC BIOTECHNOLOGY,
巻：10, 2010年10月,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1186/1472-6750-10-71DOI ID：10.1186/1472-6750-10-71,
ISSN：1472-6750,
Web of Science ID：WOS:000283064700001 Molecular dynamics study of chemically engineered green fluorescent protein mutants: Comparison of intramolecular fluorescence resonance energy transfer rate Felicity L. Mitchell; Filipp Frank; Gabriel E. Marks; Miho Suzuki; Kenneth T. Douglas; Richard A. Bryce
Proteins: Structure, Function and Bioinformatics,
巻：75,
号：1,
開始ページ：28,
終了ページ：39, 2009年04月,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/prot.22218DOI ID：10.1002/prot.22218,
ISSN：0887-3585,
PubMed ID：18767157,
SCOPUS ID：65249141805 Host-parasite relations of bacteria and phages can be unveiled by Oligostickiness, a measure of relaxed sequence similarity Shamim Ahmed; Ayumu Saito; Miho Suzuki; Naoto Nemoto; Koichi Nishigaki
Bioinformatics,
巻：25,
号：5,
開始ページ：563,
終了ページ：570, 2009年03月,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1093/bioinformatics/btp003DOI ID：10.1093/bioinformatics/btp003,
ISSN：1367-4803,
DBLP ID：journals/bioinformatics/AhmedSSNN09,
PubMed ID：19126576,
SCOPUS ID：61449228484 Rapid in vitro synthesis of pico-mole quantities of peptides Koichiro Kitamura; Chuya Yoshida; Md Salimullah; Yasunori Kinoshita; Miho Suzuki; Naoto Nemoto; Koichi Nishigaki
Chemistry Letters,
巻：37,
号：12,
開始ページ：1250,
終了ページ：1251, 2008年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1246/cl.2008.1250DOI ID：10.1246/cl.2008.1250,
ISSN：0366-7022,
SCOPUS ID：67650481942 Chemically engineered intramolecular FRET mutants of GFP for visualization of versatile cellular events
M. Suzuki; Y. Ito; Y. Oki; Y. Husimi; K. T. Douglas
FEBS Journal, 巻：272, 開始ページ：516, 終了ページ：516, 2005年, ［査読有り］
英語, 研究論文（学術雑誌）
Caspase-3 sensitive signaling in vivo in apoptotic HeLa cells by chemically engineered intramolecular fluorescence resonance energy transfer mutants of green fluorescent protein M. Suzuki; Y. Ito; I. Sakata; T. Sakai; Y. Husimi; K. T. Douglas
Biochemical and Biophysical Research Communications,
巻：330,
号：2,
開始ページ：454,
終了ページ：460, 2005年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.bbrc.2005.02.178DOI ID：10.1016/j.bbrc.2005.02.178,
ISSN：0006-291X,
CiNii Articles ID：80017288069,
PubMed ID：15796904 Protease-sensitive signalling by chemically engineered intramolecular fluorescent resonance energy transfer mutants of green fluorescent protein
Miho Suzuki; Yoichiro Ito; Hannah Elizabeth Savage; Yuzuru Husimi; Kenneth T. Douglas
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 巻：1679, 号：3, 開始ページ：222, 終了ページ：229, 2004年09月, ［査読有り］
英語, 研究論文（学術雑誌）
Intramolecular Fluorescent Resonance Energy Transfer (FRET) by BODIPY Chemical Modification of Cysteine-engineered Mutants of Green Fluorescent Protein
Miho Suzuki; Yoichiro Ito; Elizabeth Savage Hannah; Yuzuru Husimi; Kenneth T. Douglas
Chemistry Letters, 巻：32, 号：3, 開始ページ：306, 終了ページ：307, 2003年02月, ［査読有り］
英語, 研究論文（学術雑誌）
Multipurpose indicator in cells using intramolecular FRET between GFP and chemical compounds
M Suzuki; Y Ito; HE Savage; Y Husimi; KT Douglas
GENETICALLY ENGINEERED AND OPTICAL PROBES FOR BIOMEDICAL APPLICATIONS, 巻：4967, 開始ページ：1, 終了ページ：10, 2003年, ［査読有り］
英語, 研究論文（国際会議プロシーディングス）
ISSN：0277-786X, Web of Science ID：WOS:000184995400001
An Efficient Ligation Method in the Making of an in vitro Virus for in vitro Protein Evolution I. Tabuchi; S. Soramoto; M. Suzuki; K. Nishigaki; N. Nemoto; Y. Husimi
Biol Proced Online,
巻：4,
開始ページ：49,
終了ページ：54, 2002年,
［査読有り］英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1251/bpo33DOI ID：10.1251/bpo33
Generation of Functional Peptides Consisting of Four Types of Amino Acids by a cDNA Display Method
Shigefumi Kumachi; Miho Suzuki; Koichi Nishigaki; Yuzuru Husimi; Naoto Nemoto
巻：21, 開始ページ：86, 終了ページ：87, 2012年08月
英語, 研究発表ペーパー・要旨（国際会議）
ISSN：0961-8368, Web of Science ID：WOS:000307019800091
Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination Miho Suzuki; Yuzuru Husimi; Hirokazu Komatsu; Koji Suzuki; Kenneth T. Douglas
巻：130,
号：17,
開始ページ：5720,
終了ページ：5725, 2008年04月30日
英語
DOI：https://doi.org/10.1021/ja710870eDOI ID：10.1021/ja710870e,
ISSN：0002-7863,
CiNii Articles ID：80019516196,
PubMed ID：18393422,
SCOPUS ID：42649141969 高速分子進化技術eRAPANSY:未来型創薬ツール
西垣功一; 北村幸一郎; 木下保則; 吉田昼也; SALIMULLAH Md; 辻幸香; 上野真吾; MADHU Biyani; 二上雅恵; 高橋陽子; MANISH Biyani; 澁谷昌樹; 武居修; 武居修; 鈴木美穂; 根本直人; 根本直人; MOHAMMED Naimudddin; 二木類; 相田拓洋; 相田拓洋; 内田秀和; 後藤仁志; 山本健二; 草木稔篤; 花田和則; 大関正弘; 伏見譲
生化学, 開始ページ：4P-0180, 2008年
日本語
ISSN：0037-1017, J-Global ID：200902235557435912
3P275 新規in vitro virusの開発(蛋白質(蛋白質工学/進化工学),ポスター発表,第45回日本生物物理学会年会)
新井 秀直; 上野 真吾; 鈴木 美穂; 伏見 譲
生物物理, 巻：47, 号：1, 2007年11月20日
日本生物物理学会, 英語
ISSN：0582-4052, CiNii Articles ID：110006563200
生体内反応モニターバイオプローブの開発と病態診断チップ及びドラッグデリバリーナノデバイスへの応用
鈴木美穂
総合研究機構研究プロジェクト研究成果報告書, 巻：5 (18年度), 開始ページ：191, 終了ページ：192, 2007年
生体内反応モニターバイオプローブの開発と病態診断チップ及びドラッグデリバリーナノデバイスへの応用
鈴木美穂
巻：5 (18年度), 開始ページ：191, 終了ページ：192, 2007年
高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）西垣功一; 吉田昼也; 田山貴紘; 木下保則; 鈴木美穂; 内田秀和; 勝部昭明; 北村幸一郎; 高橋陽子; Md. Salimullah; 門脇知子; 山本健二
埼玉大学地域共同研究センター紀要,
巻：7,
開始ページ：53,
終了ページ：53, 2006年
埼玉大学総合研究機構地域共同研究センター産学連携推進部門, 日本語
ISSN：1347-4758,
CiNii Articles ID：120001371310 高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）西垣 功一; 吉田 昼也; 田山 貴紘; 木下 保則; 鈴木 美穂; 内田 秀和; 勝部 昭明; 北村 幸一郎; 高橋 陽子; Md. Salimullah; 門脇 知子; 山本 健二
埼玉大学地域共同研究センター紀要,
巻：7,
開始ページ：53,
終了ページ：53, 2006年
埼玉大学総合研究機構地域共同研究センター産学連携推進部門, 日本語
ISSN：1347-4758,
CiNii Articles ID：120001371310 3P076 新規in vitro virusの開発(蛋白質 F) 蛋白質工学/進化工学))
新井 秀直; 上野 真吾; 鈴木 美穂; 伏見 譲
生物物理, 巻：45, 号：1, 2005年10月19日
日本生物物理学会, 日本語
ISSN：0582-4052, CiNii Articles ID：110004571740
生体内、生体外共用生命現象可視化バイオプローブの開発と病態診断システムへの応用
鈴木美穂
総合研究機構研究プロジェクト研究成果報告書, 巻：16年度, 2005年
高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）西垣功一; 北村幸一郎; 高橋―本多陽子; 木下保則; 吉田昼也; MD. Salimullah; 鈴木美穂; 内田秀和; 勝部昭明
埼玉大学地域共同研究センター紀要,
巻：6,
開始ページ：83,
終了ページ：83, 2005年
埼玉大学総合研究機構地域共同研究センター産学連携推進部門, 日本語
ISSN：1347-4758,
CiNii Articles ID：120001371347 生体内、生体外共用生命現象可視化バイオプローブの開発と病態診断システムへの応用
鈴木美穂
巻：16年度, 2005年
高速分子進化による高機能バイオ分子の創出（埼玉バイオプロジェクト）西垣 功一; 北村 幸一郎; 高橋—本多 陽子; 木下 保則; 吉田 昼也; MD. Salimullah; 鈴木 美穂; 内田 秀和; 勝部 昭明
埼玉大学地域共同研究センター紀要,
巻：6,
開始ページ：83,
終了ページ：83, 2005年
埼玉大学総合研究機構地域共同研究センター産学連携推進部門, 日本語
ISSN：1347-4758,
CiNii Articles ID：120001371347 3P101 N末端側にリンカーを持つin vitro virusの開発(蛋白質 F) 蛋白質工学/進化工学)
上野 真吾; 深石 圭; 新井 秀直; 鈴木 美穂; 伏見 譲
生物物理, 巻：44, 号：1, 2004年11月10日
日本生物物理学会, 日本語
ISSN：0582-4052, CiNii Articles ID：110001156891
A T-extended vector using a green fluorescent protein as an indicator Yoichiro Ito; Miho Suzuki; Yuzuru Husimi
巻：245,
号：1,
開始ページ：59,
終了ページ：63, 2000年03月07日
英語
DOI：https://doi.org/10.1016/S0378-1119(00)00039-1DOI ID：10.1016/S0378-1119(00)00039-1,
ISSN：0378-1119,
CiNii Articles ID：80011621544,
PubMed ID：10713445,
SCOPUS ID：0034615667 A T-extended vector using a green fluorescent protein as an indicator Y Ito; M Suzuki; Y Husimi
巻：245,
号：1,
開始ページ：59,
終了ページ：63, 2000年03月
英語
DOI：https://doi.org/10.1016/S0378-1119(00)00039-1DOI ID：10.1016/S0378-1119(00)00039-1,
ISSN：0378-1119,
CiNii Articles ID：80011621544,
PubMed ID：10713445,
Web of Science ID：WOS:000085869700007 A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation Yoichiro Ito; Miho Suzuki; Yuzuru Husimi
巻：264,
号：2,
開始ページ：556,
終了ページ：560, 1999年10月22日
英語
DOI：https://doi.org/10.1006/bbrc.1999.1541DOI ID：10.1006/bbrc.1999.1541,
ISSN：0006-291X,
PubMed ID：10529401,
SCOPUS ID：0033595785 A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation Y Ito; M Suzuki; Y Husimi
巻：264,
号：2,
開始ページ：556,
終了ページ：560, 1999年10月
英語
DOI：https://doi.org/10.1006/bbrc.1999.1541DOI ID：10.1006/bbrc.1999.1541,
ISSN：0006-291X,
Web of Science ID：WOS:000083351400042 Adaptive design of protein using virus-like strategy
M. Suzuki; Y. Husimi
FASEB Journal, 巻：11, 号：9, 開始ページ：A1155, 1997年
英語, 研究発表ペーパー・要旨（国際会議）
Production of 1,5-anhydroglucitol from 1,5-anhydrofructose in erythroleukemia cells M Suzuki; S Kametani; K Uchida; H Akanuma
巻：240,
号：1,
開始ページ：23,
終了ページ：29, 1996年08月
英語
DOI：https://doi.org/10.1111/j.1432-1033.1996.0023h.xDOI ID：10.1111/j.1432-1033.1996.0023h.x,
ISSN：0014-2956,
CiNii Articles ID：80009151760,
PubMed ID：8797831,
Web of Science ID：WOS:A1996VE19600003 Production of 1,5-anhydroglucitol from 1,5-anhydrofructose in erythroleukemia cells M Suzuki; S Kametani; K Uchida; H Akanuma
巻：240,
号：1,
開始ページ：23,
終了ページ：29, 1996年08月
英語
DOI：https://doi.org/10.1111/j.1432-1033.1996.0023h.xDOI ID：10.1111/j.1432-1033.1996.0023h.x,
ISSN：0014-2956,
CiNii Articles ID：80009151760,
PubMed ID：8797831,
Web of Science ID：WOS:A1996VE19600003
