Performance information

• Biallelic TEDC1 variants cause a new syndrome with severe growth impairment and endocrine complications
  Noriko Miyake; Kentaro Shiga; Yuya Hasegawa; Chisato Iwabuchi; Kohei Shiroshita; Hiroshi Kobayashi; Keiyo Takubo; Fabien Velilla; Akiteru Maeno; Toshihiro Kawasaki; Yukiko Imai; Noriyoshi Sakai; Tomonori Hirose; Atsushi Fujita; Hidehisa Takahashi; Nobuhiko Okamoto; Mikako Enokizono; Shiho Iwasaki; Shuichi Ito; Naomichi Matsumoto
  European Journal of Human Genetics, Feb. 2025, [Reviewed]
  Springer Science and Business Media LLC, English, Scientific journal
  DOI：https://doi.org/10.1038/s41431-025-01802-3
  DOI ID：10.1038/s41431-025-01802-3, ISSN：1018-4813, eISSN：1476-5438
• PRDM9 drives the location and rapid evolution of recombination hotspots in salmonid fish
  Marie Raynaud; Paola Sanna; Julien Joseph; Julie Clément; Yukiko Imai; Jean-Jacques Lareyre; Audrey Laurent; Nicolas Galtier; Frédéric Baudat; Laurent Duret; Pierre-Alexandre Gagnaire; Bernard de Massy
  PLOS Biology, Volume：23, Number：1, First page：e3002950, Last page：e3002950, Jan. 2025, [Reviewed]
  In many eukaryotes, meiotic recombination occurs preferentially at discrete sites, called recombination hotspots. In various lineages, recombination hotspots are located in regions with promoter-like features and are evolutionarily stable. Conversely, in some mammals, hotspots are driven by PRDM9 that targets recombination away from promoters. Paradoxically, PRDM9 induces the self-destruction of its targets and this triggers an ultra-fast evolution of mammalian hotspots. PRDM9 is ancestral to all animals, suggesting a critical importance for the meiotic program, but has been lost in many lineages with surprisingly little effect on meiosis success. However, it is unclear whether the function of PRDM9 described in mammals is shared by other species. To investigate this, we analyzed the recombination landscape of several salmonids, the genome of which harbors one full-length PRDM9 and several truncated paralogs. We identified recombination initiation sites in Oncorhynchus mykiss by mapping meiotic DNA double-strand breaks (DSBs). We found that DSBs clustered at hotspots positioned away from promoters, enriched for the H3K4me3 and H3K36me3 and the location of which depended on the genotype of full-length Prdm9. We observed a high level of polymorphism in the zinc finger domain of full-length Prdm9, indicating diversification driven by positive selection. Moreover, population-scaled recombination maps in O. mykiss, Oncorhynchus kisutch and Salmo salar revealed a rapid turnover of recombination hotspots caused by PRDM9 target motif erosion. Our results imply that PRDM9 function is conserved across vertebrates and that the peculiar evolutionary runaway caused by PRDM9 has been active for several hundred million years.
  Public Library of Science (PLoS), English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pbio.3002950
  DOI ID：10.1371/journal.pbio.3002950, eISSN：1545-7885
• Meiotic deviations and endoreplication lead to diploid oocytes in female hybrids between bighead catfish (Clarias macrocephalus) and North African catfish (Clarias gariepinus)
  Dmitrij Dedukh; Artem Lisachov; Thitipong Panthum; Worapong Singchat; Yoichi Matsuda; Yukiko Imai; Karel Janko; Kornsorn Srikulnath
  Frontiers in Cell and Developmental Biology, Volume：12, Aug. 2024, [Reviewed]
  Introduction
  
  Reproductive isolation and hybrid sterility are mechanisms that maintain the genetic integrity of species and prevent the introgression of heterospecific genes. However, crosses of closely related species can lead to complex evolution, such as the formation of all-female lineages that reproduce clonally. Bighead catfish (Clarias macrocephalus) and North African catfish (C. gariepinus) diverged 40 million years ago. They are cultivated and hybridized in Thailand for human consumption. Male hybrids are sterile due to genome-wide chromosome asynapsis during meiosis. Although female hybrids are sometimes fertile, their chromosome configuration during meiosis has not yet been studied.
  
  Methods
  
  We analyzed meiosis in the hybrid female catfish at pachytene (synaptonemal complexes) and diplotene (lampbrush chromosomes), using immunostaining to detect chromosome pairing and double-stranded break formation, and FISH with species-specific satellite DNAs to distinguish the parental chromosomes.
  
  Results
  
  More than 95% of oocytes exhibited chromosome asynapsis in female hybrid catfish; however, they were able to progress to the diplotene stage and form mature eggs. The remaining oocytes underwent premeiotic endoreplication, followed by synapsis and crossing over between sister chromosomes, similar to known clonal lineages in fish and reptiles.
  
  Discussion
  
  The occurrence of clonal reproduction in female hybrid catfish suggests a unique model for studying gametogenic alterations caused by hybridization and their potential for asexual reproduction. Our results further support the view that clonal reproduction in certain hybrid animals relies on intrinsic mechanisms of sexually reproducing parental species, given their multiple independent origins with the same mechanism.
  Frontiers Media SA, English, Scientific journal
  DOI：https://doi.org/10.3389/fcell.2024.1465335
  DOI ID：10.3389/fcell.2024.1465335, eISSN：2296-634X
• Establishment of a zebrafish inbred strain, M-AB, capable of regular breeding and genetic manipulation.　　　　　　　　　　　　　　　
  Kenichiro Sadamitsu; Fabien Velilla; Minori Shinya; Makoto Kashima; Yukiko Imai; Toshihiro Kawasaki; Kenta Watai; Miho Hosaka; Hiromi Hirata; Noriyoshi Sakai
  Scientific reports, Volume：14, Number：1, First page：7455, Last page：7455, Mar. 2024, [Reviewed], ［International magazine］
  Inbred strains of organisms are genetically highly uniform and thus useful for life science research. We have previously reported the ongoing generation of the zebrafish IM strain from the India (IND) strain through full sib-pair mating for 16 generations. However, the IM fish laid a small number of offspring and had a short lifespan, implying the need for discreet care in breeding. Here, we report the subsequent establishment of IM strain as well as the generation of a new inbred zebrafish strain, Mishima-AB (M-AB). M-AB was derived from the *AB strain by full sib-pair mating for over 20 generations, which fulfills the general criterion for the establishment of an inbred strain. In contrast to the IM case, maintenance of the M-AB strain by sib-pair mating required almost no special handling. Genome sequencing of IM individuals from the 47th generation and M-AB individuals from the 27th generation revealed that SNP-based genomic heterogeneity across whole-genome nucleotides was 0.008% and 0.011%, respectively. These percentages were much lower than those of the parental IND (0.197%) and *AB (0.086%) strains. These results indicate that the genomes of these inbred strains were highly homogenous. We also demonstrated the successful microinjection of antisense morpholinos, CRISPR/Cas9, and foreign genes into M-AB embryos at the 1-cell stage. Overall, we report the establishment of a zebrafish inbred strain, M-AB, which is capable of regular breeding and genetic manipulation. This strain will be useful for the analysis of genetically susceptible phenotypes such as behaviors, microbiome features and drug susceptibility.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41598-024-57699-3
  DOI ID：10.1038/s41598-024-57699-3, PubMed ID：38548817, PubMed Central ID：PMC10978973
• In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka.　　　　　　　　　　　　　　　
  Kazumasa Takemoto; Toshiya Nishimura; Toshihiro Kawasaki; Yukiko Imai; Karine Levy; Neta Hart; Ivan Olaya; Sean M Burgess; Yaniv M Elkouby; Minoru Tanaka; Noriyoshi Sakai
  Zebrafish, Nov. 2023, [Reviewed], ［International magazine］
  The longevity of sperm in teleost such as zebrafish and medaka is short when isolated even in saline-balanced solution at a physiological temperature. In contrast, some internal fertilizers exhibit the long-term storage of sperm, >10 months, in the female reproductive tract. This evidence implies that sperm in teleost possesses the ability to survive for a long time under suitable conditions; however, these conditions are not well understood. In this study, we show that the sperm of zebrafish can survive and maintain fertility in L-15-based storage medium supplemented with bovine serum albumin, fetal bovine serum, glucose, and lactic acid for 28 days at room temperature. The fertilized embryos developed to normal fertile adults. This storage medium was effective in medaka sperm stored for 7 days at room temperature. These results suggest that sperm from external fertilizer zebrafish and medaka has the ability to survive for at least 4 and 1 week, respectively, in the body fluid-like medium at a physiological temperature. This sperm storage method allows researchers to ship sperm by low-cost methods and to investigate key factors for motility and fertile ability in those sperm.
  English, Scientific journal
  DOI：https://doi.org/10.1089/zeb.2023.0054
  DOI ID：10.1089/zeb.2023.0054, PubMed ID：38010808
• Meiotic Chromosome Dynamics in Zebrafish　　　　　　　　　　　　　　　
  Yukiko Imai; Ivan Olaya; Noriyoshi Sakai; Sean M. Burgess
  Frontiers in Cell and Developmental Biology, Oct. 2021, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
  共同研究・競争的資金等ID：12352557;27956711
• Sycp1 Is Not Required for Subtelomeric DNA Double-Strand Breaks but Is Required for Homologous Alignment in Zebrafish Spermatocytes
  Yukiko Imai; Kenji Saito; Kazumasa Takemoto; Fabien Velilla; Toshihiro Kawasaki; Kei-ichiro Ishiguro; Noriyoshi Sakai
  Frontiers in Cell and Developmental Biology, Volume：9, Mar. 2021, [Reviewed], [Lead]
  In meiotic prophase I, homologous chromosomes are bound together by the synaptonemal complex, in which two axial elements are connected by transverse filaments and central element proteins. In human and zebrafish spermatocytes, homologous recombination and assembly of the synaptonemal complex initiate predominantly near telomeres. In mice, synapsis is not required for meiotic double-strand breaks (DSBs) and homolog alignment but is required for DSB repair; however, the interplay of these meiotic events in the context of peritelomeric bias remains unclear. In this study, we identified a premature stop mutation in the zebrafish gene encoding the transverse filament protein Sycp1. In sycp1 mutant zebrafish spermatocytes, axial elements were formed and paired at chromosome ends between homologs during early to mid-zygonema. However, they did not synapse, and their associations were mostly lost in late zygotene- or pachytene-like stages. In sycp1 mutant spermatocytes, γH2AX signals were observed, and Dmc1/Rad51 and RPA signals appeared predominantly near telomeres, resembling wild-type phenotypes. We observed persistent localization of Hormad1 along the axis in sycp1 mutant spermatocytes, while the majority of Iho1 signals appeared and disappeared with kinetics similar to those in wild-type spermatocytes. Notably, persistent Iho1 foci were observed in spo11 mutant spermatocytes, suggesting that Iho1 dissociation from axes occurs in a DSB-dependent manner. Our results demonstrated that Sycp1 is not required for peritelomeric DSB formation but is necessary for complete pairing of homologs in zebrafish meiosis.
  Frontiers Media SA, English, Scientific journal
  DOI：https://doi.org/10.3389/fcell.2021.664377
  DOI ID：10.3389/fcell.2021.664377, eISSN：2296-634X, 共同研究・競争的資金等ID：12352557;27956711
• PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment
  Yukiko Imai*; Mathilde Biot*; Julie Clément; Mariko Teragaki; Serge Urbach; Thomas Robert; Frédéric Baudat; Corinne Grey; Bernard de Massy; *co-first authors
  eLife, Volume：9, Oct. 2020, [Reviewed], [Lead]
  Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) at specific genomic locations that correspond to PRDM9 binding sites. The molecular steps occurring from PRDM9 binding to DSB formation are unknown. Using proteomic approaches to find PRDM9 partners, we identified HELLS, a member of the SNF2-like family of chromatin remodelers. Upon functional analyses during mouse male meiosis, we demonstrated that HELLS is required for PRDM9 binding and DSB activity at PRDM9 sites. However, HELLS is not required for DSB activity at PRDM9-independent sites. HELLS is also essential for 5-hydroxymethylcytosine (5hmC) enrichment at PRDM9 sites. Analyses of 5hmC in mice deficient for SPO11, which catalyzes DSB formation, and in PRDM9 methyltransferase deficient mice reveal that 5hmC is triggered at DSB-prone sites upon PRDM9 binding and histone modification, but independent of DSB activity. These findings highlight the complex regulation of the chromatin and epigenetic environments at PRDM9-specified hotspots.
  eLife Sciences Publications, Ltd, English, Scientific journal
  DOI：https://doi.org/10.7554/elife.57117
  DOI ID：10.7554/elife.57117, eISSN：2050-084X
• Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes
  Kazumasa Takemoto*; Yukiko Imai*; Kenji Saito; Toshihiro Kawasaki; Peter M. Carlton; Kei-ichiro Ishiguro; Noriyoshi Sakai; *co-first authors
  PLOS Genetics, Volume：16, Number：2, First page：e1008640, Last page：e1008640, Feb. 2020, [Reviewed], [Lead]
  Public Library of Science (PLoS), Scientific journal
  DOI：https://doi.org/10.1371/journal.pgen.1008640
  DOI ID：10.1371/journal.pgen.1008640, eISSN：1553-7404, 共同研究・競争的資金等ID：12352557;27956711
• The PRDM9 KRAB domain is required for meiosis and involved in protein interactions　　　　　　　　　　　　　　　
  Yukiko Imai; Frederic Baudat; Miguel Taillepierre; Marcello Stanzione; Attila Toth; Bernard de Massy
  CHROMOSOMA, Volume：126, Number：6, First page：681, Last page：695, Dec. 2017, [Reviewed], [Lead]
  PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent trimethylation of histone H3 at lysine 4. PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Kruppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. Here, we show that truncation of the KRAB domain of mouse PRDM9 leads to loss of PRDM9 function and altered meiotic prophase and gametogenesis. In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. We also show that CXXC1 interacts with IHO1, an essential component of the meiotic double-strand break (DSB) machinery. As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis.
  SPRINGER, English, Scientific journal
  DOI：https://doi.org/10.1007/s00412-017-0631-z
  DOI ID：10.1007/s00412-017-0631-z, ISSN：0009-5915, eISSN：1432-0886, PubMed ID：28527011, Web of Science ID：WOS:000415626700002
• ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell receptor signaling pathway　　　　　　　　　　　　　　　
  T. Hiratsuka; Y. Takei; R. Ohmori; Y. Imai; M. Ozeki; K. Tamaki; H. Haga; T. Nakamura; T. Tsuruyama
  ONCOGENE, Volume：35, Number：25, First page：3227, Last page：3238, Jun. 2016, [Reviewed]
  ZFP521 was previously identified as a putative gene involved in induction of B-cell lymphomagenesis. However, the contribution of ZFP521 to lymphomagenesis has not been confirmed. In this study, we sought to elucidate the role of ZFP521 in B-cell lymphomagenesis. To this end, we used a retroviral insertion method to show that ZFP521 was a target of mutagenesis in pre-B-lymphoblastic lymphoma cells. The pre-B-cell receptor (pre-BCR) signaling molecules BLNK, BTK and BANK1 were positively regulated by the ZFP521 gene, leading to enhancement of the pre-BCR signaling pathway. In addition, c-myc and c-jun were upregulated following activation of ZFP521. Stimulation of pre-BCR signaling using anti-Vpreb antibodies caused aberrant upregulation of c-myc and c-jun and of Ccnd3, which encodes cyclin D3, thereby inducing the growth of pre-B cells. Stimulation with Vpreb affected the growth of pre-B cells, and addition of interleukin (IL)-7 receptor exerted competitive effects on pre-B-cell growth. Knockdown of BTK and BANK1, targets of ZFP521, suppressed the effects of Vpreb stimulation on cell growth. Furthermore, in human lymphoblastic lymphoma, analogous to pre-B-cell lymphoma in mice, the expression of ZNF521, the homolog of ZFP521 in humans, was upregulated. In conclusion, our data showed that the ZFP521 gene comprehensively induced pre-B-cell lymphomagenesis by modulating the pre-B-cell receptor signaling pathway.
  NATURE PUBLISHING GROUP, English, Scientific journal
  DOI：https://doi.org/10.1038/onc.2015.385
  DOI ID：10.1038/onc.2015.385, ISSN：0950-9232, eISSN：1476-5594, Web of Science ID：WOS:000378306400002
• Septal localization by membrane targeting sequences and a conserved sequence essential for activity at the COOH-terminus of Bacillus subtilis cardiolipin synthase　　　　　　　　　　　　　　　
  Jin Kusaka*; Satoshi Shuto*; Yukiko Imai*; Kazuki Ishikawa; Tomo Saito; Kohei Natori; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto; *co-first authors
  RESEARCH IN MICROBIOLOGY, Volume：167, Number：3, First page：202, Last page：214, Apr. 2016, [Reviewed], [Lead]
  The acidic phospholipid cardiolipin (CL) is localized on polar and septal membranes and plays an important physiological role in Bacillus subtilis cells. ClsA, the enzyme responsible for CL synthesis, is also localized on septal membranes. We found that GFP fusion proteins of the enzyme with NH2-terminal and internal deletions retained septal localization. However, derivatives with deletions starting from the COOH-terminus (Leu482) ceased to localize to the septum once the deletion passed the Ile residue at 448, indicating that the sequence responsible for septal localization is confined within a short distance from the COOH-terminus. Two sequences, Ile436-Leu450 and Leu466-Leu478, are predicted to individually form an amphipathic a-helix. This configuration is known as a membrane targeting sequence (MTS) and we therefore refer to them as MTS2 and MTS1, respectively. Either one has the ability to affect septal localization, and each of these sequences by itself localizes to the septum. Membrane association of the constructs of this enzyme containing the MTSs was verified by subcellular fractionation of the cells. CL synthesis, in contrast, was abolished after deleting just the last residue, Leu482, in the COOH-terminal four amino acid residue sequence, Ser-Pro-Ile-Leu, which is highly conserved among bacterial CL synthases. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
  ELSEVIER SCIENCE BV, English, Scientific journal
  DOI：https://doi.org/10.1016/j.resmic.2015.11.004
  DOI ID：10.1016/j.resmic.2015.11.004, ISSN：0923-2508, eISSN：1769-7123, PubMed ID：26708983, Web of Science ID：WOS:000372890100006
• Meiotic recombination in mammals: localization and regulation　　　　　　　　　　　　　　　
  Frederic Baudat; Yukiko Imai; Bernard de Massy
  NATURE REVIEWS GENETICS, Volume：14, Number：11, First page：794, Last page：806, Nov. 2013, [Reviewed]
  During meiosis, a programmed induction of DNA double-strand breaks (DSBs) leads to the exchange of genetic material between homologous chromosomes. These exchanges increase genome diversity and are essential for proper chromosome segregation at the first meiotic division. Recent findings have highlighted an unexpected molecular control of the distribution of meiotic DSBs in mammals by a rapidly evolving gene, PR domain-containing 9 (PRDM9), and genome-wide analyses have facilitated the characterization of meiotic DSB sites at unprecedented resolution. In addition, the identification of new players in DSB repair processes has allowed the delineation of recombination pathways that have two major outcomes, crossovers and non-crossovers, which have distinct mechanistic roles and consequences for genome evolution.
  NATURE PUBLISHING GROUP, English
  DOI：https://doi.org/10.1038/nrg3573
  DOI ID：10.1038/nrg3573, ISSN：1471-0056, eISSN：1471-0064, PubMed ID：24136506, Web of Science ID：WOS:000325923900011
• Development, evaluation, and application of forensic mathematical analysis program in mixture evidence　　　　　　　　　　　　　　　
  TAKEUCHI Haruya; TORISU Yoko; MIYAO Masashi; HORI Motohide; IMAI Yukiko; KOTANI Hirokazu; TAMAKI Keiji
  Volume：19, First page：235, Last page：239, May 2011
  Japanese
  CiNii Articles ID：10029668935, CiNii Books ID：AA11948366
• アリルの個数(NA)を利用した混合試料における関与人数の推定　　　　　　　　　　　　　　　
  竹内 晴哉; 真鍋 翔; 今井 裕紀子; 木田 佳枝; 玉木 敬二
  Volume：65, Number：1, First page：88, Last page：88, May 2011
  Japanese
  ISSN：0047-1887, 医中誌Web ID：2011277180
• Murine Leukemia Retrovirus Integration Induces the Formation of Transcription Factor Complexes on Palindromic Sequences in the Signal Transducer and Activator of Transcription Factor 5a Gene During the Development of Pre-B Lymphomagenesis　　　　　　　　　　　　　　　
  Tatsuaki Tsuruyama; Takuya Hiratsuka; Guang Jin; Yukiko Imai; Haruya Takeuchi; Yasuhiro Maruyama; Kazuya Kanaya; Munetaka Ozeki; Tetsuya Takakuwa; Hironori Haga; Keiji Tamaki; Takuro Nakamura
  AMERICAN JOURNAL OF PATHOLOGY, Volume：178, Number：3, First page：1374, Last page：1386, Mar. 2011, [Reviewed]
  Murine leukemia retrovirus (MLV) vectors are highly effective tools for introducing a foreign gene into a target host genome. However, it remains unclear how integrated retroviral promoter activity is influenced by the upstream or downstream sequences and how the host cell phenotype is influenced by the integrated promoter activity. Herein, we analyzed a set of pre-B lymphoma clones in which the MLV genome was integrated into the signal transducer and activator of transcription factor 5a (Stat5a) gene. Among the clones, the lymphoma clones with a provirus integrating into the middle position of the palindromic target sequences showed significantly higher transcription of the Stat5a gene; and p300 and other transcriptional factors formed complexes, with binding to the proviral-host junctional DNA segment. By using a luciferase assay, the upstream and downstream sequences of the provirus contributed to the up-regulation of proviral promoter activity. In concomitance with the higher Stat5a transcription, the immunoglobulin gene recombination was arrested. Antiapoptotic activity was significantly higher, with an increase in Bcl-xL, one of the targets of STAT5A, when IL-7 was supplied. Thus, a minute difference between MLV integration sites can lead to Large differences in the host phenotype through the formation of transcription factor complexes on the proviral-host junctional DNA segment, suggesting that caution is necessary in monitoring integration sites when working with MLV vectors. (Am J Pathol 2011, 178:1374-1386; DOI: 10.1016/j.ajpath.2010.12.012)
  ELSEVIER SCIENCE INC, English, Scientific journal
  DOI：https://doi.org/10.1016/j.ajpath.2010.12.012
  DOI ID：10.1016/j.ajpath.2010.12.012, ISSN：0002-9440, PubMed ID：21356387, Web of Science ID：WOS:000288185600043
• Bリンパ芽球性白血病/リンパ腫におけるzfp521遺伝子発現異常と関連シグナル経路解析　　　　　　　　　　　　　　　
  木田 佳枝; 今井 裕紀子; 尾関 宗孝; 小谷 泰一; 中村 卓郎; 羽賀 博典; 玉木 敬二; 鶴山 竜昭
  Volume：100, Number：1, First page：325, Last page：325, Mar. 2011
  Japanese
  ISSN：0300-9181, 医中誌Web ID：2011243014
• JAK2/STAT5活性化による急性リンパ腫・白血病発症モデル　　　　　　　　　　　　　　　
  鶴山 竜昭; 木田 佳枝; 今井 裕紀子; 玉木 敬二; 羽賀 博典
  Volume：100, Number：1, First page：325, Last page：325, Mar. 2011
  Japanese
  ISSN：0300-9181, 医中誌Web ID：2011243018
• Zfp遺伝子へのマウス白血病レトロウイルス挿入を伴うプレB細胞リンパ腫の発症(Maturation arrest and development of spontaneous pre-B cell lymphoma with MLV integration into the zfp521)　　　　　　　　　　　　　　　
  竹内 晴哉; 今井 裕紀子; 尾関 宗孝; 玉木 敬二; 鶴山 竜昭
  日本癌学会総会記事, Volume：69回, First page：114, Last page：114, Aug. 2010
  (一社)日本癌学会, English
  ISSN：0546-0476, 医中誌Web ID：2013262910
• 前駆B細胞芽球型リンパ腫におけるIL-7シグナル伝達経路の新しいシグナル物質Fiz1とHipk2(Fiz1 and Hipk2 are involved in the IL-7 signaling pathway in B lymphoblastic lymphomas)　　　　　　　　　　　　　　　
  鶴山 竜昭; 今井 裕紀子; 竹内 晴哉; 尾関 宗孝; 中村 卓郎; 日合 弘; 玉木 敬二
  日本癌学会総会記事, Volume：69回, First page：163, Last page：163, Aug. 2010
  (一社)日本癌学会, English
  ISSN：0546-0476, 医中誌Web ID：2013263160
• Dual retrovirus integration tagging: identification of new signaling molecules Fiz1 and Hipk2 that are involved in the IL-7 signaling pathway in B lymphoblastic lymphomas　　　　　　　　　　　　　　　
  Tatsuaki Tsuruyama; Yukiko Imai; Haruya Takeuchi; Takuya Hiratsuka; Yasuhiro Maruyama; Kazuya Kanaya; Richard Kaszynski; Guang Jin; Tomoko Okuno; Munetaka Ozeki; Takuro Nakamura; Tetsuya Takakuwa; Toshiaki Manabe; Keiji Tamaki; Hiroshi Hiai
  JOURNAL OF LEUKOCYTE BIOLOGY, Volume：88, Number：1, First page：107, Last page：116, Jul. 2010, [Reviewed]
  IL-7R, FLT3, and CD43 are surface antigens expressed during the transition from pro-B to pre-B cells in BM. To understand interactions between their signaling pathways, we analyzed spontaneous mouse B-LBLs with dual MLV integration into Stat5a and Fiz1 or Stat5a and Hipk2. MLV integration resulted in up-regulation of these genes in lymphoma cells compared with normal pro-B cells from the BM. In lymphomas with both integrations into Stat5a and Fiz1, increases in phosphorylated STAT5A and expression of c-Myc, a target gene of STAT5A, were observed following stimulation of the FLT3. Clones with the dual integrations grew faster in IL-7 and FLT3L-supplemented medium than clones with Stat5a integration alone. On the other hand, in lymphomas with integrations into Stat5a and Hipk2, increases in phosphorylated STAT5A and expression of c-Myc were observed following cross- linking of CD43. In conclusion, FLT3 and CD43 signaling pathways involve STAT5A via Fiz1 and Hipk2 in B-LBLs. Identification of the dual MLV integration sites in B-LBLs, therefore, will provide an excellent tool for identification of the signaling pathways in B-LBLs. J. Leukoc. Biol. 88: 107-116; 2010.
  FEDERATION AMER SOC EXP BIOL, English, Scientific journal
  DOI：https://doi.org/10.1189/jlb.1109748
  DOI ID：10.1189/jlb.1109748, ISSN：0741-5400, PubMed ID：20360400, Web of Science ID：WOS:000279356700012
• A quantitative trait locus responsible for inducing B-cell lymphoblastic lymphoma is a hotspot for microsatellite instability　　　　　　　　　　　　　　　
  TSURUYAMA Tatsuaki; KASZYNSKI Richard H; IMAI Yukiko; TAKEUCHI Haruya; OZEKI Munetaka; OKUNO Tomoko; TAMAKI Keiji; HIRATSUKA Takuya; HIAI Hiroshi
  Volume：18, First page：227, Last page：232, May 2010
  English
  CiNii Articles ID：10029668345, CiNii Books ID：AA11948366
• Evi3高発現による前駆Bリンパ芽球型リンパ腫発症モデルマウスとしてのSL/Kh　　　　　　　　　　　　　　　
  今井 裕紀子; 竹内 晴哉; 平塚 拓也; 尾関 宗孝; 奥野 知子; 中村 卓郎; 高桑 徹也; 真鍋 敏明; 日合 弘; 玉木 敬二; 鶴山 竜昭
  Volume：99, Number：1, First page：217, Last page：217, Mar. 2010
  Japanese
  ISSN：0300-9181, 医中誌Web ID：2010254366

• Septal localization by membrane targeting sequences at the COOH terminus of Bacillus subtilis cardiolipin synthase　　　　　　　　　　　　　　　
  Yukiko Imai; Satoshi Matsuoka; Hiroshi Hara; Kouji Matsumoto
  GENES & GENETIC SYSTEMS, Volume：91, Number：6, First page：380, Last page：380, Dec. 2016, [Reviewed]
  GENETICS SOC JAPAN, English, Summary international conference
  ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000405886000245
• ウイルス性リンパ腫発症に関与する遺伝子evi3のプロモーター解析.　　　　　　　　　　　　　　　
  今井裕紀子; 鶴山竜昭; 竹内晴哉; 玉木敬二
  2010
  Summary national conference
• 先天性胆道閉鎖症の一剖検例.　　　　　　　　　　　　　　　
  宮尾 昌; 阿比留 仁; 鳥巣陽子; 今井裕紀子; 尾関宗孝; 小谷泰一; 玉木敬二
  2010
  Summary national conference
• リンパ腫ゲノムにおけるマイクロサテライト多型不安定性集積の空間統計学解析.　　　　　　　　　　　　　　　
  鶴山竜昭; リチャード・カシンスキー; 赤塚慎也; 今井裕紀子; 竹内晴哉; 尾関宗孝; 奥野知子; 豊國伸哉; 玉木敬二
  Volume：64, Number：1, First page：54, Last page：54, 2010
  Japanese, Summary national conference
  ISSN：0047-1887, 医中誌Web ID：2010243860
• Reye 症候群の可能性が示唆された一剖検例.　　　　　　　　　　　　　　　
  鳥巣陽子; 宮尾 昌; 阿比留 仁; 今井裕紀子; 奥野知子; 尾関宗孝; 鶴山竜昭; 玉木敬二
  2009
  Summary national conference

• Initiation of Meiotic Recombination in Zebrafish Males　　　　　　　　　　　　　　　
  Yukiko Imai; Julie Clement
  The 12th 3R+3C International Symposium, Nov. 2024
  Nov. 2024 - Nov. 2024, English, Oral presentation
  共同研究・競争的資金等ID：33962239;37011963;27956711;12352557
• Initiation of meiotic recombination in zebrafish males　　　　　　　　　　　　　　　
  Yukiko Imai
  Sep. 2024
  Sep. 2024 - Sep. 2024, Japanese, Oral presentation
  共同研究・競争的資金等ID：33962239;12352557;27956711
• Initiation of meiotic recombination in zebrafish males　　　　　　　　　　　　　　　
  Yukiko Imai
  The 18th International Zebrafish Conference, Aug. 2024
  Aug. 2024 - Aug. 2024, English, Oral presentation
  共同研究・競争的資金等ID：33962239;27956711;12352557
• “Meiotic Recombination in telomere bouquet-defective zebrafish”　　　　　　　　　　　　　　　
  Yukiko Imai
  The 46th Annual Meeting of the Molecular Biology Society of Japan・Symposium「Diverse mechanisms and principle of meiotic recombination among multiple organisms」, Nov. 2023
  共同研究・競争的資金等ID：33962239;12352557;27956711
• ゼブラフィッシュの減数分裂組換えにおける核膜-テロメア接着　　　　　　　　　　　　　　　
  今井裕紀子
  Mar. 2023, [Invited]
  Mar. 2023 - Mar. 2023, Japanese
  共同研究・競争的資金等ID：27956711;12352557;37011963
• Initiation of meiotic recombination in zebrafish　　　　　　　　　　　　　　　
  Yukiko Imai
  RIKEN BDR Women and Future in Science Seminar, Jan. 2023, [Invited]
  Japanese, Public discourse
  共同研究・競争的資金等ID：33962239;37011963;27956711;12352557
• ゼブラフィッシュからみる組換え開始のホットスポット　　　　　　　　　　　　　　　
  今井裕紀子
  Oct. 2022, [Invited]
  Oct. 2022 - Oct. 2022, Japanese, Invited oral presentation
  共同研究・競争的資金等ID：33962239;37011963;27956711
• Disruption of the nuclear envelope-telomere attachment in zebrafish meiosis　　　　　　　　　　　　　　　
  Yukiko Imai
  1st Annual Symposium on Meiotic Chromosome Dynamics in Zebrafish, Sep. 2022
  Sep. 2022 - Sep. 2022, English, Oral presentation
  共同研究・競争的資金等ID：12352557;27956711
• ゼブラフィッシュ減数分裂における組換え開始　　　　　　　　　　　　　　　
  今井裕紀子
  Jul. 2022, [Invited]
  Jul. 2022 - Jul. 2022, Japanese, Invited oral presentation
  共同研究・競争的資金等ID：33962239;27956711;12352557
• ゼブラフィッシュの減数分裂における染色体末端指向的な組換え　　　　　　　　　　　　　　　
  今井裕紀子
  Jul. 2022, [Invited]
  Jul. 2022 - Jul. 2022, Japanese, Invited oral presentation
  共同研究・競争的資金等ID：27956711;33962239;12352557
• Initiation of meiotic recombination in zebrafish　　　　　　　　　　　　　　　
  Yukiko Imai
  Apr. 2022
  Apr. 2022 - Apr. 2022, English, Invited oral presentation
• ゼブラフィッシュからアプローチする減数分裂期のテロメア指向的な組換え開始　　　　　　　　　　　　　　　
  今井 裕紀子
  Mar. 2022, [Invited]
  Japanese, Others
  共同研究・競争的資金等ID：27956711;12352557
• Sycp1 is not required for subtelomeric DNA double-strand breaks but is required for homologous alignment in zebrafish spermatocytes.　　　　　　　　　　　　　　　
  Yukiko Imai; Noriyoshi Sakai
  The Students and Postdocs Meiosis Workshop, v3.0 – PDSM 2021, Apr. 2021
  Poster presentation
  共同研究・競争的資金等ID：12352557;27956711
• The synaptonemal complex and recombination in zebrafish meiosis　　　　　　　　　　　　　　　
  Yukiko Imai; Noriyoshi Sakai
  Jan. 2021
  Poster presentation
• The synaptonemal complex and recombination in zebrafish meiosis　　　　　　　　　　　　　　　
  Yukiko IMAI
  The 43th Annual Meeting of the Molecular Biology Society of Japan,, Dec. 2020, [Invited]
  Dec. 2020 - Dec. 2020, English, Nominated symposium
  共同研究・競争的資金等ID：27956711;12352557;27956815
• Sycp2 is essential for early meiotic events in zebrafish spermatocytes　　　　　　　　　　　　　　　
  Yukiko Imai
  Meiosis In Quarantine, Jun. 2020
  English, Others
  共同研究・競争的資金等ID：12352557;27956711;27956815
• ゼブラフィッシュをモデルとした減数分裂期相同組換えの開始メカニズムへのアプローチ　　　　　　　　　　　　　　　
  今井裕紀子
  Dec. 2019, [Invited]
  Dec. 2019 - Dec. 2019, Japanese
  共同研究・競争的資金等ID：12352557;27956711
• ゼブラフィッシュ精母細胞の相同組換えはSycp2 に依存してテロメア近傍で始まる　　　　　　　　　　　　　　　
  今井裕紀子; 酒井則良
  Mar. 2019
  Mar. 2019 - Mar. 2019, Japanese, Poster presentation
  共同研究・競争的資金等ID：12352557
• ゼブラフィッシュ精子形成における相同組換えは Sycp2 に依存してテロメア近傍で始まる　　　　　　　　　　　　　　　
  今井裕紀子; 酒井則良
  Jan. 2019
  Jan. 2019 - Jan. 2019, Japanese, Oral presentation
  共同研究・競争的資金等ID：12352557
• Initiation of meiotic recombination in mice: search for PRDM9 partners　　　　　　　　　　　　　　　
  Yukiko IMAI; Bernard; DE MASSY
  Jan. 2017
  Jan. 2017 - Jan. 2017, English, Oral presentation
• Initiation of meiotic recombination in mice: search for PRDM9 partners　　　　　　　　　　　　　　　
  Yukiko IMAI; Bernard; DE MASSY
  Students and Postdocs Meiosis Workshop, Sep. 2016
  Sep. 2016 - Sep. 2016, English, Oral presentation

• Aug. 2024 - Present, THE GENETICS SOCIETY OF JAPAN

• Analysis of sexual differences in zebrafish meiosis　　　　　　　　　　　　　　　
  JSPS, KAKENHI, Grant-in-Aid for Scientific Research(C), Apr. 2024 - Mar. 2027
  Yukiko Imai, NAtional Institute of Genetics
  Grant amount(Total)：4680000, Direct funding：3600000, Indirect funding：1080000
  Grant number：24K09477
• Initiation of meiotic recombination: approach to nuclear dynamics in zebrafish　　　　　　　　　　　　　　　
  Japan Science and Technology Agency, PRESTO, Oct. 2021 - Apr. 2025
  Yukiko Imai, National Institute of Genetics, Principal investigator
  講演・口頭発表等ID：49152497, 受賞ID：49152478, 学術貢献活動ID：46906328
• Molecular basis for subtelomeric recombination in meiosis　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Apr. 2022 - Mar. 2024
  Yukiko Imai, National Institute of Genetics, Principal investigator
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  Grant number：22K15129
  講演・口頭発表等ID：49152497, 受賞ID：49152478
• ゼブラフィッシュ精子形成の組換えにおけるDNA二重鎖切断因子の同定　　　　　　　　　　　　　　　
  Apr. 2019 - Mar. 2022
  Principal investigator
  講演・口頭発表等ID：32003434, 受賞ID：33692302
• The specification of meiotic recombination sites: chromosomal structures, genome and epigenome　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists, Apr. 2019 - Mar. 2022
  Imai Yukiko, National Institute of Genetics, Principal investigator
  Grant amount(Total)：4030000, Direct funding：3100000, Indirect funding：930000
  In human and zebrafish males, meiotic recombination occurs preferentially near the ends of chromosomes. However, how recombination is induced in these regions remains largely unknown. In this project, mutant zebrafish lines deficient for meiotic chromosomal structures were examined for their phenotypes in meiotic recombination. In addition, genome-wide mapping of recombination initiation sites and a histone modification identified characteristic features of recombination sites.
  Grant number：19K16045
  論文ID：33961040, 講演・口頭発表等ID：49152497, 受賞ID：49152478
• Regulations of Initiation of Meiotic Recombination by Chromosomal Structures　　　　　　　　　　　　　　　
  JSPS, Grant-in-Aid for Research Activity Start-up, Apr. 2018 - Mar. 2020
  Yukiko Imai, National Institute of Genetics, Principal investigator
  Competitive research funding
  論文ID：33961040, 講演・口頭発表等ID：49152497, 受賞ID：49152478
• Structure and function of bacterial lipid domains　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2012 - 31 Mar. 2016
  MATSUMOTO Kouji; HARA Hiroshi; MATSUOKA Satoshi; SADAIE Yoshito; HASHIMOTO Michihiro; KUSAKA Jin; SHUTO Satoshi; ISHIKAWA Kazuki; IMAI Yukiko; TANIGUCHUI Aya; ITOU Aya; MIYAGAWA Hiroyoshi; UMEKAWA Mitsuru; KONDO Daitetsu; SEYA Manato; MINESHIMA Ryota; MIYAMATSU Saori; MATSUSHIMA Wakana; SEKI Takahiro; NISHINO Yuki; FURUKAWA Yugo; SAITO Tomo; NATORI Kohei, Saitama University
  Grant amount(Total)：5590000, Direct funding：4300000, Indirect funding：1290000
  To clarify the structure and function of bacterial lipid domains, we have adopted following two approaches. i) To elucidate the mechanism of formation of cardiolipin domain in Bacillus subtilis cells, the function of C-terminal α-helices of cardiolipin synthase is examined for septal membrane localization by fluorescence microscopy and Western blotting using GFP-ClsA fusion proteins. The enzyme is shown to be septally localized by means of its C-terminal α-helices, indicating that the C-terminal α-helices of the enzyme have a function of membrane targeting. ii) B. subtilis MinD is examined for septal localization in minJ mutant cells and is shown to be septally localized by means of the membrane targeting sequence at its C-terminus. This indicates that a correction in the current model of the sequential interaction for MinD binding to septal membranes is required.
  Grant number：24570003
• Structure and formation of bacterial lipid domains　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 2009 - 2011
  MATSUMOTO Kouji; HARA Hiroshi; MATSUOKA Satoshi, Saitama University
  Grant amount(Total)：4940000, Direct funding：3800000, Indirect funding：1140000
  Cardiolipin and phosphatidylethanolamine are localized in the septal and polar membranes, not distributed homogeneously, in Bacillus subtilis cells. In order to clarify the structure and mechanism of formation of bacterial lipid domains, we have adopted following two approaches. (i) To examine the distribution of other membrane lipids, we have screened a library of oligopeptides with random amino acid sequence to develop new probes for specific detection of the other lipids. (ii) To study the mechanism of lipid domain formation, the means to localize septally of lipid synthases and MinD, which regulates the position of cell division, is examined with special focus on the region at its COOH-terminus.
  Grant number：21570002

• 第５回有性生殖研究会　　　　　　　　　　　　　　　
  Planning etc
  06 Mar. 2025 - 08 Mar. 2025
  Academic society etc
• Fish and Meiosis　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  Yukiko Imai, 12 Dec. 2023 - 12 Dec. 2023
  Competition etc
• Diverse mechanisms and principle of meiotic recombination among multiple organisms, MBSJ 2023　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  Masaru Ito, Yukiko Imai, 29 Nov. 2023 - 29 Nov. 2023
  Competition etc
  33962239
• 1st Annual Symposium on Meiotic Chromosome Dynamics in Zebrafish　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  Sean M Burgess, Yukiko Imai, Aurora Ruiz-Herrera, 14 Sep. 2022
  Academic society etc
• 2022 遺伝研研究会 (熊大発生研共催) 「有性生殖における染色体・クロマチン・核動態に関する若手研究者の会」　　　　　　　　　　　　　　　
  Planning etc, Panel chair etc
  14 Apr. 2022 - 15 Apr. 2022
  Academic society etc