Performance information

• A macroscopically homogeneous lipid phase exhibits leaflet-specific lipid diffusion in a glass-supported lipid bilayer
  Takuhiro Otosu; Miyuki Sakaguchi; Shoichi Yamaguchi
  Physical Chemistry Chemical Physics, Volume：27, Number：9, First page：4944, Last page：4949, Feb. 2025, [Reviewed]
  Leaflet-specific lipid diffusion analysis revealed that the strength of interleaflet coupling is sensitive to the lipid composition even in the region where macroscopically homogeneous phase is observed.
  Royal Society of Chemistry (RSC), Scientific journal
  DOI：https://doi.org/10.1039/d5cp00203f
  DOI ID：10.1039/d5cp00203f, ISSN：1463-9076, eISSN：1463-9084
• Peripheral adsorption of polylysine on one leaflet of a lipid bilayer reduces the lipid diffusion of both leaflets　　　　　　　　　　　　　　　
  Kosei Shimizu; Miyuki Sakaguchi; Shoichi Yamaguchi; Takuhiro Otosu
  Physical Chemistry Chemical Physics, Feb. 2024, [Reviewed]
  Leaflet-specific lipid diffusion analysis revealed that polylysine adsorption on one leaflet of a lipid bilayer is sensed by the opposite leaflet.
  English, Scientific journal
  DOI：https://doi.org/10.1039/d3cp04882a
  DOI ID：10.1039/d3cp04882a, ISSN：1463-9076, ORCID：178157859
• Raman spectroscopy of isotopically pure and diluted high‐ and low‐density amorphous ices
  Senri Ishihara; Tetsuyuki Takayama; Miyuki Sakaguchi; Takuhiro Otosu; Takuma Yagasaki; Yoshiharu Suzuki; Shoichi Yamaguchi
  Journal of Raman Spectroscopy, Volume：53, Number：10, First page：1773, Last page：1784, Feb. 2022, [Reviewed]
  Abstract
  
  We present an experimental and theoretical study of Raman spectroscopy of isotopically pure and diluted amorphous as well as crystalline ices at 30 K. Our experiments comprise polarized (VV) and depolarized (VH) spectra in the OH‐stretch region, whereas our calculations involve molecular dynamics (MD) simulation of the high‐ and low‐density amorphous ices through vitrification and subsequent formulation of vibrational Hamiltonian of coupled OH oscillators in the hydrogen‐bond network of the ices. Our theoretical Raman VV and VH spectra at each isotopic dilution are in good agreement with the experimental data. This allows us not only to carry out detailed spectral assignments for the amorphous and crystalline ices from the viewpoint of vibrational phase, delocalization, and density of states but also to confirm the reliability of the MD simulation in which deeply supercooled liquid water spontaneously separates into high‐ and low‐density fluid phases below a liquid–liquid critical point.
  Wiley, Scientific journal
  DOI：https://doi.org/10.1002/jrs.6322
  DOI ID：10.1002/jrs.6322, ISSN：0377-0486, eISSN：1097-4555
• Catalytic mechanism of the tyrosinase reaction toward the Tyr ⁹⁸ residue in the caddie protein
  Matoba, Y.; Kihara, S.; Bando, N.; Yoshitsu, H.; Sakaguchi, M.; Kayama, K.; Yanagisawa, S.; Ogura, T.; Sugiyama, M.
  PLoS Biology, Volume：16, Number：12, 2018, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1371/journal.pbio.3000077
  DOI ID：10.1371/journal.pbio.3000077, ISSN：1545-7885, ORCID：178157613, SCOPUS ID：85059288822
• Importance of the Reactant-State Potentials of Chromium(V)-Oxo Complexes to Determine the Reactivity in Hydrogen-Atom Transfer Reactions
  Kotani, H.; Kaida, S.; Ishizuka, T.; Mieda, K.; Sakaguchi, M.; Ogura, T.; Shiota, Y.; Yoshizawa, K.; Kojima, T.
  Inorganic Chemistry, Volume：57, Number：21, 2018, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1021/acs.inorgchem.8b02453
  DOI ID：10.1021/acs.inorgchem.8b02453, ISSN：1520-510X, ORCID：178157612, SCOPUS ID：85055829671
• A nanosecond time-resolved XFEL analysis of structural changes associated with CO release from cytochrome C oxidase
  Shimada, A.; Kubo, M.; Baba, S.; Yamashita, K.; Hirata, K.; Ueno, G.; Nomura, T.; Kimura, T.; Shinzawa-Itoh, K.; Baba, J.; Hatano, K.; Eto, Y.; Miyamoto, A.; Murakami, H.; Kumasaka, T.; Owada, S.; Tono, K.; Yabashi, M.; Yamaguchi, Y.; Yanagisawa, S.; Sakaguchi, M.; Ogura, T.; Komiya, R.; Yan, J.; Yamashita, E.; Yamamoto, M.; Ago, H.; Yoshikawa, S.; Tsukihara, T.
  Science Advances, Volume：3, Number：7, 2017, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1126/sciadv.1603042
  DOI ID：10.1126/sciadv.1603042, ISSN：2375-2548, ORCID：178157615, SCOPUS ID：85034426685
• Activation Mechanism of the Streptomyces Tyrosinase Assisted by the Caddie Protein
  Matoba, Y.; Kihara, S.; Muraki, Y.; Bando, N.; Yoshitsu, H.; Kuroda, T.; Sakaguchi, M.; Kayama, K.; Tai, H.; Hirota, S.; Ogura, T.; Sugiyama, M.
  Biochemistry, Volume：56, Number：41, 2017, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1021/acs.biochem.7b00635
  DOI ID：10.1021/acs.biochem.7b00635, ISSN：1520-4995, ORCID：178157610, SCOPUS ID：85031708852
• A nearly on-axis spectroscopic system for simultaneously measuring UV–visible absorption and X-ray diffraction in the SPring-8 structural genomics beamline
  Miyuki Sakaguchi; Tetsunari Kimura; Takuma Nishida; Takehiko Tosha; Hiroshi Sugimoto; Yoshihiro Yamaguchi; Sachiko Yanagisawa; Go Ueno; Hironori Murakami; Hideo Ago; Masaki Yamamoto; Takashi Ogura; Yoshitsugu Shiro; Minoru Kubo
  Journal of Synchrotron Radiation, Volume：23, Number：1, First page：334, Last page：338, Jan. 2016, [Reviewed]
  UV–visible absorption spectroscopy is useful for probing the electronic and structural changes of protein active sites, and thus the on-line combination of X-ray diffraction and spectroscopic analysis is increasingly being applied. Herein, a novel absorption spectrometer was developed at SPring-8 BL26B2 with a nearly on-axis geometry between the X-ray and optical axes. A small prism mirror was placed near the X-ray beamstop to pass the light only 2° off the X-ray beam, enabling spectroscopic analysis of the X-ray-exposed volume of a crystal during X-ray diffraction data collection. The spectrometer was applied to NO reductase, a heme enzyme that catalyzes NO reduction to N₂O. Radiation damage to the heme was monitored in real time during X-ray irradiation by evaluating the absorption spectral changes. Moreover, NO binding to the heme was probedviacaged NO photolysis with UV light, demonstrating the extended capability of the spectrometer for intermediate analysis.
  International Union of Crystallography (IUCr), Scientific journal
  DOI：https://doi.org/10.1107/s1600577515018275
  DOI ID：10.1107/s1600577515018275, ISSN：1600-5775
• Formation and characterization of a reactive chromium(V)-oxo complex: Mechanistic insight into hydrogen-atom transfer reactions
  Kotani, H.; Kaida, S.; Ishizuka, T.; Sakaguchi, M.; Ogura, T.; Shiota, Y.; Yoshizawa, K.; Kojima, T.
  Chemical Science, Volume：6, Number：2, 2015, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1039/c4sc02285h
  DOI ID：10.1039/c4sc02285h, ISSN：2041-6539, ORCID：178157607, SCOPUS ID：84921668381
• Radical localization in a series of symmetric Ni^II complexes with oxidized salen ligands
  Chiang, L.; Kochem, A.; Jarjayes, O.; Dunn, T.J.; Vezin, H.; Sakaguchi, M.; Ogura, T.; Orio, M.; Shimazaki, Y.; Thomas, F.; Storr, T.
  Chemistry - A European Journal, Volume：18, Number：44, 2012, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1002/chem.201201410
  DOI ID：10.1002/chem.201201410, ISSN：1521-3765, ORCID：178157617, SCOPUS ID：84961979072
• Erratum: Mechanistic insight into catalytic oxidations of organic compounds by ruthenium(IV)-oxo complexes with pyridylamine ligands (Chemical Science (2012) DOI: 10.1039/c2sc21195e)
  Ohzu, S.; Ishizuka, T.; Hirai, Y.; Jiang, H.; Sakaguchi, M.; Ogura, T.; Fukuzumi, S.; Kojima, T.
  Chemical Science, Volume：3, Number：12, First page：3541, Last page：3541, 2012, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1039/c2sc90049a
  DOI ID：10.1039/c2sc90049a, ISSN：2041-6520, ORCID：178157611, SCOPUS ID：84868095947
• Non-innocent ligand behaviour of a bimetallic Cu complex employing a bridging catecholate
  Dunn, T.J.; Chiang, L.; Ramogida, C.F.; Webb, M.I.; Savard, D.; Sakaguchi, M.; Ogura, T.; Shimazaki, Y.; Storr, T.
  Dalton Transactions, Volume：41, Number：26, 2012, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1039/c2dt30444a
  DOI ID：10.1039/c2dt30444a, ISSN：1477-9234, ORCID：178157606, SCOPUS ID：84961979053
• Photochemical activation of ruthenium(II)-pyridylamine complexes having a pyridine- N -oxide pendant toward oxygenation of organic substrates
  Kojima, T.; Nakayama, K.; Sakaguchi, M.; Ogura, T.; Ohkubo, K.; Fukuzumi, S.
  Journal of the American Chemical Society, Volume：133, Number：44, 2011, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1021/ja207572z
  DOI ID：10.1021/ja207572z, ISSN：0002-7863, ORCID：178157605, SCOPUS ID：80455129378
• A resonance Raman band assignable to the O-O stretching mode in the resting oxidized state of bovine heart cytochrome c oxidase
  Sakaguchi, M.; Shinzawa-Itoh, K.; Yoshikawa, S.; Ogura, T.
  Journal of Bioenergetics and Biomembranes, Volume：42, Number：3, 2010, [Reviewed]
  Scientific journal
  DOI：https://doi.org/10.1007/s10863-010-9282-y
  DOI ID：10.1007/s10863-010-9282-y, ISSN：0145-479X, ORCID：178157609, SCOPUS ID：77954423532

• in-cell 高感度一分子構造ダイナミクス解析システムの開発　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2025
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  Grant number：22K05018
• 共鳴ラマン分光法によるチトクロムc酸化酵素のプロトンポンプ機構の解明　　　　　　　　　　　　　　　
  2011 - 2012
  Grant amount(Total)：1300000, Direct funding：1300000
  Grant number：11J07254