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HIBINO Taku
| Natural science course | Associate Professor |
| Graduate School of Education |
Researcher information
■ Degree■ Field Of Study
■ Career
- 2008 - Present, Saitama University, Faculty of Education
- 2007 - 2008, The University of Tokyo, Graduate School of Science, Department of Biological Sciences
- 2006 - 2007, University of Toronto
- 2005 - 2006, University of Toronto
- 2004 - 2005
Performance information
■ Paper- Identification of an antibacterial polypeptide in mouse seminal vesicle secretions
Kazunori Morohoshi; Takeo Yamazaki; Keiji Kito; Ban Sato; Woojin Kang; Taku Hibino; Manabu Yoshida; Kaoru Yoshida; Teruaki Iwamoto; Mitsutoshi Yamada; Kenji Miyado; Natsuko Kawano
Journal of Reproductive Immunology, First page：103436, Last page：103436, Oct. 2021, [Reviewed]
Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.jri.2021.103436
DOI ID：10.1016/j.jri.2021.103436, ISSN：0165-0378 - Development of the coelomic cavities in larvae of the living isocrinid sea lily Metacrinus rotundus
Amemiya S; Hibino T; Minokawa T; Naruse K; Kamei Y; Uemura I; Kiyomoto M; Hisanaga S.-I; Kuraishi R
Acta Zoologica, Volume：100, Number：4, First page：414, Last page：430, Oct. 2019, [Reviewed]
Wiley, Scientific journal
DOI：https://doi.org/10.1111/azo.12274
DOI ID：10.1111/azo.12274, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103730, SCOPUS ID：85053661947 - Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility.
Miyuki Shindo; Masafumi Inui; Woojin Kang; Moe Tamano; Cai Tingwei; Shuji Takada; Taku Hibino; Manabu Yoshida; Kaoru Yoshida; Hiroshi Okada; Teruaki Iwamoto; Kenji Miyado; Natsuko Kawano
International journal of molecular sciences, Volume：20, Number：18, Sep. 2019, [Reviewed], ［International magazine］
Multiple genes, whose functions or expression are overlapping, compensate for the loss of one gene. A gene cluster in the mouse genome encodes five seminal vesicle proteins (SVS2, SVS3, SVS4, SVS5, and SVS6). These proteins are produced by male rodents and function in formation of the copulatory plug following mating. SVS2 plays an essential role in the successful internal fertilization by protecting the sperm membrane against a uterine immune attack. We hypothesized that the four remaining seminal vesicle proteins (SVPs) of this gene cluster may partially/completely compensate for the deficiency of SVS2. For confirming our hypothesis, we generated mice lacking the entire SVP-encoding gene cluster and compared their fecundity with Svs2-deficient (Svs2-/-) mice; that is, mice deficient in Svs2 alone. A single loxP site remained after the deletion of the Svs2 gene. Therefore, we inserted another loxP site by combining the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides (ssODN). Male mice lacking the entire SVP-encoding gene cluster (Svs2-6-/- mice) and thereby all five SVP proteins, generated by the deletion of 100kbp genomic DNA, showed low fecundity. However, the fecundity level was comparable with that from Svs2-/- male mice. Our results demonstrate that SVS3, SVS4, SVS5, and SVS6 do not function in the protection of sperm against a uterine immune attack in the absence of SVS2. Thus, Svs2 is the critical gene in the SVP gene cluster.
English, Scientific journal
DOI：https://doi.org/10.3390/ijms20184557
DOI ID：10.3390/ijms20184557, PubMed ID：31540031, PubMed Central ID：PMC6769479 - Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods
Hibino T; Minokawa T; Yamazaki A
Methods in Cell Biology, Volume：150, First page：81, Last page：103, Jan. 2019, [Reviewed]
DOI：https://doi.org/10.1016/bs.mcb.2018.09.012
DOI ID：10.1016/bs.mcb.2018.09.012, ORCID：57103723, SCOPUS ID：85056708456 - IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva
Katherine M. Buckley; Eric Chun Hei Ho; Taku Hibino; Catherine S. Schrankel; Nicholas W. Schuh; Guizhi Wang; Jonathan P. Rast
ELIFE, Volume：6, Number：e23481, First page：1, Last page：24, Apr. 2017, [Reviewed]
IL17 cytokines are central mediators of mammalian immunity. In vertebrates, these factors derive from diverse cellular sources. Sea urchins share a molecular heritage with chordates that includes the IL17 system. Here, we characterize the role of epithelial expression of IL17 in the larval gut-associated immune response. The purple sea urchin genome encodes 10 IL17 subfamilies (35 genes) and 2 IL17 receptors. Most of these subfamilies are conserved throughout echinoderms. Two IL17 subfamilies are sequentially strongly upregulated and attenuated in the gut epithelium in response to bacterial disturbance. IL17R1 signal perturbation results in reduced expression of several response genes including an IL17 subtype, indicating a potential feedback. A third IL17 subfamily is activated in adult immune cells indicating that expression in immune cells and epithelia is divided among families. The larva provides a tractable model to investigate the regulation and consequences of gut epithelial IL17 expression across the organism.
ELIFE SCIENCES PUBLICATIONS LTD, English, Scientific journal
DOI：https://doi.org/10.7554/eLife.23481
DOI ID：10.7554/eLife.23481, ISSN：2050-084X, ORCID：57103731, SCOPUS ID：85020441127, Web of Science ID：WOS:000402554100001 - Perturbation of gut bacteria induces a coordinated cellular immune response in the purple sea urchin larva
Eric C. H. Ho; Katherine M. Buckley; Catherine S. Schrankel; Nicholas W. Schuh; Taku Hibino; Cynthia M. Solek; Koeun Bae; Guizhi Wang; Jonathan P. Rast
IMMUNOLOGY AND CELL BIOLOGY, Volume：94, Number：9, First page：861, Last page：874, Oct. 2016, [Reviewed]
The purple sea urchin (Strongylocentrotus purpuratus) genome sequence contains a complex repertoire of genes encoding innate immune recognition proteins and homologs of important vertebrate immune regulatory factors. To characterize how this immune system is deployed within an experimentally tractable, intact animal, we investigate the immune capability of the larval stage. Sea urchin embryos and larvae are morphologically simple and transparent, providing an organism-wide model to view immune response at cellular resolution. Here we present evidence for immune function in five mesenchymal cell types based on morphology, behavior and gene expression. Two cell types are phagocytic; the others interact at sites of microbial detection or injury. We characterize immune-associated gene markers for three cell types, including a perforin-like molecule, a scavenger receptor, a complement-like thioester-containing protein and the echinoderm-specific immune response factor 185/333. We elicit larval immune responses by (1) bacterial injection into the blastocoel and (2) seawater exposure to the marine bacterium Vibrio diazotrophicus to perturb immune state in the gut. Exposure at the epithelium induces a strong response in which pigment cells (one type of immune cell) migrate from the ectoderm to interact with the gut epithelium. Bacteria that accumulate in the gut later invade the blastocoel, where they are cleared by phagocytic and granular immune cells. The complexity of this coordinated, dynamic inflammatory program within the simple larval morphology provides a system in which to characterize processes that direct both aspects of the echinoderm-specific immune response as well as those that are shared with other deuterostomes, including vertebrates.
NATURE PUBLISHING GROUP, English, Scientific journal
DOI：https://doi.org/10.1038/icb.2016.51
DOI ID：10.1038/icb.2016.51, ISSN：0818-9641, eISSN：1440-1711, ORCID：57103719, SCOPUS ID：84977068191, Web of Science ID：WOS:000386789200007 - Early stalked stages in ontogeny of the living isocrinid sea lily Metacrinus rotundus
Shonan Amemiya; Akihito Omori; Toko Tsurugaya; Taku Hibino; Masaaki Yamaguchi; Ritsu Kuraishi; Masato Kiyomoto; Takuya Minokawa
ACTA ZOOLOGICA, Volume：97, Number：1, First page：102, Last page：116, Jan. 2016, [Reviewed]
The early stalked stages of an isocrinid sea lily, Metacrinus rotundus, were examined up to the early pentacrinoid stage. Larvae induced to settle on bivalve shells and cultured in the laboratory developed into late cystideans. Three-dimensional (3D) images reconstructed from very early to middle cystideans indicated that 15 radial podia composed of five triplets form synchronously from the crescent-shaped hydrocoel. The orientation of the hydrocoel indicated that the settled postlarvae lean posteriorly. In very early cystideans, the orals, radials, basals and infrabasals, with five plates each in the crown, about five columnals in the stalk, and five terminal stem plates in the attachment disc, had already formed. In mid-cystideans, an anal plate appeared in the crown. Late cystideans cultured in the field developed into pentacrinoids about 5 months after settlement. These pentacrinoids shared many crown structures with adult sea lilies. On the other hand, many features of the stalk differed from those in adult isocrinids, while sharing many characteristics with the stalk of feather star pentacrinoids, including disc-like proximal columnals, high and slender median columnals, synarthrial articulations developmentally derived from the symplexial articulations, limited formation of cirri only in the proximal columnal(s), and an attachment disc. On the basis of these findings, phylogenetic relationships among extant crinoid orders are discussed.
WILEY-BLACKWELL, English, Scientific journal
DOI：https://doi.org/10.1111/azo.12109
DOI ID：10.1111/azo.12109, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103720, SCOPUS ID：84954392628, Web of Science ID：WOS:000368010100010 - Development ciliary bands in larvae. of the living isocrinid sea lily Metacrinus rotundus
Shonan Amemiya; Taku Hibino; Hiroaki Nakano; Masaaki Yamaguchi; Ritsu Kuraishi; Masato Kiyomoto
ACTA ZOOLOGICA, Volume：96, Number：1, First page：37, Last page：44, Jan. 2015, [Reviewed]
Embryos and larvae of an isocrinid sea lily, Metacrinus rotundus, arc described by scanning electron microscopy. Around hatching (35 h after fertilization), the outer surface of the gastrula becomes ubiquitously covered with short cilia. At 40 h, the hatched swimming embryo develops a cilia free zone of ectoderm on the ventral side. By 3 days, the very early dipleurula larva develops a cilia free zone ventrally, densely ciliated regions laterally, and a sparsely ciliated region dorsally. At this stage, the posterior and anterior ciliary bands first appear: the former runs along a low ridge separating the densely from the sparsely ciliated epidermal regions, while the latter is visible, at first discontinuously, along the boundary between the densely ciliated lateral regions and the cilia-free ventral zone. In the late dipleurula larva (5 days after fertilization), the anterior and posterior loops of ciliary bands are 1,yell defined. The transition from the dipleurula to the semidoliolaria larva occurs at 6 days as the posterior loop becomes rearranged to form incompletely circumferential ciliary bands. The larva becomes competent to settle at this stage. The arrangement of the ciliary bands on the semidoliolaria is maintained during the second week of development, 1,vhile the larva retains its competence to settle. The larval ciliary patterns described here are compared with those of stalkless crinoids and eleutherozoan echinoderms. The closest morphological similarities are between M. rotundus and the basal eleutherozoan class Asteroidea.
WILEY-BLACKWELL, English, Scientific journal
DOI：https://doi.org/10.1111/azo.12049
DOI ID：10.1111/azo.12049, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103724, PubMed ID：25641974, SCOPUS ID：84919835996, Web of Science ID：WOS:000346775600004 - Seminal vesicle protein SVS2 is required for sperm survival in the uterus
Natsuko Kawano; Naoya Araki; Kaoru Yoshida; Taku Hibino; Naoko Ohnami; Maako Makino; Seiya Kanai; Hidetoshi Hasuwa; Manabu Yoshida; Kenji Miyado; Akihiro Umezawa
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：111, Number：11, First page：4145, Last page：4150, Mar. 2014, [Reviewed]
In mammals, sperm migrate through the female reproductive tract to reach the egg; however, our understanding of this journey is highly limited. To shed light on this process, we focused on defining the functions of seminal vesicle secretion 2 (SVS2). SVS2(-/-) male mice produced sperm but were severely subfertile, and formation of a copulatory plug to cover the female genital opening did not occur. Surprisingly, even when artificial insemination was performed with silicon as a substitute for the plug, sperm fertility in the absence of SVS2 remained severely reduced because the sperm were already dead in the uterus. Thus, our results provide evidence that the uterus induces sperm cell death and that SVS2 protects sperm from uterine attack.
NATL ACAD SCIENCES, English, Scientific journal
DOI：https://doi.org/10.1073/pnas.1320715111
DOI ID：10.1073/pnas.1320715111, ISSN：0027-8424, ORCID：57103717, PubMed ID：24591616, SCOPUS ID：84896533547, Web of Science ID：WOS:000333027900061 - A novel third complement component C3 gene of Ciona intestinalis expressed in the endoderm at the early developmental stages
T. Hibino; M. Nonaka
ISJ-INVERTEBRATE SURVIVAL JOURNAL, Volume：10, Number：1, First page：29, Last page：37, Mar. 2013, [Reviewed], [Lead]
The third complement component (C3) in ascidian was reported to function as an opsonin to enhance phagocytosis and as a chemotactic factor for phagocytes, indicating that ascidian C3 works in mesodermal cavity as a humoral factor like vertebrate C3s. In the basal Eumetazoa, Cnidaria lacking mesodermal tissues, C3 was reported to work in an endodermal cavity. Evolution of structure and function of C3 is still to be clarified. Here we report the identification of the third C3 gene, CiC3-3, in the genome of an ascidian, Ciona intestinalis. Phylogenetic analysis using the entire amino acid sequences of Eumetazoan C3s indicated that CiC3-3 possess a closer relationship to vertebrate C3, C4 and C5 than other ascidian C3s. Although CiC3-3 retained the alpha-beta processing site and 6 cysteine residues in the C3a region, it lacked the intra-molecular thioester bond and the catalytic histidine residue. Instead, CiC3-3 had a unique insertion of about 70 residues long Lys/Arg-rich sequence. CiC3-3 was expressed highly in the embryonic stages, but little in the adult in contradistinction to CiC3-1 and CiC3-2. The expression of CiC3-3 in early embryonic stages was restricted to endoderm similar to cnidarian C3s. Thus, the ascidian complement system could represent a unique evolutionary stage sharing a primitive endodermal function with Cnidaria, and newly developed humoral function with vertebrates.
INVERTEBRATE SURVIVAL JOURNAL, English, Scientific journal
ISSN：1824-307X, ORCID：57103711, SCOPUS ID：84925273676, Web of Science ID：WOS:000322360500004 - Sp185/333: A novel family of genes and proteins involved in the purple sea urchin immune response
Julie Ghosh; Katherine M. Buckley; Sham V. Nair; David A. Raftos; Chase Miller; Audrey J. Majeske; Taku Hibino; Jonathan P. Rast; Mattias Roth; L. Courtney Smith
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, Volume：34, Number：3, First page：235, Last page：245, Mar. 2010, [Reviewed]
The Sp185/333 system of genes, messages and proteins are expressed in the coelomocytes of the purple sea urchin, Strongylocentrotus purpuratus, and is an extraordinary example of diversification of a putative innate immune response system in an invertebrate. Reviewed here, is the current understanding of this complex system as illustrated by sequence comparisons of the genes, messages and deduced proteins with descriptions of diversity, including preliminary results on genomic organization and descriptions of 185/333 in other echinoids. Sp185/333 gene expression in adults and embryos occurs in response to immune challenge and includes changes in the frequencies of Sp185/333-positive coelomocytes in the adults. The diversity of the Sp185/333 protein repertoire in coelomocytes is far greater than the sequence diversity encoded in the genes, which may be the result of rapid gene recombination, RNA editing and/or low-fidelity transcription, plus post-translational modifications. This review concludes with preliminary results and speculations on protein function. (C) 2009 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, English
DOI：https://doi.org/10.1016/j.dci.2009.10.008
DOI ID：10.1016/j.dci.2009.10.008, ISSN：0145-305X, ORCID：57103728, PubMed ID：19887082, SCOPUS ID：73249143320, Web of Science ID：WOS:000274832700001 - Evolution of body axes in deuterostomes viewed from left-right asymmetry in sea urchin larvae
日比野 拓
Cell technology., Volume：27, Number：6, First page：548, Last page：552, Jun. 2008, [Invited]
秀潤社, Japanese
ISSN：0287-3796, CiNii Articles ID：40016077805, CiNii Books ID：AN00069897 - Phylogenetic correspondence of the body axes in bilaterians is revealed by the right-sided expression of Pitx genes in echinoderm larvae
Taku Hibino; Atsuo Nishino; Shonan Amemiya
DEVELOPMENT GROWTH & DIFFERENTIATION, Volume：48, Number：9, First page：587, Last page：595, Dec. 2006, [Reviewed], [Lead]
Chordates and echinoderms are two of the three major deuterostome phyla and show conspicuous left-right (LR) asymmetry. The establishment of LR asymmetry has been explored in vertebrates, but is largely unknown in echinoderms. Here, we report the expression pattern of genes that are orthologous to the chordate left-side specific gene Pitx, cloned from the sea urchin Hemicentrotus pulcherrimus (HpPitx) and the starfish Asterina pectinifera (ApPitx). HpPitx transcripts were first detected bilaterally in one cell of the ventrolateral primary mesenchyme-cell aggregate of early prism larvae. New expression was detected asymmetrically in the right counterpart of a bilateral pair of mesodermal coelomic pouches and in the right ectoderm. In starfish bipinnaria larvae, the ApPitx signal was detected in the right coelomic pouch and in the right half of the ectoderm along the ciliary bands. These results suggest that the function of Pitx in establishing LR asymmetry was introduced in the last common ancestor of echinoderms and chordates. However, the right-side specific expression in echinoderm larvae is inverted compared to chordate embryos. This indicates that the LR axis is inversely represented between echinoderms and chordates, which supports the scenario that dorsoventral axis inversion was introduced into the chordate lineage by turning upside down.
BLACKWELL PUBLISHING, English, Scientific journal
DOI：https://doi.org/10.1111/j.1440-169x.2006.00892.x
DOI ID：10.1111/j.1440-169x.2006.00892.x, ISSN：0012-1592, CiNii Articles ID：10018808295, ORCID：57103733, PubMed ID：17118013, SCOPUS ID：33750892936, Web of Science ID：WOS:000241914700004 - The immune gene repertoire encoded in the purple sea urchin genome
Taku Hibino; Mariano Loza-Coll; Cynthia Messier; Audrey J. Majeske; Avis H. Cohen; David P. Terwilliger; Katherine M. Buckley; Virginia Brockton; Sham V. Nair; Kevin Berney; Sebastian D. Fugmann; Michele K. Anderson; Zeev Pancer; R. Andrew Cameron; L. Courtney Smith; Jonathan P. Rast
DEVELOPMENTAL BIOLOGY, Volume：300, Number：1, First page：349, Last page：365, Dec. 2006, [Reviewed], [Lead]
Echinoderms occupy a critical and largely unexplored phylogenetic vantage point from which to infer both the early evolution of bilaterian immunity and the underpinnings of the vertebrate adaptive immune system. Here we present an initial survey of the purple sea urchin genome for genes associated with immunity. An elaborate repertoire of potential immune receptors, regulators and effectors is present, including unprecedented expansions of innate pathogen recognition genes. These include a diverse array of 222 Toll-like receptor (TLR) genes and a coordinate expansion of directly associated signaling adaptors. Notably, a subset of sea urchin TLR genes encodes receptors with structural characteristics previously identified only in protostomes. A similarly expanded set of 203 NOD/NALP-like cytoplasmic recognition proteins is present. These genes have previously been identified only in vertebrates where they are represented in much lower numbers. Genes that mediate the alternative and lectin complement pathways are described, while gene homologues of the terminal pathway are not present. We have also identified several homologues of genes that function in jawed vertebrate adaptive immunity. The most striking of these is a gene cluster with similarity to the jawed vertebrate Recombination Activating Genes 1 and 2 (RAG1/2). Sea urchins are long-lived, complex organisms and these findings reveal an innate immune system of unprecedented complexity. Whether the presumably intense selective processes that molded these gene families also gave rise to novel immune mechanisms akin to adaptive systems remains to be seen. The genome sequence provides immediate opportunities to apply the advantages of the sea urchin model toward problems in developmental and evolutionary immunobiology. (c) 2006 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, English
DOI：https://doi.org/10.1016/j.ydbio.2006.08.065
DOI ID：10.1016/j.ydbio.2006.08.065, ISSN：0012-1606, eISSN：1095-564X, ORCID：57103721, PubMed ID：17027739, SCOPUS ID：33751549306, Web of Science ID：WOS:000242873300027 - RTK and TGF-beta signaling pathways genes in the sea urchin genome
Francois Lapraz; Eric Rottinger; Veronique Duboc; Ryan Range; Louise Duloquin; Katherine Walton; Shu-Yu Wu; Cynthia Bradham; Mariano A. Loza; Taku Hibino; Karen Wilson; Albert Poustka; Dave McClay; Lynne Angerer; Christian Gache; Thierry Lepage
DEVELOPMENTAL BIOLOGY, Volume：300, Number：1, First page：132, Last page：152, Dec. 2006, [Reviewed]
The Receptor Tyrosine kinase (RTK) and TGF-beta signaling pathways play essential roles during development in many organisms and regulate a plethora of cellular responses. From the genome sequence of Strongylocentrotus purpuratus, we have made an inventory of the genes encoding receptor tyrosine kinases and their ligands, and of the genes encoding cytokines of the TGF-beta superfamily and their downstream components.
The sea urchin genome contains at least 20 genes coding for canonical receptor tyrosine kinases. Seventeen of the nineteen vertebrate RTK families are represented in the sea urchin. Fourteen of these RTK among which ALK, CCK4/PTK7, DDR, EGFR, EPH, LMR, MET/RON, MUSK, RET, ROR, ROS, RYK, TIE and TRK are present as single copy genes while pairs of related genes are present for VEGFR, FGFR and INSR.
Similarly, nearly all the subfamilies of TGF-beta ligands identified in vertebrates are present in the sea urchin genome including the BMP, ADMP, GDF, Activin, Myostatin, Nodal and Lefty, as well as the TGF-beta sensu stricto that had not been characterized in invertebrates so far. Expression analysis indicates that the early expression of nodal, BMP2/4 and lefty is restricted to the oral ectoderm reflecting their role in providing positional information along the oral-aboral axis of the embryo. The coincidence between the emergence of TGF-beta-related factors such as Nodal and Lefty and the emergence of the deuterostome lineage strongly suggests that the ancestral function of Nodal could have been related to the secondary opening of the mouth which characterizes this clade, a hypothesis supported by functional data in the extant species.
The sea urchin genome contains 6 genes encoding TGF-beta receptors and 4 genes encoding prototypical Smad proteins. Furthermore, most of the transcriptional activators and repressors shown to interact with Smads in vertebrates have orthologues in echinoderms. Finally, the sea urchin genome contains an almost complete repertoire of genes encoding extracellular modulators of BMP signaling including Chordin, Noggin, Sclerotin, SFRP, Gremlin, DAN and Twisted gastrulation. Taken together, these findings indicate that the sea urchin complement of genes of the RTK and TGF-beta signaling pathways is qualitatively very similar to the repertoire present in vertebrates, and that these genes are part of the common genetool kit for intercellular signaling of deuterostomes. (c) 2006 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, English
DOI：https://doi.org/10.1016/j.ydbio.2006.08.048
DOI ID：10.1016/j.ydbio.2006.08.048, ISSN：0012-1606, eISSN：1095-564X, CiNii Articles ID：80018716920, ORCID：57103716, PubMed ID：17084834, SCOPUS ID：33846808910, Web of Science ID：WOS:000242873300013 - Research article - The genome of the sea urchin Strongylocentrotus purpuratus
Erica Sodergren; George M. Weinstock; Eric H. Davidson; R. Andrew Cameron; Richard A. Gibbs; George M. Weinstock; Robert C. Angerer; Lynne M. Angerer; Maria Ina Arnone; David R. Burgess; Robert D. Burke; R. Andrew Cameron; James A. Coffman; Eric H. Davidson; Michael Dean; Maurice R. Elphick; Charles A. Ettensohn; Kathy R. Foltz; Amro Hamdoun; Richard O. Hynes; William H. Klein; William Marzluff; David R. McClay; Robert L. Morris; Arcady Mushegian; Jonathan P. Rast; Erica Sodergren; L. Courtney Smith; Michael C. Thorndyke; Victor D. Vacquier; George M. Weinstock; Gary M. Wessel; Greg Wray; Lan Zhang; Erica Sodergren; George M. Weinstock; Robert C. Angerer; Lynne M. Angerer; R. Andrew Cameron; Eric H. Davidson; Christine G. Elsik; Olga Ermolaeva; Wratko Hlavina; Gretchen Hofmann; Paul Kitts; Melissa J. Landrum; Aaron J. Mackey; Donna Maglott; Georgia Panopoulou; Albert J. Poustka; Kim Pruitt; Victor Sapojnikov; Xingzhi Song; Alexandre Souvorov; Victor Solovyev; Zheng Wei; Charles A. Whittaker; Kim Worley; Lan Zhang; Erica Sodergren; George M. Weinstock; K. James Durbin; Richard A. Gibbs; Yufeng Shen; Xingzhi Song; Kim Worley; Lan Zhang; Greg Wray; Olivier Fedrigo; David Garfield; Ralph Haygood; Alexander Primus; Rahul Satija; Tonya Severson; Lan Zhang; Erica Sodergren; George M. Weinstock; Manuel L. Gonzalez-Garay; Andrew R. Jackson; Aleksandar Milosavljevic; Xingzhi Song; Mark Tong; Kim Worley; Charles A. Ettensohn; R. Andrew Cameron; Christopher E. Killian; Melissa J. Landrum; Brian T. Livingston; Fred H. Wilt; James A. Coffman; William Marzluff; Arcady Mushegian; Nikki Adams; Robert Belle; Seth Carbonneau; Rocky Cheung; Patrick Cormier; Bertrand Cosson; Jenifer Croce; Antonio Fernandez-Guerra; Anne-Marie Geneviere; Manisha Goel; Hemant Kelkar; Julia Morales; Odile Mulner-Lorillon; Anthony J. Robertson; Amro Hamdoun; Jared V. Goldstone; Nikki Adams; Bryan Cole; Michael Dean; David Epel; Bert Gold; Mark E. Hahn; Meredith Howard-Ashby; Mark Scally; John J. Stegeman; Robert L. Morris; Erin L. Allgood; Jonah Cool; Kyle M. Judkins; Shawn S. McCafferty; Ashlan M. Musante; Robert A. Obar; Amanda P. Rawson; Blair J. Rossetti; David R. Burgess; Erin L. Allgood; Jonah Cool; Ian R. Gibbons; Matthew P. Hoffman; Kyle M. Judkins; Andrew Leone; Shawn S. McCafferty; Robert L. Morris; Ashlan M. Musante; Robert A. Obar; Amanda P. Rawson; Blair J. Rossetti; Gary M. Wessel; Eric H. Davidson; R. Andrew Cameron; Sorin Istrail; Stefan C. Materna; Manoj P. Samanta; Viktor Stolc; Waraporn Tongprasit; Qiang Tu; Robert C. Angerer; Lynne M. Angerer; Zheng Wei; Richard O. Hynes; Karl-Frederik Bergeron; Bruce P. Brandhorst; Robert D. Burke; Charles A. Whittaker; James Whittle; R. Andrew Cameron; Kevin Berney; David J. Bottjer; Cristina Calestani; Eric H. Davidson; Kevin Peterson; Elly Chow; Qiu Autumn Yuan; Eran Elhaik; Christine G. Elsik; Dan Graur; Justin T. Reese; Ian Bosdet; Shin Heesun; Marco A. Marra; Jacqueline Schein; Michael Dean; Amro Hamdoun; Jonathan P. Rast; L. Courtney Smith; Michele K. Anderson; Kevin Berney; Virginia Brockton; Katherine M. Buckley; R. Andrew Cameron; Avis H. Cohen; Sebastian D. Fugmann; Taku Hibino; Mariano Loza-Coll; Audrey J. Majeske; Cynthia Messier; Sham V. Nair; Zeev Pancer; David P. Terwilliger; Robert D. Burke; Maurice R. Elphick; William H. Klein; Michael C. Thorndyke; Cavit Agca; Lynne M. Angerer; Enrique Arboleda; Maria Ina Arnone; Bruce P. Brandhorst; Nansheng Chen; Allison M. Churcher; F. Hallboeoek; Glen W. Humphrey; Richard O. Hynes; Mohammed M. Idris; Takae Kiyama; Shuguang Liang; Dan Mellott; Xiuqian Mu; Greg Murray; Robert P. Olinski; Florian Raible; Matthew Rowe; John S. Taylor; Kristin Tessmar-Raible; D. Wang; Karen H. Wilson; Shunsuke Yaguchi; Kathy R. Foltz; Victor D. Vacquier; Gary M. Wessel; Terry Gaasterland; Blanca E. Galindo; Herath J. Gunaratne; Meredith Howard-Ashby; Glen W. Humphrey; Celina Juliano; Masashi Kinukawa; Gary W. Moy; Anna T. Neill; Mamoru Nomura; Michael Raisch; Anna Reade; Michelle M. Roux; Jia L. Song; Yi-Hsien Su; Ian K. Townley; Ekaterina Voronina; Julian L. Wong; Maria Ina Arnone; Michael C. Thorndyke; Gabriele Amore; Lynne M. Angerer; Enrique Arboleda; Margherita Branno; Euan R. Brown; Vincenzo Cavalieri; Veronique Duboc; Louise Duloquin; Maurice R. Elphick; Constantin Flytzanis; Christian Gache; Anne-Marie Geneviere; Mohammed M. Idris; Francois Lapraz; Thierry Lepage; Annamaria Locascio; Pedro Martinez; Giorgio Matassi; Valeria Matranga; David R. McClay; Julia Morales; Albert J. Poustka; Florian Raible; Ryan Range; Francesca Rizzo; Eric Roettinger; Matthew Rowe; Kristin Tessmar-Raible; Erica Sodergren; George M. Weinstock; Karen Wilson; David R. McClay; Lynne M. Angerer; Maria Ina Arnone; Wendy Beane; Cynthia Bradham; Christine Byrum; Jenifer Croce; Veronique Duboc; Louise Duloquin; Christian Gache; Anne-Marie Geneviere; Tom Glenn; Taku Hibino; Sofia Hussain; Francois Lapraz; Thierry Lepage; Brian T. Livingston; Mariano Loza; Gerard Manning; Esther Miranda; Ryan Range; Francesca Rizzo; Eric Roettinger; Rebecca Thomason; Katherine Walton; Zheng Wei; Gary M. Wessel; Athula Wikramanayke; Karen H. Wilson; Charles Whittaker; Shu-Yu Wu; Ronghui Xu; Eric H. Davidson; Maria Ina Arnone; Margherita Branno; C. Titus Brown; R. Andrew Cameron; Lili Chen; Rachel F. Gray; Meredith Howard-Ashby; Sorin Istrail; Pei Yun Lee; Annamaria Locascio; Pedro Martinez; Stefan C. Materna; Jongmin Nam; Paola Oliveri; Francesca Rizzo; Joel Smith; Donna Muzny; Erica Sodergren; Richard A. Gibbs; George M. Weinstock; Stephanie Bell; Joseph Chacko; Andrew Cree; Stacey Curry; Clay Davis; Huyen Dinh; Shannon Dugan-Rocha; Jerry Fowler; Rachel Gill; Cerrissa Hamilton; Judith Hernandez; Sandra Hines; Jennifer Hume; LaRonda Jackson; Angela Jolivet; Christie Kovar; Sandra Lee; Lora Lewis; George Miner; Margaret Morgan; Lynne V. Nazareth; Geoffrey Okwuonu; David Parker; Ling-Ling Pu; Yufeng Shen; Rachel Thom; Rita Wright
SCIENCE, Volume：314, Number：5801, First page：941, Last page：952, Nov. 2006, [Reviewed]
AMER ASSOC ADVANCEMENT SCIENCE, English, Scientific journal
DOI：https://doi.org/10.1126/science.1133609
DOI ID：10.1126/science.1133609, ISSN：0036-8075, CiNii Articles ID：30020565990, ORCID：57103713, PubMed ID：17095691, SCOPUS ID：33750995860, Web of Science ID：WOS:000241896000039 - Review - Genomic insights into the immune system of the sea urchin
Jonathan P. Rast; L. Courtney Smith; Mariano Loza-Coll; Taku Hibino; Gary W. Litman
SCIENCE, Volume：314, Number：5801, First page：952, Last page：956, Nov. 2006, [Reviewed]
Comparative analysis of the sea urchin genome has broad implications for the primitive state of deuterostome host defense and the genetic underpinnings of immunity in vertebrates. The sea urchin has an unprecedented complexity of innate immune recognition receptors relative to other animal species yet characterized. These receptor genes include a vast repertoire of 222 Toll-like receptors, a superfamily of more than 200 NACHT domain - leucine- rich repeat proteins ( similar to nucleotide-binding and oligomerization domain ( NOD) and NALP proteins of vertebrates), and a large family of scavenger receptor cysteine-rich proteins. More typical numbers of genes encode other immune recognition factors. Homologs of important immune and hematopoietic regulators, many of which have previously been identified only from chordates, as well as genes that are critical in adaptive immunity of jawed vertebrates, also are present. The findings serve to underscore the dynamic utilization of receptors and the complexity of immune recognition that may be basal for deuterostomes and predicts features of the ancestral bilaterian form.
AMER ASSOC ADVANCEMENT SCIENCE, English
DOI：https://doi.org/10.1126/science.1134301
DOI ID：10.1126/science.1134301, ISSN：0036-8075, CiNii Articles ID：80018776789, ORCID：57103725, PubMed ID：17095692, SCOPUS ID：33750980112, Web of Science ID：WOS:000241896000040 - Ion flow regulates left-right asymmetry in sea urchin development
T Hibino; Y Ishii; M Levin; A Nishino
DEVELOPMENT GENES AND EVOLUTION, Volume：216, Number：5, First page：265, Last page：276, May 2006, [Reviewed], [Lead]
The degree of conservation among phyla of early mechanisms that pattern the left - right (LR) axis is poorly understood. Larvae of sea urchins exhibit consistently oriented LR asymmetry. The main part of the adult rudiment is formed from the left coelomic sac of larvae, the left hydrocoel. Although this left preference is conserved among all echinoderm larvae, its mechanism is largely not understood. Using two marker genes, HpNot and HpFoxFQ-like, which are asymmetrically expressed during larval development of the sea urchin Hemicentrotus pulcherrimus, we examined in this study the possibility that the recently discovered ion flux mechanism controls asymmetry in sea urchins as it does in several vertebrate species. Several ion-transporter inhibitors were screened for the ability to alter the expression of the asymmetric marker genes. Blockers of the H+/K+-ATPase ( omeprazole, lansoprazole and SCH28080), as well as a calcium ionophore (A23187), significantly altered the normal sidedness of asymmetric gene expression. Exposure to omeprazole disrupted the consistent asymmetry of adult rudiment formation in larvae. Immuno-detection revealed that H+/K+-ATPase-like antigens in sea urchin embryos were present through blastula stage and exhibited a striking asymmetry being present in a single blastomere in 32-cell embryos. These results suggest that, as in vertebrates, endogenous spatially-regulated early transport of H+ and/or K+, and also of Ca2+, functions in the establishment of LR asymmetry in sea urchin development.
SPRINGER, English, Scientific journal
DOI：https://doi.org/10.1007/s00427-005-0051-6
DOI ID：10.1007/s00427-005-0051-6, ISSN：0949-944X, CiNii Articles ID：10024443340, ORCID：57103722, PubMed ID：16534626, SCOPUS ID：33645870420, Web of Science ID：WOS:000236967500004 - Molecular heterotopy in the expression of Brachyury orthologs in order Clypeasteroida (irregular sea urchins) and order Echinoida (regular sea urchins)
T Hibino; Y Harada; T Minokawa; M Nonaka; S Amemiya
DEVELOPMENT GENES AND EVOLUTION, Volume：214, Number：11, First page：546, Last page：558, Nov. 2004, [Reviewed], [Lead]
The expression patterns of Brachyury (Bra) orthologs in the development of four species of sand dollars (order: Clypeasteroida), including a direct-developing species, and of a sea urchin species (order: Echinoida) were investigated during the period from blastula to the pluteus stage, with special attention paid to the relationship between the expression pattern and the mode of gastrulation. The sand dollar species shared two expression domains of the Bra orthologs with the Echinoida species, in the vegetal ring (the first domain) and the oral ectoderm (the second domain). The following heterotopic changes in the expression of the Bra genes were found among the sand dollar species and between the sand dollars and the Echinoida species. (1) The vegetal ring expressing Bra in the sand dollars was much wider and was located at a higher position along the AV axis, compared with that in the Echinoida species. The characteristic Bra expression in the vegetal ring of the sand dollar embryos was thought to be involved in the mode of gastrulation, in which involution continues from the beginning of invagination until the end of gastrulation. (2) Two of the three indirect-developing sand dollar species that were examined exhibited a third domain, in which Bra was expressed on the oral side of the archenteron. (3) In the direct-developing sand dollar embryos, Bra was expressed with an oral-aboral asymmetry in the vegetal ring and with a left-right asymmetry in the oral ectoderm. In the Echinoida species, Bra was expressed in the vestibule at the six-armed pluteus stage.
SPRINGER, English, Scientific journal
DOI：https://doi.org/10.1007/s00427-004-0437-x
DOI ID：10.1007/s00427-004-0437-x, ISSN：0949-944X, ORCID：57103715, SCOPUS ID：8544284103, Web of Science ID：WOS:000224954800003 - Regrowth of the stalk of the sea lily, Metacrinus rotundus (Echinodermata : crinoidea)
H Nakano; T Hibino; Y Hara; T Oji; S Amemiya
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-COMPARATIVE EXPERIMENTAL BIOLOGY, Volume：301A, Number：6, First page：464, Last page：471, Jun. 2004, [Reviewed]
Sea lilies are critical to understanding the evolution of the echinoderm body plan, because they are the only extant group whose adults possess a stalk, a prevalent feature in the radiation of a number of primitive echinoderm lineages. Extensive crown regeneration ability has been reported in Metacrinus rotundus, but the regenerative potential of the stalk has never been determined in any species of sea lilies. In this study, we show that M. rotundus whose stalks have been completely excised are capable of stalk regeneration. The process is similar to the growth of the original stalk, but much slower, and the regenerated stalks are not morphologically identical to the original stalk. Since stalk regeneration, in contrast to well-studied regeneration events, probably requires little additional activation of morphogenetic programs, we refer to the stalk regeneration phenomenon as "stalk regrowth" to distinguish it as a special form of regeneration. Since specimens whose entire stalk below the basal plates had been removed were able to regrow, the basal plates, and probably the aboral nerve center within them, are essential For stalk regrowth. Sea lily stalk regrowth is described in detail, and the evolution of feather stars is discussed in light of the growth pattern of the sea lily stalk. (C) 2004 Wiley-Liss, Inc.
WILEY-LISS, English, Scientific journal
DOI：https://doi.org/10.1002/jez.a.77
DOI ID：10.1002/jez.a.77, ISSN：0022-104X, ORCID：57103729, SCOPUS ID：3042850763, Web of Science ID：WOS:000221985500002 - Development of the sea lily Metacrinus rotundus: Comparisons with feather stars
Nakano H; Hibino T; Hara Y; Oji T; Amemiya S
Proceedings of the 11th International Echinoderm Conference, First page：41-44, Jan. 2004, [Reviewed]
English, International conference proceedings - Larval stages of a living sea lily (stalked crinoid echinoderm)
H Nakano; T Hibino; T Oji; Y Hara; S Amemiya
NATURE, Volume：421, Number：6919, First page：158, Last page：160, Jan. 2003, [Reviewed]
The embryos and larvae of stalked crinoids, which are considered the most basal group of extant echinoderms(1,2), have not previously been described. In contrast, much is known about the development of the more accessible stalkless crinoids (feather stars)(3), which are phylogenetically derived from stalked forms(4). Here we describe the development of a sea lily from fertilization to larval settlement. There are two successive larval stages: the first is a non-feeding auricularia stage with partly longitudinal ciliary bands (similar to the auricularia and bipinnaria larvae of holothurian and asteroid echinoderms, respectively); the second is a doliolaria larva with circumferential ciliary bands (similar to the earliest larval stage of stalkless crinoids). We suggest that a dipleurula-type larva is primitive for echinoderms and is the starting point for the evolution of additional larval forms within the phylum. From a wider evolutionary viewpoint, the demonstration that the most basal kind of echinoderm larva is a dipleurula is consistent with Garstang's auricularia theory(5) for the phylogenetic origin of the chordate neural tube.
NATURE PUBLISHING GROUP, English, Scientific journal
DOI：https://doi.org/10.1038/nature01236
DOI ID：10.1038/nature01236, ISSN：0028-0836, CiNii Articles ID：80015736591, ORCID：57103726, PubMed ID：12520300, SCOPUS ID：0346634891, Web of Science ID：WOS:000180267200038 - The behavior and the morphology of sea lilies with shortened stalks: Implications on the evolution of feather stars
H Nakano; T Hibino; Y Hara; T Oji; S Amemiya
ZOOLOGICAL SCIENCE, Volume：19, Number：8, First page：961, Last page：964, Aug. 2002, [Reviewed]
Extant crinoids can be divided into two groups, stalked sea lilies and stalkless feather stars. Feather stars are considered to have evolved from stalked ancestors by losing most of the stalk, but other differences are present between the two groups. The unsegmented centrodorsal, long and curved cirri near the crown, small calyx, and the ability to swim are all feather star features not found in the sea lilies. To figure out which of the above features evolved directly correlating with loss of the stalk in feather stars, we cut off the stalk from the sea lily Metacrinus rotundus and kept them alive in an aquarium. The specimens with shortened stalks were able to stand and crawl with their arms without the support of their stalks, but swimming was not observed for any of the animals. Morphologically, neither fusion of the remaining segments nor the reduction of the size of the calyx were observed, but the cirri became long and curved near the crown. Therefore, the extant sea lilies possess a potential to adapt to incidents of stalk loss. Specimens autotomizing most of their stalks were observed, suggesting that the potential is actually employed in nature. This mechanism linking the reduction of the stalk and the changes in the morphology of cirri may have played an important role in the evolution of the feather stars, if the stalked ancestors of feather stars also possessed this potential. Experimental zoological approaches as this study may provide new insights to the questions of evolution.
ZOOLOGICAL SOC JAPAN, English, Scientific journal
DOI：https://doi.org/10.2108/zsj.19.961
DOI ID：10.2108/zsj.19.961, ISSN：0289-0003, CiNii Articles ID：110003372800, ORCID：57103714, PubMed ID：12193811, SCOPUS ID：0036703130, Web of Science ID：WOS:000179250500014 - The centrosome-attracting body, microtubule system, and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo
T Nishikata; T Hibino; H Nishida
DEVELOPMENTAL BIOLOGY, Volume：209, Number：1, First page：72, Last page：85, May 1999, [Reviewed]
Many kinds of animal embryos exhibit stereotyped cleavage patterns during early embryogenesis. In the ascidian Halocynthia roretzi, cleavage patterns are invariant but they are complicated by successive unequal cleavages that occur in the posterior region. Here we, report the essential roles of a novel structure, called the centrosome-attracting body (CAB), which exists in the posterior pole cortex of cleaving embryos, in generating unequal cleavages. By removing and transplanting posterior egg cytoplasm and by treatment with sodium dodecyl sulfate, we demonstrated that loss of the CAB resulted in abolishment of unequal cleavage, while ectopic formation of the CAB caused ectopic unequal cleavages to occur. Experiments with a microtubule inhibitor demonstrated that the centrosome and nucleus were attracted toward the posterior cortex, where the CAB is located, by shortening of microtubule bundles formed between the centrosome and the CAB. Consequently, the mitotic apparatus was positioned asymmetrically, resulting in unequal cleavage. Immunohistochemistry provided evidence that a microtubule motor protein, a kinesin or kinesin-like molecule, may be associated with the CAB. formation of the CAB during the early cleavage stage was resistant to treatment with the microtubule inhibitor. In contrast, the integrity of the CAB was lost upon treatment with a microfilament inhibitor. We propose that the CAB plays key roles in the orientation and positioning of cleavage planes during unequal cell division. (C) 1999 Academic Press.
ACADEMIC PRESS INC, English, Scientific journal
DOI：https://doi.org/10.1006/dbio.1999.9244
DOI ID：10.1006/dbio.1999.9244, ISSN：0012-1606, CiNii Articles ID：80011048021, ORCID：57103727, PubMed ID：10208744, SCOPUS ID：0033135884, Web of Science ID：WOS:000079924400007 - Centrosome-attracting body: A novel structure closely related to unequal cleavages in the ascidian embryo
T Hibino; T Nishikata; H Nishida
DEVELOPMENT GROWTH & DIFFERENTIATION, Volume：40, Number：1, First page：85, Last page：95, Feb. 1998, [Reviewed]
The mechanism of unequal cleavage is one of the most intriguing subjects in cell biology. Previous studies of unequal cleavage have focused on a limited number of organisms such as yeasts, nematodes, sea urchins and annelids. The cleavage pattern of the ascidian embryo is invariant. In the ascidian embryo, the posterior-most blastomeres divide unequally in three successive cleavages. In the present study, it was shown that the ascidian embryo provides another good experimental system with which to analyze the mechanism of unequal cleavage. A novel structure, designated as CAB (centrosome-attracting body), which was found specifically in the unequally cleaving blastomeres was described. In the course of unequal cleavages, first, a thick microtubule bundle appeared between CAB and one of the centrosomes. Then with the shortening of the microtubule bundle, the nucleus with the centrosome was drawn toward CAB, situated at the posterior cortex of the blastomere. Finally, a cleavage furrow formed in the middle of the asymmetrically located mitotic apparatus and produced two blastomeres of different size, generating a smaller cell that inherits CAB. The CAB seemed to play an essential role in the unequal cleavages in the ascidian embryo.
BLACKWELL PUBLISHING ASIA, English, Scientific journal
DOI：https://doi.org/10.1046/j.1440-169X.1998.t01-5-00010.x
DOI ID：10.1046/j.1440-169X.1998.t01-5-00010.x, ISSN：0012-1592, ORCID：57103732, SCOPUS ID：0031943947, Web of Science ID：WOS:000072500000010
- Aquatic Fauna of Hozoji Pond Surveyed Using Environmental DNA Analyses : Compared to Field Surveys
吉田 竜矢; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, Volume：68, Number：1, First page：317, Last page：331, 2019
Faculty of Education, Saitama University, Japanese
ISSN：1881-5146, CiNii Articles ID：120006585307 - Four Years of Change in Aquatic Fauna in Hozoji Pond After the Emergency Research for Saving the Endangered Aquatic Plant, Aldrovanda vesiculosa L.
吉田 竜矢; 宇田川 貴大; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, Volume：67, Number：2, First page：341, Last page：351, 2018
Faculty of Education, Saitama University, Japanese
ISSN：1881-5146, CiNii Articles ID：120006533500 - 私の実験動物＃５ タコノマクラ
日比野 拓
Volume：49, First page：20, Last page：23, 2018 - Development of immune card games for teaching materials
HIBINO Taku
JADCI News, Volume：46, First page：5, Last page：6, Jun. 2017
Japanese - Developing an Environmental Education Program Closely Related to a Local Community : Presenting Data on Aquatic Fauna in Hozoji Pond, a Natural Habitat of Aldrovanda vesiculosa L.
吉田 竜矢; 田端 雄樹; 伊藤 悠昭; 山本 孔紀; 矢辺 徹; 金子 康子; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, Volume：66, Number：2, First page：609, Last page：622, 2017
Faculty of Education, Saitama University, Japanese
ISSN：1881-5146, CiNii Articles ID：120006389474 - TLR Card Game : Developed for Understanding Pathogen Recognition by the Innate Immune System
日比野 拓
埼玉大学紀要. 教育学部, Volume：65, Number：2, First page：261, Last page：270, Oct. 2016
Toll-like receptors (TLRs) are protein sensors located on immune cells, which function as pathogen recognition by the innate immune system. These TLRs form a homodimer or a heterodimer, which recognizes distinct pathogen-associated molecular patterns. The explanation of the TLRs and their function have been introduced on Japanese biological textbooks for high school students since 2012. Here, I developed a card game, TLR Card Game, as an educational material for understanding the mechanism of the innate pathogen recognition. The TLR Card Game is for multiplayers who are surrounding a table where several pairs of a phagocytic cell board and a pathogen card are set. Each player has to present two TLR cards from his own hand in his turn, making a TLR dimer corresponding to a pathogen card presenting on the table. When a TLR card-pair is presented correctly, a player can get several points, and the player with highest points finally gets win. The TLR Card Game was carried out in an extracurricular class of a high school in Saitama City. The effectiveness of the game was assessed by open- and close-ended questionnaires for 30 students. All the students got interested in the TLR Card Game and its immunology. Some students, however, answered that the game and the immune mechanism were not much linked in their thought. The TLR Card Game is considered useful for students attracted towards immunology, but the instructions before use needed to be improved.
埼玉大学教育学部, Japanese
ISSN：1881-5146, CiNii Articles ID：120005853513 - 実験・観察の活動を取り入れた生活科授業―教科内容学模索の取り組み―
鶴ヶ谷柊子; 日比野拓
埼玉大学教育学部附属教育実践総合センター紀要, Number：15, First page：135‐140, Last page：140, 26 Feb. 2016
近年、教科専門と教科教育を架橋する分野として「教科内容学」の研究が進んでいる。小学校低学年の教科である生活科は「具体的な活動や体験」を通した学びが重視されている。本研究では、教科内容学の視点から、生活科の講義における、実験・観察の活動の導入と学生の生活科に対する理解の関連について実践と検討を行った。その結果、具体的な活動を講義に取り入れることは有効であるが、受講者の持つ知識に応じて、実験・観察の活動内容を組み立てることが、生活科の特徴への理解を深める上でも必要であることがわかった。
Japanese
ISSN：1347-7420, J-Global ID：201702290329001002, CiNii Articles ID：120005818438, CiNii Books ID：AA11948173 - ムジナモ保全のための遺伝的多様性の解析
日比野 拓; 川合 真紀
First page：72, Last page：81, 2015
Japanese - 水生動物相の変遷とムジナモ食害の調査
日比野 拓; 宇田川 貴大; 伊藤 悠昭; 矢辺 徹; 梅沢 一弘; 関森 清己
First page：82, Last page：96, 2015
Japanese - ウニ幼生における貪食作用に関わる細胞の同定
日比野 拓
Volume：7, 2009
Japanese - ウニ幼生の左右非対称性から後口動物の体軸の進化を考える
日比野 拓
Volume：21, First page：1, Last page：4, 2009
Japanese, Introduction other - CHANGES IN THE MORPHOLOGY OF SEA LILIES WITH SHORTENED STALKS(Taxonomy and Systematics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :
Nakano Hiroaki; Hibino Taku; Hara Yuko; Oji Tatsuo; Amemiya Shonan
Zoological science, Volume：19, Number：12, First page：1425, Last page：1425, 2002
Zoological Society of Japan, English
ISSN：0289-0003, CiNii Articles ID：110003376235, CiNii Books ID：AA10545874 - DEVELOPMENT OF THE STALKED CRINOID, METACRINUS ROTUNDUS(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :
Nakano Hiroaki; Hara Yuko; Oji Tatsuo; Amemiya Shonan; Hibino Taku
Zoological science, Volume：18, First page：79, Last page：79, 2001
Zoological Society of Japan, English
ISSN：0289-0003, CiNii Articles ID：110003372443, CiNii Books ID：AA10545874
- ぼくらは「生物学」のおかげで生きている (素晴らしきサイエンス)
金子康子; 日比野拓
Dec. 2015
Total pages：232
ISBN：4788911701, ASIN：4788911701, EAN：9784788911703
■ Research projects
- 科学と人間生活との関わりを理解し生きる力を育む教材開発
01 Apr. 2021 - 31 Mar. 2024
Grant amount(Total)：3380000, Direct funding：2600000, Indirect funding：780000
Grant number：21K02898 - Study on the post-embryonic development of the echinoids
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2018 - 31 Mar. 2021
Minokawa Takuya, Tohoku University
Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
The present study deals the questions related to post-embryonic development, such as what is a larva, what is an adult, and what is the mechanism that forms an adult. We focus on the following two topics: [1] Formation process and structure of adult rudiment in normal echinoid development, and [2] Understanding the mechanical relationship between embryonic and post-embryonic development using the regeneration experimental system. For [1], We have discovered a new feature in the morphological change of the digestive tract during the metamorphosis period from larva to adult. Regarding [2], it was verified whether or not the larval constituent cells are involved in post-embryonic development using a larval regeneration experimental system. It was confirmed that the larvae can regenerate the missing part and reconstruct the functionally and structurally normal digestive tract even if most of the digestive tract is removed experimentally.
Grant number：18K06307 - 免疫システムと社会をつなぐ教材の開発と普及
Apr. 2017 - Mar. 2020
Principal investigator
Competitive research funding - Development of educational materials to assist students with understanding cellular and molecular immunity
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Apr. 2014 - Mar. 2017
HIBINO Taku, Saitama University, Principal investigator
Grant amount(Total)：4810000, Direct funding：3700000, Indirect funding：1110000
Toll-like receptors (TLRs) are protein sensors located on immune cells, which function as pathogen recognition by the innate immune system. I developed TLR Card Game as an educational material to assist students with understanding the mechanism of the innate pathogen recognition. The TLR Card Game was carried out in an extracurricular class of a high school in Saitama City. The effectiveness of the material was assessed by questionnaires for 30 students. All the students got interested in the game and its immunology. Next, I developed An Adventure to Comprehend Immunity, which is a board game for connecting a desktop learning of immunology with an everyday occurrence and the world. Finally, the embryos of three echinoids were tested if they are suitable to observe in vivo phagocytosis. A swift phagocytosis was occurred in the blastocoel of the embryo of Clypeaster japonicus, thus this species is better for educational experiment.
Competitive research funding, Grant number：26350225 - Molecular evolution of ancient TIR containing gene family in innate immunity
JSPS, Grants-in-Aid for Scientific Research(若手研究(B)), 若手研究(B), Apr. 2010 - Mar. 2012
Taku HIBINO, 埼玉大学, Principal investigator
Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
To unveil a repertoire of Toll-like receptors (TLRs) and TIR containing signaling molecules in a basal deuterostome, we analyzed EST of a stalked crinoid, Metacrinus rotundas, whose embryonic and larval cDNA have been sequenced by Next Gene Sequencing. So far, we identified 6 TLRs that consist of 3 genes of fly-type and 3 genes of vertebrate type TLRs. MyD88-like genes and Sarm-like genes were also identified in the cDNA of the sea lily, suggesting these genes exist in a basal deuterostome. We found unique TIR containing gene families, CARD-TIR and TPR-TIR, in the sequencing data of the sea lily, which have not been in the genome of the sea urchin, but in that of amphioxus. These data suggest a basal deuterostome could utilize multigenes of TLRs and various types of TIR containing genes. And the refinement of TLRs and signaling molecules could happen after the appearance of acquired immunity.
Competitive research funding, Grant number：22770232 - A project which gives surprise and impression and makes science favorite in order to prevent dislike of science.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 2009 - 2012
ASHIDA Minoru; OHMUKAI Ryuzo; HIBINO Taku; OKAMOTO Kazuaki; SHIMIZU Makoto; KATAHIRA Katsuhiro; YATSUKA Mitsunori; ASHIDA Masami, Saitama University
Grant amount(Total)：19240000, Direct funding：14800000, Indirect funding：4440000
Various enterprises were undertaken in order to prevent dislike of science. The students were dispatched to elementary schools as a helper. They were engaged in arrangement of a science laboratory and assistance of a lesson. The students were dispatched to the event of a science museum, the studying classroom in Misato, the contact enterprise of Kawagoe, etc. In the observation experiment classroom, we gave children various surprise and impression at elementary schools, etc. Moreover, astronomical observation meetings and workshops were held. We replied to many questions by the homepage and exhibited how to make solutions, simulation of experiments and science calendars.
Grant number：21300288 - Molecular evolution of multiplicity of Toll-like receptors in echinoderm genome
JSPS, Grants-in-Aid for Scientific Research(若手研究(スタートアップ)), 若手研究(スタートアップ), Apr. 2008 - Mar. 2010
Taku HIBINO, 埼玉大学, Principal investigator
Grant amount(Total)：3302000, Direct funding：2540000, Indirect funding：762000
It has been known that approximately 10 Toll-like receptors (TLRs) in the genome of fruit fly and human. On the other hand the genomes of marine invertebrates such as a sea urchin, an amphioxus and a polychaete contain approximately 70 to 200 TLRs forming multigene family. We investigated molecular evolution of TLRs and innate immune related genes between human and fruit fly, and marine invertebrates. Multiplication of IL-17 and Sarm-like gene family that contains an old-TIR domain were found uniquely in these marine invertebrates. Appearance of these gene families may be related to multiplicity of TLRs.
Competitive research funding, Grant number：20870007 - 免疫細胞分化の遺伝子制御ネットワーク解析
Apr. 2005 - Mar. 2006
Principal investigator
Competitive research funding - -
Competitive research funding