SEARCH
検索詳細
日比野 拓（ヒビノ タク）
| 教育学部 学校教育教員養成課程 自然科学講座 | 准教授 |
| 教育学研究科 |
業績情報
■ 論文- Identification of an antibacterial polypeptide in mouse seminal vesicle secretions
Kazunori Morohoshi; Takeo Yamazaki; Keiji Kito; Ban Sato; Woojin Kang; Taku Hibino; Manabu Yoshida; Kaoru Yoshida; Teruaki Iwamoto; Mitsutoshi Yamada; Kenji Miyado; Natsuko Kawano
Journal of Reproductive Immunology, 開始ページ：103436, 終了ページ：103436, 2021年10月, ［査読有り］
Elsevier BV, 研究論文（学術雑誌）
DOI：https://doi.org/10.1016/j.jri.2021.103436
DOI ID：10.1016/j.jri.2021.103436, ISSN：0165-0378 - Development of the coelomic cavities in larvae of the living isocrinid sea lily Metacrinus rotundus
Amemiya S; Hibino T; Minokawa T; Naruse K; Kamei Y; Uemura I; Kiyomoto M; Hisanaga S.-I; Kuraishi R
Acta Zoologica, 巻：100, 号：4, 開始ページ：414, 終了ページ：430, 2019年10月, ［査読有り］
Wiley, 研究論文（学術雑誌）
DOI：https://doi.org/10.1111/azo.12274
DOI ID：10.1111/azo.12274, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103730, SCOPUS ID：85053661947 - Deletion of a Seminal Gene Cluster Reinforces a Crucial Role of SVS2 in Male Fertility.
Miyuki Shindo; Masafumi Inui; Woojin Kang; Moe Tamano; Cai Tingwei; Shuji Takada; Taku Hibino; Manabu Yoshida; Kaoru Yoshida; Hiroshi Okada; Teruaki Iwamoto; Kenji Miyado; Natsuko Kawano
International journal of molecular sciences, 巻：20, 号：18, 2019年09月, ［査読有り］, ［国際誌］
Multiple genes, whose functions or expression are overlapping, compensate for the loss of one gene. A gene cluster in the mouse genome encodes five seminal vesicle proteins (SVS2, SVS3, SVS4, SVS5, and SVS6). These proteins are produced by male rodents and function in formation of the copulatory plug following mating. SVS2 plays an essential role in the successful internal fertilization by protecting the sperm membrane against a uterine immune attack. We hypothesized that the four remaining seminal vesicle proteins (SVPs) of this gene cluster may partially/completely compensate for the deficiency of SVS2. For confirming our hypothesis, we generated mice lacking the entire SVP-encoding gene cluster and compared their fecundity with Svs2-deficient (Svs2-/-) mice; that is, mice deficient in Svs2 alone. A single loxP site remained after the deletion of the Svs2 gene. Therefore, we inserted another loxP site by combining the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides (ssODN). Male mice lacking the entire SVP-encoding gene cluster (Svs2-6-/- mice) and thereby all five SVP proteins, generated by the deletion of 100kbp genomic DNA, showed low fecundity. However, the fecundity level was comparable with that from Svs2-/- male mice. Our results demonstrate that SVS3, SVS4, SVS5, and SVS6 do not function in the protection of sperm against a uterine immune attack in the absence of SVS2. Thus, Svs2 is the critical gene in the SVP gene cluster.
英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.3390/ijms20184557
DOI ID：10.3390/ijms20184557, PubMed ID：31540031, PubMed Central ID：PMC6769479 - Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods
Hibino T; Minokawa T; Yamazaki A
Methods in Cell Biology, 巻：150, 開始ページ：81, 終了ページ：103, 2019年01月, ［査読有り］
DOI：https://doi.org/10.1016/bs.mcb.2018.09.012
DOI ID：10.1016/bs.mcb.2018.09.012, ORCID：57103723, SCOPUS ID：85056708456 - IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva
Katherine M. Buckley; Eric Chun Hei Ho; Taku Hibino; Catherine S. Schrankel; Nicholas W. Schuh; Guizhi Wang; Jonathan P. Rast
ELIFE, 巻：6, 号：e23481, 開始ページ：1, 終了ページ：24, 2017年04月, ［査読有り］
IL17 cytokines are central mediators of mammalian immunity. In vertebrates, these factors derive from diverse cellular sources. Sea urchins share a molecular heritage with chordates that includes the IL17 system. Here, we characterize the role of epithelial expression of IL17 in the larval gut-associated immune response. The purple sea urchin genome encodes 10 IL17 subfamilies (35 genes) and 2 IL17 receptors. Most of these subfamilies are conserved throughout echinoderms. Two IL17 subfamilies are sequentially strongly upregulated and attenuated in the gut epithelium in response to bacterial disturbance. IL17R1 signal perturbation results in reduced expression of several response genes including an IL17 subtype, indicating a potential feedback. A third IL17 subfamily is activated in adult immune cells indicating that expression in immune cells and epithelia is divided among families. The larva provides a tractable model to investigate the regulation and consequences of gut epithelial IL17 expression across the organism.
ELIFE SCIENCES PUBLICATIONS LTD, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.7554/eLife.23481
DOI ID：10.7554/eLife.23481, ISSN：2050-084X, ORCID：57103731, SCOPUS ID：85020441127, Web of Science ID：WOS:000402554100001 - Perturbation of gut bacteria induces a coordinated cellular immune response in the purple sea urchin larva
Eric C. H. Ho; Katherine M. Buckley; Catherine S. Schrankel; Nicholas W. Schuh; Taku Hibino; Cynthia M. Solek; Koeun Bae; Guizhi Wang; Jonathan P. Rast
IMMUNOLOGY AND CELL BIOLOGY, 巻：94, 号：9, 開始ページ：861, 終了ページ：874, 2016年10月, ［査読有り］
The purple sea urchin (Strongylocentrotus purpuratus) genome sequence contains a complex repertoire of genes encoding innate immune recognition proteins and homologs of important vertebrate immune regulatory factors. To characterize how this immune system is deployed within an experimentally tractable, intact animal, we investigate the immune capability of the larval stage. Sea urchin embryos and larvae are morphologically simple and transparent, providing an organism-wide model to view immune response at cellular resolution. Here we present evidence for immune function in five mesenchymal cell types based on morphology, behavior and gene expression. Two cell types are phagocytic; the others interact at sites of microbial detection or injury. We characterize immune-associated gene markers for three cell types, including a perforin-like molecule, a scavenger receptor, a complement-like thioester-containing protein and the echinoderm-specific immune response factor 185/333. We elicit larval immune responses by (1) bacterial injection into the blastocoel and (2) seawater exposure to the marine bacterium Vibrio diazotrophicus to perturb immune state in the gut. Exposure at the epithelium induces a strong response in which pigment cells (one type of immune cell) migrate from the ectoderm to interact with the gut epithelium. Bacteria that accumulate in the gut later invade the blastocoel, where they are cleared by phagocytic and granular immune cells. The complexity of this coordinated, dynamic inflammatory program within the simple larval morphology provides a system in which to characterize processes that direct both aspects of the echinoderm-specific immune response as well as those that are shared with other deuterostomes, including vertebrates.
NATURE PUBLISHING GROUP, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1038/icb.2016.51
DOI ID：10.1038/icb.2016.51, ISSN：0818-9641, eISSN：1440-1711, ORCID：57103719, SCOPUS ID：84977068191, Web of Science ID：WOS:000386789200007 - Early stalked stages in ontogeny of the living isocrinid sea lily Metacrinus rotundus
Shonan Amemiya; Akihito Omori; Toko Tsurugaya; Taku Hibino; Masaaki Yamaguchi; Ritsu Kuraishi; Masato Kiyomoto; Takuya Minokawa
ACTA ZOOLOGICA, 巻：97, 号：1, 開始ページ：102, 終了ページ：116, 2016年01月, ［査読有り］
The early stalked stages of an isocrinid sea lily, Metacrinus rotundus, were examined up to the early pentacrinoid stage. Larvae induced to settle on bivalve shells and cultured in the laboratory developed into late cystideans. Three-dimensional (3D) images reconstructed from very early to middle cystideans indicated that 15 radial podia composed of five triplets form synchronously from the crescent-shaped hydrocoel. The orientation of the hydrocoel indicated that the settled postlarvae lean posteriorly. In very early cystideans, the orals, radials, basals and infrabasals, with five plates each in the crown, about five columnals in the stalk, and five terminal stem plates in the attachment disc, had already formed. In mid-cystideans, an anal plate appeared in the crown. Late cystideans cultured in the field developed into pentacrinoids about 5 months after settlement. These pentacrinoids shared many crown structures with adult sea lilies. On the other hand, many features of the stalk differed from those in adult isocrinids, while sharing many characteristics with the stalk of feather star pentacrinoids, including disc-like proximal columnals, high and slender median columnals, synarthrial articulations developmentally derived from the symplexial articulations, limited formation of cirri only in the proximal columnal(s), and an attachment disc. On the basis of these findings, phylogenetic relationships among extant crinoid orders are discussed.
WILEY-BLACKWELL, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1111/azo.12109
DOI ID：10.1111/azo.12109, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103720, SCOPUS ID：84954392628, Web of Science ID：WOS:000368010100010 - Development ciliary bands in larvae. of the living isocrinid sea lily Metacrinus rotundus
Shonan Amemiya; Taku Hibino; Hiroaki Nakano; Masaaki Yamaguchi; Ritsu Kuraishi; Masato Kiyomoto
ACTA ZOOLOGICA, 巻：96, 号：1, 開始ページ：37, 終了ページ：44, 2015年01月, ［査読有り］
Embryos and larvae of an isocrinid sea lily, Metacrinus rotundus, arc described by scanning electron microscopy. Around hatching (35 h after fertilization), the outer surface of the gastrula becomes ubiquitously covered with short cilia. At 40 h, the hatched swimming embryo develops a cilia free zone of ectoderm on the ventral side. By 3 days, the very early dipleurula larva develops a cilia free zone ventrally, densely ciliated regions laterally, and a sparsely ciliated region dorsally. At this stage, the posterior and anterior ciliary bands first appear: the former runs along a low ridge separating the densely from the sparsely ciliated epidermal regions, while the latter is visible, at first discontinuously, along the boundary between the densely ciliated lateral regions and the cilia-free ventral zone. In the late dipleurula larva (5 days after fertilization), the anterior and posterior loops of ciliary bands are 1,yell defined. The transition from the dipleurula to the semidoliolaria larva occurs at 6 days as the posterior loop becomes rearranged to form incompletely circumferential ciliary bands. The larva becomes competent to settle at this stage. The arrangement of the ciliary bands on the semidoliolaria is maintained during the second week of development, 1,vhile the larva retains its competence to settle. The larval ciliary patterns described here are compared with those of stalkless crinoids and eleutherozoan echinoderms. The closest morphological similarities are between M. rotundus and the basal eleutherozoan class Asteroidea.
WILEY-BLACKWELL, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1111/azo.12049
DOI ID：10.1111/azo.12049, ISSN：0001-7272, eISSN：1463-6395, ORCID：57103724, PubMed ID：25641974, SCOPUS ID：84919835996, Web of Science ID：WOS:000346775600004 - Seminal vesicle protein SVS2 is required for sperm survival in the uterus
Natsuko Kawano; Naoya Araki; Kaoru Yoshida; Taku Hibino; Naoko Ohnami; Maako Makino; Seiya Kanai; Hidetoshi Hasuwa; Manabu Yoshida; Kenji Miyado; Akihiro Umezawa
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 巻：111, 号：11, 開始ページ：4145, 終了ページ：4150, 2014年03月, ［査読有り］
In mammals, sperm migrate through the female reproductive tract to reach the egg; however, our understanding of this journey is highly limited. To shed light on this process, we focused on defining the functions of seminal vesicle secretion 2 (SVS2). SVS2(-/-) male mice produced sperm but were severely subfertile, and formation of a copulatory plug to cover the female genital opening did not occur. Surprisingly, even when artificial insemination was performed with silicon as a substitute for the plug, sperm fertility in the absence of SVS2 remained severely reduced because the sperm were already dead in the uterus. Thus, our results provide evidence that the uterus induces sperm cell death and that SVS2 protects sperm from uterine attack.
NATL ACAD SCIENCES, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1073/pnas.1320715111
DOI ID：10.1073/pnas.1320715111, ISSN：0027-8424, ORCID：57103717, PubMed ID：24591616, SCOPUS ID：84896533547, Web of Science ID：WOS:000333027900061 - A novel third complement component C3 gene of Ciona intestinalis expressed in the endoderm at the early developmental stages
T. Hibino; M. Nonaka
ISJ-INVERTEBRATE SURVIVAL JOURNAL, 巻：10, 号：1, 開始ページ：29, 終了ページ：37, 2013年03月, ［査読有り］, ［筆頭著者］
The third complement component (C3) in ascidian was reported to function as an opsonin to enhance phagocytosis and as a chemotactic factor for phagocytes, indicating that ascidian C3 works in mesodermal cavity as a humoral factor like vertebrate C3s. In the basal Eumetazoa, Cnidaria lacking mesodermal tissues, C3 was reported to work in an endodermal cavity. Evolution of structure and function of C3 is still to be clarified. Here we report the identification of the third C3 gene, CiC3-3, in the genome of an ascidian, Ciona intestinalis. Phylogenetic analysis using the entire amino acid sequences of Eumetazoan C3s indicated that CiC3-3 possess a closer relationship to vertebrate C3, C4 and C5 than other ascidian C3s. Although CiC3-3 retained the alpha-beta processing site and 6 cysteine residues in the C3a region, it lacked the intra-molecular thioester bond and the catalytic histidine residue. Instead, CiC3-3 had a unique insertion of about 70 residues long Lys/Arg-rich sequence. CiC3-3 was expressed highly in the embryonic stages, but little in the adult in contradistinction to CiC3-1 and CiC3-2. The expression of CiC3-3 in early embryonic stages was restricted to endoderm similar to cnidarian C3s. Thus, the ascidian complement system could represent a unique evolutionary stage sharing a primitive endodermal function with Cnidaria, and newly developed humoral function with vertebrates.
INVERTEBRATE SURVIVAL JOURNAL, 英語, 研究論文（学術雑誌）
ISSN：1824-307X, ORCID：57103711, SCOPUS ID：84925273676, Web of Science ID：WOS:000322360500004 - Sp185/333: A novel family of genes and proteins involved in the purple sea urchin immune response
Julie Ghosh; Katherine M. Buckley; Sham V. Nair; David A. Raftos; Chase Miller; Audrey J. Majeske; Taku Hibino; Jonathan P. Rast; Mattias Roth; L. Courtney Smith
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 巻：34, 号：3, 開始ページ：235, 終了ページ：245, 2010年03月, ［査読有り］
The Sp185/333 system of genes, messages and proteins are expressed in the coelomocytes of the purple sea urchin, Strongylocentrotus purpuratus, and is an extraordinary example of diversification of a putative innate immune response system in an invertebrate. Reviewed here, is the current understanding of this complex system as illustrated by sequence comparisons of the genes, messages and deduced proteins with descriptions of diversity, including preliminary results on genomic organization and descriptions of 185/333 in other echinoids. Sp185/333 gene expression in adults and embryos occurs in response to immune challenge and includes changes in the frequencies of Sp185/333-positive coelomocytes in the adults. The diversity of the Sp185/333 protein repertoire in coelomocytes is far greater than the sequence diversity encoded in the genes, which may be the result of rapid gene recombination, RNA editing and/or low-fidelity transcription, plus post-translational modifications. This review concludes with preliminary results and speculations on protein function. (C) 2009 Elsevier Ltd. All rights reserved.
ELSEVIER SCI LTD, 英語
DOI：https://doi.org/10.1016/j.dci.2009.10.008
DOI ID：10.1016/j.dci.2009.10.008, ISSN：0145-305X, ORCID：57103728, PubMed ID：19887082, SCOPUS ID：73249143320, Web of Science ID：WOS:000274832700001 - ウニの左右非対称性から見る後口動物の体軸の進化 (特集 生物はなぜ左右非対称なのか?--その仕組みを探る)
日比野 拓
細胞工学, 巻：27, 号：6, 開始ページ：548, 終了ページ：552, 2008年06月, ［招待有り］
秀潤社, 日本語
ISSN：0287-3796, CiNii Articles ID：40016077805, CiNii Books ID：AN00069897 - Phylogenetic correspondence of the body axes in bilaterians is revealed by the right-sided expression of Pitx genes in echinoderm larvae
Taku Hibino; Atsuo Nishino; Shonan Amemiya
DEVELOPMENT GROWTH & DIFFERENTIATION, 巻：48, 号：9, 開始ページ：587, 終了ページ：595, 2006年12月, ［査読有り］, ［筆頭著者］
Chordates and echinoderms are two of the three major deuterostome phyla and show conspicuous left-right (LR) asymmetry. The establishment of LR asymmetry has been explored in vertebrates, but is largely unknown in echinoderms. Here, we report the expression pattern of genes that are orthologous to the chordate left-side specific gene Pitx, cloned from the sea urchin Hemicentrotus pulcherrimus (HpPitx) and the starfish Asterina pectinifera (ApPitx). HpPitx transcripts were first detected bilaterally in one cell of the ventrolateral primary mesenchyme-cell aggregate of early prism larvae. New expression was detected asymmetrically in the right counterpart of a bilateral pair of mesodermal coelomic pouches and in the right ectoderm. In starfish bipinnaria larvae, the ApPitx signal was detected in the right coelomic pouch and in the right half of the ectoderm along the ciliary bands. These results suggest that the function of Pitx in establishing LR asymmetry was introduced in the last common ancestor of echinoderms and chordates. However, the right-side specific expression in echinoderm larvae is inverted compared to chordate embryos. This indicates that the LR axis is inversely represented between echinoderms and chordates, which supports the scenario that dorsoventral axis inversion was introduced into the chordate lineage by turning upside down.
BLACKWELL PUBLISHING, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1111/j.1440-169x.2006.00892.x
DOI ID：10.1111/j.1440-169x.2006.00892.x, ISSN：0012-1592, CiNii Articles ID：10018808295, ORCID：57103733, PubMed ID：17118013, SCOPUS ID：33750892936, Web of Science ID：WOS:000241914700004 - The immune gene repertoire encoded in the purple sea urchin genome
Taku Hibino; Mariano Loza-Coll; Cynthia Messier; Audrey J. Majeske; Avis H. Cohen; David P. Terwilliger; Katherine M. Buckley; Virginia Brockton; Sham V. Nair; Kevin Berney; Sebastian D. Fugmann; Michele K. Anderson; Zeev Pancer; R. Andrew Cameron; L. Courtney Smith; Jonathan P. Rast
DEVELOPMENTAL BIOLOGY, 巻：300, 号：1, 開始ページ：349, 終了ページ：365, 2006年12月, ［査読有り］, ［筆頭著者］
Echinoderms occupy a critical and largely unexplored phylogenetic vantage point from which to infer both the early evolution of bilaterian immunity and the underpinnings of the vertebrate adaptive immune system. Here we present an initial survey of the purple sea urchin genome for genes associated with immunity. An elaborate repertoire of potential immune receptors, regulators and effectors is present, including unprecedented expansions of innate pathogen recognition genes. These include a diverse array of 222 Toll-like receptor (TLR) genes and a coordinate expansion of directly associated signaling adaptors. Notably, a subset of sea urchin TLR genes encodes receptors with structural characteristics previously identified only in protostomes. A similarly expanded set of 203 NOD/NALP-like cytoplasmic recognition proteins is present. These genes have previously been identified only in vertebrates where they are represented in much lower numbers. Genes that mediate the alternative and lectin complement pathways are described, while gene homologues of the terminal pathway are not present. We have also identified several homologues of genes that function in jawed vertebrate adaptive immunity. The most striking of these is a gene cluster with similarity to the jawed vertebrate Recombination Activating Genes 1 and 2 (RAG1/2). Sea urchins are long-lived, complex organisms and these findings reveal an innate immune system of unprecedented complexity. Whether the presumably intense selective processes that molded these gene families also gave rise to novel immune mechanisms akin to adaptive systems remains to be seen. The genome sequence provides immediate opportunities to apply the advantages of the sea urchin model toward problems in developmental and evolutionary immunobiology. (c) 2006 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, 英語
DOI：https://doi.org/10.1016/j.ydbio.2006.08.065
DOI ID：10.1016/j.ydbio.2006.08.065, ISSN：0012-1606, eISSN：1095-564X, ORCID：57103721, PubMed ID：17027739, SCOPUS ID：33751549306, Web of Science ID：WOS:000242873300027 - RTK and TGF-beta signaling pathways genes in the sea urchin genome
Francois Lapraz; Eric Rottinger; Veronique Duboc; Ryan Range; Louise Duloquin; Katherine Walton; Shu-Yu Wu; Cynthia Bradham; Mariano A. Loza; Taku Hibino; Karen Wilson; Albert Poustka; Dave McClay; Lynne Angerer; Christian Gache; Thierry Lepage
DEVELOPMENTAL BIOLOGY, 巻：300, 号：1, 開始ページ：132, 終了ページ：152, 2006年12月, ［査読有り］
The Receptor Tyrosine kinase (RTK) and TGF-beta signaling pathways play essential roles during development in many organisms and regulate a plethora of cellular responses. From the genome sequence of Strongylocentrotus purpuratus, we have made an inventory of the genes encoding receptor tyrosine kinases and their ligands, and of the genes encoding cytokines of the TGF-beta superfamily and their downstream components.
The sea urchin genome contains at least 20 genes coding for canonical receptor tyrosine kinases. Seventeen of the nineteen vertebrate RTK families are represented in the sea urchin. Fourteen of these RTK among which ALK, CCK4/PTK7, DDR, EGFR, EPH, LMR, MET/RON, MUSK, RET, ROR, ROS, RYK, TIE and TRK are present as single copy genes while pairs of related genes are present for VEGFR, FGFR and INSR.
Similarly, nearly all the subfamilies of TGF-beta ligands identified in vertebrates are present in the sea urchin genome including the BMP, ADMP, GDF, Activin, Myostatin, Nodal and Lefty, as well as the TGF-beta sensu stricto that had not been characterized in invertebrates so far. Expression analysis indicates that the early expression of nodal, BMP2/4 and lefty is restricted to the oral ectoderm reflecting their role in providing positional information along the oral-aboral axis of the embryo. The coincidence between the emergence of TGF-beta-related factors such as Nodal and Lefty and the emergence of the deuterostome lineage strongly suggests that the ancestral function of Nodal could have been related to the secondary opening of the mouth which characterizes this clade, a hypothesis supported by functional data in the extant species.
The sea urchin genome contains 6 genes encoding TGF-beta receptors and 4 genes encoding prototypical Smad proteins. Furthermore, most of the transcriptional activators and repressors shown to interact with Smads in vertebrates have orthologues in echinoderms. Finally, the sea urchin genome contains an almost complete repertoire of genes encoding extracellular modulators of BMP signaling including Chordin, Noggin, Sclerotin, SFRP, Gremlin, DAN and Twisted gastrulation. Taken together, these findings indicate that the sea urchin complement of genes of the RTK and TGF-beta signaling pathways is qualitatively very similar to the repertoire present in vertebrates, and that these genes are part of the common genetool kit for intercellular signaling of deuterostomes. (c) 2006 Elsevier Inc. All rights reserved.
ACADEMIC PRESS INC ELSEVIER SCIENCE, 英語
DOI：https://doi.org/10.1016/j.ydbio.2006.08.048
DOI ID：10.1016/j.ydbio.2006.08.048, ISSN：0012-1606, eISSN：1095-564X, CiNii Articles ID：80018716920, ORCID：57103716, PubMed ID：17084834, SCOPUS ID：33846808910, Web of Science ID：WOS:000242873300013 - Research article - The genome of the sea urchin Strongylocentrotus purpuratus
Erica Sodergren; George M. Weinstock; Eric H. Davidson; R. Andrew Cameron; Richard A. Gibbs; George M. Weinstock; Robert C. Angerer; Lynne M. Angerer; Maria Ina Arnone; David R. Burgess; Robert D. Burke; R. Andrew Cameron; James A. Coffman; Eric H. Davidson; Michael Dean; Maurice R. Elphick; Charles A. Ettensohn; Kathy R. Foltz; Amro Hamdoun; Richard O. Hynes; William H. Klein; William Marzluff; David R. McClay; Robert L. Morris; Arcady Mushegian; Jonathan P. Rast; Erica Sodergren; L. Courtney Smith; Michael C. Thorndyke; Victor D. Vacquier; George M. Weinstock; Gary M. Wessel; Greg Wray; Lan Zhang; Erica Sodergren; George M. Weinstock; Robert C. Angerer; Lynne M. Angerer; R. Andrew Cameron; Eric H. Davidson; Christine G. Elsik; Olga Ermolaeva; Wratko Hlavina; Gretchen Hofmann; Paul Kitts; Melissa J. Landrum; Aaron J. Mackey; Donna Maglott; Georgia Panopoulou; Albert J. Poustka; Kim Pruitt; Victor Sapojnikov; Xingzhi Song; Alexandre Souvorov; Victor Solovyev; Zheng Wei; Charles A. Whittaker; Kim Worley; Lan Zhang; Erica Sodergren; George M. Weinstock; K. James Durbin; Richard A. Gibbs; Yufeng Shen; Xingzhi Song; Kim Worley; Lan Zhang; Greg Wray; Olivier Fedrigo; David Garfield; Ralph Haygood; Alexander Primus; Rahul Satija; Tonya Severson; Lan Zhang; Erica Sodergren; George M. Weinstock; Manuel L. Gonzalez-Garay; Andrew R. Jackson; Aleksandar Milosavljevic; Xingzhi Song; Mark Tong; Kim Worley; Charles A. Ettensohn; R. Andrew Cameron; Christopher E. Killian; Melissa J. Landrum; Brian T. Livingston; Fred H. Wilt; James A. Coffman; William Marzluff; Arcady Mushegian; Nikki Adams; Robert Belle; Seth Carbonneau; Rocky Cheung; Patrick Cormier; Bertrand Cosson; Jenifer Croce; Antonio Fernandez-Guerra; Anne-Marie Geneviere; Manisha Goel; Hemant Kelkar; Julia Morales; Odile Mulner-Lorillon; Anthony J. Robertson; Amro Hamdoun; Jared V. Goldstone; Nikki Adams; Bryan Cole; Michael Dean; David Epel; Bert Gold; Mark E. Hahn; Meredith Howard-Ashby; Mark Scally; John J. Stegeman; Robert L. Morris; Erin L. Allgood; Jonah Cool; Kyle M. Judkins; Shawn S. McCafferty; Ashlan M. Musante; Robert A. Obar; Amanda P. Rawson; Blair J. Rossetti; David R. Burgess; Erin L. Allgood; Jonah Cool; Ian R. Gibbons; Matthew P. Hoffman; Kyle M. Judkins; Andrew Leone; Shawn S. McCafferty; Robert L. Morris; Ashlan M. Musante; Robert A. Obar; Amanda P. Rawson; Blair J. Rossetti; Gary M. Wessel; Eric H. Davidson; R. Andrew Cameron; Sorin Istrail; Stefan C. Materna; Manoj P. Samanta; Viktor Stolc; Waraporn Tongprasit; Qiang Tu; Robert C. Angerer; Lynne M. Angerer; Zheng Wei; Richard O. Hynes; Karl-Frederik Bergeron; Bruce P. Brandhorst; Robert D. Burke; Charles A. Whittaker; James Whittle; R. Andrew Cameron; Kevin Berney; David J. Bottjer; Cristina Calestani; Eric H. Davidson; Kevin Peterson; Elly Chow; Qiu Autumn Yuan; Eran Elhaik; Christine G. Elsik; Dan Graur; Justin T. Reese; Ian Bosdet; Shin Heesun; Marco A. Marra; Jacqueline Schein; Michael Dean; Amro Hamdoun; Jonathan P. Rast; L. Courtney Smith; Michele K. Anderson; Kevin Berney; Virginia Brockton; Katherine M. Buckley; R. Andrew Cameron; Avis H. Cohen; Sebastian D. Fugmann; Taku Hibino; Mariano Loza-Coll; Audrey J. Majeske; Cynthia Messier; Sham V. Nair; Zeev Pancer; David P. Terwilliger; Robert D. Burke; Maurice R. Elphick; William H. Klein; Michael C. Thorndyke; Cavit Agca; Lynne M. Angerer; Enrique Arboleda; Maria Ina Arnone; Bruce P. Brandhorst; Nansheng Chen; Allison M. Churcher; F. Hallboeoek; Glen W. Humphrey; Richard O. Hynes; Mohammed M. Idris; Takae Kiyama; Shuguang Liang; Dan Mellott; Xiuqian Mu; Greg Murray; Robert P. Olinski; Florian Raible; Matthew Rowe; John S. Taylor; Kristin Tessmar-Raible; D. Wang; Karen H. Wilson; Shunsuke Yaguchi; Kathy R. Foltz; Victor D. Vacquier; Gary M. Wessel; Terry Gaasterland; Blanca E. Galindo; Herath J. Gunaratne; Meredith Howard-Ashby; Glen W. Humphrey; Celina Juliano; Masashi Kinukawa; Gary W. Moy; Anna T. Neill; Mamoru Nomura; Michael Raisch; Anna Reade; Michelle M. Roux; Jia L. Song; Yi-Hsien Su; Ian K. Townley; Ekaterina Voronina; Julian L. Wong; Maria Ina Arnone; Michael C. Thorndyke; Gabriele Amore; Lynne M. Angerer; Enrique Arboleda; Margherita Branno; Euan R. Brown; Vincenzo Cavalieri; Veronique Duboc; Louise Duloquin; Maurice R. Elphick; Constantin Flytzanis; Christian Gache; Anne-Marie Geneviere; Mohammed M. Idris; Francois Lapraz; Thierry Lepage; Annamaria Locascio; Pedro Martinez; Giorgio Matassi; Valeria Matranga; David R. McClay; Julia Morales; Albert J. Poustka; Florian Raible; Ryan Range; Francesca Rizzo; Eric Roettinger; Matthew Rowe; Kristin Tessmar-Raible; Erica Sodergren; George M. Weinstock; Karen Wilson; David R. McClay; Lynne M. Angerer; Maria Ina Arnone; Wendy Beane; Cynthia Bradham; Christine Byrum; Jenifer Croce; Veronique Duboc; Louise Duloquin; Christian Gache; Anne-Marie Geneviere; Tom Glenn; Taku Hibino; Sofia Hussain; Francois Lapraz; Thierry Lepage; Brian T. Livingston; Mariano Loza; Gerard Manning; Esther Miranda; Ryan Range; Francesca Rizzo; Eric Roettinger; Rebecca Thomason; Katherine Walton; Zheng Wei; Gary M. Wessel; Athula Wikramanayke; Karen H. Wilson; Charles Whittaker; Shu-Yu Wu; Ronghui Xu; Eric H. Davidson; Maria Ina Arnone; Margherita Branno; C. Titus Brown; R. Andrew Cameron; Lili Chen; Rachel F. Gray; Meredith Howard-Ashby; Sorin Istrail; Pei Yun Lee; Annamaria Locascio; Pedro Martinez; Stefan C. Materna; Jongmin Nam; Paola Oliveri; Francesca Rizzo; Joel Smith; Donna Muzny; Erica Sodergren; Richard A. Gibbs; George M. Weinstock; Stephanie Bell; Joseph Chacko; Andrew Cree; Stacey Curry; Clay Davis; Huyen Dinh; Shannon Dugan-Rocha; Jerry Fowler; Rachel Gill; Cerrissa Hamilton; Judith Hernandez; Sandra Hines; Jennifer Hume; LaRonda Jackson; Angela Jolivet; Christie Kovar; Sandra Lee; Lora Lewis; George Miner; Margaret Morgan; Lynne V. Nazareth; Geoffrey Okwuonu; David Parker; Ling-Ling Pu; Yufeng Shen; Rachel Thom; Rita Wright
SCIENCE, 巻：314, 号：5801, 開始ページ：941, 終了ページ：952, 2006年11月, ［査読有り］
AMER ASSOC ADVANCEMENT SCIENCE, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1126/science.1133609
DOI ID：10.1126/science.1133609, ISSN：0036-8075, CiNii Articles ID：30020565990, ORCID：57103713, PubMed ID：17095691, SCOPUS ID：33750995860, Web of Science ID：WOS:000241896000039 - Review - Genomic insights into the immune system of the sea urchin
Jonathan P. Rast; L. Courtney Smith; Mariano Loza-Coll; Taku Hibino; Gary W. Litman
SCIENCE, 巻：314, 号：5801, 開始ページ：952, 終了ページ：956, 2006年11月, ［査読有り］
Comparative analysis of the sea urchin genome has broad implications for the primitive state of deuterostome host defense and the genetic underpinnings of immunity in vertebrates. The sea urchin has an unprecedented complexity of innate immune recognition receptors relative to other animal species yet characterized. These receptor genes include a vast repertoire of 222 Toll-like receptors, a superfamily of more than 200 NACHT domain - leucine- rich repeat proteins ( similar to nucleotide-binding and oligomerization domain ( NOD) and NALP proteins of vertebrates), and a large family of scavenger receptor cysteine-rich proteins. More typical numbers of genes encode other immune recognition factors. Homologs of important immune and hematopoietic regulators, many of which have previously been identified only from chordates, as well as genes that are critical in adaptive immunity of jawed vertebrates, also are present. The findings serve to underscore the dynamic utilization of receptors and the complexity of immune recognition that may be basal for deuterostomes and predicts features of the ancestral bilaterian form.
AMER ASSOC ADVANCEMENT SCIENCE, 英語
DOI：https://doi.org/10.1126/science.1134301
DOI ID：10.1126/science.1134301, ISSN：0036-8075, CiNii Articles ID：80018776789, ORCID：57103725, PubMed ID：17095692, SCOPUS ID：33750980112, Web of Science ID：WOS:000241896000040 - Ion flow regulates left-right asymmetry in sea urchin development
T Hibino; Y Ishii; M Levin; A Nishino
DEVELOPMENT GENES AND EVOLUTION, 巻：216, 号：5, 開始ページ：265, 終了ページ：276, 2006年05月, ［査読有り］, ［筆頭著者］
The degree of conservation among phyla of early mechanisms that pattern the left - right (LR) axis is poorly understood. Larvae of sea urchins exhibit consistently oriented LR asymmetry. The main part of the adult rudiment is formed from the left coelomic sac of larvae, the left hydrocoel. Although this left preference is conserved among all echinoderm larvae, its mechanism is largely not understood. Using two marker genes, HpNot and HpFoxFQ-like, which are asymmetrically expressed during larval development of the sea urchin Hemicentrotus pulcherrimus, we examined in this study the possibility that the recently discovered ion flux mechanism controls asymmetry in sea urchins as it does in several vertebrate species. Several ion-transporter inhibitors were screened for the ability to alter the expression of the asymmetric marker genes. Blockers of the H+/K+-ATPase ( omeprazole, lansoprazole and SCH28080), as well as a calcium ionophore (A23187), significantly altered the normal sidedness of asymmetric gene expression. Exposure to omeprazole disrupted the consistent asymmetry of adult rudiment formation in larvae. Immuno-detection revealed that H+/K+-ATPase-like antigens in sea urchin embryos were present through blastula stage and exhibited a striking asymmetry being present in a single blastomere in 32-cell embryos. These results suggest that, as in vertebrates, endogenous spatially-regulated early transport of H+ and/or K+, and also of Ca2+, functions in the establishment of LR asymmetry in sea urchin development.
SPRINGER, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1007/s00427-005-0051-6
DOI ID：10.1007/s00427-005-0051-6, ISSN：0949-944X, CiNii Articles ID：10024443340, ORCID：57103722, PubMed ID：16534626, SCOPUS ID：33645870420, Web of Science ID：WOS:000236967500004 - Molecular heterotopy in the expression of Brachyury orthologs in order Clypeasteroida (irregular sea urchins) and order Echinoida (regular sea urchins)
T Hibino; Y Harada; T Minokawa; M Nonaka; S Amemiya
DEVELOPMENT GENES AND EVOLUTION, 巻：214, 号：11, 開始ページ：546, 終了ページ：558, 2004年11月, ［査読有り］, ［筆頭著者］
The expression patterns of Brachyury (Bra) orthologs in the development of four species of sand dollars (order: Clypeasteroida), including a direct-developing species, and of a sea urchin species (order: Echinoida) were investigated during the period from blastula to the pluteus stage, with special attention paid to the relationship between the expression pattern and the mode of gastrulation. The sand dollar species shared two expression domains of the Bra orthologs with the Echinoida species, in the vegetal ring (the first domain) and the oral ectoderm (the second domain). The following heterotopic changes in the expression of the Bra genes were found among the sand dollar species and between the sand dollars and the Echinoida species. (1) The vegetal ring expressing Bra in the sand dollars was much wider and was located at a higher position along the AV axis, compared with that in the Echinoida species. The characteristic Bra expression in the vegetal ring of the sand dollar embryos was thought to be involved in the mode of gastrulation, in which involution continues from the beginning of invagination until the end of gastrulation. (2) Two of the three indirect-developing sand dollar species that were examined exhibited a third domain, in which Bra was expressed on the oral side of the archenteron. (3) In the direct-developing sand dollar embryos, Bra was expressed with an oral-aboral asymmetry in the vegetal ring and with a left-right asymmetry in the oral ectoderm. In the Echinoida species, Bra was expressed in the vestibule at the six-armed pluteus stage.
SPRINGER, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1007/s00427-004-0437-x
DOI ID：10.1007/s00427-004-0437-x, ISSN：0949-944X, ORCID：57103715, SCOPUS ID：8544284103, Web of Science ID：WOS:000224954800003 - Regrowth of the stalk of the sea lily, Metacrinus rotundus (Echinodermata : crinoidea)
H Nakano; T Hibino; Y Hara; T Oji; S Amemiya
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-COMPARATIVE EXPERIMENTAL BIOLOGY, 巻：301A, 号：6, 開始ページ：464, 終了ページ：471, 2004年06月, ［査読有り］
Sea lilies are critical to understanding the evolution of the echinoderm body plan, because they are the only extant group whose adults possess a stalk, a prevalent feature in the radiation of a number of primitive echinoderm lineages. Extensive crown regeneration ability has been reported in Metacrinus rotundus, but the regenerative potential of the stalk has never been determined in any species of sea lilies. In this study, we show that M. rotundus whose stalks have been completely excised are capable of stalk regeneration. The process is similar to the growth of the original stalk, but much slower, and the regenerated stalks are not morphologically identical to the original stalk. Since stalk regeneration, in contrast to well-studied regeneration events, probably requires little additional activation of morphogenetic programs, we refer to the stalk regeneration phenomenon as "stalk regrowth" to distinguish it as a special form of regeneration. Since specimens whose entire stalk below the basal plates had been removed were able to regrow, the basal plates, and probably the aboral nerve center within them, are essential For stalk regrowth. Sea lily stalk regrowth is described in detail, and the evolution of feather stars is discussed in light of the growth pattern of the sea lily stalk. (C) 2004 Wiley-Liss, Inc.
WILEY-LISS, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1002/jez.a.77
DOI ID：10.1002/jez.a.77, ISSN：0022-104X, ORCID：57103729, SCOPUS ID：3042850763, Web of Science ID：WOS:000221985500002 - Development of the sea lily Metacrinus rotundus: Comparisons with feather stars
Nakano, H; Hibino, T; Hara, Y; Oji, T; Amemiya, S
Proceedings of the 11th International Echinoderm Conference, 開始ページ：41-44, 2004年01月, ［査読有り］
英語, 研究論文（国際会議プロシーディングス） - Larval stages of a living sea lily (stalked crinoid echinoderm)
H Nakano; T Hibino; T Oji; Y Hara; S Amemiya
NATURE, 巻：421, 号：6919, 開始ページ：158, 終了ページ：160, 2003年01月, ［査読有り］
The embryos and larvae of stalked crinoids, which are considered the most basal group of extant echinoderms(1,2), have not previously been described. In contrast, much is known about the development of the more accessible stalkless crinoids (feather stars)(3), which are phylogenetically derived from stalked forms(4). Here we describe the development of a sea lily from fertilization to larval settlement. There are two successive larval stages: the first is a non-feeding auricularia stage with partly longitudinal ciliary bands (similar to the auricularia and bipinnaria larvae of holothurian and asteroid echinoderms, respectively); the second is a doliolaria larva with circumferential ciliary bands (similar to the earliest larval stage of stalkless crinoids). We suggest that a dipleurula-type larva is primitive for echinoderms and is the starting point for the evolution of additional larval forms within the phylum. From a wider evolutionary viewpoint, the demonstration that the most basal kind of echinoderm larva is a dipleurula is consistent with Garstang's auricularia theory(5) for the phylogenetic origin of the chordate neural tube.
NATURE PUBLISHING GROUP, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1038/nature01236
DOI ID：10.1038/nature01236, ISSN：0028-0836, CiNii Articles ID：80015736591, ORCID：57103726, PubMed ID：12520300, SCOPUS ID：0346634891, Web of Science ID：WOS:000180267200038 - The behavior and the morphology of sea lilies with shortened stalks: Implications on the evolution of feather stars
H Nakano; T Hibino; Y Hara; T Oji; S Amemiya
ZOOLOGICAL SCIENCE, 巻：19, 号：8, 開始ページ：961, 終了ページ：964, 2002年08月, ［査読有り］
Extant crinoids can be divided into two groups, stalked sea lilies and stalkless feather stars. Feather stars are considered to have evolved from stalked ancestors by losing most of the stalk, but other differences are present between the two groups. The unsegmented centrodorsal, long and curved cirri near the crown, small calyx, and the ability to swim are all feather star features not found in the sea lilies. To figure out which of the above features evolved directly correlating with loss of the stalk in feather stars, we cut off the stalk from the sea lily Metacrinus rotundus and kept them alive in an aquarium. The specimens with shortened stalks were able to stand and crawl with their arms without the support of their stalks, but swimming was not observed for any of the animals. Morphologically, neither fusion of the remaining segments nor the reduction of the size of the calyx were observed, but the cirri became long and curved near the crown. Therefore, the extant sea lilies possess a potential to adapt to incidents of stalk loss. Specimens autotomizing most of their stalks were observed, suggesting that the potential is actually employed in nature. This mechanism linking the reduction of the stalk and the changes in the morphology of cirri may have played an important role in the evolution of the feather stars, if the stalked ancestors of feather stars also possessed this potential. Experimental zoological approaches as this study may provide new insights to the questions of evolution.
ZOOLOGICAL SOC JAPAN, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.2108/zsj.19.961
DOI ID：10.2108/zsj.19.961, ISSN：0289-0003, CiNii Articles ID：110003372800, ORCID：57103714, PubMed ID：12193811, SCOPUS ID：0036703130, Web of Science ID：WOS:000179250500014 - The centrosome-attracting body, microtubule system, and posterior egg cytoplasm are involved in positioning of cleavage planes in the ascidian embryo
T Nishikata; T Hibino; H Nishida
DEVELOPMENTAL BIOLOGY, 巻：209, 号：1, 開始ページ：72, 終了ページ：85, 1999年05月, ［査読有り］
Many kinds of animal embryos exhibit stereotyped cleavage patterns during early embryogenesis. In the ascidian Halocynthia roretzi, cleavage patterns are invariant but they are complicated by successive unequal cleavages that occur in the posterior region. Here we, report the essential roles of a novel structure, called the centrosome-attracting body (CAB), which exists in the posterior pole cortex of cleaving embryos, in generating unequal cleavages. By removing and transplanting posterior egg cytoplasm and by treatment with sodium dodecyl sulfate, we demonstrated that loss of the CAB resulted in abolishment of unequal cleavage, while ectopic formation of the CAB caused ectopic unequal cleavages to occur. Experiments with a microtubule inhibitor demonstrated that the centrosome and nucleus were attracted toward the posterior cortex, where the CAB is located, by shortening of microtubule bundles formed between the centrosome and the CAB. Consequently, the mitotic apparatus was positioned asymmetrically, resulting in unequal cleavage. Immunohistochemistry provided evidence that a microtubule motor protein, a kinesin or kinesin-like molecule, may be associated with the CAB. formation of the CAB during the early cleavage stage was resistant to treatment with the microtubule inhibitor. In contrast, the integrity of the CAB was lost upon treatment with a microfilament inhibitor. We propose that the CAB plays key roles in the orientation and positioning of cleavage planes during unequal cell division. (C) 1999 Academic Press.
ACADEMIC PRESS INC, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1006/dbio.1999.9244
DOI ID：10.1006/dbio.1999.9244, ISSN：0012-1606, CiNii Articles ID：80011048021, ORCID：57103727, PubMed ID：10208744, SCOPUS ID：0033135884, Web of Science ID：WOS:000079924400007 - Centrosome-attracting body: A novel structure closely related to unequal cleavages in the ascidian embryo
T Hibino; T Nishikata; H Nishida
DEVELOPMENT GROWTH & DIFFERENTIATION, 巻：40, 号：1, 開始ページ：85, 終了ページ：95, 1998年02月, ［査読有り］
The mechanism of unequal cleavage is one of the most intriguing subjects in cell biology. Previous studies of unequal cleavage have focused on a limited number of organisms such as yeasts, nematodes, sea urchins and annelids. The cleavage pattern of the ascidian embryo is invariant. In the ascidian embryo, the posterior-most blastomeres divide unequally in three successive cleavages. In the present study, it was shown that the ascidian embryo provides another good experimental system with which to analyze the mechanism of unequal cleavage. A novel structure, designated as CAB (centrosome-attracting body), which was found specifically in the unequally cleaving blastomeres was described. In the course of unequal cleavages, first, a thick microtubule bundle appeared between CAB and one of the centrosomes. Then with the shortening of the microtubule bundle, the nucleus with the centrosome was drawn toward CAB, situated at the posterior cortex of the blastomere. Finally, a cleavage furrow formed in the middle of the asymmetrically located mitotic apparatus and produced two blastomeres of different size, generating a smaller cell that inherits CAB. The CAB seemed to play an essential role in the unequal cleavages in the ascidian embryo.
BLACKWELL PUBLISHING ASIA, 英語, 研究論文（学術雑誌）
DOI：https://doi.org/10.1046/j.1440-169X.1998.t01-5-00010.x
DOI ID：10.1046/j.1440-169X.1998.t01-5-00010.x, ISSN：0012-1592, ORCID：57103732, SCOPUS ID：0031943947, Web of Science ID：WOS:000072500000010
- 環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較<数学・自然科学>
吉田 竜矢; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, 巻：68, 号：1, 開始ページ：317, 終了ページ：331, 2019年
埼玉大学教育学部, 日本語
ISSN：1881-5146, CiNii Articles ID：120006585307 - ムジナモ自生地緊急調査後４年間の宝蔵寺沼水生動物相の変遷<数学・自然科学>
吉田 竜矢; 宇田川 貴大; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, 巻：67, 号：2, 開始ページ：341, 終了ページ：351, 2018年
埼玉大学教育学部, 日本語
ISSN：1881-5146, CiNii Articles ID：120006533500 - 私の実験動物＃５ タコノマクラ
日比野 拓
JADCI News, 巻：49, 開始ページ：20, 終了ページ：23, 2018年 - 免疫カードゲーム教材の開発
日比野 拓
JADCI News, 巻：46, 開始ページ：5, 終了ページ：6, 2017年06月
日本語 - 地域に密着した環境保全をテーマとした学習指導の開発 : ムジナモ自生地宝蔵寺沼の水生動物相調査を事例として<数学・自然科学>
吉田 竜矢; 田端 雄樹; 伊藤 悠昭; 山本 孔紀; 矢辺 徹; 金子 康子; 日比野 拓
埼玉大学紀要. 教育学部 = Journal of Saitama University. Faculty of Education, 巻：66, 号：2, 開始ページ：609, 終了ページ：622, 2017年
埼玉大学教育学部, 日本語
ISSN：1881-5146, CiNii Articles ID：120006389474 - 自然免疫の異物識別機構への理解を促すTLRカードゲームの開発<数学・自然科学>
日比野 拓
埼玉大学紀要. 教育学部, 巻：65, 号：2, 開始ページ：261, 終了ページ：270, 2016年10月
Toll-like receptors (TLRs) are protein sensors located on immune cells, which function as pathogen recognition by the innate immune system. These TLRs form a homodimer or a heterodimer, which recognizes distinct pathogen-associated molecular patterns. The explanation of the TLRs and their function have been introduced on Japanese biological textbooks for high school students since 2012. Here, I developed a card game, TLR Card Game, as an educational material for understanding the mechanism of the innate pathogen recognition. The TLR Card Game is for multiplayers who are surrounding a table where several pairs of a phagocytic cell board and a pathogen card are set. Each player has to present two TLR cards from his own hand in his turn, making a TLR dimer corresponding to a pathogen card presenting on the table. When a TLR card-pair is presented correctly, a player can get several points, and the player with highest points finally gets win. The TLR Card Game was carried out in an extracurricular class of a high school in Saitama City. The effectiveness of the game was assessed by open- and close-ended questionnaires for 30 students. All the students got interested in the TLR Card Game and its immunology. Some students, however, answered that the game and the immune mechanism were not much linked in their thought. The TLR Card Game is considered useful for students attracted towards immunology, but the instructions before use needed to be improved.
埼玉大学教育学部, 日本語
ISSN：1881-5146, CiNii Articles ID：120005853513 - 実験・観察の活動を取り入れた生活科授業―教科内容学模索の取り組み―
鶴ヶ谷柊子; 日比野拓
埼玉大学教育学部附属教育実践総合センター紀要, 号：15, 開始ページ：135‐140, 終了ページ：140, 2016年02月26日
近年、教科専門と教科教育を架橋する分野として「教科内容学」の研究が進んでいる。小学校低学年の教科である生活科は「具体的な活動や体験」を通した学びが重視されている。本研究では、教科内容学の視点から、生活科の講義における、実験・観察の活動の導入と学生の生活科に対する理解の関連について実践と検討を行った。その結果、具体的な活動を講義に取り入れることは有効であるが、受講者の持つ知識に応じて、実験・観察の活動内容を組み立てることが、生活科の特徴への理解を深める上でも必要であることがわかった。
埼玉大学教育学部, 日本語
ISSN：1347-7420, J-Global ID：201702290329001002, CiNii Articles ID：120005818438, CiNii Books ID：AA11948173 - ムジナモ保全のための遺伝的多様性の解析
日比野 拓; 川合 真紀
宝蔵寺沼ムジナモ自生地緊急調査報告書 発行：羽生市教育委員会, 開始ページ：72, 終了ページ：81, 2015年
日本語 - 水生動物相の変遷とムジナモ食害の調査
日比野 拓; 宇田川 貴大; 伊藤 悠昭; 矢辺 徹; 梅沢 一弘; 関森 清己
宝蔵寺沼ムジナモ自生地緊急調査報告書 発行：羽生市教育委員会, 開始ページ：82, 終了ページ：96, 2015年
日本語 - ウニ幼生における貪食作用に関わる細胞の同定
日比野 拓
埼玉大学総合研究機構,平成20年度総合研究機構プロジェクト研究報告書, 巻：7, 2009年
日本語 - ウニ幼生の左右非対称性から後口動物の体軸の進化を考える
日比野 拓
海鞘, 巻：21, 開始ページ：1, 終了ページ：4, 2009年
日本語, 記事・総説・解説・論説等（その他） - CHANGES IN THE MORPHOLOGY OF SEA LILIES WITH SHORTENED STALKS(Taxonomy and Systematics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :
Nakano Hiroaki; Hibino Taku; Hara Yuko; Oji Tatsuo; Amemiya Shonan
Zoological science, 巻：19, 号：12, 開始ページ：1425, 終了ページ：1425, 2002年
Zoological Society of Japan, 英語
ISSN：0289-0003, CiNii Articles ID：110003376235, CiNii Books ID：AA10545874 - DEVELOPMENT OF THE STALKED CRINOID, METACRINUS ROTUNDUS(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan) :
Nakano Hiroaki; Hara Yuko; Oji Tatsuo; Amemiya Shonan; Hibino Taku
Zoological science, 巻：18, 開始ページ：79, 終了ページ：79, 2001年
Zoological Society of Japan, 英語
ISSN：0289-0003, CiNii Articles ID：110003372443, CiNii Books ID：AA10545874
- ぼくらは「生物学」のおかげで生きている (素晴らしきサイエンス)
金子康子; 日比野拓, ［共著］
実務教育出版, 2015年12月
総ページ数：232
ISBN：4788911701, ASIN：4788911701, EAN：9784788911703
■ 共同研究・競争的資金等の研究課題
- 科学と人間生活との関わりを理解し生きる力を育む教材開発
日本学術振興会, 科学研究費助成事業, 基盤研究(C), 2021年04月01日 - 2024年03月31日
日比野 拓, 埼玉大学
配分額（総額）：3380000, 配分額（直接経費）：2600000, 配分額（間接経費）：780000
高等学校理科の科目の一つである「科学と人間生活」の学習内容に沿って、日常生活から科学を考える教材を開発したり、動画を作成したりすることが本研究の目的である。科学と人間生活の生物分野では、免疫のしくみ、特にアレルギーや予防接種のしくみを学習するが、これらの観察・実験の事例は乏しい。なぜなら、ヒトを含む哺乳類を実験材料として用いることが難しいからである。
（１）アレルギー反応ではマスト細胞が脱顆粒を起こし、化学物質を放出する。本研究者は、ウニ幼生においても、色素細胞が脱顆粒を起こして、化学物質を放出することを発見し、その瞬間を動画として撮影することができた。この結果を2021年8月に開催された第32回日本比較免疫学会において、「ウニ発生における胞胚腔細胞と色素／顆粒細胞のはたらき」というタイトルで口頭発表した。今後はウニのこの現象をもとにして、私たちの体内でおこる免疫現象をいかに伝えるのかを考え、動画や教材を作成してていく。
（２）ひらめき☆ときめきサイエンス「ウニは食べずにカガクする―受精と免疫の観察実験―」を2021年12月に開催し、本研究者が開発したカードゲーム教材「免疫理解で大冒険」を試行した。これまで高校生にこの教材を試行したことはあったが、今回中学生を対象に教材を試行した。私たちの日常生活と免疫とのかかわりに対して、生徒の興味関心を引き出すことができた。
課題番号：21K02898 - ウニの後胚発生機構の研究
日本学術振興会, 科学研究費助成事業, 基盤研究(C), 2018年04月01日 - 2021年03月31日
美濃川 拓哉; 日比野 拓; 根岸 剛文, 東北大学
配分額（総額）：4420000, 配分額（直接経費）：3400000, 配分額（間接経費）：1020000
本研究は、幼生とはなにか、成体とはなにか、成体を形作るしくみとはどのようなものか、という後胚発生に関連する問題を、【1】正常発生における成体原基の形成過程と構造の解析と、【2】再生・出芽実験系を利用した胚発生と後胚発生の機構的関連の理解、の２課題に注目して検討した。【1】については消化管の構造解析を行った。その結果、幼生から成体への変態期における消化管の形態変化に新たな特徴を発見した。 【2】については幼生の再生実験系を用いて、幼生構成細胞が後胚発生に関与するかどうかを検証した。幼生は、その消化管の大部分を失っても、欠損部を再生し、機能的・構造的に正常な消化管を再構築できることを確かめた。
課題番号：18K06307 - 免疫システムと社会をつなぐ教材の開発と普及
日本学術振興会, 科学研究費補助金(基盤研究(C)), 2017年04月 - 2020年03月
日比野 拓, 研究代表者
競争的資金 - 免疫にかかわる細胞や分子への理解を促す学習教材の開発
日本学術振興会, 科学研究費補助金(基盤研究(C)), 基盤研究(C), 2014年04月 - 2017年03月
日比野 拓, 埼玉大学, 研究代表者
配分額（総額）：4810000, 配分額（直接経費）：3700000, 配分額（間接経費）：1110000
トル様受容体（TLR）は免疫細胞上のセンサータンパク質であり、自然免疫において病原体を認識する役割を果たす。この免疫のしくみを理解させるため、「TLRカードゲーム」を開発し、現職高校教員への普及に努めた。さいたま市の高校で授業実践を行い、生徒30名からアンケート結果を得た。すべての生徒がこの教材とその免疫のしくみに関心を示した。次に免疫のしくみと社会や生活とを結びつけるボードゲーム教材「免疫理解で大冒険」を開発した。最後に食作用をライブで観察できる実験系を開発するため、3種のウニの胚・幼生を比較実験した。タコノマクラ胚の胞胚腔内では、迅速な食作用が起こり、教材化に適していることが分かった。
競争的資金, 課題番号：26350225 - 自然免疫における祖先型TIRドメインをもつシグナル分子の機能と分子進化の解析
日本学術振興会, 科学研究費補助金(若手研究(B)), 若手研究(B), 2010年04月 - 2012年03月
日比野 拓, 埼玉大学, 研究代表者
配分額（総額）：4290000, 配分額（直接経費）：3300000, 配分額（間接経費）：990000
後口動物の祖先のToll様受容体やTIRドメインを含むシグナル分子は、どのような遺伝子構成をしていたのかを明らかにするために、棘皮動物の系統でもっとも基部に位置づけられている有柄ウミユリ類トリノアシの胚・幼生cDNAの解析を行った。これまでに、トリノアシに6種類のToll様受容体(3種類のハエタイプ、3種類の脊椎動物タイプ)の存在を確認した。またMyD88-like, Sarm-likeといったウニとナメクジウオに共通した遺伝子だけでなく、ウニにはないがナメクジウオには存在するTIRドメインを含む遺伝子であるCARD-TIR、TPR-TIRがトリノアシに存在することが分かった。このことから、後口動物の祖先は多様なTIRドメインを含む遺伝子を保有していたが、その後TLRのシグナル伝達系が洗練されていくにつれて、それらの遺伝子は失われたと考えられた。
競争的資金, 課題番号：22770232 - 驚きと感動をつたえ理科離れを未然に防ぐ理科大好きプロジェクト
日本学術振興会, 科学研究費助成事業, 基盤研究(B), 2009年 - 2012年
芦田 実; 大向 隆三; 日比野 拓; 岡本 和明; 清水 誠; 片平 克弘; 谷塚 光典; 芦田 正巳, 埼玉大学
配分額（総額）：19240000, 配分額（直接経費）：14800000, 配分額（間接経費）：4440000
理科離れを防ぐために種々の事業を実施した.お助け隊として小学校に学生を派遣し,理科室の整理や授業の補助に従事させた.科学館の行事,三郷市の遊学館や川越市のふれあい事業等にも派遣した.観察実験教室では附属小学校や騎西小学校等で種々の驚きと感動を伝えた.また,天体観望会やワークショップを開催した.ホームページで多数の質問に回答し,水溶液の作り方,実験のシミュレーションや理科カレンダーを公開した.
課題番号：21300288 - 棘皮動物ゲノムにおけるToll-like receptor多重遺伝子族の分子進化
日本学術振興会, 科学研究費補助金(若手研究(スタートアップ)), 若手研究(スタートアップ), 2008年04月 - 2010年03月
日比野 拓, 埼玉大学, 研究代表者
配分額（総額）：3302000, 配分額（直接経費）：2540000, 配分額（間接経費）：762000
ショウジョウバエとヒトは約10種類のToll-like receptor(TLR)を保有している。一方、ウニ・ナメクジウオ・ゴカイといった海産無脊椎動物は、70～200種類のTLRを持ち、多重遺伝子族を形成している。TLRや自然免疫関連遺伝子の分子進化を調べたところ、ハエ・ヒトには見られず、上記の海産無脊椎動物にのみ見られるIL-17遺伝子の重複とSarm-like遺伝子群を発見した。これら遺伝子群はTLR多重遺伝子族の形成と関わっている可能性が高い。
競争的資金, 課題番号：20870007 - 免疫細胞分化の遺伝子制御ネットワーク解析
上原記念生命科学財団, 海外留学助成ポストドクトラルフェローシップ, 2005年04月 - 2006年03月
日比野 拓, 研究代表者
競争的資金 - -
競争的資金