Performance information

• Neofunctionalization of VAMP7 opened up a plant-unique vacuolar transport pathway　　　　　　　　　　　　　　　
  Masaru Fujimoto; Yutaro Shimizu; Yoko Ito; Kazuo Ebine; Naoki Minamino; Takehiko Kanazawa; Yoichiro Fukao; Akihiko Nakano; Tomohiro Uemura; Takashi Ueda
  Current Biology, May 2025, [Reviewed]
  Elsevier BV, English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2025.04.062
  DOI ID：10.1016/j.cub.2025.04.062, ISSN：0960-9822, PubMed ID：40367944, 共同研究・競争的資金等ID：33075853
• Characterization of Subcellular Dynamics of Sterol Methyltransferases Clarifies Defective Cell Division in smt2 smt3, a C-24 Ethyl Sterol-Deficient Mutant of Arabidopsis.　　　　　　　　　　　　　　　
  Daisaku Ohta; Ayaka Fuwa; Yuka Yamaroku; Kazuki Isobe; Masatoshi Nakamoto; Atsushi Okazawa; Takumi Ogawa; Kazuo Ebine; Takashi Ueda; Pierre Mercier; Hubert Schaller
  Biomolecules, Volume：14, Number：7, Jul. 2024, ［International magazine］
  An Arabidopsis sterol mutant, smt2 smt3, defective in sterolmethyltransferase2 (SMT2), exhibits severe growth abnormalities. The loss of C-24 ethyl sterols, maintaining the biosynthesis of C-24 methyl sterols and brassinosteroids, suggests specific roles of C-24 ethyl sterols. We characterized the subcellular localizations of fluorescent protein-fused sterol biosynthetic enzymes, such as SMT2-GFP, and found these enzymes in the endoplasmic reticulum during interphase and identified their movement to the division plane during cytokinesis. The mobilization of endoplasmic reticulum-localized SMT2-GFP was independent of the polarized transport of cytokinetic vesicles to the division plane. In smt2 smt3, SMT2-GFP moved to the abnormal division plane, and unclear cell plate ends were surrounded by hazy structures from SMT2-GFP fluorescent signals and unincorporated cellulose debris. Unusual cortical microtubule organization and impaired cytoskeletal function accompanied the failure to determine the cortical division site and division plane formation. These results indicated that both endoplasmic reticulum membrane remodeling and cytokinetic vesicle transport during cytokinesis were impaired, resulting in the defects of cell wall generation. The cell wall integrity was compromised in the daughter cells, preventing the correct determination of the subsequent cell division site. We discuss the possible roles of C-24 ethyl sterols in the interaction between the cytoskeletal network and the plasma membrane.
  English, Scientific journal
  DOI：https://doi.org/10.3390/biom14070868
  DOI ID：10.3390/biom14070868, PubMed ID：39062582, PubMed Central ID：PMC11275053
• A bHLH heterodimer regulates germ cell differentiation in land plant gametophytes.　　　　　　　　　　　　　　　
  Misaki Saito; Ryosuke Momiki; Kazuo Ebine; Yoshihiro Yoshitake; Ryuichi Nishihama; Takuya Miyakawa; Takeshi Nakano; Nobutaka Mitsuda; Takashi Araki; Takayuki Kohchi; Shohei Yamaoka
  Current biology : CB, Sep. 2023, ［International magazine］
  Land plants are a monophyletic group of photosynthetic eukaryotes that diverged from streptophyte algae about 470 million years ago. During both the alternating haploid and diploid stages of the life cycle, land plants form multicellular bodies.1,2,3,4 The haploid multicellular body (gametophyte) produces progenitor cells that give rise to gametes and the reproductive organs.5,6,7,8 In the liverwort Marchantia polymorpha, differentiation of the initial cells of gamete-producing organs (gametangia) from the gametophyte is regulated by MpBONOBO (MpBNB), a member of the basic helix-loop-helix (bHLH) transcription factor subfamily VIIIa. In Arabidopsis thaliana, specification of generative cells in developing male gametophytes (pollen) requires redundant action of BNB1 and BNB2.9 Subfamily XI bHLHs, such as LOTUS JAPONICUS ROOTHAIRLESS LIKE1 (LRL1)/DEFECTIVE REGION OF POLLEN1 (DROP1) and LRL2/DROP2 in A. thaliana and the single LRL/DROP protein MpLRL in M. polymorpha, are the evolutionarily conserved regulators of rooting system development.10 Although the role of LRL1/DROP1 and LRL2/DROP2 in gametogenesis remains unclear, their loss leads to the formation of abnormal pollen devoid of sperm cells.11 Here, we show that BNBs and LRL/DROPs co-localize to gametophytic cell nuclei and form heterodimers. LRL1/DROP1 and LRL2/DROP2 act redundantly to regulate BNB expression for generative cell specification in A. thaliana after asymmetric division of the haploid microspore. MpLRL is required for differentiation of MpBNB-expressing gametangium initial cells in M. polymorpha gametophytes. Our findings suggest that broadly expressed LRL/DROP stabilizes BNB expression, leading to the formation of an evolutionarily conserved bHLH heterodimer, which regulates germ cell differentiation in the haploid gametophyte of land plants.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2023.09.020
  DOI ID：10.1016/j.cub.2023.09.020, PubMed ID：37776860
• The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis.　　　　　　　　　　　　　　　
  Tomoko Hirano; Kazuo Ebine; Takashi Ueda; Takumi Higaki; Takahiro Watanabe-Nakayama; Hiroki Konno; Hisako Takigawa-Imamura; Masa H Sato
  The Plant cell, Sep. 2023, ［International magazine］
  The extended tubular shape of root hairs is established by tip growth and concomitant hardening. Here, we demonstrate that a syntaxin of plants (SYP)123-vesicle-associated membrane protein (VAMP)727-dependent secretion system delivers secondary cell wall components for hardening the subapical zone and shank of Arabidopsis (Arabidopsis thaliana) root hairs. We found increased SYP123 localization at the plasma membrane of the subapical and shank zones compared to the tip region in elongating root hairs. Inhibition of phosphatidylinositol (PtdIns)(3,5)P2 production impaired SYP123 localization at the plasma membrane and SYP123-mediated root hair shank hardening. Moreover, root hair elongation in the syp123 mutant was insensitive to a PtdIns(3,5)P2 synthesis inhibitor. SYP123 interacts with both VAMP721 and VAMP727. syp123 and vamp727 mutants exhibited reduced shank cell wall stiffness due to impaired secondary cell wall component deposition. Based on these results, we conclude that SYP123 is involved in VAMP721-mediated conventional secretion for root hair elongation as well as in VAMP727-mediated secretory functions for the delivery of secondary cell wall components to maintain root hair tubular morphology.
  English, Scientific journal
  DOI：https://doi.org/10.1093/plcell/koad240
  DOI ID：10.1093/plcell/koad240, PubMed ID：37713604
• Membrane trafficking functions of the ANTH/ENTH/VHS domain-containing proteins in plants.　　　　　　　　　　　　　　　
  Yihong Feng; Takuma Hiwatashi; Naoki Minamino; Kazuo Ebine; Takashi Ueda
  FEBS letters, May 2022, ［International magazine］
  Subcellular localization of proteins acting on the endomembrane system is primarily regulated via membrane trafficking. To obtain and maintain the correct protein composition of the plasma membrane and membrane-bound organelles, the loading of selected cargos into transport vesicles is critically regulated at donor compartments by adaptor proteins binding to the donor membrane, the cargo molecules, and the coat-protein complexes, including the clathrin coat. The ANTH/ENTH/VHS domain-containing protein superfamily generally comprises a structurally related ENTH, ANTH, or VHS domain in the N-terminal region and a variable C-terminal region, which is thought to act as an adaptor during transport vesicle formation. This protein family is involved in various plant processes, including pollen tube growth, abiotic stress response, and development. In this review, we provide an overview of the recent findings on ANTH/ENTH/VHS domain-containing proteins in plants.
  English, Scientific journal
  DOI：https://doi.org/10.1002/1873-3468.14368
  DOI ID：10.1002/1873-3468.14368, PubMed ID：35505466
• Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis　　　　　　　　　　　　　　　
  Shizuka Koshimizu; Naoki Minamino; Tomoaki Nishiyama; Emiko Yoro; Kazuo Ebine; Keiko Sakakibara; Takashi Ueda; Kentaro Yano
  The New phytologist, Volume：236, Number：3, First page：1182, Last page：1196, Jul. 2021, ［International magazine］
  Oogamy is a form of sexual reproduction and evolved independently in animals, fungi, and plants. In streptophyte plants, Charophyceae, Coleochaetophyceae, bryophytes, lycophytes, ferns (monilophytes), and some gymnosperms (Cycads and Ginkgo) utilize spermatozoids as the male gamete. Plant spermatozoids commonly possess characteristic structures such as the spline, which consists of a microtubule array, the multilayered structure (MLS) in which the uppermost layer is continuum of the spline, and multiple flagella. However, the molecular mechanisms underpinning plant spermatogenesis remain to be elucidated. To identify the genes involved in plant spermatogenesis, we performed computational analyses and successfully found deeply divergent BLD10s by combining multiple methods and omics-data. We then validated the functions of candidate genes in the liverwort Marchantia polymorpha and the moss Physcomitrium patens and found that MpBLD10 and PpBLD10 are required for normal basal body and flagella formation. Mpbld10 mutants exhibited defects in remodeling of the cytoplasm and nucleus during spermatozoid formation, thus MpBLD10 should be involved in chromatin reorganization and elimination of the cytoplasm during spermiogenesis. Streptophyte BLD10s are orthologous to BLD10/CEP135 family proteins, which function in basal body assembly, but we found that BLD10s evolved especially fast in land plants and MpBLD10 might obtain additional functions in spermatozoid formation through the fast molecular evolution. This study provides a successful example of combinatorial study from evolutionary and molecular genetic perspectives that elucidated a function of the key protein of the basal body formation that fast evolved in land plants.
  Cold Spring Harbor Laboratory, English, Scientific journal
  DOI：https://doi.org/10.1101/2021.07.25.453666
  DOI ID：10.1101/2021.07.25.453666, PubMed ID：35842793
• Remodeling of organelles and microtubules during spermiogenesis in the liverwort Marchantia polymorpha　　　　　　　　　　　　　　　
  Naoki Minamino; Takuya Norizuki; Shoji Mano; Kazuo Ebine; Takashi Ueda
  Development (Cambridge, England), Volume：149, Number：15, Jul. 2021, ［International magazine］
  Gametogenesis is an essential biological event for sexual reproduction in various organisms. Bryophytes and some other plants employ motile sperms (spermatozoids) as male gametes, which self-locomote to the egg cells to accomplish fertilization. Spermatozoids of bryophytes harbor distinctive morphological characteristics, including the cell body with a helical slender shape and two motile flagella at the anterior edge. During transformation from a spermatid to spermatozoid (spermiogenesis), the shape and cellular contents of spermatids are dynamically reorganized. However, how each organelle is reorganized during plant spermiogenesis remains obscure. In this study, we classified the developmental processes during spermiogenesis in the liverwort Marchantia polymorpha according to cellular and nuclear shapes and flagella development. We then examined the remodeling of microtubules and reorganization of endomembrane organelles during spermiogenesis. The results indicate that the state of post-translational modification of tubulin is dynamically changed during the formation of the flagella and spline, and the plasma membrane and endomembrane organelles are drastically reorganized in a precisely regulated manner during spermiogenesis. These findings are expected to provide useful indexes to classify developmental and subcellular processes of spermiogenesis in bryophytes.
  Cold Spring Harbor Laboratory, English, Scientific journal
  DOI：https://doi.org/10.1101/2021.07.10.451882
  DOI ID：10.1101/2021.07.10.451882, PubMed ID：35924955
• Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants.　　　　　　　　　　　　　　　
  Yutaro Shimizu; Junpei Takagi; Emi Ito; Yoko Ito; Kazuo Ebine; Yamato Komatsu; Yumi Goto; Mayuko Sato; Kiminori Toyooka; Takashi Ueda; Kazuo Kurokawa; Tomohiro Uemura; Akihiko Nakano
  Nature communications, Volume：12, Number：1, First page：1901, Last page：1901, Mar. 2021, ［International magazine］
  The trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different destinies still remains elusive. Here, we examined 3D localization and 4D dynamics of TGN-localized proteins of Arabidopsis thaliana that are involved in either secretory or vacuolar trafficking from the TGN, by a multicolor high-speed and high-resolution spinning-disk confocal microscopy approach that we developed. We demonstrate that TGN-localized proteins exhibit spatially and temporally distinct distribution. VAMP721 (R-SNARE), AP (adaptor protein complex)-1, and clathrin which are involved in secretory trafficking compose an exclusive subregion, whereas VAMP727 (R-SNARE) and AP-4 involved in vacuolar trafficking compose another subregion on the same TGN. Based on these findings, we propose that the single TGN has at least two subregions, or "zones", responsible for distinct cargo sorting: the secretory-trafficking zone and the vacuolar-trafficking zone.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-021-22267-0
  DOI ID：10.1038/s41467-021-22267-0, PubMed ID：33772008, PubMed Central ID：PMC7997971
• The SYP123-VAMP727 SNARE complex is involved in the delivery of inner cell wall components to the root hair shank in Arabidopsis
  Tomoko Hirano; Kazuo Ebine; Takashi Ueda; Takumi Higaki; Takahiro Nakayama; Hiroki Konno; Hisako Takigawa-Imamura; Masa H. Sato
  Dec. 2020
  A root hair is a long tubular protrusion from a root hair cell established via tip growth, which is accomplished by the polarized deposition of membranous and cell wall components at the root hair apex accompanied by simultaneous hardening of the shank. The polarized secretion of materials to the root hair apex is well investigated; however, little is known about the deposition of inner cell wall materials at the root hair shank. We have previously reported that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P₂)/ROP10 signaling is required for the regulation of cortical microtubule construction and the deposition of inner cell wall components at the root hair shank during hardening. To unravel the alternate secretion mechanism for delivery of the inner cell wall components to root hair shank, here, we demonstrate that root hair-specific Qa-SNARE, SYP123, localizes to the subapical zone and shank of elongating root hairs in Arabidopsis. SYP123-mediated root hair elongation was inhibited by the FAB1 inhibitor YM201636, and inhibition of PtdIns(3,5)P₂ production impaired the plasma membrane localization of SYP123. We also showed that SYP123 forms a SNARE complex with VAMP727 on the plasma membrane, and syp123 and vamp727 mutants exhibited lower cell wall stiffness in the root hair shank because of impaired deposition of inner cell wall components. These results indicate that SYP123/VAMP727-mediated secretion is involved in the transport of inner cell wall components for hardening of the root hair shank.
  Cold Spring Harbor Laboratory
  DOI：https://doi.org/10.1101/2020.12.28.424500
  DOI ID：10.1101/2020.12.28.424500
• The liverwort oil body is formed by redirection of the secretory pathway.　　　　　　　　　　　　　　　
  Takehiko Kanazawa; Hatsune Morinaka; Kazuo Ebine; Takashi L Shimada; Sakiko Ishida; Naoki Minamino; Katsushi Yamaguchi; Shuji Shigenobu; Takayuki Kohchi; Akihiko Nakano; Takashi Ueda
  Nature communications, Volume：11, Number：1, First page：6152, Last page：6152, Dec. 2020, ［International magazine］
  Eukaryotic cells acquired novel organelles during evolution through mechanisms that remain largely obscure. The existence of the unique oil body compartment is a synapomorphy of liverworts that represents lineage-specific acquisition of this organelle during evolution, although its origin, biogenesis, and physiological function are yet unknown. We find that two paralogous syntaxin-1 homologs in the liverwort Marchantia polymorpha are distinctly targeted to forming cell plates and the oil body, suggesting that these structures share some developmental similarity. Oil body formation is regulated by an ERF/AP2-type transcription factor and loss of the oil body increases M. polymorpha herbivory. These findings highlight a common strategy for the acquisition of organelles with distinct functions in plants, via periodical redirection of the secretory pathway depending on cellular phase transition.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41467-020-19978-1
  DOI ID：10.1038/s41467-020-19978-1, PubMed ID：33262353, PubMed Central ID：PMC7708844
• Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis
  Masaru Fujimoto; Kazuo Ebine; Kohji Nishimura; Nobuhiro Tsutsumi; Takashi Ueda
  Proceedings of the National Academy of Sciences, First page：202011152, Last page：202011152, Sep. 2020, [Reviewed]
  The plasma membrane (PM) acts as the interface between intra- and extracellular environments and exhibits a tightly regulated molecular composition. The composition and amount of PM proteins are regulated by balancing endocytic and exocytic trafficking in a cargo-specific manner, according to the demands of specific cellular states and developmental processes. In plant cells, retrieval of membrane proteins from the PM depends largely on clathrin-mediated endocytosis (CME). However, the mechanisms for sorting PM proteins during CME remain ambiguous. In this study, we identified a homologous pair of ANTH domain-containing proteins, PICALM1a and PICALM1b, as adaptor proteins for CME of the secretory vesicle-associated longin-type R-SNARE VAMP72 group. PICALM1 interacted with the SNARE domain of VAMP72 and clathrin at the PM. The loss of function of PICALM1 resulted in faulty retrieval of VAMP72, whereas general endocytosis was not considerably affected by this mutation. The double mutant of PICALM1 exhibited impaired vegetative development, indicating the requirement of VAMP72 recycling for normal plant growth. In the mammalian system, VAMP7, which is homologous to plant VAMP72, is retrieved from the PM via the interaction with a clathrin adaptor HIV Rev-binding protein in the longin domain during CME, which is not functional in the plant system, whereas retrieval of brevin-type R-SNARE members is dependent on a PICALM1 homolog. These results indicate that ANTH domain-containing proteins have evolved to be recruited distinctly for recycling R-SNARE proteins and are critical to eukaryote physiology.
  Proceedings of the National Academy of Sciences, Scientific journal
  DOI：https://doi.org/10.1073/pnas.2011152117
  DOI ID：10.1073/pnas.2011152117, ISSN：0027-8424, eISSN：1091-6490, ORCID：80891293
• Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination.　　　　　　　　　　　　　　　
  Alif Meem Nurani; Yasuko Ozawa; Tomoyuki Furuya; Yuki Sakamoto; Kazuo Ebine; Sachihiro Matsunaga; Takashi Ueda; Hiroo Fukuda; Yuki Kondo
  Plant & cell physiology, Volume：61, Number：2, First page：255, Last page：264, Feb. 2020, [Reviewed], ［Domestic magazine］
  Stem cells undergo cell division and differentiation to ensure organized tissue development. Because plant cells are immobile, plant stem cells ought to decide their cell fate prior to differentiation, to locate specialized cells in the correct position. In this study, based on a chemical screen, we isolated a novel secondary cell wall indicator BF-170, which binds to lignin and can be used to image in vitro and in situ xylem development. Use of BF-170 to observe the vascular differentiation pattern in the in vitro vascular cell induction system, VISUAL, revealed that adaxial mesophyll cells of cotyledons predominantly generate ectopic xylem cells. Moreover, phloem cells are abundantly produced on the abaxial layer, suggesting the involvement of leaf adaxial-abaxial polarity in determining vascular cell fate. Analysis of abaxial polarity mutants highlighted the role of YAB3, an abaxial cell fate regulator, in suppressing xylem and promoting phloem differentiation on the abaxial domains in VISUAL. Furthermore, YABBY family genes affected in vivo vascular development during the secondary growth. Our results denoted the possibility that such mediators of spatial information contribute to correctly determine the cell fate of vascular stem cells, to conserve the vascular pattern of land plants.
  English
  DOI：https://doi.org/10.1093/pcp/pcaa002
  DOI ID：10.1093/pcp/pcaa002, PubMed ID：31922574
• Enrichment of Phosphatidylinositol 4,5-Bisphosphate in the Extra-Invasive Hyphal Membrane Promotes Colletotrichum Infection of Arabidopsis thaliana.　　　　　　　　　　　　　　　
  Takashi L Shimada; Shigeyuki Betsuyaku; Noriko Inada; Kazuo Ebine; Masaru Fujimoto; Tomohiro Uemura; Yoshitaka Takano; Hiroo Fukuda; Akihiko Nakano; Takashi Ueda
  Plant & cell physiology, Volume：60, Number：7, First page：1514, Last page：1524, Jul. 2019, [Reviewed], ［Domestic magazine］
  Pathogenic fungi from the genus Colletotrichum form invasive hyphae; the hyphae are surrounded by an extra-invasive hyphal membrane (EIHM), which is continuous with the plant plasma membrane. Although the EIHM plays a crucial role as the interface between plant and fungal cells, its precise function during Colletotrichum infection remains elusive. Here, we show that enrichment of phosphoinositides (PIs) has a crucial role in Colletotrichum infection. We observed the localization of PIs in Arabidopsis thaliana cells infected by A. thaliana-adapted Colletotrichum higginsianum (Ch), and found that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] was extremely enriched in the EIHM during Ch infection. We also found that phosphatidylinositol 4-phosphate-5 kinase (PIP5K), which catalyzes production of PI(4,5)P2, also accumulated at the EIHM. The overexpression of PIP5K3 in A. thaliana increased hyphal invasion by Ch. An exocytic factor, EXO84b, was targeted to the EIHM during Ch infection, although endocytic factors such as CLATHRIN LIGHT CHAIN 2 and FLOTILLIN 1 did not. Intriguingly, the interfacial membranes between A. thaliana and powdery mildew- or downy mildew-causing pathogens did not accumulate PI(4,5)P2. These results suggest that Ch could modify the PI(4,5)P2 levels in the EIHM to increase the exocytic membrane/protein supply of the EIHM for successful infection. Our results also suggest that PI(4,5)P2 biosynthesis is a promising target for improved defense against Colletotrichum infection.
  English
  DOI：https://doi.org/10.1093/pcp/pcz058
  DOI ID：10.1093/pcp/pcz058, PubMed ID：30989198
• Transcriptional switch for programmed cell death in pith parenchyma of sorghum stems.　　　　　　　　　　　　　　　
  Masaru Fujimoto; Takashi Sazuka; Yoshihisa Oda; Hiroyuki Kawahigashi; Jianzhong Wu; Hideki Takanashi; Takayuki Ohnishi; Jun-Ichi Yoneda; Motoyuki Ishimori; Hiromi Kajiya-Kanegae; Ken-Ichiro Hibara; Fumiko Ishizuna; Kazuo Ebine; Takashi Ueda; Tsuyoshi Tokunaga; Hiroyoshi Iwata; Takashi Matsumoto; Shigemitsu Kasuga; Jun-Ichi Yonemaru; Nobuhiro Tsutsumi
  Proceedings of the National Academy of Sciences of the United States of America, Volume：115, Number：37, First page：E8783-E8792, Last page：E8792, Sep. 2018, [Reviewed], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1807501115
  DOI ID：10.1073/pnas.1807501115, ORCID：50019891, PubMed ID：30150370, PubMed Central ID：PMC6140496, SCOPUS ID：85053035451
• ANTH domain-containing proteins are required for the pollen tube plasma membrane integrity via recycling ANXUR kinases.　　　　　　　　　　　　　　　
  Muro K; Matsuura-Tokita K; Tsukamoto R; Kanaoka MM; Ebine K; Higashiyama T; Nakano A; Ueda T
  Communications biology, Volume：1, Sep. 2018, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/s42003-018-0158-8
  DOI ID：10.1038/s42003-018-0158-8, eISSN：2399-3642, ORCID：50019969, PubMed ID：30272028, PubMed Central ID：PMC6158268, Web of Science ID：WOS:000461126500152
• RAB GTPases in the Basal Land Plant Marchantia polymorpha
  Naoki Minamino; Takehiko Kanazawa; Atsuko Era; Kazuo Ebine; Akihiko Nakano; Takashi Ueda
  Plant and Cell Physiology, Volume：59, Number：4, First page：845, Last page：856, Apr. 2018, [Reviewed]
  Oxford University Press, English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcy027
  DOI ID：10.1093/pcp/pcy027, ISSN：1471-9053, ORCID：50019878, SCOPUS ID：85045524602
• Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases mediate membrane fusion at the vacuole in Arabidopsis
  Kodai Takemoto; Kazuo Ebine; Jana Christin Askani; Falco Krüger; Zaida Andrés Gonzalez; Emi Ito; Tatsuaki Goh; Karin Schumacher; Akihiko Nakano; Takashi Ueda
  Proceedings of the National Academy of Sciences of the United States of America, Volume：115, Number：10, First page：E2457, Last page：E2466, Mar. 2018, [Reviewed]
  National Academy of Sciences, English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1717839115
  DOI ID：10.1073/pnas.1717839115, ISSN：1091-6490, ORCID：50019890, SCOPUS ID：85042903326
• Integration of two RAB5 groups during endosomal transport in plants
  Ito E; Ebine K; Choi S.-W; Ichinose S; Uemura T; Nakano A; Ueda T
  eLife, Volume：7, 2018, [Reviewed]
  DOI：https://doi.org/10.7554/eLife.34064.001
  DOI ID：10.7554/eLife.34064.001, ORCID：50019904, SCOPUS ID：85051951897
• Triphosphate Tunnel Metalloenzyme Function in Senescence Highlights a Biological Diversification of This Protein Superfamily　　　　　　　　　　　　　　　
  Huoi Ung; Purva Karia; Kazuo Ebine; Takashi Ueda; Keiko Yoshioka; Wolfgang Moeder
  PLANT PHYSIOLOGY, Volume：175, Number：1, First page：473, Last page：485, Sep. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1104/pp.17.00700
  DOI ID：10.1104/pp.17.00700, ISSN：0032-0889, eISSN：1532-2548, ORCID：50019897, SCOPUS ID：85029233944, Web of Science ID：WOS:000408792200036
• Constitutive activation of plant-specific RAB5 GTPase confers increased resistance against adapted powdery mildew fungus　　　　　　　　　　　　　　　
  Noriko Inada; Kazuo Ebine; Emi Ito; Akihiko Nakano; Takashi Ueda
  PLANT BIOTECHNOLOGY, Volume：34, Number：2, First page：89, Last page：95, Jun. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.5511/plantbiotechnology.17.0501a
  DOI ID：10.5511/plantbiotechnology.17.0501a, ISSN：1342-4580, ORCID：50019903, SCOPUS ID：85021696796, Web of Science ID：WOS:000404743200003
• Modulation of Plant RAB GTPase-Mediated Membrane Trafficking Pathway at the Interface Between Plants and Obligate Biotrophic Pathogens　　　　　　　　　　　　　　　
  Noriko Inada; Shigeyuki Betsuyaku; Takashi L. Shimada; Kazuo Ebine; Emi Ito; Natsumaro Kutsuna; Seiichiro Hasezawa; Yoshitaka Takano; Hiroo Fukuda; Akihiko Nakano; Takashi Ueda
  PLANT AND CELL PHYSIOLOGY, Volume：57, Number：9, First page：1854, Last page：1864, Sep. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcw107
  DOI ID：10.1093/pcp/pcw107, ISSN：0032-0781, eISSN：1471-9053, ORCID：50019889, SCOPUS ID：84988407826, Web of Science ID：WOS:000384717400005
• Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2　　　　　　　　　　　　　　　
  Kazuo Ebine; Makoto Hirai; Miako Sakaguchi; Kazuhide Yahata; Osamu Kaneko; Yumiko Saito-Nakano
  MALARIA JOURNAL, Volume：15, Number：1, Jun. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1186/s12936-016-1377-4
  DOI ID：10.1186/s12936-016-1377-4, ISSN：1475-2875, ORCID：50019887, SCOPUS ID：84975815488, Web of Science ID：WOS:000378829700001
• AtNHX5 and AtNHX6 Are Required for the Subcellular Localization of the SNARE Complex That Mediates the Trafficking of Seed Storage Proteins in Arabidopsis　　　　　　　　　　　　　　　
  Xuexia Wu; Kazuo Ebine; Takashi Ueda; Quan-Sheng Qiu
  PLOS ONE, Volume：11, Number：3, Mar. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0151658
  DOI ID：10.1371/journal.pone.0151658, ISSN：1932-6203, ORCID：50019899, SCOPUS ID：84978151448, Web of Science ID：WOS:000372580300106
• Roles of membrane trafficking in plant cell wall dynamics　　　　　　　　　　　　　　　
  Kazuo Ebine; Takashi Ueda
  Frontiers in Plant Science, Volume：6, Number：OCTOBER, Oct. 2015, [Reviewed]
  English
  DOI：https://doi.org/10.3389/fpls.2015.00878
  DOI ID：10.3389/fpls.2015.00878, ISSN：1664-462X, ORCID：50019879, SCOPUS ID：84947445699, Web of Science ID：WOS:000364344600001
• Integral roles of membrane trafficking in biogenesis and modulation of the plant cell wall(The plant cell wall as an information processing system)　　　　　　　　　　　　　　　
  Ebine Kazuo; Ueda Takashi
  Regulation of Plant Growth & Development, Volume：50, Number：1, First page：43, Last page：49, 2015
  <p>The cell wall is a characteristic component of plant cells, whose structure and composition vary depending on the cell type, developmental stage, and environmental condition. For construction and proper modulation of the cell wall, a vast number of proteins and polysaccharides should be transported to the plasma membrane and/or the extracellular space correctly. The delivery of these materials depends on the membrane trafficking system, a key mechanism of intracellular transport connecting single-membrane bounded organelles and the extracellular space. Although molecular mechanisms of membrane trafficking are largely conserved in eukaryotic lineages, plants could have developed unique trafficking mechanisms to construct the cell wall and maintain its integrity, given the plant-specific nature of the cell wall. In this review, we summarize recent progress in the field of membrane trafficking in plant cells, especially focusing on the cell wall.</p>
  一般社団法人 植物化学調節学会, Japanese
  DOI：https://doi.org/10.18978/jscrp.50.1_43
  DOI ID：10.18978/jscrp.50.1_43, ISSN：1346-5406, CiNii Articles ID：110009969798, CiNii Books ID：AA11550064
• Plant Vacuolar Trafficking Occurs through Distinctly Regulated Pathways　　　　　　　　　　　　　　　
  Kazuo Ebine; Takeshi Inoue; Jun Ito; Emi Ito; Tomohiro Uemura; Tatsuaki Goh; Hiroshi Abe; Ken Sato; Akihiko Nakano; Takashi Ueda
  CURRENT BIOLOGY, Volume：24, Number：12, First page：1375, Last page：1382, Jun. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.cub.2014.05.004
  DOI ID：10.1016/j.cub.2014.05.004, ISSN：0960-9822, eISSN：1879-0445, ORCID：50019886, SCOPUS ID：84902684556, Web of Science ID：WOS:000337648200028
• Interactomics of Qa-SNARE in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Masayuki Fujiwara; Tomohiro Uemura; Kazuo Ebine; Yuka Nishimori; Takashi Ueda; Akihiko Nakano; Masa H. Sato; Yoichiro Fukao
  PLANT AND CELL PHYSIOLOGY, Volume：55, Number：4, First page：781, Last page：789, Apr. 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcu038
  DOI ID：10.1093/pcp/pcu038, ISSN：0032-0781, eISSN：1471-9053, ORCID：50019902, SCOPUS ID：84898905936, Web of Science ID：WOS:000334679500012
• Erratum: Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions (Nature (2008) 456 (962-966) DOI:10.1038/nature07409)
  Dhonukshe P; Tanaka H; Goh T; Ebine K; M{\"a}h{\"o}nen A.P; Prasad K; Blilou I; Geldner N; Xu J; Uemura T; Chory J; Ueda T; Nakano A; Scheres B; Friml J
  Nature, Volume：511, Number：7509, First page：370, 2014, [Reviewed]
  DOI：https://doi.org/10.1038/nature13549
  DOI ID：10.1038/nature13549, ORCID：50019884, SCOPUS ID：84904409041
• RABA Members Act in Distinct Steps of Subcellular Trafficking of the FLAGELLIN SENSING2 Receptor　　　　　　　　　　　　　　　
  Seung-won Choi; Takayuki Tamaki; Kazuo Ebine; Tomohiro Uemura; Takashi Ueda; Akihiko Nakano
  PLANT CELL, Volume：25, Number：3, First page：1174, Last page：1187, Mar. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.112.108803
  DOI ID：10.1105/tpc.112.108803, ISSN：1040-4651, ORCID：50019905, SCOPUS ID：84876758349, Web of Science ID：WOS:000318157100029
• Flowering Time Modulation by a Vacuolar SNARE via FLOWERING LOCUS C in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Kazuo Ebine; Tomohiro Uemura; Akihiko Nakano; Takashi Ueda
  PLOS ONE, Volume：7, Number：7, Jul. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0042239
  DOI ID：10.1371/journal.pone.0042239, ISSN：1932-6203, ORCID：50019901, SCOPUS ID：84864411840, Web of Science ID：WOS:000306950200187
• Statistical organelle dissection of Arabidopsis guard cells using image database LIPS　　　　　　　　　　　　　　　
  Takumi Higaki; Natsumaro Kutsuna; Yoichiroh Hosokawa; Kae Akita; Kazuo Ebine; Takashi Ueda; Noriaki Kondo; Seiichiro Hasezawa
  SCIENTIFIC REPORTS, Volume：2, May 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep00405
  DOI ID：10.1038/srep00405, ISSN：2045-2322, ORCID：50019906, SCOPUS ID：84861145038, Web of Science ID：WOS:000304044100002
• Qa-SNAREs localized to the trans-Golgi network regulate multiple transport pathways and extracellular disease resistance in plants　　　　　　　　　　　　　　　
  Tomohiro Uemura; Hyeran Kim; Chieko Saito; Kazuo Ebine; Takashi Ueda; Paul Schulze-Lefert; Akihiko Nakano
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：109, Number：5, First page：1784, Last page：1789, Jan. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1115146109
  DOI ID：10.1073/pnas.1115146109, ISSN：0027-8424, ORCID：50019880, SCOPUS ID：84863129073, Web of Science ID：WOS:000299731400085
• Endosomal trafficking pathway regulated by ARA6, a RAB5 GTPase unique to plants　　　　　　　　　　　　　　　
  Kazuo Ebine; Naoto Miyakawa; Masaru Fujimoto; Tomohiro Uemura; Akihiko Nakano; Takashi Ueda
  Small GTPases, Volume：3, Number：1, 2012, [Reviewed]
  Taylor and Francis Inc., English, Scientific journal
  DOI：https://doi.org/10.4161/sgtp.3.1.18299
  DOI ID：10.4161/sgtp.3.1.18299, ISSN：2154-1256, ORCID：50019900, PubMed ID：22710734, SCOPUS ID：84859092534
• Dynamic behavior of clathrin in Arabidopsis thaliana unveiled by live imaging　　　　　　　　　　　　　　　
  Emi Ito; Masaru Fujimoto; Kazuo Ebine; Tomohiro Uemura; Takashi Ueda; Akihiko Nakano
  PLANT JOURNAL, Volume：69, Number：2, First page：204, Last page：216, Jan. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2011.04782.x
  DOI ID：10.1111/j.1365-313X.2011.04782.x, ISSN：0960-7412, ORCID：50019892, SCOPUS ID：84855671917, Web of Science ID：WOS:000298874300002
• The occurrence of 'bulbs', a complex configuration of the vacuolar membrane, is affected by mutations of vacuolar SNARE and phospholipase in Arabidopsis　　　　　　　　　　　　　　　
  Chieko Saito; Tomohiro Uemura; Chie Awai; Motoki Tominaga; Kazuo Ebine; Jun Ito; Takashi Ueda; Hiroshi Abe; Miyo Terao Morita; Masao Tasaka; Akihiko Nakano
  PLANT JOURNAL, Volume：68, Number：1, First page：64, Last page：73, Oct. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2011.04665.x
  DOI ID：10.1111/j.1365-313X.2011.04665.x, ISSN：0960-7412, ORCID：50019883, SCOPUS ID：80053575143, Web of Science ID：WOS:000295395900006
• Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing　　　　　　　　　　　　　　　
  Iwai Ohbayashi; Mineko Konishi; Kazuo Ebine; Munetaka Sugiyama
  PLANT JOURNAL, Volume：67, Number：1, First page：49, Last page：60, Jul. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2011.04574.x
  DOI ID：10.1111/j.1365-313X.2011.04574.x, ISSN：0960-7412, eISSN：1365-313X, ORCID：50019894, SCOPUS ID：79959717714, Web of Science ID：WOS:000292104700005
• A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6　　　　　　　　　　　　　　　
  Kazuo Ebine; Masaru Fujimoto; Yusuke Okatani; Tomoaki Nishiyama; Tatsuaki Goh; Emi Ito; Tomoko Dainobu; Aiko Nishitani; Tomohiro Uemura; Masa H. Sato; Hans Thordal-Christensen; Nobuhiro Tsutsumi; Akihiko Nakano; Takashi Ueda
  NATURE CELL BIOLOGY, Volume：13, Number：7, First page：853, Last page：U279, Jul. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/ncb2270
  DOI ID：10.1038/ncb2270, ISSN：1465-7392, eISSN：1476-4679, ORCID：50019885, SCOPUS ID：79959939840, Web of Science ID：WOS:000292305700016
• Vacuolar/pre-vacuolar compartment Qa-SNAREs VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis　　　　　　　　　　　　　　　
  Tomohiro Uemura; Miyo Terao Morita; Kazuo Ebine; Yusuke Okatani; Daisuke Yano; Chieko Saito; Takashi Ueda; Akihiko Nakano
  PLANT JOURNAL, Volume：64, Number：5, First page：864, Last page：873, Dec. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-313X.2010.04372.x
  DOI ID：10.1111/j.1365-313X.2010.04372.x, ISSN：0960-7412, ORCID：50019907, SCOPUS ID：78649573452, Web of Science ID：WOS:000284592400013
• Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis　　　　　　　　　　　　　　　
  Yohann Boutte; Marcia Frescatada-Rosa; Shuzhen Men; Cheung-Ming Chow; Kazuo Ebine; Anna Gustavsson; Lenore Johansson; Takashi Ueda; Ian Moore; Gerd Juergens; Markus Grebe
  EMBO JOURNAL, Volume：29, Number：3, First page：546, Last page：558, Feb. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/emboj.2009.363
  DOI ID：10.1038/emboj.2009.363, ISSN：0261-4189, ORCID：50019893, SCOPUS ID：76349116068, Web of Science ID：WOS:000274233400004
• Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha　　　　　　　　　　　　　　　
  Atsuko Era; Motoki Tominaga; Kazuo Ebine; Chie Awai; Chieko Saito; Kimitsune Ishizaki; Katsuyuki T. Yamato; Takayuki Kohchi; Akihiko Nakano; Takashi Ueda
  PLANT AND CELL PHYSIOLOGY, Volume：50, Number：6, First page：1041, Last page：1048, Jun. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcp055
  DOI ID：10.1093/pcp/pcp055, ISSN：0032-0781, ORCID：50019888, SCOPUS ID：67649369329, Web of Science ID：WOS:000266967600004
• Unique mechanism of plant endocytic/vacuolar transport pathways　　　　　　　　　　　　　　　
  Kazuo Ebine; Takashi Ueda
  JOURNAL OF PLANT RESEARCH, Volume：122, Number：1, First page：21, Last page：30, Jan. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s10265-008-0200-x
  DOI ID：10.1007/s10265-008-0200-x, ISSN：0918-9440, ORCID：50019882, SCOPUS ID：58149333152, Web of Science ID：WOS:000262241300002
• Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions　　　　　　　　　　　　　　　
  Pankaj Dhonukshe; Hirokazu Tanaka; Tatsuaki Goh; Kazuo Ebine; Ari Pekka Mahonen; Kalika Prasad; Ikram Blilou; Niko Geldner; Jian Xu; Tomohiro Uemura; Joanne Chory; Takashi Ueda; Akihiko Nakano; Ben Scheres; Jiri Friml
  NATURE, Volume：456, Number：7224, First page：962, Last page：U75, Dec. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/nature07409
  DOI ID：10.1038/nature07409, ISSN：0028-0836, ORCID：50019895, SCOPUS ID：56349106623, Web of Science ID：WOS:000261768300048
• A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana　　　　　　　　　　　　　　　
  Kazuo Ebine; Yusuke Okatani; Tomohiro Uemura; Tatsuaki Goh; Keiko Shoda; Mitsuru Niihama; Miyo Terao Morita; Christoph Spitzer; Marisa S. Otegui; Akihiko Nakano; Takashi Ueda
  PLANT CELL, Volume：20, Number：11, First page：3006, Last page：3021, Nov. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.107.057711
  DOI ID：10.1105/tpc.107.057711, ISSN：1040-4651, ORCID：50019896, SCOPUS ID：58149332213, Web of Science ID：WOS:000262131500009
• VPS9a, the common activator for two distinct types of Rab5 GTPases, is essential for the development of Arabidopsis thaliana　　　　　　　　　　　　　　　
  Tatsuaki Goh; Wakana Uchida; Satoko Arakawa; Emi Ito; Tomoko Dainobu; Kazuo Ebine; Masaki Takeuchi; Ken Sato; Takashi Ueda; Akihiko Nakano
  PLANT CELL, Volume：19, Number：11, First page：3504, Last page：3515, Nov. 2007, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.107.053876
  DOI ID：10.1105/tpc.107.053876, ISSN：1040-4651, ORCID：50019898, SCOPUS ID：37849024683, Web of Science ID：WOS:000252268700016
• The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5　　　　　　　　　　　　　　　
  Thomas J. Haas; Marek K. Sliwinski; Dana E. Martinez; Mary Preuss; Kazuo Ebine; Takashi Ueda; Erik Nielsen; Greg Odorizzi; Marisa S. Otegui
  PLANT CELL, Volume：19, Number：4, First page：1295, Last page：1312, Apr. 2007, [Reviewed]
  English
  DOI：https://doi.org/10.1105/tpc.106.049346
  DOI ID：10.1105/tpc.106.049346, ISSN：1040-4651, ORCID：50019881, SCOPUS ID：34250643053, Web of Science ID：WOS:000246802200015
• Myxomycetes collected at the Tokiwamatsu Imperial Villa, Tokyo　　　　　　　　　　　　　　　
  Yamamoto Yukinori; Hagiwara Hiromitsu; Degawa Yousuke; Kawakami Shin-ichi; Inaba Shigeki; Kamono Akiko; Tanahashi Masahiko; Ebine Kazuo
  Miscellaneous reports of the National Park for Nature Study, Volume：36, First page：31, Last page：65, Mar. 2005
  Myxomycetes collected at the Tokiwamatsu Imperial Villa, Tokyo Pref., Japan were examined by field work and moist chamber culture. As a result, 56 taxa were enumerated. Among them 11 taxa (Clastoderma debaryanum van imperatorium, Dictydiaethalium ferrugineum, Licea kleistobolus, L pedicellata (Arcyria affinis), Diderma microsporum, Didymium chrysosporum, Physarum leucophaeum, P. melleum f. luteum, P. sulphureum and Macbrideola martini were new to Tokyo Pref. (Bonin Islands are excluded). As a total, 210 myxomycetes become known from Tokyo Pref, and 25 taxa are illustrated to clarify the concept of each taxon. Both lists of the myxomycetes found in Tokyo Pref. and the corticolous myxomycetes collected from living trees in this study are added.
  国立科学博物館, Japanese
  ISSN：0385-759X, CiNii Articles ID：110004709621, CiNii Books ID：AN00103849

• シロイヌナズナ根毛側面形成に関与する膜交通系の解明　　　　　　　　　　　　　　　
  佐藤雅彦; 海老根一生; 上田貴志; 檜垣匠; 中山隆宏; 紺野宏記; 今村寿子; 平野朋子
  Volume：87th, 2023
  J-Global ID：202302234121968143
• Analysis of PICALM2 and pollen-specific VAMP72 members in Arabidopsis　　　　　　　　　　　　　　　
  海老根一生; 海老根一生; 藤本優; 室啓太; 武内秀憲; 野澤彰; 澤崎達也; 東山哲也; 上田貴志; 上田貴志
  日本植物生理学会年会(Web), Volume：64th, 2023
  J-Global ID：202302248876760406
• コケ配偶体と花粉における生殖細胞分化の理解と制御に向けて　　　　　　　　　　　　　　　
  山岡尚平; 海老根一生; 海老根一生; 齊藤美咲; 樅木亮介; 吉竹良洋; 上田貴志; 上田貴志; 光田展隆; 西浜竜一; 西浜竜一; 荒木崇; 河内孝之
  Volume：86th, 2022
  J-Global ID：202302230962734914
• SYP123-VAMP727 is involved in the secretion of the inner cell wall components to the root hair shank in Arabidopsis　　　　　　　　　　　　　　　
  佐藤雅彦; 海老根一生; 上田貴志; 檜垣匠; 紺野宏記; 中山隆宏; 平野朋子
  日本植物生理学会年会(Web), Volume：63rd, 2022
  J-Global ID：202202216741771060
• A bHLH transcription factor complex required for land plant germ cell differentiation　　　　　　　　　　　　　　　
  山岡尚平; 齊藤美咲; 樅木亮介; 吉竹良洋; 光田展隆; 海老根一生; 海老根一生; 西浜竜一; 西浜竜一; 荒木崇; 河内孝之
  日本農芸化学会大会講演要旨集(Web), Volume：2022, 2022
  ISSN：2186-7976, J-Global ID：202202210808349888
• Analysis of GH17 members regulating generative cell engulfment by the vegetative cell in Arabidopsis　　　　　　　　　　　　　　　
  海老根一生; 海老根一生; 山岡尚平; 上田貴志; 上田貴志
  日本植物生理学会年会(Web), Volume：62nd, 2021
  J-Global ID：202102282523407154
• シロイヌナズナの雄原細胞形成に関わるGH17メンバーの解析　　　　　　　　　　　　　　　
  海老根一生; 海老根一生; 山岡尚平; 上田貴志; 上田貴志
  Volume：84th, 2020
  J-Global ID：202302278743534156
• Analysis of GH17 members regulating engulfment of the generative cell into the vegetative cell in Arabidopsis　　　　　　　　　　　　　　　
  海老根一生; 海老根一生; 山岡尚平; 上田貴志; 上田貴志
  日本植物生理学会年会(Web), Volume：61st, 2020
  J-Global ID：202002220586226291
• シロイヌナズナにおけるHOPS/CORVET complexの解析
  竹元廣大; 海老根一生; 郷達明; 井藤純; 中野明彦; 中野明彦; 上田貴志
  日本植物学会大会研究発表記録, Volume：79th, First page：163, 01 Sep. 2015
  Japanese
  J-Global ID：201502219286823388
• 学部生に伝える研究最前線　　　　　　　　　　　　　　　
  海老根 一生; 上田 貴志; 牧島 一夫; 中澤 知洋; 寺坂 尚絃; 菅 裕明
  Volume：46, Number：3, First page：7, Last page：9, Sep. 2014
  植物の液胞へ物質を運ぶ仕組みを解明／宇宙最強の磁石天体は、磁力で変形していた／1つの遺伝子から2つのペプチドを同時に合成
  Japanese
  CiNii Articles ID：120005477796, CiNii Books ID：AA11719620
• Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions (vol 456, pg 962, 2008)　　　　　　　　　　　　　　　
  Pankaj Dhonukshe; Hirokazu Tanaka; Tatsuaki Goh; Kazuo Ebine; Ari Pekka Maehoenen; Kalika Prasad; Ikram Blilou; Niko Geldner; Jian Xu; Tomohiro Uemura; Joanne Chory; Takashi Ueda; Akihiko Nakano; Ben Scheres; Jiri Friml
  NATURE, Volume：511, Number：7509, Jul. 2014
  English, Others
  DOI：https://doi.org/10.1038/nature13549
  DOI ID：10.1038/nature13549, ISSN：0028-0836, eISSN：1476-4687, Web of Science ID：WOS:000338992200043
• 植物の液胞輸送経路の解析
  海老根一生; 井上丈司; 井藤純; 伊藤瑛海; 植村知博; 郷達明; 安部弘; 佐藤健; 中野明彦; 上田貴志
  日本植物生理学会年会要旨集, Volume：55th, First page：210, 11 Mar. 2014
  Japanese
  J-Global ID：201402294643094628
• ゼニゴケを用いた植物特異的膜交通経路の研究
  恵良美子; 海老根一生; 石崎公庸; 白川一; 西浜竜一; 河内孝之; 中野明彦; 上田貴志
  Volume：77th, First page：140, 20 Aug. 2013
  Japanese
  J-Global ID：201302256106492459
• 液胞膜の融合を制御する2つのSNARE複合体の解析
  海老根一生; 藤本優; 郷達明; 井藤純; 齋藤知恵子; 植村知博; 中野明彦; 上田貴志
  日本植物学会大会研究発表記録, Volume：75th, First page：136, 10 Sep. 2011
  Japanese
  J-Global ID：201102253421274334
• 研究ニュース　　　　　　　　　　　　　　　
  丸山 真一朗; 野崎 久義; 井出 哲; 吉岡 孝高; 五神 真; 海老根 一生; 上田 貴志; 若林 憲一
  Volume：43, Number：3, First page：10, Last page：14, Sep. 2011
  ミドリムシの祖先は「アカムシ」だった？／東北沖巨大地震はどのような現象だったのか？／励起子ボース・アインシュタイン凝縮の実現／細胞内の交通網はどのように整備されたのか／緑藻の光の好き嫌いを決める細胞内シグナル
  Japanese
  CiNii Articles ID：120003367893, CiNii Books ID：AA11719620
• エンドソーム/液胞膜の融合を制御するR‐SNAREの解析
  海老根一生; 藤本優; 郷達明; 井藤純; 植村知博; 中野明彦; 上田貴志
  日本植物学会大会研究発表記録, Volume：73rd, First page：143, 17 Sep. 2009
  Japanese
  J-Global ID：200902285989713112
• 種子植物特異的R‐SNAREであるVAMP727の機能解析　　　　　　　　　　　　　　　
  海老根一生; 岡谷祐哉; 郷達明; 井藤純; 植村知博; 中野明彦; 上田貴志
  日本植物生理学会年会要旨集, Volume：50th, First page：317, 16 Mar. 2009
  Japanese
  DOI：https://doi.org/10.14841/jspp.2009.0.0811.0
  DOI ID：10.14841/jspp.2009.0.0811.0, J-Global ID：200902298415758368
• 植物のRab5メンバーの動態と活性化制御機構の解析　　　　　　　　　　　　　　　
  郷 達明; 海老根 一生; 植村 知博; 上田 貴志; 中野 明彦
  Volume：16, Number：2, First page：169, Last page：170, 05 Oct. 2007
  Japanese
  ISSN：1342-2634, CiNii Articles ID：10019824105, CiNii Books ID：AN1055579X
• Functional analysis of AtVamp727 in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Yusuke Okatani; Kazuo Ebine; Tomoko Dainobu; Tatsuaki Goh; Tomohiro Uemura; Akihiko Nakano; Takashi Ueda
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S205, Last page：S205, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922701298
• Endocytosis regulates flowering of Arabidopsis thaliana　　　　　　　　　　　　　　　
  Kazuo Ebine; Akihiko Nakano; Takashi Ueda
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S114, Last page：S114, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922700453
• Endocytosis regulates flowering of Arabidopsis thaliana　　　　　　　　　　　　　　　
  EBINE Kazuo; NAKANO Akihiko; UEDA Takashi
  Volume：119, First page：141, Last page：141, 01 Dec. 2006
  English
  ISSN：0918-9440, CiNii Articles ID：10019309816, CiNii Books ID：AA10900218

• The Japanese Society of Plant Physiologists
• The Botanical Society of Japan

• Analysis of plant vacuolar trasnport system regulated by RAB5　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2021 - 31 Mar. 2024
  National Institute for Basic Biology
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  Grant number：21K06222
  論文ID：50191572
• 様々なタイプのオートファジーによる植物の高次機能発現　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 28 Jun. 2019 - 31 Mar. 2024
  Meiji University
  Grant amount(Total)：66820000, Direct funding：51400000, Indirect funding：15420000
  Grant number：19H05713
• Analysis of membrane trafficking mechanisms regulating male gametogenesis in plant　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 01 Apr. 2019 - 31 Mar. 2021
  National Institute for Basic Biology
  Grant amount(Total)：8580000, Direct funding：6600000, Indirect funding：1980000
  Grant number：19H04872
• Analysis of vacuolar transport mechanisms in plant cells　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), 01 Apr. 2018 - 31 Mar. 2021
  Ebine Kazuo, National Institute for Basic Biology
  Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
  The vacuole is the largest and multifunctional organelle in plant cells, and many proteins are transported to and stored in this organelle through membrane traffic pathways. We analyzed detail molecular mechanisms of transport pathways to the vacuole, especially focus on plant-unique vacuolar transport pathway. We found that at least two plant-unique vacuolar transport pathways exist in plant cells, and one of this pathway contributes to the stress response of plant.
  Grant number：18K06303
• Analysis of membrane trafficking mechanisms regulating gametophyte formation in plant　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 01 Apr. 2017 - 31 Mar. 2019
  National Institute for Basic Biology
  Grant amount(Total)：9360000, Direct funding：7200000, Indirect funding：2160000
  Grant number：17H05850
• Analysis of molecular mechanisms of vacuolar membrane fusion regulated by SNARE proteins in plant cells　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), 01 Apr. 2015 - 31 Mar. 2017
  EBINE Kazuo
  Grant amount(Total)：4160000, Direct funding：3200000, Indirect funding：960000
  The multifunctional vacuole is the largest organelle in plant cells, to and in which many proteins and other components are transported and stored. Three vacuolar transport pathways exist in Arabidopsis cells, but detail mechanisms of these pathways are still unclear. The analysis of transport mechanism of VHP1 to the vacuole suggests that RAB5-dependent RAB7-AP3-independent pathway, which is one of the plant unique pathways, is related to the transport pathway from ER to the vacuole. SNARE proteins are conserved key molecules regulating membrane fusion in membrane trafficking pathways. Vacuolar transport also involves a set of SNARE proteins, which mainly reside on the vacuolar membrane. Vacuolar SNAREs have also been shown to be required for vacuolar morphogenesis. VAM3/SYP22/SGR3 is a Qa-SNARE localizing on the vacuolar membrane. By the analysis of sgr3-1 mutant, alpha-SNAP was isolated as a novel regulator of vacuolar dynamics in plant.
  Grant number：15K18551
• Functions and molecular mechanisms of membrane trafficking in biogenesis and functions of cell wall　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 28 Jun. 2012 - 31 Mar. 2017
  UEDA Takashi; UEMURA Tomohiro; FUJIMOTO Masaru; EBINE Kazuo
  Grant amount(Total)：47190000, Direct funding：36300000, Indirect funding：10890000
  We analyzed mechanisms of cell wall construction and cell wall functions with various techniques, including super-resolution microscopy, biochemistry, and genetics. We succeeded in fine-resolution imaging of transport of the cellulose synthase complex, which is distinctly dependent on PI3K and PI4K. We also analyzed RABA/RAB11 functions in endocytosis of FLS2 to demonstrate functional diversification of RABA/RAB11 members. Furthermore, we demonstrated that the plant vacuolar transport pathway is distinctly regulated from yeasts and animals, which plays a pivotal role in cell wall integrity. Evolutionary cell biological analyses of the membrane trafficking system using the liverwort, Marchantia polymorpha, is also currently underway.
  Grant number：24114003
• アピコンプレクサＲａｂ５Ｂと陸上植物ＡＲＡ６が制御する小胞輸送経路の解析　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, Grant-in-Aid for JSPS Fellows, 2011 - 31 Mar. 2014
  National Institute of Infectious Diseases
  Grant amount(Total)：2400000, Direct funding：2400000
  Grant number：11J08803
• エンドサイトーシスによる植物の高次形態形成制御の解析　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, Grant-in-Aid for JSPS Fellows, 2007 - 2009
  The University of Tokyo
  Grant amount(Total)：2700000, Direct funding：2700000
  Grant number：07J05010