Performance information

• Biosynthesis of unnatural polyisoprenes by engineered prenyltransferases on rubber particles
  Seiji Takahashi; Miki Suenaga-Hiromori; Tomoki Ishii; Nadia Nur Shazana Binti Abu Talib Khan; Tomoyo Mikami; Tomohiro Takahashi; Chiho Minakawa; Fumihiro Yanbe; Toshiyuki Waki; Toru Nakayama; Riki Imaizumi; Taro Yanai; Kunishige Kataoka; Satoshi Yamashita; Kohei Takeshita; Hiroaki Matsuura; Naoki Sakai; Masaki Yamamoto; Haruhiko Yamaguchi; Yukino Miyagi-Inoue; Kazuhisa Fushihara; Yuzuru Tozawa
  Jul. 2024, [Reviewed]
  Abstract

  Natural rubber (NR) is a sustainable biopolymer consisting mainly of cis-1,4-polyisoprene. Modifying an NR biosynthetic enzyme is a promising strategy to bioproduce novel polymers. Here, we have elucidated the NR biosynthetic mechanism and successfully developed novel enzymes that synthesise NR-sized polyisoprenes with unnatural substrates. NR is synthesised by a cis-prenyltransferase (cPT) on rubber particles (RPs), NR-harbouring lipid monolayer membrane organelles. However, the key to NR biosynthesis is not specialised cPTs, but the proper arrangement of cPTs on RPs since cPTs from various non-NR-producing organisms, such as humans, synthesise NR when introduced into the RPs. A tomato cPT, which condenses only one isoprene unit, was engineered to synthesise novel NR-sized polyisoprenes with artificial substrates by modifying residues for product size determination. Furthermore, the introduction of a modified trans-prenyltransferase into RPs led to the synthesis of NR-sized trans-1,4-polyisoprenes. This RP system could be used as a versatile platform for enzymatic polyisoprenoid synthesis.

  
  Springer Science and Business Media LLC
  DOI：https://doi.org/10.21203/rs.3.rs-3615345/v1
  DOI ID：10.21203/rs.3.rs-3615345/v1
• Partner-switching components PmgA and Ssr1600 regulate high-light acclimation in Synechocystis sp. PCC 6803
  Riku Nakamura; Yuji Takahashi; Shogo Tachibana; Arisa Terada; Kakeru Suzuki; Kumika Kondo; Yuzuru Tozawa; Yukako Hihara
  Plant Physiology, Volume：196, Number：1, First page：621, Last page：633, Jun. 2024, [Reviewed]
  Abstract
  
  Photomixotrophic growth A (PmgA) is a pleiotropic regulator essential for growth under photomixotrophic and prolonged high-light (HL) conditions in the cyanobacterium Synechocystis sp. PCC 6803. The overall similarity with the antisigma factor of the bacterial partner-switching system indicates that PmgA exerts a regulatory function via phosphorylation of its target proteins. In this study, we performed an in vitro phosphorylation assay and protein–protein interaction analysis and found that PmgA interacts with 4 antisigma antagonist homologs, Ssr1600, Slr1856, Slr1859, and Slr1912, but specifically phosphorylates Ssr1600. Phenotypic analyses using the set of gene disruption and overexpression strains of pmgA and ssr1600 revealed that phosphorylation by PmgA is essential for the accumulation of Ssr1600 protein in vivo. The ssr1600-disrupted mutant showed similar phenotypes as those previously reported for the pmgA-disrupted mutant, namely, no obvious phenotype just after the shift to HL, but higher chlorophyll content, 5-aminolevulinic acid synthesis activity, and psaAB transcript levels than those in the wild type after 6 h. These findings indicate that the phosphorylated form of Ssr1600 works as the output of the partner-switching system to coordinately repress chlorophyll biosynthesis and accumulation of photosystem I during HL acclimation.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiae323
  DOI ID：10.1093/plphys/kiae323, ISSN：0032-0889, eISSN：1532-2548
• Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins.　　　　　　　　　　　　　　　
  Fu Kuroiwa; Hiraku Suda; Maho Yabuki; Kimie Atsuzawa; Haruhiko Yamaguchi; Masatsugu Toyota; Yasuko Kaneko; Satoshi Yamashita; Seiji Takahashi; Yuzuru Tozawa
  Bioscience, biotechnology, and biochemistry, Mar. 2024, [Reviewed], [Corresponding], ［International magazine］
  Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.
  English, Scientific journal
  DOI：https://doi.org/10.1093/bbb/zbae026
  DOI ID：10.1093/bbb/zbae026, PubMed ID：38444196
• Assembly of Cell-Free Synthesized Ion Channel Molecules in Artificial Lipid Bilayer Observed by Atomic Force Microscopy　　　　　　　　　　　　　　　
  Goh, Melvin Wei Shern; Tozawa, Yuzuru; Tero, Ryugo
  Membranes, Volume：13, Number：11, First page：854, Last page：854, Oct. 2023, [Reviewed], [Invited], ［International magazine］
  English, Scientific journal
  DOI：https://doi.org/10.3390/membranes13110854
  DOI ID：10.3390/membranes13110854
• Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule.　　　　　　　　　　　　　　　
  Fu Kuroiwa; Akira Nishino; Yasuko Mandal; Masataka Honzawa; Miki Suenaga-Hiromori; Kakeru Suzuki; Yukie Takani; Yukino Miyagi-Inoue; Haruhiko Yamaguchi; Satoshi Yamashita; Seiji Takahashi; Yuzuru Tozawa
  Scientific reports, Volume：12, Number：1, First page：3734, Last page：3734, Mar. 2022, [Reviewed], [Last, Corresponding], ［International magazine］
  Natural rubber of the Para rubber tree (Hevea brasiliensis) is synthesized as a result of prenyltransferase activity. The proteins HRT1, HRT2, and HRBP have been identified as candidate components of the rubber biosynthetic machinery. To clarify the contribution of these proteins to prenyltransferase activity, we established a cell-free translation system for nanodisc-based protein reconstitution and measured the enzyme activity of the protein-nanodisc complexes. Co-expression of HRT1 and HRBP in the presence of nanodiscs yielded marked polyisoprene synthesis activity. By contrast, neither HRT1, HRT2, or HRBP alone nor a complex of HRT2 and HRBP manifested such activity. Similar analysis of guayule (Parthenium argentatum) proteins revealed that three HRT1 homologs (PaCPT1-3) manifested prenyltransferase activity only when co-expressed with PaCBP, the homolog of HRBP. Our results thus indicate that two heterologous subunits form the core prenyltransferase of the rubber biosynthetic machinery. A recently developed structure modeling program predicted the structure of such heterodimer complexes including HRT1/HRBP and PaCPT2/PaCBP. HRT and PaCPT proteins were also found to possess affinity for a lipid membrane in the absence of HRBP or PaCBP, and structure modeling implicated an amphipathic α-helical domain of HRT1 and PaCPT2 in membrane binding of these proteins.
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41598-022-07564-y
  DOI ID：10.1038/s41598-022-07564-y, PubMed ID：35260628, PubMed Central ID：PMC8904820
• Catalytic promiscuity of rice 2-oxoglutarate/Fe(II)-dependent dioxygenases supports xenobiotic metabolism　　　　　　　　　　　　　　　
  Natsuki Takamura; Akihiko Yamazaki; Nozomi Sakuma; Sakiko Hirose; Motonari Sakai; Yukie Takani; Satoshi Yamashita; Masahiro Oshima; Makoto Kuroki; Yuzuru Tozawa
  Plant Physiology, Volume：187, Number：2, First page：816, Last page：828, Jun. 2021, [Reviewed], [Corresponding], ［International magazine］
  
  The rice (Oryza sativa) 2-oxoglutarate (2OG)/Fe(II)-dependent dioxygenase HIS1 mediates the catalytic inactivation of five distinct β-triketone herbicides (bTHs). By assessing the effects of plant growth regulators on HIS1 enzyme function, we found that HIS1 mediates the hydroxylation of trinexapac-ethyl (TE) in the presence of Fe2+ and 2OG. TE blocks gibberellin biosynthesis, and we observed that its addition to culture medium induced growth retardation of rice seedlings in a concentration-dependent manner. Similar treatment with hydroxylated TE revealed that hydroxylation greatly attenuated the inhibitory effect of TE on plant growth. Forced expression of HIS1 in a rice his1 mutant also reduced its sensitivity to TE compared with that of the nontransformant. These results indicate that HIS1 metabolizes TE and thereby markedly reduces its ability to slow plant growth. Furthermore, analysis of five rice HIS1-like (HSL) proteins revealed that OsHSL2 and OsHSL4 also metabolize TE in vitro. HSLs from wheat (Triticum aestivum) and barley (Hordeum vulgare) also showed such activity. In contrast, OsHSL1, which shares the highest amino acid sequence identity with HIS1 and metabolizes the bTH tefuryltrione, did not manifest TE-metabolizing activity. Site-directed mutagenesis of OsHSL1 informed by structural models showed that substitution of three amino acids with the corresponding residues of HIS1 conferred TE-metabolizing activity similar to that of HIS1. Our results thus reveal a catalytic promiscuity of HIS1 and its related enzymes that support xenobiotic metabolism in plants.
  Oxford University Press (OUP), English, Scientific journal
  DOI：https://doi.org/10.1093/plphys/kiab293
  DOI ID：10.1093/plphys/kiab293, eISSN：1532-2548, PubMed ID：34608958, PubMed Central ID：PMC8491036
• Characterization of Plasmodium falciparum Pantothenate Kinase and Identification of Its Inhibitors From Natural Products
  Arif Nurkanto; Ghulam Jeelani; Herbert J. Santos; Yulia Rahmawati; Mihoko Mori; Yumi Nakamura; Kana Goto; Yoko Saikawa; Takeshi Annoura; Yuzuru Tozawa; Takaya Sakura; Daniel Ken Inaoka; Kazuro Shiomi; Tomoyoshi Nozaki
  Frontiers in Cellular and Infection Microbiology, Volume：11, Mar. 2021, [Reviewed]
  Coenzyme A (CoA) is a well-known cofactor that plays an essential role in many metabolic reactions in all organisms. In Plasmodium falciparum, the most deadly among Plasmodium species that cause malaria, CoA and its biosynthetic pathway have been proven to be indispensable. The first and rate-limiting reaction in the CoA biosynthetic pathway is catalyzed by two putative pantothenate kinases (PfPanK1 and 2) in this parasite. Here we produced, purified, and biochemically characterized recombinant PfPanK1 for the first time. PfPanK1 showed activity using pantetheine besides pantothenate, as the primary substrate, indicating that CoA biosynthesis in the blood stage of P. falciparum can bypass pantothenate. We further developed a robust and reliable screening system to identify inhibitors using recombinant PfPanK1 and identified four PfPanK inhibitors from natural compounds.
  Frontiers Media SA, Scientific journal
  DOI：https://doi.org/10.3389/fcimb.2021.639065
  DOI ID：10.3389/fcimb.2021.639065, eISSN：2235-2988
• Lateral voltage as a new input for artificial lipid bilayer systems
  Teng Ma; Madoka Sato; Maki Komiya; Kensaku Kanomata; Takaya Watanabe; Xingyao Feng; Ryusuke Miyata; Daisuke Tadaki; Fumihiko Hirose; Yuzuru Tozawa; Ayumi Hirano-Iwata
  Faraday Discussions, Volume：233, First page：244, Last page：256, 2021, [Reviewed], [Invited]
  In this work, we propose lateral voltage as a new input for use in artificial lipid bilayer systems in addition to the commonly used transmembrane voltage. To apply a lateral...
  Royal Society of Chemistry (RSC), English, Scientific journal
  DOI：https://doi.org/10.1039/d1fd00045d
  DOI ID：10.1039/d1fd00045d, ISSN：1359-6640, eISSN：1364-5498
• Establishment of a cell-free translation system from rice callus extracts
  Kakeru Suzuki; Haruka Inoue; Satoshi Matsuoka; Ryugo Tero; Ayumi Hirano-Iwata; Yuzuru Tozawa
  Bioscience, Biotechnology, and Biochemistry, Volume：84, Number：10, First page：2028, Last page：2036, Oct. 2020, [Last, Corresponding]
  
  Eukaryotic in vitro translation systems require large numbers of protein and RNA components and thereby rely on the use of cell extracts. Here we established a new in vitro translation system based on rice callus extract (RCE). We confirmed that RCE maintains its initial activity even after five freeze-thaw cycles and that the optimum temperature for translation is around 20°C. We demonstrated that the RCE system allows the synthesis of hERG, a large membrane protein, in the presence of liposomes. We also showed that the introduction of a bicistronic mRNA based on 2A peptide to RCE allowed the production of two distinct proteins from a single mRNA. Our new method thus facilitates laboratory-scale production of cell extracts, making it a useful tool for the in vitro synthesis of proteins for biochemical studies.
  Oxford University Press (OUP), Scientific journal
  DOI：https://doi.org/10.1080/09168451.2020.1779024
  DOI ID：10.1080/09168451.2020.1779024, ISSN：0916-8451, eISSN：1347-6947
• Characterization of mitochondrial carrier proteins of malaria parasite Plasmodium falciparum based on in vitro translation and reconstitution.　　　　　　　　　　　　　　　
  Akira Nozawa; Daisuke Ito; Mohamed Ibrahim; Herbert J Santos; Takafumi Tsuboi; Yuzuru Tozawa
  Parasitology international, Volume：79, First page：102160, Last page：102160, Jun. 2020, [Reviewed], [Corresponding], ［International magazine］
  Members of the mitochondrial carrier (MC) family of membrane transporters play important roles in cellular metabolism. We previously established an in vitro reconstitution system for membrane transporters based on wheat germ cell-free translation system. We have now applied this reconstitution system to the comparative analysis of MC proteins from the malaria parasite Plasmodium falciparum and Saccharomyces cerevisiae. We synthesized twelve putative P. falciparum MCs and determined the transport activities of four of these proteins including PF3D7_1037300 protein (ADP/ATP translocator), PF3D7_1004800 protein (ADP/ATP translocator), PF3D7_1202200 protein (phosphate carrier), and PF3D7_1241600 protein (S-adenosylmethionine transporter). In addition, we tested the effect of cardiolipin on the activity of MC proteins. The transport activities of the yeast MCs, ScAac2p, ScGgc1p, ScDic1p, ScPic1p, and ScSam5p, which localize to the mitochondrial inner membrane, were increased by cardiolipin supplementation, whereas that of ScAnt1p, which localizes to the peroxisome membrane, was not significantly affected. Together, this indicates that the functional properties of the reconstituted MCs reflect the lipid content of their native membranes. Except for PF3D7_1241600 protein, these P. falciparum proteins manifested cardiolipin-dependent transport activities. Immunofluorescence analysis showed that PF3D7_1241600 protein is not mainly localized to the mitochondria of P. falciparum cells. We thus revealed the functions of four MC proteins of the malaria parasite and the effects of cardiolipin on their activities.
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.parint.2020.102160
  DOI ID：10.1016/j.parint.2020.102160, PubMed ID：32574727
• Advances in artificial cell membrane systems as a platform for reconstituting ion channels
  Maki Komiya; Miki Kato; Daisuke Tadaki; Teng Ma; Hideaki Yamamoto; Ryugo Tero; Yuzuru Tozawa; Michio Niwano
  The Chemical Record, Volume：accepted, Number：7, First page：730, Last page：742, 2020, [Reviewed]
  Wiley, English, Scientific journal
  DOI：https://doi.org/10.1002/tcr.201900094
  DOI ID：10.1002/tcr.201900094, ISSN：1527-8999, eISSN：1528-0691
• Substrate specificity of plastid phosphate transporters in a non-photosynthetic diatom and its implication in evolution of red alga-derived complex plastids　　　　　　　　　　　　　　　
  Daniel Moog; Akira Nozawa; Yuzuru Tozawa; Ryoma Kamikawa
  Volume：10, Jan. 2020, [Reviewed]
  English, Scientific journal
• Novel lineage-specific transmembrane β-barrel proteins in the endoplasmic reticulum of Entamoeba histolytica.　　　　　　　　　　　　　　　
  Herbert J Santos; Kenichiro Imai; Takashi Makiuchi; Kentaro Tomii; Paul Horton; Akira Nozawa; Kenta Okada; Yuzuru Tozawa; Tomoyoshi Nozaki
  The FEBS journal, Volume：286, Number：17, First page：3416, Last page：3432, Sep. 2019, [Reviewed], ［International magazine］
  β-barrel outer membrane proteins (BOMPs) are essential components of outer membranes of Gram-negative bacteria and endosymbiotic organelles, usually involved in the transport of proteins and substrates across the membrane. Based on the analysis of our in silico BOMP predictor data for the Entamoeba histolytica genome, we detected a new transmembrane β-barrel domain-containing protein, EHI_192610. Sequence analysis revealed that this protein is unique to Entamoeba species, and it exclusively clusters with a homolog, EHI_099780, which is similarly lineage specific. Both proteins possess an N-terminal signal peptide sequence as well as multiple repeats that contain dyad hydrophobic periodicities. Data from immunofluorescence assay of trophozoites expressing the respective candidates showed the absence of colocalization with mitosomal marker, and interestingly demonstrated partial colocalization with endoplasmic reticulum (ER) proteins instead. Integration to organellar membrane was supported by carbonate fractionation assay and immunoelectron microscopy. CD analysis of reconstituted proteoliposomes containing EHI_192610 showed a spectrum demonstrating a predominant β-sheet structure, suggesting that this protein is β-strand rich. Furthermore, the presence of repeat regions with predicted transmembrane β-strand pairs in both EHI_192610 and EHI_099780, is consistent with the hypothesis that BOMPs originated from the amplification of ββ-hairpin modules, suggesting that the two Entamoeba-specific proteins are novel β-barrels, intriguingly localized partially to the ER membrane.
  English, Scientific journal
  DOI：https://doi.org/10.1111/febs.14870
  DOI ID：10.1111/febs.14870, ISSN：1742-464X, PubMed ID：31045303
• A Teflon-based system for applying multidirectional voltages to lipid bilayers as a novel platform for membrane proteins
  Maki Komiya; Kensaku Kanomata; Ryo Yokota; Yusuke Tsuneta; Madoka Sato; Daichi Yamaura; Daisuke Tadaki; Teng Ma; Hideaki Yamamoto; Yuzuru Tozawa; Albert Marti; Jordi Madrenas; Shigeru Kubota; Fumihiko Hirose; Michio Niwano; Ayumi Hirano-Iwata
  Jul. 2019
  Artificial bilayer lipid membranes (BLMs), along with patch-clamped
  
  membranes, are frequently used for functional analyses of membrane proteins. In
  
  both methods, the electric properties of membranes are characterized by only
  
  one parameter, namely, transmembrane potential. Here the construction of a
  
  novel BLM system was reported, in which membrane voltages can be controlled in
  
  a lateral direction in addition to conventional transmembrane direction. A
  
  microaperture was fabricated in a Teflon film and Ti electrodes were evaporated
  
  around the aperture. BLMs were reproducibly formed in the aperture without
  
  being affected by the presence of the electrodes. The application of a lateral
  
  voltage induced no significant changes in the electric properties of the BLMs,
  
  such as baseline current, transmembrane resistance, and transmembrane
  
  capacitance. In contrast, lateral voltages clearly affected the activities of
  
  biological ion channels, suggesting that the lateral voltage might be a useful
  
  parameter for analyzing channel activities. The present Teflon-based system in
  
  which multidirectional voltages can be applied to BLMs represent a promising
  
  platform for the analysis of underlying functional properties of membrane
  
  proteins.
  Scientific journal
  arXiv ID：arXiv:1907.05643
• A rice gene that confers broad-spectrum resistance to β-triketone herbicides.　　　　　　　　　　　　　　　
  Maeda H; Murata K; Sakuma N; Takei S; Yamazaki A; Karim MR; Kawata M; Hirose S; Kawagishi-Kobayashi M; Taniguchi Y; Suzuki S; Sekino K; Ohshima M; Kato H; Yoshida H; Tozawa Y
  Science (New York, N.Y.), Volume：365, Number：6451, First page：393, Last page：396, Jul. 2019, [Reviewed], [Last, Corresponding], ［International magazine］
  The genetic variation of rice cultivars provides a resource for further varietal improvement through breeding. Some rice varieties are sensitive to benzobicyclon (BBC), a β-triketone herbicide that inhibits 4-hydroxyphenylpyruvate dioxygenase (HPPD). Here we identify a rice gene, HIS1 (HPPD INHIBITOR SENSITIVE 1), that confers resistance to BBC and other β-triketone herbicides. We show that HIS1 encodes an Fe(II)/2-oxoglutarate-dependent oxygenase that detoxifies β-triketone herbicides by catalyzing their hydroxylation. Genealogy analysis revealed that BBC-sensitive rice variants inherited a dysfunctional his1 allele from an indica rice variety. Forced expression of HIS1 in Arabidopsis conferred resistance not only to BBC but also to four additional β-triketone herbicides. HIS1 may prove useful for breeding herbicide-resistant crops.
  English, Scientific journal
  DOI：https://doi.org/10.1126/science.aax0379
  DOI ID：10.1126/science.aax0379, ISSN：0036-8075, PubMed ID：31346065
• N-myristoylation and S-acylation are common modifications of Ca2+-regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel　　　　　　　　　　　　　　　
  Shunya Saito; Shin Hamamoto; Koko Moriya; Aiko Matsuura; Yoko Sato; Jun Muto; Hiroto Noguchi; Seiji Yamauchi; Yuzuru Tozawa; Minoru Ueda; Kenji Hashimoto; Philipp Köster; Qiuyan Dong; Katrin Held; Jörg Kudla; Toshihiko Utsumi; Nobuyuki Uozumi
  New Phytologist, Volume：218, Number：4, First page：1504, Last page：1521, Jun. 2018, [Reviewed]
  Blackwell Publishing Ltd, English, Scientific journal
  DOI：https://doi.org/10.1111/nph.15053
  DOI ID：10.1111/nph.15053, ISSN：1469-8137, PubMed ID：29498046, SCOPUS ID：85042622802
• The checkpoint kinase TOR (target of rapamycin) regulates expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) and modulates chloroplast ribosomal RNA synthesis in a unicellular red alga　　　　　　　　　　　　　　　
  Sousuke Imamura; Yuhta Nomura; Tokiaki Takemura; Imran Pancha; Keiko Taki; Kazuki Toguchi; Yuzuru Tozawa; Kan Tanaka
  Plant Journal, Volume：94, Number：2, First page：327, Last page：339, Apr. 2018, [Reviewed]
  Blackwell Publishing Ltd, English, Scientific journal
  DOI：https://doi.org/10.1111/tpj.13859
  DOI ID：10.1111/tpj.13859, ISSN：1365-313X, PubMed ID：29441718, SCOPUS ID：85044217434
• Quantification of Drug Side Effects on Cell-Free Synthesized Ion-Channels Reconstituted in Bilayer Lipid Membranes　　　　　　　　　　　　　　　
  Kato Miki; Hirano Ayumi; Inoue Haruka; Yamaura Daichi; Yokota Reo; Komiya Maki; Tadaki Daisuke; Yamamoto Hidaka; Tozawa Yuzuru; Niwano Michio
  Abstract of annual meeting of the Surface Science of Japan, Volume：2018, Number：0, First page：181, Last page：181, 2018
  We aim to develop a drug screening system based on a reconstitution system in which ion channels are embedded in an artificial lipid bilayer membrane (BLM). We have been developing stable BLMs based on silicon (Si) microfabrication. We previously reported on efficient incorporation of ion channels in BLMs. Here we applied this system to a cell-free synthesized hERG channel and tried to quantify drug side effects on this channel.
  The Japan Society of Vacuum and Surface Science, Japanese
  DOI：https://doi.org/10.14886/sssj2008.2018.0_181
  DOI ID：10.14886/sssj2008.2018.0_181, CiNii Articles ID：130007519109
• Mechanically stable solvent-free lipid bilayers in nano- and micro-tapered apertures for reconstitution of cell-free synthesized hERG channels　　　　　　　　　　　　　　　
  Daisuke Tadaki; Daichi Yamaura; Shun Araki; Miyu Yoshida; Kohei Arata; Takeshi Ohori; Ken-ichi Ishibashi; Miki Kato; Teng Ma; Ryusuke Miyata; Yuzuru Tozawa; Hideaki Yamamoto; Michio Niwano; Ayumi Hirano-Iwata
  SCIENTIFIC REPORTS, Volume：7, Number：1, First page：17736, Dec. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/s41598-017-17905-x
  DOI ID：10.1038/s41598-017-17905-x, ISSN：2045-2322, PubMed ID：29255199, Web of Science ID：WOS:000418323900048
• Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor　　　　　　　　　　　　　　　
  Jelena Beljantseva; Pavel Kudrin; Steffi Jimmy; Marcel Ehn; Radek Pohl; Vallo Varik; Yuzuru Tozawa; Victoria Shingler; Tanel Tenson; Dominik Rejman; Vasili Hauryliuk
  SCIENTIFIC REPORTS, Volume：7, First page：41839, Feb. 2017, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep41839
  DOI ID：10.1038/srep41839, ISSN：2045-2322, PubMed ID：28157202, Web of Science ID：WOS:000393552300001
• Auxotrophy-based High Throughput Screening assay for the identification of Bacillus subtilis stringent response inhibitors　　　　　　　　　　　　　　　
  Liis Andresen; Vallo Varik; Yuzuru Tozawa; Steffi Jimmy; Stina Lindberg; Tanel Tenson; Vasili Hauryliuk
  SCIENTIFIC REPORTS, Volume：6, First page：35824, Oct. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep35824
  DOI ID：10.1038/srep35824, ISSN：2045-2322, PubMed ID：27775002, Web of Science ID：WOS:000385923600001
• Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis　　　　　　　　　　　　　　　
  Satoshi Yamashita; Haruhiko Yamaguchi; Toshiyuki Waki; Yuichi Aoki; Makie Mizuno; Fumihiro Yanbe; Tomoki Ishii; Ayuta Funaki; Yuzuru Tozawa; Yukino Miyagi-Inoue; Kazuhisa Fushihara; Toru Nakayama; Seiji Takahashi
  ELIFE, Volume：5, Oct. 2016, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.7554/eLife.19022
  DOI ID：10.7554/eLife.19022, ISSN：2050-084X, PubMed ID：27790974, Web of Science ID：WOS:000388660900001
• Atomic force microscope observation of voltage-dependence K+ channel KAT1 reconstructed to supported lipid bilayer　　　　　　　　　　　　　　　
  Suzuki Yuya; Nozawa Akira; Tozawa Yuzuru; Tero Ryugo
  Abstract of annual meeting of the Surface Science of Japan, Volume：36, First page：149, Last page：149, 2016
  支持脂質二重膜(SLB)は細胞膜モデル系として膜タンパク質の挙動や構造を直接観察するために有用である。植物由来の電位依存性K⁺チャネルKAT1の構造と特性を明らかにすることを目的とし、大豆由来の粗精製リン脂質であるasolectinのSLBを作製しKAT1を再構成した。AFM観察によりKAT1に由来する突起物を観察した。SLB作製条件からKAT1の分子配向について議論する。
  The Surface Science Society of Japan, Japanese
  DOI：https://doi.org/10.14886/sssj2008.36.0_149
  DOI ID：10.14886/sssj2008.36.0_149, CiNii Articles ID：130005175554
• Evidence that the Entamoeba histolytica Mitochondrial Carrier Family Links Mitosomal and Cytosolic Pathways through Exchange of 3 '-Phosphoadenosine 5 '-Phosphosulfate and ATP　　　　　　　　　　　　　　　
  Fumika Mi-ichi; Akira Nozawa; Hiroki Yoshida; Yuzuru Tozawa; Tomoyoshi Nozaki
  EUKARYOTIC CELL, Volume：14, Number：11, First page：1144, Last page：1150, Nov. 2015, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1128/EC.00130-15
  DOI ID：10.1128/EC.00130-15, ISSN：1535-9778, eISSN：1535-9786, PubMed ID：26385892, Web of Science ID：WOS:000363814000011
• An enzymatic method to estimate the content of L-hydroxyproline　　　　　　　　　　　　　　　
  Seiya Watanabe; Yoshinobu Hiraoka; Shiori Endo; Yoshiaki Tanimoto; Yuzuru Tozawa; Yasuo Watanabe
  JOURNAL OF BIOTECHNOLOGY, Volume：199, First page：9, Last page：16, Apr. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jbiotec.2015.01.026
  DOI ID：10.1016/j.jbiotec.2015.01.026, ISSN：0168-1656, eISSN：1873-4863, PubMed ID：25678137, Web of Science ID：WOS:000351660600002
• A Novel Mitosomal beta-Barrel Outer Membrane Protein in Entamoeba　　　　　　　　　　　　　　　
  Herbert J. Santos; Kenichiro Imai; Takashi Makiuchi; Kentaro Tomii; Paul Horton; Akira Nozawa; Mohamed Ibrahim; Yuzuru Tozawa; Tomoyoshi Nozaki
  SCIENTIFIC REPORTS, Volume：5, First page：8545, Feb. 2015, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1038/srep08545
  DOI ID：10.1038/srep08545, ISSN：2045-2322, PubMed ID：25711150, Web of Science ID：WOS:000349894400005
• Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase　　　　　　　　　　　　　　　
  Akira Nozawa; Yuzuru Tozawa
  JOURNAL OF BIOSCIENCE AND BIOENGINEERING, Volume：118, Number：2, First page：130, Last page：133, Aug. 2014, [Reviewed], [Last]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.jbiosc.2014.02.001
  DOI ID：10.1016/j.jbiosc.2014.02.001, ISSN：1389-1723, eISSN：1347-4421, PubMed ID：24656877, Web of Science ID：WOS:000341559000003
• Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice　　　　　　　　　　　　　　　
  Yuhta Nomura; Akira Nozawa; Yuzuru Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：78, Number：6, First page：1022, Last page：1025, Jun. 2014, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1080/09168451.2014.910103
  DOI ID：10.1080/09168451.2014.910103, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：25036129, Web of Science ID：WOS:000340133800016
• Diversity in Guanosine 3 ',5 '-Bisdiphosphate (ppGpp) Sensitivity among Guanylate Kinases of Bacteria and Plants　　　　　　　　　　　　　　　
  Yuhta Nomura; Atsushi Izumi; Yoshinori Fukunaga; Kensuke Kusumi; Koh Iba; Seiya Watanabe; Yoichi Nakahira; Andreas P. M. Weber; Akira Nozawa; Yuzuru Tozawa
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：289, Number：22, First page：15631, Last page：15641, May 2014, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.M113.534768
  DOI ID：10.1074/jbc.M113.534768, ISSN：0021-9258, eISSN：1083-351X, PubMed ID：24722991, Web of Science ID：WOS:000337465400045
• Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins　　　　　　　　　　　　　　　
  Akira Nozawa; Yuzuru Tozawa
  Methods in Molecular Biology, Volume：1072, First page：259, Last page：272, 2014, [Reviewed], [Corresponding]
  Humana Press Inc., English, Scientific journal
  DOI：https://doi.org/10.1007/978-1-62703-631-3_19
  DOI ID：10.1007/978-1-62703-631-3_19, ISSN：1064-3745, PubMed ID：24136528, SCOPUS ID：84934434965
• Identification and characterization of trans-3-hydroxy-L-proline dehydratase and Delta(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-L-proline metabolism of bacteria　　　　　　　　　　　　　　　
  Seiya Watanabe; Yoshiaki Tanimoto; Seiji Yamauchi; Yuzuru Tozawa; Shigeki Sawayama; Yasuo Watanabe
  FEBS OPEN BIO, Volume：4, First page：240, Last page：250, 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.fob.2014.02.010
  DOI ID：10.1016/j.fob.2014.02.010, ISSN：2211-5463, PubMed ID：24649405, Web of Science ID：WOS:000346278200030
• Ornithine cyclodeaminase/mu-crystallin homolog from the hyperthermophilic archaeon Thermococcus litoralis functions as a novel Delta(1)-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-L-proline metabolism　　　　　　　　　　　　　　　
  Seiya Watanabe; Yuzuru Tozawa; Yasuo Watanabe
  FEBS OPEN BIO, Volume：4, First page：617, Last page：626, 2014, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.fob.2014.07.005
  DOI ID：10.1016/j.fob.2014.07.005, ISSN：2211-5463, PubMed ID：25161870, Web of Science ID：WOS:000346278200073
• Theophylline-dependent riboswitch as a novel genetic tool for strict regulation of protein expression in cyanobacterium synechococcus elongatus PCC 7942　　　　　　　　　　　　　　　
  Yoichi Nakahira; Atsushi Ogawa; Hiroyuki Asano; Tokitaka Oyama; Yuzuru Tozawa
  Plant and Cell Physiology, Volume：54, Number：10, First page：1724, Last page：1735, Oct. 2013, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pct115
  DOI ID：10.1093/pcp/pct115, ISSN：0032-0781, PubMed ID：23969558, SCOPUS ID：84885929738
• Overproduction of Hyperthermostable beta-1,4-Endoglucanase from the Archaeon Pyrococcus horikoshii by Tobacco Chloroplast Engineering　　　　　　　　　　　　　　　
  Yoichi Nakahira; Kazuhiko Ishikawa; Kunisuke Tanaka; Yuzuru Tozawa; Takashi Shiina
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：77, Number：10, First page：2140, Last page：2143, Oct. 2013, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.130413
  DOI ID：10.1271/bbb.130413, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：24096651, Web of Science ID：WOS:000326486500028
• Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum　　　　　　　　　　　　　　　
  Shin Kore-eda; Akira Nozawa; Yusuke Okada; Kazuki Takashi; Muhammad Abul Kalam Azad; Jun-ichi Ohnishi; Yoshitaka Nishiyama; Yuzuru Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：77, Number：7, First page：1511, Last page：1516, Jul. 2013, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.130174
  DOI ID：10.1271/bbb.130174, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：23832369, Web of Science ID：WOS:000323533800026
• Cell-free expression - making a mark　　　　　　　　　　　　　　　
  Frank Bernhard; Yuzuru Tozawa
  CURRENT OPINION IN STRUCTURAL BIOLOGY, Volume：23, Number：3, First page：374, Last page：380, Jun. 2013, [Reviewed], [Last]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.sbi.2013.03.012
  DOI ID：10.1016/j.sbi.2013.03.012, ISSN：0959-440X, PubMed ID：23628286, Web of Science ID：WOS:000321408300008
• Effect of counter ions on the transport current across membranes containing KAT1 potassium channel　　　　　　　　　　　　　　　
  Shintaro Kubota; Osamu Shirai; Takao Hibi; Yuzuru Tozawa; Kenji Kano
  Analytical Sciences, Volume：29, Number：1, First page：161, Last page：164, 2013, [Reviewed]
  English
  DOI：https://doi.org/10.2116/analsci.29.161
  DOI ID：10.2116/analsci.29.161, ISSN：0910-6340, PubMed ID：23303104, SCOPUS ID：84873869267
• Identification and Characterization of D-Hydroxyproline Dehydrogenase and Delta(1)-Pyrroline-4-hydroxy-2-carboxylate Deaminase Involved in Novel L-Hydroxyproline Metabolism of Bacteria METABOLIC CONVERGENT EVOLUTION　　　　　　　　　　　　　　　
  Seiya Watanabe; Daichi Morimoto; Fumiyasu Fukumori; Hiroto Shinomiya; Hisashi Nishiwaki; Miyuki Kawano-Kawada; Yuuki Sasai; Yuzuru Tozawa; Yasuo Watanabe
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：287, Number：39, First page：32674, Last page：32688, Sep. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1074/jbc.M112.374272
  DOI ID：10.1074/jbc.M112.374272, ISSN：0021-9258, PubMed ID：22833679, Web of Science ID：WOS:000309168300035
• Exploration of a Possible Partnership among Orphan Two-Component System Proteins in Cyanobacterium Synechococcus elongatus PCC 7942　　　　　　　　　　　　　　　
  Hiroaki Kato; Satoru Watanabe; Kaori Nimura-Matsune; Taku Chibazakura; Yuzuru Tozawa; Hirofumi Yoshikawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：76, Number：8, First page：1484, Last page：1491, Aug. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.120172
  DOI ID：10.1271/bbb.120172, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：22878191, Web of Science ID：WOS:000308835000010
• Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp(0) mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome　　　　　　　　　　　　　　　
  Kazumi Tagami; Hideaki Nanamiya; Yuka Kazo; Marie Maehashi; Shota Suzuki; Eri Namba; Masahiro Hoshiya; Ryo Hanai; Yuzuru Tozawa; Takuya Morimoto; Naotake Ogasawara; Yasushi Kageyama; Katsutoshi Ara; Katsuya Ozaki; Masaki Yoshida; Haruko Kuroiwa; Tsuneyoshi Kuroiwa; Yoshiaki Ohashi; Fujio Kawamura
  MICROBIOLOGYOPEN, Volume：1, Number：2, First page：115, Last page：134, Jun. 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1002/mbo3.16
  DOI ID：10.1002/mbo3.16, ISSN：2045-8827, PubMed ID：22950019, Web of Science ID：WOS:000209077500004
• Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase　　　　　　　　　　　　　　　
  Yusuke Yagi; Yoko Ishizaki; Yoichi Nakahira; Yuzuru Tozawa; Takashi Shiina
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Volume：109, Number：19, First page：7541, Last page：7546, May 2012, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1073/pnas.1119403109
  DOI ID：10.1073/pnas.1119403109, ISSN：0027-8424, PubMed ID：22529394, Web of Science ID：WOS:000304090600082
• ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro　　　　　　　　　　　　　　　
  Yuhta Nomura; Taito Takabayashi; Hiroshi Kuroda; Yasushi Yukawa; Kwanchanok Sattasuk; Mitsuru Akita; Akira Nozawa; Yuzuru Tozawa
  PLANT MOLECULAR BIOLOGY, Volume：78, Number：1-2, First page：185, Last page：196, Jan. 2012, [Reviewed], [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s11103-011-9858-x
  DOI ID：10.1007/s11103-011-9858-x, ISSN：0167-4412, PubMed ID：22108865, Web of Science ID：WOS:000297815400014
• In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization　　　　　　　　　　　　　　　
  Kwanchanok Sattasuk; Akira Nozawa; Yuzuru Tozawa; Yoshimi Kakinuma; Mitsuru Akita
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：75, Number：11, First page：2200, Last page：2206, Nov. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.110489
  DOI ID：10.1271/bbb.110489, ISSN：0916-8451, eISSN：1347-6947, PubMed ID：22056429, Web of Science ID：WOS:000297610500020
• Cell-free synthesis, reconstitution, and characterization of a mitochondrial dicarboxylate-tricarboxylate carrier of Plasmodium falciparum　　　　　　　　　　　　　　　
  Akira Nozawa; Ryoji Fujimoto; Hiroki Matsuoka; Takafumi Tsuboi; Yuzuru Tozawa
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume：414, Number：3, First page：612, Last page：617, Oct. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.bbrc.2011.09.130
  DOI ID：10.1016/j.bbrc.2011.09.130, ISSN：0006-291X, PubMed ID：21986531, Web of Science ID：WOS:000298519500029
• Signalling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants　　　　　　　　　　　　　　　
  Y. Tozawa; Y. Nomura
  Plant Biology, Volume：13, Number：5, First page：699, Last page：709, Sep. 2011, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1438-8677.2011.00484.x
  DOI ID：10.1111/j.1438-8677.2011.00484.x, ISSN：1435-8603, PubMed ID：21815973, SCOPUS ID：79961029191
• Differences of two polychaete species reflected in enzyme activities　　　　　　　　　　　　　　　
  Katsutoshi Ito; Mana Nozaki; Takashi Ohta; Chiemi Miura; Yuzuru Tozawa; Takeshi Miura
  MARINE BIOLOGY, Volume：158, Number：6, First page：1211, Last page：1221, Jun. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00227-011-1641-7
  DOI ID：10.1007/s00227-011-1641-7, ISSN：0025-3162, eISSN：1432-1793, Web of Science ID：WOS:000290806500003
• Co-translational function of Cosmc, core 1 synthase specific molecular chaperone, revealed by a cell-free translation system　　　　　　　　　　　　　　　
  Yoshiki Narimatsu; Tomomi Kubota; Sanae Furukawa; Mie Shimojima; Hiroko Iwasaki; Yuzuru Tozawa; Kouichi Tachibana; Hisashi Narimatsu
  FEBS LETTERS, Volume：585, Number：9, First page：1276, Last page：1280, May 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.febslet.2011.04.010
  DOI ID：10.1016/j.febslet.2011.04.010, ISSN：0014-5793, CiNii Articles ID：80021796477, PubMed ID：21496458, Web of Science ID：WOS:000290248300004
• Anti-viral effects of interferon administration on sevenband grouper, Epinephelus septemfasciatus　　　　　　　　　　　　　　　
  Takashi Ohta; Yusuke Ueda; Katsutoshi Ito; Chiemi Miura; Hirofumi Yamashita; Takeshi Miura; Yuzuru Tozawa
  FISH & SHELLFISH IMMUNOLOGY, Volume：30, Number：4-5, First page：1064, Last page：1071, Apr. 2011, [Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.fsi.2011.02.003
  DOI ID：10.1016/j.fsi.2011.02.003, ISSN：1050-4648, PubMed ID：21316457, Web of Science ID：WOS:000289969000009
• Tolerance of Spermatogonia to Oxidative Stress Is Due to High Levels of Zn and Cu/Zn Superoxide Dismutase　　　　　　　　　　　　　　　
  Fritzie T. Celino; Sonoko Yamaguchi; Chiemi Miura; Takashi Ohta; Yuzuru Tozawa; Toshiharu Iwai; Takeshi Miura
  PLOS ONE, Volume：6, Number：2, First page：e16938, Feb. 2011, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1371/journal.pone.0016938
  DOI ID：10.1371/journal.pone.0016938, ISSN：1932-6203, Web of Science ID：WOS:000287482800022
• Ribosome rescue by Escherichia coli ArfA (YhdL) in the absence of trans-translation system　　　　　　　　　　　　　　　
  Yuhei Chadani; Katsuhiko Ono; Shin-ichiro Ozawa; Yuichiro Takahashi; Kazuyuki Takai; Hideaki Nanamiya; Yuzuru Tozawa; Kazuhiro Kutsukake; Tatsuhiko Abo
  MOLECULAR MICROBIOLOGY, Volume：78, Number：4, First page：796, Last page：808, Nov. 2010, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-2958.2010.07375.x
  DOI ID：10.1111/j.1365-2958.2010.07375.x, ISSN：0950-382X, CiNii Articles ID：80021439724, Web of Science ID：WOS:000284072200003
• Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents　　　　　　　　　　　　　　　
  Takahisa Genji; Akira Nozawa; Yuzuru Tozawa
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume：400, Number：4, First page：638, Last page：642, Oct. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.bbrc.2010.08.119
  DOI ID：10.1016/j.bbrc.2010.08.119, ISSN：0006-291X, CiNii Articles ID：80021313088, PubMed ID：20807510, Web of Science ID：WOS:000282850500032
• Control of translational initiation in the wheat-embryo cell-free protein expression system for producing homogenous products　　　　　　　　　　　　　　　
  Takashi Ohta; Hiroki Matsuoka; Yuhta Nomura; Yuzuru Tozawa
  PROTEIN EXPRESSION AND PURIFICATION, Volume：73, Number：1, First page：15, Last page：22, Sep. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.pep.2010.03.011
  DOI ID：10.1016/j.pep.2010.03.011, ISSN：1046-5928, CiNii Articles ID：10030014301, PubMed ID：20304073, Web of Science ID：WOS:000279312700003
• The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system　　　　　　　　　　　　　　　
  Seiji Yamauchi; Naoki Fusada; Hidenori Hayashi; Toshihiko Utsumi; Nobuyuki Uozumi; Yaeta Endo; Yuzuru Tozawa
  FEBS JOURNAL, Volume：277, Number：17, First page：3596, Last page：3607, Sep. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1742-4658.2010.07768.x
  DOI ID：10.1111/j.1742-4658.2010.07768.x, ISSN：1742-464X, CiNii Articles ID：80021279820, PubMed ID：20716180, Web of Science ID：WOS:000280996300014
• Cell-free protein N-myristoylation system utilizing wheat-germ extracts facilitates update of consensus motif for the modification of plant proteins　　　　　　　　　　　　　　　
  Yamauchi, S; Fusada, N; Hayashi, H; Utsumi, T; Uozumi, N; Tozawa, Y
  FEBS. J., Jul. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
• Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis　　　　　　　　　　　　　　　
  Yasutaka Nishiyama; Choong-Soo Yun; Fumio Matsuda; Tadamasa Sasaki; Kazuki Saito; Yuzuru Tozawa
  PLANTA, Volume：232, Number：1, First page：209, Last page：218, Jun. 2010, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1007/s00425-010-1166-1
  DOI ID：10.1007/s00425-010-1166-1, ISSN：0032-0935, CiNii Articles ID：80021069995, Web of Science ID：WOS:000277792700018
• Transcription Activity of Individual rrn Operons in Bacillus subtilis Mutants Deficient in (p)ppGpp Synthetase Genes, relA, yjbM, and ywaC　　　　　　　　　　　　　　　
  Yousuke Natori; Kazumi Tagami; Kana Murakami; Sawako Yoshida; Osamu Tanigawa; Yoonsuh Moh; Kenta Masuda; Tetsuya Wada; Shota Suzuki; Hideaki Nanamiya; Yuzuru Tozawa; Fujio Kawamura
  JOURNAL OF BACTERIOLOGY, Volume：191, Number：14, First page：4555, Last page：4561, Jul. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1128/JB.00263-09
  DOI ID：10.1128/JB.00263-09, ISSN：0021-9193, CiNii Articles ID：80020423770, PubMed ID：19447912, Web of Science ID：WOS:000267372900008
• Novel Bacterial N-Acetyltransferase Gene for Herbicide Detoxification in Land Plants and Selection Maker in Plant Transformation　　　　　　　　　　　　　　　
  Choong-Soo Yun; Hisakazu Hasegawa; Hideaki Nanamiya; Teruhiko Terakawa; Yuzuru Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：73, Number：5, First page：1000, Last page：1006, May 2009, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.80777
  DOI ID：10.1271/bbb.80777, ISSN：0916-8451, eISSN：1347-6947, CiNii Articles ID：10027540472, Web of Science ID：WOS:000266620100005
• The contribution of endogenous cellulase to the cellulose digestion in the gut of earthworm (Pheretima hilgendorfi: Megascolecidae)　　　　　　　　　　　　　　　
  Mana Nozaki; Chiemi Miura; Yuzuru Tozawa; Takeshi Miura
  SOIL BIOLOGY & BIOCHEMISTRY, Volume：41, Number：4, First page：762, Last page：769, Apr. 2009, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1016/j.soilbio.2009.01.016
  DOI ID：10.1016/j.soilbio.2009.01.016, ISSN：0038-0717, CiNii Articles ID：80020272776, Web of Science ID：WOS:000265325300013
• (3) The Role of the Earthworm, Pheretima (Metaphire) hilgendorfi, in Terrestrial Ecosystem Nutrient Cycling.　　　　　　　　　　　　　　　
  Nozaki Mana; Miura Chiemi; Tozawa Yuzuru; Miura Takeshi
  Interdisciplinary Studies on Environmental Chemistry— Biological Responses to Chemical Pollutants, First page：275, Last page：279, Dec. 2008, [Reviewed]
  English, International conference proceedings
• Mutation of a rice gene encoding a phenylalanine biosynthetic enzyme results in accumulation of phenylalanine and tryptophan　　　　　　　　　　　　　　　
  Tetsuya Yamada; Fumio Matsuda; Koji Kasai; Shuichi Fukuoka; Keisuke Kitamura; Yuzuru Tozawa; Hisashi Miyagawa; Kyo Wakasa
  PLANT CELL, Volume：20, Number：5, First page：1316, Last page：1329, May 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1105/tpc.107.057455
  DOI ID：10.1105/tpc.107.057455, ISSN：1040-4651, eISSN：1532-298X, Web of Science ID：WOS:000257320600012
• Expression of parsley flavone synthase I establishes the flavone Biosynthetic pathway in Arabidopsis thaliana　　　　　　　　　　　　　　　
  Choong-Soo Yun; Tomio Yamamoto; Akira Nozawa; Yuzuru Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：72, Number：4, First page：968, Last page：973, Apr. 2008, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1271/bbb.70692
  DOI ID：10.1271/bbb.70692, ISSN：0916-8451, eISSN：1347-6947, CiNii Articles ID：10027526457, Web of Science ID：WOS:000255501100007
• The bacterial stringent response, conserved in chloroplasts, controls plant fertilization　　　　　　　　　　　　　　　
  Shinji Masuda; Kazuki Mizusawa; Takakuni Narisawa; Yuzuru Tozawa; Hiroyuki Ohta; Ken-ichiro Takamiya
  PLANT AND CELL PHYSIOLOGY, Volume：49, Number：2, First page：135, Last page：141, Feb. 2008, [Reviewed]
  English, Scientific journal
  DOI：https://doi.org/10.1093/pcp/pcm177
  DOI ID：10.1093/pcp/pcm177, ISSN：0032-0781, CiNii Articles ID：10027328528, PubMed ID：18178586, Web of Science ID：WOS:000253021400001
• Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis　　　　　　　　　　　　　　　
  Hideaki Nanamiya; Koji Kasai; Akira Nozawa; Choong-Soo Yun; Takakuni Narisawa; Kana Murakami; Yousuke Natori; Fujio Kawamura; Yuzuru Tozawa
  MOLECULAR MICROBIOLOGY, Volume：67, Number：2, First page：291, Last page：304, Jan. 2008, [Reviewed], [Last, Corresponding]
  English, Scientific journal
  DOI：https://doi.org/10.1111/j.1365-2958.2007.06018.x
  DOI ID：10.1111/j.1365-2958.2007.06018.x, ISSN：0950-382X, Web of Science ID：WOS:000251765900008
• Possible targets of "magic spots" in plant signalling　　　　　　　　　　　　　　　
  Shinji Masuda; Yuzuru Tozawa; Hiroyuki Ohta
  Plant Signaling and Behavior, Volume：3, Number：11, First page：1021, Last page：1023, 2008, [Reviewed]
  Landes Bioscience, English, Scientific journal
  DOI：https://doi.org/10.4161/psb.6766
  DOI ID：10.4161/psb.6766, ISSN：1559-2324, PubMed ID：19704441, SCOPUS ID：56049119193
• Calcium-activated (p)ppGpp synthetase in chloroplasts of land plants　　　　　　　　　　　　　　　
  Yuzuru Tozawa; Akira Nozawa; Takuya Kanno; Takakuni Narisawa; Shinji Masuda; Koji Kasai; Hideaki Nanamiya
  JOURNAL OF BIOLOGICAL CHEMISTRY, Volume：282, Number：49, First page：35536, Last page：35545, Dec. 2007, [Reviewed], [Lead, Corresponding]
  English, Scientific journal
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  DOI ID：10.1074/jbc.M703820200, ISSN：0021-9258, PubMed ID：17938177, Web of Science ID：WOS:000251458100013
• A cell-free translation and proteoliposome reconstitution system for functional analysis of plant solute transporters　　　　　　　　　　　　　　　
  Akira Nozawa; Hideaki Nanamiya; Takuji Miyata; Nicole Linka; Yaeta Endo; Andreas P. M. Weber; Yuzuru Tozawa
  PLANT AND CELL PHYSIOLOGY, Volume：48, Number：12, First page：1815, Last page：1820, Dec. 2007, [Reviewed], [Corresponding]
  English, Scientific journal
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• The plastid sigma factor SIG1 maintains photosystem I activity via regulated expression of the psaA operon in rice chloroplasts　　　　　　　　　　　　　　　
  Yuzuru Tozawa; Masayoshi Teraishi; Tadamasa Sasaki; Kintake Sonoike; Yoshitaka Nishiyama; Mitsuhiro Itaya; Akio Miyao; Hirohiko Hirochika
  PLANT JOURNAL, Volume：52, Number：1, First page：124, Last page：132, Oct. 2007, [Lead, Corresponding]
  English, Scientific journal
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• Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II　　　　　　　　　　　　　　　
  Kouji Kojima; Masaru Oshita; Yohei Nanjo; Koji Kasai; Yuzuru Tozawa; Hidenori Hayashi; Yoshitaka Nishiyama
  MOLECULAR MICROBIOLOGY, Volume：65, Number：4, First page：936, Last page：947, Aug. 2007, [Reviewed]
  English, Scientific journal
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• Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system　　　　　　　　　　　　　　　
  Takuya Kanno; Michiko Kitano; Rika Kato; Akira Omori; Yaeta Endo; Yuzuru Tozawa
  PROTEIN EXPRESSION AND PURIFICATION, Volume：52, Number：1, First page：59, Last page：65, Mar. 2007, [Reviewed], [Last, Corresponding]
  English, Scientific journal
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• Functional similarities of a thermostable protein-disulfide oxidoreductase identified in the archaeon Pyrococcus horikoshii to bacterial DsbA enzymes　　　　　　　　　　　　　　　
  Toshihiro Kuroita; Takuya Kanno; Atsushi Kawai; Bunsei Kawakami; Masanori Oka; Yaeta Endo; Yuzuru Tozawa
  EXTREMOPHILES, Volume：11, Number：1, First page：85, Last page：94, Jan. 2007, [Reviewed], [Last, Corresponding]
  English, Scientific journal
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• Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice: Tissue-specific and light-responsive gene expression　　　　　　　　　　　　　　　
  Yoshiki Kubota; Akio Miyao; Hirohiko Hirochika; Yuzuru Tozawa; Hiroyuki Yasuda; Yuichi Tsunoyama; Yasuo Niwa; Sousuke Imamura; Makoto Shirai; Munehiko Asayama
  PLANT AND CELL PHYSIOLOGY, Volume：48, Number：1, First page：186, Last page：192, Jan. 2007, [Reviewed]
  English, Scientific journal
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• Roles of 11 beta-hydroxysteroid dehydrogenase in fish spermatogenesis　　　　　　　　　　　　　　　
  Yuichi Ozaki; Masato Higuchi; Chiemi Miura; Sonoko Yamaguchi; Yuzuru Tozawa; Takeshi Miura
  ENDOCRINOLOGY, Volume：147, Number：11, First page：5139, Last page：5146, Nov. 2006, [Reviewed]
  English, Scientific journal
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• Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract　　　　　　　　　　　　　　　
  Shin-ichi Makino; Tatsuya Sawasaki; Yuzuru Tozawa; Yaeta Endo; Kazuyuki Takai
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Volume：347, Number：4, First page：1080, Last page：1087, Sep. 2006, [Reviewed]
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• High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile　　　　　　　　　　　　　　　
  Kyo Wakasa; Hisakazu Hasegawa; Hiroshi Nemoto; Fumio Matsuda; Haruna Miyazawa; Yuzuru Tozawa; Keiko Morino; Akira Komatsu; Tetsuya Yamada; Teruhiko Terakawa; Hisashi Miyagawa
  JOURNAL OF EXPERIMENTAL BOTANY, Volume：57, Number：12, First page：3069, Last page：3078, Sep. 2006, [Reviewed]
  English, Scientific journal
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• Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: Limited interdomain misfolding in a eukaryotic translation system　　　　　　　　　　　　　　　
  N Hirano; T Sawasaki; Y Tozawa; Y Endo; K Takai
  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Volume：64, Number：2, First page：343, Last page：354, Aug. 2006, [Reviewed]
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• Identification of three shikimate kinase genes in rice: characterization of their differential expression during panicle development and of the enzymatic activities of the encoded proteins　　　　　　　　　　　　　　　
  K Kasai; T Kanno; M Akita; Y Ikejiri-Kanno; K Wakasa; Y Tozawa
  PLANTA, Volume：222, Number：3, First page：438, Last page：447, Oct. 2005, [Reviewed], [Last, Corresponding]
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• Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway　　　　　　　　　　　　　　　
  T Kanno; A Komatsu; K Kasai; JG Dubouzet; M Sakurai; Y Ikejiri-Kanno; K Wakasa; Y Tozawa
  PLANT PHYSIOLOGY, Volume：138, Number：4, First page：2260, Last page：2268, Aug. 2005, [Reviewed], [Last, Corresponding]
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• Use of a feedback-insensitive at subunit of anthranilate synthase as a selectable marker for transformation of rice and potato　　　　　　　　　　　　　　　
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  MOLECULAR BREEDING, Volume：14, Number：4, First page：363, Last page：373, Nov. 2004, [Reviewed]
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• Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp　　　　　　　　　　　　　　　
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  PLANT AND CELL PHYSIOLOGY, Volume：45, Number：9, First page：1194, Last page：1201, Sep. 2004, [Reviewed]
  English, Scientific journal
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• The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation　　　　　　　　　　　　　　　
  H Sugimoto; K Kusumi; Y Tozawa; J Yazaki; N Kishimoto; S Kikuchi; K Iba
  PLANT AND CELL PHYSIOLOGY, Volume：45, Number：8, First page：985, Last page：996, Aug. 2004, [Reviewed]
  English, Scientific journal
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• Proteomics of the rice cell: systematic identification of the protein populations in subcellular compartments　　　　　　　　　　　　　　　
  N Tanaka; M Fujita; H Handa; S Murayama; M Uemura; Y Kawamura; T Mitsui; S Mikami; Y Tozawa; T Yoshinaga; S Komatsu
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• Differential expression of three plastidial signia factors, OsSIG1, OsSIG2A, and OsSIG2B, during leaf development in rice　　　　　　　　　　　　　　　
  K Kasai; M Kawagishi-Kobayashi; M Teraishi; Y Ito; K Ochi; K Wakasa; Y Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：68, Number：4, First page：973, Last page：977, Apr. 2004, [Reviewed], [Last, Corresponding]
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• Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline　　　　　　　　　　　　　　　
  K Kasai; T Kanno; Y Endo; K Wakasa; Y Tozawa
  NUCLEIC ACIDS RESEARCH, Volume：32, Number：19, First page：5732, Last page：5741, 2004, [Reviewed], [Last, Corresponding]
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• In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the alpha subunits OASA1 and OASA2　　　　　　　　　　　　　　　
  T Kanno; K Kasai; Y Ikejiri-Kanno; K Wakasa; Y Tozawa
  PLANT MOLECULAR BIOLOGY, Volume：54, Number：1, First page：11, Last page：23, Jan. 2004, [Reviewed], [Last, Corresponding]
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• Ribosome engineering and secondary metabolite production　　　　　　　　　　　　　　　
  K Ochi; S Okamoto; Y Tozawa; T Inaoka; T Hosaka; J Xu; K Kurosawa
  ADVANCES IN APPLIED MICROBIOLOGY, VOL 56, Volume：56, First page：155, Last page：+, 2004
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• Expression profiling of translation-associated genes in sporulating Bacillus subtilis and consequence of sporulation by gene inactivation　　　　　　　　　　　　　　　
  Y Ohashi; T Inaoka; K Kasai; Y Ito; S Okamoto; H Satsu; Y Tozawa; F Kawamura; K Ochi
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：67, Number：10, First page：2245, Last page：2253, Oct. 2003, [Reviewed]
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• OsRALyase1, a putative F-box protein identified in rice, Oryza sativa, with enzyme activity identical to that of wheat RALyase　　　　　　　　　　　　　　　
  Y Ito; A Ozawa; T Sawasaki; Y Endo; K Ochi; Y Tozawa
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Volume：66, Number：12, First page：2727, Last page：2731, Dec. 2002, [Reviewed], [Corresponding]
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• A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro　　　　　　　　　　　　　　　
  K Kasai; S Usami; T Yamada; Y Endo; K Ochi; Y Tozawa
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• A rifampicin resistance mutation in the rpoB gene confers ppGpp-independent antibiotic production in Streptomyces coelicolor A3(2)　　　　　　　　　　　　　　　
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• Genetic and physiological characterization of rpoB mutations that activate antibiotic production in Streptomyces lividans　　　　　　　　　　　　　　　
  Caixia Lai; Jun Xu; Yuzuru Tozawa; Yoshiko Okamoto-Hosoya; Xingsheng Yao; Kozo Ochi
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• Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing mutant of OASA1　　　　　　　　　　　　　　　
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• Efficient transformation of suspension-cultured rice cells mediated by Agrobacterium tumefaciens
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• The calcium-independent protein kinase C participates in an early process of CD3/CD28-mediated induction of thymocyte apoptosis　　　　　　　　　　　　　　　
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• Nuclear encoding of a plastid sigma factor in rice and its tissue- and light-dependent expression　　　　　　　　　　　　　　　
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• Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arabidopsis thaliana: Evidence for the sigma factor heterogeneity in higher plant plastids　　　　　　　　　　　　　　　
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• Differential induction of helper and killer T cells from isolated CD4(+)CD8(+) thymocytes in suspension culture　　　　　　　　　　　　　　　
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• In vitro differentiation and commitment of CD4+CD8+ thymocytes to the CD4 lineage without TCR engagement　　　　　　　　　　　　　　　
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• DISTINCT ROLES OF THE RECEPTOR TYROSINE KINASES TIE-1 AND TIE-2 IN BLOOD-VESSEL FORMATION　　　　　　　　　　　　　　　
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• CALCINEURIN ACTIVATION PROTECTS T-CELLS FROM GLUCOCORTICOID-INDUCED APOPTOSIS　　　　　　　　　　　　　　　
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• INVOLVEMENT OF PROTEIN KINASE-C-EPSILON IN GLUCOCORTICOID-INDUCED APOPTOSIS IN THYMOCYTES　　　　　　　　　　　　　　　
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• Isolation and Characterization of the gro ES and groEL genes of Bacillus subtilis Marburg　　　　　　　　　　　　　　　
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• Model-Free Idealization: Adaptive Integrated Approach for Idealization of Ion Channel Currents (AI2)　　　　　　　　　　　　　　　
  Madoka Sato; Masanori Hariyama; Komiya Maki; Kae Suzuki; Yuzuru Tozawa; Hideaki Yamamoto; Ayumi Hirano-Iwata
  arXiv, 14 Feb. 2023
  English
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• Solubilization of natural rubber biosynthetic enzyme complexes from rubber particles of the Para rubber tree (Hevea brasiliensis) by the amphiphilic copolymer treatments　　　　　　　　　　　　　　　
  KHAN Nadia Nur Shazana Binti Abu Talib; 小島幸治; 山口晴彦; 三上智世; 廣森美樹; 和氣駿之; 宮城ゆき乃; 山下哲; 戸澤譲; 中山亨; 高橋征司
  日本植物生理学会年会(Web), Volume：64th, 2023
  J-Global ID：202302261107495062
• 高等植物のシス型プレニルトランスフェラーゼの活性制御におけるcPT-like proteinの役割　　　　　　　　　　　　　　　
  KHAN Nadia Nur Shazana Binti Abu Talib; 高橋朋宏; 廣森美樹; 山口真琴; 皆川知歩; 山家史大; 大場崇史; 山下哲; 戸澤譲; 山口晴彦; 宮城ゆき乃; 和氣駿之; 中山亨; 高橋征司
  Volume：32nd (CD-ROM), 2022
  J-Global ID：202202210485248037
• 脂肪滴およびゴム粒子の構造維持に関わる膜タンパク質の比較解析　　　　　　　　　　　　　　　
  中山隆司; 開琢海; 三輪幸祐; 廣森美樹; 和氣駿之; 山下哲; 戸澤譲; 山口晴彦; 宮城ゆき乃; 岩井雅子; 太田啓之; 中山亨; 高橋征司
  Volume：95th, 2022
  J-Global ID：202302287459103588
• Identification and functional analysis of interaction domains of rubber transferase protein complex from Hevea brasiliensis　　　　　　　　　　　　　　　
  NUR SHAZANA BINTI ABU TALIB KHAN Nadia; 廣森美樹; 和氣駿之; 山下哲; 戸澤譲; 山口晴彦; 宮城ゆき乃; 中山亨; 高橋征司
  日本農芸化学会大会講演要旨集(Web), Volume：2022, 2022
  ISSN：2186-7976, J-Global ID：202202225311742510
• Functional analysis for reconstituted rubber synthetic enzyme on nanodiscs　　　　　　　　　　　　　　　
  黒岩風; 西野輝; 廣森美樹; MANDAL Yasuko; 山口晴彦; 宮城ゆき乃; 山下哲; 高橋征司; 戸澤譲
  日本農芸化学会大会講演要旨集(Web), Volume：2021, 2021
  ISSN：2186-7976, J-Global ID：202102231726715483
• In vitro biosynthesis of isoprenoid polymers by trans-prenyltransferases identified from Manilkara zapota　　　　　　　　　　　　　　　
  三輪幸祐; 廣森美樹; 青木裕一; 和氣駿之; 小島幸治; 山下哲; 山口晴彦; 宮城ゆき乃; 戸澤譲; 中山亨; 高橋征司
  日本農芸化学会大会講演要旨集(Web), Volume：2021, 2021
  ISSN：2186-7976, J-Global ID：202102247197845981
• Establishment of a system for in vitro translation-coupled external protein introduction into lipid droplets from Chlamydomonas reinhardtii towards elucidation of lipid droplet transit sequence motifs.　　　　　　　　　　　　　　　
  開琢海; 廣森美樹; 和氣駿之; 山下哲; 戸澤譲; 山口晴彦; 宮城ゆき乃; 岩井雅子; 太田啓之; 中山亨; 高橋征司
  日本農芸化学会大会講演要旨集(Web), Volume：2021, 2021
  ISSN：2186-7976, J-Global ID：202102256839519018
• サポジラ(Manilkara zapota)由来trans型プレニルトランスフェラーゼの酵素機能解析　　　　　　　　　　　　　　　
  三輪幸祐; 廣森美樹; 青木裕一; 和氣駿之; 小島幸治; 山下哲; 山口晴彦; 宮城ゆき乃; 戸澤譲; 中山亨; 高橋征司
  Volume：31st (CD-ROM), 2021
  J-Global ID：202102215838782832
• パラゴムノキおよびグアユール由来タンパク質によるナノディスク上へのプレニルトランスフェラーゼ活性の再構成　　　　　　　　　　　　　　　
  黒岩風; 西野輝; 廣森美樹; モンドル慶子; 山口晴彦; 宮城ゆき乃; 山下哲; 高橋征司; 戸澤譲
  Volume：31st (CD-ROM), 2021
  J-Global ID：202102248299172157
• 無細胞翻訳系を用いた脂肪滴への外来酵素導入によるイソプレノイドポリマー生産　　　　　　　　　　　　　　　
  開琢海; 三輪幸祐; 廣森美樹; 和氣駿之; 山下哲; 戸澤譲; 山口晴彦; 宮城ゆき乃; 岩井雅子; 太田啓之; 中山亨; 高橋征司
  Volume：31st (CD-ROM), 2021
  J-Global ID：202102253026207253
• 完全インビトロ再構成系によるパラゴムノキ(Hevea brasiliensis)由来ポリイソプレン合成酵素の機能解析　　　　　　　　　　　　　　　
  黒岩風; 西野輝; 廣森美樹; 山口晴彦; 宮城ゆき乃; 山下哲; 高橋征司; 戸澤譲
  Volume：30th, 2020
  J-Global ID：202002246313093860
• Functional analysis for reconstituted rubber synthesis enzyme　　　　　　　　　　　　　　　
  黒岩風; 西野輝; 廣森美樹; MANDAL Yasuko; 山口晴彦; 宮城ゆき乃; 山下哲; 高橋征司; 戸澤譲
  日本農芸化学会大会講演要旨集(Web), Volume：2020, 2020
  ISSN：2186-7976, J-Global ID：202002249198737453
• Trial for in vitro reconstitution of rubber synthesis machinery　　　　　　　　　　　　　　　
  西野輝; 黒岩風; MANDAL Yasuko; 山口晴彦; 宮城ゆき乃; 山下哲; 高橋誠司; 戸澤譲
  日本農芸化学会大会講演要旨集(Web), Volume：2020, 2020
  ISSN：2186-7976, J-Global ID：202002284391363210
• 長鎖シス型プレニルトランスフェラーゼのパートナーであるNogo-B receptorファミリーのタンパク質構造について　　　　　　　　　　　　　　　
  矢内太朗; 今泉璃城; 高畑佳佑; 山口晴彦; 宮城ゆき乃; 竹下浩平; 戸澤譲; 高橋征司; 山下哲
  Volume：30th, 2020
  J-Global ID：202002235766899575
• Development of a hERG channel sensor for evaluation of drug side effects　　　　　　　　　　　　　　　
  渡辺恭也; 横田澪央; 加藤美生; 佐藤まどか; 但木大介; 小宮麻希; 山本英明; 井上遥香; 戸澤譲; 庭野道夫; 平野愛弓
  電子情報通信学会技術研究報告, Volume：119, Number：431(CPM2019 91-109), 2020
  ISSN：0913-5685, J-Global ID：202002258145057027
• Construction of an artificial cell membrane device and its application to a sensor for drug side effects　　　　　　　　　　　　　　　
  平野 愛弓; 但木 大介; 山浦 大地; 加藤 美生; 小宮 麻希; 井上 遥香; 戸澤 譲; 馬 騰; 山本 英明; 庭野 道夫
  Volume：119, Number：9, First page：23, Last page：26, 18 Apr. 2019
  Japanese
  ISSN：0913-5685, CiNii Articles ID：40021885351, CiNii Books ID：AN10012954
• パラゴムノキの天然ゴム生合成に関与するcis-prenyltransferaseとパートナータンパク質間の相互作用解析　　　　　　　　　　　　　　　
  今泉璃城; 山口晴彦; 宮城ゆき乃; 片岡邦重; 戸澤譲; 伏原和久; 高橋征司; 山下哲
  Volume：92nd, 2019
  J-Global ID：202002283188587712
• Quantitative assessment of drug side effect on hERG channels based on artificial bilayer lipid membranes and cell-free expression system　　　　　　　　　　　　　　　
  横田澪央; 加藤美生; 山浦大地; 常田悠介; 佐藤まどか; 但木大介; 小宮麻希; 山本英明; 井上遥; 戸澤譲; 庭野道夫; 平野愛弓
  電子情報通信学会技術研究報告, Volume：118, Number：461(CPM2018 101-120), 2019
  ISSN：0913-5685, J-Global ID：201902219959543041
• パラゴムノキの天然ゴム生合成マシナリを構成する因子の相互作用と機能相関　　　　　　　　　　　　　　　
  小島幸治; 山口真琴; 石井智樹; 廣森美樹; 和氣駿之; 山下哲; 戸澤譲; 山口春彦; 井之上ゆき乃; 伏原和久; 中山亨; 高橋征司
  日本植物生理学会年会(Web), Volume：60th, First page：435 (WEB ONLY), 2019
  Japanese
  J-Global ID：201902226936188372
• パラゴムノキの天然ゴム合成反応におけるゴム粒子の役割　　　　　　　　　　　　　　　
  小島幸治; 廣森美樹; 山家史大; 石井智樹; 和氣駿之; 山下哲; 戸澤譲; 山口晴彦; 井之上ゆき乃; 伏原和久; 中山亨; 高橋征司
  日本農芸化学会東北支部大会プログラム・講演要旨集, Volume：153rd, First page：51, 22 Sep. 2018
  Japanese
  J-Global ID：201802235741194636
• Stable formation of an artificial cell membrane sensor and its application to sensing drug side effects　　　　　　　　　　　　　　　
  平野 愛弓; 但木 大介; 山浦 大地; 荒田 航平; 大堀 健; 井上 遥香; 馬 騰; 山本 英明; 戸澤 譲; 庭野 道夫
  Volume：118, Number：9, First page：37, Last page：40, 19 Apr. 2018
  Japanese
  ISSN：0913-5685, CiNii Articles ID：40021538724, CiNii Books ID：AN10012954
• 非光合成性珪藻類葉緑体トリオースリン酸輸送体の基質特異性と紅藻類由来葉緑体の進化　　　　　　　　　　　　　　　
  神川 龍馬; Daniel Moog; 野澤 彰; 戸澤 譲
  Mar. 2018
• 微細加工チップ中に形成した脂質二分子膜への無細胞合成イオンチャネルの包埋と薬物副作用評価の定量　　　　　　　　　　　　　　　
  横田澪央; 加藤美生; 吉田美優; 山浦大地; 荒田航平; 但木大介; 山本英明; 戸澤譲; 庭野道夫; 平野愛弓; 平野愛弓
  Volume：67th, 2018
  J-Global ID：201802223449111951
• 微細加工チップ中に形成した脂質二分子膜への無細胞合成イオンチャネルの包埋と薬物副作用の定量　　　　　　　　　　　　　　　
  加藤美生; 吉田美優; 山浦大地; 荒田航平; 但木大介; 山本英明; 戸澤譲; 庭野道夫; 平野愛弓; 平野愛弓
  Volume：65th, 2018
  J-Global ID：201802240025100442
• 微細加工シリコンチップ中に再構成した脂質二分子膜における無細胞合成hERGチャネルの薬物副作用の定量　　　　　　　　　　　　　　　
  常田悠介; 加藤美生; 山浦大地; 荒田航平; 但木大介; 小宮麻希; 山本英明; 井上遥香; 戸澤譲; 庭野道夫; 平野愛弓; 平野愛弓
  Volume：79th, 2018
  J-Global ID：201802269986179413
• 脂質粒子におけるシス型プレニルトランスフェラーゼの発現と精製の試み　　　　　　　　　　　　　　　
  佐田京香; 山口晴彦; 宮城ゆき乃; 片岡邦重; 戸澤譲; 伏原和久; 高橋征司; 山下哲
  日本生化学会大会(Web), Volume：91st, First page：ROMBUNNO.1P‐109 (WEB ONLY), 2018
  Japanese
  J-Global ID：201902246837938521
• 天然ゴム生合成マシナリを構成するNgBRファミリータンパク質の機能解析　　　　　　　　　　　　　　　
  高畑佳佑; 今泉璃城; 山口晴彦; 宮城ゆき乃; 片岡邦重; 戸澤譲; 伏原和久; 高橋征司; 山下哲
  日本生化学会大会(Web), Volume：91st, First page：ROMBUNNO.1P‐108 (WEB ONLY), 2018
  Japanese
  J-Global ID：201902282333864882
• 大腸菌無細胞翻訳系を用いた天然ゴム合成酵素の再構成　　　　　　　　　　　　　　　
  小島幸治; 山下哲; 戸澤譲; 山口春彦; 井之上ゆき乃; 伏原和久; 中山亨; 高橋征司
  日本植物生理学会年会(Web), Volume：59th, First page：ROMBUNNO.P.408 (WEB ONLY), 2018
  Japanese
  J-Global ID：201802240902763361
• Evolutionary cross talk between the non-photosynthetic plastids and the cytosol of Nitzschia　　　　　　　　　　　　　　　
  Ryoma Kamikawa; Daniel Moog; Akira Nozawa; Yuzuru Tozawa
  The IVth Molecular Life of Diatoms, Jul. 2017, [Reviewed]
  English
• 支持脂質二重膜に再構成したイオンチャネルの膜内分子配向と分子分布　　　　　　　　　　　　　　　
  手老 龍吾; 福本 幸平; 鈴木 祐也; 吉田 美優; 平野 愛弓; 庭野 道夫; 野澤 彰; 戸澤 譲
  Volume：117, First page：37, Last page：40, 2017
• 電位依存性K⁺チャネル KAT1の支持脂質二重膜への再構成過程と分子配向 (ケミカルセンサ バイオ・マイクロシステム合同研究会 統合化バイオサーキットおよびエレクトロバイオロジー)　　　　　　　　　　　　　　　
  鈴木 祐也; 野澤 彰; 戸澤 譲; 手老 龍吾
  Volume：2016, Number：32, First page：5, Last page：10, 21 Dec. 2016
  Japanese
  CiNii Articles ID：40021052866, CiNii Books ID：AA1146975X
• パラゴムノキの天然ゴム合成酵素の分子解析
  山下哲; 山下哲; 山口晴彦; 和氣駿之; 山家史大; 青木裕一; 宮城ゆき乃; 伏原和久; 戸澤譲; 中山亨; 高橋征司
  イソプレノイド研究会例会講演要旨集, Volume：26th, First page：22, 20 Sep. 2016
  Japanese
  J-Global ID：201602228274455885
• 日本型イネから見出した4‐HPPD阻害型除草剤抵抗性遺伝子の有効性　　　　　　　　　　　　　　　
  山崎明彦; 関野景介; 山田祐司; 前田英郎; 村田和優; 加藤浩; 吉田均; 川岸万紀子; 廣瀬咲子; 谷口洋二郎; 川田元滋; 大島正弘; 戸澤譲
  育種学研究, Volume：18, First page：27, 21 Mar. 2016
  Japanese
  DOI：https://doi.org/10.1270/jsbbr.18.27
  DOI ID：10.1270/jsbbr.18.27, ISSN：1344-7629, J-Global ID：201602281603394823
• シロイヌナズナPAPS輸送体PAPST2の解析　　　　　　　　　　　　　　　
  名樂仁; 井上寛之; 佐々木孝行; 山本洋子; 戸澤譲; 澤崎達也; 野澤彰
  Volume：39th, 2016
  J-Global ID：201702264255851067
• 電位依存性K+チャネル KAT1の支持脂質二重膜への再構成過程と分子配向　　　　　　　　　　　　　　　
  鈴木 祐也; 野澤 彰; 戸澤 譲; 手老 龍吾
  Volume：CHS-16, First page：033, 2016
• 細菌によるトランス‐3‐ヒドロキシ‐L‐プロリン代謝経路の解明
  渡辺誠也; 谷本佳彰; 山内清司; 戸澤譲; 渡部保夫
  日本生化学会大会(Web), Volume：87th, First page：3P-180 (WEB ONLY), 2014
  Japanese
  J-Global ID：201502287720005863
• Complete in vitro system for the functional analysis of membrane transport proteins　　　　　　　　　　　　　　　
  野澤 彰; 戸澤 譲
  Volume：72, Number：3, First page：190, Last page：191, 2014
  Japanese
  ISSN：0914-8981, CiNii Articles ID：40020102360, CiNii Books ID：AN10028025
• 細菌によるコラーゲン由来ヒドロキシプロリン代謝に関する新知見
  渡辺誠也; 谷本佳彰; 西脇寿; 戸澤譲; 渡部保夫
  日本生化学会大会(Web), Volume：86th, First page：3T18P-04(1P-155) (WEB ONLY), 2013
  Japanese
  J-Global ID：201402256710473630
• 細菌由来の新規L‐ヒドロキシプロリン代謝経路の解明　　　　　　　　　　　　　　　
  渡辺誠也; 森本大地; 福森文康; 四宮博人; 西脇寿; 河田美幸; 笹井雄貴; 戸澤譲; 渡部保夫
  日本生化学会大会(Web), Volume：85th, First page：WEB ONLY 3T03-09, 2012
  Japanese
  J-Global ID：201402272214685428
• マダイI型インターフェロンの機能解析　　　　　　　　　　　　　　　
  太田史; 加藤千絵; 上田祐輔; 岩井俊治; 三浦智恵美; 三浦猛; 戸澤譲; 戸澤譲
  Volume：2011, 2011
  J-Global ID：201102208485910620
• マハタ組み換えインターフェロンの効果
  上田祐輔; 太田史; 伊藤克敏; 岩井俊治; 浦崎慎太郎; 山下浩史; 戸澤譲; 三浦智恵美; 三浦猛
  日本水産学会大会講演要旨集, Volume：2010, First page：52, 22 Sep. 2010
  Japanese
  J-Global ID：201002298368180124
• ppGpp biosynthesis systems and their physiological roles in plants　　　　　　　　　　　　　　　
  Tozawa Yuzuru; Kasai Koji
  Regulation of Plant Growth & Development, Volume：45, Number：2, First page：119, Last page：124, 2010
  For the last fifty years, unique signal molecule, ppGpp, has been received broad attention by a number of molecularbiologists. Recently, it has been revealed that the ppGpp biosynthesis system also exists in chloroplasts of photosynthetic eukaryotes including land plants. For elucidating physiological functions of the ppGpp in plants, currently some different approaches such as biochemical analysis of synthetic enzymes, genetic analysis of mutant plants, and in vitro reconstitution analysis of ppGpp-signaling in chloroplast extracts, have been utilized. This review overviews recent progress and perspectives in understanding of plant ppGpp-signaling systems.
  The Japanese Society for Chemical Regulation of Plants, Japanese
  DOI：https://doi.org/10.18978/jscrp.45.2_119
  DOI ID：10.18978/jscrp.45.2_119, ISSN：1346-5406, CiNii Articles ID：110008006480, CiNii Books ID：AA11550064
• Production of membrane proteins through the wheat-germ cell-free technology.　　　　　　　　　　　　　　　
  Volume：607, First page：213, Last page：218, 2010
  DOI：https://doi.org/10.1007/978-1-60327-331-2_17
  DOI ID：10.1007/978-1-60327-331-2_17
• Protein engineering accelerated by cell-free technology.　　　　　　　　　　　　　　　
  Volume：607, First page：85, Last page：99, 2010
  DOI：https://doi.org/10.1007/978-1-60327-331-2_9
  DOI ID：10.1007/978-1-60327-331-2_9
• ppGpp biosynthesis systems and their physiological roles in plants　　　　　　　　　　　　　　　
  Tozawa Yuzuru; Kasai Koji
  The Japanese Society for Chemical Regulation of PlantsRegulation of Plant Growth & Development, Volume：45, Number：2, First page：119, Last page：124, 2010
  For the last fifty years, unique signal molecule, ppGpp, has been received broad attention by a number of molecularbiologists. Recently, it has been revealed that the ppGpp biosynthesis system also exists in chloroplasts of photosynthetic eukaryotes including land plants. For elucidating physiological functions of the ppGpp in plants, currently some different approaches such as biochemical analysis of synthetic enzymes, genetic analysis of mutant plants, and in vitro reconstitution analysis of ppGpp-signaling in chloroplast extracts, have been utilized. This review overviews recent progress and perspectives in understanding of plant ppGpp-signaling systems.
  The Japanese Society for Chemical Regulation of Plants, Japanese
  DOI：https://doi.org/10.18978/jscrp.45.2_119
  DOI ID：10.18978/jscrp.45.2_119, ISSN：1346-5406, CiNii Articles ID：110008006480, CiNii Books ID：AA11550064
• Production of membrane proteins through the wheat-germ cell-free technology.　　　　　　　　　　　　　　　
  Humana Press Inc., Totowa, NJ.Methods in Molecular Biology, Volume：607, First page：213, Last page：218, 2010
  DOI：https://doi.org/10.1007/978-1-60327-331-2_17
  DOI ID：10.1007/978-1-60327-331-2_17
• Protein engineering accelerated by cell-free technology.　　　　　　　　　　　　　　　
  Humana Press Inc., Totowa, NJ.Methods in Molecular Biology, Volume：607, First page：85, Last page：99, 2010
  DOI：https://doi.org/10.1007/978-1-60327-331-2_9
  DOI ID：10.1007/978-1-60327-331-2_9
• ゴカイ類が有する有機物分解能の分子生物学的解析　　　　　　　　　　　　　　　
  伊藤克敏; 野崎真奈; 太田史; 戸澤譲; 三浦智恵美; 三浦猛
  Volume：2010, 2010
  J-Global ID：201002228400588590
• Functional analysis of a novel ppGpp synthetase, YwaC, and regulatory mechanism for the dimerization of ribosome, in Bacillus subtilis　　　　　　　　　　　　　　　
  Kazumi Tagami; Kenta Masuda; Marie Maehashi; Yoshitaka Hirohata; Masaki Yoshida; Haruko Kuroiwa; Tsuneyoshi Kuroiwa; Hideaki Nanamiya; Yuzuru Tozawa; Shenghao Liu; Yasushi Kageyama; Katsutoshi Ara; Katsuya Ozaki; Fujio Kawamura
  GENES & GENETIC SYSTEMS, Volume：84, Number：6, First page：455, Last page：455, Dec. 2009
  English, Summary international conference
  ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000277071300090
• [Membrane protein production using wheat germ cell-free system].　　　　　　　　　　　　　　　
  Nozawa A; Tozawa Y; Sawasaki T; Endo Y
  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Volume：54, Number：12 Suppl, First page：1443, Last page：1447, Sep. 2009, [Reviewed]
  Japanese
  ISSN：0039-9450, CiNii Articles ID：40016800815, CiNii Books ID：AN00140437, PubMed ID：21089567
• コムギ胚芽無細胞系を用いた膜タンパク質生産　　　　　　　　　　　　　　　
  Volume：54, Number：12, First page：1443, Last page：1447, 2009
• Functional Analysis of Membrane Proteins by Cell-free Translation System　　　　　　　　　　　　　　　
  TOZAWA Yuzuru; NOZAWA Akira; GENJI Takahisa
  KAGAKU TO SEIBUTSU, Volume：47, Number：2, First page：98, Last page：103, 2009
  Japan Society for Bioscience, Biotechnology, and Agrochemistry, Japanese
  DOI：https://doi.org/10.1271/kagakutoseibutsu.47.98
  DOI ID：10.1271/kagakutoseibutsu.47.98, ISSN：0453-073X, CiNii Articles ID：10026434746, CiNii Books ID：AN00037573
• Functional Analysis of Membrane Proteins by Cell-free Translation System　　　　　　　　　　　　　　　
  Volume：47, Number：2, First page：98, Last page：103, 2009
• Functional analysis of a novel ppGpp synthetase, YwaC, in Bacillus subtilis　　　　　　　　　　　　　　　
  Kazumi Tagami; Tetsuya Wada; Masaki Yoshida; Haruko Kuroiwa; Tsuneyoshi Kuroiwa; Hideaki Nanamiya; Yuzuru Tozawa; Fujio Kawamura
  GENES & GENETIC SYSTEMS, Volume：83, Number：6, First page：513, Last page：513, Dec. 2008
  English, Summary international conference
  ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000265228400140
• Diversity of ppGpp biosynthesis pathways and physiological functions in plants and bacteria　　　　　　　　　　　　　　　
  TOZAWA Yuzuru; KASAI Koji; NANAMIYA Hideaki
  Bioscience & industry, Volume：66, Number：6, First page：293, Last page：297, 01 Jun. 2008
  Japanese
  ISSN：0914-8981, CiNii Articles ID：10024745422, CiNii Books ID：AN10028025
• Metabolic Engineering of The Phenylpropanoid Pathway in Arabidopsis thaliana Using Foreign Gene　　　　　　　　　　　　　　　
  Yun Choong-Soo; Matsuda Fumio; Yamamoto Tomio; Nozawa Akira; Saito Kazuki; Tozawa Yuzuru
  Plant and Cell Physiology Supplement, Volume：2008, Number：0, First page：142, Last page：142, 2008
  The first step of phenylpropanoid pathway in plants starts from phenylalanine. In plant cells, phenylalanine is converted to courmaric acid via cinnamic acid, then courmaric acid branches to various secondary metabolites. One more important branch point of this pathway is flavanone that is converted to flavone or isoflavone. In this study, we attempted to alter this pathway by using tyrosine ammonia-lyase (TAL), which directly converts tyrosine to coumaric acid instead of phenylalanine, and parsley flavone synthase (FNS). Expressions of the introduced genes were examined by RT-PCR or RNA gel-blot analysis, and the resulted transgenic plants were analyzed by LC-MS and HPLC to investigate profiles of their secondary metabolites. We observed accumulations of specific flavonoids that were dependent on the expression of transgene, TAL or FNS. We thus confirmed the in vivo functions of each trasgene that can alter the biosynthetic pathway of phenylpropanoids in <I>Arabidopsis</I>.
  日本植物生理学会
  CiNii Articles ID：130006992950
• Regulation of transcription of an rrn operon by relA, yjbM and ywaC in Bacillus subtilis　　　　　　　　　　　　　　　
  Murakami Kana; Natori Yousuke; Nanamiya Hideaki; Tozawa Yuzuru; Kawamura Fujio
  GENES & GENETIC SYSTEMS, Volume：82, Number：6, First page：548, Last page：548, Dec. 2007
  English, Summary international conference
  ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000254238200204
• Improvement of analysis system for plant membrane proteins by using cell-free protein synthesis system.　　　　　　　　　　　　　　　
  Akira Nozawa; Takuji Miyata; Yuzuru Tozawa
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S88, Last page：S88, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922700347
• Functional analysis of arogenate dehydratase of rice by means of wheat-embryo cell-free protein expression system　　　　　　　　　　　　　　　
  Koji Kasai; Yuki Obayashi; Tetsuya Yamada; Takuya Kanno; Kyo Wakasa; Yuzuru Tozawa
  PLANT AND CELL PHYSIOLOGY, Volume：48, First page：S219, Last page：S219, 2007
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000245922701353
• Functional roles of plastid SIG1 for gene expression of photosystem I components　　　　　　　　　　　　　　　
  Tozawa Yuzuru; Teraishi Masayoshi; Sasaki Tadamasa; Sonoike Kintake
  Plant and Cell Physiology Supplement, Volume：2007, Number：0, First page：73, Last page：73, 2007, [Lead, Corresponding]
  DOI ID：10.14841/jspp.2007.0.073.0, ISSN：0032-0781, CiNii Articles ID：130006990401, Web of Science ID：WOS:000245922700135
• Function analysis of relA gene and it's paralogous genes, yjbM and ywaC, in Bacillus subtilis.　　　　　　　　　　　　　　　
  Kana Murakami; Yousuke Natori; Makiko Sato; Hideaki Nanamiya; Fujio Kawamura; Yuzuru Tozawa
  GENES & GENETIC SYSTEMS, Volume：81, Number：6, First page：442, Last page：442, Dec. 2006
  English, Summary international conference
  ISSN：1341-7568, eISSN：1880-5779, Web of Science ID：WOS:000245138300140
• Chloroplast ppGpp synthesis system　　　　　　　　　　　　　　　
  PROTEIN, NUCLEIC ACID AND ENZYME, Volume：50, Number：14, First page：1860, 2005
• The Wheat Germ Cell-Free Expression System　　　　　　　　　　　　　　　
  Humana Press Inc.Methods in Molecular Biology, Volume：310, First page：131, Last page：144, 2005
• 葉緑体のppGpp合成系　　　　　　　　　　　　　　　
  Volume：50, Number：14, First page：1860, 2005
• Functional analysis of rice virescent-2 gene essential for chloroplast development　　　　　　　　　　　　　　　
  H Sugimoto; K Kusumi; A Yoshimura; S Kikuchi; Y Tozawa; K Iba
  PLANT AND CELL PHYSIOLOGY, Volume：45, First page：S208, Last page：S208, 2004
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000220592700820
• "Magic spot" synthase in chloroplasts　　　　　　　　　　　　　　　
  Y Tozawa
  SEIKAGAKU, Volume：75, Number：7, First page：600, Last page：604, Jul. 2003
  Japanese
  ISSN：0037-1017, Web of Science ID：WOS:000184465400005
• Cell-free Protein Synthesis by Wheat Germ Extracts　　　　　　　　　　　　　　　
  戸澤 譲; 澤崎 達也; 遠藤 弥重太
  Volume：45, Number：1, First page：3, Last page：8, 2003
  DOI：https://doi.org/10.5940/jcrsj.45.3
  DOI ID：10.5940/jcrsj.45.3, ISSN：0369-4585
• For the regulation and utilization of primary and secondary metabolisms in the tryptophan biosynthetic pathways　　　　　　　　　　　　　　　
  Wakasa Kyo; Tozawa Yuzuru
  Regulation of Plant Growth & Development, Volume：38, Number：2, First page：249, Last page：254, 2003
  The Japanese Society for Chemical Regulation of Plants, Japanese
  DOI：https://doi.org/10.18978/jscrp.38.2_249
  DOI ID：10.18978/jscrp.38.2_249, ISSN：1346-5406, CiNii Articles ID：110001845961, CiNii Books ID：AA11550064
• Cell-free Protein Synthesis by Wheat Germ Extracts　　　　　　　　　　　　　　　
  Yuzuru TOZAWA; Tatsuya SAWASAKI; Yaeta ENDO
  Nihon Kessho Gakkaishi, Volume：45, Number：1, First page：3, Last page：8, 2003
  With the sequencing of the genomes of various species, attention has turned to the structure, properties, and functional activities of proteins. However, rapid progress in the area of proteomics is premised on the availability of sufficient amounts of a large number of proteins. Here we described a novel cell-free system from wheat embryos for the high-throughput screening/ synthesis of gene products. Our system should open up many possibilities in the post-genome era.
  The Crystallographic Society of Japan, Japanese
  DOI：https://doi.org/10.5940/jcrsj.45.3
  DOI ID：10.5940/jcrsj.45.3, ISSN：0369-4585, CiNii Articles ID：10012821848, CiNii Books ID：AN00188364
• “Magic spot” synthase in chloroplasts　　　　　　　　　　　　　　　
  Volume：75, First page：600, Last page：604, 2003
• Expression analysis of chloroplast proteins in virescent mutants (v(1), v(3)) of rice　　　　　　　　　　　　　　　
  K Kusumi; T Kouno; H Sugimoto; Y Tozawa; A Yoshimura; S Kikuchi; K Iba
  PLANT AND CELL PHYSIOLOGY, Volume：44, First page：S57, Last page：S57, 2003
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000181914300223
• Chracterization of transgenic rice containing high level of tryptophan　　　　　　　　　　　　　　　
  WAKASA K.; YAMADA T.; TOZAWA Y.; KOMATSU A.; NEMOTO H.; HASEGAWA H.; SAKURAI M.; TERAKAWA T.
  Volume：4, First page：217, Last page：217, 26 Aug. 2002
  Japanese
  ISSN：1344-7629, CiNii Articles ID：10010403302, CiNii Books ID：AA11317194
• Analysis of rice transformants with the mutated anthranilate synthase gene driven by five different promoters for the development of selection marker　　　　　　　　　　　　　　　
  A Komatsu; A Yoshikawa; H Hasegawa; M Kawagishi-Kobayashi; Y Nishizawa; K Sugimoto; Y Tozawa; K Wakasa
  PLANT AND CELL PHYSIOLOGY, Volume：43, First page：S121, Last page：S121, 2002
  English, Summary international conference
  ISSN：0032-0781, Web of Science ID：WOS:000174726400437
• Hokko Chem. Industry,C.Res.Lab.　　　　　　　　　　　　　　　
  HASEGAWA H.; TOZAWA Y.; TERAKAWA T.; WAKASA K.
  Volume：2, Number：2, First page：111, Last page：111, 25 Sep. 2000
  Japanese
  ISSN：1344-7629, CiNii Articles ID：10006112058, CiNii Books ID：AA11317194
• Functional analysis of the altered ribosome from the antibiotic high-producing Bacillus Subtilis.　　　　　　　　　　　　　　　
  Inaoka Takashi; Tozawa Yuzuru; Ochi Kozo
  Volume：12, First page：253, Last page：253, 2000
  Japanese
  CiNii Articles ID：110002922464, CiNii Books ID：AN10549378
• Effect of relA and ribosome mutations on antibiotic production in Bacillus subtilis.　　　　　　　　　　　　　　　
  Kasai Koji; Inaoka Takashi; Tozawa Yuzuru; Ochi Kozo
  Volume：12, First page：253, Last page：253, 2000
  Japanese
  CiNii Articles ID：110002922465, CiNii Books ID：AN10549378
• The analysis of the function of the gene for the chloroplast RNApolymerase σ factor from Oryza sative　　　　　　　　　　　　　　　
  TSUNOYAMA Yuichi; TOZAWA Yuzuru; WAKASA Kyo
  Volume：39, First page：S95, Last page：S95, May 1998
  English
  ISSN：0032-0781, CiNii Articles ID：10003751857, CiNii Books ID：AA0077511X
• Roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation　　　　　　　　　　　　　　　
  Volume：14, First page：88, Last page：91, 1996
• Roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation　　　　　　　　　　　　　　　
  EXPERIMENTAL MEDICINE, Volume：14, First page：88, Last page：91, 1996
• INVOLVEMENT OF PROTEIN-KINASE C-EPSILON IN GLUCOCORTICOID-INDUCED APOPTOSIS IN MURINE THYMOCYTES　　　　　　　　　　　　　　　
  M IWATA; R ISEKI; Y TOZAWA; K SATO; Y ZHAO; Y OHOKA
  FASEB JOURNAL, Volume：8, Number：5, First page：A786, Last page：A786, Mar. 1994
  English, Summary international conference
  ISSN：0892-6638, Web of Science ID：WOS:A1994ND19701169
• INVOLVEMENT OF CALCIUM-INDEPENDENT LIPID-STIMULATED KINASE IN GLUCOCORTICOID-INDUCED APOPTOSIS IN THYMOCYTES　　　　　　　　　　　　　　　
  M IWATA; R ISEKI; Y TOZAWA; K SATO
  JOURNAL OF IMMUNOLOGY, Volume：150, Number：8, First page：A31, Last page：A31, Apr. 1993
  English, Summary international conference
  ISSN：0022-1767, Web of Science ID：WOS:A1993KX95600170

• Chemical Genomics: Reviews and protocols. Methods in Molecular Biology　　　　　　　　　　　　　　　
  2005
• Chemical Genomics: Reviews and protocols. Methods in Molecular Biology　　　　　　　　　　　　　　　
  Humana Press, 2005
• 日本結晶学会誌　　　　　　　　　　　　　　　
  2003

• Japanese Society for Plant Cell and Molecular Biology

• 無細胞タンパク質合成系の保健衛生および畜産分野への応用　　　　　　　　　　　　　　　
  2005 - 2005

• Molecular dissection of plant specialized metabolism machineries　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), 12 Apr. 2023 - 31 Mar. 2028
  Tohoku University
  Grant amount(Total)：203710000, Direct funding：156700000, Indirect funding：47010000
  Grant number：23H05470
• 未知ストリゴラクトン代謝経路におけるHIS1/HSLファミリーの機能的役割の解明　　　　　　　　　　　　　　　
  01 Apr. 2022 - 31 Mar. 2025
  Grant amount(Total)：17420000, Direct funding：13400000, Indirect funding：4020000
  Grant number：22H02270
• Understanding the core structures and dynamics of membrane protein complexes related in productions of valuable compounds from plants　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2021 - 31 Mar. 2024
  Kanazawa University
  Grant amount(Total)：17810000, Direct funding：13700000, Indirect funding：4110000
  Grant number：21H02115
• 固液界面機能と脂質二重膜を用いた人工生体膜反応場の構築　　　　　　　　　　　　　　　
  01 Apr. 2020 - 31 Mar. 2024
  Grant amount(Total)：17550000, Direct funding：13500000, Indirect funding：4050000
  Grant number：20H02690
• 単一イオンチャネル分子／バイオ二次元物質ハイブリッド膜の機能解析と応用　　　　　　　　　　　　　　　
  01 Apr. 2019 - 31 Mar. 2023
  Grant amount(Total)：45500000, Direct funding：35000000, Indirect funding：10500000
  Grant number：19H00846
• Study on molecular regulatory mechanisms in thermo-sensitive male sterility of rice based on cell-free translation system　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2019 - 31 Mar. 2022
  Tozawa Yuzuru, Saitama University
  Grant amount(Total)：4290000, Direct funding：3300000, Indirect funding：990000
  The rapid global warming has a great impact on the production of major cereals such as rice, and the high-temperature conditions during the flowering season not only deteriorate the quality of seeds but also sometimes cause sterility. This problem of high-temperature sterility is one of the important issues to be solved in order to maintain food production in the future. The principal investigators predicted transcription factors that it might be involved in high-temperature male sterility in rice. To conduct a functional analysis of these transcription factors at the protein level, the candidates were synthesized and purified as a full-length protein by using a cell-free translation system based on the wheat germ extract. It has been confirmed that one transcription factor, OsMyb80, shows specific binding to the regulatory region of the gene CYP703, which is essential for pollen formation.
  Grant number：19K05822
• Development of screening system for high-affinity artificial ligand against memblane proteins　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), 30 Jun. 2017 - 31 Mar. 2019
  Nemoto Naoto; NAKAI JYUNICHI, Saitama University
  Grant amount(Total)：6500000, Direct funding：5000000, Indirect funding：1500000
  Over the past 20-year, bio-pharmaceuticals as molecular target drugs, especially antibody drugs have been given some effective ways to treat difficult disorder diseases (i.e., cancer). On the other hand, any antibodies against G protein- coupled receptors (GPCR) which are around 50% of drug targets, have not been developed because high titer of antibodies can not be obtained. Recently, peptide aptamers have been attracting much attention because of its thermal stability and low-cost to overcome antibody’s problems. In this study, we have developed a novel method to select some peptide aptamers against GPCR using cDNA display method that is one of powerful evolutionary molecular engineering tools.
  Grant number：17K19471
• Elucidation of molecular mechanism in ATP-depending translation activity in plant seed cells　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, 01 Apr. 2016 - 31 Mar. 2019
  Tozawa Yuzuru; Suzuki Kakeru, Saitama University
  Grant amount(Total)：3640000, Direct funding：2800000, Indirect funding：840000
  In this study, we have established a new plant cell-free translation system based on rice callus extracts. We revealed that the rice cell-free system allows efficient protein expression in the absence of GTP. We confirmed generation of low level GTP from GMP, which is degradation product of mRNA by endogenous nuclease. On the other hand, By using polyuridylic acid (polyU), which contains only uridine but not guanine nucleotide, as a template, we demonstrated that translation elongation activity of the system is not affected by the lack of GTP in the reaction. This GTP-independence in the translation elongation activity is different from yeast cell-free system, and seems to be unique to plant seed cell system.
  Grant number：16K14665
• Analysis of effects of lipids on function and structure of membrane proteins　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2015 - 31 Mar. 2018
  Nozawa Akira; TOZAWA Yuzuru, Ehime University
  Grant amount(Total)：5070000, Direct funding：3900000, Indirect funding：1170000
  In this study, by using mitochondrial carriers and a lipid, cardiolipine, which is known to interact with the mitochondrial carrier, as a model membrane protein-lipid system, we clarified roles of lipids against the function and structure of the membrane protein. In this study, the effect of cardiolipin on transport activity of yeast and malaria parasite mitochondrial carriers was analyzed. As a result, it was clarified that addition of cardiolipin increases the transport activity of the mitochondrial carriers localized in mitochondrial membrane, but such a effect was not observed in the mitochondrial carriers present in other membrane systems.
  Grant number：15K07006
• Cooperative effects of natural-derived lipids and membrane proteins in lipid bilayer membranes　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), 01 Apr. 2015 - 31 Mar. 2018
  Tero Ryugo; FUKUMOTO Kohei; SUZUKI Yuya; NIIYAMA Yuya; OKAMOTO Yoshiaki, Toyohashi University of Technology
  Grant amount(Total)：17680000, Direct funding：13600000, Indirect funding：4080000
  This project aimed to elucidate the active state created by a plurality of membrane proteins and surrounding lipid molecules, and relating phenomena using a cell membrane model system. We reconstructed ion channel proteins that were obtained from cultured cells and cell-free protein synthesis system, in supported lipid bilayers (SLBs). Distribution and molecular structures of lipids and membrane proteins were observed by fluorescence microscopy and atomic force microscopy. We propose a model to explain the mechanism of reconstitution by clarifying the role of cell membrane derived components in SLB. We revealed that the role of intra-membrane microdomains in the reconstitution process. It is revealed that the molecular orientation of the channel protein expressed by cell-free synthesis varies depending on the reconstitution process.
  Grant number：15H03768
• Establishment of post-translational modification system in the cell free protein system　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), 01 Apr. 2013 - 31 Mar. 2017
  Izumi Atsushi; YUZURU TOZAWA
  Grant amount(Total)：4030000, Direct funding：3100000, Indirect funding：930000
  We introduced a simple method to synthesize and purify a membrane-associated protein in the wheat germ cell free system as a model production system. We used ferrochelatase from Saccharomyces cerevisiae, catalyzing the insertion of ferrous ion into the tetrapyrrole center of protoporphyrin IX, as a model protein. The luminol-horse radish peroxidase assay indicated that the active ferrochelatase was only synthesized in the presence of assolectin liposomes. The Accudenz density gradient centrifugation experiment indicated that ferrochelatase clearly localized at the liposome fraction and the complex of the synthesized protein with liposomes can be simply separated from soluble protein fraction by a single step centrifugation.
  Grant number：25850071
• 細胞内物質代謝系とオルガネラ膜輸送体の共進化　　　　　　　　　　　　　　　
  01 Apr. 2014 - 31 Mar. 2016
  Grant amount(Total)：7670000, Direct funding：5900000, Indirect funding：1770000
  Grant number：26117717
• Functional analysis of transporters regulating translocation of energy metabolites in rice organelle　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2012 - 31 Mar. 2015
  NOZAWA Akira; TOZAWA Yuzuru, Ehime University
  Grant amount(Total)：4810000, Direct funding：3700000, Indirect funding：1110000
  We improved a method for functional analysis of transporter proteins based on liposome-supplemented cell-free system. In the new system, detection range of transport activity against non-specific background activity was improved by up-regulated synthetic efficiency of membrane proteins in cell-free reaction. By using this method, we analyzed several transporter proteins localized in plant organelle. Substrate specificity of sugar-phosphate transporters in rice and iceplant plastids and sulfate metabolite transporter in Arabidopsis plastids.
  Grant number：24580094
• Characterization of plant stringent regulation by introducing artificial ppGpp-signal system　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 01 Apr. 2012 - 31 Mar. 2015
  TOZAWA Yuzuru; OGAWA Atsushi
  Grant amount(Total)：5460000, Direct funding：4200000, Indirect funding：1260000
  In this study, we have performed experiments for clarifying function of ppGpp in plant chloroplasts. By establishing in vitro translation system from isolated chloroplasts, we have demonstrated that chloroplast translation activity is inhibited by ppGpp. Further, we have shown that activity of guanylate kinase, a key enzyme for GTP biosynthesis in chloroplasts, is potently inhibited by ppGpp. We have thus proven that ppGpp regulates chloroplast translation and GTP biosynthesis in plants. Moreover, we have established cyanobacterial genetic system that enables induction of protein-expression by theophylline administration based on riboswithch system. The system has also been applied to transformation of tobacco chloroplast genome.
  Grant number：24570054
• 細胞内物質代謝系の統合と変遷に伴うミトコンドリア輸送体の獲得と共進化　　　　　　　　　　　　　　　
  01 Apr. 2012 - 31 Mar. 2014
  Grant amount(Total)：5720000, Direct funding：4400000, Indirect funding：1320000
  Grant number：24117516
• Study on stringent response in plant chloroplasts　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 2009 - 2011
  TOZAWA Yuzuru; YUKAWA Yasushi; NOMURA Yuhta, Ehime University
  Grant amount(Total)：4680000, Direct funding：3600000, Indirect funding：1080000
  Chloroplasts are derived from symbiosis of photosynthetic bacteria, and have ppGpp-mediated signal transduction system. In order to clarify the functional roles of ppGpp-signal in chloroplasts, we have investigated functions of ppGpp in the protein synthesis. Here we have established in vitro translation system based on extracts of isolated pea chloroplasts, and demonstrated that ppGpp directly regulates protein synthesis activity of chloroplasts in vitro.
  Grant number：21570047
• Calcium signal transduction network in plant innate immunity　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), 2008 - 2010
  NAKAHIRA Yoichi; YUKAWA Yasushi; SHIINA Takashi; YOSHIOKA Hirofumi; SUGIURA Masahiro; TOZAWA Yuzuru; KUCHITU Kazuyuki, Kyoto Prefectural University
  Grant amount(Total)：31980000, Direct funding：24600000, Indirect funding：7380000
  Mitochondria and chloroplasts are not only energy source for eukaryotic cells, but also play additional important roles to modulate cellular functions. For example, mammal mitochondria contribute to the initiation of apoptosis, diseases and aging. Plant chloroplast also serves several important functions in plant immunity, including biosynthesis of salicylic acid (SA) and the generation of reactive oxygen species (ROS), key signaling molecules in plant immune response. Interestingly, previous studies have shown that chloroplasts contain proteins involved in plant immune responses. Furthermore, several pathogen effectors target chloroplasts to suppress chloroplast-derived defense signals. Therefore, it is anticipated that chloroplasts play a role in plant innate immunity. However, how chloroplasts sense pathogen signals and control immune signaling network remains unclear. This study illustrates a previously unknown crosstalk between chloroplasts and innate immunity through chloroplast Ca^<2+> signaling in plants.
  Mitochondrial Ca^<2+> homeostasis plays a key role in apoptosis by switching Ca^<2+>-induced release of cytochrome C (caspase cofactor). However, very little is known about Ca^<2+> homeostasis in chloroplasts. Here, we report that pathogen associated molecular pattern (PAMP) signals induce a rapid increase in the stromal Ca^<2+> concentration. Pharmacological studies demonstrate that the cytoplasmic Ca^<2+> signal is transferred to the chloroplasts and induces the stromal Ca^<2+> transient. We further demonstrate that chloroplast-localized putative Ca^<2+> binding protein, CAS is involved in the generation of stromal Ca^<2+> signals. These studies suggest a novel mechanism relaying PAMP signals into chloroplasts at the beginning of the immune process.
  The PAMP-induced stromal Ca^<2+> signaling may control or activate the innate immune response. Thus, we examined the role of CAS in plant innate immunity. Remarkably, CAS deficient plants (cas-1) are compromised in broad range of innate immune responses including PAMP-induced basal defense and R-gene mediated hypersensitive cell death. It is unlikely that the compromised immunity of cas-1 plants is caused by limited cellular resources, since CAS deficient mutant lines exhibit normal phenotype and photosynthetically competent. Furthermore, a comprehensive analysis of plant hormone dynamics demonstrated that CAS is specifically required for PAMP-induced accumulation of SA, a key signal molecule in plant immune responses. These results suggest that chloroplast protein CAS acts as a positive regulator of plant innate immunity through SA-dependent signaling.
  CAS regulates SA biosynthesis most likely at transcriptional level. It is therefore anticipated that chloroplasts release retrograde signals that control defense gene expression during immune responses. PAMP induces expression of numerous defense related genes in nucleus. Using a genome wide transcriptome analysis we show that CAS is indispensable for the global induction of defense gene expression before SA biosynthesis starts, suggesting that CAS is involved in the chloroplast-to-nucleus retrograde signaling and mediates chloroplast control of plant innate immunity. Chloroplast-derived ^1O_2^- signaling has been implicated with the control of stress defense-related gene expression. Here we show that most genes downregulated in CAS deficient plants were enriched for ^1O_2^- responsive genes, providing evidence for a role of CAS in ^1O_2^- signaling to control nuclear-encoded defense genes.
  Collectively this study illustrates previously unknown mechanisms that coordinate chloroplast functions with nuclear-encoded defense gene expression. Upon infection, PAMP signals are relayed to chloroplasts through the cytoplasmic Ca^<2+>-induced stromal Ca^<2+> signaling pathway. This study further implicates a role of ^1O_2^- retrograde signaling as underlying mechanisms of chloroplast control of plant innate immunity. Chloroplast protein CAS plays a critical role in both processes. Identification of the chloroplast-dependent immune pathway may provide a novel strategy for the disease control of plants.
  Grant number：20200060
• 無細胞翻訳系を応用した植物膜輸送体の機能解析　　　　　　　　　　　　　　　
  2008 - 2009
  Grant amount(Total)：4400000, Direct funding：4400000
  Grant number：20053016
• Molecular mechanisms of the response of photosynthesis via redox regulation of translation　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 2007 - 2008
  NISHIYAMA Yoshitaka; TOZAWA Yuzuru; HISABORI Toru
  Grant amount(Total)：4550000, Direct funding：3500000, Indirect funding：1050000
  Grant number：19570043
• Study on functional roles of calcium-dependent stringent factor in plant chloroplasts　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 2007 - 2008
  TOZAWA Yuzuru; NANAMIYA Hideaki, Ehime University
  Grant amount(Total)：4420000, Direct funding：3400000, Indirect funding：1020000
  Grant number：19570042
• 無細胞蛋白質合成技術を応用した植物膜輸送タンパク質の機能解析　　　　　　　　　　　　　　　
  2006 - 2007
  Grant amount(Total)：5200000, Direct funding：5200000
  Grant number：18056016
• Molecular mechanisms of the response of photosynthesis to oxidative stress　　　　　　　　　　　　　　　
  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), 2005 - 2006
  NISHIYAMA Yoshitaka; TOZAWA Yuzuru, Ehime University
  Grant amount(Total)：3500000, Direct funding：3500000
  Ph00otoinhibition of photosystem II (PSII) is due to the imbalance between the rate of photodamage to PSII and the rate of the repair of damaged PSII. Photodamage is initiated by the direct effects of light on the oxygen-evolving complex and, thus, photodamage to PSII is unavoidable. Studies of the effects of oxidative stress on photodamage and subsequent repair have revealed that reactive oxygen species (ROS) act primarily by inhibiting the repair of photodamaged PSII and not by damaging PSII directly. Thus, strong light has dual effects on PSII ; it damages PSII directly and it inhibits the repair of PSII via production of ROS. Investigations of the ROS-induced inhibition of repair have demonstrated that ROS suppress the synthesis de novo of proteins that are required for the repair of PSII, such as the D1 protein. Moreover, analysis of polysomes has determined that a primary target for inhibition by ROS is the elongation step of translation. Investigations using a cyanobacterial translation system in vitro have revealed that elongation factor G might be the primary target, within the translational machinery, of inhibition by ROS. Here we present a new paradigm for the molecular action of ROS in photoinhibition.
  Grant number：17570040